from different sources High Yields Exceptional Purity Incredible Value

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1 Thermo Scientific GeneJET Nucleic Acid Purification Kits high yields of pure & RNA from different sources High Yields Exceptional Purity Incredible Value

2 Reliable and RNA Purification The Thermo Scientific GeneJET product line contains essential nucleic acid purification tools to support and facilitate your experimental workflow. With more than 3 years of experience in developing and manufacturing molecular biology products, we see a growing demand for simple and reliable tools for and RNA sample preparation. The GeneJET TM and RNA purification kits address the need for high-purity nucleic acids ready to use in downstream applications and offer detailed protocols for routine and difficult sample types. Trusted silica membrane technology in the form of a convenient spin column for purification of and RNA Isolate nucleic acids with superior purity and yields compared to other kits on the market Purified nucleic acids are ready to use in common downstream applications GeneJET kits have been extensively tested on a wide variety of sample types and include specialized protocols Product Selection Guide Nucleic Acid Purification Plasmid Genomic Total RNA Fragments Miniprep Midiprep Maxiprep Plant Blood from Cells & Tissues Plant RNA Blood RNA RNA from Cells & Tissues From PCR From Gels page 3 page 4 page 5 page 6 For additional details visit

3 GeneJET Genomic Purification Kits Efficient genomic purification from different sources. GeneJET Genomic Purification Kit Purify RNA-free g from a wide variety of tissues or cells in only 2 minutes GeneJET Whole Blood Genomic Purification Mini Kit Rapidly purify RNA-free g from whole blood and body fluids with no separate cell lysis step GeneJET Plant Genomic Purification Mini Kit Purify without clogging-prone lysate filtration step Material-specific protocols provided Recover g greater than 3 kb in size, including nuclear, mitochondrial and chloroplast Spin-columns based on silica membrane technology Isolated can be used directly in PCR, qpcr, and other common enzymatic reactions Specifications for GeneJET Genomic Purification Kits Genomic Purification Cells & tissues Whole Blood Plant Amount of sample material Varies 2 µl mg Yield Varies Up to 1 µg Up to 32 µg Purity A26/ Column binding capacity µg µg µg Isolate genomic from a variety of samples: Mammalian cells Mammalian tissues: Muscle Heart Liver Whole blood Fresh or frozen Anticoagulated blood Buffy coat Bone marrow Body fluids (urine) Plants Multiple plant species Various plant tissues (leaves, roots, seed, needles) Bacteria Yeasts Filamentous fungi Procedural time 2 min 2 min 3 min Format and protocol Spin columns, capped, preassembled with collection tubes and individually packed. Centrifugation or vacuum protocol. High yields of genomic purified from various sources with the GeneJET Genomic Purification Kit Genomic, µg rat liver 1 mg rat spleen 5 mg mouse heart 1 mg HeLa 2x1 8 cells Thermo Scientific A B C E. coli mammalian blood 2x1 9 cells 2 µl Yields of genomic purified using the GeneJET Genomic Purification Kit and kits from other s. Plant genomic purified from various sources with the GeneJET Plant Genomic Purification Mini Kit M M Genomic from different plant material samples purified using the GeneJET Plant Genomic Purification Mini Kit. 1- Arabidopsis, 2- Tobacco leaves, 3- Tomato leaves, 4- Spinach leaves, 5- Wheat leaves, 6- Grape leaves, 7- Onion leaves, 8- Rice leaves, 9- Corn seeds, 1- Sunflower stalk, 11- Corn roots, 12- Beef roots, 13- Cucumber fruit, 14 - Sugar cane. GeneJET Genomic Purification Kit GeneJET Whole Blood Genomic Purification Mini Kit GeneJET Plant Genomic Purification Mini Kit K721 K722 K781 K782 K791 K

