ARG-ANNOT database consists of a single file covering nucleotide sequences (1689 sequences) in FASTA format from all antibiotics classes (1-10).

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1 S1: Supplementary ARG-ANNOT database link file:. Please go to the link to download the ARG-ANNOT database. (either click the link or copy the link and open in browser) ARG-ANNOT database consists of a single file covering nucleotide sequences (1689 sequences) in FASTA format from all antibiotics classes (1-10). Reference List 1. Scaria J, Chandramouli U, Verma SK Antibiotic Resistance Genes Online (ARGO): a Database on vancomycin and beta-lactam resistance genes. Bioinformation 1: Zhou CE, Smith J, Lam M, Zemla A, Dyer MD, Slezak T MvirDB--a microbial database of protein toxins, virulence factors and antibiotic resistance genes for bio-defence applications. Nucleic Acids Res 35: D391-D Liu B, Pop M ARDB--Antibiotic Resistance Genes Database. Nucleic Acids Res 37: D443-D Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O, Aarestrup FM, Larsen MV Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother. 5. Ramirez MS, Tolmasky ME Aminoglycoside modifying enzymes. Drug Resist Updat 13: Zou LK, Wang HN, Zeng B, Zhang AY, Li JN, Li XT, Tian GB, Wei K, Zhou YS, Xu CW, Yang ZR Phenotypic and genotypic characterization of beta-lactam resistance in Klebsiella pneumoniae isolated from swine. Vet Microbiol 149:

2 7. Van Hoek AH, Mevius D, Guerra B, Mullany P, Roberts AP, Aarts HJ Acquired antibiotic resistance genes: an overview. Front Microbiol 2: clinic website)

3 S2. Supplementery tutorial for BioEdit BioEdit (1) uses the same BLAST program as the NCBI Internet service. Minor differences are due to local processing power and the obvious lack of links to other NCBI databases. Results obtained using a BioEdit BLAST search are directly comparable with results obtained from the NCBI under similar conditions. Things needed to create local database are: Sequence file in FASTA BioEdit plateform. A)- Following steps are needed to set up a local BLAST database with BioEdit 1. Unzip functional copy of BioEdit on a desktop PC from the following link bioedit.html) 2. Follow the instruction to set up BioEdit on the PC. 3. Download the ARG-ANNOT sequence file from the link and the same sequence file would be used to create reference database. 4. For creating local database select accessory application> blast> create database> local nucleotide/protein database. 5. Navigate to the FASTA file of reference sequences and select Open BioEdit will create the files necessary for a local BLAST database, based on the sequences 6. The file will save to the Location of BioEdit in hard drive:\bioedit\database folder as default installation ARGANNOT ARGANNOT.nhr ARGANNOT.nin

4 ARGANNOT.nsq B)- For Blast analysis open the BioEdit window and follow the steps below: 1. BioEdit >Accessory Application>Blast> Local Blast> select blast type (blastn,tblastn, blastp and blastx)> select nucleotide database/protein database> upload or paste query sequences in the dialogue box and hit the search button after selecting the e-value and format for the blast. 2. Selecting Do Search produces a text file with much of the information of an Internet BLAST report. 3. Bidet has an option to filter the search by selecting a different e - value, limit the search by selecting the number of hits to show and number of alignments to display and there is an additional option (go to additional parameter -n for global alignment, - m9 for tabular with details and -m8 tabular form without details). 4. There is a tremendous amount of redundancy in the BLAST output, reflecting many variants of each gene and to reduce it we can use Microsoft XL sheet, in which the blast output (-m8 tabular form without details) can be pasted and filtered using different XL functions. Reference List 1. Hall T BioEdit: Biological sequence alignment editor for Win95/98/NT/2K /XP. Nucleic Acids Symp Ser. 22,

5 Supplementary Table S3: Comparative genome analysis for ARG in diffrent strains of Acinetobacter baumannii using ARG-ANNOT and Resfinder (1) (AGly- Aminoglycosides, Bla-β-lactamase, MLS-Macrolide Lincosamide StreptograminB, Phe-Phenicol, Rif-Rifampicin, Sul-Sulphonamide, Tet-Tetracycline, Tmt- Trimethoprime,Dfr-Trimethoprim, ARG-ARGANNOT, RF-Resfinder). Genomes Time (min) AGly Bla MLS Phe Rif Sul AB ARG 3.3 Aac3 -Ia, Aac6 -IL, StrA, 2xStrB OXA-109, PER-1, 2xBLA, BlaZn, BlaMt Sul-I, Sul-II RF 2.31 Aac3 -Ia, Aac6 -IL,StrA, StrB OXA-109, PER Sul-I, Sul-II AB17978 ARG OXA-180, 2xBLA, BlaZn, BlaMt Sul-II RF OXA Sul-II AB0057 ARG 3.39 Aac3 -Ia, 2xAadA1, Aph3 -Ia TEM-1, OXA-23, 69, 196, 2xBLA, BlaZn, BlaMt -- CatA1 -- 2xSul-I TetA RF 2.29 Aac3 -Ia, AadA1, Aph3 -Ia, OXA-23, TEM-1 -- CatA1 -- Sul-I TetA -- 8 ABACICU ARG 3.16 Aac6 -Ib OXA-18,53, 66, 2xBLA, BlaZn, BlaMt Sul-I RF 2.29 Aac6 -Ib OXA Sul-I ABMDRTJ ARG 3.37 Aac3 -Ia, Aac6 -Ib, AadA1, AadA15, ArmA, StrA, StrB OXA-66, 2xBLA,BlaZn, BlaMt MsrE, MphE Sul-I, Sul-II TetB RF 2.2 Aac3 -Ia, Aac6 -Ib, AadA1, AadA15, ArmA, StrA, StrB OXA-66 MsrE, MphE -- Sul-I, Sul-II TetB ABMDRZJ06 ARG 3.36 Aac6 -Ib, AadA1b, StrA, StrB OXA-23,66, 2xBLA, BlaZn, BlaMt -- CatB3, CatB8 -- Sul-I Tet-B RF 2.26 Aac6 -Ib, StrA, StrB OXA xCatB3 -- Sul-I Tet-B -- 6 Aph3 -Ic, ant2''-ia,aada1, 2xAadB, StrA, StrB TetA, TetG, ARG 3.49 OXA-69, VEB-1, 2xBLA, BlaZn, BlaMt -- CatA1, CmlA1, FloR Arr-2 4xSul-I, AYE TetR DfrA1, DfrA10 26 RF 2.28 Aph3 -Ic, AadA1, AadB, StrA, StrB OXA-69, VEB-1 -- CatA1, CmlA1, FloR Arr-2 Sul-I TetA, TetG, DfrA1, DfrA10 16 OXA-23, 2x66, TEM-1, 98, 2xBLA, BlaZn, ARG 3.5 Aac3 -Ia, Aac6 -Ib, 2xAadA1, Aph3 -Ic, StrA, StrB TCDC-AB0715 BlaMt xSul-I, Sul-II Tet-B RF 2.29 Aac3 -Ia, Aac6 -Ib, AadA1, Aph3 -Ic, StrA, StrB OXA-23, TEM Sul-I, Sul-II Tet-B AB ARG OXA-69, 2xBLA, BlaZn, BlaMt RF OXA ABSDF ARG OXA-66, 75, 2xBLA, BlaZn, BlaMt RF OXA AB D ARG -- - OXA-180,3xBLA, BlaZn, BlaMt RF OXA TYTH-1 ARG Aac6 -Ib, AadA1, ArmA, StrA, StrB OXA-66, 3xBLA,BlaZn, BlaMt MphE, MsrE catb8 -- Suli, Sul-II TetB RF Aac6 -Ib, AadA1, ArmA, StrA, StrB OXAA-66 MphE, MsrE -- Sul-I, Sul-II TetB AB210 ARG 3.41 Aac6 -Ib, AadA1, Aph3 -Ic, ArmA, StrA, StrB OXA-23, 66, 2xBLA,BlaZn, BlaMt MphE, MsrE CatB 8 -- Sul-I TetB RF 2.28 Aac6 -Ib, AadA1, Aph3 -Ic, ArmA, StrA, strb OXA-23 MphE, MsrE, -- Sul-I TetB AB900 ARG OXA-98, 2xBLA,BlaZn, BlaMt RF OXA AB4190 ARG 3.21 AadA1 OXA-64, 2x72, CTX-M-32, 2xBLA,BlaZn, BlaMt DfrA1 8 RF 2.34 AadA1 OXA-72, CTX-M-32, DfrA1 4 AB ARG 3.38 AadB, Aph3 -Ia OXA-69, 3xBLA, BlaZn, BlaMt Sul-II RF 2.28 AadB, Aph3 -Ia OXA Sul-II ABAC12 ARG 3.41 Aph3 -Ic, ArmA, StrA, StrB OXA-23, 66, TEM-1,3xBLA, BlaZn, BlaMt MsrE, MphE TetB RF 2.31 Aph3 -Ic, ArmA, StrA, StrB OXA-23, TEM-1 MsrE, MphE TetB -- 9 ABIS123 ARG 3.33 AadB, Aph6A OXA-71, 3xBLA, BlaZn, BlaMt Sul-I Tet-A, Tet-R RF 2.33 AadB, Aph6A OXA Sul-I Tet-A -- 5 ABNIH1 ARG 3.38 AadA23, Acc3 -Ia, OXA-82, 3xBLA,BlaZn, BlaMt Sul-I RF 2.41 Acc3`-Ia OXA Sul-I ABNIH2 ARG -- Aac3 -Ia, Aac6 -IL, StrA, StrB OXA-66, 72, 3xBLA,BlaZn, BlaMt Sul-II TetB RF -- Aac3 -Ia, Aac6 -IL, StrA, StrB OXA Sul-II TetB -- 7 ABNIH3 ARG -- Aac3 -Ia, Aac6 -IL, StrA, StrB OXA-72, 82, TEM-1, 2xBLA,BlaZn, BlaMt Sul-I TetB RF -- Aac3 -Ia, Aac6 -IL, StrA, StrB OXA-82, TEM TetB -- 7 ABNIH4 ARG OXA-82, 3xBLA, BlaZn, BlaMt RF OXA ABNIH5 ARG -- Aac3 -Ia, Aad23 OXA-82, 3xBLA,BlaZn, BlaMt Sul-I RF -- Aac3 -Ia OXA Sul-I ABNIH6 ARG -- Aac3 -Ia, Aph3 -Ia, 2xAph3 -VIb, 3x AadA1, Sat2A, StrB OXA-23, 69, 3xBLA,BlaZn, BlaMt Sul-I, Sul-II TetB DfrA1 20 RF -- Aac3 -Ia, Aph3 -Ia, Aph3 -VIb, AadA1, StrB OXA SUl-I, Sul-II TetB DfrA1 10 ABNIH7 ARG -- Aac3 -Ia, Aph3 -Ic, Aph3 -VIa, Aph3 -VIb, AadA1, Sat2A, OXA-23, 69, 3xBLA,BlaZn, BlaMt Sul-I, Sul-II TetB DfrA1 17 Tet Tmt No

