MPure-12. Bringing You Beyond Expectations. Automated Nucleic Acid Purification. Explore New Possibilities in Nucleic Acid Purification!

Size: px

Start display at page:

Download "MPure-12. Bringing You Beyond Expectations. Automated Nucleic Acid Purification. Explore New Possibilities in Nucleic Acid Purification!"

Annice Fleming
5 years ago
Views:

1 Bringing You Beyond Expectations Superior Yield & Results. Reliable Performance. Simplicity. Flexibility. Purity. Convenience. Affordability! Explore New Possibilities in Nucleic Acid Purification! E ice BEST CHOIC Be Automated Nucleic Acid Purification st Quality Be st rv MPure-12 TM Pric e Bes e ts

MPure-12 is a bench-top automated system for rapid purification of nucleic acids from a wide variety of bio-specimens using magnetic bead separation technology.

affordable price. Step away from the norm and experience the difference in nucleic acid purification.

2 MPure-12 is a bench-top automated system for rapid purification of nucleic acids from a wide variety of bio-specimens using magnetic bead separation technology. The MPure has a uniquely designed magnetic bead processing chamber and is fully integrated with easy to use pre-packaged reagent kits offering superior yield and purity of nucleic acids at an affordable price. Step away from the norm and experience the difference in nucleic acid purification. Benefits of Automation: Fully automated and integrated platform that offers cost and time savings Purify nucleic acids of the highest quality and yield for downstream applications Enjoy the flexibility and simplicity to process 1-12 samples from a wide range of bio-specimens in a single cycle Minimum cross contamination of samples based on the unique design of the platform Minimized nucleic acid loss and degradation Reproducibility, lot-to-lot consistency, scalability, ease-of-use and convenience Closed system that offers increased safety and efficiency Best Purification System at an Affordable Price

3 The MPure-12 System employs an advanced magnetic bead separation technology that enables rapid and efficient purification of nucleic acids. This process includes four main steps: Lysis, binding, washing and elution. Purifying the nucleic acids with the MPure-12 System takes only 35 to 70 minutes depending on the selected protocol and kit. The figure shown below illustrates the complete steps involved in the purification of nucleic acid. Shortest Run Time: minutes MP Biomedicals US:

Polygon Reaction Chamber Designed for Increased Yield and Minimized Cross Contamination

Increased yield and purity Magnet is positioned for clog free removal of sample Liquid

and unidirectional movement of robotic arm prevents cross contamination Increased pitch

consumable per sample UV Lamp Facilitates Decontamination Between Runs Built-in UV Lamp

4 Polygon Reaction Chamber Designed for Increased Yield and Minimized Cross Contamination Polygon design minimizes cross contamination and increases efficiency of lysis Increased yield and purity Magnet is positioned for clog free removal of sample Liquid Handling Platform Unilateral Arm Movement Minimizes Cross Contamination Synchronized and unidirectional movement of robotic arm prevents cross contamination Increased pitch distance between reagent cartridge and sample tubes Use of one set of plastic consumable per sample UV Lamp Facilitates Decontamination Between Runs Built-in UV Lamp enables decontamination and guarantees purity of the sample Use the UV lamp before and after the extraction

5 Automation Features Unique Design of Magnetic Bead Chamber Pre-filled Reagent Cartridges Barcoded ID Cards & Reader Reagent Kits Single Harmonized movement for Sample Handling UV Lamp Throughput Benefits Convenience, Ease-of-use, Cost & Time Savings, Hands-Free Approach. Consistent Results, Reproducibility, Avoid Carry Over & Cross Contamination, High Quality Samples, Better Yield, Better Purification Efficiency. Lot-to-Lot Consistency of Results, Convenience, Simplicity, Better Yield, Avoid Cross Contamination, Standardization, Cost Savings. Preset Protocols, Easy Selection & Tracking of Protocols, Convenience. Wide Array (15 Kits), Cost & Time Savings, Lowest Price, Reproducibility, High Yield, Consistency, All Inclusive. Avoids Cross Contamination during Sample Processing and Handling. Decontamination of Processing Chamber between runs. Process 1 to 12 samples in <45 minutes, Flexibility, Cost & Time Savings. Feature MPure12 Product Q Product R # of Samples/run 1 to 12 1 to 14 1 to 8 Process Time < 45 minutes Yes No No Magnetic Beads/Pillar Magnetic Beads Magnetic Beads Magnetic Beads Unique Design of Magnetic Chamber to Minimize cross Contamination Yes No No Protocol ID Card Needed No Yes Yes Barcode Reader - Easy Protocol Set-up Yes No No UV Lamp - Decontamination Yes No Yes Instrument Price < $18,000 Yes No No Cost/Prep < $5.20 each Yes No No MP Biomedicals US:

Sample cartridge offers flexibility to handle 1 to

individual cartridge prevents cross contamination

Barcode Reader Quick Set-up of Protocols Barcode

protocol cards Set up run in 3 steps: 1) select

Convenient, fail-proof start and ease-of-use.

Convenient for sample loading and collection of

Maintenance Worry Free Operation No O-Rings in the

Port for uploading protocols and software updates

6 Sample cartridge offers flexibility to handle 1 to 12 samples Minimizes wastage of reagents Use of individual cartridge prevents cross contamination and saves cost Convenience and Flexibility 1 sample Barcode Reader Quick Set-up of Protocols Barcode reader facilitates scanning the pre-programmed protocol cards Set up run in 3 steps: 1) select protocol, 2) set volume, and 3) set elution volume Convenient, fail-proof start and ease-of-use. Removable rack for sample and elution tubes Convenient for sample loading and collection of purified nucleic acid Purified nucleic acids auto collected into 1.5 ml elution tube 6 samples 12 samples Minimum Maintenance Worry Free Operation No O-Rings in the Piston Head Modular design for easy maintenance USB Port for uploading protocols and software updates 18-month warranty on instrument Select the extraction protocol and set the sample and elution volumes with three quick scans.

7 System Specifications Model MPure - 12 Cat. No Dimensions Weight Display Operating Temperature Operating Relative Humidity Power Supply Extraction Method Processing capability Processing Time Sample Volume Elution Volume Hardware Protocol Safety 48 cm W x 70 cm D x 52 cm H 43 kgs LCD (20 characters x 4 lines) 15 to 30 o C 30 to 80% (non-condensing) V/ 50/60Hz Magnetic particle separation technology 1 to 12 samples/run 30 to 50 minutes 100 to1000 μl 50 to300 μl Integrated micro-processor, no external PC required On board, preinstalled protocols CE IVD The MPure-12 System is a standalone benchtop system; no PC is needed. The MPure-12 extracts nucleic acids in three simple steps: Load, Run and Obtain. The MPure-12 extracts samples of any type or quantity. For tough samples, lyse first with our FastPrep-24 5G, a standalone tissue homogenizer. The FastPrep-24 5G is capable of lysing any sample type in 40 seconds or less, while providing a high yield and purity of the analyte. The FastPrep-24 5G is available with a variety of sample holders that allow versatility in processing sample tubes of 2 to 50 ml, or ambient or cryogenic samples. Difficult to lyse samples are easily processed by lysing with the FastPrep-24 5G followed by the extraction of nucleic acids with the MPure-12 System. Truly Walk Away Automation LOAD RUN OBTAIN Load samples, reagent cartridges and consumables Select protocol with a quick barcode scan. Instrument automates the rest At the end of the run, purified necleic acids are auto collected High Quality, Reliable, Consistent Results Offering you the Peace of Mind to make the Final Decision MP Biomedicals US:

Flavor of Reagent Kits Widest Possible Choice to meet your Research Needs 15 different reagent kits for the purification of DNA and RNA Purify DNA from Blood, Tissues,

preps and pre-packaged for a fully automated set-up and run Scalability: Sample Volume ranges from 50 to 1000 μl and the Elution Volume ranges from 100 to 200 μl Convenient

MPure Kits contain everything needed for sample purification: Pre-filled reagent cartridges, reaction chambers, sample tubes, elution tubes, disposable pipette tips, etc.

