Stocking Up? Reagents and Supplies Quick Reference Guide. discover.bio-rad.com

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1 Stocking Up? Reagents and Supplies Quick Reference Guide discover.bio-rad.com

2 Chromatography Selection Chart Ion Exchange Chromatography Prepacked Columns/Cartridges Bottled Media General biomolecule purification Applicable to all antibody purifications High capacity Good capture step Separation by charge UNO Q UNO S Econo-Pac High Q Econo-Pac UNOsphere S Econo-Pac UNOsphere Q Econo-Pac DEAE Econo-Pac High S Econo-Pac CM Macro-Prep Q Macro-Prep S Macro-Prep High Q Macro-Prep High S Macro-Prep CM Macro-Prep DEAE UNOsphere Q UNOsphere S Size Exclusion Chromatography Prepacked Columns/Cartridges Bottled Media Molecular weight determination Applicable to all antibody purifications High resolution Separation by molecular size Desalting or buffer exchange Econo-Pac P-6 Bio-Spin P-6 Micro Bio-Spin P-6 Bio-Sil /Bio-Silect Bio-Gel P Hydroxyapatite Chromatography Prepacked Columns/Cartridges Bottled Media Unique selectivity Very high purity can often be obtained in one step Alternative to protein A for antibody purification Bio-Scale CHT-I Econo-Pac CHT-I Econo-Pac CHT-II Prepacked Columns/Cartridges Ready-to-use supports CHT ceramic Bio-Gel HT gel hydroxyapatite Type I, Bio-Gel HTP gel, 40, 80 µm CHT ceramic hydroxyapatite Type II,, 40, 80 µm Bottled Media Ready-to-Use Affinity Chromatography High selectivity High purity in a single step Excellent capture technique Separation by biological interaction Econo-Pac protein A Econo-Pac Blue Econo-Pac DEAE Blue Activated coupling supports Affi-Prep protein A Affi-Gel protein A Affi-Gel Blue DEAE Affi-Gel Blue Affi-Gel boronate Affi-Prep polymyxin Bottled Media Activated Affi-Prep Hz Affi-Gel Hz Affi-Gel Affi-Prep Affi-Gel Affi-Gel boronate Affi-Gel 2 Hydrophobic Interaction Chrom. Prepacked Columns/Cartridges Bottled Media Higher-resolution alternative to ammonium sulfate precipitation Gentle elution conditions Separation by hydrophobicity of biomolecules Econo-Pac t-butyl HIC Econo-Pac methyl HIC Macro-Prep t-butyl Macro-Prep methyl

3 Chromatography Media and Columns Size/ Storage Ion Exchange Bottled Media Macro-Prep Q Support* ml Ambient Macro-Prep S Support* ml Ambient Macro-Prep High Q Support** ml Ambient Macro-Prep High S Support** ml Ambient 6-00 Macro-Prep DEAE Support** ml Ambient Macro-Prep CM Support** ml Ambient 6-01 UNOsphere Q Support*** ml Ambient UNOsphere S Support*** ml Ambient * Available in ml, 0 ml, 1 L, and 5 L ** Available in ml, ml, 0 ml, 5 L, and L *** Available in ml, ml, 0 ml, and L Prepacked Ion Exchange Cartridges Macro-Prep High Q Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient Macro-Prep High Q Support, 5 x 5 ml Econo-Pac cartridges 5 cartridges Ambient Macro-Prep High Q Support, 5 x 1 ml Econo-Pac cartridges 5 cartridges Ambient Macro-Prep High S Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient Macro-Prep High S Support, 5 x 5 ml Econo-Pac cartridges 5 cartridges Ambient Macro-Prep High S Support, 5 x 1 ml Econo-Pac cartridges 5 cartridges Ambient Macro-Prep CM Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient Macro-Prep CM Support, 5 x 1 ml Econo-Pac cartridges 5 cartridges Ambient Macro-Prep CM Support, 5 x 5 ml Econo-Pac cartridges 5 cartridges Ambient UNOsphere Q Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient UNOsphere Q Support, 5 x 5 ml Econo-Pac cartridge 5 cartridges Ambient UNOsphere Q Support, 5 x 1 ml Econo-Pac cartridge 5 cartridges Ambient UNOsphere S Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient UNOsphere S Support, 5 x 5 ml Econo-Pac cartridge 5 cartridges Ambient UNOsphere S Support, 5 x 1 ml Econo-Pac cartridge 5 cartridges Ambient Macro-Prep DEAE Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient Macro-Prep DEAE Support, 5 x 5 ml Econo-Pac cartridge 5 cartridges Ambient Macro-Prep DEAE Support, 5 x 1 ml Econo-Pac cartridge 5 cartridges Ambient Prepacked Ion Exchange Columns UNO Q1 Column, 7 x 35 mm 1 column Ambient UNO Q6 Column, 12 x 53 mm 1 column Ambient UNO Q12 Column, x 68 mm 1 column Ambient UNO Q Polishing Column, 4.6 x mm 1 column Ambient UNO Q1R Column, 7 x 35 mm 1 column Ambient UNO Q6R Column, 12 x 53 mm 1 column Ambient 7-00 UNO Q12R Column, x 68 mm 1 column Ambient UNO S1 Column, 7 x 35 mm 1 column Ambient UNO S6 Column, 12 x 53 mm 1 column Ambient 7-00 UNO S12 Column, x 68 mm 1 column Ambient UNO S Polishing Column, 4.6 x mm 1 column Ambient Size/ Storage UNO S1R Column, 7 x 35 mm 1 column Ambient UNO S6R Column, 12 x 53 mm 1 column Ambient UNO S12R Column, x 68 mm 1 column Ambient Size Exclusion Bottled Media Bio-Gel P-2 Gel, fine g Ambient 1-41 Bio-Gel P-2 Gel, fine 0 g Ambient Bio-Gel P-2 Gel, extra fine g Ambient 1-41 Bio-Gel P-4 Gel, medium g Ambient Bio-Gel P-4 Gel, fine g Ambient Bio-Gel P-4 Gel, extra fine g Ambient Bio-Gel P-6 Gel, medium g Ambient Bio-Gel P-6 Gel, fine g Ambient Bio-Gel P-6 Gel, extra fine g Ambient Bio-Gel P-6 DG Gel g Ambient Bio-Gel P- Gel, medium g Ambient Bio-Gel P- Gel, fine g Ambient 1-41 Bio-Gel P-30 Gel, medium g Ambient 1-44 Bio-Gel P-30 Gel, fine g Ambient Bio-Gel P-60 Gel, medium g Ambient Bio-Gel P-60 Gel, fine g Ambient Bio-Gel P- Gel, medium g Ambient Bio-Gel P- Gel, fine g Ambient Prepacked Size Exclusion (SEC) Columns and Cartridges 732- Econo-Pac DG Desalting Columns 30 columns Ambient Bio-Gel P-6 Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient Bio-Gel P-6 Support, 5 x 5 ml Econo-Pac cartridges 5 cartridges Ambient Prepacked Size Exclusion (SEC) HPLC Stainless Steel Columns Bio-Sil SEC 1-5 Column, 300 x 7.8 mm 1 column Ambient Bio-Sil SEC 2-5 Column, 300 x 7.8 mm 1 column Ambient Bio-Sil SEC Column, 300 x 7.8 mm 1 column Ambient Bio-Sil SEC 1 Guard, 80 x 7.8 mm 1 guard Ambient Bio-Sil SEC 2 Guard, 80 x 7.8 mm 1 guard Ambient Bio-Sil SEC 400 Guard, 80 x 7.8 mm 1 guard Ambient Prepacked Size Exclusion (SEC) HPLC PEEK Biocompatible Columns 1-04 Bio-Silect SEC 1-5 Column, 300 x 7.8 mm 1 column Ambient Bio-Silect SEC 2-5 Column, 300 x 7.8 mm 1 column Ambient Bio-Silect SEC Column, 300 x 7.8 mm 1 column Ambient Bio-Silect SEC 1 Guard, x 7.8 mm 1 guard Ambient Bio-Silect SEC 2 Guard, x 7.8 mm 1 guard Ambient Bio-Silect SEC 400 Guard, x 7.8 mm 1 guard Ambient (continues) Chromatography Media and Columns

4 Chromatography Media and Columns (cont.) Size/ Storage Hydroxyapatite Bottled Media* 7-00 CHT Ceramic Hydroxyapatite, Type I, µm g Ambient CHT Ceramic Hydroxyapatite, Type I, 40 µm g Ambient CHT Ceramic Hydroxyapatite, Type I, 80 µm g Ambient 7-00 CHT Ceramic Hydroxyapatite, Type II, µm g Ambient CHT Ceramic Hydroxyapatite, Type II, 40 µm g Ambient CHT Ceramic Hydroxyapatite, Type II, 80 µm g Ambient Prepacked Hydroxyapatite Cartridges CHT Type I Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient CHT Type I Support, 5 x 1 ml Econo-Pac cartridges 5 cartridges Ambient CHT Type I Support, 5 x 5 ml Econo-Pac cartridges 5 cartridges Ambient CHT Type II Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient CHT Type II Support, 5 x 1 ml Econo-Pac cartridges 5 cartridges Ambient CHT Type II Support, 5 x 5 ml Econo-Pac cartridges 5 cartridges Ambient Prepacked Hydroxyapatite Columns Bio-Scale CHT2 Type I Column, 7 x 52 mm 1 column Ambient Bio-Scale CHT5 Type I Column, x 64 mm 1 column Ambient 1-00 Bio-Scale CHT Type I Column, 12 x 88 mm 1 column Ambient Bio-Scale CHT Type I Column, x 113 mm 1 column Ambient Top-Off Resin Kit CHT Type I, 1 ml 1 kit Ambient Hydrophobic Interaction Bottled Media Macro-Prep Methyl HIC Support ml Ambient Macro-Prep t-butyl HIC Support ml Ambient Prepacked Hydrophobic Interaction Cartridges Macro-Prep Methyl HIC Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient Macro-Prep Methyl HIC Support, 5 x 1 ml Econo-Pac cartridges 5 cartridges Ambient Macro-Prep Methyl HIC Support, 5 x 5 ml Econo-Pac cartridges 5 cartridges Ambient Macro-Prep t-butyl HIC Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient Macro-Prep t-butyl HIC Support, 5 x 5 ml Econo-Pac cartridges 5 cartridges Ambient Macro-Prep t-butyl HIC Support, 5 x 1 ml Econo-Pac cartridges 5 cartridges Ambient Activated Affinity Bottled Media Affi-Gel Gel ml C Affi-Prep Gel ml C Affi-Gel Gel ml C Affi-Gel /Affi-Gel Combination 2 x ml each C * Also available in g, 1 kg, and 5 kg Size/ Storage Ready-to-Use Affinity Media Affi-Gel Hz Hydrazide Gel ml 4 C Affi-Gel Hz Immunoaffinity Kit 1 kit 4 C Affi-Prep Hz Hydrazide Gel ml 4 C 3-64 Affi-Gel Protein A Agarose ml 4 C 3-69 Affi-Gel Protein A MAPS II Kit 1 kit 4 C Affi-Prep Protein A Support ml 4 C Affi-Prep MAPS II Kit 1 kit 4 C Affi-Gel Heparin Gel 40 ml 4 C Affi-Gel Blue Gel, mesh ml 4 C Affi-Gel Blue Gel, 0 mesh ml 4 C CM Affi-Gel Blue Gel ml 4 C DEAE Affi-Gel Blue Gel ml 4 C 6-00 Affi-Prep Polymyxin Support ml 4 C Prepacked Affinity Columns and Cartridges DEAE Affi-Gel Blue Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient DEAE Affi-Gel Blue Support, 5 x 5 ml Econo-Pac cartridges 5 cartridges Ambient Affi-Prep Protein A Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient Affi-Prep Protein A Support, 5 x 5 ml Econo-Pac cartridges 5 cartridges Ambient Affi-Gel Blue Support, 1 x 5 ml Econo-Pac cartridge 1 cartridge Ambient Affi-Gel Blue Support, 5 x 5 ml Econo-Pac cartridges 5 cartridges Ambient Econo-Pac Protein A Columns 5 columns Ambient Chromatography Standards Protein Standard for Anion Exchange 6 vials 4 C Protein Standard for Cation Exchange 6 vials 4 C Protein Standard for Size Exclusion Standard 6 vials 4 C Protein Standard for Hydrophobic Interaction 6 vials 4 C Empty Chromatography Columns 731- Poly-Prep Chromatography Columns, empty columns Ambient 732- Econo-Pac Chromatography Columns, empty columns Ambient Bio-Scale MT2 Column, 7 x 52 mm 1 column Ambient Bio-Scale MT5 Column, x 64 mm 1 column Ambient Bio-Scale MT Column, 12 x 88 mm 1 column Ambient Bio-Scale MT Column, x 113 mm 1 column Ambient