6 StrB RF -- Aac3 -Ia, Aph3 -Ic, Aph3 -VIa, AadA1, StrB OXA-23, Sul-I, Sul-II TetB DfrA1 10 ABNIH10 ARG -- Aac3 -Ia, Aph3 -Ic, Aph3 -VIa, Aph3 -VIb, AadA1, StrB OXA-23, 69, 3xBLA,BlaZn, BlaMt Sul-I, Sul-II TetB DfrA1 17 RF -- Aac3 -Ia, Aph3 -Ic, Aph3 -VIa, AadA1, StrB OXA-23, Sul-I, Sul-II TetB DfrA1 10 ABNIH11 ARG -- Aac3 -Ia, Aph3 -Ia, Aph3 -Ib, Aph3 -VIa, 3xAadA1, Sat2A, StrB OXA-23,69, 3xBLA,BlaZn, BlaMt SulI,Sul-II TetB DhfrA1 20 RF -- Aph3 -Ic, Aph3 -VIa, StrB OXA Sul-I, Sul-II TetB DhfrA1 8 ABNIH13 ARG -- Aac3 -Ia, Aac6 -Iaf, AadA1, StrA, StrB OXA-66, 2x72, 2xBLA,BlaZn, BlaMt Sul-II TetB RF -- Aac3 -Ia, Aac6 -Iaf, StrA, StrB OXA Sul-II TetB -- 7 ABNIH14 ARG -- Aac3 -Ia, Aac6 -Iaf, AadA1, StrA, StrB OXA-66, 72, 2x207, 2xBLA,BlaZn, BlaMt Sul-II TetB RF -- Aac3 -Ia, Aac6 -Iaf, StrA, StrB OXA Sul-II TetB -- 7 ABNIH15 ARG -- Aac3 -Ia, Aac6 -Iaf, AadA1, StrA, StrB OXA-66, 72, 2x207, 2xBLA,BlaZn, BlaMt Sul-II TetB RF -- Aac3 -Ia, Aac6 -Iaf, StrA, StrB OXA Sul-II TetB -- 7 ABNIH16 ARG -- Aac3 -Ia, Aac6 -Iaf, AadA1, StrA, StrB OXA-66, 72, 2x207, 2xBLA,BlaZn, BlaMt Sul-II TetB RF -- Aac3 -Ia, Aac6 -Iaf, StrA, StrB OXA Sul-II TetB -- 7 ABNIH17 ARG -- Aac3 -Ia, Aac6 -Iaf, AadA1, StrA, StrB OXA-66, 72, 2xBLA,BlaZn, BlaMt Sul-II TetB RF -- Aac3 -Ia, Aac6 -Iaf, StrA, StrB OXA Sul-II TetB -- 7 ABNIH18 ARG OXA-82, 3xBLA,BlaZn, BlaMt RF OXA ABNIH19 ARG -- Aac3 -Ia, Aph3 -Ic, Aph3 -VIa, Aph3 -VIb, AadA1, Sat2A, StrB OXA-23, 69, 3xBLA,BlaZn, BlaMt SulI, Sul-II TetB DfrA1 18 RF -- Aac3 -Ia, Aph3 -Ic, Aph3 -VIa,, StrB OXA SulI, Sul-II TetB DfrA1 7 ABNIH20 ARG -- Aac3 -Ia, Aac6 -Iaf, AadA1, StrA, StrB OXA-66, 72, 2xBLA,BlaZn, BlaMt Sul-II TetB RF -- Aac3 -Ia, Aac6 -Iaf, StrA, StrB OXA Sul-II TetB -- 7 ABNIH22 ARG -- Aac3 -Ia, Aac6 -Iaf, AadA1, StrA, StrB OXA-66, 72, 2xBLA,BlaZn, BlaMt Sul-II TetB RF -- Aac3 -Ia, Aac6 -Iaf, StrA, StrB OXA Sul-II TetB -- 7 ABNIH23 ARG -- Aac3 -Ia, Aac6 -Iaf, AadA1, StrA, StrB OXA-66, 72, 2xBLA,BlaZn, BlaMt Sul-II TetB RF -- Aac3 -Ia, Aac6 -Iaf, StrA, StrB OXA Sul-II TetB -- 7 ABNIH24 ARG -- Aac3 -Ia, Aac6 -Iaf, AadA1, StrA, StrB OXA-66, 72, 3xBLA,BlaZn, BlaMt Sul-II TetB RF -- Aac3 -Ia, Aac6 -Iaf, StrA, StrB OXA Sul-II TetB -- 7 ABNIH25 ARG -- Aac3 -Ia, Aad23 OXA-82, 3xBLA,BlaZn, BlaMt Sul-I -- 9 RF -- Aac3 -Ia OXA Sul-I -- 3 ABNIH26 ARG -- Aac3 -Ia, Aac6 -Iaf, AadA1, StrA, StrB OXA-66, 72, 2xBLA,BlaZn, BlaMt Sul-II TetB RF -- Aac3 -Ia, Aac6 -Iaf, StrA, StrB OXA Sul-II TetB -- 7 AB515-8 ARG 3.39 AadA1, Aph3``-Ic, Aac6`-Ib, ArmA, StrA, StrB, OXA-66, TEM-1, 3xBLA,BlaZn, BlaMt -- CatB -- Sul-I, Sul-II TetB RF 2.48 AadA1, Aph3``-Ic, ArmA, StrA, StrB, OXA-66, TEM-1 -- CatB -- Sul-I, Sul-II TetB AB ARG OXA-88, 2xBLA, BlaZn, BlaMt RF OXA AB_15_45 ARG 3.33 Aac3`-Ia, Aac6`-IL, Aph3`-Ic, AadA15, AadA23, StrA, StrB, OXA-82, TEM-63, 2xBLA, BlaZn, BlaMt Sul-I, Sul-II TetB RF 2.36 Aac3`-Ia, Aac6`-IL, Aph3`-Ic, StrA OXA-82, TEM-63, Sul-I, Sul-II TetB -- 9 AB ARG 2.58 Aac3`-Ia, Aph3`-Ic, AadA1, StrA, StrB OXA-82, TEM-63, 2xBLA, BlaZn, BlaMt Sul-I TetB RF 2.3 Aac3`-Ia, Aph3`-Ic, AadA1, StrA, StrB OXA-66, TEM-1, Sul-I TetB -- 9 ABA118 ARG OXA-89, TEM-1, 2xBLA, BlaZn, BlaMt RF OXA-89, TEM AB-IS58 ARG -- Aac6 -Ib, Aph3 -VIa, AadA2, AadB, StrA, StrB OXA-69, 146, GES-11, 3xBLA, BlaZn, BlaMt Sul-I -- Dhfr7 16 RF -- Aac6 -Ib, Aph3 -VIa, AadA2, AadB, StrA, StrB OXA-23, GES Sul-I -- DfrA7 10 ABIS116 ARG 2.58 StrA, StrB OXA-91, 3xBLA,BlaZn Sul-II RF 2.29 StrA, StrB OXA Sul-II ABIS235 ARG 2.30 Aac6`-Ib, AadA2, AadB, Aph6A, StrA, StrB GES-11, OXA-23, 69, 3xBLA,BlaZn -- Cml -- Sul-I -- Dhfr7 16 RF 2.23 Aac6`-Ib, AadA2, AadB, Aph6A, StrA, StrB GES-11, OXA Cml -- Sul-I -- Dhfr7 11 AB-IS116 ARG -- StrA, StrB OXA-91, 3xBLA,BlaZn Sul-II RF -- StrA, StrB OXA Sul-II AB-IS143 ARG -- Aac3 -Ia, Aph3 -Ic, AadA1 OXA-117, TEM-1,3xBLA, BlaZn, BlaMt Sul-I RF -- Aac3 -Ia, Aph3 -Ic, AadA1 OXA-117, TEM Sul-I AB-IS251 ARG -- Aac6 -Ib, Aph3 -VIa, AadA3, StrA, StrB OXA-23, 69, GES-11, 3xBLA, BlaZn, BlaMt -- cmla5 -- Sul-I -- Dhfr7 16 RF -- Aac6 -Ib, Aph3 -VIa, AadA3, StrA, StrB OXA-23, GES cmla1 -- Sul-I -- dfra7 10 AB056 ARG 3.38 Aac3 -Ia,, AadA1 OXA-23, 69, 2xBLA, BlaMt Sul-I RF 2.33 Aac3 -Ia, AadA1 OXA Sul-I AB059 ARG -- Aac3 -Ia, AadA1, Aph3 -VIa, AadA3 OXA-69, 2xBLA,BlaZn, BlaMt RF -- Aac3 -Ia, AadA1, Aph3 -VIa OXA AB ARG -- Aac3 -Ia, Aac6 -IL, AadA1, StrA, StrB OXA-66, TEM-1,3xBLA, BlaZn, BlaMt Sul-II TetB -- 14

