Thermo Scientific HyClone Research Cell Culture Products. Quality Consistency Reliability

Transcription

1 Research Cell Culture Products Quality Consistency Reliability

2 Company Overview Thermo Scientific HyClone A History of Innovation and Quality Our mission is to be the leading supplier of quality solutions for challenges faced in the biopharmaceutical industry and cell culture-based research. Our commitment to provide the most innovative tools to facilitate and advanced research is the foundation for all Thermo Scientific Cell Culture and BioProcessing Products. For more than 40 years, Thermo Scientific Cell Culture and BioProcessing Product experts have remained dedicated to the advancement of science through the support of cell culture and a comprehensive commitment to academia, research and bioprocessing. As a global manufacturer of sera, liquid and powdered media, process liquids and handling systems to support research and production of biopharmaceuticals, Thermo Scientific HyClone products continue to be produced to the highest and most consistent quality standards. Our experts understand the products needed to facilitate cell culture-based research. As a leading supplier of sera, media and other cell culture reagents, we strive to provide innovations and advances in cell culture that continually expand our knowledge and expertise. 2 Visit our web site at Closed-system cardiacpuncture collection

3 Sera Consistent Products Guaranteed For decades, our sera products have set the industry standard for quality, purity and regulatory compliance. Today our sera line offers you more features than ever. Our serum collection process is vertically integrated like no other in the industry. Great care in collection and processing ensures the serum you use to produce your end product is the best and most biologically active. Our portfolio contains a range of products designed to be specific for biotechnology applications. We provide exclusive processes to enhance the level of consistency, quality and safety of our product to meet specific needs. With our active role in emerging markets including new human vaccines, we are positioned to provide consistent, high performance cell culture products to enable advances in life sciences research. Whether you require fetal bovine serum, serum alternatives, process supplements or other animal origin sera products, we are positioned to provide you the very best solutions. The Benefits Degree of vertical integration Expert technical assistance Variety of specialty fetal bovine sera Technical application notes State-of-the-art filtration options Lot-to-lot consistency Sera supply history Origin stated and certified Validated post-filtration processes Traceability Our sera operations are located strategically in New Zealand, Australia, the United States, Canada and Central America to ensure safety and traceability. By using large batch sizes and true pool technique, we are able to produce lot-to-lot consistency, and lot-specific data to ensure the serum you order today, closely matches the biochemical profile of the batch you purchased previously. Thermo Scientific HyClone If you are not 100% satisfied with the quality or performance of our sera products, notify us within 30 days and we will replace any unopened product at no charge. * *Subject to terms and conditions at or contact customer service at To order or request additional information, call Traceability 3

4 Global Markets, Origin and Quality Thermo Scientific HyClone Animal Sera Origins Serum origin refers to the country in which the raw blood was collected. This is not to be confused with the country in which the raw serum was sterile filtered, having originated elsewhere. With HyClone Sera products, the country of origin is not a secret. The documentation you receive will clearly state the country or countries of origin. This information is on the Certificate of Analysis and Certificate of Origin. New Zealand The great expanse of the Pacific Ocean has protected New Zealand s islands from many outside influences, both geographically and biologically. One of the benefits of this isolation is that New Zealand has the fewest reported bovine diseases in the world and the USDA has determined it to be free from BSE and FMD. This makes New Zealand FBS an excellent source of bovine serum as it offers maximum safety. New Zealand FBS is carefully collected, processed and filtered in our own New Zealand facility. Australian Our Characterized Australian FBS is sourced from only USDA or Australian Quarantine and Inspection Service approved abattoirs in Australia. We have found that Australian methods of animal husbandry are world class with unsurpassed animal nutrition and healthcare. As with New Zealand, Australia is an island continent thus making animal disease control and management easier than in most areas of the world. The USDA also considers Australia to be free of BSE and FMD. Innovative Techniques to Meet Demands There are specific requirements for serum used in a variety of cell culture applications involved with modern biopharmaceutical research; therefore, a number of processing methods are employed to yield a suitable product for specific applications. Some treatments are used to remove adventitious agents and other processes filter various natural serum constituents that may disrupt certain analytical techniques or assays. Based on our serum processing and application understanding, we have the ability to perform a number of tests to identify serum lots with characteristics that make them ideal for specific cell lines and protocols. Post-Filtration Treatments Gamma Irradiation: This is a powerful tool to further reduce the risk of adventitious viruses present in serum. The irradiation process used should be validated to deliver a minimum dose of 25 kgy. If manufacturing products for use in humans or in animals, irradiation should be a routine requirement. Heat Inactivation: Exposing serum to a temperature of 56 C for 30 minutes is a common post-filtration process. It may be effective in reducing some adventitious agents and may be used in addition to, but never substituted for, gamma irradiation. Both services are available by request. USDA USDA Tested FBS is sourced from several Central American countries (Costa Rica, Honduras, Panama, Nicaragua, El Salvador, and Guatemala) which are all recognized as being free of BSE and FMD by the USDA. Serum from Central America is USDA safety tested. 4 Visit our web site at Extensive quality control

