Microbiology and Molecular Biology

Transcription

1 Molecular Biology Workflow Solutions Complete solutions for your Molecular Biology Workflows Most molecular biology practices start with nucleic acid purification including nucleic acid quantification and quality analysis followed by end point or real-time PCR. Whether your goal is to clone a gene of interest, study RNA interference or perform gene expression analysis, we offer an extensive range of instruments, reagents and consumables to accelerate your molecular biology research. Unparalleled nucleic acid purification Our revolutionary patented KingFisher TM magnetic separation technology lets you process virtually any sample from any source. With four systems to choose from, KingFisher TM system provides the performance, flexibility and speed for your budget, application and throughput requirements. With optimised KingFisher Kits, you can easily extract blood DNA, total RNA, cells and tissue DNA, plant DNA and viral nucleic acids. Optionally, our product portfolio includes a wide range of GeneJET TM Spin Columns for cost effective DNA or RNA isolation from various starting materials for lower throughput needs. Flexibility and performance for nucleic acid analysis The Multiskan TM GO Microplate Spectrophotometer is a handy tool for virtually any photometric research application. Reading microplates, cuvettes and the µdrop TM Plate for microlitre-scale measurements, Multiskan GO supports any sample volume and throughput requirement for measuring absorbance. The NanoDrop TM instruments were the first microvolume UV-Vis spectrophotometers designed specifically for the life scientist. The innovative sample retention system* (footnote: *patents US and US ) enables direct measurements of 1-2 µl of sample without the need for dilutions or cuvettes. A simple pipette, measure and wipe clean process has fundamentally changed the way scientists measure the concentration and purity of their DNA, RNA and protein samples. High performance PCR and qpcr solutions The Piko TM Thermal Cyclers deliver top performance with twice the speed and only half the size of regular thermal cyclers. The Arktik TM Thermal Cycler is a reliable and flexible PCR instrument for everyday requirements in any laboratory. It is suitable for a wide range of applications from individual reactions to highthroughput projects. The range of polymerases and master mixes deliver top performance over a range of applications, from standard PCR to high-fidelity and direct PCR. The PikoReal TM Real-Time PCR System is designed to fit into your lab, even when bench-top space is limited. Offering an exceptionally small footprint, this qpcr instrument is ideal for personal use and light enough for field applications. The qpcr reagent portfolio includes qpcr master mixes for both probe and SYBR Greenbased chemistries. You can choose optimized mixes for both fast and standard protocols, and reduce the risk of pipetting errors and carry-over contamination. The pre-designed qpcr gene-expression assays offer a convenient solution for reliable gene-specific quantification with a single assay. 345

2 Molecular Biology Workflow Solutions PREPARE SAMPLES Centrifuges and sample storages DNA/RNA PURIFICATION Manual and automated nucleic acid purification > > DNA/RNA ANALYSIS UV/Vis spectrophotometers Centrifuges Exceptional capacity, performance and ease-of use in a remarkably small footprint Auto-LockTM rotor exchange provides secure push-button application versatility Lightweight and durable performance from Fiberlite carbon fiber rotors One-handed, certified sample protection with ClickSealTM Biocontainment Lids Note : See Centrifuge section on pages GeneJET Nucleic Acid Purification Kits Spin columns with trusted silica membrane technology Isolate DNA and RNA with superior purity High yields from various sources DNA and RNA ready-to-use in downstream applications Cost effective price/prep Note : See the following pages for more details Multiskan GO Microplate Spectrophotometer Ideal for DNA/RNA, ELISA, enzyme assays and more Reads microplates, cuvettes and the µ Drop Plate for microlitre-scale measurements Stand-alone or PC control in multiple languages Automation compatible Note : See the following pages for more details Cold Storage Storage of reagents and kits (qpcr, extraction buffers, primers, etc) that require stable temperatures and conditions to be maintained for maximum efficacy Multiple storage capacities and configurations to meet customer needs Models come standard with easy-to-read displays, multiple alarms, access ports and remote monitoring for sample security and peace of mind Multiple options and accessories available, including enzyme bins, extra shelves and 4 20 ma outputs Glass doors are standard on many models allowing for easy reagent/kit/sample identification and retrieval Manual defrost freezer available in enzyme storage configuration Note : See Cold Storage section on pages KingFisher Product Family Automated and flexible systems with optimised DNA/RNA kits Wide range of sample types including blood, serum, cells, tissues and plant material can be used Excellent performance and reproducibility Walk-away solutions for any throughput needs Note : See the following pages for more details µ Drop Plate for microlitre-scale measurements Detection of nucleic acids from microgrammes to milligrammes per millilitre Measurement of up to 16 samples simultaneously Measurement of volumes as low as 2 µl 346 Online:

3 Molecular Biology Workflow Solutions > PCR / qpcr PCR and qpcr instruments, plastics and reagents LIQUID HANDLING Manual and automated liquid handling > > AUTOMATION Laboratory automation Piko and Arktik Thermal Cyclers Piko Time, reagent, space and energy savings with the highest performance. PCR in as little as 15 minutes. Green PCR with reduced energy consumption and plastic waste Arktik Reliability and flexibility with interchangeable blocks (48/48, 96 and 384) and gradient function Note : See the following pages for more details PikoReal Real-Time PCR System Compact, high performance qpcr instrument enabling savings in the running costs Easy to use software with unrestricted access and Virtual Pipetting Tool Green qpcr with reduced energy consumption and plastic waste Note : See the following pages for more details PCR and qpcr Reagents High-fidelity and routine DNA polymerases, kits and master mixes Phusion, Phire, DreamTaq Probe and SYBR Green qpcr master mixes for fast and standard qpcr DyNAmo and Luminaris Pre-designed Solaris Assays for human and mouse gene expression studies Reverse transcriptases and kits for first strand cdna synthesis, RT-PCR and RT-qPCR Maxima, RevertAid, and Verso Note : Please contact us for further details PCR and qpcr Plastics Individual tubes and strip tubes 24, 48, 96 and 384-well plates and Piko UTW PCR Plates Sealing options from cap strips and adhesive seals to storage plate sealing Storage plates Barcoding for reliable sample tracking Note : Please contact us for further details Multidrop TM Reagent Dispensers Versatile plate formats, plate heights and a wide volume range Fast and precise dispensing even at small volumes Easy and convenient to use via a large color Note : See the following pages for more details Versette TM Automated Liquid Handler User interchangeable 96- and 384-channel pipetting heads with a total volume Ìl Optimized performance with selection of D.A.R.TsT M tips which provides unique sealing properties User-friendly programming options with icon-based onboard touch screen or Versette ControlMate M PC Software Space saving compact design with dual-level deck configuration Note : See the following pages for more details Manual and Electronic Pipettes and Pipette Tips F1-ClipTip TM Pipetting System Secure tip attachment The only tips that will not loosen or fall off mid-application An airtight seal on every channel For consistent, reproducible results Lowest tip attachment & ejection forces Reduced risk of repetitive stress injury (RSI) ClipTip Low Resolution technology - Minimise liquid retention to enable maximum sample recovery Note : See the following pages for more details Matrix TM Electronic Pipettes Single and multi-channel electronic pipettes Step-based programming makes it logical, intuitive and easy to create programs Adjustable tip spacing: Flexibility to transfer multiple samples between many types of labware Trigger-based operation and ejection to reduce strain on thumb Five variable speeds for each step of the protocol (aspirate, dispense or mix) RapidStak TM Microplate Stacker Most reliable microplate stacker in its class Two instrument loading for ultimate versatility Automate up to 150 plates with the 2x model Works with a wide variety of Thermo Scientific readers, washers, dispensers and plate sealers Note : See the following pages for more details Orbitor TM Microplate Mover Reliable benchtop automation Flexible plate handling: deep well, lids, stacked or random access storage Safe: collision and plate sensing stops the mover Easy to use: computer operated, wizards guide you through teaching Note : See the following pages for more details 347

