Dried Blood Spot Analysis From The Clinic To The Laboratory
|
|
- Karin Harrell
- 5 years ago
- Views:
Transcription
1 Dried Blood Spot Analysis From The Clinic To The Laboratory Joanne Mather, Pharmaceutical Life Sciences Waters Corporation 211 Waters Corporation 1
2 From The Clinic To The Lab Blood Spot Sampling First Used In Neonatal Screening 211 Waters Corporation 2
3 Dried Blood Spots Timeline of Implementation >1 yrs ago DBS were exclusively used for neonatal, infectious disease screening, therapeutic drug monitoring Dried Blood Spots have been around for over 4 years 5 Years Ago 2 manuscripts DBS to discovery-stage PK and metabolite ID (Merck Frost) Present Day Potential application to routine drug development was realized within GlaxoSmithKline in 26 Quickly saw the broadly applicable advantages offered by DBS Other pharmaceutical companies (sanofi-aventis et. al.) becoming heavily involved with DBS (as well as CROs and vendors) 211 Waters Corporation 3
4 On Board DBS Uptake To date GSK has developed >15 validated DBS methods for nearly 75 compounds >2 studies supported using DBS >1 compounds have reached phase 1 pre-clin GLP studies 211 Waters Corporation 4
5 Issues and Advantages of Dried Blood Spot Analysis [Card based sample collection] Reduced animal usage/ cost/ 3 R s Lower shipping costs Reduced compound needed for tox studies Improved data- PK data from toxicology animals [Small sample volumes 1-2µL] Assay sensitivity [Background card interference] More complicated methods development [Resolution from metabolites and matrix needed] High LC separation efficiency 211 Waters Corporation 5
6 Analytical Issues Non liquid format Current work flow will not work with this format Current approach requires punching, either manual or automated Card background may cause issues Matrix effects from blood components Resolution from metabolites and matrix needed for quality 211 Waters Corporation 6
7 Analytical Issues Non liquid format Current work flow will not work with this format Current approach require punching, either manual or automated Card background may cause issues Matrix effects from blood components 211 Waters Corporation 7
8 Blood Spotting Aliquot 15μL blood per spot 3 spots per sample plus spare Using a pipette or capillary o Do NOT allow tip to touch card surface! Dry for 2 hours at room temperature Ship & store in sealable bags containing desiccant 211 Waters Corporation 8
9 Sample Prep & Analysis Analytical sample obtained by punching small circular disc (typically 3 mm) from centre of DBS Manually Automation BSD1 Extract disc in organic solvent (typically methanol) containing internal standard Quantification by validated LC- MS/MS assay 211 Waters Corporation 9
10 Chromatography Requirements Long separation Short Separations 211 Waters Corporation 1
11 % % % % Analysis with LC/MS/MS Quantification by validated LC-MS/MS assay 1721_JoM_DBS_ : MS2 ES+ TIC 1.5e _JoM_DBS_138 1: MRM of 3 Channels ES > 297 (Alprazolam Metabolite) 2.55e _JoM_DBS_138 1: MRM of 3 Channels ES > (AlprazolamD5) 4.15e _JoM_DBS_138 1: MRM of 3 Channels ES > 281 (Alprazolam) 5.2e7 Use of UPLC: Increased sensitivity Increased resolution Increased speed of analysis Time 211 Waters Corporation 11
12 % % % % % Rapid Mode Switching Precursor Ion Confirmation Scan 1721_JoM_DBS_ : MS2 ES+ TIC 1.5e _JoM_DBS_138 1: MRM of 3 Channels ES > 297 (Alprazolam Metabolite) 2.55e7 PIC Scan Acquisition 1721_JoM_DBS_4 3 (.79) e _JoM_DBS_138 1: MRM of 3 Channels ES > (AlprazolamD5) 4.15e _JoM_DBS_138 1: MRM of 3 Channels ES > 281 (Alprazolam) 5.2e Time Confirm peak identity in the presence of a complex matrix in one analytical run. m/z 211 Waters Corporation 12
13 Analytical Issues Non liquid format Current work flow will not work with this format Current approach require punching, either manual or automated Card background may cause issues Matrix effects from blood components 211 Waters Corporation 13
14 Dried blood spot microvolume sampling for DMPK Three types are available (Whatman, GE), which have different chemistries. FTA DMPK-A (treated) FTA DMPK-B (treated) FTA DMPK-C (untreated) FTA DMPK A & B cards contain proprietary chemical mixtures that lyse cells, inactivate pathogens and denature degradative enzymes and other proteins. FTA DMPK-C cards contain only cellulose Waters Corporation 14
15 Rapid MS to MRM Switching 211 Waters Corporation 15
16 % % % Using RADAR to Evaluate Card Type To Be Used 121_JoM_DBS_cards_13 Full scan blank card -background signal : MS2 ES+ TIC 5.98e DMPK A _JoM_DBS_cards_17 2: MS2 ES TIC e DMPK B _JoM_DBS_cards_25 2: MS2 ES+ 1 TIC 5.98e DMPK C Waters Corporation 16 Time
17 % % % Using RADAR to Evaluate Card Type To Be Used Analyte Response 221_JoM_DBS_cards_ : MRM of 3 Channels ES > 281 (Alprazolam) 9.61e5 DMPK A _JoM_DBS_cards_2 1: MRM of 3 Channels ES > 281 (Alprazolam) 9.61e5 1.9 DMPK B _JoM_DBS_cards_33 1: MRM of 3 Channels ES > 281 (Alprazolam) 9.61e5 DMPK C Waters Corporation 17 Time
18 Analytical Issues Non liquid format Current work flow will not work with this format Current approach require punching, either manual or automated Card background may cause issues Matrix effects from blood components 211 Waters Corporation 18
