Symphony X. Multiplex Peptide Synthesizer. The World s Leading Manufacturer of Peptide Synthesizers
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1 Symphony X Multiplex Peptide Synthesizer The World s Leading Manufacturer of Peptide Synthesizers
2 2 The Symphony X Peptide Synthesizer Maximum Throughput & Production Fast, Efficient Couplings High Quality Crude Peptides Extremely Flexible Automated Cleavage 12 Independent Reaction Pairs mmol scales Up to 12 RV s with pre-activation Up to 24 RV s with in situ activation Adjustable nitrogen mixing Independent operation Top-down washing Optional IR heating &/or UV-monitoring capability in RV position 1 Automated Cleavage 50 ml collection tube capacity 12 positions may alternatively be used as Single-ShotTM AA bottles Dedicated TFA bottle position Customizable cleavage programs Adjustable start times VGA 17 Touchscreen Full color Resistant to all synthesis chemicals Flexible, easy-to-use software No external computer! 28 Amino Acid Positions 10, 120 & 400 ml sizes available Single-ShotTM deliveries Pre-packed amino acid bottles available Sensor deliveries Automated Integrated Solvent Cabinet 8 Solvent Positions Maximize flexibility 20 L solvent container Sensor deliveries Automated cleaning routines Enclosed, ventilated & easily accessible Floor-standing model with wheels easily fits in your lab Chemical spill trays for spill containment Pull out drawer for convenient storage
3 3 High Quality Performance High Purity Crude Peptides ACP (H-VQAAIDYING-OH) Choose Between In Situ & Pre-activation In situ activation can be accomplished by delivering amino acid to the reaction vessel first, followed by activator AA ACT REACTION VESSEL C-peptide (H-EAEDLQVGQV ELGGGPGAGSLQ PLALEGSLG-OH) Top row reaction vessels may be used as pre-activation chambers for bottom row reaction vessels. AA ACT TOP ROW PREACTIVATION CHAMBER BOTTOM ROW REACTION VESSEL Unbeatable Throughput! 24 reaction vessels gives you 24 times the throughput of any serial peptide synthesizer! When it comes to custom peptide production, nothing can match the Symphony X! Twenty-four 42-mers in 14 hours! Twenty-four 68-mers in 25 hours! Purified Peptide Crude Peptide Purified Peptide Crude Peptide β-amyloid 1-42 Chemokine SDF-1a (H-DAEFRHDSGY EVHHQKLVFF AEDVGSNKGA IIGLMVGGVV IA-OH) (H 2 N-KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK-OH) Deprotection Time: 2 min Deprotection Time: 4 min Coupling Time: 5 min Coupling Time: 5 min Cycle Time: 21 min Cycle Time: 23 min Total Synthesis Time, 24 Peptides: 14 hours! Total Synthesis Time, 24 Peptides: 25 hours!
4 4 Run different sequences, scales and protocols on multiple reactors all at the same time! Start, Stop or Pause reactors whenever you wish! It s like having 12 synthesizers in one! Each reaction vessel pair can: Run Any Program Run Any Scale Run Any Reagent Run Any Reaction Time Start/Stop/Pause regardless of what the other reaction vessels are doing! Innovative Features 12 Synthesizers in One! Robust, Reliable Construction Preactivation Now you can preactivate on multiple reactors in parallel. Perfect for sterically hindered amino acid additions! Optimize your DIC/HOBt chemistry on the Symphony X and go straight to scale-up! Special Reagent Additions Other systems use off-the-shelf valves that leak, cross-contaminate, and quickly fail when exposed to harsh peptide synthesis chemicals. Our patented valve block system was specifically designed for peptide synthesis, so you get zero dead volumes, no crosscontamination, and worry-free performance that lasts for years! 10 ml Single-Shot Amino Acid Vial At last! Add expensive special reagents to any reactor without priming or wasting a drop using Single-Shot deliveries! Save money with no manual intervention required! The Symphony X comes with up to 40 amino acid positions to accommodate any chemistry!