5 Chromatography Media and Columns (cont.) Size/ Storage Econo-Column Chromatography Columns* Econo-Column Funnels, 2 ml 5 funnels Ambient 7-06 Econo-Column Chromatography Column, 0.5 x 5 cm 5 columns Ambient Econo-Column Chromatography Column, 0.5 x cm 5 columns Ambient Econo-Column Chromatography Column, 0.7 x 5 cm 5 columns Ambient Econo-Column Chromatography Column, 0.7 x cm 5 columns Ambient Econo-Column Chromatography Column, 0.7 x cm 5 columns Ambient Econo-Column Chromatography Column, 0.7 x cm 5 columns Ambient 7-6 Econo-Column Chromatography Column, 1 x 5 cm 5 columns Ambient 7-11 Econo-Column Chromatography Column, 1 x cm 5 columns Ambient 7-12 Econo-Column Chromatography Column, 1 x cm 2 columns Ambient 7-21 Econo-Column Chromatography Column, 1 x cm 5 columns Ambient 7-22 Econo-Column Chromatography Column, 1 x cm 2 columns Ambient 7-31 Econo-Column Chromatography Column, 1 x 30 cm 5 columns Ambient 7-16 Econo-Column Chromatography Column, 1.5 x 5 cm 5 columns Ambient 7-11 Econo-Column Chromatography Column, 1.5 x cm 5 columns Ambient 7-12 Econo-Column Chromatography Column, 1.5 x cm 2 columns Ambient 7-16 Econo-Column Chromatography Column, 1.5 x cm 5 columns Ambient 7-17 Econo-Column Chromatography Column, 1.5 x cm 2 columns Ambient 7-21 Econo-Column Chromatography Column, 1.5 x cm 5 columns Ambient 7-22 Econo-Column Chromatography Column, 1.5 x cm 2 columns Ambient 7-31 Econo-Column Chromatography Column, 1.5 x 30 cm 5 columns Ambient 7-32 Econo-Column Chromatography Column, 1.5 x 30 cm 2 columns Ambient 7-52 Econo-Column Chromatography Column, 1.5 x cm 2 columns Ambient 7-76 Econo-Column Chromatography Column, 1.5 x cm 2 columns Ambient 7-11 Econo-Column Chromatography Column, 2.5 x cm 5 columns Ambient 7-12 Econo-Column Chromatography Column, 2.5 x cm 2 columns Ambient 7-21 Econo-Column Chromatography Column, 2.5 x cm 5 columns Ambient 7-22 Econo-Column Chromatography Column, 2.5 x cm 2 columns Ambient 7-32 Econo-Column Chromatography Column, 2.5 x 30 cm 2 columns Ambient 7-51 Econo-Column Chromatography Column, 2.5 x cm 2 columns Ambient 7-11 Econo-Column Chromatography Column, 5 x cm 1 column Ambient 7-21 Econo-Column Chromatography Column, 5 x cm 1 column Ambient 7-31 Econo-Column Chromatography Column, 5 x 30 cm 1 column Ambient Jacketed Econo-Column Columns 7-68 Standard Jacketed Column, 0.7 x cm 1 column Ambient Standard Jacketed Column, 1 x cm 1 column Ambient Standard Jacketed Column, 1 x 30 cm 1 column Ambient 7-61 Standard Jacketed Column, 1.5 x cm 1 column Ambient 7-61 Open-Ended Jacketed Cloumn, with 2 flow adaptors, 1 x 30 cm 1 column Ambient Flow Adaptors Flow Adaptor, 1.0 cm column ID, 1 7 cm functional length 1 adaptor Ambient Flow Adaptor, 1.0 cm column ID, 1 14 cm functional length 1 adaptor Ambient Flow Adaptor, 1.5 cm column ID, 1 14 cm functional length 1 adaptor Ambient Flow Adaptor, 2.5 cm column ID, 1 14 cm functional length 1 adaptor Ambient Flow Adaptor, 5.0 cm column ID, 1 14 cm functional length 1 adaptor Ambient Econo-Pac Flow Adaptor, 1.5 cm column ID 1 adaptor Ambient * For longer column lengths, refer to the Life Science Research Products catalog. Chromatography Media and Columns

6 ReadyStrip IPG Strip Selection Chart ph Gradients Relative Focusing Power* ReadyStrip IEF Buffer cm 11 cm 17 cm 18 cm 24 cm Broad Range 3 1.0x 1.6x 2.4x 2.6x 3.4x 3 nonlinear (NL) 1.0x 1.6x 2.4x 2.6x 3.4x Narrow Range x 3.7x 5.7x 6.0x 8.0x x 3.7x 5.7x 6.0x 8.0x 7 2.3x 3.7x 5.7x 6.0x x 3.7x 5.7x 6.0x 8.0x Micro Range x 9.2x 14.2x.0x x 9.2x 14.2x.0x x 9.2x 14.2x.0x x 5.5x 8.5x 9.0x * Relative Focusing Power Relative focusing power expresses the enhanced resolution expected in the first dimension when using IPG strips of different lengths or ph ranges. The 7 cm ph 3 IPG strip is arbitrarily assigned a baseline focusing power of 1.0 in order to calculate the relative focusing powers of the other strips, as shown in the table above. The calculations are done as follows: When two strips have the same ph range but differ in length the ratio of the strip lengths gives the relative focusing power of the longer strip. Thus, compared to a 7 cm ph 3 strip, the 11 cm ph 3 strip has a relative focusing power of 11 cm/7 cm = 1.6 When two strips have the same length but differ in ph range the ratio of the ph ranges (in number of ph units) gives the relative focusing power of the strip with the smaller range. Thus, compared to a 7 cm ph 3 strip, the 7 cm ph 5 8 strip has a relative focusing power of (7 ph units)/(3 ph units) = 2.3

7 ReadyStrip IPG Strips and Related Buffers 7 cm IPG Strips ReadyStrip IPG Strips, ph 3, 7 cm 12 strips C ReadyStrip IPG Strips, ph 4 7, 7 cm 12 strips C ReadyStrip IPG Strips, ph 3 nonlinear, 7 cm 12 strips C ReadyStrip IPG Strips, ph 3 6, 7 cm 12 strips C ReadyStrip IPG Strips, ph 5 8, 7 cm 12 strips C ReadyStrip IPG Strips, ph 7, 7 cm 12 strips C ReadyStrip IPG Strips, ph , 7 cm 12 strips C ReadyStrip IPG Strips, ph , 7 cm 12 strips C ReadyStrip IPG Strips, ph , 7 cm 12 strips C ReadyStrip IPG Strips, ph , 7 cm 12 strips C 11 cm IPG Strips ReadyStrip IPG Strips, ph 3, 11 cm 12 strips C 163- ReadyStrip IPG Strips, ph 4 7, 11 cm 12 strips C ReadyStrip IPG Strips, ph 3 nonlinear, 11 cm 12 strips C ReadyStrip IPG Strips, ph 3 6, 11 cm 12 strips C ReadyStrip IPG Strips, ph 5 8, 11 cm 12 strips C ReadyStrip IPG Strips, ph 7, 11 cm 12 strips C ReadyStrip IPG Strips, ph , 11 cm 12 strips C 163- ReadyStrip IPG Strips, ph , 11 cm 12 strips C ReadyStrip IPG Strips, ph , 11 cm 12 strips C ReadyStrip IPG Strips, ph , 11 cm 12 strips C 17 cm IPG Strips ReadyStrip IPG Strips, ph 3, 17 cm 12 strips C ReadyStrip IPG Strips, ph 4 7, 17 cm 12 strips C ReadyStrip IPG Strips, ph 3 nonlinear, 17 cm 12 strips C 163- ReadyStrip IPG Strips, ph 3 6, 17 cm 12 strips C ReadyStrip IPG Strips, ph 5 8, 17 cm 12 strips C ReadyStrip IPG Strips, ph 7, 17 cm 12 strips C 163- ReadyStrip IPG Strips, ph , 17 cm 12 strips C ReadyStrip IPG Strips, ph , 17 cm 12 strips C ReadyStrip IPG Strips, ph , 17 cm 12 strips C ReadyStrip IPG Strips, ph , 17 cm 12 strips C 18 cm IPG Strips ReadyStrip IPG Strips, ph 3, 18 cm 12 strips C ReadyStrip IPG Strips, ph 3 nonlinear, 18 cm 12 strips C ReadyStrip IPG Strips, ph 4 7, 18 cm 12 strips C ReadyStrip IPG Strips, ph 3 6, 18 cm 12 strips C ReadyStrip IPG Strips, ph 5 8, 18 cm 12 strips C 163- ReadyStrip IPG Strips, ph 7, 18 cm 12 strips C ReadyStrip IPG Strips, ph , 18 cm 12 strips C ReadyStrip IPG Strips, ph , 18 cm 12 strips C ReadyStrip IPG Strips, ph , 18 cm 12 strips C ReadyStrip IPG Strips, ph , 18 cm 12 strips C 24 cm IPG Strips ReadyStrip IPG Strips, ph 3, 24 cm 12 strips C ReadyStrip IPG Strips, ph 3 nonlinear, 24 cm 12 strips C ReadyStrip IPG Strips, ph 4 7, 24 cm 12 strips C ReadyStrip IPG Strips, ph 3 6, 24 cm 12 strips C ReadyStrip IPG Strips, ph 5 8, 24 cm 12 strips C Bio-Lyte IEF Buffers x Bio-Lyte 3 Ampholyte 1 ml 4 C x ReadyStrip 7 Buffer 1 ml 4 C x ReadyStrip Buffer 1 ml 4 C x ReadyStrip Buffer 1 ml 4 C x ReadyStrip Buffer 1 ml 4 C x ReadyStrip Buffer 1 ml 4 C Rehydration/Sample Buffers ReadyPrep 2-D Starter Kit Rehydration/Sample Buffer 1 ml 4 C ReadyPrep Rehydration/Sample Buffer 1 ml Ambient Equilibration Buffers ReadyPrep 2-D Starter Kit Rehydration/Sample Buffer I, with DTT ml 4 C ReadyPrep 2-D Starter Kit Rehydration/Sample Buffer II, without DTT ml 4 C Polyacrylamide IPG Strips and Precast Gels

