Reversed Phase Solutions for the Analysis of Proteins, Peptides, and Oligonucleotides

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1 Reversed Phase Solutions for the Analysis of Proteins, Peptides, and Oligonucleotides The line, which includes and Proteo, offers various reversed phase solutions for biochromatography. With these two columns, one can identify, purify, and analyze almost any protein or oligonucleotide. Å column designed to analyze intact proteins and large biomolecules Proteo 9 Å column designed to increase the peak capacity and resolution of peptide maps as well as separate peptides and small oligonucleotides (<mer) f does not provide you with at least an equivalent separation as compared to a column of similar phase, particle size and dimension, send in your comparative data within 5 days and keep the column for FREE. Material Characteristics Calculated Packing Particle Pore Pore Surface Carbon Bonded Phase End Material Shape/Size Size Volume Area Load Coverage Capping (μm) (Å) (ml/g) (m /g) % (µmole/m ) C Spher. 5,, Yes C5 Spher. 5,, Yes C8 Spher. 5,, Yes Proteo Spher., Yes For Large Protein, Polypeptide, and Large Oligonucleotide Analysis/ Purification Excellent Å column for large proteins, polypeptides (MW>,) and oligonucleotides > mer Stable from ph.5 to ( hours) 5, and 5 μm bonded phases available for convenient scale-up Extensive batch traceability data provided with every column Sharp Peaks and High Yields is designed to analyze and purify intact proteins and macromolecules. Ultra-pure (99.99 % metal-free) silica and dense bonded phase coverage provide sharp peaks for proteins. Dense bonded phase coverage combined with a relatively high silica surface area yields a high capacity sorbent for high loadability. is an excellent column for biological purification. Chromatographic Comparisons of 5 μm, C, Å 5 x.6 mm Columns*, ** App D 565 App D Vydac 6 8 min YMC-Pack 6 8 min min SynChropak min App D 568 App D 567 Peptide Hormones** Conditions for all columns: 5 x.6 mm A:. % TFA in Water B:. % TFA in Acetonitrile/Water (9:) Gradient: a) A/B (9:) to A/B (7:6) in 8 min ( % B/min) b) A/B (7:6) to A/B (7:) in 6 min (.57 % B/min) Detection: Sample:. ml/min nm. [Arg 8 ]-Vasotocin (Cys-Tyr-le-Gln-Asn- Cys-Pro-Arg-Gly-NH ). [Arg 8 ]-Vasopressin (Cys-Tyr-Phe-Gln-Asn- Cys-Pro-Arg-Gly-NH ). sotocin (Cys-Tyr-le-Ser-Asn- Cys-Pro-le-Gly-NH ). Oxytocin (Cys-Tyr-le-Gln-Asn- Cys-Pro-Leu-Gly-NH ) *This comparative data may not be representative for all applications. **Better results might be achieved by columns of different pore sizes. is a registered trademark of Phenomenex, nc. column manufactured by Phenomenex. Phenomenex is a registered trademark of Phenomenex, nc. Vydac is a registered trademark of Alltech Associates, nc. Vydac column purchased from Grace Vydac. Phenomenex is not associated with Grace Vydac. Zorbax is a registered trademark of Agilent Technologies. Zorbax column purchased from MAC-MOD. Phenomenex is not associated with Agilent Technologies or MAC-MOD. YMC is a registered trademark of YMC Co., Ltd. YMC column purchased from YMC. Phenomenex is not associated with YMC Co., Ltd. SynChropak is a registered trademark of Eprogen, nc. SynChropak column purchased from Eichrom Technologies, nc. Phenomenex is not associated with Eichrom Technologies, nc. nor Eprogen, nc. Phenomenex

