Quick-DNA. DNA from any Sample

Transcription

1 Quick-DNA DNA from any Sample

2 Quick-DNA Overview Zymo Research offers a range of genomic DNA isolation kits that are suitable for extracting high molecular weight DNA from a wide variety of sample types including tissue, fresh and paraffinembedded tissue sections, cultured cells, saliva, buccal cells, whole blood, plasma, serum, urine, bacteria, fungi, yeast, algae, viruses, and mitochondria. Our genomic DNA isolation kits yield high-quality DNA that is ideal for use in any sensitive downstream applications such as PCR, DNA sequencing, endonuclease digestion, and methylation detection. Biological Fluids, Cells & Tissues Quick-DNA Plus Kits Liquid Biopsy Serum, Plasma, Urine, etc. Quick-cfDNA Serum & Plasma Kit Quick-DNA Urine Kit FFPE Samples Quick-DNA FFPE Kit Microbial Plants Cultures Environmental (Fecal, soil, water filtrate and other) Quick-DNA Plant/Seed Kits Quick-DNA Fungal/ Bacterial DNA Kits Quick-DNA Fecal/Soil Microbe DNA Kits For DNA isolation in microbiomic studies, see our ZymoBIOMICS product line. Learn more at /dna/quick-dna

3 Quick-DNA Plus Kits Highlights Extract high-quality DNA easily and reliably from any biological fluids, cultured/monolayer cells, or solid tissues Zymo-Spin technology ensures DNA is ready for all sensitive downstream applications such as qpcr, DNA-sequencing, arrays, and methylation analysis. Human Blood Porcine Blood HeLa Cells Buccal Swab Human Saliva Mouse Tail Mouse Kidney Mouse Brain Bovine Muscle Bovine Milk M High-quality DNA from any sample High-quality DNA obtained from a wide range of biological samples using the Quick-DNA Miniprep Plus Kit. DNA purified using the Quick-DNA Miniprep Plus Kit is ultra-pure, highly concentrated, and ready for all downstream applications. Input DNA was standardized to 300 ng and analyzed in a 1% (w/v) TAE/agarose/EtBr gel. The size marker M is a 1 kb ladder (Zymo Research). Purity Redefined Reliable & Consistent Zymo Research 0 μl Carryover vs. Total Yield (µg) R 2 = Supplier Q μl Contamination Zymo-Spin technology ensures no carryover of buffer, salts, or other PCR inhibitors. DNA is ready for all sensitive downstream applications such as qpcr, Next-Generation sequencing arrays, and methylation analysis Human Whole Blood (µl) DNA Yields Increase Linearly with Increasing Volumes of Human Whole Blood Using the Quick-DNA Miniprep Plus Kit. Six replicates of 25, 50, 100, and 200 µl of human whole blood were processed. Product Cat. No. Size Quick-DNA Microprep Plus Kit D4074 D4068T 10 preps. Quick-DNA Miniprep Plus Kit Quick-DNA Miniprep Kit (no Proteinase K) D4068 D4069 D3024 D preps. 200 preps. Learn more and view additional Quick-DNA Kit formats at /dna/quick-dna

