Technische Universität München. Early blight subgroup

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1 Early blight subgroup

2 summarise protocols

3 18 different lab protocols + qpcr + Artificial inoculation + Long-Term Storage + Growth and conidia production + Isolation + Characterization of Cytb mutations

4 protocols: download EUROBLIGHT homepage

5 subgroup meeting March 2014 in Munich

6 subgroup meeting March 2014 in Munich

7 subgroup meeting March 2014 in Munich: Title of presentation Results of field trials with fungicide timing Monitoring and field experiments with Early Blight in Denmark Protocol for testing fungicides, results of field trials 2014 Fungicide rating new approach Pathogenicity of Alternaria species: Summary of BASF results Author Jan Spoelder Bent Nielsen Hans Hausladen Bert Evenhuis Vanessa Tegge Genotype distribution and strobilurin sensitivity of Alternaria solani in Sweden Eva Edin Occurrence of F 129 L mutation of A. solani in Germany Mutation of A. alternata Alternaria solani & ozone Birgit Adolf Jan Nechwatal Jan Spoelder Towards a knowledge-based approach for an integrated control of Alternaria spp. in potatoes Sofie Landschoot

8 : subgroup meeting March 2014 in Munich: Diagnostic ring test

9 Background Alternaria ring test Collection of Alternaria infected leaves from different countries Shipment to different labs, sample preparation and evaluation of Alternaria species Participating Labs: Bert Evenhuis and Marieke Förch (WUR, The Netherlands) Jan Spoelder (HLB, The Netherlands) Jürgen Leiminger and Anna Livic (TUM, Germany) each lab will analyze leaf samples according to their own lab protocols analysis of Alternaria species according to spore formation (microscope)

10 analysed leaf samples Nr. country sample sent by field location leaf level variety fungicide treatment disease severity sampling date 1 Germany Jürgen Freising low-middle Agila untreated? Germany Jürgen Freising low-middle Maxilla untreated? Germany Jürgen Straßmoos middle unknown Mancozeb moderate Belgium Pieter Passionistenstraat, Kruishoutem 9770 middle Bintje untreated 5% The Netherlands Jan Wijster? Festien untreated 10% Sweden SLU Nymö? Kuras untreated? Sweden SLU Nymö? Kardal untreated? Sweden SLU Nymö? Kuras untreated? Germany Jürgen Niedersunzing high unknown treated moderate to high Belgium Pieter Passionistenstraat, Lozer Middle-high Bintje untreated 0.5-1% The Netherlands Marieke Westmaas higher unknown treated? The Netherlands Marieke Valthermond Aveka untreated 20% The Netherlands Marieke Lelystad higher Bintje untreated 75%

11 first country report on early blight :

12 : first country report on early blight 2013 & 2014: + disease / epidemic outbreak + disease severity + fungicide used + Alternaria species + yield loss + early blight model: infection risk (Phytophthora model Weihenstephan)

13 Early blight model: calculate infection risk for 28 EU weather station Bonin, PL, 2014 Alnarp, SE, 2013

14 subgroup meeting March 2014 in Munich: Decimal rating of EB fungicides harmonised protocol for testing eb fungicides

15 new objective of the EB sub group basis (valid/actual data base) for a IPM system to control EB (e.g. isolate characterisation, fungicide sensitivity, )

16 new initiatives Monitoring F129L mutation in A. solani in Europe

17 new initiatives: lab involved in the F129L Monitoring 6 labs: Alison Erland Bent Bert Pieter Birgit

18 Interaction with the international networks Share informations: e.g. protocols, fungicide efficacy, fungicide sensitivity.

19 Thanks to Early blight subgroup