2 (cont'd) ph.5 - Stability High bonded phase surface coverage and uniformity is achieved using our bonding process. Dense bonding provides ph stability from ph.5 to for more than hours of use. This exceptional ph stability allows for easy column cleaning as well as the use of mobile phases that maintain protein biologic activity. Low TFA Conditions TFA can actually be a drawback when electrospray (ES) interfaces are used to introduce samples into the mass spectrometer. At typical working concentrations (. %), TFA has been shown to greatly reduce ion generation in ES-MS, presumably due to a combination of its ion-pairing capacity as well as its role as a competitive ion. The use of TFA does provide excellent peak shapes, but at the cost of reduced sensitivity in LC/ES-MS., however, provides excellent peak shapes for proteins even at reduced concentrations (. % TFA) without compromising peak symmetry. Rapid LC/MS Analysis Reduce analysis time, solvent usage and column equilibration time by as much as 7 % for some applications ncrease sample throughput by as much as 7 % while maintaining excellent sensitivity, resolution and the accuracy of your Mass Spec data Below are MS results for protein and peptide samples comparing 5 μm Å LC/MS 5 x. mm columns to more conventional 5 μm Å 5 x. mm columns. LC/MS (C8) Sample: MS: Scan Range: Myoglobin Tryptic Digest HP in sec Total on Chromatograms App D % reduction in analysis time App D 8 Proteins Run Under. % TFA Column: 5 μm C Å 5 x.6 mm G-67-E ml/min A:. % TFA in Water B:. % TFA in Acetonitrile Gradient: A/B (75:5) to A/B (5:95) in min Detection: nm Temperature: 5 C Sample:. nsulin. Trypsinogen. Lactalbumin. Myoglobin 5. Carbonic anhydrase A B A 5 x. mm 5 min Mass Spectra of Selected Myoglobin Peptide App D x. mm Near identical results 5 x. mm App D 86 Proteins Run Under. % TFA Column: 5 μm C Å 5 x.6 mm G-67-E ml/min A:. % TFA in Water B:. % TFA in Acetonitrile Gradient: A/B (75:5) to A/B (5:95) in min Detection: nm Temperature: 5 C Sample:. nsulin. Trypsinogen. Lactalbumin. Myoglobin 5. Carbonic anhydrase B A Column: 5 x. mm m/z 5 μm C8 Å 5 x. mm G-5-B. ml/min A:. % TFA in Water B:. % TFA in Acetonitrile A/B (95:5) to A/B (5:95) in 6 min B Column: 5 μm C8 Å 5 x. mm B-5-B. ml/min A:. % TFA in Water B:. % TFA in Acetonitrile A/B (95:5) to A/B (5:95) in 5 min Phenomenex

3 (cont'd) Cut Analysis Time by as Much as 7 % Reduce solvent usage and column cost Maintain excellent resolution by utilizing steeper gradients Excellent as method development or screening columns The mechanism of retention and separation of large molecules like polypeptides by reversed-phase is primarily adsorption/ desorption, although partitioning appears to play a minor role. When using shorter columns (5 mm), reduced analysis times require steeper gradients to reach the same critical organic concentration. This is necessary for desorption of polypeptides in a shorter time compared to those used on longer length columns. App D 578 Proteins on C Column: 5 μm C Å. ml/min Detection: nm njection: 5 µl Sample:. Alkaline phosphatase. Cyanocobalamin. RNase. nsulin 5. Transferrin 6. Trypsin nhibitor A Rt=5 min 7 % reduction in analysis time and savings 5 6 Large Oligonucleotides - Separation and Purification provides excellent separation for oligonucleotides in excess of residues. Such separation allows one to quickly purify large oligonucleotides from failed sequence contaminants and other oligonucleotides. The following applications demonstrate the resolving power of in separating large oligonucleotides from contaminants generated during synthesis. App D 65 mer Oligonucleotide Separation Column: 5 μm C8 Å Dimension: 5 x.6 mm F-5-E A) mm TEAA in Water, ph 7. B) mm TEAA in Acetonitrile, ph 7. Gradient: A/B (96:) to A/B (85:5) in min ml/min Temperature: 5 C Detection: 6 nm Sample:. Failed sequence contaminant. mer Oligonucleotide 6 min B Rt=5 min 56 5 x.6 mm App D 67 6mer Oligonucleotide Separation Column: 5 μm C8 Å Dimension: 5 x.6 mm F-5-E A) mm TEAA in Water, ph 7. B) mm TEAA in Acetonitrile, ph 7. Gradient: A/B (96:) to A/B (85:5) in min ml/min Temperature: 5 C Detection: 6 nm Sample:. Failed sequence contaminant. 6mer Oligonucleotide 5 x.6 mm min A B Gradient: Gradient: 5 x.6 mm G-67-E A:. % TFA in Water B:. % TFA in Acetonitrile a) A/B (95:5) to A/B (7:6) in 7 min ( % B/min) b) A/B (7:6) to A/B (66:) in min (.67 % B/min) c) A/B (66:) to A/B (6:5) in min ( % B/min) 5 x.6 mm B-67-E A:. % TFA in Water B:. % TFA in Acetonitrile a) A/B (:) to A/B (8:) in min ( % B/min) b) A/B (8:) to A/B (65:5) in.5 min ( % B/min) c) A/B (65:5) to A/B (5.5:6.5) in.5 min (7.67 % B/min) d) A/B (5.5:6.5) to A/B (5.5:6.5) in min (constant B) min Phenomenex 5