5 Testing Sera Testing Results Description and Origin Part Number Filtration Test Endotoxin (EU/mL) Hemoglobin (mg/dl) Total Protein (gm/dl) Osmolality mosm/kg) Cytopathogenic agents Sterility Bacteria and Fungi testing Fluorescent antibody testing Mycoplasma Hemadsorbing agents Protein testing Trace metals / Iron Vitamins / Hormones Fetal Bovine Serum Defined FBS, US SH nm Characterized FBS, US SH30071 Triple 100 nm Standard FBS, US SH30088 Triple 100 nm FIO FIO Characterized FBS, NZ SH30406 Triple 100 nm Characterized FBS, Australian SH30084 Triple 100 nm Characterized FBS, Canadian SH30396 Triple 100 nm USDA Tested FBS, Central American SH30910 Triple 100 nm Specialty Fetal Bovine Serum Super Low IgG FBS, US SH nm Charcoal/Dextran Treated FBS, US SH30068 Triple 100 nm Dialyzed FBS, US SH30079 Triple 100 nm ES Cell Sreened FBS, US SH30070E 40 nm Tetracycline Screened FBS, US SH30070T 40 nm Insect Cell Screened FBS, US SH30070I 40 nm Human Mesenchymal SC Screened FBS SH30070M 40 nm Bovine Calf Serum Bovine Calf Serum, US SH30073 Triple 100 nm Iron Supplemented Bovine Calf Serum, US SH30072 Triple 100 nm Cosmic Calf Serum, US SH30087 Triple 100 nm Bovine Growth Serum, US SH30541 Triple 100 nm Newborn Bovine Calf Serum, US SH30118 Triple 100 nm Bovine Calf Serum, NZ SH30403 Triple 100 nm Cosmic Calf Serum, NZ SH30413 Triple 100 nm Bovine Growth Serum, NZ SH30591 Triple 100 nm Donor Bovine Calf Serum, NZ SH30417 Triple 100 nm Fetal Bovine Serum Alternatives FetalClone I Serum, US SH30080 Triple 100 nm FetalClone II Serum, US SH30066 Triple 100 nm FetalClone III Serum, US SH30109 Triple 100 nm Alpha Calf Serum, Iron SH30076 Triple 100 nm Supplemented, US Alpha Calf Serum Without Iron, US SH30212 Triple 100 nm Other Serum Donor Equine Serum, US SH30074 Triple 100 nm Donor Adult Bovine Serum, US SH30075 Triple 100 nm Adult Bovine Serum, NZ SH nm Porcine Serum, NZ, IR SH nm Electrophoretic profile IgG (μg/ml) Thermo Scientific HyClone = Tested. For typical results request Certificate of Analysis or go to To order or request additional information, call Validated mycoplasma removal 5