8 Gel Migration Charts Ready Gel Precast Gels Tris-HCI Tris-Tricine IEF Zymogram TBE TBE-Urea 5% 7.5% % 12% % 18% 4 % 4 % 8 16% % 16.5% % ph 3 ph 5 8 % 12% 5% % % 4 % 5% % % XC BPB XC XC BPB XC BPB BPB Tris/glycine/SDS buffer Tris/Tricine/ SDS buffer IEF cathode buffer, IEF anode buffer Tris/glycine/ SDS buffer TBE buffer TBE buffer Criterion Precast Gels Tris-HCI Bis-Tris Tris-Tricine IEF Zymogram TBE TBE-Urea 5% 7.5% % 12.5% % 18% 4 % 4 % 8 16% %.5 14% % 12% 4 12% % 12% 4 12% 7% 3 8% 16.5% % ph 3 ph 5 8 % Gelatin % Casein 5% % % 4 % 5% % % Tris/glycine/SDS buffer XT MES buffer XT MOPS buffer XT Tricine buffer Tris/Tricine/ SDS buffer IEF cathode buffer, IEF anode buffer Tris/glycine/ SDS buffer TBE buffer TBE buffer

9 Criterion XT Precast Gels Criterion Precast Gels Shelf Life Criterion XT Bis-Tris Gels Criterion XT Bis-Tris Gel, %, 12+2 well, 45 µl 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, %, 18-well, 30 µl 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, %, 26-well, µl 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, %, prep+2 well, 11 cm IPG strip 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, %, prep+2 well, 11 cm IPG strip 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, %, IPG well, 11 cm IPG strip 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, 12%, 12+2 well, 45 µl 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, 12%, 18-well, 30 µl 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, 12%, 26-well, µl 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, 12%, prep+2 well, 800 µl 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, 12%, IPG+1 well, 11 cm IPG strip 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, 12%, IPG well, 11 cm IPG strip 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, 4 12%, 12+2 well, 45 µl 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, 4 12%, 18-well, 30 µl 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, 4 12%, 26-well, µl 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, 4 12%, prep+2 well, 800 µl 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, 4 12%, IPG+1 well, 11 cm IPG strip 1 gel Ambient 12 mo Criterion XT Bis-Tris Gel, 4 12%, IPG well, 11 cm IPG strip 1 gel Ambient 12 mo Criterion XT Tris-Acetate Gels Criterion XT Tris-Acetate Gel, 3 8%, 12+2 well, 45 µl 1 gel 4 C 8 mo Criterion XT Tris-Acetate Gel, 3 8%, 18-well, 30 µl 1 gel 4 C 8 mo Criterion XT Tris-Acetate Gel, 3 8%, 26-well, µl 1 gel 4 C 8 mo Criterion XT Tris-Acetate Gel, 3 8%, prep+2 well, 800 µl 1 gel 4 C 8 mo Criterion XT Tris-Acetate Gel, 3 8%, IPG+1 well, 11 cm IPG strip 1 gel 4 C 8 mo Criterion XT Tris-Acetate Gel, 3 8%, IPG well, 11 cm IPG strip 1 gel 4 C 8 mo Criterion XT Tris-Acetate Gel, 7%, 12+2 well, 45 µl 1 gel 4 C 8 mo Criterion XT Tris-Acetate Gel, 7%, 18-well, 30 µl 1 gel 4 C 8 mo Criterion XT Tris-Acetate Gel, 7%, 26-well, µl 1 gel 4 C 8 mo Criterion XT Tris-Acetate Gel, 7%, prep+2 well, 800 µl 1 gel 4 C 8 mo Criterion XT Tris-Acetate Gel, 7%, IPG+1 well, 11 cm IPG strip 1 gel 4 C 8 mo Criterion XT Tris-Acetate Gel, 7%, IPG well, 11 cm IPG strip 1 gel 4 C 8 mo Criterion XT Buffers and Reagents XT MOPS Running Buffer, x 0 ml Ambient XT MES Running Buffer, x 0 ml Ambient XT Tricine Running Buffer, x 0 ml Ambient XT Sample Buffer, 4x ml Ambient XT Reducing Agent, x 1 ml Ambient XT MOPS Buffer Kit 1 kit Ambient XT MES Buffer Kit 1 kit Ambient XT Tricine Buffer Kit 1 kit Ambient Shelf Life Criterion Tris-HCl Gels Criterion Tris-HCl Gel, 5%, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 5%, 18-well, 30 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 5%, 26-well, µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 5%, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 7.5%, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 7.5%, 18-well, 30 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 7.5%, 26-well, µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 7.5%, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 18-well, 30 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 26-well, µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 11 cm IPG well 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 12.5%, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 12.5%, 18-well, 30 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 12.5%, 26-well, µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 12.5%, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 12.5%, 11 cm IPG well 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 18-well, 30 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 26-well, µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 18%, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 18%, 18-well, 30 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 18%, 26-well, µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 18%, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 4 %, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 4 %, 18-well, 30 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 4 %, 26-well, µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 4 %, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 4 %, 11 cm IPG well 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 4 %, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 4 %, 18-well, 30 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 4 %, 26-well, µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 4 %, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 4 %, 11 cm IPG well 1 gel 4 C 12 wk (continues) Polyacrylamide IPG Strips and Precast Gels

10 Criterion Precast Gels (cont.) Shelf Life Criterion Tris-HCl Gels (cont.) Criterion Tris-HCl Gel, 8 16%, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 8 16%, 18-well, 30 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 8 16%, 26-well, µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 8 16%, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 8 16%, 11 cm IPG well 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 18-well, 30 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 26-well, µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 11 cm IPG well 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 11 cm IPG+1 well 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 12.5%, 11 cm IPG+1 well 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 4 %, 11 cm IPG+1 well 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 4 %, 11 cm IPG+1 well 1 gel 4 C 12 wk Criterion Tris-HCl Gel, 8 16%, 11 cm IPG+1 well 1 gel 4 C 12 wk Criterion Tris-HCl Gel,.5 14%, 11 cm IPG+1 well 1 gel 4 C 12 wk Criterion Tris-HCl Gel, %, 11 cm IPG+1 well 1 gel 4 C 12 wk Criterion TBE Gels Criterion TBE Gel, 5%, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, 18-well, 30 µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, 26-well, µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, 18-well, 30 µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, 26-well, µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, 18-well, 30 µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, 26-well, µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, 18-well, 30 µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, 26-well, µl 1 gel 4 C 12 wk Criterion TBE Gel, 5%, prep+2 well, 800 µl 1 gel 4 C 12 wk Shelf Life Criterion Tris-Tricine/Peptide Gels Criterion Tris-Tricine/Peptide Gel, 16.5%, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion Tris-Tricine/Peptide Gel, 16.5%, 18-well, 30 µl 1 gel 4 C 12 wk Criterion Tris-Tricine/Peptide Gel, 16.5%, 26-well, µl 1 gel 4 C 12 wk Criterion Tris-Tricine/Peptide Gel, 16.5%, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion Tris-Tricine/Peptide Gel, %, 12+2 well, 45 µl 1 gel 4 C 12 wk Criterion Tris-Tricine/Peptide Gel, %, 18-well, 30 µl 1 gel 4 C 12 wk Criterion Tris-Tricine/Peptide Gel, %, 26-well, µl 1 gel 4 C 12 wk Criterion Tris-Tricine/Peptide Gel, %, prep+2 well, 800 µl 1 gel 4 C 12 wk Criterion IEF Gels Criterion IEF Gel, ph 3, 12+2 well, 45 µl 1 gel 4 C 24 wk Criterion IEF Gel, ph 3, 18-well, 30 µl 1 gel 4 C 24 wk Criterion IEF Gel, ph 3, 26-well, µl 1 gel 4 C 24 wk Criterion IEF Gel, ph 5 8, prep+2 well, 800 µl 1 gel 4 C 24 wk Criterion IEF Gel, ph 5 8, 12+2 well, 45 µl 1 gel 4 C 24 wk Criterion IEF Gel, ph 5 8, 18-well, 30 µl 1 gel 4 C 24 wk Criterion IEF Gel, ph 5 8, 26-well, µl 1 gel 4 C 24 wk Criterion IEF Gel, ph 5 8, prep+2 well, 800 µl 1 gel 4 C 24 wk Criterion Zymogram Gels Criterion Zymogram Gel, %, gelatin, 12+2 well, 45 µl 1 gel 4 C 8 wk Criterion Zymogram Gel, %, gelatin, 18-well, 30 µl 1 gel 4 C 8 wk Criterion Zymogram Gel, %, gelatin, 26-well, µl 1 gel 4 C 8 wk Criterion Zymogram Gel, 12.5%, casein, 12+2 well, 45 µl 1 gel 4 C 8 wk Criterion Zymogram Gel, 12.5%, casein, 18-well, 30 µl 1 gel 4 C 8 wk Criterion Zymogram Gel, 12.5%, casein, 26-well, µl 1 gel 4 C 8 wk Criterion TBE-Urea Gels Criterion TBE-Urea Gel, 5%, 12+2 well, 45 µl 1 gel 4 C 8 wk Criterion TBE-Urea Gel, 5%, 18-well, 30 µl 1 gel 4 C 8 wk Criterion TBE-Urea Gel, 5%, 26-well, µl 1 gel 4 C 8 wk Criterion TBE-Urea Gel, %, 12+2 well, 45 µl 1 gel 4 C 8 wk Criterion TBE-Urea Gel, %, 18-well, 30 µl 1 gel 4 C 8 wk Criterion TBE-Urea Gel, %, 26-well, µl 1 gel 4 C 8 wk Criterion TBE-Urea Gel, %, 12+2 well, 45 µl 1 gel 4 C 8 wk Criterion TBE-Urea Gel, %, 18-well, 30 µl 1 gel 4 C 8 wk Criterion TBE-Urea Gel, %, 26-well, µl 1 gel 4 C 8 wk