4 (cont'd) Quality Proven Quality is carefully maintained and traceability is assured throughout the manufacturing process. Each column is shipped with extensive batch traceability data verifying batch quality, as well as with its own individual test chromatogram. Reproducibility Assured To demonstrate the reproducibility of the results obtained on different batches of material, Cytochrome c variants were used for their similar chemical structures. Consistency of resolution factors, capacity factors, retention times and peak asymmetries for these difficult-to-resolve variants were put to the test for you to judge. A Materials Validation Document (MVD) accompanies every column. Each certificate contains information on the rigorous testing procedures performed on each batch of material to ensure column-to-column and batch-to-batch reproducibility. The information below is provided with each column. Particle Analysis - Particle Size - Pore Diameter - Particle Size Distribution - Surface Area Total Metal Content Bonded Phase Coverage - Total Carbon - Surface Coverage Diagnostic Chromatography Test - nertness - Metal Sensitivity - Hydrophobic ndex Performance Chromatography Test - Protein Standards - Longevity Tests (ph Stability) - Scanning Electron Microscopy (measures surface smoothness and particle shape) -to- Reproducibility Column: Gradient: Detection: Sample: 5-5 μm C8, Å 5 x.6 mm G-5-E A:. % TFA in Water B:. % TFA in Acetonitrile A/B (75:5) to A/B (5:55) in 5 min ( % B/min). ml/min nm. Equine Cytochrome C. Bovine Cytochrome C. Canine Cytochrome C App D 557 App D min 6 Phenomenex

5 (cont'd) App D 559 RNA Fragments Column: 5 μm C Å 5 x.6 mm G-67-E A:. M K PO in.75 % sopropanol, ph 6.8 B:. M (NH ) SO +. M K PO in.75 % sopropanol, ph 6.8 Gradient: - % B for minutes, - % B for minutes, % B for minutes. ml/min Detection: 6 nm Sample: trna Fragments App D 99 Reduced Dog gg Column: 5 μm C Å 5 x. mm F-67-B A:. % TFA in Water B:.85 % TFA in Water/ Acetonitrile/PA (5:75:) Gradient: A/B: 8: to A/B: 5:95 in minutes.5 ml/min Detection: nm Sample: gg Dog Reduced 6 8 min App D 57 Proteins Column: Gradient: Detection: Sample: 5 μm C Å 5 x.6 mm B-67-E A:. % TFA in Water B:. % TFA in Acetonitrile a) A/B (:) to A/B (8:) in min ( % B/min) b) A/B (8:) to A/B (65:5) in.5 min ( % B/min) c) A/B (65:5) to A/B (5.5:6.5) in.5 min (7.67 % B/min) d) A/B (5.5:6.5) to A/B (5.5:6.5) for min (constant B). ml/min nm. Alkaline Phosphatase. Cyanocobalamin. RNase. nsulin 5. Transferrin 6. Trypsin nhibitor App D 57 nsulin Genetic Variants Column: Gradient: Detection: Sample: 5 μm C8 Å 5 x.6 mm G-5-E A:. % TFA in Water B:. % TFA in Acetonitrile A/B (7:) to A/B (68:) in min. ml/min nm. Bovine nsulin. Human nsulin. Porcine nsulin mau 5 mau min min Phenomenex 7

6 Proteo For protein digest, peptide mapping, synthetic peptides, and oligonucleotides < mer Excellent 9 Å pore column for separating polypeptide fragments Stable from ph.5 to Excellent peak symmetry in other modifiers besides TFA and μm particle available from capillary to preparative columns Extensive column-to-column and batch-to-batch reproducibility data provided ncrease Peak Capacity by -5 % Proteo is designed to selectively separate and to optimize information on peptide fragments obtained in a protein digest, which is a standard method of protein characterization. n Figure, a