19 Matrix The Challenge Matrix effects from blood components Gain adequate resolution from matrix interferences and other analytes/ metabolites Maximize sensitivity Maintain or improve productivity Removal of matrix components 211 Waters Corporation 19
20 % % % % RADAR - Monitor matrix complexity Gradient A Gradient B Full Scan 1721_JoM_DBS_ : MS2 ES+ 2.1 TIC 1.54e _JoM_DBS_ : MS2 ES+ TIC 1.5e _JoM_DBS_135 1: MRM of 3 Channels ES > 281 (Alprazolam) 6.36e _JoM_DBS_138 1: MRM of 3 Channels ES > 281 (Alprazolam) 5.2e7 MRM Resolved more effectively from the endogenous blood and card matrix peaks Time Time 211 Waters Corporation 2
21 Alternate Approach for Calculating Matrix Factors LC Column MRM Signal 1) Determine Analyte Profile AN and IS injected through the column 2) Inject Solvent Blank 3) Inject Extracted Matrix Blank Solvent Blank injected with post column infusion of AN and IS Extracted Blank injected with post column infusion of AN and IS Analyte = AN Internal Standard = IS AN and IS infused post-column 211 Waters Corporation 21
22 Automating Matrix Factor Calculations: Integrated Intellistart Fluidics 211 Waters Corporation 22
23 MassLynx TargetLynx Simplifies Calculating The Matrix Effects TargetLynx automatically calculates matrix effects from 6 injections Results Presented in Final Report 211 Waters Corporation 23
24 Removal of Phospholipids 211 Waters Corporation 24
25 Removal of Phospholipids 211 Waters Corporation 25
26 Removal of Phospholipids 211 Waters Corporation 26
27 Response % % Sensitivity and Robustness Compound name: Alprazolam Correlation coefficient: r =.99968, r^2 = Calibration curve: * x Response type: Internal Std ( Ref 2 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/x, Axis trans: None.55.5 Alprazolam (benzodiazepine) 1pg/mL - 5ng/mL 421_JoM_DBS_QC_216 1 Alprazolam 1pg/mL 1.47 MRM of 3 Channels ES > 281 (Alprazolam) 2.57e _JoM_DBS_QC_31 MRM of 3 Channels ES+ 1 TIC 2.57e Blank Time ng/ml Concentration (ng/ml) Average Std Dev QC QC QC QC QC Published- Journal Bioanalysis Feb 211, Vol 3:4 p Rapid Analysis of DBS samples with sub 2µm LC/MS/MS Mather J et al. 211 Waters Corporation 27
28 % Response Compound name: Sitamaquine Correlation coefficient: r =.99991, r^2 = From Dried Blood Spot Calibration curve: * x Response type: External Std, Area Curve type: Linear, Origin: Exclude, Weighting: Null, Axis trans: None LLOQ of 5pg/mL with a linear dynamic range of 4 orders of magnitude. Sitamaquine (WR-626) is an orally active 8- aminoquinoline analog for the potential treatment of visceral leishmaniasis (also known as kala-azar, black fever, and Dumdum fever). ng/ml FEB21_PR_ MRM of 2 Channels ES > (Sitamaquine) 5.44e3 LLOQ of 5pg/mL Time Waters Corporation 28
29 Heart Cut Configuration 2D UPLC/MS/MS Trapping Decrease Matrix Effects and Increase Sensitivity At-Column Dilution Inject Large Volume Samples in Strong Solvent No evaporation & reconstitution 211 Waters Corporation 29
30 2 Dimensional Dried Blood Spot Analysis of Rosuvastatin Direct injection of methanol extract from DBS sample 8uL injection of methanol At column dilution No At Column Dilution With At Column Dilution 211 Waters Corporation 3
31 2 Dimensional UPLC/MS/MS Bioanalysis 1pg/mL Blank 211 Waters Corporation 31
32 Conclusions Dried Blood Spots offer a significant scientific and expense advantage. The lower sample volume derived from the dried blood spots and the background card matrix poses a significant analytical challenge. Information rich LC/MS/MS (RADAR) can simplify the process of DBS analysis/ method development. Analysis times as low as 3 minutes possible. 2D LC to remove Matrix components (trap) and allow injection of large volume samples in high organic solvent. 211 Waters Corporation 32
33 Acknowledgements Paul Rainville Jennifer Simeone Robert Plumb David Little Jessalynn Wheaton Erin Chambers Christopher Evans GSK Neil Spooner - GSK Ian Wilson - Astra Zeneca 211 Waters Corporation 33
Waters Solutions for DMPK and Biomarker Analysis. Waters User Meeting ASMS 2011 Denver, Colorado. Stephen McDonald
Waters Solutions for DMPK and Biomarker Analysis Waters User Meeting ASMS 2011 Denver, Colorado Stephen McDonald 2011 Waters Corporation 1 Drug Development Process Pre-clinical testing R&D - 18 months
More information6 applications that will make a difference in your bioanalytical lab
[ 6 applications that will make ] a difference in your bioanalytical lab Streamline method development Increase sensitivity in negative ion mode Enhance full-scan sensitivity in metabolite detection Quantify
More informationSimultaneous in vivo Quantification and Metabolite Identification of Plasma Samples Using High Resolution QTof and Routine MS E Data Analysis
Simultaneous in vivo Quantification and Metabolite Identification of Plasma Samples Using High Resolution QTof and Routine MS E Data Analysis Mark Wrona, 1 Paul Rainville, 1 Eric Langlois, 2 Nigel Ewing,
More informationInvestigation of Solid Phase Microextraction as an Alternative to Dried Blood Spot