7 RF -- Aac3 -Ia, StrA, StrB OXA TetB -- 5 AB ARG -- AadB, AadA5, Aph3 -VIa OXA-2, 65, TEM-1, 2xBLA, BlaZn, BlaMt -- flor -- Sul-I TetG, TetR RF -- AadB, AadA5, Aph3 -VIa OXA-2, TEM-1 -- FloR -- Sul-I TetG -- 8 AB ARG -- AadB, Aph3 -VIa OXA-242, 23, TEM-1,2xBLA,BlaZn, BlaMt MsrE, MphE CAtB3 -- Sul-I RF -- AadB, Aph3 -VIa OXA-23 MsrE, MphE CAtB3 -- Sul-I AB ARG -- Aac6 -Ib, Aph3 -Ic, AadA1, StrA, StrB OXA-66, TEM-1, 3xBLA,BlaZn, BlaMt -- catb8 -- Sul-II TetB RF -- Aac6 -Ib, AadA1 Aph3 -Ic, StrA, StrB OXA-66, TEM-1 -- CAtB TetB -- 9 AB ARG -- OXA-64, BLA,BlaZn, BlaMt RF -- OXA AB ARG -- OXA-64, 2xBLA,BlaZn, BlaMt RF -- OXA AB ARG -- Aac6 -Ib, Aph3 -Ic, AadA1, ArmA, StrA, StrB OXA-66, 237, TEM-1, 2xBLA, BlaMt MsrE, MphE catb8 -- Sul-I, Sul-II TetB RF -- Aac6 -Ib, Aph3 -Ic, AadA1, ArmA, StrA, StrB OXA-66, TEM-1 MsrE, MphE catb3 -- Sul-I, Sul-II TetB AB1H8 ARG -- Aac3 -Ia, AadA1, Aph3 -Ic, StrA, StrB OXA-66,TEM-1,3xBLA, BlaZn, BlaMt MsrE, MphE -- Sul-I TetB, Tet RF -- Aac3 -Ia, AadA1, Aph3 -Ic, StrA, StrB OXA-66, TEM-1 MsrE, MphE -- Sul-I TetB,Tet AB3909 ARG -- AadB, Aph3 -Ic OXA-58, 90, 3xBLA, BlaZn, BlaMt -- flor -- Sul-III RF -- AadB, Aph3 -Ic OXA FloR -- Sul-II AB3990 ARG -- Aac6 -Ib, Aph3 VIa OXA-20, 58, 66,164, 3xBLA,BlaZn, BlaMt Sul-I RF -- Aac6 -Ib, Aph3 VIa OXA Sul-I AB4190 ARG -- AadA1, Sat2A OXA-64, 72, 72, TEM-1, 3xBLA, BlaZn, BlaMt DFrA 10 RF -- AadA1 OXA-72,CTX-M DfrA1 4 AB4857 ARG -- AadB, Aph3 VIa OXA-71 3xBLA,BlaZn, BlaMt Sul-I TetA, TetR RF -- AadB, Aph3 VIa OXA Sul-I TetA -- 5 AB4A3 ARG -- OXA-23, 99, 3xBLA, BlaZn, BlaMt Tet RF -- OXA Tet AB5075 ARG -- Aac3 -IIa, StrA, StrB OXA-64, 3xBLA, BlaZn, BlaMt Sul-II TetB RF -- Aac3 -IIa, StrA, StrB OXA Sul-II TetB -- 6 AB5256 ARG -- Aac3 -IIa, StrA, StrB OXA-64, 3xBLA, BlaZn, BlaMt Sul-II TetB RF -- Aac3 -IIa, StrA, StrB OXA Sul-II TetB -- 6 AB53264 ARG -- Aac3 -Ia, Aac6 -Iaf, Aph3 -Ic, Aph3 -VIa, AadA, StrA, StrB OXA-23,66, TEM-1, 3xBLA, BlaZn, BlaMt Sul-I, Sul-II TetB RF -- Aac3 -Ia, Aac6 -Iaf, Aph3 -Ic, Aph3 -VIa, StrA, StrB OXA-23, TEM Sul-I, Sul-II TetB AB ARG -- Aac3 -Ia, Aac6 -Iaf, Aph3 -Ic, Aph3 -VIa, AadA, StrA, StrB OXA-23,66, TEM-1, 3xBLA, BlaZn, BlaMt Sul-I, Sul-II TetB RF -- Aac3 -Ia, Aac6 -Iaf, Aph3 -Ic, Aph3 -VIa, StrA, StrB OXA-23, TEM Sul-I, Sul-II TetB AB ARG -- AadB, Aph3 -Ia OXA-69, 2xBLA, BlaMt Sul-III RF -- AadB, Aph3 -Ia OXA Sul-II AB ARG -- Aac3 -Ia, AadA1, Aph3-Ic, StrA, StrB OXA-66, TEM-1, 2xBLA,BlaZn, BlaMt Sul-I TetB RF -- Aac3 -Ia, AadA1, Aph3-Ic, StrA, StrB OXA-66, TEM Sul-I TetB -- 9 AB_ ARG OXA-91,3xBLA, BlaZn, BlaMt RF OXA AB_ ARG OXA-91, 3xBLA, BlaZn, BlaMt RF OXA AB_ ARG OXA-91, 3xBLA, BlaZn, BlaMt RF OXA AB ARG -- Aph3 -VIa OXA-23, 82, 3xBLA, BlaZn, BlaMt MsrE, MphE Sul-I RF -- Aph3 -VIa OXA-23 MsrE, MphE Sul-I AB ARG OXA-23, 146, 3xBLA, BlaZn, BlaMt SulI RF OXA Sul-I catb2, catb3, ARG -- AadA2, AadB OXA-64,CARB-2, 3xBLA, BlaZn, BlaMt MsrE, MphE AB cmla5 -- 2xSul-I -- dhfra19 17 RF -- AadA2, AadB OXA-64,CARB-2 MsrE, MphE catb2, catb3, cmla -- Sul-I -- Dfra18 11 AB ARG -- Aph3 -Ia, Aph6 -VIa, AadA2, AadB, StrA, StrB OXA-223, 3xBLA, BlaZn, BlaMt -- cmla5 -- Sul-I, Sul-II TetA dhfra 16 7 RF -- Aph3 -Ia, Aph6 -VIa, AadA2, AadB, StrA, StrB OXA cmla -- Sul-I, Sul-II TetA DfrA1 11 AB ARG -- Aac3 -Ia, Aac6 -Il, Aph3 -Ia AadA, StrA, StrB OXA-82, TEM-1, 3xBLA, BlaZn, BlaMt Sul-I, Sul-II TetB RF -- Aac3 -Ia, Aac6 -Il, Aph3 -Ic, StrA, StrB OXA-82, TEM Sul-I, Sul-II TetB AB ARG OXA-23, 146, 3xBLA, BlaZn, BlaMt Sul-I RF OXA AB ARG -- Aac6 -Il, Aph3 -Ic, AadA1, StrA, StrB, OXA-82, TEM-1, PER-1, 2xBLA, BlaZn, BlaMt Sul-I, Sul-II TetA RF -- Aac6 -Il, Aph3 -Ic, StrA, StrB OXA-82, TEM-1, PER Sul-I, Sul-II -- 9 AB ARG -- Aac6 -Ib, Aph3 -Ic, Aph3 -VIa, AadA, ArmA StrA, StrB, OXA-109, 235, TEM-1, 3xBLA, BlaZn MsrE, MphE catb TetB RF -- Aac6 -Ib, Aph3 -Ic, Aph3 -VIa, AadA, ArmA, StrA, StrB, OXA-109, TEM-1 MsrE, MphE catb TetB AB ARG -- Aph3 -la, Aph3 -VIa, AadA2, AadB OXA-237, 242, BLA, BlaZn, BlaMt -- Sul-I,