11 Ready Gel Precast Gels Shelf Life Ready Gel Tris-HCl Gels Ready Gel Tris-HCl Gel, 7.5%, -well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, -well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 12%, -well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, -well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, -well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, -well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, -well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 7.5%, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 12%, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 7.5%, prep well, 4 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, prep well, 4 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 12%, prep well, 4 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, prep well, 4 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, prep well, 4 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, prep well, 4 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, prep well, 4 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 7.5%, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 12%, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 7.5%, 12-well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, 12-well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 12%, 12-well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, 12-well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, 12-well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, 12-well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, 12-well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 7.5%, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 12%, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, 9-well, 30 µl 1 gel 4 C 12 wk Shelf Life Ready Gel Tris-HCl Gel, 5%, -well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 5%, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 5%, prep well, 4 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 5%, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 5%, 12-well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 5%, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 18%, -well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 18%, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 18%, prep well, 4 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 18%, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 18%, 12-well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 18%, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 8 16%, -well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 8 16%, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 8 16%, prep well, 4 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 8 16%, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 8 16%, 12-well, µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 8 16%, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, 7 cm IPG well 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 12%, 7 cm IPG well 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, 7 cm IPG well 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 4 %, 7 cm IPG well 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, 8 16%, 7 cm IPG well 1 gel 4 C 12 wk Ready Gel Tris-HCl Gel, %, 7 cm IPG well 1 gel 4 C 12 wk Ready Gel Tris-Tricine Gels Ready Gel Tris-Tricine Gel, 16.5%, -well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-Tricine Gel, %, -well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-Tricine Gel, 16.5%, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-Tricine Gel, %, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-Tricine Gel, 16.5%, prep well, 4 µl 1 gel 4 C 12 wk Ready Gel Tris-Tricine Gel, %, prep well, 4 µl 1 gel 4 C 12 wk Ready Gel Tris-Tricine Gel, 16.5%, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-Tricine Gel, %, -well, µl 1 gel 4 C 12 wk Ready Gel Tris-Tricine Gel, 16.5%, 12-well, µl 1 gel 4 C 12 wk Ready Gel Tris-Tricine Gel, %, 12-well, µl 1 gel 4 C 12 wk Ready Gel Tris-Tricine Gel, 16.5%, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel Tris-Tricine Gel, %, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel TBE Gels Ready Gel TBE Gel, 5%, -well, 30 µl 1 gel 4 C 12 wk Ready Gel TBE Gel, %, -well, 30 µl 1 gel 4 C 12 wk Ready Gel TBE Gel, 5%, -well, µl 1 gel 4 C 12 wk (continues) Polyacrylamide IPG Strips and Precast Gels

12 Ready Gel Precast Gels (cont.) Shelf Life Ready Gel TBE Gels (cont.) Ready Gel TBE Gel, %, -well, µl 1 gel 4 C 12 wk Ready Gel TBE Gel, 5%, -well, µl 1 gel 4 C 12 wk Ready Gel TBE Gel, %, -well, µl 1 gel 4 C 12 wk Ready Gel TBE Gel, 5%, 12-well, µl 1 gel 4 C 12 wk Ready Gel TBE Gel, %, 12-well, µl 1 gel 4 C 12 wk Ready Gel TBE Gel, 5%, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel TBE Gel, %, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel TBE Gel, %, -well, 30 µl 1 gel 4 C 12 wk Ready Gel TBE Gel, %, -well, µl 1 gel 4 C 12 wk Ready Gel TBE Gel, %, -well, µl 1 gel 4 C 12 wk Ready Gel TBE Gel, %, 12-well, µl 1 gel 4 C 12 wk Ready Gel TBE Gel, %, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel TBE Gel, 4 %, -well, 30 µl 1 gel 4 C 12 wk Ready Gel TBE Gel, 4 %, -well, µl 1 gel 4 C 12 wk Ready Gel TBE Gel, 4 %, -well, µl 1 gel 4 C 12 wk Ready Gel TBE Gel, 4 %, 12-well, µl 1 gel 4 C 12 wk Ready Gel TBE Gel, 4 %, 9-well, 30 µl 1 gel 4 C 12 wk Ready Gel IEF Gels Ready Gel IEF Gel, ph 3, -well, 30 µl 1 gel 4 C 24 wk Ready Gel IEF Gel, ph 5 8, -well, 30 µl 1 gel 4 C 24 wk Ready Gel IEF Gel, ph 3, -well, µl 1 gel 4 C 24 wk Ready Gel IEF Gel, ph 5 8, -well, µl 1 gel 4 C 24 wk Ready Gel IEF Gel, ph 3, prep well, 4 µl 1 gel 4 C 24 wk Ready Gel IEF Gel, ph 5 8, prep well, 4 µl 1 gel 4 C 24 wk Ready Gel IEF Gel, ph 3, -well, µl 1 gel 4 C 24 wk Ready Gel IEF Gel, ph 5 8, -well, µl 1 gel 4 C 24 wk Ready Gel IEF Gel, ph 3, 12-well, µl 1 gel 4 C 24 wk Ready Gel IEF Gel, ph 5 8, 12-well, µl 1 gel 4 C 24 wk Ready Gel IEF Gel, ph 3, 9-well, 30 µl 1 gel 4 C 24 wk Ready Gel IEF Gel, ph 5 8, 9-well, 30 µl 1 gel 4 C 24 wk Ready Gel Zymogram Gels Ready Gel Zymogram Gel, %, gelatin, -well, 30 µl 1 gel 4 C 8 wk Ready Gel Zymogram Gel, 12%, casein, -well, 30 µl 1 gel 4 C 8 wk Ready Gel Zymogram Gel, %, gelatin, -well, µl 1 gel 4 C 8 wk Ready Gel Zymogram Gel, 12%, casein, -well, µl 1 gel 4 C 8 wk Ready Gel Zymogram Gel, %, gelatin, -well, µl 1 gel 4 C 8 wk Ready Gel Zymogram Gel, 12%, casein, -well, µl 1 gel 4 C 8 wk Ready Gel Zymogram Gel, %, gelatin, 12-well, µl 1 gel 4 C 8 wk Ready Gel Zymogram Gel, 12%, casein, 12-well, µl 1 gel 4 C 8 wk Shelf Life Ready Gel Zymogram Gel, %, gelatin, 9-well, 30 µl 1 gel 4 C 8 wk Ready Gel Zymogram Gel, 12%, casein, 9-well, 30 µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gels Ready Gel TBE-Urea Gel, 5%, -well, 30 µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, %, -well, 30 µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, %, -well, 30 µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, 5%, -well, µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, %, -well, µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, %, -well, µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, 5%, prep well, 4 µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, %, prep well, 4 µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, %, prep well, 4 µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, 5%, 12-well, µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, %, 12-well, µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, %, 12-well, µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, 5%, 9-well, 30 µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, %, 9-well, 30 µl 1 gel 4 C 8 wk Ready Gel TBE-Urea Gel, %, 9-well, 30 µl 1 gel 4 C 8 wk PROTEAN Precast Gels Shelf Life PROTEAN II Ready Gel Precast Gels PROTEAN II Ready Gel Tris-HCl Gel, %, 17 cm IPG well 1 gel 4 C 12 wk PROTEAN II Ready Gel Tris-HCl Gel, 12%, 17 cm IPG well 1 gel 4 C 12 wk PROTEAN II Ready Gel Tris-HCl Gel, %, 17 cm IPG well 1 gel 4 C 12 wk PROTEAN II Ready Gel Tris-HCl Gel, 8 16%, 17 cm IPG well 1 gel 4 C 12 wk PROTEAN II Ready Gel Tris-HCl Gel, %, -well 1 gel 4 C 12 wk PROTEAN II Ready Gel Tris-HCl Gel, 12%, -well 1 gel 4 C 12 wk PROTEAN II Ready Gel Tris-HCl Gel, %, -well 1 gel 4 C 12 wk PROTEAN II Ready Gel Tris-HCl Gel, 8 16%, -well 1 gel 4 C 12 wk PROTEAN II Ready Gel Tris-HCl Gel, %, -well 1 gel 4 C 12 wk PROTEAN II Ready Gel Tris-HCl Gel, 12%, -well 1 gel 4 C 12 wk PROTEAN II Ready Gel Tris-HCl Gel, %, -well 1 gel 4 C 12 wk PROTEAN II Ready Gel Tris-HCl Gel, 8 16%, -well 1 gel 4 C 12 wk PROTEAN Plus Gel PROTEAN Plus Tris-HCl Gel, 12%, 24 cm IPG well 1 gel 4 C 12 wk

