Mini Gel Tank. Versatility. Easy loading. Enhanced visualization.

Transcription

1 Mini Gel Tank Versatility. Easy loading. Enhanced visualization.

2 2 Invitrogen Mini Gel Tank

3 W E S T E R N W O R K F L O W SEPARATE TRANSFER DETECT One tank, more gel choices Discover the convenience of our new Mini Gel Tank Introducing the Invitrogen Mini Gel Tank. We ve taken our Invitrogen olt Mini Gel Tank designed for more intuitive use and convenience than traditional electrophoresis tanks and modifi ed it to provide increased fl exibility. Now you can run your favorite Invitrogen NuPAGE, Novex, and olt gels using the same tank. The new Mini Gel Tank offers: Versatility compatible with NuPAGE, Novex, and olt mini gels Easy sample loading forward-facing well confi guration Simultaneous visualization of both gels streamlined, side-by-side tank confi guration Simple monitoring of prestained protein markers with white tank stand Less running buffer required two separate gel chambers, so you only need to load suffi cient buffer for each gel to the specifi ed fi ll line High performance same great results you ve been getting with the Invitrogen XCell SureLock Mini-Cell and original olt Mini Gel Tank thermofi sher.com/minigeltank 3

4 Are you an Invitrogen olt system user? If you re already using a olt Mini Gel Tank, learn how you can upgrade your tank to experience the same versatility as the new Mini Gel Tank. We ll provide you with a new Invitrogen Cassette Clamp Cam Handle Set for free, so you can easily upgrade your original olt Mini Gel Tank to be compatible with NuPAGE, Novex, and olt mini gels. olt mini gels have also been redesigned for your convenience, to fi t into the updated Mini Gel Tank with the new cam handles. Equal performance is observed with the original olt gels and the new olt gels (Figure 1). Upgrade your olt tank Watch this video to learn how to enhance the fl exibility of your olt Mini Gel Tank with your new Cassette Clamp Cam Handle Set (free of charge for olt tank users). Go to thermofisher.com/bolttanksupport Enhanced versatility of the Mini Gel Tank Original olt Mini Gel Tank with original cam handles New Mini Gel Tank (or enhanced olt tank with replacement cam handles) Easy sample loading with forward-facing well confi guration Minimal running buffer required Side-by-side simultaneous visualization of 2 gels Simple monitoring of pre-stained protein markers against a white tank stand Compatible mini protein gels Original olt is-tris Plus gels (10.5 cm) New olt is-tris Plus gels (10 cm) NuPAGE is-tris gels NuPAGE Tris-Acetate gels Novex WedgeWell Tris-Glycine gels Novex Tricine gels 4 Invitrogen Mini Gel Tank

5 A Redesigned olt gel, new Mini Gel Tank Original olt gel, olt Mini Gel Tank Figure 1. Equal performance of a new olt 4 12% is-tris Plus gel vs. original olt gel with protein standards and samples. The same protein standards and samples were loaded (10 μl each) in a new olt gel and an original olt gel. Similarly sharp, straight bands with very similar migration were observed on (A) the new olt gel and () the original olt gel. Lane 1: Invitrogen Novex Seelue Plus2 Pre-stained Standard; lane 2: 10 μg E. coli lysate; lane 3: blend of 12 purified proteins (Invitrogen Novex Mark12 Unstained Standard); lane 4: 40 μg HeLa cell lysate; lane 5: 20 μg HeLa cell lysate; lane 6: 5 μg SA; lane 7: 40 μg Jurkat cell lysate; lane 8: 5 μg of a GST fusion protein; lane 9: Invitrogen Novex Sharp Protein Standard; lane 10: 5 μg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using Invitrogen Novex Simplylue SafeStain, and images were acquired using a flatbed scanner. W E S T E R N W O R K F L O W SEPARATE TRANSFER DETECT Are you a NuPAGE or Novex gels user? As a user of NuPAGE or Novex gels, you appreciate the high-quality results these products offer you. Now, for the first time, these mini protein gels can be used with our Mini Gel Tank. Switching from your current tank to the Mini Gel Tank provides you with several advantages, including more gel choices, easy loading, and enhanced visualization now see bands more clearly than ever before. In the following comparisons, you can see the equivalent performance of Invitrogen NuPAGE Novex is-tris (Figure 2), NuPAGE Novex Tris-Acetate (Figure 3), Novex WedgeWell Tris-Glycine (Figure 4), and Novex Tricine (Figure 5) gels in the new Mini Gel Tank, as compared to their performance in the XCell SureLock Mini-Cell. thermofisher.com/minigeltank 5