Proteo column offers better selectivity and resolving power in every aspect compared with several commercial Å, silica-based C8 reversed-phase columns traditionally employed for the analysis of protein digests and synthetic peptides. Further, special bonding technology permits the use of less trifluoracetic acid or other ion-pair agents without compromising peak symmetry and efficiency. Figure : Myoglobin Tryptic Digest Comparisons App D 8 μm Proteo 9 Å Avg peaks Zorbax 5 μm SB C8 Å Avg App D 8 peaks Myoglobin Tryptic Digest Conditions for all columns: 5 x.6 mm A). % TFA in Water B). % TFA in Acetonitrile Gradient: A/B (95:5) for 5 min, then to A/B (6:) in 55 minutes ml/min Temperature: C Detection: nm Sample: Myoglobin Tryptic Digest Vydac 5 μm MS C8 Å Avg peaks App D Determining peak counts - The large number of peaks in a given tryptic digest makes counting peaks visually both inaccurate and subjective. For a more accurate approach, peak counting was performed using Agilent Technologies (HP) ChemStation software. Four different integration parameters at different sensitivity settings were used in calculating the number of peaks and an average. The parameters changed within each method were: minimum peak area, minimum peak height, peak width, and threshold. The table below describes the parameters used for each calculation. Method Threshold Peak Width Min Area Min Height Phenomenex

7 Proteo (cont'd) Figure : Comparison of Methylene Selectivity App D 9 App D 8 App D 7 App D 6 μm Proteo 9 Å Zorbax 5 μm SB-C8 Å Vydac 5 μm MS5 Å Vydac 5 μm TP5 Å Selectivity to mprove Resolution Proteo can be used to resolve peptides of MW, Da, and often is able to separate peptides of only - amino acid difference. Figure compares the resolution of five peptide standards with amino acid sequences that differ in hydrophobicity by one methyl group each. Comparing the differences with respect to efficiency, selectivity, and resolution allows us to monitor the overall column performance; Proteo fully resolves each peptide. Sharper peaks with greater resolution are obtained with Proteo as compared to the competition. Methylene Selectivity Conditions for all columns: 5 x.6 mm A). % TFA B).85 % TFA in Acetonitrile Gradient: A/B (95:5) to A/B (55:5) in minutes ml/min Temperature: C Detection: nm Sample:. NH -Arg-Gly-Gly-Ala-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide. Ac-Arg-Gly-Gly-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide. Ac-Arg-Gly-Ala-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide. Ac-Arg-Gly-Val-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide 5. Ac-Arg-Gly-Val-Val-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide Easily Monitor Protein Degradation The shelf life of a protein product is a major concern in the biotechnology industry as it may be subject to inactivation from deamination or oxidation. Oxidation is commonly seen with methionine due to its readily oxidized sulfur group. A tryptic digest of ß-Lactoglobulin reveals early eluting peaks of a more polar peak representing the oxidation product. Deamidation of asparagine forms aspartic acid which is less polar and elutes slightly later in a chromatographic profile. Monitoring protein oxidation and deamidation peaks within the jungle of a tryptic digest can be difficult without adequate resolution, thus Proteo was engineered with one goal: high resolution. Columns: μm Proteo 9 Å 5 x.6 mm G-96-E A). % TFA in Water B). % TFA in Acetonitrile Gradient: A/B (95:5) for 5 min, then to A/B (6:) in 55 minutes ml/min Temperature: C Detection: nm Sample: Top Chromatogram β-lactoglobulin tryptic digest Bottom Chromatogram Oxidized β-lactoglobulin tryptic digest Oxidation of β-lactoglobulin App D 9 Control Oxidation Product App D 95 Phenomenex 9

