Flow cytometry within reach.

Transcription

1

2 Affordable, but more than capable. The Accuri C6 Flow Cytometer is flow cytometry at its best. Its affordable price makes it accessible beyond compare. The C6 has a foot print small enough to fit most any lab and is designed to allow you to do your research when you like. Affordably priced, minimal set-up. Accuri designed the C6 Flow Cytometer from the ground up to be a full-featured, affordable instrument that is accessible to everyone. Major areas of innovation include a unique lowpressure pumping system to drive fluidics rather than a more expensive pressurized system. The optical bench is compact and employs components that have evolved to be smaller, more robust, and less expensive. This smaller optical bench needs no alignment so the C6 is ready to use in less than 2 hours after receipt. High quality components plus innovations enable Accuri to offer the C6 at a price that is affordable for most labs. A flow cytometer that fits your lab. Flow cytometer affordability is a combination of factors including purchase price, routine maintenance, service contract price, time required to learn the system or teach others, and the need to hire a dedicated operator. These costs are reduced or eliminated with the C6. The system is so easy to operate and maintain that a dedicated operator is not required. New C6 users can be trained in less than 30 minutes. The C6 employs laboratory-grade water for sheath fluid so commercial sheath fluids are not needed. Maintenance is made simple with automatic rinse and decontamination upon instrument shutdown. No room renovations are required to install a C6. It fits easily on the benchtop and uses standard electrical service. Research on your schedule. The C6 is small enough to fit into every lab. It can even be placed in a laminar flow hood. It is available, at your fingertips, anytime you need to check cell concentration, viability or phenotype. Think of the times when results from one experiment required rapid follow-up, yet you had no access to a flow cytometer for weeks. With a C6 Flow Cytometer in your lab, you can follow up quickly on your experimental results and move forward with your research. applications: Immunophenotyping Bacteria Analysis Cell Cycle Analysis Proliferation Assays Apoptosis DNA Analysis Target Multiplexing The small footprint, low maintenance costs and ease of use make the C6 an excellent choice for any lab... Core Lab Manager, Ohio State University Gene Expression Viability Absolute Cell Counts Intracellular Cytokines Membrane Potential Yeast and Algae Analysis Phagocytosis

3 Powerful, but still at your fingertips. The Accuri C6 Flow Cytometer features 2 lasers, 2 scatter and 4 fluorescence detectors. Designed to work well with current protocols and reagents, the C6 is a high performance flow cytometer, simplified. High performance in a small package. The C6 Flow Cytometer is small and portable yet has the power to do your research. Four fluorescence and two scatter detectors give you the power you have come to expect in a flow cytometer. An industry-standard, sheath-focused core facilitates high performance while enabling event rates of up to 10,000 events/second and sample concentrations over 5x10 6 cells/ml. The C6 even measures the volume pulled from your sample so that cell concentration can be measured without the need for adding beads to samples or performing time-consuming hemocytometer counts. 2 Lasers, 2 scatter and 4 fluorescence channels. The C6 is equipped with a blue and a red laser, two scatter detectors, and four fluorescence detectors with interference filters optimized for the detection of FITC, PE, PerCP-Cy5.5 and APC. Since the specifications and performance of the C6 are similar to that of the market-leading flow cytometers, you can use your current reagents and protocols. The C6 has a fully digital data collection system, allowing complete flexibility to display and analyze data post-collection, without any alteration of your original data file. Works with current protocols and reagents. You have spent years developing protocols and reagents you can depend on for your research. So we developed the C6 to use the same reagents you currently use on the market leading flow cytometers. Since the C6 fluidics system is nonpressurized, any brand of sample tube that is 12x75 mm or smaller can be used. This includes microfuge tubes and tubes made of polypropylene or polystyrene. FCS 3.1 compatible file format. The C6 Flow Cytometer allows you to export CFlow files in FCS 3.1 format for seamless importation of your data into most 3rd party flow cytometry software programs including FCS Express and FlowJo. I was truly amazed with the C6 it very dramatically showed the advantage of having such a wide dynamic range. Core Facility Scientist, University of Florida