4 GeneJET Plasmid Purification Kits Rapid isolation of microgram to milligram quantities of highquality plasmid. GeneJET Plasmid Miniprep Kit Small scale isolation of up to 2 µg of high quality plasmid in 14 min GeneJET Plasmid Midiprep Kit Medium scale isolation of up to 2 µg of high quality plasmid in 6 min GeneJET Plasmid Maxiprep Kit Large scale isolation of up to 7 µg of high quality plasmid in 6 min Silica-based membrane technology - save time and improve yields compared to gravity flow columns. Consistent and Reproducible Miniprep Yields M A pbluescript-based high copy number plasmid was purified from E.coli culture with 6 different GeneJET Spin Columns M - FastRuler High Range Ladder plasmid isolated with 6 different GeneJET Spin Columns Isolated plasmid can be used directly in FastDigest or conventional restriction digestion, sequencing, PCR, in vitro transcription and transfection of robust cell lines. Relaxed Supercoiled Specifications for GeneJET Plasmid Purification Kits Plasmid Purification Kits Mini Midi Maxi Bacterial culture volume 5 ml ml 2 ml Yield 2 µg 2 µg 7 µg concentration 4 ng/µl 4 ng/µl 4 ng/µl Purity A26/ Column binding capacity 2 µg 4 µg µg Midi and Maxi spin columns offer considerably faster procedure and higher yields compared to gravity flow anion exchange columns Procedural time 14 min 6 min 6 min Format and protocol types Spin columns, capped, preassembled with collection tubes. Centrifugation or vacuum protocol. High yields and high concentration plasmid purified with the GeneJET Midiprep Kit., µg Thermo Scientific A B C 1st elution 2nd elution D, ng/µg Thermo Scientific 1st elution 2nd elution A B C D Yields of high copy plasmid obtained using GeneJET plasmid midiprep kit and other kits for midscale plasmid purification at maximal recommended bacterial cell culture volumes. Concentrations of eluted obtained after the purification of high copy plasmid using different kits for midscale plasmid purification at maximal recommended bacterial cell culture volumes. GeneJET Plasmid Miniprep Kit GeneJET Plasmid Midiprep Kit GeneJET Plasmid Maxiprep Kit K2 K3 K481 K482 K491 K

5 GeneJET Total RNA Purification Kits High purity total RNA isolation from different sources. Isolate RNA from a variety of samples: Mammalian cells Mammalian tissues: Muscle Heart Liver Whole blood Anticoagulated blood Buffy coat Bone marrow Plants Multiple plant species Various plant tissues (leaves, roots, seed, needles) Pathogens (virus) Bacteria Yeast Filamentous fungi GeneJET RNA Purification Kit Purify total RNA from a wide variety of sources GeneJET Whole Blood RNA Purification Mini Kit Purify RNA from whole blood and body fluids with no DNase step GeneJET Plant RNA Purification Mini Kit Purify RNA from various plants and plant tissues with no DNase step Spin-columns based on silica membrane technology Specialized and detailed protocols for different sample types Purified RNA can be used in a wide range of downstream applications Specifications for GeneJET RNA Purification Kits RNA Purification Kits Cells & tissues Whole Blood Plants Amount of sample material Varies µl mg Yield Up to 7 µg Up to 5 µg Up to 7 µg Purity A26/ Column binding capacity > µg > µg > µg Procedural time 15 min 15 min 2 min Format and protocol types Spin columns, capped, preassembled with collection tubes and individually packed. Centrifugation protocol. Total RNA purified from various sources with the GeneJET RNA Purification Kits. M Total RNA purified from mg of tomato leaves using the GeneJET Plant RNA Purification Kit and kits from other s. M- RiboRuler High Range RNA Ladder. 1- Thermo Scientific, 2-5 other s. M Total RNA purified from various sources using the GeneJET RNA Purification Kit. M- RiboRuler High Range RNA Ladder (#SM1821) 1- mouse liver, 2- mouse spleen, 3- HeLa cells, 4- Cos-7 cells, 5- Saccharomyces cerevisiae, 6- Escherichia coli DH5α GeneJET RNA Purification Kit K731 K732 GeneJET Whole Blood RNA Purification Mini Kit K761 GeneJET Plant RNA Purification Mini Kit K81 K82 2 2

6 GeneJET PCR Purification and Gel Extraction Kits Effective fragment recovery from reaction mixtures or agarose gels. GeneJET PCR Purification Kit recovery up to % (25 bp 2 kb) Effective removal of primers, unincorporated nucleotides, enzymes and salts from PCR and other enzymatic reaction mixtures GeneJET Gel Extraction Kit recovery up to 95% (25 bp 2 kb) Purify fragments from standard or low-melt agarose gels run with TAE or TBE buffer. Spin-columns based on silica membrane technology can be used in common downstream applications such as sequencing, restriction digestion, ligation, cloning and in vitro transcription Superior purification of fragments from PCR reaction mixtures Recovery rate, % bp bp 1 kb Size 5.5 kb 1 kb Thermo Scientific A B Specifications for GeneJET PCR Purification and Gel Extraction Kits Kit types PCR Purification Gel Extraction Amount of sample material.8 ml 1 g agarose gel Recovery rate 9-% 7-95% fragment range 25 bp-2 kb 25 bp-2 kb Purity A26/ Column binding capacity 25 µg 25 µg Procedural time 5 min 15 min Format and protocol types Spin columns, capped, preassembled with collection tubes. Centrifugation or vacuum protocol. GeneJET PCR Purification Kit GeneJET Gel Extraction Kit K71 K72 K691 K For further information visit Ordering Information: North America 26 Crescent Dr., Suite Lafayette, CO 826 Toll free: Fax: Europe Opelstraße St. Leon-Rot, Germany Tel: +49 () Fax: +49 () Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. US Literature # 12411J1U Euro Literature # PB_211_186