8 Proteo (cont'd) Oligonucleotides-Purification and Analysis on Proteo Molecular biology techniques and high-throughput assays require synthesized oligonucleotides that are highly purified. Proteo provides excellent separation of oligonucleotides such that single base differences can be resolved. This excellent resolution of oligonucleotides can be used to assess purity or can be used to purify oligonucleotides from other failed sequence contaminants generated during oligonucleotide synthesis. App D 6-8mer Poly-dT Oligonucleotide Mix Column: μm Proteo 9 Å Dimension: 5 x.6 mm B-96-E A). M TEAA ph 7. B) Acetonitrile Gradient: A/B (97: ) to A/B (85:5) in min ml/min Temperature: 6 C Detection: 6 nm 5 Capillary Columns High efficiency. and.5 mm D glass-lined columns High sensitivity for low volume and mass limited samples Packed with identical materials as analytical columns for easy method transfer Consistent performance under LC/MS compatible and universal gradient conditions /8 inch ( mm) OD tube for robust columns and durability Capillary-LC columns packed with and Proteo materials provide the same characteristics as their respective analytical sized columns, but with added sensitivity for microliter sample volumes. Our high purity silica combined with proprietary bonding techniques produce dense bonded phase coverage to shield against silanol interactions. With little secondary chromatographic effects capillary-lc columns yield terrific peak shape and are compatible with reduced TFA concentration and other mass spec compatible mobile phase modifiers. App D 6 mau min mer Synthetic Oligonucleotide Purification Column: μm Proteo 9 Å Dimension: 5 x.6 mm B-96-E A). M TEAA ph 7. B) Acetonitrile Gradient: A/B (97: ) to A/B (85:5) in min ml/min Temperature: 6 C Detection: 6 nm App D 6 α-chymotrypsin Tryptic Digest on Proteo Column: μm Proteo 9 Å 5 x.5 mm F-96-AF A:. % TFA in Water B:.8 % TFA in Acetonitrile Gradient: A/B (95:5) for 5 min then A/B (55:5) in 5 min Switch to A/B (95:5) in sec then hold for min 5 µl/min Temperature: C Detection: nm Sample: α - Chymotrypsin tryptic digest 8 6 min mau 6 8 min 5 Phenomenex

9 Prep/Process Columns and 5 μm material with identical technology and base silica as 5 μm analytical products High mechanical strength with improved surface smoothness and particle sphericity High loadability with uniform, consistent pore structure High ph stability for easier column cleaning uses identical bonding and base silica technology in both analytical and preparative materials. Accordingly, 5 μm C8 material used in the analytical separation is available in a μm or 5 μm version so you can easily scale-up with minimal changes to the separation. Less Material Required materials have extremely low particle densities; meaning less material is required to pack a given volume. For large-scale preparative system this can have an enormous impact on longterm operating costs. Excellent Lifetime The excellent silica and bonding technology at the heart of provides excellent lifetimes for improved overall economy. is stable over a wide ph range (.5 to ) allowing for easier column cleaning for added column lifetime. Method Validation and Method Development Kits Since many biochromatographers work with a variety of proteins and peptides, Phenomenex has made column selection easier with the Method Development Kit. The kit provides you with one each 5 x.6 mm C, C5 and C8 columns so you may screen your sample on several phases and choose the column with the best selectivity and resolution. Method reproducibility is easily confirmed with the Method Validation Kit. These kits provide three columns from three different material batches. Kits are available with 5 μm C8 or C materials in a 5 x.6 mm column. Which Phase is right for your application Phase Application C C5 C8 Proteo (C) For