8 AB AB AB-TG19617 AB-AA014 AB-405E4 AB-ISacColiR AB-ISacColiS AB-6976 AB-TG2018 AB-TG2022 AB-TG2023 AB-TG2026 AB-TG2032 AB-TG2631 AB-TG5064 AB-TG27323 AB-TG27327 AB-TG27331 AB-TG27335 AB-TG27339 AB-TG27343 AB AB AB AB AB-W7282 AB-WC136 AB-WC141 AB-WC323 AB-WC348 RF -- Aph3 -la, Aph3 -VIa, AadA2, AadB OXA Sul-I -- 6 ARG OXA-69, 146, 3xBLA, BlaZn, BlaMt RF OXA ARG -- StrA, StrB OXA-23, 66, TEM-1, 3xBLA,BlaZn, BlaMt Sul-II TetB RF -- StrA, StrB OXA-23, TEM Sul-II TetB -- 6 ARG OXA-98, 3xBLA, BlaZn, BlaMt Sul-II RF OXA Sul-II ARG OXA-65,3xBLA,BlaZn, BlaMt RF OXA ARG OXA-217, 3xBLA, BlaZn, BlaM RF OXA ARG -- Aph3 -VIa, AadA2, AadB OXA-23, 82,3xBLA, BlaZn, BlaMt Sul-I RF -- Aph3 -VIa, AadA2, AadB OXA Sul-I ARG -- Aph3 -VIa, AadA2, AadB OXA-23, 82, 3xBLA, BlaZn, BlaMt Sul-I RF -- Aph3 -VIa, AadA2, AadB OXA Sul-I ARG -- Aac6 -Ib, Aph3 -VIa, AadA1, ArmA, StrA, StrB OXA-146, 2x206, 3xBLA, BlaZn, BlaMt MphE catb8 -- 2XSul-I TetB RF -- Aac6 -Ib, Aph3 -VIa, AadA1, ArmA, StrA, StrB OXA-23 MphE catb3 -- TetB ARG -- Aph3 -VIa, AadB OXA-237, 242, 3xBLA, BlaZn, BlaMt MsrE, MphE catb3 -- Sul-I RF -- Aph3 -VIa, AadB OXA-223 MsrE, MphE catb3 -- Sul-I -- 7 ARG -- StrA, StrB OXA-66, 3xBLA, BlaZn, BlaMt Sul-II TetB RF -- StrA, StrB OXA Sul-II Tet B -- 5 ARG -- StrA, StrB OXA-66, 3xBLA, BlaZn, BlaMt Sul-II TetB RF -- StrA, StrB OXA Sul-II -- 4 ARG -- Aac6 -Ib, AadA1, StrA, StrB OXA-66, 237, TEM-1, 3xBLA, BlaZn, BlaMt -- catb8 -- Sul-I TetB RF -- Aac6 -Ib, AadA1, StrA, StrB OXA-66, TEM Sul-I TetB -- 8 ARG -- Aph3 -Ic, AadA2, AadB OXA-223, 3xBLA, BlaZn, BlaMt Sul-I RF -- Aph3 -Ic, AadA2, AadB OXA Sul-I -- 5 ARG -- Aac6 -Ib, Aph3 -Ic, AadA1, ArmA, StrA, StrB OXA-66, 3xBLA, BlaZn, BlaMt MsrE, MphE catb8 -- Sul-I, Sul-II TetB RF -- Aac6 -Ib, Aph3 -Ic, AadA1, ArmA, StrA, StrB OXA-66 MsrE, MphE catb3 -- Sul-I, Sul-II TetB ARG -- StrA, StrB OXA-66, 3xBLA, BlaZn, BlaMt Sul-III TetB RF -- StrA, StrB OXA Sul-II TetB -- 5 ARG -- Aac6 -Ib, Aph3 -Ic, AadA1, ArmA, StrA, StrB OXA-66, TEM-1,2xBLA,BlaZn, BlaMt MsrE, MphE catb3 -- Sul-I, Sul-II TetB RF -- Aac6 -Ib, Aph3 -Ic, AadA1, ArmA, StrA, StrB Oxa-66 MsrE, MphE catb3 -- Sul-I, Sul-II TetB ARG -- Aac6 -Ib, Aph3 -Ic, AadA1, ArmA, StrA, StrB OXA-66, 233, TEM-1, 3xBLA, BlaZn, BlaMt MsrE, MphE catb8 -- Sul-I, Sul-II TetB RF -- Aac6 -Ib, Aph3 -Ic, AadA1, ArmA, StrA, StrB OXA-66, TEM-1 MsrE, MphE catb3 -- Sul-I, Sul-II TetB ARG -- Aac6 -Ib, Aph3 -Ic, AadA1, ArmA, StrA, StrB OXA-66, 233, 3xBLA, BlaZn, BlaMt MsrE, MphE catb8 -- Sul-I, Sul-II TetB RF -- Aac6 -Ib, Aph3 -Ic, AadA1, ArmA, StrA, StrB OXA-66 MsrE, MphE catb3 -- Sul-I, Sul-II TetB ARG -- Aac6 -Ib, Aph3 -Ic, AadA1, ArmA, StrA, StrB OXA-66, 233, 3xBLA, BlaZn, BlaMt MsrE, MphE catb8 -- Sul-I, Sul-II TetB RF -- Aac6 -Ib, Aph3 -Ic, AadA1, ArmA, StrA, StrB OXA-66 MsrE, MphE catb3 -- Sul-I, Sul-II TetB ARG OXA-91, 3xBLA,BlaZn RF OXA ARG -- Aph3 -VIa, AadA5, AadB OXA-2, 65, TEM-1, 2xBLA, BlaZn, BlaMt -- flor -- Sul-I, Sul-II TetG, TetR RF -- Aph3 -VIa, AadA5, AadB OXA-2, TEM-1 -- flor -- Sul-I, Sul-II TetG -- 9 ARG -- StrA, StrB OXA-71, TEM-1, 3xBLA, BlaZn, BlaMt TetB RF -- StrA, StrB OXA-71, TEM TetB -- 5 ARG -- StrA, StrB OXA-71, TEM-1,3xBLA,BlaZn, BlaMt TetB RF -- StrA, StrB OXA-71, TEM TetB -- 5 ARG OXA-51, 3xBLA, BlaZn, BlaMt RF OXA ARG OXA-82, 3xBLA,BlaZn, BlaMt RF OXA ARG -- Aac6 -Ib, Aph3 -Ia, AadA1, ArmA, StrA, StrB OXA-23, 66, 3xBLA, BlaZn, BlaMt MsrE, MphE CatB8 -- Sul-I TetB RF -- Aac6 -Ib, Aph3 -Ic, ArmA, StrA, StrB OXA-23 MsrE, MphE catb3 -- Sul-I TetB ARG OXA-116, 2xBLA, BlaZn RF OXA ARG OXA-111, 2xBLA, BlaZn, BlaMt RF OXA ARG -- Aac6 -Ir RF -- Aac6 -Ir ARG -- StrA, StrB OXA-208, 3xBLA, BlaMt Sul-II RF -- StrA, StrB OXA Sul-II