4 DNA / RNA Purification Kits and Reagents GeneJET The GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types. The kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis. Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis. RNA yields vary between different species, tissues, and age of tissue sample. Product Highlights : Fast: procedure completed in 20 min following cell lysis Convenient: protocol does not include clogging-prone lysate filtration step High yield: up to 65 µg of high purity and high integrity RNA from 5 mg plant sample Compatible with wide variety of samples (leaves, roots, sprouts and other parts of various plants) Applications Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including: RT-PCR RT-qPCR Northern blotting Nuclease protection assay Note : For Research Use Only. Not for use in diagnostic procedures. Specifications Sample Type (General) : High Throughput Compatibility : Downstream Application : Product Size : Plant Samples Not High Throughput-Compatible (Manual) Nuclease Protection Assays, Real-Time Quantitative PCR (qpcr), Nucleic Acid Labeling, Reverse Transcriptase PCR (RT-PCR), cdna Library Construction, Northern Blotting 50 preps Ordering Cat No. K0801 K0802 Description 50 Preps 250 Preps Note : Other GeneJET Kits are available, please ask for details 348 Online:

5 Kingfisher Nucleic Acid Purification System Meet The KingFisher family Our revolutionary patented magnetic separation technology lets you process virtually any sample from any source for the ultimate in isolation of nucleic acids, proteins and cells. With four systems to choose from, these KingFisher TM systems provide the performance, flexibility and speed for your budget, application and throughput requirements : KingFisher Flex : With high-throughput or processing volume up to 5 ml, Flex system offers truly versatile purification of nucleic acids and proteins. The range of volumes is µl depending on the magnet head with 96- or 24-well format. Use predefined protocols or customize your own for special applications. KingFisher Duo : The newest to the KingFisher family, Duo system delivers advanced functionality in a compact, mid-throughput capacity instrument for isolation applications. Its small footprint and big functionality, including traceability and data management, make it a perfect fit for research and routine laboratories. Two protocols can run sequentially without interruption, raising throughput up to 24 samples per load. Duo system also includes large volume processing up to 5 ml. KingFisher ml : The economical choice for easy operation up to 15 samples. Processing volumes from 50 to 1000 µl carried out in tube strips. KingFisher : The first in the family, KingFisher system allows to purify smallscale samples economically. Run up to 24 samples in µl. All purification and processing steps, programmed using simple push-button operation, are carried out in microstrips. KingFisher Pure DNA / RNA Kits : Now, with optimised KingFisher Pure purification kits, you can easily perform extraction of DNA or RNA from blood, cells and tissues or plant samples, as well as viral NA and plasmid DNA purification. KingFisher instruments, software, kits and consumables deliver unparalleled performance. Pierce Magnetics Beads TM : The high-performance, iron oxide, superparamagnetic particles are available for immunoprecipitation, affinity purification as well as creating custom magnetic particles. Instrument KingFisher Flex KingFisher Duo KingFisher ml KingFisher Samples/run (24) Working volume (µl)

6 Kingfisher - Ordering Information Cat No. Description KingFisher instruments KingFisher, V, 50/60 Hz KingFisher ml, V, 50/60 Hz KingFisher Duo, V, 50/60 Hz KingFisher Flex with 96 PCR head, V, 50/60 Hz KingFisher Flex with 96 KF head, V, 50/60 Hz KingFisher Flex with 96 deep-well head, V, 50/60 Hz KingFisher Flex with 24 deep-well head, V, 50/60 Hz Consumables for KingFisher KingFisher tip comb, 50 pcs KingFisher plate 100 ÌL, 50 pcs KingFisher plate 200 ÌL, 50 pcs Consumables for KingFisher ml KingFisher ml tip comb, 800 pcs KingFisher ml tube, 900 pcs KingFisher ml Combi 240, (tubes and tip combs), for 240 samples Consumables for KingFisher Duo KingFisher Duo 12-tip comb, for Microtiter 96 deep-well plate, 50 pcs Microtiter deep-well 96 Plate, 50 pcs KingFisher Duo 6-tip combs, for KingFisher Flex 24 deep-well plate, 48 pcs KingFisher Flex 24 deep-well Plate, 50 pcs KingFisher Duo elution strip, 40 pcs KingFisher Duo Combi pack for Microtiter 96 deep-well plate (tips combs, plates and elution strips for 96 samples) Consumables for KingFisher Flex KingFisher 96 tip comb for PCR magnets, 80 pcs KingFisher 96 tip comb for KF 96 magnets, 100 pcs KingFisher 96 tip comb for deep-well magnets, 10 x 10 pcs/box KingFisher 96 KF plate, 48 pcs Microtiter deep-well 96 Plate, 50 pcs Microtiter deep-well 96 Plate, sterile, 50 pcs KingFisher Flex 24 deep-well tip comb and plate, 50 pcs of each KingFisher Flex 24 deep-well Plate, 50 pcs KingFisher Flex 24 deep-well Plate Sterile, 50 pcs Cap Mat for DW 96 Plate, 50 pcs Cap Mat for DW 96 Plate, sterile, 25 pcs Cat No. Description KingFisher Pure DNA/RNA Kits KingFisher Pure DNA Blood Kit, 96 preps KingFisher Pure DNA Blood Kit, 384 preps KingFisher Pure RNA Blood Kit, 96 preps KingFisher Cell and Tissue DNA Kit, 96 preps KingFisher Cell and Tissue DNA Kit, 24 x 96 preps KingFisher Pure RNA Tissue Kit, 96 preps KingFisher Pure RNA Tissue Kit, 384 preps KingFisher Pure DNA Plant Kit, 96 preps KingFisher Pure DNA Plant Kit, 384 preps KingFisher Pure RNA Plant Kit, 96 preps KingFisher Pure RNA Plant Kit, 384 preps KingFisher Pure Viral NA Kit, 96 preps KingFisher Pure Viral NA Kit, 384 preps KingFisher Pure Plasmid Kit, 96 preps KingFisher Pure Plasmid Kit, 384 preps Pierce Magnetic Beads for proteomics applications Pierce Protein A/G Magnetic Beads, 1 ml Pierce Protein A/G Magnetic Beads, 5 ml Pierce NHS-Activated Magnetic Beads, 1 ml Pierce NHS-Activated Magnetic Beads, 5 ml Pierce Classic Magnetic IP/Co-IP Kit, 40 reactions Pierce Crosslink Magnetic IP/Co-IP Kit, 40 reactions Pierce Direct Magnetic IP/Co-IP Kit Pierce Streptavidin Magnetic Beads, 1 ml Pierce Streptavidin Magnetic Beads, 5 ml Pierce Glutathione Magnetic Beads, 4 ml Pierce Glutathione Magnetic Beads, 20 ml Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit, 96 reactions Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit, Trial Size, 24 reactions HisPur Ni-NTA Magnetic Beads, 2 ml HisPur Ni-NTA Magnetic Beads, 10 ml Magnetic ChIP Kit, 30 reactions ChIP-grade Protein A/G Magnetic Beads, 5mL 350 Online:

7 Multiskan GO UV/Vis Microplate and Cuvette Spectrophotometer freedom for any photometric research application The Multiskan GO microplate spectrophotometer is a handy tool for virtually all photometric research applications. Select any measurement wavelength from low UV to visible with just a few keyboard clicks to measure both cuvettes and microplates, all according to your assay and throughput requirements. You can control Multiskan GO as stand-alone with straightforward internal software for quick and simple measurements or easily set up even the most demanding applications with the truly intuitive SkanIt Software for PC control. Both options offer a selection of multiple languages for operation. Multiskan GO offers Freely selectable wavelengths from nm for the demands of various assays Both microplate and cuvette reading for any throughput requirements Very fast plate measurements and a full spectrum of a sample in less than 10 seconds High quality data guaranteed by extensive self diagnostics A unique power save function for reduced energy consumption Visual internal software on a large colour screen for quick measurements Easy and logical assay setup for demanding assays with the powerful SkanIt Software A selection of eight different operation languages Multiskan GO with cuvette Flexibility and performance for various photometric applications Multiskan GO microplate spectrophotometer supports endpoint, kinetic and spectral scanning assays. It reads 96- and 384-well microplates with and without lids, and all major cuvette types. The reading speed of Multiskan GO is exceptionally fast; all wells in a 96-well plate are measured in less than 6 seconds, and a full 384 well plate is measured in just 10 seconds. A broad wavelength range with the UV area, pathlength correction as well as fast spectral scanning makes it an ideal tool for any photometric research application, including DNA, RNA and protein analysis. Both the microplate chamber and cuvette holder are equipped with temperature control up to 45 C to enable temperature sensitive applications such as enzyme kinetics or cellular assays. Shaking speed can be selected to ensure that the samples in the microplate stay homogeneous. Easy to swap assays with free wavelength selection Wavelength selection in Multiskan GO is done by using an advanced monochromator system. Any wavelength between 200 and 1000 nm can be selected at any time. Using the spectral scanning feature, the whole spectrum of a sample can be scanned in 1 nm increments to allow identification of the optimal measurement wavelength for a new assay. The complete spectrum of a sample can be scanned in just 10 seconds. Additionally, the narrow measurement bandwidth ensures excellent spectral resolution. Stand-alone use for quick measurements The Multiskan GO can be controlled without a computer using the visual internal software and its large colour display making it convenient for quick and simple measurements of both microplates and cuvettes. Ready-made sessions for measuring DNA and RNA concentrations and easy-to-use formulae for ratiometric or background correction measurements are available for cuvettes in the internal software. Any measurement data can easily be saved on a USB memory stick for transfer to a computer for further processing. Visual and comprehensive assay setup with SkanIt Software The logical and truly user-friendly interface of the SkanIt Software makes the instrument control and assay setup easy, even for advanced applications. The SkanIt Software offers a comprehensive selection of inbuilt calculations, including quantitative curve fit, qualitative classification, kinetic calculations, spectral reduction and parallel line analysis (PLA) to make data reduction very straightforward. In addition, any measurement or calculated data in the SkanIt Software can be automatically exported to Excel with just one mouse click. The same language selection as for the internal software is available also for the SkanIt Software. It simply speaks your language. 351

