ENHANCED MS WITH THE TURN OF A KEY

Transcription

1 [ ionkey/ms ] ENHANCED MS WITH THE TURN OF A KEY

2 EXPERIENCE THE FUTURE OF MS, EXPERIENCE ionkey/ms THE LC-MS YOU KNOW, LIKE YOU VE NEVER SEEN BEFORE ionkey/ms Systems integrate the UPLC separation into the source of the mass spectrometer, delivering a level of performance not achievable any other way. It is the UPLC separations you already know, robust, reproducible, and reliable, tried and tested by the world s most demanding laboratories, now easier to use and more sensitive. ionkey/ms Systems provide users with: Increased sensitivity Simplified user experience The power to perform multiple analyses with limited sample volumes The power to analyze smaller sample sizes Reduced solvent consumption WITH ionkey/ms, WATERS TRANSFORMS WHAT YOUR LABORATORY CAN DO A wide range of laboratories are benefitting from the use of ionkey/ms, including: Drug discovery and development laboratories Contract laboratories Food and environmental testing laboratories Academic research laboratories Core/central laboratories ionkey/ms MAKES ASSAYS WHICH WERE NOT POSSIBLE IN A ROUTINE LAB, POSSIBLE I was blown away by the increase in sensitivity. 3 DMPK scientists got >8-fold improvement in sensitivity compared to 2.1 mm I.D. chromatography on our first attempt. Phil Tiller, RMI Laboratories

3 [ ionkey/ms ] A GIANT LEAP IN LC-MS PERFORMANCE ionkey/ms Systems redefine ultimate LC-MS performance by physically integrating the UPLC separation into the source of the mass spectrometer, delivering a level of sensitivity, chromatographic performance, and ease-of-use that is unachievable by any other LC-MS System. Only Waters combination of scientific excellence and keen understanding of customer needs could produce ionkey/ms, integrating: UPLC separations ionkey Source with the ikey Separation Device Mass Spectrometry Designed for the most demanding qualitative and quantitative applications, Waters mass spectrometers provide unprecedented levels of sensitivity and the ultimate in quantitative performance. They feature patented StepWave technology a revolutionary off-axis ion source technology that provides ultimate sensitivity and maximum robustness. INTEGRATED WITH THE ACQUITY UPLC M-CLASS, ionkey/ms. IT S ALL ABOUT THE SYSTEM. The ionkey/ms System with the ACQUITY UPLC M-Class and the Xevo TQ-S Mass Spectrometer.

4 ENHANCED SENSITIVITY, GROUNDBREAKING PERFORMANCE SENSITIVITY COMPARISON BETWEEN ionkey/ms AND 2.1 MM I.D. CHROMATOGRAPHY; 1 μl INJECTION OF EQUAL SAMPLE LOAD ON EACH Alprazolam OH Clopidogrel 1 ionkey/ms 2.1 mm I.D. ionkey/ms 2.1 mm I.D. 4X 2X Oxytocin REDUCE SAMPLE LOAD AND MAINTAIN SENSITIVITY 1 ionkey/ms T43 ENL peptide 1 ionkey/ms 27X mm I.D. 2.1 mm I.D. 24X ionkey/ms provides significant increases in S:N compared to 2.1 mm I.D. chromatography allowing scientists to reach lower limits of detection and see things they could not see before. ionkey/ms and 2.1 mm I.D. peaks are time aligned for comparison purposes UPLC/MRM comparison of ionkey/ms relative to 2.1 mm I.D. chromatography Compound name Relative peak area Small Molecule Relative S/N Lidocaine 3 3 Fluconazole Propanolol 2 2 Alprazolam OH 14 4 Alprazolam 7 12 Nefazodone 3 6 Tolbutamide 5 6 Warfarin 2 4 Clopidogrel 2 2 Mean value 6 13 UPLC/MRM comparison of ionkey/ms relative to 2.1 mm I.D. chromatography Compound name Relative peak area Peptides Relative S/N T23 ENL T4 ENL T43 ENL Oxytocin Octreotide 8 1 T11 ENL T38 ENL 1 11 T16 ENL 5 4 T14 ENL 5 3 Mean value X less sample mm x 1 cm 2 µl Injection ionkey/ms x 1 cm.5 µl Injection Injection volume ionkey/ms ACQUITY 2.1 mm I.D. Chromatography.5 µl 2 µl Signal 3.75E E+5 Noise S/N Area ionkey/ms enables scientists to reduce sample volumes and maintain the S:N compared to 2.1 mm I.D. chromatography. This allows scientists to reduce sample volumes or perform multiple analyses when they have limited samples. Gradient separation of 5 ng/ml of desmopressin.

5 [ ionkey/ms ] EASE OF USE EXPANDS YOUR OPPORTUNITIES ionkey/ms Systems are enabled by the innovative ikey, which replaces the need for traditional fittings and columns and simplifies the user experience. The plug-and-play design eliminates user-made connections and the associated operator variability, providing a superior level of confidence and enables users at any level of experience to obtain high quality data. The ikey contains the fluidic connections, electronics, ESI interface, column heater, ecord, and the chemistry to perform UPLC separations in the source of Waters mass spectrometers. It makes the most sensitive LC-MS instrument on the market accessible to any user regardless of skill level. I strongly believe that systems combining ease of use and optimum microflow performance, exemplified by Waters ionkey/ms, will be the next wave in both routine and advanced LC/MS analysis. Rand Jenkins, PPD Simply insert the ikey and go! Electrical connections and column heating control Electrospray connection contact Robust emitter Nebulization gas inlet Fluid entry connections ikey shown is actual size The ikey is available with a variety of sorbents. Every ikey is LC-MS quality control tested, so you can be confident in your analysis. For detailed specifications and ordering information, visit