4 Quick-cfDNA Serum & Plasma Kit Highlights High-quality DNA, including cell-free, is easily and robustly purified from up to 10 ml of serum/plasma or up to 1 ml amniotic fluid or cerebrospinal fluid. Zymo-Spin Column technology enables elution of DNA in as little as 35 µl and ensures it is ready for all sensitive downstream applications such as qpcr and Next-Generation sequencing. Reliable & Efficient Recovery of Cell-free DNA (up to 10 ml) Plasma/Serum 2 R 2 =0.99 (36yr F serum) R 2 =0.99 (36yr F plasma) [bp] R 2 =0.97 (58yr F serum) cfdna [ng/µl] R 2 =0.97 (58yr F plasma) R 2 =0.99 (54yr F plasma) ml 36 yr F plasma Cell-free DNA recovery is directly scalable using the Quick-cfDNA Serum & Plasma Kit. Graphs and gel image show the linear recovery of cfdna from human plasma and serum (healthy female donors), as measured by 2200 Tapestation (Agilent, in duplicates). Compatible with Many Body Fluids Superior Performance 48,500bp 15,000bp 7,000bp 4,000bp 4,000bp 3,000bp 2,500bp 2,000bp 1,500bp 1,200bp 900bp 600bp 400bp Maternal plasma Amniotic fluid Cerebrospinal fluid Cell free DNA yield (pg/µl) Zymo Supplier Q 250bp 100bp Plasma Volume (ml) Total DNA is efficiently purified from cell-free biological fluids with the Quick-cfDNA Serum & Plasma kit. Total DNA, including both high and low molecular weight species, purified (duplicates) from human maternal plasma, amniotic fluid and cerebrospinal fluid was analyzed by 2200 TapeStation (Agilent). The Quick-cfDNA Serum & Plasma kit robustly recovers cell-free DNA when compared to Supplier Q. The DNA recovered is linearly proportional to the input volume of sample when compared with Supplier Q. Concentration of the smallest nucleosomal fragment (N1, ~180bp) DNA was determined using the 2100 Bioanalyzer (Agilent). Product Cat. No. Size Quick-cfDNA Serum & Plasma Kit D4076 Learn more and view additional Quick-DNA Kit formats at /dna/quick-dna

5 Quick-DNA Urine Kit Highlights Purify cellular and/or cell-free DNA easily and reliably from up to 40 ml of urine. Uniquely formulated urine conditioning reagent allows stabilization of DNA in urine for up to one month at ambient temperature. Zymo-Spin Column technology ensures DNA is ready for all sensitive downstream applications including qpcr, DNA-sequencing, arrays, and DNA methylation analysis. Get cellular or cell-free DNA, or both! Total (Cellular and Cell-free) Cellular only [bp] 48,500 15,000 7,000 Ladder Urine Total DNA Cellular DNA DNA (ng) Cell-free only 4,000 3,000 2,500 2,000 1,500 1, Cell-free DNA Urine (ml) DNA yields increase linearly with increasing volumes of urine from healthy subjects extracted using the Quick-DNA Urine Kit. DNA was isolated from 1 ml, 10 ml, 25 ml, and 40 ml urine. DNA concentration was quantified using the Femto Human DNA Quantification Kit (Zymo Research, E2005). Both cellular and cell-free DNA can be effectively purified from urine using the Quick-DNA Urine Kit. 5 ml of urine from a healthy female donor was processed and DNA was eluted in 20 µl final volume. 1 µl of the sample was analyzed using a 2200 TapeStation. Streamlined, Innovative Workflow Bind Wash Elute Spin DNA Precipitation Proteinase K Digestion DNA Purification Product Cat. No. Size Quick-DNA Urine Kit D3061 Learn more and view additional Quick-DNA Kit formats at /dna/quick-dna

6 Quick-DNA FFPE Kit Highlights Streamlined purification of high-quality FFPE tissue DNA that is ideal for PCR, Next-Generation library prep, enzymatic manipulations, etc. Size selection technology; recover total DNA >50 bp or >500 bp. Streamlined Workflow Selectable DNA Size Deparaffinized Tissue 100bp DNA >500bp 1kb DNA >50 bp $ 500 bp Equivalent amounts of DNA resolved in a 1% agarose/tae/etbr gel show binding conditions may be adjusted with the Quick-DNA FFPE Kit to selectively isolate DNA >50 bp or >500 bp. 100 bp DNA ladder and 1 kb DNA ladder from Zymo Research. Proteinase K Digestion $ Improved Recovery from FFPE Samples Flourescence History Spin Wash Elute $ Zymo-Spin IIC Ultra-pure DNA Ready for PCR, Sequencing, etc. Flourescence ( ) Zymo Cycles Supplier Q Equivalent amounts of DNA isolated using Zymo and Supplier Q procedures were used for real time PCR analysis. DNA isolated using the Quick-DNA FFPE Kit consistently yielded lower Ct values values as depicted by the amplification curves above. Product Cat. No. Size Quick-DNA FFPE Kit D3067 Learn more and view additional Quick-DNA Kit formats at /dna/quick-dna