Reagent cartridges are pre-measured and film-sealed to avoid contamination; the MPure-12 System automatically pierces this film before moving the reagent to the sample tube,

8 Flavor of Reagent Kits Widest Possible Choice to meet your Research Needs 15 different reagent kits for the purification of DNA and RNA Purify DNA from Blood, Tissues, Cultured Cells, FFPE, Bacteria, Plant and Forensic samples Purify Viral/Pathogenic DNA and Total RNA from Serum or Plasma as well as from Swabs Each kit is designed for 48 preps and pre-packaged for a fully automated set-up and run Scalability: Sample Volume ranges from 50 to 1000 μl and the Elution Volume ranges from 100 to 200 μl Convenient sealed reagent cartridges MP Biomedicals offers 16 different MPure-12 Kits that allow for the purification of DNA and RNA from a wide range of bio-specimens. MPure Kits contain everything needed for sample purification: Pre-filled reagent cartridges, reaction chambers, sample tubes, elution tubes, disposable pipette tips, etc. MPure-12 pre-filled reagent cartridges are designed to increase analyte yield and data quality while decreasing contamination risk and cost. Reagent cartridges are pre-measured and film-sealed to avoid contamination; the MPure-12 System automatically pierces this film before moving the reagent to the sample tube, eliminating operator handling errors. Each cartridge contains reagents for one sample and cartridges may be run individually, allowing flexibility in the number of samples processed (from 1 to 12) with no unnecessary waste of reagents.

MPure-12 Offers Superior Performance Compared to the Competition The purity of the Genomic DNA is higher with low variation Yield of gdna is comparable or higher with the lowest variation Results of

76 2 1.92 4.93 1.86 6.57 1.85 5.10 3 1.83 5.16 1.85 6.44 1.71 3.98 4 1.89 3.93 1.82 4.27 2.04 4.14 5 1.96 4.54 1.82 3.77 1.87 4.84 6 2.05 5.18 1.87 5.27 1.81 4.60 7 1.96 4.31 1.90 4.84 1.83 3.65 8 2.

9 MPure-12 Offers Superior Performance Compared to the Competition The purity of the Genomic DNA is higher with low variation Yield of gdna is comparable or higher with the lowest variation Results of gdna Purification from 12 Whole Blood Samples MPure-12 Product Q Product R Purity Yield Purity Yield Purity Yield Sample # A260/A280 (μg) A260/A280 (μg) A260/A280 (μg) Mean SD * Blood samples were donated from 12 healthy volunteers ** 200 μl input volume of EDTA-whole blood samples were used. *** The purity and yi8eld of extracted DNA was measured by Nanodrop 2000C Integrity - Reproducibility - The DNA was isolated in replicates of 12 from 200 μl human whole blood taken from two different donors. Elution volume was set to 100 μl. Integrity of DNA was shown by subjecting each eluate to TAE agarose gel electrophoresis together with a suitable molecular weight marker. All samples exhibit single band with molecular weight > 40 kb with no smear. The DNA was isolated by MPure-12 blood DNA extraction kit from twenty whole blood samples. The β-globin gene was detected by real-time qpcr. This data shows ultra high stability and reproducibility of MPure-12 s nucleic acid purification capability. MP Biomedicals US: TAE agarose gel electrophore sis of DNA isolated in replicates of 12 from 200 μl human whole blood taken from two different donors. Argarose Gel Analysis of PCR products. Electrophoresis image shows high consistency and reproducibility on extraction results. β-globin gene was detected by real-time qpcr following isolation with MPure-12 blood DNA kit.

Stability - The DNA was extracted from whole blood by MPure-12 Blood DNA extraction kit 200. Real-time qpcr detection of the β-globin (A) and spiked parvovirus B-19 DNA (A,B).

Sensitivity - Whole blood (in EDTA collection tube) was spiked with serial-dilution of human Parvo B19 Virus (in the range of 5-500,000 IU/L).