13 DNA Standards Protein Standards bp Rulers EZ Load bp Molecular Ruler, 1,000 bp 0 µl 4 C bp Molecular Ruler, 1,000 bp 2 µl 4 C bp Rulers EZ Load bp Molecular Ruler, 1,000 bp 0 µl 4 C bp Molecular Ruler, 1,000 bp 2 µl 4 C EZ Load bp PCR Molecular Ruler, 3,000 bp 0 µl 4 C bp PCR Molecular Ruler, 3,000 bp 0 µl 4 C 0 bp Rulers EZ Load 0 bp Molecular Ruler, 0 8,000 bp 0 µl 4 C bp Molecular Ruler, 0 8,000 bp 0 µl 4 C 1 kb Rulers EZ Load 1 kb Molecular Ruler, 1 kb 0 µl 4 C kb Molecular Ruler, 1 kb 0 µl 4 C Mass Rulers EZ Load Precision Molecular Mass Ruler, ng 0 µl 4 C Precision Molecular Mass Ruler, ng 2 µl 4 C Other Molecular Rulers kb Molecular Ruler 400 µl 4 C AmpliSize Molecular Ruler, 2,000 bp 2 µl 4 C Pulsed Field Standards CHEF DNA Size Standard, 5 kb ladder µg 4 C CHEF DNA Size Standard, 8 48 kb ladder µg 4 C CHEF DNA Size Standard, lambda ladder 5 plugs 4 C CHEF DNA Size Marker, S. cerevisiae chromosomes 5 plugs 4 C CHEF DNA Size Marker, H. wingei chromosomes 5 plugs 4 C CHEF DNA Size Marker, S. pombe chromosomes 5 plugs 4 C Fluorescent Rulers bp Fluorescein-Labeled Ruler, 1,000 bp µl 4 C bp Texas Red-Labeled Ruler, 1,000 bp µl 4 C Molecular Markers EZ Load HT Molecular Marker, bp 2 kb 1.6 ml 4 C EZ Load HT Molecular Marker, 0 bp kb 1.6 ml 4 C Unstained Standards Polypeptide SDS-PAGE Standards, kd 0 µl C SDS-PAGE Standards, low range, kd 0 µl C SDS-PAGE Standards, high range, 45 0 kd 0 µl C SDS-PAGE Standards, broad range, kd 0 µl C Silver Stain SDS-PAGE Standards, low range, kd 0 µl C Silver Stain SDS-PAGE Standards, high range, 45 0 kd 0 µl C SDS-PAGE Standards, SYPRO Orange, broad range 0 µl C D SDS-PAGE Standards 0 µl C IEF Standards, pi range µl C Biotinylated SDS-PAGE Standards, low range 2 µl C Biotinylated SDS-PAGE Standards Kit, low range, avidin-ap 2 µl C Biotinylated SDS-PAGE Standards Kit, low range, avidin-hrp 2 µl C Biotinylated SDS-PAGE Standards, high range 2 µl C Biotinylated SDS-PAGE Standards Kit, high range, avidin-hrp 2 µl C Biotinylated SDS-PAGE Standards Kit, high range, avidin-ap 2 µl C Biotinylated SDS-PAGE Standards, broad range 2 µl C Biotinylated SDS-PAGE Standards Kit, broad range, avidin-hrp 2 µl C Biotinylated SDS-PAGE Standards Kit, broad range, avidin-ap 2 µl C Precision Plus Protein Unstained Standards 1 ml C Precision Plus Protein Standard Plugs, unstained 24 plugs 4 C Prestained Standards Prestained SDS-PAGE Standards, low range, 19 7 kd 0 µl C Prestained SDS-PAGE Standards, high range, 47 5 kd 0 µl C Prestained SDS-PAGE Standards, broad range, 7 5 kd 0 µl C Kaleidoscope Polypeptide Standards, kd 0 µl C Kaleidoscope Prestained Standards, kd 0 µl C Precision Plus Protein All Blue Standards, 2 kd 0 µl C Precision Plus Protein Dual Color Standards, 2 kd 0 µl C Precision Plus Protein Kaleidoscope Standards, 2 kd 0 µl C DNA and Protein Standards

14 Guide to Blot Detection Reagents and Sensitivities Gel electrophoresis of sample Transfer resolved proteins onto membrane Total protein detection Specific antigen detection Fluorescent blot stain Anionic dye Biotin-Blot kit Colloidal gold stain Block nonspecific sites SYPRO Ruby protein blot stain Amido Black or Coomassie Blue R ng 0 ng Color development with 4CN Optional gold enhancement ng pg 1 ng Incubate with specific antibody Incubate with detection reagent Chemiluminescence Color development HRP AP HRP AP Immun-Star HRP Immun-Star AP 1 3 pg pg Immun-Blot AP Immun-Blot Amplified AP pg pg Amplified Opti-4CN DAB Opti-4CN 4CN (Immun-Blot ) 5 pg pg pg 0 pg

15 Blotting Detection and Reagents Colorimetric Detection (Horseradish Peroxidase) Opti-4CN Substrate Kit 1 kit 4 C Opti-4CN Goat Anti-Rabbit Detection Kit 1 kit 4 C Opti-4CN Goat Anti-Mouse Detection Kit 1 kit 4 C Amplified Opti-4CN Substrate Kit 1 kit 4 C Amplified Opti-4CN Goat Anti-Rabbit Detection Kit 1 kit 4 C Amplified Opti-4CN Goat Anti-Mouse Detection Kit 1 kit 4 C Immun-Blot Assay Kit, Goat Anti-Rabbit IgG (H + L) HRP Conjugate 1 kit C Immun-Blot Assay Kit, Goat Anti-Mouse IgG (H + L) HRP Conjugate 1 kit C Immun-Blot Assay Kit, Goat Anti-Human IgG (H + L) HRP Conjugate 1 kit C Immun-Blot Assay Kit, Protein A-HRP Conjugate and 4CN 1 kit C Immun-Blot Assay Kit, Protein G-HRP Conjugate and 4CN 1 kit C HRP Color Development Reagent, 4CN 5 g C HRP Color Development Reagent, DAB 5 g C Colorimetric Detection (Alkaline Phosphatase) Immun-Blot Assay Kit, Goat Anti-Rabbit IgG (H + L) AP Conjugate 1 kit C Immun-Blot Assay Kit, Goat Anti-Mouse IgG (H + L) AP Conjugate 1 kit C Immun-Blot Assay Kit, Goat Anti-Human IgG (H + L) AP Conjugate 1 kit C Immun-Blot Assay Kit, Goat-Anti Rabbit IgG (H + L)-Biotin 1 kit C Blotting Grade Biotinylated Alkaline Phosphatase 1 ml C Blotting Grade Streptavidin 1.0 mg C AP Color Development Reagent, BCIP 300 mg C AP Color Development Reagent, NBT 600 mg C Chemiluminescent Detection (Horseradish Peroxidase) Immun-Star HRP Substrate 0 ml 4 C Immun-Star HRP Substrate ml 4 C Immun-Star Goat-Anti Rabbit (GAR)-HRP Detection Reagents 0 ml 4 C Immun-Star Goat-Anti Mouse (GAM)-HRP Detection Reagents 0 ml 4 C Immun-Star Goat-Anti Mouse (GAM)-HRP Detection Kit 1 kit 4 C Immun-Star Goat-Anti Rabbit (GAR)-HRP Detection Kit 1 kit 4 C Immun-Star Goat-Anti Rabbit (GAR)-HRP Conjugate 2 ml 4 C Immun-Star Goat-Anti Mouse (GAM)-HRP Conjugate 2 ml 4 C Chemiluminescent Detection (Alkaline Phosphatase) 170- Immun-Star Goat-Anti Mouse (GAM)-AP Detection Kit 1 kit 4 C Immun-Star Goat-Anti Rabbit (GAR)-AP Detection Kit 1 kit 4 C Immun-Star AP Substrate Pack 1 pack 4 C Immun-Star Goat Anti-Mouse (GAM)-AP Intro Kit 1 kit 4 C Immun-Star Goat Anti-Rabbit (GAR)-AP Intro Kit 1 kit 4 C Immun-Star AP Substrate 1 ml 4 C 170- Blotting Reagents Pack 1 pack 4 C Antibody and Substrate Conjugates Horseradish Peroxidase Conjugate Substrate Kit 1 kit C Goat Anti-Rabbit IgG-HRP (H + L) 2 ml C Goat Anti-Mouse IgG-HRP (H + L) 2 ml C 172- Goat Anti-Human IgG-HRP (H + L) 2 ml C Protein A-HRP Conjugate 1 ml C Protein G-HRP Conjugate 1 ml C Avidin-HRP Conjugate 2 ml C Alkaline Phosphatase Conjugate Substrate Kit 1 kit C Goat Anti-Rabbit IgG-AP (H + L) 1 ml C Goat Anti-Mouse IgG-AP (H + L) 1 ml C Goat Anti-Human IgG-AP (H + L) 1 ml C Biotinylated Goat Anti-Rabbit IgG (H + L) 1 ml C Avidin-AP 1 ml C Streptavidin-AP 0.5 ml C NHS-Biotin 4 ml C Blotting Grade Biotinylated AP 1 ml C Precision Protein StrepTactin-HRP Conjugate 0.3 ml 4 C Precision Protein StrepTactin-AP Conjugate 0.3 ml 4 C Blotting Reagents and Buffers Blot Processing Buffer, x TBS 1 L Ambient x PBS 1 L Ambient % Tween 1 L Ambient Tween, EIA grade ml Ambient x PBS/1% Casein 1 L 4 C x TBS/1% Casein 1 L 4 C Blotting Grade Blocker, nonfat dry milk 300 g Ambient Gelatin, EIA grade 0 g Ambient Mini Trans-Blot Filter Paper, thick, 7.5 x cm sheets Ambient Extra-Thick Blot Paper, 7.5 x cm 60 sheets Ambient Blot Stains Enhanced Colloidal Gold Total Protein Detection Kit 1 kit 4 C Colloidal Gold Total Protein Stain 0 ml 4 C SYPRO Ruby Protein Blot Stain 0 ml Ambient Background Remover for X-Ray Film 170- De-Expose Background Remover Solution, x 2 ml 4 C Blotting Membranes and Reagents