6 Fetal Bovine Serum Thermo Scientific HyClone Fetal Bovine Serum Fetal Bovine Serum As FBS is our flagship product line, we offer an unparalleled and extensive line of specialty serum for research applications. Defined Defined FBS is widely used by cell culturists who have a concern for viral contaminants and require an extensive biochemical profile. Defined FBS is filtered through serial 40 nm (0.04 µm) pore-size rated filters. Highest-quality FBS on the market 40 nm filtered Lowest endotoxin and hemoglobin specifications Characterized Comparable to the highest quality FBS from other suppliers, Characterized FBS is lower in price than Defined FBS and meets the requirements of most discriminating FBS users. Standard endotoxin and hemoglobin specifications Standard Our standard quality cell culture grade FBS is used by price-conscious cell culturists. It is procured in the same manner as Defined and Characterized FBS. Excellent choice for cost-sensitive applications Endotoxin and hemoglobin are tested and reported Defined Fetal Bovine Serum Product Part Number Volume Defined FBS CofS* R1-CEP ; 40 nm filtered, Charaterized Fetal Bovine Serum SH SH SH ml Product Part Number Volume Characterized FBS CofS R1-CEP ; 3x100 nm filtered, 25 EU/mL Endo, 25 mg/dl Hemo; USDA Tested FBS CofS RO-CEP ; 3x100 nm filtered, 25 EU/mL Endo, 25 mg/dl Hemo; can be BVD+, Hon, Pan, CR, El Sal, Guat, Nic Canadian Characterized FBS CofS R0-CEP ; 3x100 nm filtered, 25 EU/mL Endo, 25 mg/dl Hemo; can be BVD+ NZ Characterized FBS CofS R1-CEP ; 3x100 nm filtered, 20 EU/mL Endo, 25 mg/dl Hemo Australian Characterized FBS CofS R1-CEP ; 3x100 nm filtered, 25 EU/mL Endo, 25 mg/dl Hemo SH SH SH SH SH SH SH SH SH SH SH ml Standard Fetal Bovine Serum Product Part Number Volume Standard FBS CofS R1-CEP ; 3x100 nm filtered, Endo & Hemo tested/reported; can be BVD+ SH SH *Certificate of Suitability available at 6 Visit our web site at 40 nm pore-size filtration

7 Specialty FBS Products In order to better serve researchers, we have developed an extensive line of Specialty FBS products. These products are designed for specific applications and are produced using Defined FBS. Super Low IgG FBS This product is designed for applications where extremely low levels of inherent bovine IgG are required. Our Super Low IgG FBS has been chromatographically treated to reduce IgG levels to less than 5 μg/ml while still retaining superior cell growth properties. For use in monoclonal antibodies production, virus propagation and immunoassay procedures Charcoal/Dextran Treated FBS Our exclusive proprietary treatment has been shown to reduce steroid levels and can be utilized in receptor studies or estrogen related investigations. A unique feature of this product is the extensive biochemical profile of the serum provided both before and after the treatment. This assures efficacy of the treatment and provides end-users with critical information regarding which components are specifically affected. Proprietary processing used to reduced levels of various hormones and various growth factors Lipid-Reduced FBS Our Lipid-Reduced FBS is a ready-to-use specialty product designed for researchers requiring lower levels of lipids. A Certificate of Analysis provides an extensive profile of various lipids and related components. Processed to reduce lipids and related components Specialty Fetal Bovine Serum Specialty Fetal Bovine Serum Product Part Number Volume Super Low IgG FBS CofS R1-CEP ; 40 nm filtered, IgG Spec: 5 μg/ml; Charcoal/Dextran FBS CofS R1-CEP ; 3x100 nm filtered, 10 EU/mL Endo, 20 mg/dl Hemo; Lipid Reduced FBS CofS R1-CEP ; 3x100 nm filtered, Dialyzed FBS CofS R1-CEP ; 3x100 nm filtered, 10,000 MW cutoff; SH SH SH SH SH SH SH SH SH SH ml 50 ml Thermo Scientific HyClone Specialty Fetal Bovine Serum Dialyzed Dialysis reduces concentrations of low molecular weight components such as nucleotides and amino acids necessary for alternative biochemical survival pathways. The process is reproducible and reduces hypoxanthine and thymidine concentrations below detectable limits making Dialyzed FBS ideal for incorporation or receptor studies. Processed using proprietary diafiltration method Depletes serum of small molecules (less than 10,000 MW) To order or request additional information, call Triple 100 nm filtration 7

