Reach greater highs. (and lows ) with new protein ladder choices. Fermentas now sold as. Thermo Scientific

Transcription

1 Introducing Thermo Scientific Pierce Prestained and Unstained s for easy protein molecular weight determination. Fermentas now sold as Thermo Scientific Reach greater highs (and lows ) with new protein ladder choices.

2 Fermentas now sold as Thermo Scientific The Thermo Scientific Pierce Protein Research Portfolio now includes protein ladders made with Fermentas technology. The Prestained s are recommended for approximate determination of molecular weight, monitoring the progress of the electrophoresis run and the efficiency of the protein transfer to the membrane during Western blotting procedures. For precise determination of molecular weights of target proteins in any buffer system, we recommend using our unstained protein ladders. Highlights: Cost effective high quality without the high price Performance sharp protein band resolution and consistent migration patterns provide easy molecular weight determination Convenient protein ladders are ready to load with no heating or boiling required Reliable exceptional lot-to-lot consistency and reproducibility new protein ladder choices

3 Reach new heights in gel electrophoresis Ordering Information # PI-66 PI-661 PI-6614 PI-6616 PI-6617 PI-6619 PI-6 PI-6634 PI-663 PI-66 PI-668 PI-66 PI-663 PI-6637 Description Pierce Unstained Protein MW Marker Pierce Prestained Protein MW Marker PageRuler Unstained PageRuler Plus Prestained PageRuler Plus Prestained Spectra Multicolor Broad Range Broad Range High Range Low Range Broad Range Low Range High Range Pkg. Size x 1mL 0µL x 0µL x 0µL x 0µL x 0µL x 0µL x 0µL x 0µL x 0µL 0µL x 0µL x 0µL x 0µL Specialty Ladders # PI-66 PI-8478 PI Description NIR SuperSignal Molecular Weight SuperSignal Enhanced Molecular Weight Pkg. Size x 0µL 0μL 0μL Bulk-size Packages Available Visit to request a quote, or contact our Bulk and Custom Sales department at LVC.rockford@thermofisher.com.

4 Low Range Low Range Unstained Ladder Prestained Ladder Pierce Unstained Marker Pierce Prestained Marker Ladder Ladder PageRuler Plus Prestained Ladder Broad Range Broad Range High Range High Range Range () Application Thermo Scientific Pierce s SDS PAGE Western blotting Colorimetric Unstained Prestained Chemi. Lum. NIR Name # Broad Range Low Range High Range Pierce Unstained Protein PageRuler Plus Prestained Broad Range Low Range High Range Pierce Prestained Protein SuperSignal Molecular Weight NIR Former Fermentas Part # # Proteins MW Range Reference Bands PI-66 SM , and 0 Best Best Good PI-6614 SM Good Good Good PI-663 SM Best Best Good PI Best Best Good PI-66 SM PI-6616, PI-6617 PI-6619, PI-6 PI-6634, PI-663 SM0671, SM067 SM1811, SM181 SM1841, SM184-1 Green ; Orange 9-0 Green ; Orange and - Green and ; Orange, and ; Pink 1 PI-668 SM Green ; Orange PI-66 SM Green ; Orange and 0 Good Good Good Good Good Good Good Good Good Good Good Good Good Best Best Good Best Good Best Best Good Best Good Best Best Good Best PI-661 SM Good Good Good Good Good PI-8478, PI Protein MW determination Protein band visualization Monitoring electrophoresis Good Good Good Best PI Good Good Good Best Good run NIR detection Coomassie/silver/ fluorescent staining Monitoring protein transfer Chemiluminescent band visualization with Strep-Tactin HRP or AP Conjugates Blue and green prestained bands fluoresce in NIR region

5 Reference Guide: Migration Patterns in Different Electrophoresis Conditions Thermo Scientific concentration 4-0% 8-16% -0% % 1% % Migration Patterns of PageRuler Prestained type % Apparent Molecular Sizes () % 1 1 1% concentration 4-0% % 1% % % Apparent Molecular Sizes () % % Thermo Scientific Broad Range type concentration 4-0% 8-16% -0% % 1% % % Apparent Molecular Sizes () % % % % 4-0% 3- Apparent Molecular Sizes () Thermo Scientific Low Range type 4-1% concentration 4-0% -0% % Tris-Tricine 14-SP % 16-SP % 18-SP % -0% Tris-Tricine SDS Apparent Molecular Weights () 1 16% -0% 14% Note: The apparent molecular size of each protein () has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Migration patterns were determined using respective NuPAGE precast gels. 1% Thermo Scientific High Range type concentration 4-1% Migration Patterns of Broad Range -0% 0 Migration Patterns of High Range and Low Range 8-16% Migration Patterns of PageRuler Plus Prestained Thermo Scientific PageRuler Plus Prestained type 0 SP small peptide SDS-polyacrylamide gel with higher degree of crosslinking (c=% instead of usual ~3%). migration patterns were determined using respective Novex precast gels. migration patterns were determined using respective Ready precast gel.

