Ph.D. Course Work Sub : Zoology
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1 Ph.D. Course Work 2012 Sub : Zoology Important points to be noted: Duration of Course Work : One Semester (6 Months) Total : 200 (Four papers 50 Each) Passing marks will be: 40 %. Duration of Examination: 2 Hrs. for each paper I: Research Methodology : 50 II: Subject paper -I : 50 III : Subject paper II : 50 IV: Review of literature : 50 Subject Course Work: 100 (2 Hrs.) Examination will be held at the end of the Semester. Total Lecture Hour (periods) for II (elective units): 60 (sixty). Scheme of Examination of Pre-Ph.D. (Course Work) Examination I Research Methodology II Subject I III Subject II IV Review of Literature
2 I Research Methodology Unit - 1 -I Research Methodology 1. Introduction to Research Methodology: Meaning, Objectives, Significance, Types 2. Research Problem: Definition, testing and procedures Unit-2 1. Hypothesis: Definition, testing and procedures 2. Research Design/Plan: Meaning, need and features, Basic principle of experimental signs Unit-3 1. Data collection/data analysis: Tools for data collection 2. Statistical Applications: Mean, Median, Mode, Mean Deviation & Standard deviation, Correlation and Regression, Probability distributions,chi square test, z test, t test, two sample t test, paired-t test Unit Report Writing: Types of Reports, Research Report Format, Referencing, Bibliography, Appendices 2. Computer Applications: Fundamentals of Computers, Operating systems, use of software (MS-Office, SPSS) Unit-5 1. Safety and safety measures: Introduction, safety of individuals/laboratory/community/environment 2. Code of Ethics/ethics of research/good laboratory practices: IPR and record keeping/archives References: 1. Methodology of Research in Social Sciences by O. R. Krishnaswamy and M. Rangnatham Himalaya publication House, 2005, ISBN: Research Methodology: Methods and Techniques by C. R. Kothari, New Age International Publishers, ISBN: Statistical Methods for Research Workers by Fisher R. A., Cosmo Publications, New Delhi ISBN: Design and Analysis of Experiments by Montogomery D.C. (2001), John Wiley, ISBN: SPSS online manual 6. MINITAB online manual
3 II Subject I PAPER II Unit I- Killing and Fixation, Collection and Preservation of Biological samples; Metachromasia, PAS, and Feulgen Staining Techniques, Collection and Maintenance of Embryos Unit II Different types of microscope (Light microscope, Compound microscope, dark field microscope, Phase contrast microscope, Normaski microscope, Confocal microscopy, Transmission electron microscopy (TEM) and scanning electron microscopy (SEM)), Cells sorting flow cytometry, Inverted microscope, Stereoscopic microscope. Processing methods in Microscopy Unit III Different types of Microtomes; Rocking, Rotary, Freezing microtome, Cryo-techniques, Ultra tomes Unit IV Centrifuges, High speed Centrifuges, Ultra Centrifuges. ph meters. Unit V Colorimeters, Spectrophotometer and Flame Photometer Balance Types; Electric and Electronic Balance
4 III Subject II PAPER III Unit I Chromatography techniques, Principles and different types (Affinity chromatography, Ionexchange chromatography, Gel-exclusion chromatography, Gas chromatography, HPLC, TLC, chromatography), Isolation of natural products (extraction, purifications and separation). Electrophoretic techniques, Iso-electrofocusing, 2D-gel, 2DIE. Unit II Electrophysiological equipments; Kymographic equipment and Oscilloscope, Patch clamp, Photomicrography. Unit III Radiation; Types of radiations, principles of autoradiography, scintillation and Gamma counter. Principles and working system of audio visual equipments. Unit IV Use of computers in Life science, Fundamental of computer architecture, computational methods, nucleic acid and protein sequence database, Web-based tools for sequence searches, Motif analysis and presentation. Unit V Histo-chemical and Immuno-techniques; Antibody generation, detection of molecules using ELISA, RIA, Western blot, Immuno-precipitation and Immuno-fluorescence microscopy, detection of molecules in living cells.
5 IV Review of Literature PAPER IV Review of literature.
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