8 Screened Fetal Bovine Serum Thermo Scientific HyClone Screened FBS As part of our Specialty FBS Product line, we screen our Defined FBS for specific applications. These prescreened products save researchers valuable time and money. Murine Embryonic Stem Cell ES Screened FBS supports the growth of undifferentiated cells. The screening includes plating efficiency, colony morphology and toxicity tests. Optimal for the culture of murine embryonic stem cells Human Mesenchymal Stem Cell Essential to ensure optimum performance when culturing undifferentiated human mesenchymal stem cells (hmsc), this FBS is proven to support superior growth. During screening, hmsc are observed for evidence of nutritional deficiency, cytotoxicity or morphological aberrations. Designed for human mesenchymal stem cells Tetracycline Designed for researchers using tetracycline-regulated gene expression systems in cultured cells, Tetracycline Screened FBS is ideal for expression studies in Tet-on/Tet-off systems. FBS with undetectable levels of tetracycline Screened Fetal Bovine Serum Screened Fetal Bovine Serum Product Part No. Volume ES Screened FBS CofS R1-CEP ; 40 nm filtered, Human Mesenchymal Stem Cell Screened FBS 40 nm filtered, 10 EU/mL Endo, 10 mg/dl Hemo; Tetracycline Screened FBS CofS R1-CEP ; 40 nm filtered, Insect Cell Screened FBS CofS R1-CEP ; 40 nm filtered, SH E SH E SH M SH T SH I Insect Cell With the increasing popularity of the Baculovirus Expression Vector Systems (BEVS), more researchers are culturing insect cells. Our Insect Cell FBS is screened to support robust growth of insect cells. For optimal performance with insect cells 8 Visit our web site at Database of over 200 biochemical assays

9 Bovine Calf Serum Bovine Calf Serum Our Bovine Calf Sera products are an excellent and cost-effective alternative to FBS. Our Bovine Calf Serum contains exceptionally high levels of transferrin, and when supplemented, it can provide three to four times as much available iron as FBS. In many applications, calf serum will provide equivalent or even surpass performance of FBS. Iron Supplemented This Iron-Supplemented Bovine Calf Serum contains three to four times as much available iron and transferrin as FBS or equine serum. Excellent, cost-effective alternative to FBS Cosmic High-quality iron-supplemented bovine calf serum augmented with additional growth promoting components yield a superior bovine calf serum fortified with iron and naturally derived components. Increased performance with a variety of cell types Bovine Growth Serum Defined bovine calf serum supplemented with chemically-defined components such as vitamins, amino acids, trace metals and other small molecules. Ideal for stimulating cell growth and proliferation Newborn The same careful collection and processing procedures, including venipuncture, are used for our newborn calf serum, as well as all our bovine serum products. High-quality FBS alternative Bovine Calf Serum Product Part No. Volume Bovine Calf CofS R1-CEP ; 3x100 nm filtered, Age at time of collection: weeks Iron Supplemented Bovine Calf CofS R1-CEP ; 3x100 nm filtered, Age at time of collection: weeks Cosmic Calf CofS R1-CEP ; 3x100 nm filtered, Age at time of collection: weeks Bovine Growth Serum (BGS) CofS R1-CEP ; 3x100 nm filtered, Age at time of collection: weeks Newborn Calf Serum 3x100 nm filtered, 50 EU/mL Endo, 25 mg/dl Hemo; Age at time of collection: <10 days NZ Newborn Bovine Calf Serum CofS R1-CEP ; 3x100 nm filtered, 25 EU/mL Endo, 25 mg/dl Hemo; Age at time collection: average 24 months NZ Cosmic Calf Serum CofS R1-CEP ; 3x100 nm filtered, 25 EU/mL Endo, 25 mg/dl Hemo; Age at time collection: <10 days SH SH SH SH SH SH SH SH SH SH SH SH SH SH SH SH SH SH SH ml Thermo Scientific HyClone Bovine Calf Serum New Zealand Calf Serum With our New Zealand FBS products, raw material is carefully collected, processed and filtered to offer maximum safety against bovine diseases. We follow the same standards in processing our New Zealand calf serum as with our FBS to ensure quality and absolute traceability. Secure filtration and processes for production To order or request additional information, call Lot-to-lot consistency 9