4 Simple, but in a very scientific way. CFlow software has an intuitive user interface designed specifically with the researcher in mind. And the Accuri C6 Flow Cytometer has pre-optimized detectors with over 6 decades of dynamic range. Ease of use makes for better research. CFlow software is easy to learn and teach. Accuri spent hundreds of hours observing researchers using flow cytometers to develop the intuitive design of CFlow software. The user interface is so easy to learn that most current flow cytometer users are collecting and analyzing data in less than 30 minutes. CFlow uses a tabbed approach to access functions such as Collect, Analyze and Statistics. As a result, most user software options and instrument controls are clearly visible on screen, not hidden under layers of menus. Even the novice flow cytometer user can quickly learn to use the C6 Flow Cytometer from a simple quick start guide. a very easy-to-use flow cytometer. It s ideal for inexperienced users because you don t have the risk of not collecting the data in the right way, you can always re-analyse it. Flow Cytometry Core Facility, University College Dublin Compatible with commonly used software. Creating a presentation of your flow data could not be simpler. You can drag and drop plots into MS Office applications, copy and paste statistics into spreadsheets, making data reporting easy. You can also quickly export CFlow data to FCS 3.1 data files for analysis in your favorite 3rd party flow cytometry software. Pre-optimized detector settings, no loss of valuable data. Often after spending weeks developing valuable scientific material, an inexperienced flow cytometrist loses data due to incorrect settings. The Accuri C6 Flow Cytometer has pre-optimized detector settings and an increased dynamic range of over 6 decades, making all of your data available all of the time. In addition, Accuri added the CFlow Zoom function, which allows you to visualize your data at any scale, enabling the precision settings of gates and regions. All your data, at any time. The combination of digital data collection and more than six decades of dynamic range provides unrivaled flexibility in your data analysis. Gating strategies and fluorescence compensation values can be set during, or after, data collection. New users can collect data using a pre-set template, then analyze the data later with more expert help. Data collected on a C6 Flow Cytometer can be re-analyzed at any time if gating or compensation errors are discovered, or in light of new research findings.

5 Automated, but still simple and affordable. The Accuri CSampler takes flow cytometry to the next level, by adding simple, reliable and easy-to-use automation to the C6 Flow Cytometer System. And best of all, CSampler is less than half the price of other flow cytometry automation systems. Meet growing research needs. Now, you can prepare your samples in 96-well plates and take them straight to the flow cytometer, without the need to transfer them into tubes first, saving time, additional steps and operator error. Accuri s optional CSampler accessory is compatible with 48- and 96-well plates and deep-well plates, and is also supplied with a 24-tube rack for standard 12x75 mm tubes. Plus, the CSampler adds minimal footprint to the C6, about three feet square for the pair, keeping your bench top free for other uses. Analyze a 96-well plate in less than ninety minutes. CSampler analyzes a 96-well plate in less than ninety minutes with walk-away convenience. Whether you are standing right by the CSampler or across the lab, easy-to-read system status messages keep you up to date. Intuitive, easy-to-use software. CFlow Sampler software offers flexibility for plate set-up and experimental design. Multiple experiments can be run in a single plate or tube rack, with settings applied to specific samples or an entire plate and the ability to construct templates for re-use. The priority interrupt function allows you to pause a run, replace a plate with the 24-tube rack and process samples that have to be dealt with immediately. Then, the original plate can be put back on the CSampler and the run will resume where it left off. Priced to fit your budget needs. CSampler is easy to afford at less than half the price of other systems. Add it to the purchase of a C6 Flow Cytometer with CFlow Plus software or, if funding is tight, build your ideal system and spread out the purchases over several budget cycles. The cost is the same. You can also add a CSampler to an existing C6. Bottom line, it s not just the price of the instrument that contributes to cost. CSampler also helps reduce the use of precious samples, reagents and time.

6 Our mission is to enable the power of flow cytometry to proliferate worldwide to every lab working with cells or beads. We started by listening to scientists like you and then designing a flow cytometer from the ground up incorporating your needs. We worked hard to provide the performance required for most experiments, while making the C6 easier to use, simple to maintain, and affordable for everyone. Accuri s innovation in fluidics, optics, electronics and software, coupled with stateof-the-art components, result in a cytometer that is powerful, robust, compact, affordable and now automated to provide even more convenience and a further reduction of operating costs. We also developed an intuitive user interface that can be mastered in minutes. And we ve done all of this so you can focus on one thing: advancing your science, in your lab. Accuri Cytometers, Inc. 173 Parkland Plaza Ann Arbor, MI USA Tel: Fax: CustomerService@AccuriCytometers.com Accuri Cytometers (Europe) Ltd. 56 Edison Road St. Ives, Cambs PE27 3LF, UK 2009 Accuri Cytometers Accuri, C6 Flow Cytometer and CFlow are registered trademarks of Accuri Cytometers, Inc. CSampler and Flow cytometry within reach are trademarks of Accuri Cytometers, Inc. Tel: +44 (0) Fax: +44 (0) EuroTech@AccuriCytometers.com