16 Blotting Membranes Nitrocellulose Nitrocellulose Membrane, 0.2 µm, 7 x 8.4 cm sheets Ambient Nitrocellulose Membrane, 0.2 µm, 13.5 x 16.5 cm sheets Ambient Nitrocellulose Membrane, 0.2 µm, x cm 5 sheets Ambient Nitrocellulose Membrane, 0.2 µm, 30 cm x 3.5 m 1 roll Ambient Nitrocellulose Membrane, 0.2 µm, 8.5 x 13.5 cm sheets Ambient Nitrocellulose Membrane, 0.2 µm, 26.5 x 28 cm sheets Ambient Nitrocellulose Membrane, 0.45 µm, 7 x 8.4 cm sheets Ambient Nitrocellulose Membrane, 0.45 µm, 9 x 12 cm sheets Ambient Nitrocellulose Membrane, 0.45 µm, x 9.2 cm sheets Ambient Nitrocellulose Membrane, 0.45 µm, x cm sheets Ambient Nitrocellulose Membrane, 0.45 µm, 11.5 x 16 cm sheets Ambient Nitrocellulose Membrane, 0.45 µm, x cm 5 sheets Ambient Nitrocellulose Membrane, 0.45 µm, 30 cm x 3.5 m 1 roll Ambient Nitrocellulose Membrane, 0.45 µm, 8.5 x 13.5 cm sheets Ambient Nitrocellulose Membrane, 0.45 µm, 26.5 x 28 cm sheets Ambient Nitrocellulose Membrane Sandwiches Nitrocellulose/Filter Paper Sandwich, 0.2 µm, 8.5 x 13.5 cm pack Ambient Nitrocellulose/Filter Paper Sandwich, 0.2 µm, 8.5 x 13.5 cm pack Ambient Nitrocellulose/Filter Paper Sandwich, 0.2 µm, 7 x 8.5 cm pack Ambient Nitrocellulose/Filter Paper Sandwich, 0.2 µm, 7 x 8.5 cm pack Ambient Nitrocellulose/Filter Paper Sandwich, 0.45 µm, 8.5 x 13.5 cm pack Ambient Nitrocellulose/Filter Paper Sandwich, 0.45 µm, 8.5 x 13.5 cm pack Ambient Nitrocellulose/Filter Paper Sandwich, 0.45 µm, 7 x 8.5 cm pack Ambient Nitrocellulose/Filter Paper Sandwich, 0.45 µm, 7 x 8.5 cm pack Ambient Supported Nitrocellulose Supported Nitrocellulose Membrane, 0.2 µm, 7 x 8.4 cm sheets Ambient Supported Nitrocellulose Membrane, 0.2 µm, 30 cm x 3 m 1 roll Ambient Supported Nitrocellulose Membrane, 0.2 µm, 8.5 x 13.5 cm sheets Ambient Supported Nitrocellulose Membrane, 0.2 µm, 26.5 x 28 cm sheets Ambient Supported Nitrocellulose Membrane, 0.45 µm, 7 x 8.4 cm sheets Ambient Supported Nitrocellulose Membrane, 0.45 µm, 30 cm x 3 m 1 roll Ambient Supported Nitrocellulose Membrane, 0.45 µm, 8.5 x 13.5 cm sheets Ambient Supported Nitrocellulose Membrane, 0.45 µm, 26.5 x 28 cm sheets Ambient PVDF Membranes Immun-Blot PVDF Membrane, 7 x 8.4 cm sheets Ambient Immun-Blot PVDF Membrane, x cm sheets Ambient Immun-Blot PVDF Membrane, x cm sheets Ambient Immun-Blot PVDF Membrane, 26 cm x 3.3 m 1 roll Ambient Sequi-Blot PVDF Membrane, 7 x 8.4 cm sheets Ambient Sequi-Blot PVDF Membrane, x cm sheets Ambient Sequi-Blot PVDF Membrane, x cm sheets Ambient Sequi-Blot PVDF Membrane, x cm sheets Ambient Sequi-Blot PVDF Membrane, x cm 3 sheets Ambient Sequi-Blot PVDF Membrane, 26 cm x 3.3 m 1 roll Ambient Immun-Blot PVDF Membrane, x 28 cm sheets Ambient Sequi-Blot PVDF Membrane, x 28 cm sheets Ambient PVDF Membrane Sandwiches Immun-Blot PVDF/Filter Paper Sandwich, 8.5 x 13.5 cm pack Ambient Immun-Blot PVDF/Filter Paper Sandwich, 8.5 x 13.5 cm pack Ambient Sequi-Blot PVDF/Filter Paper Sandwich, 8.5 x 13.5 cm pack Ambient Sequi-Blot PVDF/Filter Paper Sandwich, 8.5 x 13.5 cm pack Ambient Immun-Blot PVDF/Filter Paper Sandwich, 7 x 8.5 cm pack Ambient Immun-Blot PVDF/Filter Paper Sandwich, 7 x 8.5 cm pack Ambient Sequi-Blot PVDF/Filter Paper Sandwich, 7 x 8.5 cm pack Ambient Sequi-Blot PVDF/Filter Paper Sandwich, 7 x 8.5 cm pack Ambient Zeta-Probe Membranes Zeta-Probe Membrane, 9 x 12 cm sheets Ambient Zeta-Probe Membrane, x cm sheets Ambient Zeta-Probe Membrane, x cm sheets Ambient Zeta-Probe Membrane, x cm sheets Ambient Zeta-Probe Membrane, x cm sheets Ambient Zeta-Probe Membrane, x cm 3 sheets Ambient Zeta-Probe Membrane, cm x 3.3 m 1 roll Ambient Zeta-Probe Membrane, 30 cm x 3.3 m 1 roll Ambient Zeta-Probe Membrane, 7 x cm sheets Ambient Zeta-Probe GT Membranes Zeta-Probe GT Membrane, 9 x 12 cm sheets Ambient Zeta-Probe GT Membrane, x cm sheets Ambient Zeta-Probe GT Membrane, x cm sheets Ambient Zeta-Probe GT Membrane, x cm sheets Ambient Zeta-Probe GT Membrane, x cm sheets Ambient Zeta-Probe GT Membrane, x cm 3 sheets Ambient Zeta-Probe GT Membrane, cm x 3.3 m 1 roll Ambient Zeta-Probe GT Membrane, 30 cm x 3.3 m 1 roll Ambient Zeta-Probe GT Membrane, 7 x cm sheets Ambient

17 Gel Reagents, Stains, and Buffers Gel-Casting Reagents Acrylamide, 99.9% 0 g Ambient Acrylamide/Bis, 19:1 1 g 4 C Acrylamide/Bis, 29:1 1 g Ambient Acrylamide/Bis,.5:1 1 g Ambient Bis Crosslinker g Ambient % Acrylamide/Bis Solution, 19:1 0 ml 4 C % Acrylamide/Bis Solution, 29:1 0 ml 4 C % Acrylamide/Bis Solution,.5:1 0 ml 4 C % Acrylamide/Bis Solution, 19:1 0 ml 4 C % Acrylamide/Bis Solution, 29:1 0 ml 4 C % Acrylamide/Bis Solution,.5:1 0 ml 4 C % Acrylamide Solution 0 ml 4 C % Bis Solution 0 ml 4 C SDS 1 kg Ambient TEMED ml Ambient Dithiothreitol 5 g Ambient Ammonium Persulfate g Ambient Mercaptoethanol ml Ambient Tricine g Ambient Glycine 1 kg Ambient Tris 1 kg Ambient Sucrose 1 kg Ambient EDTA 0 g Ambient Urea 1 kg Ambient Boric Acid 1 kg Ambient Gel Stains Bio-Safe Coomassie Stain 1 L Ambient Coomassie Brilliant Blue R-2 Staining Solutions Kit 1 kit Ambient Zinc Stain and Destain Kit 1 kit Ambient Silver Stain Plus Kit 1 kit Ambient Silver Stain Kit 1 kit 4 C SYPRO Orange Fluorescent Protein Stain 0 µl 4 C SYPRO Ruby Protein Gel Stain 1 L Ambient Radiant Red Fluorescent RNA Stain ml 4 C Copper Stain and Destain Kit 1 kit Ambient Ethidium Bromide Solution ml Ambient Ethidium Bromide Tablets tablets Ambient Dodeca Silver Stain Kit, large L Ambient Dodeca Silver Stain Kit, small 7 L Ambient Premixed Buffers Resolving Gel Buffer, 1.5 M Tris-HCl, ph L Ambient Stacking Gel Buffer, 0.5 M Tris-HCl, ph L Ambient x Tris/Glycine/SDS 1 L Ambient x Tris/Glycine/SDS 5 L cube Ambient x Tris/Glycine 1 L Ambient x Tris/Glycine 5 L cube Ambient x Tris/Boric Acid/EDTA 1 L Ambient x Tris/Boric Acid/EDTA 5 L cube Ambient x Tris/Boric Acid/EDTA, extended range 1 L Ambient x Tris/Tricine/SDS 1 L Ambient x Tris/Acetic Acid/EDTA 1 L Ambient x Tris/Acetic Acid/EDTA 5 L cube Ambient x Tris/CAPS 1 L Ambient x SSC 5 L cube Ambient x SSC 1 L Ambient Laemmli Sample Buffer 30 ml Ambient Native Sample Buffer 30 ml 4 C Tricine Sample Buffer 30 ml Ambient TBE/Urea Sample Buffer 30 ml 4 C Nucleic Acid Sample Buffer, 5x ml Ambient Zymogram Sample Buffer 30 ml Ambient Zymogram Renaturation Buffer 1 ml Ambient Zymogram Development Buffer 1 ml Ambient SDS Solution, % (w/v) 2 ml Ambient SDS Solution, % (w/v) 1 L Ambient IEF Sample Buffer 30 ml Ambient x IEF Anode Buffer 2 ml Ambient x IEF Cathode Buffer 2 ml Ambient Criterion XT Buffers and Reagents XT MOPS Running Buffer, x 0 ml Ambient XT MES Running Buffer, x 0 ml Ambient XT Tricine Running Buffer, x 0 ml Ambient XT Sample Buffer, 4x ml Ambient XT Reducing Agent, x 1 ml Ambient XT MOPS Buffer Kit 1 kit Ambient XT MES Buffer Kit 1 kit Ambient XT Tricine Buffer Kit 1 kit Ambient Gel Reagents, Stains, and Buffers

18 Sample Preparation Chart Nucleic Acids Proteins Isolation Cleanup Extraction/Fractionation Cleanup Plasmid Dye Terminator Removal Total Protein Salt Removal Aurum plasmid kits Quantum Prep plasmid kits Freeze N Squeeze columns Total RNA Aurum total RNA kits AquaPure RNA kits Genomic DNA AquaPure genomic DNA kit Chelex resins, molecular biology grade SEQueaky Kleen H 2 O kits SEQueaky Kleen H 2 O adaptors Micro Bio-Spin 30 columns Buffer Exchange/ Probe Cleanup Micro Bio-Spin 6 columns Bio-Spin 6 columns Econo-Pac DG cartridge Micro Bio-Spin 30 columns Bio-Spin 30 columns PCR Cleanup PCR Kleen Columns ReadyPrep protein extraction kit (total protein) Solubility/Hydrophobicity ReadyPrep sequential extraction kit ReadyPrep protein extraction kit (soluble/insoluble) Econo-Pac Macro-Prep methyl HIC support/cartridge Econo-Pac Macro-Prep t-butyl HIC support/cartridge Protein Size Bio-Gel P-2 resin Bio-Gel P-4 resin Bio-Gel P-6 resin Bio-Gel P- resin Cellular Location Bio-Gel P-30 resin Bio-Gel P-60 resin Bio-Gel P- resin ReadyPrep protein extraction kit (cytoplasmic/nuclear) ReadyPrep protein extraction kit (membrane I or membrane II) ReadyPrep protein extraction kit (signal) ReadyPrep 2-D cleanup kit Micro Bio-Spin 6 columns Bio-Spin 6 columns Econo-Pac DG cartridge Albumin/IgG Removal Aurum serum protein kit Aurum Affi-Gel Blue columns Econo-Pac Affi-Gel Blue cartridge Econo-Pac protein A columns/cartridges Econo-Pac DEAE Affi-Gel Blue columns/cartridges Ionic Contaminant Removal (Detergent, Lipid, and Phenolic Compounds) ReadyPrep 2-D cleanup kit Protein Charge Disulfide Bond Removal Macro-Prep High Q resin Macro-Prep High S resin Macro-Prep DEAE resin Macro-Prep CM resin Aurum AEX kit Aurum CEX kit ReadyPrep reduction-alkylation kit