10 FBS Alternatives FBS Alternatives Thermo Scientific HyClone FBS Alternatives Our FBS alternatives are designed to be a high-performance replacement readily available at a reduced cost. Alpha Calf This product is produced from our bovine calf serum using a proprietary non-ethanol process to reduce immunoglobulins and proteins to levels very similar to those of FBS. Alpha Calf Fraction typically contains less than 200 μg/ml IgG. It is available with and without iron. Optimized for the growth of hybridomas Cost-effective medium supplement FetalClone I Optimized for the growth of hybridomas IgG levels comparable to those found in FBS FetalClone II Optimized for the growth of CHO cells and derivatives Offering the same basic formulation as our FetalClone I, plus additional growth factors and supplements No adaptation required Suggested concentrations similar to those used with FBS FetalClone III The most widely applicable of our FetalClone family Designed to include fibroblasts in its applications FBS Alternatives Product Part No. Volume Alpha Calf Fraction 3x100 nm filtered, 10 EU/mL Endo, 20 mg/dl Hemo; Age at time collection: weeks Iron Supplemented Alpha Calf Fraction 3x100 nm filtered, 10 EU/mL Endo, 20 mg/dl Hemo; Age at time collection: weeks FetalClone I Serum CofS R1-CEP ; 3x100 nm filtered, 10 EU/mL Endo, 20 mg/dlhemo; Growth of hybridomas; FetalClone II Serum CofS R1-CEP ; 3x100 nm filtered, 10 EU/mL Endo, 20 mg/dlhemo; Growth of CHO-K1 and CHO derivatives; FetalClone III Serum CofS R1-CEP ; 3x100 nm filtered, 10 EU/mL Endo, 20 mg/dlhemo; Most widely applicable, including fibroblasts; SH SH SH SH SH SH SH SH SH Visit our web site at liter lot sizes

11 Species Specific Sera Donor Adult Bovine Our Donor Adult Bovine serum is sourced from a closed herd maintained on USDA-licensed premises. Cattle from these closed herds are used exclusively for the production of donor bovine blood products. Donor Equine Equine serum is carefully collected from animals fed an enriched diet to ensure proper nutrition. The horses are bled in an enclosed facility specially equipped for aseptic collection after being fasted to reduce lipid concentration. Coggins tests for equine infectious anemia are routinely performed on each horse. New Zealand Donor Sourced from New Zealand, this serum is processed and sterile filtered using three sequential 100 nm (0.1 Rm) pore-size rated filters. New Zealand Porcine Our Porcine serum is sourced from New Zealand and filtered in the US through multiple 200 nm (0.2 µm) pore-size rated filters and is gamma irradiated at kgy after filtration. Species Specific Sera Products Product Part No. Volume Donor Adult Bovine Serum CofS R1-CEP ; 3x100 nm filtered, 50 EU/mL Endo, 25 mg/dlhemo; Age at time of collection: <36 months Donor Equine Serum (CofS N/A); 3x100 nm filtered, 10 EU/mL Endo, 10 mg/dlhemo; Age at time of collection: >6 months NZ Adult Bovine Serum CofS R1-CEP ; 3x200 nm filtered, 100 EU/mL Endo, 50 mg/dlhemo; Age at time of collection: average 6-8 years NZ Donor Bovine Serum CofS RO-CEP ; 3x100 nm filtered, 10 EU/mL Endo, 10 Hemo tested/reported; Age at time collection: <24 months NZ Iron Supp Newborn Serum CofS R1-CEP ; 3x100 nm filtered, 25 EU/mL Endo, 25 mg/dlhemo; Age at time collection: <10 days NZ Porcine Serum (CofS N/A) filtered in the U.S., irradiated 3x200 nm filtered, 100 EU/mL Endo, 50 mg/dl Hemo, Age at time collection: >6 months SH SH SH SH SH SH SH SH SH SH SH SH Thermo Scientific HyClone Species Specific Sera True pool processing To order or request additional information, call

12 Sera Products We Provide High Quality FBS We Care For Our Customers Thermo Scientific HyClone Sera Products Serum Collection From collection to final packaging, every step is documented to guarantee a consistent high-quality product with the lowest possible risk of contamination and full traceability compliant with current Good Manufacturing Practices (cgmp). Quick and Reliable Processing Finished products undergo extensive quality, performance and analytical testing. Goods are aseptically packaged in a Class 100 cleanroom into gamma irradiated plastic bottles. Quality Control Testing and Standards Each lot of sera is tested to ensure quality and sterility. Low endotoxin and haemoglobin levels reflect the care with which the serum is collected and processed. Batch Testing and Reserve Without any obligation or cost, we will send serum sample or samples for testing in your specific application. During the testing period, we will reserve one or more batches. Stock Management We can help you plan the most effective way to order and stock your serum. Your serum representative can readily provide an overview of deliveries and adjust current reserve if required. 12 Visit our web site at Component testing