8 Multiskan GO UV/Vis Microplate and Cuvette Spectrophotometer freedom for any photometric research application High quality data and consistent performance The optical system in Multiskan GO has been engineered to ensure first rate performance and high quality results. The design incorporates a dual beam optical system which includes an internal reference channel ensuring consistent results during any measurement condition. Extensive automatic self diagnostics at instrument start-up verifies all major instrument functionalities, such as measurement positioning, lamp and monocromator, as well as measurement electronics operation. In addition, continuous runtime control of optical and mechanical functions guarantee stable day-to-day and yearon-year performance and reliability. Multiskan GO is designed so you can trust the integrity of your results, always. Meets RoHS Directive Built using the highest quality components, Multiskan GO conforms to the RoHS (Restriction of Hazardous Substances) directives. Specially designed for automation Increase assay throughput by integrating the Multiskan GO with stackers and automated systems. The Multiskan GO s plate carrier is specially-designed for convenient robotic arm access to allow microplate gripping in portrait and landscape configurations. The SkanIt Software automation interface enables easy integration with automated systems. * In SkanIt Software, the calculation is performed in compliance with the European Pharmacopoeia guidelines. The built-in monochromator makes it easy to select any measurement wavelength Energy-saving for a better environment Multiskan GO features a unique power save functionality that is automatically activated when the instrument is on but not in use. By pressing any instrument key or by using the SkanIt Software, the power is instantly resumed and Multiskan GO is ready for use. When the power save is activated, it reduces the energy consumption of the instrument over 70%. Easy to use internal software for quick plate or cuvette measurements Visual and logical SkanIt Software with eight language versions USB port for easy data transfer Multiskan GO accepts versatile cuvettes Typical applications DNA or RNA quantitation and purity Protein assays Enzyme assays Kinetic assays Immunoassays Cell proliferation and cytotoxicity assays Apoptosis assays Reporter gene assays GPCR assays 352 Online:

9 Multiskan GO - Technical Specifications and Ordering Information Technical Specifications Feature Wavelength selection Light source Wavelength range Read-out range Bandwidth Monochromator Xenon flash lamp nm with 1 nm steps Up to 4 Abs < 2.5 nm Plate Cuvette 450 nm Abs, 2% (96-well plate) Abs, 2% (beam window >/= 2 mm) 450 nm 1.0% Abs (0-2.0 Abs) 1.0% Abs (0-2.0 Abs) 2.0% ( Abs) 2.0% ( Abs) 450 nm SD < Abs or CV < 0.5% (Precision mode) SD < Abs or CV < 0.5% SD < Abs or CV < 1.0% (Fast mode) Plates / Cuvettes 96- and 384-well plates 12.5 (W) x 12.5 (D) x (H) mm Measurement speed 6 s with 96-well plate - (from A1 back to A1) 10 s with 384-well plate - Shaking Linear Spectral scanning speed 10 s from 200 to 1000 nm with 1 nm steps Incubation range From ambient + 2 C to 45 C User interfaces Stand-alone use: 4.5-inch color display and keypad PC control: SkanIt Software USB connections PC Memory device port for data export External printer (HP PCL5 compatible) Mains input V (50/60 Hz) Max power consumption < 110 W Power save consumption < 2.5 W Dimensions (H x W x D) 260 x 285 x 430 mm 10.2 x 11.2 x 16.9 in. Weight 10.8 kg [23.8 lbs.] PC requirements for SkanIt Software Minimum system requirements Dual-core processor, 2 GB RAM, 5 GB free hard disk space, USB, CD-ROM drive, mouse or equivalent, XGA monitor Operating system Microsoft Windows Ordering Information Cat. No. Description Multiskan GO without Cuvette V, 50/60 Hz Multiskan GO with Cuvette V, 50/60 Hz 353

10 Piko Thermal Cycler Complete a PCR protocol in less than 15 minutes with the Piko Thermal Cycler, which meets the highest criteria in thermal performance at twice the speed and half the size of other thermal cyclers. This is achieved using novel technical advances that allow significant reduction in PCR run times and overall size of the instrument. The Piko Thermal Cycler is an ideal solution for both convential and fast PCR applications. Piko Thermal Cyclers offer you Best-in-class thermal performance Consistent results from well-towell due to extremely short settling times and high temperature uniformity across the block. Time savings PCR in as little as 15 minutes enabled by fast ramp rates and quick settling times. Reagent savings Repeatable yields from as little as 5 µl when using UTW vessels and heat sealers. Space savings The smallest footprint available - fits into a tiny bench space. Energy saving Short protocols and low wattage requires only a quarter of the power consumption of typical thermal cyclers. Repeatable sealing to prevent sample evaporation Automatic heated lid provides consistent and tight sealing from run-to-run. Two formats: 24- and 96-well blocks Piko Thermal Cyclers are available in two different block configurations: 24-well and 96-well. 24-well cyclers accept all standard low profile single tubes, and 8-tube strips with flat caps as well as 24-well Piko PCR Plates. The 96-well instrument utilises 96-well Piko PCR Plates, which are only a quarter of the size of a conventional microplate. Despite the small size, all Piko PCR Plates maintain industry standard well spacing and sample capacity. The 24-well and 96-well Piko PCR Plates are compatible with standard Thermo Scientific multichannel pipettes, Multidrop reagent dispensers and the Versette automated liquid handling system. Green PCR Reduced consumption Enhanced performance The unique design of the heating block and small plate format allow significant savings both in energy and plastics consumption. This helps reduce the environmental impact of your PCR. High yields and uniform results in less than 15 minutes The best-in-class thermal performance of Piko Thermal Cyclers means significant reduction of incubation times in most PCR protocols. This is due to the fast ramp rates, near-perfect temperature uniformity and incredibly short block settling time. It only takes 1 second for all the samples to reach temperature equilibrium. Accessories separately available Thermo Scientific Piko PCR Plates The Piko PCR Plates guarantee reliable results with routine and ultraquick PCR protocols. The vessel walls are half the thickness of conventional thinwalled tubes. This improves thermal transfer between the heating block and sample allowing shorter incubation times without compromising the consistency of results. Plate frames are available to assemble Piko PCR Plates into standard SBS format plate size. The Piko Plate Illuminator - Simplify sample loading The Piko Plate llluminator is a state-of-the-art plate rack that provides a simple way to track the loading of reactants by illuminating the target well(s) from below with white LED lights. The Piko Microcentrifuge The Piko Microcentrifuge is a compact centrifuge for spinning down samples. It is compatible with all 0.2 ml tubes, 8-tube strips and Piko PCR Plates. The Piko Microcentrifuge has two interchangeable rotors. Piko Studio software Piko Studio is a full-featured PC software that allows you to control the Piko Thermal Cycler remotely, backup, edit and transfer protocols. Piko Studio software Piko Studio is a full-featured PC software that allows you to control the Piko Thermal Cycler remotely, backup, edit and transfer protocols. 354 Online:

11 Piko Thermal Cycler - Technical Specifications and Ordering Information Technical Specifications Size Width 16 cm, depth 17 cm, height 23 cm Weight 4 kg (with external power supply) Power requirements V input power Block configurations 24-well (well volume ml, sample volume max 50 µl) and 96-well (well volume 0.05 ml, sample volume max 20 µl Max ramp rate > 5 C heating and > 4.5 C cooling Typical thermal uniformity +/- 0.3 C within 5 s of clock start at 95 C Thermal accuracy +/- 0.2 C Settling times Achieve thermal specifications within 1 s of start of clock Thermal range 0 C C User interface Semi-graphical and list mode programming Number of programmes Over 1000 Heated lid Fully-automated and motorized function Ordering information Cat. No, Description Quantity Piko Thermal Cyclers TCP0024 Piko Thermal Cycler, 24-well 1 TCP0096 Piko Thermal Cycler, 96-well 1 Piko Studio PST user PST0005 up to 5 users PST0010 up to 10 users Piko Plate Illuminator PIP2496 Piko Plate Illuminator 1 Piko Microsentrifuges MCP0011 Piko Microcentrifuge with 2 rotors (UK, 230V) 1 MCP0012 Piko Microcentrifuge with 2 rotors (EU, 230V) 1 Piko PCR Plates and Frames SPL well Piko PCR Plate, clear 200 plates SPL well Piko PCR Plate, clear 200 plates SFR0241 Plate Frame for 24-well Piko PCR, white. Holds 4 plates. 50 frames SFR0961 Plate Frame for 96-well Piko PCR, white. Holds 4 plates. 50 frames UTW PCR tubes with flat caps TUC0080 UTW 8-tube strip with attached, flat optical caps, clear 120 strips TUC0010 UTW individual tube with attached, flat optical cap, clear 960 tubes Sealers HSF0021 Heat sealing film, opaque, Piko size 400 sheets ASF0020 Optical adhesive film, cryo-storage compatible, Piko size 400 sheets TCS1080 Flat 8-cap strips, optical 120 strips Note: PCR vessels and frames are available in several colours. 355

12 Arktik Thermal Cycler The Arktik Thermal Cycler is a reliable and flexible PCR instrument for the everyday requirements of any laboratory. It is suitable for a wide range of applications from individual reactions to high-throughput projects. Features Interchangeable blocks with excellent thermal precision Dual 48-well block for multi-user option Reliable and easy to use, with low noise level Over-tightening protection system in the heated lid Accepts virtually all standard PCR plastics Flexibility with interchangeable blocks Interchangeable blocks allow the Arktik Thermal Cycler to be quickly and easily transformed from a 96-well to a 384-well PCR instrument. With the dual block the instrument operates as two 48-well units, enabling two independent protocols to be performed simultaneously. The blocks feature a innovative heat sink design that improves the speed and thermal uniformity of the system. Reliable and quiet instrument for demanding use State-of-the-art Peltier technology employed in heating and cooling of the blocks ensures that the instrument maintains its high level of performance in demanding use. An overtightening protection system in the heated lid simplifies setting the correct pressure for PCR vessels used. The low noise level of the instrument is an additional benefit when the instrument is placed in the laboratory working space. Ease-of-use Ease-of-use is the key feature of the Arktik Thermal Cycler s user interface. The protocol is displayed graphically, and the programming is simple with intuitive menus. Arktik Thermal Cycler - Technical Specifications and Ordering Information Feature Block configurations 96 x 0.2 ml, 384 x 0.03 ml, 2 x 48 x 0.2 ml (dual block). Interchangeable. Max ramp rate 3.0 C/s Thermal uniformity +/- 0.4 C at 90 C Thermal accuracy +/- 0.3 C at 90 C Thermal range C Heated lid Manually adjustable. Over-tightening protection system. User interface Semi-graphical and list programming Number of programmes 99 Size (W x D x H) 29 cm x 38 cm x 29 cm Weight 10.5 kg Power requirements V input power, Hz, 600 W Communication ports USB, Ethernet Ordering information Product code TCA0002 TCA0096 TCA0384 TCA4848 Description Arktik Thermal Cycler base unit, without gradient 96-well single block 384-well single block Dual block (2 x 48 wells) 356 Online:

13 PikoReal Real-Time PCR System - 24-well block format and 96-well block format The PikoReal Real PCR System is designed to fit into your lab, even when bench-top space is limited. Offering an exceptionally small footprint, this qpcr instrument is ideal for personal use and light enough for field applications. The PikoReal System delivers innovative features and outstanding performance. It is part of our complete qpcr workflow that includes sample preparation, nucleic acid purification and analysis as well as qpcr assays and reagents. Performance Innovations Proprietary technologies include an innovative block design that gives high temperature uniformity across all wells, and delivers the reliable performance that you require. A Novel Format Following Industry Standards The PikoReal System is offered as a 24-well or 96-well instrument that fits PCR plates one quarter of the size of a standard plate, while maintaining industry standards for well volumes and well spacing. qpcr For Less Less cost, less waste. Reduced plate sizes, in conjunction with proprietary Ultra Thin Wall (UTW ) PCR well technology, offer superior uniformity and significant savings in reagent, plastics and energy consumption. Designed For Flexible Use The distinctive PikoReal design minimises instrument footprint, yet offers five optical detection channels. The PikoReal instrument offers flexibility in programming and data retrieval from either a computer or USB flash drive. The intuitive software, with the Virtual Pipetting Tool, guides you with ease through plate setup. Piko format full compatibility in one quarter the size of standard plates The PikoReal Real-time PCR System is available as either a 24-well or 96- well instrument that utilises proprietary UTW Piko PCR Plates. UTW Piko PCR plate walls are half the thickness (< 0.15 mm) of conventional PCR consumables and, thus, improve the thermal transfer between block and sample. The Piko PCR plates are compatible with standard multi-channel pipettes (both 8- and 16-channel) and liquid handling robots. Four 24-well/96-well Piko PCR plates are equivalent to a standard SBS 96-well/384-well plate, respectively. The plates are compatible with standard multichannel pipettes (16-channel pipette displayed). Significant savings in energy consumption. Estimated annual energy consumption (kwh/y), including running power and idle power, of the PikoReal Real-Time PCR System compared to a conventional real-time PCR system. The calculation is based on three qpcr runs per day Green qpcr reduced consumption, less cost and lower environmental impact The unique design of the heating block and the small plate format offer significant savings in energy, reagent and plastics consumption. Using plates that are one quarter the size of standard plates produces less plastic waste and reduces the cost of your research. The power usage of the PikoReal System (maximum 200 watts) is only half that of a typical qpcr instrument, which helps to reduce the environmental impact. Low power usage is also a benefit in field applications. Significant savings in plastics consumption. Estimated annual plastics consumption (kg/y) using 96- well Piko PCR plates compared to conventional 96-well microplates. The calculation is based on three qpcr runs per day and 750 qpcr runs per year. 357

14 PikoReal Real-Time PCR System - 24-well block format and 96-well block format Fast and uniform heating and heat removal gives superior performance The unique patented block design of the PikoReal instrument ensures quick heating and heat removal over the entire plate area. Temperature homogeneity performance is outstanding at all three stages of thermal cycling- and, with UTW plastics, the target temperatures are achieved rapidly. This combination ensures uniformity of data, independent of application and protocol. Tm difference from average ( C) Well position Cq difference from average Well position The performance of the block is outstanding at all three stages of amplification (95, 60 and 72 C). The graph shows temperature measurements in 96 wells of the block. Tm value uniformity and Cq value uniformity. Ninety six replicate reactions amplifying Lambda DNA (125 pg/5 ÌL) with Thermo Scientific DyNAmo Colour Flash SYBR Green Master Mix were used to demonstrate the melting temperature (Tm) and cycle of quantification (Cq) uniformity across a heat sealed 96-well plate. Tm values (A) or Cq values(b) were subtracted from the average value of 96 replicates and plotted by well position. Technological advances enable a dynamic range of 10 logs The highly optimised and precise thermal control, combined with a sensitive optical detection system, enables determination of a wide range of target concentrations. This delivers accurate Cq values for both high- and low-copy number targets in the same run. The proprietary inventions in block and heatsink design enable fast and uniform temperature control for the entire plate area. Broad dynamic range. Four technical replicates amplifying FANK1 from plasmid DNA (148 ng 1.48 x 10-5 pg /10 µl) using Thermo Scientific Solaris qpcr Gene Expression Assay and Master Mix. Amplification and standard curve shown for 11 x 10-fold dilutions of template on PikoReal 96 Real-Time PCR System. The lowest dilution displayed is equivalent to three copies of plasmid DNA in 10 µl reaction. 358 Online:

15 PikoReal Real-Time PCR System - 24-well block format and 96-well block format A five-channel optical system brings multiplexing and flexibility to your experiments The optical system of the PikoReal instrument contains five channels, precalibrated for many commonly used dyes. New dyes can be calibrated by using the Colour Calibration Utility of the software. Multiplexing can be performed with up to four dyes; the fifth channel is dedicated to SYBR Green and HRM experiments. The five LEDs used as the instrument's light source have a long lifetime and emit constant and stable light over a broad spectrum ( nm) and require no user maintenance. Separate light sources for different excitation wavelengths reduce crosstalk and focus high light energy levels. Data is collected from all wells simultaneously with a low noise and high sensitivity CCD camera ( nm). The scanning time for all four channels is less than 10 seconds, supporting the development of fast cycling protocols for multiplexing. Schematic view of the linear shuttle of the PikoReal instrument. The channel selection of the optical system is built on a motorised linear shuttle that moves from left to right. Each of the five channel positions contains separate LED light source, reflector, excitation filter, dichroic mirror and emission filter. Quadruplicate multiplexed reactions amplifying Staphylococcus aureus, Enterococcus sp., Corynebacterium bovis DNA (50,000 to 50 copies/10 ÌL reaction; channels 1, 2, 4) and internal control (500 copies of Lambda DNA/10 ÌL reaction; channel 3) using PathoProof Mastitis PCR Assay, Complete-12 Kit on PikoReal 96 Real-Time PCR System. Channel 1 FAM TM Fluorescence Dyes 2 HEX TM, Yakima Yellow TM 3 Texas Red, ROX 4 Cy TM 5 5 SYBR TM Green List of precalibrated dyes for the PikoReal instrument. The addition of new dyes is possible using the Colour Calibration Utility of the software. The optical system and light paths of the PikoReal instrument. The light, emitted from LED (yellow arrow), passes through an excitation filter and is reflected by a set of mirrors to the samples. The light signal emitted from the samples (red arrow) travels back passing through a dichroic mirror before being filtered and detected by a CCD camera. 359

16 PikoReal Real-Time PCR System - 24-well block format and 96-well block format Easy to use The PikoReal Software offers an intuitive experience, from programming protocols and defining plate layouts to assessing your experimental results. Familiar Windows operating system-based functionality, pre-loaded protocols, visual step list, Virtual Pipetting Tool Mode, import and export functions, detachable views, and many other features get the instrument to work for you. The PikoReal Software includes an unlimited licence for multiple users. The available software modules include Absolute quantification, Melt curve analysis, Allelic discrimination, Relative quantification and High Resolution Melting (HRM). Allelic discrimination. Triplicate reactions amplifying human gdna (1 ng/20 ÌL reaction) using ABI TaqManTM SNP Genotyping Assay for rs (Assay ID: C_ _10, Gene name: Hypothetical LOC339290) and DyNAmo SNP Genotyping Master Mix on PikoReal 24 Real-Time PCR System. The results are shown both on RFU-RFU scatter plot and on plate grid. DyNAmoTM SNP Genotyping Master Mix shows the three distinct genotypes: X, XY and Y. All experiment data and setting options are shown conveniently on a single page. User modifications to analysis parameters can be seen instantly. Relative quantification. sirna targeting SEC23A and Non-Targeting sirna control (sintc) were transfected into HeLa cells at 100, 10 and 1 nm final concentrations. cdna was synthesised using Maxima First Strand cdna Synthesis Kit and amplified using Solaris qpcr Gene Expression Assays and Master Mix for detection of SEC23A and PPIB on PikoReal 96 RealTime PCR System. Knockdown was calculated using the Cq method (normalized to PPIB reference gene and sintc-treated cells) for biological and technical triplicates (graphed in ExcelTM Software). The Virtual Pipetting Tool guides you through setting up plate layouts with ease. High resolution melting (HRM) analysis. Class 4 SNP detection of three genotypes using PikoReal 96 Real-Time PCR System with Luminaris Color HRM Master Mix. Shown in the left graph are the intensity normalized melting curves and in the graph on the right side is the difference graph of the same samples. The automatic clustering algorithm has detected the three clusters: homozygous AA genotype (red curves), homozygous TT genotype (blue curves) and heterozygous AT genotype (green curves). 360 USB flash drive. The PikoReal instrument can also be controlled without a computer, using a USB flash drive for protocol loading and storing run data. Online:

17 PikoReal Real-Time PCR System - 24-well block format and 96-well block format Recommended Reagents qpcr kits for SYBR Green and probe chemistries fast qpcr DyNAmo Flash and ColourFlash qpcr Kits deliver extremely short cycling times (combined annealing and extension step of only 15 s) and are the superior choice to achieve fast qpcr. DyNAmo ColourFlash qpcr kits incorporate an innovative multicolour system that ensures correct pipetting. qpcr kits for SYBR Green and probe chemistries standard qpcr Luminaris and Luminaris Colour qpcr Master Mixes are ready-to-use solutions optimised to provide the most consistent and reproducible qpcr results. The master mixes include Hot Start Taq DNA polymerase in an optimised buffer for specific and sensitive amplification, premixed UDG for cross-contamination control. Luminaris Colour qpcr Master Mix contains colours as a pipetting aid. High-Resolution Melting analysis Luminaris Colour HRM Master Mix is an optimised solution with Hot Start Taq DNA polymerase and EvaGreen fluorescent dye for robust and reliable discrimination of the differences in melting behavior between sequence variants. SNP genotyping DyNAmo SNP Genotyping Master Mix delivers fast, high-quality SNP genotyping when using endpoint fluorescence detection. The mix utilizes a specially engineered DNA polymerase that provides reliable and reproducible discrimination of SNP alleles. Reverse transcriptases and RT-qPCR kits Maxima First Strand cdna Synthesis Kit is optimised for cdna synthesis in 2-step RT-qPCR applications. The Maxima Reverse Transcriptase (RT) is derived by in vitro evolution of M-MuLV RT. It features high thermostability and robustness. The reaction can be completed in 15 minutes. Verso 1-step RT-qPCR Kits are optimised for onestep RT-qPCR applications. An optional RT Enhancer eliminates the need for DNaseI treatment by removing DNA contamination during the reverse transcription step when added to the mixture. Solaris qpcr Gene Expression Assays pre-designed assays for human and mouse genomes Solaris qpcr Gene Expression Assays are gene-specifc probe and primer pairs that utilise Minor Groove Binder (MGB) and Superbase technologies to deliver repeatable and sensitive relative quantification of human and mouse gene expression. Each assay is designed to a consensus sequence that includes all known splice variants of the target gene. You only need one optimal assay for each gene target. Solaris qpcr Gene Expression Master Mixes are optimised master mixes used with Solaris Assays. Solaris RNA Spike Control Kit is designed to verify that reverse transcription and qpcr steps are not inhibited by any components in the RNA sample or purification process. Inhibition can be the cause of unexpected or variable results. Please contact us for further details of our PCR and qpcr products. We offer a comprehensive portfolio of products for the entire qpcr workflow. These include innovative, high-class instruments, reagents and consumables for sample storage, sample preparation, nucleic acid extraction and qpcr analysis as well as for manual and automated liquid handling. For more information, please contact us at info@cometascientific.com Discover our extensive portfolio for the qpcr workflow > > > Sample Storage and Preparation DNA / RNA Extraction DNA / RNA Analysis > > > qpcr Liquid Handling Centrifuges Freezers Refrigerators KingFisher instruments Reagents and consumables for nucleic acid purification Multiskan GO spectrophotometer PikoReal 24 and 96 Reagents, assays and consumables for qpcr Finnpipette pipettes Matrix pipettes Versette liquid handler 361