7 Quick-DNA Environmental Kits Our environmental purification kits feature unique BashingBead technology, allowing high-recovery of DNA from a wide variety of toughto-lyse environmental samples. These products lead to high-yield and quality DNA suitable for sensitive downstream applications. Our novel inhibitor removal technology, featured in our fecal/soil microbe and plant/seed kits, contains the components needed for efficient removal of polyphenolic compounds that can inhibit downstream enzymatic reactions. Improved Recovery of High-quality DNA from Environmental Samples Quick-DNA Fecal/ Soil Microbe Kit Quick-DNA Fungal/ Zymo Research Bacterial Kit Supplier A Supplier B 1 kb Comparison of DNA yields from rat feces using the QuickDNA Fecal/Soil Microbe Kit and kits from suppliers A and B. Equivalent amounts of feces were processed using each kit and then equal volumes of eluted DNA were analyzed in a 0.8% (w/v) agarose/ ethidium bromide gel. Samples were processed in triplicate. CT No Amplification No Amplification No Amplification No Amplification Supplier A Yeast Spores E. coli Ultra-pure, Inhibitor-free DNA E. coli Fungal and bacterial DNA purification. DNA isolated from Saccharomyces cerevisiae (spores) and E. coli using the Quick-DNA Fungal/Bacterial Kit is high-quality and structurally intact. Equivalent amounts of yeast and bacteria were processed using the Quick-DNA Fungal/Bacterial Kit or the kit from Supplier A. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The size marker is a 1 kb ladder (Zymo Research) Yeast Spores 10% Eluate 35% Eluate The Quick-DNA Environmental Kits provide inhibitor-free DNA even when challenged with extremely inhibitor-rich samples ZymoBIOMICS Quick-DNA Supplier M Supplier P Supplier Q Product Cat. No. Size Quick-DNA Fecal/Soil Microbe Miniprep Kit D6010 Quick-DNA Fungal/Bacterial Miniprep Kit D6005 Quick-DNA Tissue & Insect Miniprep Kit D6016 Quick-DNA Plant/Seed Miniprep Kit D6020 Learn more and view additional Quick-DNA Kit formats at /dna/quick-dna

8 Innovation. Pure & Simple. Since its inception in 1994, Zymo Research has been proudly serving the scientific community by providing innovative, reliable, and high quality research tools at affordable prices. Our vision... The Beauty of Science is to Make Things Simple is now truer than ever. Whether it s epigenetics, DNA, RNA, E. coli, or yeast based research, our philosophy remains the same: To provide the highest quality products in the industry while ensuring they are both simple to use and reliable in their performance. Zymo Research stands on three pillars which form the foundation of our company: Innovation, Quality, and Customer Service. These pillars are fundamental to our culture and ensure our products meet your needs. Innovation Zymo Research is historically recognized for its innovation of high quality nucleic acid purification technologies. Under the branding DNA Purification Made Simple and RNA Purification Made Simple, our technologies are pushing the limits of what is possible with nucleic acid isolation. As The Epigenetics Company, Zymo Research has also received much attention for its rapidly expanding portfolio of epigenetics products and services. It is our objective to develop and provide the most comprehensive set of tools for DNA, RNA, and epigenetic research and analysis available today. Thousands of peer-reviewed scientific publications from researchers around the world feature our technologies. Through innovation, our scientists have made streamlined DNA methylation detection possible, pioneered the micro-elution column for DNA and RNA purification, developed the simplest and the most sophisticated methods for highquality plasmid DNA purification, and patented the first RNA purification directly from Trizol without phase separation among many other leading technologies in the industry. Quality We are committed to quality and guarantee that all of our products and service will meet and exceed your expectations. Our products are constantly evaluated by scientists like you to help ensure their reliability and the highest standard of quality. Customer Service We strive for excellence in how we support your scientific endeavors. We pledge to be honest and responsible for everything we do with you. We will treat you as we would like to be treated. Together, we will build a brighter future. Cat. No. B1001 Version 01