9 AB-WC487 AB-WC692 AB-WC-A-92 AB-WC-A-694 AB-ZWS1122 AB-ZWS1219 AB-OIFC021 AB-OIFC032 AB-OIFC035 AB-OIFC047 AB-OIFC065 AB-OIFC074 AB-OIFC087 AB-OIFC098 AB-OIFC099 AB-OIFC0109 AB-OIFC0110 AB-OIFC0111 AB-OIFC0137 AB-OIFC0143 AB-OIFC0162 AB-OIFC0180 AB-OIFC0189 AB-OIFC0338 AB-TG19582 AB-UMB001 AB-UMB002 AB-AC30 AB-ATCC19606 AB-BC1 ARG xBLA, BlaZn, BlaMt RF ARG -- Aph3 -VIb, 2xStrA, StrB OXA-90, PER-1, 3xBLA, BlaZn, BlaMt RF -- Aph3 -VIb, StrA, StrB OXA-90, PER ARG OXA-117, 3xBLA, BlaZn, BlaMt RF OXA ARG -- Aph3 -VIb, AadB OXA-71, 3xBLA, BlaZn, BlaMt Sul-I TetA, TetR RF -- Aph3 -VIb, AadB OXA Sul-I TetA -- 5 ARG -- Aac6 -Ib, AadA1, AadA15, ArmA, StrA, StrB OXA-23, 66, TEM-1, 3xBLA, BlaZn, BlaMt MsrE, MphE catb8 -- 2xSul-I TetB RF -- Aac6 -Ib, AadA1, AadA15, ArmA, StrA, StrB OXA-23, TEM-1 MsrE, MphE catb3 -- Sul-I TetB ARG -- Aac6 -Ib, 2xAadA1, ArmA, StrA, StrB OXA-23, 66, TEM-1, 3xBLA, BlaZn, BlaMt MsrE, MphE -- 2xSul-I TetB RF -- AadA1, ArmA, StrA, StrB OXA-23, TEM-1 MsrE, MphE catb3 -- Sul-I TetB ARG xBLA, BlaZn, BlaMt RF ARG OXA-100,, 3xBLA, BlaZn, BlaMt RF OXA ARG OXA-106, 3xBLA, BlaZn, BlaMt RF OXA ARG OXA-208, 3xBLA, BlaZn, BlaMt RF OXA ARG -- AadB, StrA, StrB OXA-23, 3xBLA, BlaZn, BlaMt Sul-II RF -- AadB, StrA, StrB OXA Sul-II ARG -- Aph3 -Ic OXA-23, 3xBLA, BlaZn, BlaMt -- cata1, 2xcmlA5 -- Sul-II TetB RF -- Aph3 -Ic OXA cata1, cmla1 -- Sul-II TetB -- 6 ARG OXA-100, 3xBLA, BlaZn, BlaMt RF OXA ARG -- StrA, StrB OXA-68, CMY-30, 3xBLA, BlaZn, BlaMt -- flor -- Sul-II TetA, TetR RF -- StrA, StrB OXA flor -- Sul-II TetA -- 6 ARG -- StrA, StrB OXA-100, 3xBLA,BlaZn, BlaMt Sul-II TetB RF -- StrA, StrB OXA Sul-II TetB -- 5 ARG -- AadB,, StrA, StrB OXA-71, 3xBLA, BlaZn, BlaMt Sul-I, Sul-II TetA, TetR RF -- AadB,, StrA, StrB OXA Sul-I, Sul-II TetA -- 7 ARG OXA-90, 3xBLA, BlaZn, BlaMt RF OXA ARG OXA-98, 3xBLA, BlaZn, BlaMt RF OXA ARG -- Aph3 -VIb, AadB, StrA, StrB OXA-71, 3xBLA, BlaZn, BlaMt Sul-I, Sul-II TetA, TetR RF -- Aph3 -VIa, AadB, StrA, StrB OXA Sul-I, Sul-II TetA -- 8 ARG -- Aph3 -Ic, AadA2, AadA13, AadB, 2xStrA, 2xStrB OXA-64, PER-1, 3xBLA, BlaMt xSul-I, Sul-II RF -- Aph3 -Ic, AadA2, AadA13, AadB, StrA, StrB OXA-64, PER Sul-I, Sul-II ARG OXA-208, 3xBLA,BlaZn, BlaMt RF OXA ARG -- Aac3 -Ia, Aph3 -Ia, 2x Aph3 -VIa AadA1, AadB, StrA, StrB OXA-66, TEM-1,2xBLA,BlaZn, BlaMt Sul-I, Sul-II TetB RF -- Aac3 -Ia, Aph3 -Ic, AadA1, AadB, StrA, StrB OXA-66, TEM Sul-I, Sul-II TetB ARG -- Aac3 -Ia, Aac6 -Ib, Aph3 -Ic, AadA1, StrA, StrB OXA-66, TEM-1, 3xBLA, BlaZn, BlaMt Sul-I TetB RF -- Aac3 -Ia, Aac6 -Ib, Aph3 -Ic, AadA1, StrA, StrB OXA-66, TEM Sul-I TetB ARG -- Aac3 -IIa, Aph3 -Ia, Aph3 -VIa, StrA, StrB OXA-66, TEM-1,3xBLA, BlaZn, BlaMt -- cata1 -- Sul-I TetB RF -- Aac3 -IIa, Aph3 -Ic, Aph3 -VIa, StrA, StrB OXA-66, TEM-1 -- cata1 -- Sul-II TetB ARG OXA-69, 3xBLA, BlaZn, BlaMt Sul-II RF OXA Sul-II ARG -- Aac3 -Ia, Aph3 -Via, AadA1 OXA-23, 82, 3xBLA, BlaZn, BlaMt MsrE, MphE xSul-I RF -- Aph3 -Via, AadA1, AadA22 OXA-23 MsrE, MphE Sul-I ARG OXA-51, 3xBLA, BlaZn, BlaMt Sul-II RF OXA ARG -- Aph3 -Ic, ArmA, StrA, StrB OXA-23, 66, TEM-1,3xBLA, BlaZn, BlaMt MsrE, MphE TetB RF -- Aph3 -Ic, ArmA, StrA, StrB OXA-23, TEM-1 MsrE, MphE TetB -- 9 ARG OXA-53, 3xBLA, BlaZn, BlaMt Sul-II -- 7 RF OXA Sul-II -- 2 ARG -- Aac3 -Ia, AadA1 OXA-23, 69, 3xBLA, BlaZn, BlaMt cata1 -- Sul-I TetA, TetR RF -- Aac3 -Ia, AadA1 OXA-23 cata1 -- Sul-I TetA -- 6

10 AB-BC5 ARG -- Aac3 -Ia, AadA1 OXA-23,23, 23, 693xBLA, BlaZn, BlaMt cata1 -- 2xSul-I TetA, TetR RF -- Aac3 -Ia, AadA1 OXA-23 cata1 -- Sul-I TetA -- 6 ABBZICU-2 ARG -- Aac3 -IId OXA-51, 58, 3xBLA, BlaZn, BlaMt MsrE, MphE RF -- Aac3 -IId OXA-51 MsrE, MphE AB-MSP4-16 ARG OXA-51,3xBLA, BlaZn, BlaMt Sul-III -- 7 RF OXA Sul-II -- 2 AB-NAVAL13 ARG -- Aph3 -VIa, AadB, StrA, StrB OXA-71, 3xBLA, BlaZn, BlaMt Sul-I, Sul-II TetA, TetR RF -- Aph3 -VIa, AadB, StrA, StrB OXA Sul-I, Sul-II TetA -- 8 AB-NAVAL17 ARG -- Aac3 -Ia, Aph3 -Ic, AadA1, StrA, StrB OXA-23, TEM-1, 3xBLA, BlaZn, BlaMt Sul-I TetB RF -- Aac3 -Ia, Aph3 -Ic, AadA1, StrA, StrB OXA-66, TEM Sul-I TetB -- 9 AB-NAVAL57 ARG -- Aac3 -IId, Aph3 -VIb, StrA, StrB OXA-51, PER-1, 3xBLA, BlaZn, BlaMt -- flor -- Sul-II RF -- Aac3 -IId, Aph3 -VIb, StrA, StrB OXA-51, PER-1 -- flor -- Sul-II AB-NAVAL72 ARG OXA-71, 3xBLA, BlaZn, BlaMt RF OXA AB-NAVAL78 ARG -- Aac3 -Ia, AadA1 OXA-82, 3xBLA, BlaZn, BlaMt Sul-I RF -- Aac3 -Ia, AadA1 OXA-82, Sul-I AB-NAVAL81 ARG -- Aph3 -VIa, AadB OXA-71, 3xBLA, BlaZn, BlaMt Sul-I TetA, TetR RF -- Aph3 -VIa, AadB OXA Sul-I TetA -- 5 AB-NAVAL82 ARG OXA-65, 3xBLA, BlaZn, BlaMt RF OXA AB-NAVAL83 ARG -- Aph3 -VIa OXA-23, 3xBLA, BlaZn, BlaMt Sul-I RF -- Aph3 -VIa OXA Sul-I AB-TG2028 ARG -- Aph3 -Ic, AadA2, AadB OXA-223, 3xBLA, BlaZn, BlaMt Sul-I RF -- Aph3 -Ic, AadA2, AadB OXA Sul-I AB-TG2030 ARG -- Aph3 -Ia, AadA2, AadB OXA-223, 3xBLA, BlaZn, BlaMt Sul-I RF -- Aph3 -Ia, AadA2, AadB OXA Sul-I AB-TG2031 ARG -- Aph3 -Ia, AadA2, AadB OXA-233, 3xBLA,BlaZn, BlaMt Sul-I RF -- Aph3 -Ia, AadA2, AadB OXA Sul-I ABNIPH24 ARG -- Aac3 -Ia, Aph3 -Ia, AadA1, StrA, StrB OXA-66, TEM-1, 3xBLA, BlaZn, BlaMt -- CatA1 -- Sul-I, Sul-II TetB RF -- Aac3 -Ia, Aph3 -Ic, AadA1, StrA, StrB OXA-66, TEM-1 -- CatA1 -- Sul-I, TetB ABNIPH60 ARG OXA-69, 2xBLA, BlaZn, BlaMt RF OXA ABNIPH67 ARG OXA-51, 3xBLA, BlaZn, BlaMt RF OXA ABNIPH70 ARG OXA-180, 3xBLA, BlaZn, BlaMt RF OXA ABNIPH80 ARG OXA-78, 3xBLA, BlaZn, BlaMt RF OXA ABNIPH146 ARG OXA-64, 3xBLA, BlaZn, BlaMt RF OXA ABNIPH190 ARG OXA-64, 3xBLA, BlaZn, BlaMt RF OXA ABNIPH201 ARG OXA-208, 3xBLA, BlaZn, BlaMt Tet RF OXA Tet ABNIPH290 ARG -- Aac3 -Ia, Aph3 -Ia, 5xAph3 -VIa, AadA1 OXA-69, TEM-1 2xBLA, BlaZn, BlaMt -- CatA1 -- 2xSul TetA, TetR RF -- Aac3 -Ia, Aph3 -Ic, Aph3 -VIa, AadA1 OXA-69, TEM-1 -- CatA1 -- SulI TetA -- 9 ABNIPH329 ARG OXA-106, 3xBLA, BlaZn, BlaMt RF OXA ABNIPH235 ARG -- AadB, StrA, StrB OXA-128, 3xBLA, BlaZn, BlaMt Sul-I RF -- AadB, StrA, StrB OXA Sul-I ABNIPH527 ARG -- Aph3 -Ic, AadA1, StrA, StrB OXA-69, TEM-1, 3xBLA, BlaZn, BlaMt -- CatA1 -- Sul-I, Sul-II TetA, TetR DfrA5 17 RF -- Aph3 -Ic, AadA1, StrA, StrB OXA-69, TEM-1, 3xBLA, BlaZn, BlaMt -- CatA1 -- Sul-I, TetA DfrA5 11 ABNIPH528 ARG -- Aac3 -Ia, Aph3 -Ic, AadA1, StrA, StrB OXA-66, TEM-1, 3xBLA, BlaZn, BlaMt -- CatA1 -- Sul-I TetB RF -- Aac3 -Ia, Aph3 -Ic, AadA1, StrA, StrB OXA-66, TEM-1 -- CatA1 -- Sul-I TetB ABNIPH601 ARG OXA-69, 3xBLA, BlaZn, BlaMt RF OXA ABNIPH615 ARG OXA-51, 2xBLA, BlaZn, BlaMt RF OXA ABNIPH1362 ARG OXA-66, 3xBLA, BlaZn, BlaMt RF OXA ABNIPH1669 ARG -- Aph3 -VIa, AadB OXA-71, 3xBLA, BlaZn, BlaMt Sul-I