18 PikoReal Real-Time PCR System - Technical Specifications and Ordering Information Thermal Block Block formats 24-well, 96-well (not interchangeable) Sample volume 0 50 µl (PikoReal 24), 5 20 µl (PikoReal 96) Consumables 24-well and 96-well Piko PCR Plates; for 24-well block also low profile strip tubes and PCR tubes Max heating rate > 5 C/sec Max cooling rate 4.5 C/sec Temperature range C Temperature accuracy ±0.2 C Temperature uniformity ±0.3 C at 95 C, ±0.15 C at 60 C, ±0.2 C at 72 C Heated lid Temperature range Control C Automatic temperature and pressure setting Optics Excitation 5 LEDs Excitation range nm Detection CCD Detection range nm Detection channels: Channel Exitation (nm) Emission (nm) Pre-Calibrated Dyes FAM HEX, Yakima Yellow ROX, Texas Red Cy SYBR Green Multiplex Up to 4 targets Dynamic range 10 logs Sensitivity 1 copy Scan time for 4 multiplexing channels < 10 sec Software Analysis modes Absolute quantification, Relative quantification, Melt curve analysis, Allelic discrimination and High Resolution Melting System Operating systems Windows XP, Windows 7 Communication Ethernet (up to 10 instruments can be operated from a single PC) or USB Power usage 200 W maximum Dimensions (W x D x H) 300 x 230 x 310 mm Weight 10 kg Cat. No. TCR0024 TCR0096 Description PikoReal 24 Real-Time PCR System PikoReal 96 Real-Time PCR System 362 Online:

19 Multidrop Combi Reagent Dispenser The ultimate versatility for bulk reagent dispensing The Multidrop Combi reagent dispenser offers unrivalled levels of performance, versatility and simplicity for pharmaceutical and biotech laboratories. Based on the proven and highly reliable Multidrop range, Multidrop Combi dispenser has been designed for effortless highspeed dispensing of different types of solutions. For high-throughput screening, Multidrop Combi dispenser dispenses into 1536-well plates at nanolitre levels with minimal dead volume. At the other end of the scale, being able to dispense at volumes up to 2500 µl offers biotech researchers a wide range of applications. Add full robotic capability, and the Multidrop Combi dispenser is your answer for problem-free, highthroughput bulk dispensing. Multidrop Combi reagent dispenser offers you : Unmatched volume range precise dispensing over a µl range, giving reproducible assay data Any plate, any well size from 6 to 1536 wells and plate heights of 5 to 50 mm the Multidrop Combi dispenser is perfect for any application Simplest operation the visual icon-based graphic display makes it easy to use and program, even without training Reduced reagent costs low volume dispensing, minimal dead volume and back-flushing minimises re-runs and usage of expensive reagents Unique cassette lifetime traceability Multidrop Combi dispenser, equipped with the SMART option, counts how many times the dispensing cassette is used Increased functionality - easy-to-use FILLit Software provides means to develop and transfer protocols into the instrument, as well as offering an extensive plate selection High-throughput, 24/7 operation full robotic compatibility gives increased throughput, backed up by the reliability of the Multidrop technology proven in use with over 9,000 units installed worldwide Total flexibility From 6 to 1536 wells, the Multidrop Combi dispenser is capable of dispensing into a full range of plates, with automated dispensing-height adjustment for low-profile to deep-well plates, it offers complete flexibility for any dispensing application. The small footprint enables Multidrop Combi dispenser to fit into laminar cabinets and together with the extensive dispensing volumes, versatile column selection and dispensing speed options, the Multidrop Combi dispenser is ideal for all pharmaceutical and biotech researchers. Extensive applications Assay development Primary and secondary screening Compound storage Genomics and proteomics research - PCR setup - Sequencing setup Cell-based assays ELISA assays Wide range of solutions Buffers Reagents Diluents Solvents Viscous solutions - detergents and substrates Cells Beads 363

20 Multidrop Combi Reagent Dispenser Precise dispensing Multidrop Combi dispenser utilises the peristaltic pump technology and unique Multidrop dispensing cassettes to ensure absolute precision across an unrivalled volume range. CV s of <10 % at 0.5 µl will empower drug discovery teams to design new high-throughput assays on 1536-well plates. Similarly, dispensing up to 2,500 µl is ideal for some delicate cell-based assays. Dead volumes are minimised at all settings. A wide range of dispensing speeds is also possible and up to eight different solutions can be dispensed simultaneously. The possibility to adjust different volumes for each individual column enables the user to fill the plate with different dilutions of the same reagent. Overall result? A steady flow of highly reproducible, meaningful data, leading to real increases in productivity. Easy to use Through the simple and visual interface, Multidrop Combi enables users to take full advantage of its technical advances. Descriptive icons make it easy to change all dispensing parameters for customised work such as pump speed, tip height, offset values, dispensing direction, etc. or go straight to pre-set protocols. The users can also store their own protocols in the instrument memory, from which they can be launched in seconds. In all cases, the intuitive interface means that little or no training is required, so productivity is raised. The FILLit Software, which comes with the instrument, increases the functionality of the Multidrop Combi dispenser. Autoclavable dispensing cassettes and reagent backflushing To ensure sterile conditions and avoid cross contamination, the Multidrop Combi dispenser uses 8-channel detachable and autoclavable dispensing cassettes that are standard across the Multidrop range. Cassettes are selected according to the dispense volumes and user preference, and changed in seconds. Back-flushing of tubing and the Multidrop Combi dispenser s negligible dead volumes minimise expensive reagent loss. Together with robotic integration, this ease of use provides hassle-free, low-cost and uninterrupted operation. Daily maintenance is quick due to the easy-clean design of the unit, which allows access under the plate carrier. Full robotic compatibility Multidrop Combi dispenser can increase productivity as a stand-alone instrument. However, when users take advantage of its full robotic compatibility, the real benefits become clear. The dispenser s plate carrier is optimised for easy plate access by robotic arm grippers, linked via either the RS-232 serial or USB port. Furthermore, the RapidStak stacker can be added to create a complete benchtop solution for automated bulk reagent dispensing. The autoclavable Multidrop dispensing cassettes come with different tubing size, and tip material and size. Choose between standard and small tube cassettes, or SMART versions of these, which store dispensing lifetime data. Multidrop Combi s highly visual user interface helps you quickly start dispensing by enabling customised settings. RapidStak TM with Multidrop Combi dispenser creates a complete benchtop solution for automated bulk reagent dispensing Dispensing precision (CV%) with µl. Different volumes of 0.04 M fluorescein was dispensed into black 384-Well Microtitre Microplates and measured at Ex485/Em518 nm. 364 Online:

21 Multidrop Combi Reagent Dispenser - Technical Specifications and Ordering Information Technical Specifications Plate types 6-, 12-, 24-, 48-, 96-,384-, 1536-well plates (plate height 5-50 mm) Dispensing volume range µl Dispensing precision (CV%) Small tube dispensing cassette Standard tube dispensing cassette 0.5 µl CV 10% 5 µl CV 10% 2 µl CV 5% 20 µl CV 1.5% 10 µl CV 3% 100 µl CV 1% >10 µl CV 3% >100 µl CV 1% Dispensing accuracy Small tube dispensing cassette Standard tube dispensing cassette 2 µl ± 10% (typical) 5 µl ± 3% (typical) 10 µl ± 5% (typical), 20 µl ± 2% (typical) > 10 µl ± 5% (typical) 100 µl: ± 1% (typical) > 100 µl ± 1% (typical) Dispensing speed 96-well plate: 10 µl in 3 seconds, 20 µl in 4 s., 100 µl in 10 s. 384-well plate: 1µl in 5 s., 5 µl in 5 s., 10 µl in 6 s., 20µl in 9 s well plate: 1 µl in 14 s., 5 µl in 26 s. Dispensing increments Small tube dispensing cassette: 0.5 µl increments Stand tube dispensing cassette: 5µl increments Dead volume < 1 ml vol range µl < 7 ml vol range > 50 µl Interface Serial RS-232, USB Overall dimensions 220(H) x 355(W) x 330(D) mm 8.6(H) x 14(W) x 12.9(D) in. Weight 9.1 kg (20.1 lbs.) Power requirements V, 50/60 Hz, nominal Ordering information Multidrop Combi, V 50/60 Hz Multidrop Combi with SMART option, V 50/60 Hz Includes cassette lifetime traceability feature FILLit Software for Multidrop Combi Small tube plastic tip dispensing cassette (for volumes µl) Long small tube plastic tip dispensing cassette (for volumes µl) Small tube metal tip dispensing cassette (for volumes µl) Long small tube metal tip dispensing cassette (for volumes µl) Small tube plastic tip dispensing cassette 5-pack Small tube metal tip dispensing cassette 5-pack Standard tube dispensing cassette (for volumes µl) Standard tube dispensing cassette 5-pack Standard tube dispensing cassette 10-pack Long standard tube dispensing cassette (length of tubing cm in 10 cm increments) 365