5 5 Infrared (IR) Heating Notification Monitor the status of your synthesis from your: Computer Cell Phone Faster than microwaves Test the same protocol with and without heat in the same synthesis! Vortex mixing ensures no hot spots! Most stable heating system on the market today - doesn t overshoot! Highly accurate temperature sensing for precise temperature control Minimize process variables during scale-up UV-Monitoring Choose to be notified: At the beginning of each cycle When an error occurs At the end of a synthesis Safe-Response Feature Keep your reactions safe during unexpected interruptions. When your synthesis is interrupted for any reason, the Symphony X will: Drain active reaction vessels Wash resin with solvent of your choice Prevent side reactions and undesired byproducts Minimize trial-and-error when synthesizing difficult peptides using real-time UVmonitoring on the Symphony X. What makes our UV-monitoring different? Other systems just monitor the waste stream. We monitor your reaction solution while it s mixing that s why we re the only ones who can control the deprotection reaction times, not just the number of repetitions! Automated Cleavage* The Symphony X features automated cleavage. Unlike some peptide synthesizers, all PTI synthesizers are built with robust TFAresistant materials, so you can cleave for years on our instruments without worry. Not interested in cleavage? You can also use the Symphony X collect positions as additional amino acid positions! *Note: Fmoc chemistry only
6 6 Flexible, Easy-To-Use Software Create, view or edit programs Select a sequence(s) and assign a program to each cycle Load synthesis and monitor progress of all reactions All With Easy Touchscreen Programming Other Software Features Automated cleaning routines Waste level detection Manual operations Customizable amino acid and solvent tables Calculations screens Synthesis record Good Laboratory Practice data recording Diagnostics and error reporting Flexible restart options for interrupted syntheses
7 7 Accessories, Reagents & Service Accessories 10 ml Reaction 10 ml Glass Reaction 10 ml Frosted Glass Reaction 45 ml Reaction 40 ml Glass Reaction 40 ml Frosted Glass Reaction Cat#: PPS-R10-030, 30 Cat#: PPS-R10-090, 90 Cat#: PPS-R10-180, 180 Cat#: TPS-GRV10-1, 1 ea. Cat#: TPS-GRV10-10, 10 Cat#: TPS-FGRV10-1, 1 ea. Cat#: TPS-FGRV10-10, 10 Cat#: PPS-R45-030, 30 Cat#: PPS-R45-090, 90 Cat#: PPS-R45-180, 180 Cat#: TPS-GRV40-1, 1 ea. Cat#: TPS-GRV40-10, 10 Cat#: TPS-FGRV40-1, 1 ea. Cat#: TPS-FGRV40-10, ml Single-Shot TM AA Bottle Cat#: AAR-SSI, 1 ea. Cat#: AAR-SSX, ml AA Bottle Cat#: SMP-VX-020, 20 Cat#: SMP-VX-100, ml AA Bottle Cat#: AAR-400I, 1 ea. Cat#: AAR-400X, ml Collect Vial Cat#: CLV , 30 PTI Service and Support In addition to supplying superior instrumentation, PTI offers the finest in technical support and field service. Our service team specializes in one thing - supporting PTI synthesis instruments. From installation to preventative maintenance, our responsive field service group will make sure that you get the most out of your investment. PTI offers varying levels of service agreements Please contact PTI for terms and pricing Use PTI Reagents After choosing the right synthesizer, nothing is more important to your synthesis success than the quality of the reagents used with your instrument. Use PTI brand amino acids to maximize the performance of your automated peptide synthesizer. Our amino acids are quality screened and optimized for use on PTI instruments to help ensure the production of high quality peptides and the reliable operation of your instrument, and come in affordable pre-weighed bottles for your convenience as well as in bulk. Specifications for PTI products and services on all pages in this publication are subject to change without notice. PS3 is a trademark and Overture, Symphony, Prelude, Tribute and Sonata are registered trademarks of Protein Technologies, Inc. PTI products are manufactured under U.S. Patent 5,203,368, other U.S. and international patents issued and pending. Copyright 2013 Protein Technologies, Inc. All rights reserved. DOC # Rev
8 Symphony X Specifications Number of reaction vessels... Synthesis scale range... Reaction vessel volume... Number of solvent positions/sizes... Number of amino acid positions... Chemistries supported... Activation... Throughput... Fluid measurement technique... Fluid transfer method... Agitation method... Cleavage... Waste container... Control interface... Reporting... Power (12 with pre-activation) mmol to 22.4 mmol (up to ~2 g of resin per RV) Plastic, disposable - 10 ml and 45 ml Glass - 10 ml and 40 ml 1 primary solvent position, 20 L capacity 7 user-defined solvent positions, 0.5 L - 4 L capacities Up to 40 amino acid positions 10, 120 or 400 ml bottles Fmoc, t-boc, organic, peptoid, combinatorial, others DIC/HOBt, HBTU, HATU, HCTU, TBTU, PyBOP, others User defined Fixed loop measurement with optical sensors Positive pressure with nitrogen Nitrogen bubbling &/or vortex mixing - adjustable and programmable Automatic, programmable (for Fmoc chemistry only) 20 L container with overflow sensor Proprietary Symphony X control software Internal computer and touchscreen monitor included Real-time log of each instrument function, print to file, screen or printer. 115 V/60 Hz or 230 V/50 Hz Dimensions W, 29 D, 66 H (114 cm W, 74 cm D, 168 cm H) Weight... Warranty... Optional lbs. (250 kg) One year, parts and labor Infrared (IR) Heating (SYMX-IR-OPT) UV-Monitoring (SYMX-UV-OPT) /Text Message Status Reporting (SYMX-TXT-EML-RPT) Ordering Information: Catalog # SYMX-110 SYMX-230 Description Symphony X Peptide Synthesizer, 115 V Symphony X Peptide Synthesizer, 230 V For more information: info@ptipep.com Web: Main Office: 4675 South Coach Drive Tucson, AZ 85714, U.S.A. Phone: Toll Free: Fax:
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