11 ABNIPH1734 ABNIPH2061 ABPR07 ABANC4097 RF -- Aph3 -VIa, AadB OXA Sul-I ARG -- Aph3 -VIa, AadB OXA-51, 58, 3xBLA, BlaZn, BlaMt RF -- Aph3 -VIa, AadB OXA ARG -- Aph3 -VIa, StrA, StrB OXA-109, 3xBLA, BlaZn, BlaMt Sul-II TetB RF -- Aph3 -VIa, StrA, StrB OXA Sul-II TetB -- 6 ARG OXA-51, 3xBLA, BlaZn, BlaMt RF OXA ARG -- Aac3 -Ia, Aph3 -VIa, AadA1, NDM-1, OXA-23,69, TEM-1, 3xBLA, BlaZn, BlaMt Sul-I TetA, TetR RF -- Aac3 -Ia, Aph3 -Ic, AadA1 OXA-69, TEM-1 -- CatA -- Sul-I TetA Reference List 1. Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O, Aarestrup FM, Larsen MV Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother 67:

12 Supplementary Table S4: List of additional genes detected using ARG-ANNOT in first 25 analyzed genomes. Strains Gene Probable reason AB1656 strb duplicate gene AB0057 aad1ac duplicate gene oxa-69/196 same group gene suli duplicate gene ABACICU oxa-18/ 53 same group gene ABMDRZJ06 oxa-66 same group gene aada1b closely related gene AYE ant2''-ia closely related gene 3xsul-I duplicate genes aada1b duplicate gene TCDC-AB0715 Tem98 closely related gene 2xoxa-66 duplicate gene sul-i duplicate gene ABSDF oxa-66 same group gene AB210 oxa-66 same group gene AB4190 oxa-72 duplicate gene oxa-64 same group gene ABAC12 oxa-66 closely related gene ABNIH1 aad23 closely related gene AB515-8 aac3`-ib closely related gene Ab_15_45 Aac61b* partial gene AB056 oxa-69 same group gene ABIS235 oxa-69 same group gene Common gene BLA no gene in the database BlaMt no gene in the database BlaZn no gene in the database

13 Supplementary Table S5: Comparative genome analysis for AR genes in diffrent strains of Staphylococcus aureus using ARG-ANNOT and Resfinder (1) (Agly-Aminoglycosides, Bla-Beta-lactamase,Flq- Flouroquinolones, Fos-Fosfomycin, MLS-Macrolide Lincosamide StreptograminB, Phe-Phenicol, Tet-Tetracycline, Tmt-Trimethoprime, ARG-ARGANNOT, RF-resfinder). Strains name AGly Bla Flq Fos MLS Phe Tet Tmt No ARG aac3 -IK*, aadd, aph, 2xspc meca nora fosb 2xermA dha1 tet-38 dfrc 13 RF aadd, spc meca nora -- erma -- tet BA02176 ARG aac3 -IK*, aph blaz, meca nora -- vgaa dha1* tet-m, tet-38 dfrc 10 RF -- blaz, meca nora -- vgaa -- tetm, tet Bmb9393 ARG aac3 -IK*, Aac6 -aph2, Aph3 -III, aph, ant6 -Ia, 2xspc, sta4a blaz, meca nora fosb 2xermA dha1* tet-m, tet-38 dfrc 18 RF aac6 -aph2, aph3 -III, ant6 -Ia, spc blaz, meca nora -- erma -- tetm, tet CA-347 ARG aac3 -IK*, aadd, aph, 2xspc meca nora -- 2xermA dha1* tet-38 dfrc 12 RF aadd, spc meca nora -- erma -- tet M1 ARG aac3 -IK*, aph, spc meca nora fosb erma dha1 tet-38* dfrc 10 RF spc meca nora -- erma -- tet RF122 ARG aac3 -IK*, aph -- nora dha1* tet-38* RF nora tet ARG aac3 -IK*, Aph3 -III, aph, ant6 -Ia, sta4a, blaz, meca nora -- mphc, msra dha1* tet RF aph3 -III, ant6 -Ia blaz, meca nora -- mphc, msra -- tet ARG aac3 -IK*, aph* blaz nora -- ermt dha1* tet RF -- blaz nora -- ermt -- tet Col ARG aac3 -IK, aph* meca nora fosb -- dha1* tet RF -- meca nora tet ECT-R2 ARG aac3 -IK*, aadd, aph, spc -- nora fosb erma -- tet RF aadd, spc -- nora -- erma -- tet ED98 ARG aac3 -IK*, aph* -- nora fosb -- dha1* tet RF nora tet ED133 ARG aac3 -IK*, aph* -- nora fosb -- dha1* tet RF nora tet JH1 ARG aac3 -IK*, aph*, 2xspc meca* nora fosb 2xermA dha1* tet-38 dfrc* 12 RF spc meca nora -- erma -- tet-38* MO-013 ARG aac3 -IK*, aph meca nora dha1 tet RF -- meca nora tet MSHT1132 ARG aph meca nora fosb -- dha1* tet RF -- meca nora tet MW2 ARG aac3 -IK*, aph* meca nora dha1 tet RF -- meca nora tet N315 ARG aac3 -IK*, aadd, aph, 5xspc meca nora fosb 5xermA dha1* tet-38 dfrc* 19 RF aadd, spc meca nora -- erma -- tet NCTC8325 ARG aac3 -IK*, aph -- nora fosb -- dha1* tet RF nora tet ST228 ARG aac3 -IK*, aph, 4xspc meca nora fosb 4xermA dha1* tet RF spc meca nora -- erma -- tet TW20 ARG aac3 -IK, Aac6 -aph2, Aph3 -III, aph*, ant6 -Ia, 2xspc, sta4a blaz, meca nora fosb 2xermA dha1* tet-m, tet-k, tet-38 dfrg 19 RF aac6 -aph2, aph3 -III, ant6 -Ia, spc blaz, meca nora -- erma -- tet-m, tet-k, tet-38 dfrg 12 *the gene is annotated by some other name in respective genome

14 Reference List 1. Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O, Aarestrup FM, Larsen MV Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother 67:

15 S6: Supplementary mutational gene database (1-11) >RpoB_C1564(64)A:A1565(65)T:A1565(65)G:G1573(73)T:A1574(74)T:C1603(103)G:C16 03(103)T:A1604(104)T:C1619(119)A:C1619(119)T:C1696(196)T:A1741(241)T TTGATTAATGCAAAACCAGTTGCTGCTGCAATCAAAGAATTCTTTGGTTCAAGCC AGTTATCTCAGTTTATGGACCAAAACAACCCACTATCTGAGATTACACATAAACG TCGTGTATCTGCGCTTGGTCCTGGTGGTTTAACACGTGAACGTGCGGGCTTCGAA GTACGTGACGTACACCAAACTCACTATGGTCGTGTTTGTCCAATTGAAACTCCTG AAGGTCCAAACATTGGTTTGATCAACTCGCTTTCTGTATACGCAAAAGCGAATGA CTTCGGTTTCTTGGAAACTCCATAC >GyrA1_T175(175)A:G224(224)C:C198(198)T:G259(259)T:G259(259)A ATGAGCGACCTTGCGAGAGAAATTACACCGGTCAACATTGAGGAAGAGCTGAAG AGCTCCTATCTGGATTATGCGATGTCGGTCATTGTTGGCCGTGCGCTGCCAGATGT CCGAGATGGCCTGAAGCCGGTACACCGTCGCGTACTTTACGCCATGAACGTACTA GGCAATGACTGGAACAAAGCCTATAAAAAATCTGCCCGTGTCGTTGGTGACGTAA TCGGTAAATACCATCCCCATGGTGACTCGGCGGTCTATGACACGATCGTCCGCAT GGCGCAGCCATTCTCGCTGCGTTAT >GyrA2_G2599(299)T GACGAAAGGGGTTATCTCCATCAAGGTTACCGAACGTAACGGTTTAGTTGTTGGC GCGGTACAGGTAGATGACTGCGACCAGATCATGATGATCACCGATGCCGGTACG CTGGTACGTACTCGCGTTTCGGAAATCAGCATCGTGGGCCGTAACACCCAGGGCG TGATCCTCATCCGTACTGCGGAAGATGAAAACGTAGTGGGTCTGCAACGTGTTGC TGAACCGGTTGACGAGGAAGATCTGGATACCATCGACGGCAGTGCCGCGGAAGG GGACGATGAAATCGCTCCGGAAGTGGA >ParC_G233(33)A:A238(38)C:G239(39)T:C240(40)A:G250(50)A: 682(482)G:G757(557)A CGGTGACGTACTGGGTAAATACCATCCGCACGGCGATAGCGCCTGTTATGAAGCG ATGGTCCTGATGGCGCAACCGTTCTCTTACCGTTATCCGCTGGTTGATGGTCAGGG GAACTGGGGCGCGCCGGACGATCCGAAATCGTTCGCGGCAATGCGTTACACCGA ATCCCGGTTGTCGAAATATTCCGAGCTGCTATTGAGCGAGCTGGGGCAGGGGACG GCTGACTGGGTGCCAAACTTCGACGGCACTTTGCAGGAGCCGAAAATGCTACCTG CCCGTCTGCCAAACATTTTGCTTAACGGCACCACCGGTATTGCCGTCGGCATGGC GACCGATATTCCACCGCATAACCTGCGTGAAGTGGCTCAGGCGGCAATCGCATTA ATCGACCAGCCGAAAACCACGCTCGATCAGCTGCTGGATATCGTGCAGGGGCCG GATTATCCGACTGAAGCGGAAATTATCACTTCGCGCGCCGAGATCCGTAAAATCT ACGAGAACGGACGTGGTTCAGTGCGTATGCGCGCGGTGTGGAAGAAAGAAGATG GCGCGGTGGTTATCAGCGCATTGCCGCATCAGGTTTCAGGTGCGCGCGTACT >16SrRNA_T1406(206)A:A1408(208)G:C1409(209)T:G1491(291)T ATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGA GAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATT GGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAA TGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGA GTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTG TGATTCATGACTGGGGTGAAGTCGTAA

