BiOstic Paraffin Removal Reagent

Transcription

1 BiOstic Paraffin Removal Reagent Catalog No. Quantity Instruction Manual F Please recycle Version:

2 Introduction: Use this product in place of xylene prior to the isolation of genomic DNA or RNA from paraffin embedded tissues. The BiOstic Paraffin Removal Reagent is a non-toxic safe alternative to xylene for the removal of wax from FFPE (Formalin Fixed Paraffin Embedded) tissues. *This kit is for research purposes only. Not for diagnostic use. Other Related Products Catalog No. Quantity BiOstic FFPE Tissue DNA Isolation Proteinase K mg 2 ml (20 mg/ml). Equipment Required: Microcentrifuge (16,000 x g) 55 C water bath Pipet (volumes required 1000 l) Vortex Reagents Required but not Included: 100% Ethanol (MO BIO Catalog # ) 2 ml Collection Tubes (MO BIO Catalog # T) Contents: BiOstic Paraffin Removal Reagent, 2 x 25 ml Storage: Store at room temperature (15-30ºC). Precautions: Please wear gloves when using this product. Avoid all skin contact with reagents in this kit. In case of contact wash thoroughly with water. Do not ingest. See Material Safety Data Sheets for emergency procedures in case of accidental ingestion or contact. All MSDS information is available upon request ( ) or on our web site at Reagents labeled flammable should be kept away from open flames and fire. Warning: The BiOstic Paraffin Removal Reagent is flammable. Note: Do NOT use polystyrene disposable serological pipets or other polystyrene plasticware for liquid handling of this reagent. Protocol: 1. Combine one to three FFPE tissue slices (up to 15 mg of tissue) into a single tube. 2. Add of the BiOstic Paraffin Removal Reagent to the tube. Vortex, then incubate for 3 minutes at 55ºC to promote the melting of the wax. Note: After three minutes you will see the tissue floating in the wax remover. 3. Centrifuge full speed (16,000 x g) for 3 minutes to pellet the tissue. 4. Remove the supernatant with pipetting (avoiding the tissue) and add of 100% ethanol. Vortex for 20 seconds to wash and centrifuge full speed (16,000 x g) 3 minutes. Remove the ethanol with pipetting, taking care to avoid the tissue. 2

3 5. Place the at 55ºC for 10 minutes with caps open to evaporate the ethanol. 6. Your tissue is now ready for use in DNA or RNA isolation kits. We recommend the MO BIO BiOstic FFPE Tissue DNA Isolation (MO BIO Catalog # ). Thank you for choosing the BiOstic Paraffin Removal Reagent! 3

4 Contact Information Technical Support: Phone MO BIO Laboratories, Inc. Toll Free , or Fax: Mail: MO BIO Laboratories, Inc, 2746 Loker Ave West, Carlsbad, CA Ordering Information: Direct: Phone MO BIO Laboratories, Inc. Toll Free , or Fax: Mail: MO BIO Laboratories, Inc, 2746 Loker Ave West, Carlsbad, CA For the distributor nearest you, visit our web site at 4

5 Other Quality Products Available from MO BIO Laboratories, Inc. For more product and detailed information go to to request a catalog. DNA Purification and Gel Extraction Catalog No. Quantity Genomic DNA Isolation Continued Catalog No. Quantity PowerClean DNA Clean-Up PowerSoil-htp 96 Well Soil DNA Isolation UltraClean 15 DNA Purification preps UltraClean Soil DNA Isolation UltraClean PCR Clean-Up UltraClean-htp 96 Well Soil DNA Isolation UltraClean-htp 96 Well PCR Clean- Up UltraClean Mega Soil DNA Isolation UltraClean GelSpin DNA Extraction Plasmid DNA Isolation Catalog No. Quantity UltraClean 6 Minute Mini Plasmid Prep UltraClean Standard Mini Plasmid Prep PowerClean DNA Clean-Up UltraClean Fecal DNA Isolation PowerMicrobial Midi DNA Isolation preps PowerMicrobial Maxi DNA Isolation preps UltraClean-htp 96 Well Plasmid Prep UltraClean Microbial DNA Isolation UltraClean Midi Plasmid Prep preps UltraClean-htp 96 Well Microbial DNA Isolation UltraClean Maxi Plasmid Prep preps PowerPlant DNA Isolation UltraClean Endotoxin-Free Mini Plasmid Prep UltraClean Plant DNA Isolation UltraClean Endotoxin-Free Midi Plasmid Prep UltraClean-htp 96 Well Plant DNA Isolation UltraClean Endotoxin-Free Maxi Plasmid Prep UltraClean Tissue & Cells DNA Isolation UltraClean Endotoxin Removal kit UltraClean-htp 96 Well Tissue DNA Isolation UltraClean Endotoxin-Free Ethanol kit UltraClean Blood DNA Isolation Precipitation (Non-Spin) UltraClean Endotoxin Removal ml UltraClean Blood DNA Isolation kit Reagent (Processes 1,000 ml of Blood) Endotoxin-Free Sodium Chloride ml UltraClean Blood DNA Isolation Plus RNase (Processes 1,000 ml of Blood) kit Endotoxin-Free Centrifuge Tubes each/2 ml 50 each/15 ml 25 each/50 ml UltraClean BloodSpin DNA Isolation RNA Isolation Catalog No. Quantity UltraClean-htp 96 Well BloodSpin DNA Isolation RNA PowerSoil Total RNA Isolation preps UltraClean Mega BloodSpin DNA Isolation UltraClean Microbial RNA Isolation UltraClean Forensic DNA Isolation UltraClean Tissue RNA Isolation UltraClean Water DNA Isolation (0.45µm filters) UltraClean Plant RNA Isolation preps UltraClean Water DNA Isolation (0.22 µm filters) UltraClean Water DNA Isolation Genomic DNA Isolation Catalog No. Quantity (no filters) PowerMax Soil DNA Isolation PowerSoil DNA Isolation NF NF 10 isolations 20 isolations 25 preps 25 preps 25 preps 5