6 Thermo Scientific Pierce Protein Thermo Scientific s The Thermo Scientific Pierce Prestained Protein is designed to monitor protein migration during SDS-polyacrylamide gel electrophoresis and monitor protein transfer onto membranes. Pierce Unstained Protein Molecular Weight Marker produces sharp bands on SDS-polyacrylamide gel following staining with Coomassie-based or silver stains and is recommended for accurate sizing of proteins on SDS PAGE and Western blots. The Thermo Scientific PageRuler and PageRuler Plus Prestained s are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination. The green and orange reference bands in the PageRuler s provide easy orientation of the protein bands. Protein ~1 ~ ~ 8 ~1 66. Bovine serum albumin ~ 18.4 β-lactoglobulin 14.4 Lysozyme ~0 ~ ~ ~.0 REase Bsp98I ~ ~0 ~.0 Lactate dehydrogenase ~ ~1 ~0 4.0 Ovalbumin ~ ~0 ~ ~ ~ ~ ~ ~ ~ 8-16% Tris-glycine 8-16% Tris-glycine 4-0% Tris-glycine 4-0% Tris-glycine # #PI ##PI , 6617 #s #s PI-6616, PI , 6 #s #s PI-6619, PI-6 Thermo Scientific s Thermo Scientific s Thermo Scientific s are mixtures of recombinant, highly purified proteins designed for accurate sizing of proteins by. The ladders resolve into clearly identifiable sharp bands when analyzed by and stained with Coomassie Blue or silver stain. Each ladder contains protein bands of greater intensity that serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S, or Coomassie Blue (except for 3.4 and polypeptides) or by using Strep-Tactin conjugates. Four different chromophores bound to proteins in Thermo Scientific Spectra Multicolor Broad Range s produce a brightly colored ladder with an easy-to-remember pattern. The High Range and Low Range s are designed for large and small protein analysis, respectively ~0 0 ~ ~ ~ ~ ~ 4.6 ~ 1.7 #s 663, PI #s PI-663, 4-0% Tris-glycine # #PI Thermo Scientific SuperSignal Molecular Weight s contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 0 to 0. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. Use SuperSignal Enhanced Molecular Weight s with mouse monoclonals. µl µl % Bis-Tris The NIR is a mixture of proteins (11 to 0) that are bluestained and fluor-labeled for near-ir fluorescent visualization and protein sizing. The protein MW markers in this ladder resolve into sharp bands when analyzed by and are labeled with a fluorescent dye for visualization with instruments equipped with near-infrared (NIR) fluorescence detection. The bands are also directly visible because the proteins are prestained blue. 4-0% #s PI-8478, PI #s 8478, Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Novex and NuPAGE are registered trademarks of Invitrogen. Ready is a registered trademark of Bio Rad. Strep-Tactin is a registered trademark of IBA GmbH. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. In the United States: For customer service, call To fax an order, use To order online: In Canada: For customer service, call To fax an order, use To order online: ~ ~ 4-1% Tris-glycine # #PI # PI-668 # 668 Thermo Scientific NIR SuperSignal Molecular Weight ~0 1SP SPTris-tricine Tris-tricine 1 - Small Peptide SP -SP Small Peptide SDS SDS - polyacrylamide gel polyacrylamide gel with with higher crosslinking, C=% higher crosslinking, C=% # #PI µl µl ~1 ~ 4-0% Tris-glycine Tris-glycine 4-0% 1 SP Tris-tricine Thermo Scientific SuperSignal Molecular Weight s ~ ~1 ~ ~ 3.4 ~0 ~0 ~ ~ PageRuler NIR Near-IR Imager # #PI ~1 ~ 0 8 High Range Visible Light 4-0% Tris-glycine 0 Low Range X-ray film High Range Broad Range 0 0 ##PI % Tris-glycine Low Range Broad Range PageRuler Unstained BN014 04/1 Printed in the U.S. ~ β-galactosidase PageRuler Plus Prestained Unstained Protein Prestained Protein 4-0% Tris-glycine ##PI-66 66