Break the Mold with Rapid Yeast and Mould detection methods M. All Rights Reserved.

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1 Break the Mold with Rapid Yeast and Mould detection methods M. All Rights Reserved.

2 M. All Rights Reserved.

3 Enabling Customer Success: Our Five Market-Leading Businesses Groups Industrial From purification to aerospace changing how industry works Safety & Graphics From protecting people & information to enhancing visual & design communication Electronics & Energy Enabling tomorrow s lifestyle today with power, communications and electronics > Sales: $29.9 B > R&D investment $1.6 B > 3,100+ patents issued in 2012 > 40,500+ issued & pending patents Consumer From simplifying life at home to keeping you organized at work Health Care From preventing infections to making smiles brighter M. All Rights Reserved.

4 Ab Abrasives Ad Adhesives Am Advanced Materials Ce Ceramics Co Advanced Composites Do Dental & Orthodontic Materials Em Electronic Materials Materials Fi Films Fl Fluoro-materials Nt Nanotechnology Nw Nonwoven Materials Po Porous Materials & Membranes Sm Specialty Materials 46 Core Technology Platforms Successful New Product Growth Builds on Uncommon Connections Mo Molding Mr Micro-replication Pd Particle & Dispersion Processing Pe Predictive Engineering & Modeling Pm Polymer Processing Pp Precision Processing Processing Rp Radiation Processing Su Surface Modification Vp Vapor Processing An Analytical As Application Software Es Electronics & Software Fc Flexible Converting & Packaging In Inspection & Measurement Is Integrated Systems & Design Capabilities Pr Process Design & Control Se Sensors We Accelerated Weathering Ac Acoustic Control Bi Biotech Dd Drug Delivery Di Display Ec Energy Components Fe Flexible Electronics Fs Filtration, Separation, Purification Im Imaging Lm Light Management Applications Md Medical Data Management Mf Mechanical Fasteners Mi Microbial Detection and Control Op Opto-electronics Tt Track and Trace Wo Wound Management M. All Rights Reserved.

5 3M Innovation Model Technology Abrasives Platforms Adhesives Ceramics Electronics, Software Films Microreplication Nanotechnology Non-wovens Precision Coating Vapor Processing Opportunity Drives Development and Utilization Doing Things Only 3M Can Do Markets Architecture & Construction Consumer & Office Display Electronics Filtration Health Care Renewable Energy Safety & Security Telecoms and Utilities Water Infrastructure M. All Rights Reserved.

6 A Century of Innovation M patented and introduced Wetordry TM waterproof sandpaper the world s first Scotchlok water-resistant coated electrical 1960 abrasive Scotch TM masking tape introduced 1927 Scotch TM Cellulose tape introduced M began producing Colorquartz roofing granules 1939 First traffic sign featuring Scotchlite reflective sheeting erected in Minneapolis connectors and Scotchkote insulation introduced 3M debuted its first surgical drape Scotch TM magnetic audiotape introduced 3M s first non-woven productdecorative ribbon for gifts introduced 1954 RCA used Scotch TM magnetic tape to record TV programs for the first time Scotch TM Brand Magic transparent tape introduced M products were used in the first moon walk on July 20. Astronaut Neil Armstrong left a footprint in the lunar dust in boots made from Fluorel synthetic rubber from 3M 3M introduced the first metered dose asthma inhaler, free of ozone depleting chlorofluorocarbons M introduced Scotchshield window film, shatter-resistant, heatand cold resistant window protection Academy of Motion Picture Arts and Sciences gave 3M a Scientific Engineering Award for magnetic film, improving audio capabilities of movie sound tracks M LED advanced Scotch TM vinyl 3M introduced light, most electrical Thinsulate thermal Post-it notes, energy efficient, 6 tape introduced insulation introduced M. All Rights Reserved. greatly enhancing office M developed the first disposable facemasks and respiratory protection products 1996 New products include flexible circuits for electronic products and HFEs (hydrofluoroethers), replacing ozone depleting chlorofluorocarbons M introduced the Vikuiti brand for light management products that make electronic displays easier to read M developed Aluminum Conductor Composite Reinforced (ACCR) as a solution to thermally constrained transmission bottlenecks M TM MPro pocket-sized projector for mobile presentations and sharing photos on the go M TM Cubitron TM II using precision shaped abrasive granules creating the future of the abrasives industry longest-lasting light bulb

7 Yeast & Mold Testing Yeasts & Molds are an important quality indicator in the food and beverage industry, impacting spoilage and shelf life one of the top four indicator organisms Estimated to be 14% of total indicator testing volume Important food segments for Yeast & Mold: Cultured dairy, fruits & vegetables, bakery products, confectionary, RTE meats, nuts, spices, dry goods Characteristics of Yeast & Molds: Staph aureus 6% Indicator Tests Worldwide EB 6% Yeast and Mold 14% E. coli 15% Other 10% Total Ct. 34% Coliform 15% Grows well in low ph and low water activity Requires long incubation time: 5 days (reference method) vs. most indicator organisms are incubated hrs. Difficult to interpret (count): Molds grow as a irregular spreading mass (as opposed to a pinpoint colony like bacteria) M. All Rights Reserved.