Investigation of Solid Phase Microextraction as an Alternative to Dried Blood Spot Craig Aurand and Robert Shirey Supelco, Div. of Sigma-Aldrich Bellefonte, PA 16823 USA T414068 sigma-aldrich.com/analytical
More informationExploring Extra Sensitivity Using ionkey/ms with the Xevo G2-XS Q-Tof HRMS for Small Molecule Pharmaceutical Analysis in Human Plasma
Exploring Extra Sensitivity Using ionkey/ms with the Xevo G2-XS Q-Tof HRMS for Small Molecule Pharmaceutical Analysis in Human Plasma Yun Wang Alelyunas, Mark D. Wrona, Jim Murphy, Angela Doneanu, Gregory
More informationPractical Aspects of Dried Blood Spot (DBS) Bioanalytical Assay Development and Application. Qin C. Ji CASSS, 09 September 2010
Practical Aspects of Dried Blood Spot (DBS) Bioanalytical Assay Development and Application Qin C. Ji CASSS, 09 September 2010 1 Outline Introduction Potential advantages of DBS DBS assay development Result
More informationCAMAG DBS-MS 500 DRIED BLOOD SPOT EXTRACTION SYSTEM FOR LC-MS
CAMAG DBS-MS 500 DRIED BLOOD SPOT EXTRACTION SYSTEM FOR LC-MS Direct Analysis FULLY AUTOMATED DBS EXTRACTION SYSTEM FOR MASS SPECTROMETRY Dried blood spot sampling was introduced around 40 years ago. It
More informationA Novel Dried Matrix Microsampling. Eliminates the Volume Based Hematocrit Bias Associated with DBS Sub-punch Workflows
A Novel Dried Matrix Microsampling Device that Eliminates the Volume Based Hematocrit Bias Associated with DBS Sub-punch Workflows Stuart Kushon1; Allen Bischofberger1; Anna Carpenter1; Philip Denniff2;
More informationeasypunch STARlet: Increasing throughput and lowering sample volume in DMPK studies by automating Whatman FTA TM DMPK DBS card processing
easypunch STARlet: Increasing throughput and lowering sample volume in DMPK studies by automating Whatman FTA TM DMPK DBS card processing Application note Top 3 reasons for automation of this assay FTA
More informationAutomatic Combination of Orthogonal Separation Modes for Multiple Components in Mixtures
Automatic Combination of Orthogonal Separation Modes for Multiple Components in Mixtures Thomas E. Wheat, Amanda B. Dlugasch, and Patricia R. McConville Waters Corporation IFPAC January 26, 2016 2016 Waters
More informationTabisam Khan 1, David R. Little 1, Liyu Yang 2, Jiwen Chen 2, Parya Nouri 2, Samy Tadros 2, Patrick J. Rudewicz 2
AIMS High throughput bioanalysis plays a central role in the drug discovery and development process. The resulting data are used to determine a drug candidate's pharmacokinetic parameters that are crucial
More informationDRUG DISCOVERY & 2 September 2010
LC-MS/MS QUANTITATION IN DRUG DISCOVERY & DEVELOPMENT Ludmila Alexandrova 2 September 2010 1 For personal use only. Please do not reuse or reproduce without the author s permission. 2 Presentation Outline
More informationQuantitatitive Analysis of Phosphorothioate Oligonucleotide in Human Plasma Using LC-MS/MS with On-Line Extraction
Laixin Wang, Sherry Liu, Qiuying Zhu, Scott Reuschel and Min Meng Tandem Labs Quantitatitive Analysis of Phosphorothioate Oligonucleotide in Human Plasma Using LC-MS/MS with On-Line Extraction Introduction
More informationA Rapid and Reproducible Immuno-MS Platform from Sample Collection to Quantitation of IgG
PO-CON1457E A Rapid and Reproducible Immuno-MS Platform from Sample Collection to Quantitation of IgG ASMS 14 ThP766 Rachel Lieberman 1, David Colquhoun 1, Jeremy Post 1, Brian Feild 1, Scott Kuzdzal 1,
More informationIncreasing Throughput and Efficiency with Exactive LC/MS and Triple Quadrupole LC/MS/MS
Increasing Throughput and Efficiency with Exactive LC/MS and Triple Quadrupole LC/MS/MS Nicholas Duczak Thermo Scientific Annual Mass Spectrometry Users Meeting Somerset, NJ October 12th, 2011 Lead Finding
More information[ APPLICATION NOTE ] Determination of Acidic Herbicides in Water Using Liquid Chromatography-Tandem Quadrupole Mass Spectrometry APPLICATION BENEFITS
Deteration of Acidic Herbicides in Water Using Liquid Chromatography-Tandem Quadrupole Mass Spectrometry Renata Jandova, 1 Simon Hird, 1 Euan Ross, 1 and Marijn Van Hulle 2 1 Waters Corporation, Wilmslow,
More informationA Rapid imethod TM Test for Analysis of Four Immunosuppressants
imethod Test A Rapid imethod TM Test for Analysis of Four Immunosuppressants The following description outlines the instrument requirements and expected results obtainable from the Spark Holland imethod
More informationBenefits of 2D-LC/MS/MS in Pharmaceutical Bioanalytics
Application Note Small Molecule Pharmaceuticals Benefits of 2D-LC/MS/MS in Pharmaceutical Bioanalytics Avoiding Matrix Effects Increasing Detection Sensitivity Authors Jonas Dinser Daiichi-Sankyo Europe
More informationUPLC/MS/MS Method for the Quantification of Trastuzumab in Human Serum at the 5-nM Level Using Xevo TQD MS and ACQUITY UPLC H-Class System
UPLC/MS/MS Method for the Quantification of Trastuzumab in Human Serum at the 5-nM Level Using Xevo TQD MS and ACQUITY UPLC H-Class System Catalin Doneanu and Robert Plumb Waters Corporation, Milford,
More informationSmall molecule bioanalytical method development and transfer: is a plug and play approach possible?