6 Other Quality Products Available from MO BIO Laboratories, Inc. For more product and detailed information go to to request a catalog. Microbiological Culture Media Catalog No. Quantity TB DRY Powder Growth Media LB Broth Powder Growth Media, ph 7 LB Agar Powder Growth Media, ph 7 LB Broth (Lennox) Powder Growth Media, ph 7 LB Agar (Lennox) Powder Growth Media, ph 7 Soybean-Casein Digest Medium (TSB), USP Soybean-Casein Digest Agar Medium (TSA), USP Yeast Extract Tryptone Agar, Bacteriological Grade Other Reagents and Lab Accessories Continued Catalog No. Quantity Ethidium Bromide Destaining Tea bags Bags Bromophenol Blue Gel Loading Buffer Bromophenol Blue/Xylene Cyanol Gel Loading Buffer TAE Buffer, 50X (Tris-acetate-EDTA) TBE Buffer, 10X (Tris-borate-EDTA) RNase-Free Gloves 1555-XS 1555-S 1555-M 1555-L UltraClean Lab Cleaner OmniTaq DNA Polymerase Enzyme OmniTaq DNA Polymerase 2x Master Mix Omni KlenTaq DNA Polymerase Enzyme x 5 x 100 ml 500 ml 1 liter 100 ml 500 ml 1 liter 250 ml squeeze bottle 500 ml spray bottle liter bottle reactions (10 U/µl) reactions (5 x 1.25 ml/tube) reactions (25 U/µl) Omni KlenTaq DNA Polymerase 2x Other Reagents and Lab Master Mix Accessories Catalog No. Quantity 20 bp DNA Ladder µg DNase (RNase-Free) mg 100 bp DNA Ladder µg Proteinase K kb DNA Ladder µg Ribonuclease A UltraClean Agarose, Molecular Biology Grade UltraClean MS-8 Agarose UltraClean Forensic Agarose UltraClean Low Melt Agarose UltraClean Low Melt Sieve Agarose Ethidium Bromide Solution ml reactions (5 x 1.25 ml/tube) PCR Water Molecular Biology Grade Water DEPC Treated Water Endotoxin-Free Water mg 2 ml (20 mg/ml) (25 mg/ml) 5 ml (25 mg/ml) 5 x 10 x 10 ml bottle 200 ml 5 x 200 ml 200 ml 5 x 200 ml 10 ml 50 ml 100 ml 500 ml 6

7 Other Quality Products Available from MO BIO Laboratories, Inc. For more product and detailed information go to to request a catalog. Instrumentation and Accessories Catalog No. Quantity Instrumentation and Accessories Continued Catalog No. Quantity Precellys 24 Homogenizer, 120V unit Vortex Adapter, holds 2 (50 ml) V unit Ceramic Bead Tubes, 1.4 mm bead Vortex Adapter, holds 24 ( ml) V unit Ceramic Bead Tubes, 2.8 mm bead Power Supply w/timer, (120V) unit Glass Bead Tubes, 0.5 mm bead Power Supply w/timer, (220V) unit Glass Bead Tubes, 0.1 mm bead Polycarbonate Single-sided Comb Metal Bead Tubes, 2.38 mm bead Polycarbonate Dual-sided Comb ml Tough Tubes with Cap x 2.0 ml Tough Tubes with Cap 1000 x 2.0 ml Tough Tubes with Cap Carbide Bead Tubes, 0.25 mm x 0.5 ml Garnet Bead Tubes, 0.15 mm x 0.5 ml Garnet Bead Tubes, 0.70 mm x 2 ml 2 ml Collection Tubes T T T ml Screw Cap Tubes E 200 & caps Teflon Single-sided Comb mm x 3 well 1 mm x 8 well 1 mm x 10 well 1 mm x 12 well 1 mm x 8 1 mm x 10 2 mm x 8 2 mm x 10 1 mm x 3 well 1 mm x 8 well 1 mm x 10 well 1 mm x 12 well Teflon Dual-sided Comb mm x 8 1 mm x 10 2 mm x 8 2 mm x 10 Mini Horizontal Gel System each Mini Horizontal Gel Caster, 1 place each Mini Horizontal Gel Caster, 3 place each Mini Horizontal Gel Tray each 15 ml Collection Tubes T Well Plate Shaker (120V) unit 50 ml Centrifuge Tubes T Well Plate Shaker (220V) unit Spin Filters SF 50 filters in Plate Adapter Set set SF SF 100 filters in 250 filters in Endotoxin-Free Centrifuge Tubes each/2 ml Tube Adapter Set set each/15 ml 25 each/50 ml 15 ml Midi Spin Filters SF 25 spin filters Vacuum Pump (120V) unit Vortex-Genie 2 Vortex (120V) V 1 unit Vacuum Pump (220V) unit Vortex-Genie 2 Vortex (220V) V unit UltraVac Manifold unit Vortex Adapter, holds 12 ( ml) V1 1 unit Vortex Adapter, holds 4 (15 ml) V unit 7