8 Reference methods for Yeast & Mold testing FDA-BAM Reference Methods FDA-BAM, Chapter 18 Spread-plate or Pour-plate DRBC, DG18 or PDA, 25 o C, 5-7 days* 0.1% Peptone water g sample, 11 ml/99 ml (Dairy) Dairy (SMEDP) Spread-plate Yeast extract glucose chloramphenicol agar, PDA or DRBC, 25 o C, 5 days* Butterfield s phosphate buffered water ISO Reference Methods ISO (A w >0.95) Spread-plate Dichloran rose bengal chloramphenicol agar (DRBC) 0.1% Peptone water, 25 o C, 5 days ISO (A w <0.95) Spread-plate Dichloran 18% glycerol agar (DG18) 0.1% Peptone water, 25 o C, 5-7 days ISO 6611: 2004 (Dairy) Pour-plate Yeast extract glucose chloramphenicol agar 25 o C, 5 days * Count plates after 5 days of incubation. If there is no growth at 5 days, re-incubate for another 48 h M. All Rights Reserved.

9 Comparison of Techniques Spread Plate Process Pour Plate Process 3M Petrifilm Plate Process Prepare Agar Pour plates, allow to solidify Prepare Agar Prepare Sample Prepare Sample Prepare Sample 1 st Dilution: Dispense 1mL over 3 plates Spread inoculum on each plate 0.3mL 0.3mL 0.4mL 1 st Dilution: Inoculate empty dish Pour agar, swirl, allow to solidify 1.0 ml 1 st Dilution: Inoculate 3M Petrifilm Plate 2 nd Dilution: Dispense 0.1mL of first dilution on one further plate Spread inoculum 0.1mL 2 st Dilution: Make further 1:10 dilution, inoculate 1mL, pour agar, allow to solidify 1.0 ml 2 st Dilution: Make further 1:10 dilution, inoculate 1mL, on 3M Petrifilm Plate 3 rd Dilution: Make 1:10 dilution of the first dilution Dispense and spread 0.1mL over one plate 0.1mL 3 rd Dilution: Make further 1:10 dilution, inoculate 1mL, pour agar, allow to solidify 1.0 ml 3 rd Dilution: Make further 1:10 dilution, inoculate 1mL, pour agar 25 Incubate Incubate Incubate M. All Rights Reserved

10 Why does Yeast & Mould testing take so long? Yeast & Mould s usually take much longer to grow and be counted than other microbiology tests The group are very different from other microbes we count in traditional microbiology testing Eukaryotic cells as compared to bacteria that are Prokaryotic Very different from Bacteria including size Often have slower metabolic pathways Utilise different growing conditions Aerobic Can grow in lower Water Activity than bacteria Lower ph Often produce antibiotics so can be difficult competitors M. All Rights Reserved.

11 Slow growing due to complex physiology M. All Rights Reserved.

12 Complex cellular organisation M. All Rights Reserved.

13 Times of some common Food microbiology tests Total Viable Count 48 hrs with most food matrices Some newer tests allow this result within 24 hrs E.coli Testing 24 hrs Enterobactericeae testing 24 hrs with VRBGA or Petrifilm Yeast & Mould Testing 5 days M. All Rights Reserved.

14 Food testing for Yeast & Mould contamination Food testing for Yeast & Mould contamination is normally relate to food spoilage and not food poisoning Only a few relevant mycology food poisoning cases are seen every year Commercially it has a huge impact on the value of food and the amount of food wasted globally Mould contamination can be a very visual issue Yeast contamination often causes taste changes in a food sample M. All Rights Reserved.

15 Difficult to read As Yeast & Moulds are a very large group we often get mixed colonies on a plate These can overlap and make reading very difficult In some cases a 3 day and a 5 day read are needed M. All Rights Reserved.

16 Laboratory Contamination Opening an agar plate can lead to contamination of your lab with mould spores For weeks after none selective media can be contaminated with small mould colonies This can result in the need for cleaning with harsh chemicals such as Phenols In severe cases the lab can need to be fumigated Adds a lot of inconvenience and expense in the lab M. All Rights Reserved.

17 Rapid Petrifilm Yeast & Mold Plate Product Summary Description: 3M Petrifilm Yeast & Mold plate with significantly improved time-to-result and interpretation. Key Features & Benefits: Yeast & Mold Plate with hour incubation time across a wide variety of foods. AOAC RI and ISO-AFNOR validated results Easier interpretation on Yeast & Mold plate (special technology that keeps mold colonies from spreading out and overlapping) New indicator dye technology colors colonies blue and make them appear faster. Foam dam added to plate for easier inoculation All of the traditional benefits of Petrifilm plates are maintained: simplicity, ease-of-use, shelf life, consistency, eliminates agar prep. Image of plate post-incubation: colonies indicated by blue color M. All Rights Reserved.

18 Comparison between traditional agar & Rapid Y&M plates RY&M Petrifilm Advantages Shorter incubation times up to 50% faster Easier interpretation less time to read the plates Single media use High and low water activity on single plates Ready to use. No wait to use the product Consistent (Especially important with the addition of antibiotic supplements. When this is missed out the results given are not accurate) Long shelf life 18 months compared to 3-4 weeks Smaller footprint 90% space saving Less waste 90% less material to autoclave / pay to dispose Sealed plates Reduce the risk of laboratory contamination M. All Rights Reserved.

19 Thanks for your attention Louise Mare Technical Manager 3M South Africa M. All Rights Reserved.