Small molecule bioanalytical method development and transfer: is a plug and play approach possible? Luca Ferrari Clinical Pharmacology and Bioanalytical R&D, pred Pharmaceutical Sciences, Roche Innovation
More informationQuantitative Analysis of pesticides including Phenoxy-acids in surface and drinking water matrices using UPLC-ON-LINE-SPE-MS/MS
Quantitative Analysis of pesticides including Phenoxy-acids in surface and drinking water matrices using UPLC-ON-LINE-SPE-MS/MS Alexandre Paccou Demo Centers and Application Support Manager, Southern Europe
More informationImproved SPE for UPLC/MS Determination of Diquat and Paraquat in Environmental
Improved SPE for UPLC/MS Determination of Diquat and Paraquat in Environmental Samples Michael S.Young, Jeremy C. Shia, Kim vantran, Kevin M. Jenkins and Masayo Yabo Waters Corporation 34 Maple Street,
More informationIllicit Drug Analysis in Urine Using 2D LC-MS/MS for Forensic Toxicology
Claude Mallet 1 and Sabra Botch-Jones 2 1 Waters Corporation, Milford, MA, USA 2 Boston University School of Medicine, Boston, MA, USA APPLICATION BENEFITS Fast extraction protocol (2 min) 1 ppt detection
More informationPharmaceutical LC/MS Solutions from Agilent Technologies
Pharmaceutical LC/MS Solutions from Agilent Technologies Application Compendium Overview LC/MS plays a key role in the drug discovery and drug development process. Since the introduction of electrospray
More informationHigh throughput analysis at microscale: performance of ionkey/ms with Xevo G2-XS QTof under rapid gradient conditions
JOURNAL OF APPLIED BIOANALYSIS, October 2015, p. 128-135. http://dx.doi.org/10.17145/jab.15.021 (ISSN 2405-710X) Vol. 1, No. 4 APPLICATION NOTE High throughput analysis at microscale: performance of ionkey/ms
More informationHigh throughput analysis at microscale: performance of ionkey/ms with Xevo G2-XS QTof under rapid gradient conditions
JOURNAL OF APPLIED BIOANALYSIS, October 2015, p. 128-135. http://dx.doi.org/10.17145/jab.15.021 (ISSN 2405-710X) Vol. 1, No. 4 APPLICATION NOTE High throughput analysis at microscale: performance of ionkey/ms
More informationFeedback From the 2011 APQ Open Forum. DBS and Microsampling: Moving Past the Hype to Knowledge and Implementation
Feedback From the 2011 APQ Open Forum DBS and Microsampling: Moving Past the Hype to Knowledge and Implementation Eric N. Fluhler, Pfizer, on behalf of AAPS Bioanalytical Focus Group DBS and Microsampling
More informationWaters QUATTRO PREMIER XE
Www.IetLtd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment Waters QUATTRO PREMIER XE Looking for a more intelligent way to overcome these
More informationBackground. Ferring Pharmaceuticals
Background Ferring Pharmaceuticals Founded in 195 and have since then worked with peptides Today, focus are on peptides as well as proteins Many of our peptides are agonists targeting the oxytocin or vasopressin
More informationExtraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE
Application Note AN880 Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE EXPRESS ABN Prior to LC-MS/MS Analysis Page 1 Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE EXPRESS ABN Prior
More informationMoving steroid analyses from immunoassay to LC-MS/MS. Arieh Cohen
Moving steroid analyses from immunoassay to LC-MS/MS challenges and pitfalls Arieh Cohen Neonatal screening and ormone analysis Dept. Of Clinical Biochemistry Statens Serum Institut aco@ssi.dk Background
More informationWorld-leading LC/MS/MS technology for quantitation and identification API LC/MS/MS System
World-leading LC/MS/MS technology for quantitation and identification API 3000 LC/MS/MS System rel Accelerate your drug development cycle The API 3000 LC/MS/MS System leads the field in sensitivity, specificity,
More informationMicroflow LC-MS with the turn of a key
[ ionkey/ms ] Microflow LC-MS with the turn of a key Dramatically enhance your sensitivity for both quantitative and qualitative chromatographic data, with an integrated microflow LC-MS solution that is
More informationAutomation for Improving the Workflows for LC-MS/MS. Francois Espourteille, Ph.D. Manager, Applications
Automation for Improving the Workflows for LC-MS/MS Francois Espourteille, Ph.D. Manager, Applications Discussion Overview Challenges of sample preparation in LC-MS analysis TurboFlow Technology Multiplexing
More informationCLSI C60: Assay Validation & Post-Validation Monitoring
CLSI C60: Assay Validation & Post-Validation Monitoring Ross J. Molinaro, MT(ASCP), PhD, DABCC, FACB Medical Director Core Laboratory, Emory University Hospital Midtown Emory Clinical Translational Research