13 Stem Cells AdvanceStem Cell Culture Platform Somatic Stem Cell Culture Products These products have been developed specifically for applications in stem cell research. The line comprises products designed to support the expansion and maintenance of undifferentiated human mesenchymal stem cells, as well as the directed differentiation of these cells into such types as adipocytes, chondrocytes, osteocytes and neurons. Murine Embryonic Stem Cell Culture Products Providing optimal culture conditions can be one of the greatest challenges in stem cell research, particularly in keeping cells in an undifferentiated state or directing differentiation when desired. AdvanceSTEM Murine Embryonic Stem Cell products can help you meet those challenges. Extracellular Matrix HyStem Hydrogels HyStem Hydrogels provide a wide array of extracellular matrices designed specifically to meet the exacting requirements of the stem cell researcher. HyStem Hydrogels are biocompatible matrices which can be easily customized to provide the ideal surface for a specific stem cell type. Media Product Part No. Available Volumes AdvanceSTEM Low OsmoDMEM w/o L-Glutamine Stem Cells Product Part No. Available Volumes Human Wharton s Jelly MSCs SV ,000 cells, 500,000 cells Human Adipose-Derived MSCs SV ,000 cells, 500,000 cells Human Amniotic MSCs SV ,000 cells, 500,000 cells Human Amniotic Epithelial SCs SV ,000 cells, 500,000 cells Multipotent Cord Blood Unrestricted Somatic SCs SV ,000 cells, 500,000 cells Human Bone Marrow MSCs SV ,000 cells, 500,000 cells Human Cord Blood Hematopoietic CD34+ SCs Human Cord Blood Hematopoietic CD133+ SCs SH30870 SV30106 SV30107, AdvanceSTEM Serum Replacement SH30874, AdvanceSTEM Mesenchymal Stem SH30875.KT Kit Cell Expansion Kit Amniotic Epithelial Expansion Kit SH30904.KT Kit AdvanceSTEM Adipogenic SH30876.KT Kit Differentiation Kit AdvanceSTEM Osteogenic SH30877.KT Kit Differentiation Kit AdvanceSTEM Neural Differentiation Kit SH30892.KT Kit 100,000 cells, 500,000 cells 100,000 cells, 500,000 cells PRX-B6N Murine ESCs SV x 10 6 cells PRX-129/X1 Murine ESCs SV x 10 6 cells Thermo Scientific HyClone AdvanceSTEM Products Extracellular Matix Product Part No. Available Volumes HyStem Kit 2.5 ml SV ml, 7.5 ml HyStem-C Kit 7.5 ml SV ml, 7.5 ml HyStem-HP Kit 2.5 ml SV ml, 7.5 ml To order or request additional information, call Superior stem cells and reagents 13

14 Media Thermo Scientific HyClone Media Products Media Classical Liquid Media Our extensive line of Classical Media products are system-tested with Thermo Scientific HyClone sera to ensure product efficacy and homogeneity. These products are available in convenient packaging for research applications. Commonly used products are shown in the product list to the right. For a complete list of products, please visit our web site. Reagents and Cell Dissociation Products HyQTase Thermo Scientific HyQTase is an ultra-filtered, non-mammalian formulation that provides a safe, gentle Trypsin / EDTA alternative. Diminished cell damage Improved cell yield Enhanced cell viability Trypsin Our product offering includes two commonly used concentrations of gamma-irradiated porcine trypsin (1:250 and 1:50). Antibiotics and Selection Agents We offer a selection of premium antibiotics to control bacterial, yeast, fungi and mycoplasma contaminations. Cell Culture Grade Water (Endotoxin-free) Thermo Scientific HyClone Cell Culture Grade Water is not just endotoxin screened, it is endotoxin-free. The assurance that water is free of endotoxin not only quells concerns about the reagent s properties, but it also assures that the product was prepared with the best of processes and procedures. In addition to this, we offer WFI Quality Water, Molecular Biology Grade Water and a variety of other process liquids. For a complete list of products, please visit our web site. Media Product Part No. Available Volumes RPMI 1640 Medium with 25 mm HEPES, with L-Glutamine DMEM with High Glucose, with 4.0 mm L-Glutamine, with Sodium Pyruvate DMEM with High Glucose, with 4.0 mm L-Glutamine, without Sodium Pyruvate DMEM with High Glucose, without L-Glutamine and Sodium Pyruvate SH30255 SH30243 SH30022 SH30081,, 6 x,, 6 x, 6 x,, 6 x, 6 x,, 6 x, 6 x Grace s Unsupplemented SH30610 IMDM with L-Glutamine, HEPES, without Alpha-Thioglycerol SH30228,, 6 x MEM with Earle s Balanced Salt Solution (EBSS), with 2.0 mm L-Glutamine HyQTase Cell Detachment Solution Trypsin 0.25% Trypan Blue Solution 0.4% in Phosphate Buffered Saline Gentamicin Solution 50 mg/ml Antibiotic Antimycotic Solution (Pen/Strep/Fungizone), 100X SH30024 SV30030 SV30031 SV30084 SV30080 SV30079,, 6 x, 6 x 10 x 10 ml, Phosphate Buffered Saline (PBS) SH30256,, 6 x, 6 x Dulbecco s Phosphate Buffered Saline (DPBS), HEPES 1M Solution Earle s Balanced Salt Solution (EBSS) 1X, with Calcium, Magnesium, Phenol Red Hank s Balanced Salt Solution (HBSS) 1X, with Calcium, Magnesium, Phenol Red SH30264 SH30237 SH30029 SH30030,, 6 x,, L-Glutamine SH30034, NEAA SH30238 Cell Culture Grade Water Endotoxin-free (< EU/mL), Deionized, Distilled, 0.1 µm Sterile Filtered SH30529,,, 6 x, 6 x 14 Visit our web site at System tested with HyClone Serum