10 Stability - The DNA was extracted from whole blood by MPure-12 Blood DNA extraction kit 200. Real-time qpcr detection of the β-globin (A) and spiked parvovirus B-19 DNA (A,B). No significant influences on samples with different anticoagulants and storage conditions. Sensitivity - Whole blood (in EDTA collection tube) was spiked with serial-dilution of human Parvo B19 Virus (in the range of 5-500,000 IU/L). 200 µl samples were extracted and eluted in 100 µl volume. 10 µl of eluate was used for real-time PCR reaction by RealStar Parvovirus B19 PCR kit 1.0. As little as 5 IU spiked (about 1 IU in PCR reaction) sample can be detected, proving the excellent sensitivity and linearity of isolation. Using whole blood (in EDTA collection tube) spiked with serial-diluted human Parvo B19 Virus (in range of IU/l). 10ml eluate was used for real-time PCR reaction by RealStar Par vovirus B19 PCR kit 1.0. Real-time qpcr detec tion of the β-globin (A) and spiked par vovirus B-19 DNA (A,B). Whole blood DNA isolated with MPure-12 kit. Scalability - The DNA was isolated from different whole blood sam ples (WBCs count range x 10 3 cells/μl). DNA yield (measured by Nanodrop 2000) shows excellent scalability in different volumes (100, 200, 300 and 400 μl) of extraction and WBCs count of the samples. The DNA yield shows excellent scalability in different volume (100, 200, 300 and 400 μl) of extraction and WBCs count of the samples.

11 Everything you need for Automated Purification of Nucleic Acids at an Affordable price. Cat. No. Product Name Description Price MPure-12 Nucleic Acid Purification System Fully automated platform for isolation of Nucleic Acid (DNA, RNA) samples Inquire MPure Blood DNA Extraction Kit 200 Purification of Genomic DNA from mammalian whole blood, peripheral blood mononuclear cells, buffy coat - 200uL $250/Kit/48 Preps MPure Blood DNA Extraction Kit 1200 Purification of Genomic DNA from mammalian whole blood, peripheral blood mononuclear cells, buffy coat ul $425/Kit/48 Preps MPure Viral Nucleic Acid Extraction Kit Purification of viral nucleic acids from plasma, serum or cell-free body fluids $250/Kit/48 Preps MPure Tissue DNA Extraction Kit Purification of Genomic DNA from variety of animal tissues, swab and blood stain $250/Kit/48 Preps MPure Cultured Cell DNA Extraction Kit Purification of Genomic DNA from cultured cell $250/Kit/48 Preps MPure Bacterial DNA Extraction Kit Purification of Genomic DNA from bacteria $250/Kit/48 Preps MPure HPV DNA Extraction Kit for Swab Sample Purification of HPV DNA from swab sample $250/Kit/48 Preps MPure TB DNA Extraction Kit Purification of Mycobacterium Tuberculosis DNA from sputum, pulmonary and cultured samples $250/Kit/48 Preps MPure FFPE DNA Extraction Kit Purification of Genomic DNA from formalin fixed, paraffin-embedded tissue (FFPE) samples $250/Kit/48 Preps MPure Forensic DNA Extraction Kit Purification of Genomic DNA from a wide range of forensic and human identity samples, dried blood, bone, swabs and filters $250/Kit/48 Preps MPure Viral/Pathogen Nucleic Acids Extraction Kit B Purification of viral DNA/RNA and pathogen DNA from swab samples $250/Kit/48 Preps MPure Viral RNA Extraction Kit Purification of viral RNA from plasma or serum $250/Kit/48 Preps MPure Plant DNA Extraction Kit Purification of Genomic DNA from plant samples $250/Kit/48 Preps MPure Total RNA Extraction Kit Purification of total RNA from a variety of sample types $250/Kit/48 Preps MPure Viral Nucleic Acid Extraction Kit 800 Purification of viral nucleic acids from plasma, serum or cell-free body fluids $250/Kit/48 Preps MPurify your Nucleic Acid: Obtain Superior Yield, Exceptional Purity, and Automated Performance in a Flexible, Convenient and Affordable Manner! MP Biomedicals US:

12 MPure-12 TM MPurify your Nucleic Acid: Obtain Superior Yield, Exceptional Purity, and Automated Performance in a Flexible, Convenient and Affordable Manner! MP Biomedicals US:

MagCore Automated Nucleic Acid Extractor Overview

MagCore Automated Nucleic Acid Extractor Overview Cost -Effective Cost-Effective MagCore HF16 New Generation MagCore HF16 Plus New! Spectrophotometer Built-in MagCore Super New! Economical and Space Saving