More informationA Sub-picogram Quantification Method for Desmopressin in Plasma using the AB SCIEX Triple Quad 6500 System
A Sub-picogram Quantification Method for Desmopressin in Plasma using the AB SCIEX Triple Quad 6500 System A high-throughput method for detecting ultra-low levels (0.5 pg/ml) of a therapeutic peptide in
More informationUniversal Solution for Monoclonal Antibody Quantification in Biological Fluids Using Trap-Elute MicroLC-MS Method
Universal Solution for Monoclonal Antibody Quantification in Biological Fluids Using Trap-Elute MicroLC-MS Method Featuring the SCIEX QTRAP 6500+ LC-MS/MS System with OptiFlow Turbo V source and M5 MicroLC
More informationAPI 3200 LC/MS/MS system
www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment API 3200 LC/MS/MS system Reliability, Reproducibility, and Confidence in Your
More informationUltra-Sensitive Quantification of Trastuzumab Emtansine in Mouse Plasma using Trap-Elute MicroLC MS Method
Ultra-Sensitive Quantification of Trastuzumab Emtansine in Mouse Plasma using Trap-Elute MicroLC MS Method Featuring SCIEX QTRAP 6500+ System with OptiFlow Turbo V source and M5 MicroLC system Lei Xiong,
More informationAgilent 6490 Triple Quadrupole LC/MS System REACH ULTIMATE SENSITIVITY WITH BREAKTHROUGH IFUNNEL TECHNOLOGY
Agilent 6490 Triple Quadrupole LC/MS System REACH ULTIMATE SENSITIVITY WITH BREAKTHROUGH IFUNNEL TECHNOLOGY AGILENT 6490 TRIPLE QUADRUPOLE LC/MS SYSTEM WITH IFUNNEL TECHNOLOGY CLEARLY BETTER SENSITIVITY
More informationJohn Mehl, Bogdan Sleczka, Eugene Ciccimaro, Christian Caporuscio, Ekaterina Deyanova, Richard Huang, Timothy Olah, Celia D Arienzo
Bioanalytical strategies for the quantitation of in-vivo site specific modifications of therapeutic antibodies in early discovery cyno studies using IP-LC-MS John Mehl, Bogdan Sleczka, Eugene Ciccimaro,
More informationQuantification of Host Cell Protein Impurities Using the Agilent 1290 Infinity II LC Coupled with the 6495B Triple Quadrupole LC/MS System
Application Note Biotherapeutics Quantification of Host Cell Protein Impurities Using the Agilent 9 Infinity II LC Coupled with the 6495B Triple Quadrupole LC/MS System Authors Linfeng Wu and Yanan Yang
More informationSimultaneous Quantitation of a Monoclonal Antibody and Two Proteins in Human Plasma by High Resolution and Accurate Mass Measurements
Simultaneous Quantitation of a Monoclonal Antibody and Two Proteins in Human Plasma by High Resolution and Accurate Mass Measurements Paul-Gerhard Lassahn 1, Kai Scheffler 2, Myriam Demant 3, Nathanael
More informationAtmospheric Pressure Gas Chromatography: Background & Applications. Jody Dunstan MS Systems Evaluation, Manchester 2011 Waters Corporation 1
Atmospheric Pressure Gas Chromatography: Background & Applications Jody Dunstan MS Systems Evaluation, Manchester 2011 Waters Corporation 1 What is APGC? 2011 Waters Corporation 2 What is APGC Waters APGC
More informationBIOANALYSIS CONSUMABLE SOLUTIONS. Sample Preparation and Liquid Chromatography Solutions for Quantitative BIOANALYSIS
BIOANALYSIS CONSUMABLE SOLUTIONS Sample Preparation and Liquid Chromatography Solutions for Quantitative BIOANALYSIS At Waters, we understand the unique challenges faced by the bioanalytical community,
More informationQuantitative LC-MS Analysis of 14 Benzodiazepines in Urine Using TraceFinder 1.1 Software and High Resolution Accurate Mass
Application Note: 529 Quantitative LC-MS Analysis of 14 Benzodiazepines in Urine Using TraceFinder 1.1 Software and High Resolution Accurate Mass Xiang He, Marta Kozak; Thermo Fisher Scientific, San Jose,
More informationA Sub-picogram Quantification Method for Desmopressin in Plasma using the SCIEX Triple Quad 6500 System
A Sub-picogram Quantification Method for Desmopressin in Plasma using the SCIEX Triple Quad 6500 System A high-throughput method for detecting ultra-low levels (0.5 pg/ml) of a therapeutic peptide in human
More informationNew Approaches to Quantitative Proteomics Analysis
New Approaches to Quantitative Proteomics Analysis Chris Hodgkins, Market Development Manager, SCIEX ANZ 2 nd November, 2017 Who is SCIEX? Founded by Dr. Barry French & others: University of Toronto Introduced
More informationRobustness of the Agilent Ultivo Triple Quadrupole LC/MS for Routine Analysis in Food Safety
Technical Overview Robustness of the Agilent Ultivo Triple Quadrupole LC/MS for Routine Analysis in Food Safety Authors Mark Sartain, Theresa Sosienski, and Dan Hui Dorothy Yang Agilent Technologies, Inc.
