INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA COURSE OUTLINE

Transcription

1 بسم االله الرحمن الرحيم INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA COURSE OUTLINE Kulliyyah Department Engineering Biotechnology Engineering Programme B. Eng ( Biochemical Biotechnology) Course Title Biotechnology Engineering Lab II (THERMAL SCIENCE EXPERIMENTS) Course Code BTE 2511 Status Core Level 2 Credit Hours 1 Contact Hours 3 Pre-requisites Nil (if any) Co-requisites BTE 2450 (if any) Teaching Practical Methodology Method of Evaluation LO Method % 1 12 Lab report 30% 2 10 Pre-lab/Quizzes 10% 4 12 Performance in laboratory 10% TOTAL 50% * Note: This evaluation method only applies to Thermal Science Experiments. Instructor(s) Dr. Mohamed Elwathig Saeed Mirghani Dr. Ahmad Tariq Jameel Sr. Nor Fadhillah Bt Mohamed Azmin Semester All semesters Offered

2 Course Objectives Learning Outcome The objectives of this course are to: 1. Elucidate discussion of the underlying theories associated with techniques. 2. Provide a foundation for advanced research. 3. To understand theories by analyzing the experimental data (learning by doing system) 4. Study cell cycle and cell division of eucaryotic cell and karyotype of human chromosomes 5. Be able to isolate, purify and analyze DNA from different samples Upon completion of this course, students should be able to: 1. Understand and appreciate the Self-confidence in applying the theories into engineering system 2. Understand and appreciate the actual situation that the experimental data is not all the time matches the theoretical values 3. Understand and appreciate the heat capacity of gases at constant volume C V and at constant pressure C p 4. Understand and appreciate the effect of Joule Thomson coefficient on different types of gases 5. Measure the vapour pressure, heat of vaporization and poling point of water 6. Calculate energy taken up and released, also the volume concentration in the circuit and the volumetric efficiency of the compressor. 7. Understand the mechanism and the dynamics of dye binding to protein ovalbumin. 8. Apply separations, purification and drying of α-lactalbumin, one of the major proteins found in milk, by Sephadex gel filtration or affinity chromatography 9. Apply kinetic analysis of enzymes 10. Know the stages of cell cycle and cell division 11. Understand the concepts for DNA isolations from different samples 12. Apply procedures for DNA purifications from different samples Course Synopsis Thermodynamics: Heat Capacity of Gases, Joule-Thomson Effect, Heat of Formation for CO 2 and CO (Hess Law), Thermal and Electrical Conductivity of Metals, Vapour Pressure of Water at High Temperature and Heat Pump. Biochemical kinetics: Protein-Ligand Interaction, Chromatography, Kinetic Analysis of Enzyme and Light Absorption. Emphasis on DNA purification and estimations procedures from different samples.

3 Course Outlines / Contents Week Topics Reading Assignment Experiment 1: Vapour pressure of Water at High Temperature To measure the vapour pressure of water as a function of temperature. To calculate the heat of vaporisation at various temperatures from the values measured. To determine boiling point at normal Experiment 1 pressure by extrapolation. Experiment 2: Heat Capacity of Gases Determine the molar heat capacities of air at constant volume C V and at constant pressure C p. Experiment 3: Joule-Thomson Effect Determination of the Joule-Thomson coefficient of CO 2 and N 2. Experiment 4: Thermal and Electrical Conductivity of Metals (3) ABD + C To determine the thermal conductivity of copper and aluminium is determined in a constant temperature gradient from the calorimetrically measured heat flow. To test the electrical conductivity of copper and aluminium is determined, and the Wiedmann-Franz law. Experiment 5: Heat Pump 1. Water heat pump: To measure pressure and temperature in the circuit and in the water reservoirs on the condenser side and the vaporizer side alternately. To calculate energy taken up and released, also the volume concentration in the circuit and the volumetric efficiency of the compressor. 2. Air-water heat pump: To measure vaporizer temperature and water bath temperature on the condenser side under different operating conditions on the vaporizer side, with stream of cold air, hot air and without blower. Experiment 2 Experiment 3 Experiment 4 Experiment 5