15 Media Serum-Free Media Applications for HyClone Serum-Free Media (SFM) can be found in many biotechnology fields, including the production of human and animal biopharmaceuticals, diagnostic reagents and bioagricultural products. Due to increased attention on producing biologicals representative of their native form, our Serum-Free Media focuses on supporting mammalian and invertebrate cell culture platforms. Some of the critical cell culture applications of these platforms include the expression of recombinant proteins, monoclonal antibodies, viral vectors for gene therapy, and viral vaccines. These applications constitute the majority of biopharmaceutical research, development and manufacturing employing eukaryotic cell culture. Developed through the Metabolic Pathway Design approach, our Serum-Free Media support superior performance in multiple cell culture platforms. The objective for developing serum-free media through Metabolic Pathway Design is to provide the intended cell line with the nutrients needed to promote cell growth and stimulate productivity. This development process produces SFM targeted to increase process yields for each respective cell platform. Current HyClone SFM applications include hybridoma, NS0, insect, Chinese Hamster Ovary (CHO), PER.C6 *, HEK 293, MDCK, MDBK, COS-7 and Vero cell cultures. The use of serumfree media provides many time- and cost-saving advantages, including: Eliminating the need to pre-screen serum lots Simplified regulatory documentation Consistent media performance Reduced downstream purification challenges Serum-Free Media CHO Cell Culture Part No. Available Volumes CDM4CHO with 4.0 mm L-Glutamine SH30557, CDM4CHO SH30558, SFM4CHO with 4.0 mm L-Glutamine SH30549, SFM4CHO SH30548, SFM4CHO-Utility with 4.0 mm L-Glutamine Hybridoma and Myeloma Cell Culture CDM4MAb with 6.0 mm L-Glutamine SH30516 SH30801,, CDM4MAb SH30802, CDM4NS0 SH30579, SFM4MAb with 6.0 mm L-Glutamine SH30513, SFM4MAb SH30391, SFM4MAb-Utility with 6.0 mm L-Glutamine ADCF-MAb with 4.5 mm L-Glutamine SH30382 SH30349,, ADCF-Mab SH30547, HEK293 and PER.C6 Cell Culture SFM4Transfx-293 SH30860, CDM4HEK293 SH30858, CDM4PERMAb SH30871, SFM4HEK293 with 4.0 mm L-Glutamine CDM4Retino with 4.0 mm L-Glutamine Insect Cell Culture SFM4Insect with 10 mm L-Glutamine SFX-Insect with 10 mm L-Glutamine Viral Vaccine Cell Culture SH30521 SH30520 SH30913 SH30278,,,, Thermo Scientific HyClone Media Products SFM4MegaVir SH30552, *PER.C6 is a registered trademark of Crucell NV. To order or request additional information, call ADCF, chemicallydefined formulations 15

16 2010 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. SM0513 Cell Culture & BioProcessing 925 West 1800 South Logan, UT In Americas/Asia fax In Europe fax