More informationA TFC MS/MS Method for the Determination of the Cholinesterase Sensitive Analyte Rivastigmine in Human Plasma
White Paper A TFC MS/MS Method for the Determination of the Cholinesterase Sensitive Analyte Rivastigmine in Human Plasma Lars Neudert, MSc, Senior Scientist Method Development Laurence Meunier, PhD, Senior
More informationENVIRONMENTAL ANALYSIS. Solutions for Your Analytical Business Markets and Applications Programs. Solution Note
ENVIRONMENTAL ANALYSIS A TURN-KEY GUARANTEED ANALYSER FOR THE ROUTINE MEASUREMENT OF GLYPHOSATE AND AMPA IN DRINKING WATER (EUROPEAN DRINKING WATER DIRECTIVE 98/83/EC) AND OTHER ENVIRONMENTAL WATER SAMPLES
More informationImproving Sensitivity in Bioanalysis using Trap-and-Elute MicroLC-MS
Improving Sensitivity in Bioanalysis using Trap-and-Elute MicroLC-MS Using the SCIEX M3 MicroLC system for Increased Sensitivity in Antibody Quantitation Remco van Soest and Lei Xiong SCIEX, Redwood City,
More informationThe Agilent StreamSelect LC/MS Solution: Increasing the Throughput of a Triple Quadrupole Mass Spectrometer
The Agilent StreamSelect LC/MS Solution: Increasing the Throughput of a Triple Quadrupole Mass Spectrometer Technical Overview Introduction Liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS)
More informationDetermination of Table 3 Compounds by LC/MS/MS Chemours Fluoroproducts Analytical Method Revision date: 4/26/2018
Determination of Table 3 Compounds by LC/MS/MS Chemours Fluoroproducts Analytical Method Revision date: 4/26/2018 Instrument Setup (Note: Trapping column installed on Line A. Delay column installed between
More informationPharmacokinetic Analysis of the mab Adalimumab by ELISA and Hybrid LBA/LC/MS: A Comparison Study
Pharmacokinetic Analysis of the mab Adalimumab by ELISA and Hybrid LBA/LC/MS: A Comparison Study Featuring the SCIEX BioBA Solution Xi Qu 1, Shuyu Hou 1, Meghan McCann 1, Caroline Becker 1, Xun Wang 1,
More informationHDX-MS at Waters Waters Corporation 1
HDX-MS at Waters 2011 Waters Corporation 1 Hydrogen Deuterium Exchange MS systems Dedicated standards and UPLC BEH separation chemistries nanoacquity UPLC with HDX technology Xevo G2-S QTof MS and Synapt
More informationHigh Speed, Ultra-High Sensitivity, and Robustness Needed for the Quantitation of Pharmaceuticals in Blood Plasma. Application Note. Authors.
High Speed, Ultra-High Sensitivity, and Robustness Needed for the Quantitation of Pharmaceuticals in Blood Plasma Application Note Drug discovery and development: Drug metabolism and pharmacokinetics (DMPK)
More informationCAMAG. Jack Henion, Emeritus Professor Cornell University and VP of R&D. at Quintiles Bioanalytical and ADME Labs, USA
CAMAG DBS-MS 500 ONLINE EXTRACTION SYSTEM FOR LC-MS, MS OR auto COLLECTOR COUPLING The DBS-MS 500 handles the unattended analysis of up to 2000 DBS samples which is particularly beneficial when very large
More informationA Highly Accurate Mass Profiling Approach to Protein Biomarker Discovery Using HPLC-Chip/ MS-Enabled ESI-TOF MS
Application Note PROTEOMICS METABOLOMICS GENOMICS INFORMATICS GLYILEVALCYSGLUGLNALASERLEUASPARG CYSVALLYSPROLYSPHETYRTHRLEUHISLYS A Highly Accurate Mass Profiling Approach to Protein Biomarker Discovery
More informationHARMONIZATION TEAM A6 (STABILITY) UPDATE. Yoshiaki Ohtsu 8 March 2012
HARMONIZATION TEAM A6 (STABILITY) UPDATE Yoshiaki Ohtsu 8 March 2012 Contents 1. General update 2. Scientific discussion point 1. Stability of presence of co administered or co formulated drugs 2. Incurred
More informationThe Agilent Metabolomics Dynamic MRM Database and Method
The Agilent Metabolomics Dynamic MRM Database and Method White Paper Author Mark Sartain Agilent Technologies, Inc. Santa Clara, California, USA Introduction A major challenge in metabolomics is achieving
More informationAgilent Automated Card Extraction Dried Blood Spot LC/MS System
Agilent Automated Card Extraction Dried Blood Spot LC/MS System Quick Start Guide What is the AACE DBS LC/MS System? 2 System Overview 3 Operational Overview 4 Method Development 5 Using the AACE DBS LC/MS
More informationProudly serving laboratories worldwide since 1979 CALL for Refurbished & Certified Lab Equipment
www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +1.847.913.0777 for Refurbished & Certified Lab Equipment API 3000 LC/MS/MS Unprecedented Performance: An advanced LC/MS/MS system
More informationNewborn Screening for Nine Lysosomal Storage Diseases
Newborn Screening for Nine Lysosomal Storage Diseases Michael H. Gelb, Frank Turecek, C. Ron Scott Zdenek Spacil, Brian Wolfe, Trisha Duffey, Sophie Blanchard Univ. of Washington 2004: We reported a 5-plex
More information10. Validated Normal Phase HPLC Method for the Determination. Fulvestrant is primarily used in the treatment of hormone receptor
229 10. Validated Normal Phase HPLC Method for the Determination of Fulvestrant in Pharmaceutical Dosage Forms 10.1 Introduction Fulvestrant is primarily used in the treatment of hormone receptor positive
More informationUpdating Solid Phase Extraction Methods: Tips and Tricks for Improving Existing and New SPE Methods using Method Development
Updating Solid Phase Extraction Methods: Tips and Tricks for Improving Existing and New SPE Methods using Method Development Golnar Javadi Application Engineer Sample Preparation Products Spp-support@agilent.com