22 Multidrop 384 and Multidrop DW Reagent Dispensers The Multidrop 384 and Multidrop DW are bulk reagent dispensers providing day to day reliability and precise dispensing results for laboratories needing high-speed plate filling to increase throughput. Multidrop 384 Multidrop DW Detachable 8-channel dispensing cassette Reliable Dispensing Tools The Multidrop 384 and Multidrop DW are known as reliable and robust dispensing tools offering benefits for routine dispensing needs in all laboratories. The reliable operation, combined with excellent dispensing precision, guarantees solid assay data and reproducible results minimising extra work needed. The easy integration with different automated systems, combined with ease of use results in higher productivity. Precise Dispensing into Microplates The Multidrop 384 and DW allow continuous dispensing by using a peristaltic pump and dispensing directly from practically any reagent bottle. Up to 8 different liquids can be dispensed into any number of columns using the 8-channel dispensing cassette. The instruments are ideal for volumes over 20 µl, and Multidrop 384 dispenses volumes µl in 5 µl increments into 96- and 384-well plates. The Multidrop DW allows dispensing of higher volumes and dispenses volumes of µl into standard 96-well plates, DW plates and tubes in 96-well racks. Both Multidrops offer excellent dispensing precision, the CV is less than 1.5% when dispensing 20 µl. Autoclavable Dispensing Cassette Multidrops utilise detachable and autoclavable 8-channel dispensing cassettes with silicone tubing. All reagent lines can be back-flushed into a reagent bottle, minimising the loss of expensive reagents and the dead volume. Cassettes feature wide tubing and a tip diameter which allows reliable dispensing of living cells into microplates. Easy to Use The instruments are easy to use with quick volume selection from the instrument keypad and their compact size fits them comfortably into laminar cabinets as well as into automated systems. Although Multidrops work as stand-alone instruments, they can be easily integrated with robotic platforms via the RS-232 serial port to provide even greater flexibility and higher throughput. Combined with the RapidStak microplate stacker, Multidrops offer an affordable bench top automation solution. Dispensing cassettes with extended tubing length and a special priming vessel are also available for use in automated environments. Multidrop s capability to prepare plates fast, allows laboratories to speed up their dispensing and increase throughput. 366 Online:

23 Multidrop 384 and Multidrop DW Reagent Dispensers - Technical Specifications and Ordering Information Multidrop 384 DW Plate types 96-, 384-well plates 96-well plates 96-deep well plates Tubes in 96 tube rack Dispensing volume range µl µl Dispensing precision 5 µlcv 10% (typical) 20 µl CV 1.5% (typical) 20 µl CV 1.5% (typical) 100 µl CV 1% (typical) 100 µl CV 1% (typical) 900 µl CV 0.5% (typical) Dispensing accuracy 5 µl ±3% (typical) 20 µl ±2% (typical) 20 µl ±2% (typical) 100 µl ±1% (typical) 100 µl ±1% (typical) 900 µl ±1% (typical) Dispensing speed 96-well plate: 96-well plate: 20 µl in 5 s 20 µl in 5 s 50 µl in 8 s 50 µl in 8 s 384-well plate: 300 µl in 26 s 20 µl in 20 s 900 µl in 74 s 50 µl in 25 s Dispensing increments 5 µl increments 5 µl increments Dead volume <7 ml *) <7 ml *) Interface RS-232 RS-232 Dimensions (HxWxD) 155 x 310 x 320 mm 180 x 310 x 320 mm 6.1 x 12.2 x 12.6 in. 7.1 x 12.2) x 12.6 in. Weight 6.2 kg (13.7 lbs.) 6.2 kg (13.7 lbs.) *) Reagent recovery option Ordering Information Cat. No. Description Multidrop 384, V 50/60 Hz 1 ) Multidrop DW, V 50/60 Hz 1 ) 1) Multidrop 384 and DW include one dispensing cassette catalogue number Multidrop Accessories Cat. No. Description Standard tube dispensing cassette Standard tube dispensing cassette 5-pack Standard tube dispensing cassette 10-pack Long standard tube dispensing cassette (length of tubing cm in 10 cm increments) Tubing set for dispensing cassette (40 cm tubing set) Special tubing set for dispensing cassette (length of tubing cm in 10 cm increments) 367

24 Versette Automated Liquid Handler User-Friendly Liquid Handling in a Compact Format The Versette is a compact automated liquid handler that fits a wide range of research laboratories. A choice between 96- or 384-channel pipetting heads and user friendly programming combined with reliable performance makes it an ideal choice for liquid handling needs of most laboratories. Versette TM is designed to perform a variety of liquid handling tasks for a wide range of applications including high-throughput immunoaffinity sample preparation with Mass Spectrometric Immunoassay (MSIA ) pipette tips. It also supports 96/384 plate replication, plate stamping and serial dilution procedures. 96- and 384-channel pipetting heads Versette automated liquid handler is compatible with interchangeable 96- and 384-channel pipetting heads with a total volume range µl. All pipetting heads utilise disposable tips and include RFID tags to self-identify. Consumables for Optimised Performance The 96- and 384-channel pipetting heads utilise D.A.R.T. s (Disposable Automation Research Tips) tips which include a unique surface seal design that ensures accurate and precise pipetting by forming an even seal across all pipetting channels. D.A.R.T. s technology guarantees accurate and precise pipetting results in all applications across the pipetting range. Compact size The compact design of the Versette automated liquid handler allows it to fit on standard laboratory benches and biological hoods. The unit is equipped with a safety shield to ensure maximum user safety. The six position stage uses a unique dual-level design to further minimise space requirements. User-Friendly Programming Options The user can start creating simple pipetting procedures using the visual icon-based onboard graphical display which makes operation simple and easy. For more protocols and advanced editing PC-based ControlMate software is available. With the ControlMate software you can easily create and run a variety of pipetting operations, from repetitive transfers to complex sequences. Wide range of applications The Versette delivers consistent and reliable performance for a variety of applications including plate stamping, plate reformatting, serial dilution and many other applications in low to high-throughput laboratories. The instrument also serves as an immunoaffinity sample preparation platform within our new Mass Spectrometric Immunoassay (MSIA) integrated workflow. The MSIA tips are compatible with the Versette liquid handler where sample binding, wash and elution steps are automated to facilitate cycling of sample and the appropriate buffers through the microcolumn embedded in the pipette tip. The complete walk-away immunoaffinity system offers increased sample throughput while providing high assay reproducibility. 368 Online:

25 Versette Automated Liquid Handler - Technical Specifications and Ordering Information Specifications Volume range Compatible labware Operating system Interface Dimensions (WxDxH) Weight Power requirements µl (depending on the pipetting head type) 96-, 384- well plates with different heights, storage tubes in rack ( ml) Windows RS x55x68 cm (27x22x27 in) base unit with 6 position stage 54.4 kg (120 lbs.) base system (heads / pipetting module not included), 36 kg (80 lbs.) Base (tower), 18.1 kg (40 lbs.) 6 Position Stage, 11.8 kg (26 lbs.) NTC Pipetting Module, 12.7 kg (28 lbs.) Pump Module, 2.7 kg (6 lbs.) Hood V, 50/60 Hz Pipetting accuracy and precision Cat.No. Description Accuracy Precision Channel air displacement pipetting head. Volume µl 2.0% or 0.15 µl 1.5% or 0.10 µl Channel air displacement pipetting head. Volume µl 2.0% or 1.00 µl 1.5% or 0.75 µl Channel air displacement pipetting head. Volume µl 2.0% or 0.50 µl 1.5% or 0.25µl Ordering information Cat.No. Description BS Versette base unit Stage, head housing and pipetting head required for use NTC 96- and 384-channel housing assembly For use with 96- and 384-channel pipetting heads SPS 6-position stage, guarding included PUMP Pump module Optional accessory, used for tip washing / reagent replenishing TTW 96 channel tip wash station, tall Optional accessory TTW 384 channel tip wash station, tall Optional accessory Consumable compatibility (D.A.R.T. s) Pipetting Head Compatible D.A.R.T. s Cat.No. Description Cat. No. Description ch. Volume µl 5586 (ns)* / 5587 (sterile) / 5588 (sterile filtered) 96-ch µl 5506 (ns)* / 5507 (sterile) / 5508 (sterile filtered) 96-ch µl extended length ch. Volume µl 5516 (ns)* / 5517 (sterile) / 5518 (sterile filtered) 96-ch µl 5536 (ns)* / 5537 (sterile) / 5538 (sterile filtered) 96-ch µl extended length 5546 (ns)* / 5547 (sterile) / 5548 (sterile filtered) 96-ch µl extended length, wide bore ch. Volume µl 5326 (ns)* / 5327 (sterile) / 5328 (sterile filtered) 384-ch µl * ns = non-sterile 369

26 Pipettes Maximise research efficiency with consistent, reproducible results. Our complete range of manual and electronic pipetting systems, automated liquid handlers and dispensers, as well as tips for any instrument you have in the lab, provide the confidence you need to accelerate discoveries. So you can handle anything. Finnpipette F1 Pipetters Integrated antimicrobial surface protection prevents contamination Light pipetting forces significantly reduced RSI (Repetitive Stress Injury) risks Set-and-forget pipetting button securely locks volume adjustment Adjustable finger rest Cat. No. Finnpipette F1 Adjustable-Volume Pipettes Finnpipette F µl, Micro Finnpipette F µl, Micro Finnpipette F µl, Micro Finnpipette F µl Finnpipette F µl, Micro Finnpipette F µl Finnpipette F µl, Micro Finnpipette F µl Finnpipette F µl Finnpipette F µl Finnpipette F µl Finnpipette F µl Finnpipette F ml Finnpipette F ml Cat. No. Finnpipette F1 Fixed-Volume Pipettes Finnpipette F1 1 µl, Micro Finnpipette F1 5 µl, Micro Finnpipette F1 10 µl Finnpipette F1 20 µl Finnpipette F1 25 µl Finnpipette F1 50 µl Finnpipette F1 100 µl Finnpipette F1 200 µl Finnpipette F1 250 µl Finnpipette F1 500 µl Finnpipette F µl Finnpipette F µl Finnpipette F µl Finnpipette F µl Finnpipette F µl Cat. No. Finnpipette F1 Multichannel Pipettes Finnpipette F1 8-channel 1-10 µl, Micro Finnpipette F1 8-channel 5-50 µl Finnpipette F1 8-channel µl Finnpipette F1 8-channel µl Finnpipette F1 12-channel 1-10 µl, Micro Finnpipette F1 12-channel 5-50 µl Finnpipette F1 12-channel µl Finnpipette F1 12-channel µl Finnpipette F1 16-channel 1-10 µl, Micro Finnpipette F1 16-channel 5-50 µl, Micro 370 Online:

27 Pipettes Breakthrough ClipTip Technology You will feel the tips lock firmly in place with a light touch. It s time to change the way you pipette, with the ClipTip pipetting system. Finnpipette F2 Pipetters Advanced Volume Gearing mechanism (AVG) improves accuracy and precision Double-action pipetting button for easy and light volume setting Easy to open for piston cleaning and service Fully autoclavable Cat. No. Finnpipette F2 Adjustable-Volume Pipettes Finnpipette F µl, Micro Finnpipette F µl, Micro Finnpipette F µl, Micro Finnpipette F µl Finnpipette F µl, Micro Finnpipette F µl Finnpipette F µl,micro Finnpipette F µl Finnpipette F µl Finnpipette F µl Finnpipette F µl Finnpipette F ml Finnpipette F ml Cat. No. Finnpipette F2 Fixed Volume Pipettes Finnpipette F2 1 µl, Micro Finnpipette F2 5 µl, Micro Finnpipette F2 10 µl Finnpipette F2 20 µl Finnpipette F2 25 µl Finnpipette F2 50 µl Finnpipette F2 100 µl Finnpipette F2 200 µl Finnpipette F2 250 µl Finnpipette F2 500 µl Finnpipette F µl Finnpipette F µl Finnpipette F µl Finnpipette F µl Finnpipette F2 Volume µl Cat. No. Finnpipette F2 Multichannel Pipettes Finnpipette F2 8-channel 1-10 µl, Micro Finnpipette F2 8-channel 5-50 µl Finnpipette F2 8-channel µl Finnpipette F2 8-channel µl Finnpipette F2 12-channel 1-10 µl, Micro Finnpipette F2 12-channel 5-50 µl Finnpipette F2 12-channel µl Finnpipette F2 12-channel µl Finnpipette F2 16-channel 1-10 µl, Micro Finnpipette F2 16-channel 5-50 µl, Micro Note: Please contact us for details of our electronic pipettes. Our broad selection of pipette tips perform with high precision and accuracy by providing optimal liquid handling results. Please contact us for further details. 371

28 RapidStak Microplate Stacker The RapidStak TM is the fastest, most flexible microplate stacker in its class. It can be set up in minutes, providing walk-away time and relief from monotonous tasks. Dual-instrument loading allows plates to be quickly delivered and processed using two instruments simultaneously. And it can be easily upgraded to triple the capacity, making RapidStak the most versatile and productive stacker on the market. Automate your instruments for unattended operation using the fastest, most flexible stacker in its class. The Advantages Stack Up Laboratory automation frees you to concentrate on more important tasks. The RapidStak microplate stacker maximises that walk-away time. Just fill the stacker with plates, start it, and walk away. The RapidStak model holds 2 Staks, for a capacity of 50 plates. Its larger version, the RapidStak 2x, holds 4 Staks handling up to 150 plates, giving you even greater walk-away time. The RapidStak Difference The fastest pick-and-place microplate stacking on the market As little as 6 seconds per plate load/unload Up to 300 plates per hour throughput Easy upgrade from base unit to 2x model for triple the capacity Rapid, easy setup in the field for least amount of instrument downtime Quickly and seamlessly interchange our own or third-party instruments on one stacker Does not interfere with the standalone use of the instruments Two Instrument Automation The RapidStak now offers even greater versatility with the ability to automate virtually any two instruments simultaneously with the addition of the POLARA RS software. The system seamlessly integrates with almost any of our own or third party instruments, so you can perform an endless range of applications and assays. The Reliable Choice The RapidStak unit is built upon our heritage of robotics leadership and systems integration expertise, which generate the highest value in your research applications. The RapidStak is as tough and reliable as our proven automation platforms. 372 Online:

29 RapidStak Microplate Stacker - Technical Specifications and Ordering Information Models F01350 RapidStak (2 Staks) F01351 RapidStak 2x (4 Staks) F01489 Shortened RapidStak (2 Staks) F01490 Shortened RapidStak (4 Staks) Staks (assume 15mm plates) F plate stak F plate stak F plate stak Compatible Microplate Types 6-22mm, SBS conforming Speed and Throughput 6 second per plate delivery/retrieval Up to 300 plates per hour 5 seconds restacking per plate (approx.) Plate Capacity (10/15 mm plates) RapidStak 50 / 75 plates RapidStak 2x 150 / 225 plates (Above capacities still allow for destination Stak) Safety Conformance CE, CSA, UL Power Requirements volts, autoswitching Upgrades Field upgradeable to 2x (4-Stak model) 3rd party instruments integration Programming Toolkits Offset telescoping arm Polara RS, PC scheduling software Microplate lid handling Dual instrument loading Custom Stak sizes Unique Technologies 2-stage cam for plate stack/unstack Bidirectional, telescoping arm for two instrument loading Buffer Nest to pre-queue plates Choreography for simultaneous plate handling RapidStak RapidStak 2x RapidStak Integrated with Multidrop Combi and ALPS