4 Experiment 7: Protein-Ligand Interaction :Binding of Coomassie Blue Dye to Ovalbumin. The dynamics of dye binding to protein ovalbumin will be investigated in order to gain understand of the physical and chemical nature of ligand-macromolecule interactions Experiment 8: Chromatography: Purification and Characterization of α- Lactalbumin, a Milk Protein Purification of α-lactalbumin, one of the major proteins found in milk, by Sephadex gel filtration or affinity chromatography. Characterization of it by UV spectroscopy Experiment 9: Kinetic Analysis of Enzyme: Kinetic Analysis of an Enzyme, Tyrosinase. The tyrosinase activity can be assayed by monitoring the oxidation of 3,4- dihydroxyphenylalanine (dopa) to the red colour dopachrome. The kinetic parameters K m and V max will be evaluated using Lineweaver-Burk or direct linear plots. Inhibition of tyrosinase will also be studied. Finally, two stereoisomers, L-dopa and D- dopa, will be tested and compared as substrates. Experiment 10: Light Absorption: Cholesterol (Total and HDL) and Uric Acid Content of Blood Serum: Enzymes as Reagents in Clinical Chemistry. Two analyses that are common in the clinical laboratory are the measurements of serum cholesterol and uric acid. Cholesterol is measured by coupling the enzymecatalyzed oxidation of cholesterol (generation of H 2 O 2 ) to the peroxidasecatalyzed formation of chromogen. Uricasecatalyzed oxidation of uric acid is basic of urate determination. Experiment 7 Experiment 8 Experiment 9 Experiment 10

5 Cell Division and cell cycle: stages of cell reproduction, their major events, and their sequence. The separation of chromosomes during mitosis and meiosis. Recognition mitotic phases. Staining the chromosomes of onion root tips. Karyotype analysis: differentiation and identification of human chromosomes. Matching up chromosome into homologous pair. Isolation of chromosomal DNA from bacteria Purification and quantification of DNA samples Isolation and purification of DNA from blood sample Agarose gel electrophoresis of DNA from bacteria and blood ences Required: Lab Manual Recommended: Gerald, K. (2003). Cell and Molecular Biology: Concept and Experiments, John Wiley & Sons, Inc. Sambrook and Russell (2001). Molecular cloning a laboratory Manual, Cold spring Harbor Laboratory, Cold Spring Harbor. Proposed Start Date (Semester) Batch of Students to be Affected Semester I, 2007/2008 Semester I, 2005/2006 onwards Prepared by: Checked by: Approved by: (Dr Muataz & Dr. M. Elwathig) (Prof. Suleyman A. Muyibi) (Prof. Ahmad F. Ismail)

6 Course Code: BTE 2511 COURSE NAME: BIOTECHNOLOGY ENGINEERING LAB II NO. Course Learning Outcome COURSE ASSESSMENT MATRIX: BTE 2511 Outcome 1 B.Eng. (Biochemical- Biotechnology) Programme Learning Outcomes 1. Understand and appreciate the Self-confidence in applying the theories into engineering system 2. Understand and appreciate the actual situation that the experimental data is not all the time matches the theoretical values 3. Understand and appreciate the heat capacity of gases at constant volume C V and at constant pressure C p 4. Understand and appreciate the effect of Joule Thomson coefficient on different types of gases 5. Measure the vapour pressure, heat of vaporization and poling point of water Calculate energy taken up and released, also the volume concentration in the circuit and the volumetric efficiency of the compressor. 7. Understand the mechanism and the dynamics of dye binding to protein ovalbumin Apply separations, purification and drying of α-lactalbumin, one of the major proteins found in milk, by Sephadex gel filtration or affinity chromatography 9. Apply kinetic analysis of enzymes Know the stages of cell cycle and cell division Understand the concepts for DNA isolations from different samples Apply procedures for DNA purifications from different samples Index of Biochemical-Biotechnology Engineering Programme Learning Outcomes 1. The ability to acquire and apply knowledge of Mathematics, science and engineering fundamentals. 2. To have acquired a broad based education necessary to understand the impact of engineering solutions in a global and societal context. 3. The ability to have in depth understanding and technical competency in Biochemical- Biotechnology Engineering. 4. The ability to undertake problem identification, formulation and solution. 5. The ability to design a system, component or process for operational performance. 6. The ability to design and conduct experiments as well as to analyze and interpret data. 7. The ability to understand the principles of sustainable design and development. 8. The ability to effectively communicate orally, in writing and using multimedia tools. 9. The ability to function effectively as an individual and in group with the capacity to be a leader or manager as well as an effective team leader member. 10. The ability to recognize the need for life long learning and posses the ability to pursue independent learning for professional development. 11. The ability to understand the social, cultural, global and environmental responsibilities of a professional engineer and the need foe sustainable development. 12. The ability to understand and commit to professional and ethical responsibilities. 13. The ability to understand the expectations of an engineer who practices in an industrial or governmental organization. Outcome 2 Outcome 3 Outcome 4 Outcome 5 Outcome 6 Outcome 7 Outcome 8 Outcome 9 Outcome 10 Outcome 11 Outcome 12 Outcome 13