More informationMicroflow Liquid Chromatography Mass Spectrometry System. Nexera Mikros C146-E350
Microflow Liquid Chromatography Mass Spectrometry System Nexera Mikros C146-E35 Micro: Above and Beyond Nano The High Sensitivity You Expect from a Low Flow System with the Ruggedness of HPLC Covering
More informationInformatics and High Resolution QTof MS. How can we ask better questions and get better answers in DMPK? Mark D. Wrona
Informatics and High Resolution QTof MS How can we ask better questions and get better answers in DMPK? Mark D. Wrona Principal Scientist Pharmaceutical & Life Sciences, Waters Corporation Pacific Northwest
More informationProudly serving laboratories worldwide since 1979 CALL for Refurbished & Certified Lab Equipment QTRAP 5500
www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment QTRAP 5500 Powerful synergy results when the world s most sensitive triple
More informationRapid Soft Spot Analysis using the SCIEX Routine Biotransform Solution
Rapid Soft Spot Analysis using the SCIEX Routine Biotransform Solution Rapidly Identify Top Metabolites with the SCIEX X500R QTOF System and MetabolitePilot TM 2.0 Software Shaokun Pang and Ian Moore SCIEX,
More informationDirect PCR Amplification of Blood Samples on Copan NUCLEIC-CARD TM Systems Using the AmpFlSTR Identifiler Direct PCR Amplification Kit
Technical Focus Direct PCR Amplification of Blood Samples on Copan NUCLEIC-CARD TM Systems Using the AmpFlSTR Identifiler Direct PCR Amplification Kit Nicola Oldroyd, Jennifer Schroeder, Dennis Wang, Alessandro
More informationChapter 2 deals with LC-ESI-MS/MS studies on forced degradation of abacavir sulphate an anti HIV drug.
The drug discovery and development process has undergone dramatic changes particularly in the last decade. Progress in drug discovery has been fuelled by improvements in methodologies and technologies
More informationWaters Pesticide Screening Solutions: Nuove Frontiere nell Analisi di pesticidi Miguel Ángel Cortés, Waters Manuela Cuccurullo, Waters
Waters Pesticide Screening Solutions: Nuove Frontiere nell Analisi di pesticidi Miguel Ángel Cortés, Waters Manuela Cuccurullo, Waters MS Technology day, Milan March 12 2013 Waters Corporation 1 The purpose
More information[ WHITE PAPER ] High Throughput Microflow LC-MS: Sensitivity Gains on a Practical Timescale ABSTRACT INTRODUCTION
High Throughput Microflow LC-MS: Sensitivity Gains on a Practical Timescale Michael Donegan, James Murphy, and Angela Doneanu Waters Corporation, Milford, MA, USA ABSTRACT The sensitivity gains observed
More information[ APPLICATION NOTE ] APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS KEYWORDS
Quantitative Method for the Determination of Potentially Mutagenic Impurities of Ondansetron Using UPC 2 Coupled with a Xevo TQ-S micro Jennifer Simeone, Paula Hong, and Patricia R. McConville Waters Corporation,
More informationDETERMINATION OF PROTEIN BINDING BY UPLC/MS/MS
DETERMINATION OF PROTEIN BINDING BY UPLC/MS/MS Darcy Shave and Pete Alden Waters Corporation, Milford, MA, U.S.A. INTRODUCTION Plasma protein binding (PPB) can significantly affect the therapeutic action
More informationRapid Determination of Drug Protein Binding Affinity using Solid Phase Microextraction
Rapid Determination of Drug Protein Binding Affinity using Solid Phase Microextraction Len Sidisky, Craig Aurand, Emily Barrey, Candace Price, and David Bell MilliporeSigma, Bellefonte, PA, USA T416070
More informationfor water and beverage analysis
Thermo Scientific EQuan MAX Plus Systems Automated, high-throughput LC-MS solutions for water and beverage analysis Pesticides Pharmaceuticals Personal care products Endocrine disruptors Perfluorinated
More informationOptiFlow Quant Solution for High-Sensitivity Quantitation
OptiFlow Quant Solution for High-Sensitivity Quantitation The Intelligent Union of Sensitivity, Ease-of-Use, and Robustness MicroFlow Sensitivity Analytical Flow Usability Transform Your Quantitation Microflow
More informationRapid Extraction of Therapeutic Oligonucleotides from Primary Tissues for LC/ MS Analysis Using Clarity OTX, an Oligonucleotide Extraction Cartridge
Rapid Extraction of Therapeutic Oligonucleotides from Primary Tissues for LC/ MS Analysis Using Clarity OTX, an Oligonucleotide Extraction Cartridge G. Scott*, H. Gaus #, B. Rivera*, and M. McGinley* *Phenomenex,
More informationMethod Development of a 2D LC-HRMS Extraction and Detection Method for Organophosphorus Flame Retardants in Environmental Water Samples
Method Development of a 2D LC-HRMS Extraction and Detection Method for Organophosphorus Flame Retardants in Environmental Water Samples Ken Rosnack 1 Lauren Mullin 1,2, Malorie Mella 1, Claude Mallet 1,
More informationMultiQuant Software 2.0 with the SignalFinder Algorithm
MultiQuant Software 2.0 with the SignalFinder Algorithm The Next Generation in Quantitative Data Processing Quantitative analysis using LC/MS/MS has benefited from a number of advancements that have led
More informationSYMBIOSIS LAB EFFICIENCY OF UNLIMITED DIMENSIONS
SYMBIOSIS LAB EFFICIENCY OF UNLIMITED DIMENSIONS ABOUT SPARK SPARK HOLLAND IS WORLD-CLASS PROVIDER OF INNOVATIVE SAMPLE INTRODUCTION, EXTRACTION AND SEPARATION TECHNOLOGY FOR HPLC, MS, GC AND NMR SYSTEMS.