6 A NuPAGE is-tris gel, Mini Gel Tank NuPAGE is-tris gel, XCell SureLock Mini-Cell Figure 2. Equal performance of NuPAGE is-tris gels with protein standards and samples, using a Mini Gel Tank or the XCell SureLock Mini-Cell. The same protein standards and samples were loaded (10 µl each) in NuPAGE 4 12% is-tris gels. Electrophoresis was performed using either the Mini Gel Tank or the XCell SureLock Mini-Cell. Similarly sharp, straight bands with very similar migration were observed when using (A) the Mini Gel Tank and () the XCell SureLock Mini-Cell. Lane 1: Seelue Plus2 Pre-stained Standard; lane 2: 10 μg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12 Unstained Standard); lane 4: 40 μg HeLa cell lysate; lane 5: 20 μg HeLa cell lysate; lane 6: not used; lane 7: 40 μg Jurkat cell lysate; lane 8: 5 μg of a GST fusion protein; lane 9: Novex Sharp Protein Standard; lane 10: 5 μg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using Simplylue SafeStain, and images were acquired using a flatbed scanner. A NuPAGE Tris-Acetate gel, Mini Gel Tank NuPAGE Tris-Acetate gel, XCell SureLock Mini-Cell Figure 3. Equal performance of NuPAGE Tris-Acetate gels with protein standards and samples, using a Mini Gel Tank or the XCell SureLock Mini-Cell. The same protein standards and samples were loaded (10 µl each) in NuPAGE 3 8% Tris-Acetate gels. Electrophoresis was performed using either the Mini Gel Tank or the XCell SureLock Mini-Cell. Similarly sharp, straight bands with very similar migration were observed when using (A) the Mini Gel Tank and () the XCell SureLock Mini-Cell. Lane 1: Seelue Plus2 Pre-stained Standard; lane 2: 10 μg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12 Unstained Standard); lane 4: 40 μg HeLa cell lysate; lane 5: 20 μg HeLa cell lysate; lane 6: 5 μg SA; lane 7: 40 μg Jurkat cell lysate; lane 8: 5 μg of a GST fusion protein; lane 9: Novex Sharp Protein Standard; lane 10: 5 μg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using Simplylue SafeStain, and images were acquired using a flatbed scanner. 6 Invitrogen Mini Gel Tank

7 A Novex WedgeWell Tris-Glycine gel, Mini Gel Tank Novex WedgeWell Tris-Glycine gel, XCell SureLock Mini-Cell Figure 4. Equal performance of Novex WedgeWell Tris-Glycine gels with protein standards and samples, using a Mini Gel Tank or the XCell SureLock Mini-Cell. The same protein standards and samples were loaded (10 µl each) in 4 20% Tris-Glycine gels. Electrophoresis was performed using either the Mini Gel Tank or the XCell SureLock Mini-Cell. Similarly sharp, straight bands with very similar migration were observed when using (A) the Mini Gel Tank and () the XCell SureLock Mini-Cell. Lane 1: Seelue Plus2 Pre-stained Standard; lane 2: 10 μg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12 Unstained Standard); lane 4: 40 μg HeLa cell lysate; lane 5: 20 μg HeLa cell lysate; lane 6: 5 μg SA; lane 7: 40 μg Jurkat cell lysate; lane 8: 5 μg of a GST fusion protein; lane 9: Novex Sharp Protein Standard; lane 10: 5 μg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using Simplylue SafeStain, and images were acquired using a flatbed scanner. A Novex Tricine gel, Mini Gel Tank Novex Tricine gel, XCell SureLock Mini-Cell Figure 5. Equal performance of Novex Tricine gels with protein standards and samples, using a Mini Gel Tank or the XCell SureLock Mini-Cell. The same protein standards and samples were loaded (10 µl each) in 10 20% Tricine gels. Electrophoresis was performed using either the Mini Gel Tank or the XCell SureLock Mini-Cell. Similarly sharp, straight bands with very similar migration were observed when using (A) the Mini Gel Tank and () the XCell SureLock Mini-Cell. Lane 1: Seelue Plus2 Pre-stained Standard; lane 2: 10 μg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12 Unstained Standard); lane 4: 40 μg HeLa cell lysate; lane 5: 20 μg HeLa cell lysate; lane 6: 5 μg SA; lane 7: 40 μg Jurkat cell lysate; lane 8: 5 μg of a GST fusion protein; lane 9: Novex Sharp Protein Standard; lane 10: 5 μg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using Simplylue SafeStain, and images were acquired using a flatbed scanner. thermofisher.com/minigeltank 7

8 Choose from a variety of mini protein gels compatible with the new Mini Gel Tank. If you currently own an original olt Mini Gel Tank, contact customer service to request a no-charge replacement set of cam handles (Cat. No. A26732) to upgrade your tank. Ordering information Product Quantity Cat. No. Mini Gel Tank 1 unit A25977 Cassette Clamp Cam Handle Set, 10 cm 1 set A26732 olt is-tris Plus Gels Varies Multiple NuPAGE is-tris Gels Varies Multiple NuPAGE Tris-Acetate Gels Varies Multiple Novex WedgeWell Tris-Glycine Gels Varies Multiple Novex Tricine Gels Varies Multiple Find out more at fishersci.com In the United States: For customer service, call To fax an order, use To order online: fishersci.com In Canada: For customer service, call To fax an order, use To order online: fishersci.ca For Research Use Only. Not for use in diagnostic procedures Thermo Fisher Scientifi c Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientifi c and its subsidiaries unless otherwise specifi ed. N