proteins and polypeptides >, MW For highly hydrophobic proteins For proteins and polypeptides >, MW For highly hydrophobic proteins More retentive than C, offering slightly alternative selectivity For proteins and polypeptides >, MW For small hydrophilic proteins For separation of oligonucleotides >mer For peptides <, MW, nsulin For peptide mapping & protein digests For separation of oligonucleotides <mer ORDERNG NFORMATON Method Development Kits Part No. Description unit Price KH-98 Bioseparations kit Method Development Kit Contains one each (5 x.6 mm) 5 μm C, C5, & C8 columns KHO-77 Bioseparations kit Method Development Kit Contains one each (5 x.6 mm) 5 μm C5, C8, & μm Proteo Method Validation Kits Part No. Description unit Price KH-55 5 μm C Method Validation Kit /pk 5 x.6 mm KH-5 5 μm C5 Method Validation Kit /pk 5 x.6 mm KH-5 5 μm C8 Method Validation Kit /pk 5 x.6 mm See pp. -5 for Column Heaters. Phenomenex 5

10 ORDERNG NFORMATON μm & 5 μm Capillary Columns (mm) 5 x. 5 x. 5 x. 5 x.5 5 x.5 5 x.5 5μm C Å B-67-AC F-67-AC G-67-AC B-67-AF F-67-AF G-67-AF 5μm C8 Å B-5-AC F-5-AC G-5-AC B-5-AF F-5-AF G-5-AF μm Proteo 9 Å B-96-AC F-96-AC G-96-AC B-96-AF F-96-AF G-96-AF f does not provide you with at least an equivalent separation as compared to a column of similar phase, particle size and dimension, send in your comparative data within 5 days and keep the column for FREE. SecurityGuard Analytical Cartridges require universal holder KJO-8 μm & 5 μm Microbore and Minibore Columns (mm) SecurityGuard Cartridges 5 x. 5 x. 5 x. 5 x. 5 x. 5 x. x. mm* 5 μm C Å B-67-A F-67-A G-67-A B-67-B F-67-B G-67-B /pk AJ-9 5 μm C5 Å B-5-A F-5-A G-5-A B-5-B F-5-B G-5-B AJ-6 5 μm C8 Å B-5-A F-5-A G-5-A B-5-B F-5-B G-5-B AJ- μm Proteo 9 Å B-96-A F-96-A G-96-A B-96-B F-96-B G-96-B AJO-67 for D:.-. mm μm & 5 μm Analytical and Preparative Columns (mm) SecurityGuard Cartridges x.6 5 x.6 5 x.6 5 x.6 5 x 5 x 5 5 x. x. mm* x mm /pk /pk 5 μm C Å A-67-E B-67-E F-67-E G-67-E G-67-N G-67-AK G-67-P AJO- AJO-75 5 μm C5 Å A-5-E B-5-E F-5-E G-5-E G-5-N G-5-P AJO-7 AJO-77 5 μm C8 Å A-5-E B-5-E F-5-E G-5-E G-5-N G-5-AK G-5-P AJO- AJO-7 μm Proteo 9 Å A-96-E B-96-E F-96-E G-96-E G-96-N G-96-P AJO-67 AJO-775 for D:.-8. mm 9-6 mm μm Analytical and Preparative Columns (mm) SecurityGuard Cartridges 5 x.6 5 x 5 x 5 5 x. 5 x 5 x 5 x. mm* x mm 5 x. mm** C Å G-68-E G-68-N G-68-AK G-68-P G-68-U G-68-V /pk AJO- /pk AJO-75 /ea AJ-7 C5 Å G-5-E G-5-N G-5-P G-5-V AJO-7 AJO-77 C8 Å G-55-E G-55-N G-55-P G-55-U G-55-V AJO- AJO-7 AJ-7 Proteo 9 Å G-97-E G-97-N G-97-AK G-97-P G-97-U G-97-V AJO-67 AJO-775 AJ-78 for D:.-8. mm 9-6 mm 8- mm 5 μm Analytical and Preparative Columns (mm) SecurityGuard Cartridges 5 x.6 5 x 5 x 5 5 x. 5 x 5 x 5 x. mm* x mm 5 x. mm** C Å G-69-E G-69-N G-69-AK G-69-P G-69-U G-69-V /pk AJO- /pk AJO-75 /ea AJ-7 C5 Å G-56-E G-56-N G-56-P G-56-V AJO-7 AJO-77 C8 Å G-57-E G-57-N G-57-AK G-57-P G-57-U G-57-V AJO- AJO-7 AJ-7 Other Dimensions available upon request. for D:.-8. mm 9-6 mm 8- mm *SecurityGuard Analytical Cartridges require holder, KJ-8 Semi-prep SecurityGuard Cartridges require holder, AJ-7 **PREP SecurityGuard Cartridges require holder, AJ-8 Bulk Material ORDERNG NFORMATON μm Bulk Packings g kg 5 kg kg 5 kg kg Phase C Å G-68 K-68 L-68 M-68 N-68 P-68 C5 Å G-5 K-5 L-5 M-5 N-5 P-5 C8 Å G-55 K-55 L-55 M-55 N-55 P-55 Proteo 9 Å G-97 K-97 L-97 M-97 N-97 P-97 5 μm Bulk Packings g kg 5 kg kg 5 kg kg C Å G-69 K-69 L-69 M-69 N-69 P-69 C5 Å G-56 K-56 L-56 M-56 N-56 P-56 C8 Å G-57 K-57 L-57 M-57 N-57 P-57 See p. 8 for Fused Silica Capillary Adapter and Capillary Guard Columns. See p. 5 for Column Chiller/Heater System (8-7 C). Effectively desalt acidic, basic, and neutral peptides with strata-x. See p. 9 for more information. See p. 9 for SecurityGuard Cartridge Holders and Cartridges. 5 Phenomenex