19 Protein Sample Preparation Protein Assays ReadyPrep Kits ReadyPrep 2-D Cleanup Kit 1 kit Ambient ReadyPrep Sequential Extraction Kit 1 kit Ambient ReadyPrep Reduction-Alkylation Kit 1 kit Ambient ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) 1 kit Ambient ReadyPrep Protein Extraction Kit (Membrane I) 1 kit Ambient ReadyPrep Protein Extraction Kit (Signal) 1 kit Ambient ReadyPrep Protein Extraction Kit (Total Protein) 1 kit Ambient ReadyPrep Protein Extraction Kit (Soluble/Insoluble) 1 kit Ambient ReadyPrep Protein Extraction Kit (Membrane II) 1 kit Ambient ReadyPrep Components and Related Products ReadyPrep Sequential Extraction Kit Reagent 1 1 vial Ambient ReadyPrep Sequential Extraction Kit Reagent 2 1 vial Ambient ReadyPrep Sequential Extraction Kit Reagent 3 1 vial Ambient ReadyPrep 2-D Starter Kit 1 kit 4 C ReadyPrep 2-D Starter Kit Rehydration/Sample Buffer ml Ambient ReadyPrep 2-D Starter Kit Equilibration Buffer I, with DTT ml Ambient ReadyPrep 2-D Starter Kit Equilibration Buffer II, without DTT ml Ambient E. coli Protein Sample, lyophilized 2.7 mg 4 C ReadyPrep Overlay Agarose ml 4 C PROTEAN Plus Overlay Agarose 1 ml 4 C ReadyPrep Proteomic Grade Water 0 ml Ambient ReadyPrep 2-D Rehydration/Sample Buffer 1 ml Ambient Column-Based Purification Aurum Serum Protein Mini Kit 2 preps 4 C Aurum Serum Protein Mini Kit preps 4 C Aurum Affi-Gel Blue, Mini Kit 2 pack 4 C Aurum Affi-Gel Blue, Mini Columns pack 4 C Aurum Affi-Gel Blue, Mini Columns pack 4 C Econo-Pac Protein A Columns 5 columns 4 C 732- Econo-Pac Protein A Kit 1 kit 4 C Econo-Pac Serum IgG Purification Kit 1 kit 4 C Aurum CEX Mini Kit 2 pack 4 C Aurum CEX Mini Kit pack 4 C Aurum CEX Mini Columns pack 4 C Aurum CEX Mini Columns pack 4 C Aurum AEX Mini Kit 2 pack 4 C Aurum AEX Mini Kit pack 4 C Aurum AEX Mini Columns pack 4 C Aurum AEX Mini Columns pack 4 C Protein Purification Sampler Pack V 1 pack 4 C Protein Purification Sampler Pack I 1 pack 4 C Micro Bio-Spin and Bio-Spin Columns Refer to Prepacked Spin Columns for Sample Cleanup in the Nucleic Acid Sample Preparation section. Size/ Storage Protein Assays and Cuvettes 0-01 Quick Start Bradford Protein Assay Kit 1, 1 kit 4 C bovine serum albumin standard 0-02 Quick Start Bradford Protein Assay Kit 2, 1 kit 4 C bovine serum albumin standard set 0-03 Quick Start Bradford Protein Assay Kit 3, 1 kit 4 C bovine -globulin standard 0-04 Quick Start Bradford Protein Assay Kit 4, 1 kit 4 C bovine -globulin standard set 0-05 Quick Start Bradford 1x Dye Reagent 1 L 4 C 0-06 Quick Start Bovine Serum Albumin Standard 2 mg/ml 4 C 0-07 Quick Start Bovine Serum Albumin Standard Set 7 standards 4 C 0-08 Quick Start Bovine -Globulin Standard 2 mg/ml 4 C 0-09 Quick Start Bovine -Globulin Standard Set 7 standards 4 C Bio-Rad Protein Assay Kit I, bovine -globulin standard 1 kit 4 C Bio-Rad Protein Assay Kit II, bovine serum albumin standard 1 kit 4 C Bio-Rad Protein Assay Dye Reagent Concentrate 4 ml 4 C DC Protein Assay Kit I, bovine -globulin standard 1 kit 4 C DC Protein Assay Kit II, bovine serum albumin standard 1 kit 4 C DC Protein Assay Reagents Package 1 package 4 C RC DC Protein Assay Kit I, bovine -globulin standard 1 kit 4 C RC DC Protein Assay Kit II, bovine serum albumin standard 1 kit 4 C Protein Standard I, bovine -globulin 1 bottle 4 C Protein Standard II, bovine serum albumin 1 bottle 4 C Standard Disposable Polystyrene Cuvettes, 3.5 ml cuvettes Ambient Semimicrovolume Disposable Polystyrene Cuvettes, 1.5 ml cuvettes Ambient Sample Preparation and Protein Assays

20 Nucleic Acid Sample Preparation Size/ Storage Products for Nucleic Acid Isolation Quantum Prep Plasmid Miniprep Kit 1 kit Ambient Quantum Prep Midiprep Kit 1 kit Ambient Quantum Prep Maxiprep Kit 1 kit Ambient Freeze N Squeeze Spin Columns columns Ambient Freeze N Squeeze Spin Columns columns Ambient InstaGene Matrix ml 4 C Aurum Total RNA Mini Kit preps Ambient Aurum Total RNA 96 Kit 2 x 96-well preps Ambient AquaPure RNA Isolation Kit preps Ambient AquaPure RNA Blood Kit preps Ambient Master Blaster RNA Extraction Reagent preps Ambient AquaPure Genomic DNA Isolation Kit preps Ambient AquaPure Genomic DNA Tissue Kit preps Ambient AquaPure Genomic DNA Blood Kit preps Ambient Aurum Vacuum Manifold 1 manifold Ambient Aurum Plasmid Mini Kit preps Ambient Aurum Plasmid Midi Kit preps Ambient Aurum Plasmid Maxi Kit preps Ambient Aurum Plasmid 96 Kit 2 x 96-well preps Ambient Products for Sequencing Cleanup SEQueaky Kleen H 2 O Dye Terminator Removal Kit 2 x 96-well, 4 C 4 collection plates SEQueaky Kleen H 2 O Bulk Kit x 96-well, 4 C collection plates SEQueaky Kleen H 2 O Adaptors Set of 2 Ambient SEQueaky Kleen H 2 O Starter Kit, for half-skirt plates 1 kit Ambient SEQueaky Kleen H 2 O Starter Kit, for unskirted plates 1 kit Ambient SEQueaky Kleen H 2 O Starter Kit, for full-skirt plates 1 kit Ambient SEQueaky Kleen H 2 O Starter Kit, for segmented plates 1 kit Ambient SEQueaky Kleen H 2 O Refill Kit, for half-skirt plates 1 kit Ambient SEQueaky Kleen H 2 O Refill Kit, for unskirted plates 1 kit Ambient SEQueaky Kleen H 2 O Refill Kit, for full-skirt plates 1 kit Ambient SEQueaky Kleen H 2 O Refill Kit, for segmented plates 1 kit Ambient SEQueaky Kleen H 2 O Half-Skirt Plates pack Ambient SEQueaky Kleen H 2 O Unskirted Plates pack Ambient SEQueaky Kleen H 2 O Full-Skirt Plates pack Ambient SEQueaky Kleen H 2 O Segmented Plates pack Ambient Size/ Storage PCR Kleen Spin Columns PCR Kleen Spin Columns columns Ambient PCR Kleen Spin Columns columns Ambient Prepacked Spin Columns for Sample Cleanup* Micro Bio-Spin 6 Columns, in Tris buffer columns 4 C Micro Bio-Spin 6 Columns, in Tris buffer columns 4 C Micro Bio-Spin 6 Columns, in Tris buffer 1,000 columns 4 C Micro Bio-Spin 6 Columns, in SSC buffer columns 4 C Micro Bio-Spin 6 Columns, in SSC buffer columns 4 C Micro Bio-Spin 6 Columns, in SSC buffer 1,000 columns 4 C Micro Bio-Spin 30 Columns, in Tris buffer columns 4 C Micro Bio-Spin 30 Columns, in Tris buffer columns 4 C Micro Bio-Spin 30 Columns, in Tris buffer 1,000 columns 4 C Micro Bio-Spin 30 Columns, in Tris buffer, RNase-free columns 4 C Micro Bio-Spin 30 Columns, in Tris buffer, RNase-free columns 4 C Micro Bio-Spin 30 Columns, in SSC buffer columns 4 C Micro Bio-Spin 30 Columns, in SSC buffer columns 4 C Micro Bio-Spin 30 Columns, in SSC buffer 1,000 columns 4 C Bio-Spin 6 Columns, in Tris buffer columns 4 C Bio-Spin 6 Columns, in Tris buffer columns 4 C Bio-Spin 6 Columns, in Tris buffer 1,000 columns 4 C Bio-Spin 30 Columns, in Tris buffer columns 4 C Bio-Spin 30 Columns, in Tris buffer columns 4 C Bio-Spin 30 Columns, in Tris buffer 1,000 columns 4 C Bio-Spin 6 Columns, in SSC buffer columns 4 C Bio-Spin 30 Columns, in SSC buffer columns 4 C 732- Econo-Pac DG Desalting Columns 30 columns Ambient * Bio-Spin and Micro Bio-Spin columns can also be used for protein sample preparation.