6 THE UPLC SEPARATIONS YOU KNOW ionkey/ms provides the same UPLC chromatographic performance you know robust, reproducible, and reliable in a 15 μm I.D. channel packed with sub-2 μm particles. UPLC CHROMATOGRAPHIC PERFORMANCE Overlay showing MRM chromatograms of 99 pesticides spiked into an infant formula extract. Peak capacity is critical in food and environmental testing in order to contend with the large number of analytes and the high level of matrix complexity. The ability to increase the analyte response, without having to inject more sample, offers improved detection limits whilst avoiding higher matrix loads. REPRODUCIBLE FROM USER TO USER, SYSTEM TO SYSTEM Each ikey is LC-MS tested to ensure performance not only within an ikey but from one ikey to another. 1user X 1system X 1iKey Peptide from P924, yeast enolase 6.84 min Retention time 6.69 min.1 min SD of retention time.11 min 3.1 sec Average peak width FWHM 2.72 sec 5 users X 5 systems X 18 ikeys retention time RSD QC reproducibility for one ikey with over 5 injections of protein precipitated plasma. retention time RSD QC reproducibility across multiple users, ionkey/ms systems, and ikeys.

7 ROBUST: HUNDREDS OF INJECTIONS, IDENTICAL RESULTS 1 First 5 µl injection 1 After 1 5 µl injections of crashed plasma Quality control injections of dextromethorphan and propanolol showing equivalent chromatographic resolution before study and after 1 x 5 µl injections of 2:1 protein precipitated plasma. 7 First injection Peak area RSD: psi 3 2 Last injection Injection Number 12 Consistent pressure trace before study and after 1 x 1 µl injections of 2:1 protein precipitated plasma samples. Peak area reproducibility over 1 x 1 μl injections of 2:1 protein precipitated plasma samples. Waters has a complete portfolio of standards, certified vials, and certified containers for your ionkey/ms System. For more information, visit The ionkey/ms System with the ACQUITY UPLC M-Class and the SYNAPT G2-Si HDMS Mass Spectrometer.

8 [ ionkey/ms ] ENIVRONMENTAL, ETHICAL, AND ECONOMICAL ionkey/ms Systems significantly reduce the amount of solvent consumed compared to 2.1 mm I.D. scale analyses. That translates into: Lower solvent costs Reduced storage costs Reduced disposal costs More sustainable workflow for the environment The table below illustrates the reduction of the cost in the analysis of one 96-well plate utilizing a 2.1 mm I.D. column compared to a 15 µm I.D. column, geometrically scaled and based on the cost of 1 L of acetronitrile costing $123.. We had tremendous gains in solvent waste reduction, over 9. In some applications, it s almost 98 of solvent waste reduction. Arjen Gerssen, RIKILT Institute for Food Safety Column I.D./flow rate Organic (ml consumed during cycle steps) Total ml consumed Cost/96-well plate 2.5 min gradient.5 min wash 2 min condition 2.1 mm.8 ml/min $ µm.4 ml/min $.9 DO MORE WITH LESS SAMPLE ionkey/ms Systems give you the power to analyze smaller samples and do more when you have limited samples. Save time and money by reducing the amount of sample to be prepared and analyzed Minimize the use of costly standards Potentially eliminate the need for matrix matched standards by diluting sample matrix Improve MS uptime and robustness by reducing sample and solvent load When you have limited sample volumes, gain more information and confidence by: 1. Performing more analyses on the same sample BE ASSURED. CHOOSE WATERS GLOBAL SERVICES. Waters Global Services focuses on optimizing Waters products with superior service, support, upgrades, training, and Waters Quality Parts. Proven satisfaction For 13 consecutive years, an independent quality auditing firm has ranked Waters Global Services as Bestin-Class in providing expert technical knowledge, quick resolution of system issues, and process support Achievement in Customer Excellence Award, CustomerSat, Inc., ; NorthFace ScoreBoard Awards sm, Omega Management Group Corporation, Reducing variation in data by eliminating the need to pool samples

9 [ ionkey/ms ] EXPAND YOUR POSSIBILITIES The ionkey/ms System with the ACQUITY UPLC M-Class and the Xevo G2-XS QTof Mass Spectrometer. The unique and innovative ionkey/ms System has been embraced in the scientific community and those scientists using it are enjoying the benefits. See the proof and discover how ionkey/ms can expand your possibilities in your laboratory by visiting THE CHROMATOGRAPHY THAT YOU KNOW THE INSTRUMENTS THAT YOU TRUST THE EASE OF USE THAT YOU NEED THE SENSITIVITY THAT YOU ASKED FOR

10 SALES OFFICES: Austria Australia Belgium and Luxembourg Brazil Canada China Czech Republic Denmark Finland France Germany Hong Kong Hungary India Ireland Israel Italy Japan Korea Mexico The Netherlands Norway Poland Portugal Puerto Rico Russia/CIS / Singapore Spain Sweden Switzerland Taiwan UK US Waters Corporation 34 Maple Street Milford, MA 1757 U.S.A. T: F: Waters, The Science of What s Possible, Waters Quality Parts, UPLC, Xevo and ACQUITY are registered trademarks of Waters Corporation. ionkey /MS, ionkey, ikey, StepWave and ecord are trademarks of Waters Corporation. All other trademarks are the property of their respective owners. 214 Waters Corporation. Printed in the U.S.A. October EN TC-KP