2) Mechanism of separation of contaminants present in air by fibrous media is

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1 X Courses» Industrial Biotechnology Announcements Course Forum Progress Mentor Unit 6 - Week 5 Course outline How to access the portal Week 1 Week 2 Week 3 Week 4 Week 5 Assignment 1 1) Air can be sterilized by Heat UV ray. Week 5 Lecture 21: Upstream Air sterilizer Lecture 22: Upstream Medium sterilizer Lecture 23: Upstream Medium sterilizer (continued) Lecture 24: Downstream solid-liquid separators Lecture 25: Downstream evaporator, crystallizer Lecture 26: Downstream liquid-liquid extraction, distillation, chromatography Week 5 : Lecture Material 2) Mechanism of separation of contaminants present in air by fibrous media is Interception Inertial impaction Diffusion 3) The interception efficiency can be expressed as (where b is the width of the upstream air flow and d p is the fiber diameter) b/d p b d p d p /b b d p /100 b/d p 4) In a fermentation process,air sterilizer is used to reduce to 10microorganisms. What will be the filter efficiency? 1 in million 1 in 10 million 1/6

2 Quiz : Week 5 Assignment 1 Feedback for Week 5 Week 5 Assignment 1 Solution Week 6 Week 7 Week 8 Week 9 1 in 100 million 1 in 1000 million 1 in 10 million 5) Steam sterilization of the fermentation medium is usually carried out in the range of Week 10 Week 11 Week ) Which sterilization method does not deal with the destruction of the cells Chemical method method Radiation method Thermal method method 7) The decimal reduction time (D) can be expressed as (where k d is thermal death rate constant) D = k d D = 2.303/k d D = k d /2.303 None of the above D = 2.303/k d 8) The pore size of the filter to remove bacteria, yeasts, and fungi is in the range of 2-5 μm 1-2 μm μm None of the above μm 9) The continuous medium sterilization includes Heating period Holding period Cooling period 2/6

3 10) The X 90 value for air sterilization is The time required to reduce microbial population by 90% The time required to traverse survivor curve by one log cycle The depth of the filter required to reduce population by 90% The depth of the filter required to reduce population by 10% The depth of the filter required to reduce population by 90% 11) A pipe has a diameter of 0.2 m in which a fluid flows with a velocity of 0.3 m/s. Determine the Reynolds number. Assume kinematic viscosity = m 2 /s. (kinematic viscosity is the ratio of dynamic viscosity to the density of the substance) ) Which of the following dimensionless numberhas significant role in continuous medium sterilization? Mach number Peclet number Prandtl number Grashof number Peclet number 13) Ethanol is separated from the fermentation broth by the process of Sublimation Evaporation 14) Rotary vacuum filter is usually used for the separation of Bacteria Yeast Mold Virus 3/6

4 Mold 15) Depth filtration involves Straining Impingement Entanglement Entanglement 16) Mechanism involved in plate and frame filter press is Surface filtration Depth filtration a and b both None of the above Surface filtration 17) Chemical pretreatment process for the separation solids from the liquid requires Bentonite clay Isinglass Kieselguhr Alum Alum 18) Major drawbacks of the ultrafiltration process are Pressure drop Life of the membrane Fouling All the above All the above 19) Crystallization does not involve Agitation Cooling Sublimation Salting out Sublimation 20) What is the driving force played important role in natural circulation evaporator? External force 4/6

5 Velocity gradient Viscosity gradient Density gradient Density gradient 21) Separation of the chemicals with the help of relative solubility of two different immiscible liquids is known as Liquid-liquid extraction Centrifugation Liquid-liquid extraction 22) Miscible liquids with different boiling point can be separated by Crystallization 23) Which separation technique is based on mobile and stationary phases? Sedimentation 24) Quantitative analysis of volatile components present in the sample is carried out by GC with the help of Chromatogram Chromatograph Chromatophore Graph Chromatogram Previous Page End 5/6

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