21 Agarose Powders and Agarose Precast Gels Agarose Powder Certified Molecular Biology Agarose 1 g Ambient Certified PCR Agarose 1 g Ambient Certified Low Range Ultra Agarose g Ambient Certified Megabase Agarose 1 g Ambient Certified Low-Melt Agarose 1 g Ambient Certified PCR Low-Melt Agarose 1 g Ambient Mini ReadyAgarose Gels, TBE ReadyAgarose 0.8% TBE Gel, 8-well 1 gel 4 22 C ReadyAgarose 0.8% TBE Gel, 8-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 1.0% TBE Gel, 8-well 1 gel 4 22 C ReadyAgarose 1.0% TBE Gel, 8-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 3.0% TBE Gel, 8-well 1 gel 4 22 C ReadyAgarose 3.0% TBE Gel, 8-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 0.8% TBE Gel, 12-well 1 gel 4 22 C ReadyAgarose 0.8% TBE Gel, 12-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 1.0% TBE Gel, 12-well 1 gel 4 22 C ReadyAgarose 1.0% TBE Gel, 12-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 3.0% TBE Gel, 12-well 1 gel 4 22 C ReadyAgarose 3.0% TBE Gel, 12-well, with ethidium bromide 1 gel 4 22 C Mini ReadyAgarose Gels, TAE ReadyAgarose 0.8% TAE Gel, 8-well 1 gel 4 22 C ReadyAgarose 0.8% TAE Gel, 8-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 1.0% TAE Gel, 8-well 1 gel 4 22 C ReadyAgarose 1.0% TAE Gel, 8-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 3.0% TAE Gel, 8-well 1 gel 4 22 C ReadyAgarose 3.0% TAE Gel, 8-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 0.8% TAE Gel, 12-well 1 gel 4 22 C ReadyAgarose 0.8% TAE Gel, 12-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 1.0% TAE Gel, 12-well 1 gel 4 22 C ReadyAgarose 1.0% TAE Gel, 12-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 3.0% TAE Gel, 12-well 1 gel 4 22 C ReadyAgarose 3.0% TAE Gel, 12-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 1.0% TAE Gel, 2 x 8-well 1 gel 4 22 C Wide Mini ReadyAgarose Gels, TBE ReadyAgarose 0.8% TBE Gel, -well 1 gel 4 22 C ReadyAgarose 0.8% TBE Gel, -well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 1.0% TBE Gel, -well 1 gel 4 22 C ReadyAgarose 1.0% TBE Gel, -well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 3.0% TBE Gel, -well 1 gel 4 22 C ReadyAgarose 3.0% TBE Gel, -well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 0.8% TBE Gel, 32-well 1 gel 4 22 C ReadyAgarose 0.8% TBE Gel, 32-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 1.0% TBE Gel, 32-well 1 gel 4 22 C ReadyAgarose 1.0% TBE Gel, 32-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 3.0% TBE Gel, 32-well 1 gel 4 22 C ReadyAgarose 3.0% TBE Gel, 32-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 1.0% TBE Gel, 2 x 32-well 1 gel 4 22 C ReadyAgarose 1.0% TBE Gel, 2 x 32-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 3.0% TBE Gel, 2 x 32-well 1 gel 4 22 C ReadyAgarose 3.0% TBE Gel, 2 x 32-well, with ethidium bromide 1 gel 4 22 C Wide Mini ReadyAgarose Gels, TAE ReadyAgarose 0.8% TAE Gel, -well 1 gel 4 22 C ReadyAgarose 0.8% TAE Gel, -well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 1.0% TAE Gel, -well 1 gel 4 22 C ReadyAgarose 1.0% TAE Gel, -well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 3.0% TAE Gel, -well 1 gel 4 22 C ReadyAgarose 3.0% TAE Gel, -well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 0.8% TAE Gel, 32-well 1 gel 4 22 C ReadyAgarose 0.8% TAE Gel, 32-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 1.0% TAE Gel, 32-well 1 gel 4 22 C ReadyAgarose 1.0% TAE Gel, 32-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 3.0% TAE Gel, 32-well 1 gel 4 22 C ReadyAgarose 3.0% TAE Gel, 32-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 1.0% TAE Gel, 2 x 32-well 1 gel 4 22 C ReadyAgarose 1.0% TAE Gel, 2 x 32-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 3.0% TAE Gel, 2 x 32-well 1 gel 4 22 C ReadyAgarose 3.0% TAE Gel, 2 x 32-well, with ethidium bromide 1 gel 4 22 C ReadyAgarose 96 Plus Gels, TBE ReadyAgarose 96 Plus 1% TBE Gel, with ethidium bromide 1 gel 4 22 C ReadyAgarose 96 Plus 3% TBE Gel, with ethidium bromide 1 gel 4 22 C ReadyAgarose 96 Plus Gels, TAE ReadyAgarose 96 Plus 1% TAE Gel, with ethidium bromide 1 gel 4 22 C ReadyAgarose 96 Plus 3% TAE Gel, with ethidium bromide 1 gel 4 22 C Agarose Powders and Precast Gels

22 Gene Transfer Amplification Size/ Storage Electro-Competent Cells EP-Max B Electro-Competent Cells 5 x 0. ml 70 C EP-MaxB F' Electro-Competent Cells 5 x 0. ml 70 C EP-MaxB T1-Resistant Electro-Competent Cells 5 x 0. ml 70 C Chemi-Competent Cells C-Max 5 Chemi-Competent Cells 5 x 0. ml 70 C C-Max5 Chemi-Competent Cells x 0.05 ml 70 C C-Max5 F' Chemi-Competent Cells 5 x 0. ml 70 C C-Max5 F' Chemi-Competent Cells x 0.05 ml 70 C Electroporation Cuvettes Gene Pulser /MicroPulser Cuvettes, 0.1 cm gap 5 cuvettes Ambient Gene Pulser/MicroPulser Cuvettes, 0.1 cm gap cuvettes Ambient Gene Pulser/MicroPulser Cuvettes, 0.1 cm gap 0 cuvettes Ambient Gene Pulser/MicroPulser Cuvettes, 0.2 cm gap 5 cuvettes Ambient Gene Pulser/MicroPulser Cuvettes, 0.2 cm gap cuvettes Ambient Gene Pulser/MicroPulser Cuvettes, 0.2 cm gap 0 cuvettes Ambient Gene Pulser/MicroPulser Cuvettes, 0.4 cm gap 5 cuvettes Ambient Gene Pulser/MicroPulser Cuvettes, 0.4 cm gap cuvettes Ambient Gene Pulser/MicroPulser Cuvettes, 0.4 cm gap 0 cuvettes Ambient Lipofection TransFectin Lipid Reagent 0.5 ml 4 C TransFectin Lipid Reagent 1.0 ml 4 C TransFectin Lipid Reagent 5 x 1.0 ml 4 C Fluorescent Assay Kits DNA Quantitation Kit (Hoechst) 1 kit 4 C FluorAce -Galactosidase Reporter Assay Kit 1 kit 4 C FluorAce -Glucuronidase Reporter Assay Kit 1 kit 4 C Size/ Storage Reagents itaq DNA Polymerase 2 units C itaq DNA Polymerase 5,000 units C MgCl 2 Solution, mm 1. ml Ambient dntp Mix, mm each dntp 0 ml C iq Supermix reactions C iq Supermix 0 reactions C iq Supermix 1,000 reactions C iq SYBR Green Supermix reactions C iq SYBR Green Supermix 0 reactions C iq SYBR Green Supermix 1,000 reactions C itaq SYBR Green Supermix With ROX 0 reactions C itaq SYBR Green Supermix With ROX 0 reactions C itaq SYBR Green Supermix With ROX 1,000 reactions C iscript cdna Synthesis Kit reactions C iscript cdna Synthesis Kit reactions C iscript One-Step RT-PCR Kit With SYBR Green reactions C iscript One-Step RT-PCR Kit With SYBR Green 0 reactions C iscript One-Step RT-PCR Kit for Probes reactions C iscript One-Step RT-PCR Kit for Probes 0 reactions C Micro Test Tubes and Plates Well 0.2 ml Thin-Wall PCR Plates plates Ambient Well 0.2 ml PCR Plate Mats 5 mats Ambient Well 0.2 ml PCR Plate Caps 300 strips Ambient Optical-Quality Sealing Tape sheets Ambient ml Thin-Wall Tubes, natural 1,000 tubes Ambient ml Thin-Wall PCR 8-Tube Strips, natural 1 strips Ambient ml Thin-Wall PCR 8-Cap Strips, natural 1 strips Ambient ml Thin-Wall Tubes, natural 1,000 tubes Ambient ml Thin-Wall PCR 12-Tube Strips, natural 80 strips Ambient ml Thin-Wall PCR 12-Cap Strips, natural 80 strips Ambient Well PCR Plates plates Ambient Well PCR Plate Mats mats Ambient

23 Competent Cell Product Guide Application Helpful Mutation/Marker Benefit Host Cell Genomic library construction mrr-hsdrms-mcrbc Allows cloning of methylated DNA EP-Max tona Confers resistance to bacteriophage infection EP-Max T1-Resistant cdna library construction tona Confers resistance to bacteriophage infection EP-Max Plasmid rescue/propagation reca Reduced risk of recombination events EP-Max enda Higher-quality, higher-copy plasmid preps EP-Max/C-Max ssdna synthesis F' episome Allows infection by bacteriophage M13 EP-Max F', C-Max F' Selection for transformants Tn Tetracycline resistance (allows selection of F' hosts) EP-Max F', C-Max F' laczm Allows blue/white screening by -complementation EP-Max, C-Max Phage display F' episome, supe Required for growth of amber mutants C-Max F' phoa insertions, phoa phoa Absence of alkaline phosphatase activity C-Max fusion analysis Expression of toxic proteins laci q gene Permits regulated expression from lac, tac, EP-Max F', C-Max F' and trc promoters Gene Transfer and Amplification

24 Place Magnet here Trademarks Bio-Rad Representative Business Card Affi-Gel, Affi-Prep, AmpliSize, AquaPure, Aurum, Bio-Gel, Bio-Lyte, Bio-Safe, Bio-Scale, Bio-Sil, Bio-Silect, Bio-Spin, Biotin-Blot, Certified, Chelex, CHT, C-Max, Criterion, Criterion XT, DC, De-Expose, Dodeca, Econo-Column, Econo-Pac, EP-Max, EZ Load, EZ Load HT, FluorAce, Freeze N Squeeze, Gene Pulser, Immun-Blot, Immun-Star, InstaGene, iq, iscript, itaq, Kaleidoscope, Macro-Prep, MAPS, Master Blaster, Micro Bio-Spin, MicroPulser, Mini Trans-Blot, Opti-4CN, PCR Kleen, Poly-Prep, Precision Plus Protein, PROTEAN, PROTEAN Plus, Quantum Prep, Quick Start, Radiant, RC DC, ReadyAgarose, ReadyAgarose 96 Plus, Ready Gel, ReadyPrep, ReadyStrip, SEQueaky Kleen, Sequi-Blot, Silver Stain Plus, TransFectin, UNO, UNOsphere, Zeta-Probe, and Zeta-Probe GT are trademarks or registered trademarks of Bio-Rad Laboratories, Inc. Bio-Rad Laboratories, Inc. is licensed by Molecular Probes, Inc. to sell SYPRO products for research use only, under US patent 5,616,2. Bio-Rad Laboratories, Inc. is licensed by Molecular Probes, Inc. to sell reagents containing SYBR Green I for use in real-time PCR, for research purposes only. Coomassie is a trademark of BASF Aktiengesellschaft. PEEK is a trademark of Victrex PLC. StrepTactin is a trademark of Institut für Bioanalytik GmbH. SYBR, SYPRO, and Texas Red are trademarks of Molecular Probes, Inc. Tween is a trademark of ICI Americas, Inc. Bio-Rad Laboratories, Inc. Life Science Group Web site USA (800) 4BIORAD Australia Austria (01) Belgium Brazil Canada (905) China (86-21) Czech Republic Denmark Finland France Germany Hong Kong Hungary India (91-124) /113/114, 64092/93 Israel Italy Japan Korea Latin America Mexico The Netherlands New Zealand Norway Poland Portugal Russia Singapore South Africa Spain Sweden Switzerland Taiwan (8862) / United Kingdom Bulletin 2367 US/EG Rev C Sig 13

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