16 >23SrRNA1_A2058(8)G:A2059(9)G:C2611(561)T AAGACGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAGCCT TGATGTGTAGGATAGGTGGGAGGCTTTGAAGTGTGGACGCCAGTCTGCATGGAGC CGACCTTGAAATACCACCCTTTAATGTTTGATGTTCTAACGTTGACCCGTAATCCG GGTTGCGGACAGTGTCTGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAG TAACGGAGGAGCACGAAGGTTGGCTAATCCTGGTCGGACATCAGGAGGTTAGTG CAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAG CAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAA AGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGG TGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCCCAAG GGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGA GACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCT >KatG_G871(171)A:G900(200)C:G944(244)C:G985(285)GG GGCCGAACGGCAACCCGGACCCCATGGCCGCGGCGGTCGACATTCGCGAGACGT TTCGGCGCATGGCCATGAACGACGTCGAAACAGCGGCGCTGATCGTCGGCGGTC ACACTTTCGGTAAGACCCATGGCGCCGGCCCGGCCGATCTGGTCGGCCCCGAACC CGAGGCTGCTCCGCTGGAGCAGATGGGCTTGGGCTGGAAGAGCTCGTATGGCAC CGGAACCGGTAAGGACGCGATCACCAGCGGCATCGAGGTCGTATGGACGAACAC CCCGACGAAATGGGACAACAGTTTCCTCG >pnca_a139(39)g:t192(92)ta ATGCGGGCGTTGATCATCGTCGACGTGCAGAACGACTTCTGCGAGGGTGGCTCGC TGGCGGTAACCGGTGGCGCCGCGCTGGCCCGCGCCATCAGCGACTACCTGGCCGA AGCGGCGGACTACCATCACGTCGTGGCAACCAAGGACTTCCACATCGACCCGGGT GACCACTTCTCCGGCACACCGGACTATTCCTCGTCGTGGCCACCGCATTGCGTCA GCGGTACTCCCGGCGCGGACTTCCATCCCAGTCTGGACACGTCGGCAATCGAGGC GGTGTTCTACAAGGGTGCCTACACC >EmbB_A916(16)G:A916(16)C:G918(18)T:G918(18)A:G918(18)C GGCTACATCCTGGGCATGGCCCGAGTCGCCGACCACGCCGGCTACATGTCCAACT ATTTCCGCTGGTTCGGCAGCCCGGAGGATCCCTTCGGCTGGTATTACAACCTGCT GGCGCTGATGACCCATGTCAGCGACGCCAGTCTGTGGATGCGCCTGCCAGACCTG GCCGCCGGGCTAGTGTGCTGGCTGCTGCTGTCGCGTGAGGTGCTGCCCCGCCTCG GGCCGGCGGTGGAGGCCAGCAAACCCGCCTACTGGGCGGCGGCCATGGTCTTGC TGACCGCGTGGATGCCGTTCAACAAC >rpsl_a128(128)g:a263(263)g ATGGCAACAGTTAACCAGCTGGTACGCAAACCACGTGCTCGCAAAGTTGCGAAA AGCAACGTGCCTGCGCTGGAAGCATGCCCGCAAAAACGTGGCGTATGTACTCGTG TATATACTACCACTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGT TCGTCTGACTAACGGTTTCGAAGTGACTTCCTACATCGGTGGTGAAGGTCACAAC CTGCAGGAGCACTCCGTGATCCTGATCCGTGGCGGTCGTGTTAAAGACCTCCCGG GTGTTCGTTACCACACCGTACGTGGT >pmra_g305(155)a GAGTTGCTTGCCCGTATTCATGCCTTACTACGCCGTAGTGGAGTAGAAGCTCAAC TTGCGAGTCAAGATCAACTATTAGAAAGTGGTGATCTGGTTTTAAATGTTGAACA GCATATTGCGACGTTTAAAGGCCAACGCATTGATTTATCAAATCGTGAATGGGCA ATCTTTYKAATTCCACTTATGACTCACCCAAATAAAATCTTTTCTAAAGCCAACTT

17 AGAAGATAAGTTATATGATTTTGATAGTGATGTGACCAGTAATACTATTGAAGTA TATGTTCACCATTTAAGAGCGAAG >pmrb1_c38(38)a:c41(41)t:g261(261)c:g261(261)t GTGCATTATTCATTAAAAAAACGACTGATTTGGGGCACCTCAATTTTCAGTGTCAT CTTAGGTTGTATTTTAATTTTTAGTGCTTATAAGGTTGCACTTCAAGAAGTCGATG AAATTCTAGATACTCAAATGAAGTATTTAGCGGAAAGAACAGCTGAGCACCCTTT AAAAACTGTAAGCAGTAAGTTTGATTTTCATAAAACTTACCACGAAGAAGATCTG TTTATCGATATCTGGGCTTATAAGGATCAGGCCCATTTGTCTCATCATTTACATTT GCTGGTTCCACCTGTTGAGCAA >pmrb2_t434(14)a:c680(260)t:c697(277)t:c697(277)a:g784(364)c CTTGCGGGCAGTATGTTTATTCCGTATTTAATTATTTTACCTTTTGCAATATTTGCC TTAGCAGCCATTATTCGTCGTGGTTTAAAACCAATAGATGATTTTAAAAATGAGT TAAAAGAACGCGATTCCGAAGAACTCACACCAATTGAAGTACATGATTATCCTCA AGAGCTTTTACCTACTATTGACGAAATGAACCGTCTTTTTGAGCGCATTTCTAAAG CGCAAAATGAACAGAAGCAATTTATTGCCGATGCTGCTCATGAATTACGAACACC TGTGACTGCATTGAACTTACAAACCAAGATTTTGCTAAGCCAGTTTCCTGAGCAT GAATCATTGCAAAACTTAAGCAAGGGTTTGGCGCGTATTCAGCATTT >pmrb3_t1057(7)a:t1160(110)a:c1208(158)t GTTATTAATATTTCAGTTTATACCGATCCAGATCACTACGCATGTATTCAAATTGA AGATAGCGGTGCAGGAATAGACCCTGAAAATTACGATAAAGTCCTTAAGCGTTTT TATCGCGTGCATCACCATCTTGAGGTGGGAAGTGGTCTAGGTTTATCTATTGTAG ATCGTGCAACTCAAAGGCTTGGTGGGACTTTAACTCTCGATAAGAGCTTAGAGCT TGGCGGTCTTTCTGTATTAGTGAAATTAC Reference List (1) Doktor SZ, Shortridge VD, Beyer JM, Flamm RK Epidemiology of macrolide and/or lincosamide resistant Streptococcus pneumoniae clinical isolates with ribosomal mutations. Diagn. Microbiol. Infect. Dis. 49(1): (2) Feuerriegel S, Oberhauser B, George AG, Dafae F, Richter E, Rusch-Gerdes S, Niemann S Sequence analysis for detection of first-line drug resistance in Mycobacterium tuberculosis strains from a high-incidence setting. BMC Microbiol. 12:90. (3) Froelich JM, Tran K, Wall D A pmra constitutive mutant sensitizes Escherichia coli to deoxycholic acid. J. Bacteriol. 188(3): (4) Fujimoto-Nakamura M, Ito H, Oyamada Y, Nishino T, Yamagishi J Accumulation of mutations in both gyrb and pare genes is associated with high-level resistance to novobiocin in Staphylococcus aureus. Antimicrob Agents Chemother. 49(9):