More informationSpecific Presentations and Discussion Topics for The 1 st CRO Closed Forum (Montreal, Sept. 14 th 2010)
Specific Presentations and Discussion Topics for The 1 st CRO Closed Forum (Montreal, Sept. 14 th 2010) Advion Interfacing with the FDA on issued 483 s an Opportunity to Discuss Science and Address Misunderstandings?
More information[ product solution ] Waters Oasis µ Elution PlateS. Patented Innovation. Elution volume as low as 25 μl. No evaporation and reconstitution
[ product solution ] Waters µ Elution PlateS Elution volume as low as 25 μl No evaporation and reconstitution Ideal for small sample volumes Up to a 15x increase in concentration Patented Innovation Now
More informationValidation of Analytical Method of Irbesartan Plasma in Vitro by High Performance Liquid Chromatography-Fluorescence
Journal of Life Sciences 6 (2012) 726-731 Validation of Analytical Method of Irbesartan Plasma in Vitro by High Performance Liquid Chromatography-Fluorescence Harmita, Yahdiana Harahap and I. Kadek Arya
More informationQuantification of genotoxic "Impurity D" in Atenolol by LC/ESI/MS/MS with Agilent 1200 Series RRLC and 6410B Triple Quadrupole LC/MS
Quantification of genotoxic "Impurity D" in Atenolol by LC/ESI/MS/MS with Agilent 12 Series RRLC and 641B Triple Quadrupole LC/MS Application Note Manufacturing Process Development Author Siji Joseph Agilent
More informationENHANCED MS WITH THE TURN OF A KEY
[ ionkey/ms ] ENHANCED MS WITH THE TURN OF A KEY EXPERIENCE THE FUTURE OF MS, EXPERIENCE ionkey/ms THE LC-MS YOU KNOW, LIKE YOU VE NEVER SEEN BEFORE ionkey/ms Systems integrate the UPLC separation into
More informationAbout HFL. Markets & services. Markets & services. Qualitative MS: Pharmaceutical Applications. Regulatory Framework
Certainty of Identification in Pharmaceutical and Forensic Mass Spectrometry Phil Teale, HFL Ltd. About HFL The only laboratory in the world engaged in sports doping control & contract research Long heritage
More informationDirect Quantitative Bioanalysis of Drugs in Dried Blood Spot Samples
Direct Quantitative Bioanalysis of Drugs in Dried Blood Spot Samples Paul Abu-Rabie Bioanalytical Science and Development, PTS DMPK, GlaxoSmithKline, Ware, UK (Paul.2.Abu-Rabie@gsk.com) Dried Blood Spots
More informationGenotoxicity is the property of a compound
Impurities Analysis in Pharmaceuticals: Genotoxicity is the property of a compound known to have irreversible effects on the structure and functionality of the DNA in cells and cause DNA loss, DNA replication
More informationHigh Levels of Sensitivity and Robustness for Complex and Demanding Matrices SCIEX TRIPLE QUAD 5500 SYSTEM
High Levels of Sensitivity and Robustness for Complex and Demanding Matrices SCIEX TRIPLE QUAD 5500 SYSTEM The SCIEX Triple Quad 5500 System is designed to deliver a high level of sensitivity and robustness
More informationThe Application of SPME; A Multipurpose Micro-Technique for Bioanalysis
EBF Hatching Brussels 12-13 June 2012 The Application of SPME; A Multipurpose Micro-Technique for Bioanalysis Sheelan Ahmad Bioanalytical Science and Toxicokinetics, PTS DMPK, GlaxoSmithKline, Ware, UK
More informationStrategies for Phospholipid Removal using Polymer-based SPE
Strategies for Phospholipid emoval using Polymer-based SPE Lee Williams, Helen Lodder, Matthew Cleeve, Scott Merriman, Steve Jordan, ichard Calverley, Steve Plant, Joanna Smith Introduction Sample preparation
More informationSee More, Much More Clearly
TRIPLETOF 6600 SYSTEM WITH SWATH ACQUISITION 2.0 See More, Much More Clearly NEXT-GENERATION PROTEOMICS PLATFORM Every Peak. Every Run. Every Time. The Next-generation Proteomics Platform is here: The
More informationImproving Sensitivity for an Immunocapture LC-MS Assay of Infliximab in Rat Plasma Using Trap-and-Elute MicroLC-MS
Improving Sensitivity for an Immunocapture LC-MS Assay of in Rat Plasma Using Trap-and-Elute MicroLC-MS Using the SCIEX M3 MicroLC system for Increased Sensitivity in Antibody Quantitation Remco van Soest
More informationHow discovery activities can influence metabolic profiling in the regulatory space? C. DELATOUR EBF, 25 th September 2015
How discovery activities can influence metabolic profiling in the regulatory space? C. DELATOUR EBF, 25 th September 2015 Outline 2 ן Background - Objectives - Shift in Workflow - Metabolite in safety
More informationA fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH) 2 D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS
A fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH) 2 D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS N. Fabregat-Cabello, J. Farré-Segura, L. Huyghebaert,
More informationSolutions Guide. MX Series II Modular Automation for Nano and Analytical Scale HPLC And Low Pressure Fluid Switching Applications
Solutions Guide MX Series II Modular Automation for Nano and Analytical Scale HPLC And Low Pressure Fluid Switching Applications Page 1 of 12 Table of Contents Sample Injection... 3 Two- Selection... 4
More information