18 (5) Nessar R, Reyrat JM, Murray A, Gicquel B Genetic analysis of new 16S rrna mutations conferring aminoglycoside resistance in Mycobacterium abscessus. J. Antimicrob. Chemother. 66(8): (6) Shi D, Li L, Zhao Y, Jia Q, Li H, Coulter C, Jin Q, Zhu G Characteristics of embb mutations in multidrug-resistant Mycobacterium tuberculosis isolates in Henan, China. J. Antimicrob. Chemother. 66(10): (7) Ruiz J, Moreno A, Jimenez de Anta MT, Vila J A double mutation in the gyra gene is necessary to produce high levels of resistance to moxifloxacin in Campylobacter spp. clinical isolates. Int. J. Antimicrob. Agents. 25(6): (8) Bilgin N, Richter AA, Ehrenberg M, Dahlberg AE, Kurland CG Ribosomal RNA and protein mutants resistant to spectinomycin. EMBO J.9(3): (9) Herrera CM, Hankins JV, Trent MS Activation of PmrA inhibits LpxTdependent phosphorylation of lipid A promoting resistance to antimicrobial peptides. Mol. Microbiol. 76(6): (10) Moskowitz SM, Ernst RK, Miller SI PmrAB, a two-component regulatory system of Pseudomonas aeruginosa that modulates resistance to cationic antimicrobial peptides and addition of aminoarabinose to lipid A. J. Bacteriol. 186(2): (11) Van-Hoek AH, Mevius D, Guerra B, Mullany P, Roberts AP, Aarts HJ Acquired antibiotic resistance genes: an overview. Front. Microbiol. 2:203.

19 S7. Supplementary Table: Mutational analysis for rpob gene in different genomes of Acinetobacter baumannii. Genomes Reported rpob mutation AB1656 Human clinical specimens * Nd** AB17978 Human clinical specimens Nd AB0057 Human clinical specimens Nd ABACICU Human clinical specimens Nd ABMDRTJ Human clinical specimens Nd ABMDRZJ06 Human clinical specimens Nd AYE Human clinical specimens Nd TCDC-AB0715 Human clinical specimens Nd AB Human clinical specimens Nd ABSDF Lice Nd AB D Human clinical specimens Nd TYTH-1 Human clinical specimens Nd AB210 Human clinical specimens 1604 A-T AB900 Human clinical specimens Nd AB4190 Human clinical specimens Nd AB Human clinical specimens Nd ABAC12 Human clinical specimens Nd ABIC123 Human clinical specimens Nd ABNIH1 Human clinical specimens Nd ABNIH2 Human clinical specimens Nd ABNIH3 Human clinical specimens Nd ABNIH4 Human clinical specimens Nd ABNIH5 Human clinical specimens Nd ABNIH6 Human clinical specimens Nd ABNIH7 Human clinical specimens Nd ABNIH10 Human clinical specimens Nd ABNIH11 Human clinical specimens Nd ABNIH13 Human clinical specimens Nd ABNIH14 Human clinical specimens Nd ABNIH15 Human clinical specimens Nd ABNIH16 Human clinical specimens Nd ABNIH17 Human clinical specimens Nd ABNIH18 Human clinical specimens Nd ABNIH19 Human clinical specimens Nd ABNIH20 Human clinical specimens Nd ABNIH22 Human clinical specimens Nd ABNIH23 Human clinical specimens Nd ABNIH24 Human clinical specimens Nd ABNIH25 Human clinical specimens Nd ABNIH26 Human clinical specimens Nd ABNIPH24 - Nd ABNIPH60 - Nd ABNIPH67 - Nd ABNIPH70 - Nd

20 ABNIPH80 - Nd ABNIPH146 - Nd ABNIPH190 - Nd ABNIPH201 - Nd ABNIPH290 - Nd ABNIPH329 - Nd ABNIPH335 - Nd ABNIPH527 - Nd ABNIPH528 - Nd ABNIPH601 - Nd ABNIPH615 - Nd ABNIPH Nd ABNIPH Nd ABNIPH Nd ABNIPH Nd ABPR07 Human clinical specimens Nd AB-ANC4097 Human clinical specimens Nd AB515-8 Human clinical specimens Nd AB Human clinical specimens Nd Ab_15_45 Bronchoalveolar lavage Nd AB Human clinical specimens Nd ABA118 Human clinical specimens Nd ABIS116 Hospital Isolate Nd AB056 Human clinical specimens Nd ABIS235 Hospital Isolate Nd AB056 Human clinical specimens Nd AB059 Human clinical specimens Nd AB Human clinical specimens Nd AB Human clinical specimens Nd AB Human clinical specimens Nd AB Human clinical specimens Nd AB Human clinical specimens Nd AB Human clinical specimens Nd AB Human clinical specimens Nd AB Human clinical specimens Nd AB1H8 - Nd AB3909 Human clinical specimens Nd AB3990 Human clinical specimens Nd AB4190 Human clinical specimens Nd AB4857 Human clinical specimens Nd AB4A3 - Nd AB5075 Human clinical specimens Nd AB5256 Human clinical specimens Nd AB53264 Human clinical specimens Nd AB Human clinical specimens Nd AB Human clinical specimens Nd AB Human clinical specimens Nd AB_ Hospital surface Nd

21 AB_ Hospital surface Nd AB_ Hospital surface Nd AB Hospital sputum Nd AB Human clinical specimens Nd AB Nd AB Hospital surface Nd AB Tracheal aspirate Nd AB Human clinical specimens Nd AB Bronchoalveolar lavage Nd AB Nd AB Human clinical specimens Nd AB Human clinical specimens Nd AB Human clinical specimens Nd AB-TG Nd AB-AA014 - Nd AB-405E4 - Nd AB-ISacColiR Human clinical specimens Nd AB-ISacColiS Human clinical specimens Nd AB-IS58 Hospital Isolate Nd AB-6976 Human clinical specimens Nd AB-TG2018 Human clinical specimens 1565 A-T AB-TG2022 Human clinical specimens Nd AB-TG2023 Human clinical specimens Nd AB-TG Nd AB-TG2030 Human clinical specimens Nd AB-TG2632 Human clinical specimens Nd AB-TG5064 Human clinical specimens Nd AB-TG Nd AB-TG27327 Human clinical specimens Nd AB-TG27331 Human clinical specimens Nd AB-TG Nd AB-TG27339 Human clinical specimens Nd AB-TG27343 Human clinical specimens Nd AB Hospital surface Nd AB Hospital Isolate Nd AB Hospital Isolate Nd AB Hospital Isolate Nd AB-W7282 Human clinical specimens Nd AB-WC136 Health care equipment surface Nd AB-WC141 Health care equipment surface Nd AB-WC323 Health care equipment surface Nd AB-WC348 Human clinical specimens Nd AB-WC487 Human clinical specimens Nd AB-WC692 Human clinical specimens Nd AB-WC-A-92 Human clinical specimens Nd AB-WC-A-694 Human clinical specimens Nd AB-ZWS1122 Human Nd AB-WC-A-157 Environmental isolate Nd

22 AB-ZWS1219 Human clinical specimens Nd AB-OIFC021 Human clinical specimens Nd AB-OIFC032 Human clinical specimens Nd AB-OIFC035 Human clinical specimens Nd AB-OIFC047 Human clinical specimens Nd AB-OIFC065 Human clinical specimens Nd AB-OIFC074 Human clinical specimens Nd AB-OIFC087 Human clinical specimens Nd AB-OIFC098 Human clinical specimens Nd AB-OIFC099 Human clinical specimens Nd AB-OIFC0109 Human clinical specimens Nd AB-OIFC0110 Human clinical specimens Nd AB-OIFC0111 Human clinical specimens Nd AB-OIFC0137 Hospital Isolate Nd AB-OIFC0143 Human clinical specimens Nd AB-OIFC0162 Human clinical specimens Nd AB-OIFC0180 Navy Ship Nd AB-OIFC0189 Human clinical specimens Nd AB-OIFC0338 Human clinical specimens Nd AB-TG19582 Human clinical specimens Nd AB-UMB001 Human clinical specimens Nd AB-UMB002 Human clinical specimens Nd AB-UMB003 Human clinical specimens Nd AB-AC12 Hospital surface Nd AB-AC30 Hospital surface Nd AB-ATCC19606 Human clinical specimens Nd AB-BC1 Hospital Isolate Nd AB-BC5 Hospital Isolate Nd AB-IS116 Hospital Isolate Nd ABBZICU-2 Hospital Isolate Nd AB-IS235 Hospital Isolate Nd AB-IS143 Hospital Isolate Nd AB-IS251 Hospital Isolate Nd AB-MSP4-16 Soil Nd AB-NAVAL13 Human clinical specimens Nd AB-NAVAL17 Human clinical specimens Nd AB-NAVAL57 Human clinical specimens Nd AB-NAVAL72 Hospital Isolate Nd AB-NAVAL78 Human clinical specimens Nd AB-NAVAL81 Human clinical specimens Nd AB-NAVAL82 Human clinical specimens Nd AB-NAVAL83 Human clinical specimens Nd AB-TG2028 Human clinical specimens Nd AB-TG2030 Human clinical specimens Nd AB-TG2031 Human clinical specimens Nd *The sample isolated from blood, skin, wound, urine etc from humans. ** Nd- not detected