TECHNOLOGY TRANSFER / COMMERCIALIZATION OF ICAR- CIFA TECHNOLOGY THROUGH INDUSTRIES/ ENTREPRENEURS

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1 Agri-Business Incubation Center P.O. Kausalyaganga: Bhubaneswar Tel. Nos , FAX: e.mail, Web site: TECHNOLOGY TRANSFER / COMMERCIALIZATION OF ICAR- CIFA TECHNOLOGY THROUGH INDUSTRIES/ ENTREPRENEURS, Kausalyagnaga, Bhubaneswar, Ministry of Agriculture, Government of India, is interested in technology transfer/ commercialization on non-exclusive basis through Industries/ Entrepreneurs in respect of following technology/ design developed by the institute on terms & conditions as given below. Name of the Technology/Design A. Diagnostic KITs I. Spot agglutination Kits for diagnostic of Bacterial gill disease in fish II. Dot Elisa Kits for diagnostic of Bacterial gill disease, columnaris disease in fish B. Immuno diagnostic Aids I. Rohu Catla Hybrid Detection Kit II. Argulus (A. siamensis & A. japonicas) Detection Kit III. White Tail Disease Diagnostic Kit IV. Koi Herpes Virus (KHV) Diagnostic Kit V. Spring Viraemia of Carp Diagnostic Kit C. Aquaculture Applications I. FRP Demand Fish Feeder Firms/entrepreneurs interested in the technology/design listed above are requested to fill the Expression of Interest (EOI) as per the format given in Annexure 1 and send their documents complete in all respect along with processing fee of Rs 1000/- in favor of I.C.A.R. Unit, CIFA payable at Bhubaneswar to the following address: The Director ICAR - CENTRAL INSTITUTE OF FRESHWATER AQUACULTURE, (ISO 9001:2008 Certified) Kausalyagnaga, Bhubaneswar , Odisha, India Phone: , Fax: Web :

2 Agri-Business Incubation Center P.O. Kausalyaganga: Bhubaneswar Tel. Nos , FAX: e.mail, Web site: Objectives of Commercialization: Technology leaflet attached for each technology. Terms and Conditions for application 1. The industry willing to take the technology shall be required to enter into a Memorandum of Understanding (MoU) with, Bhubaneswar, Odisha in the prescribed format, as per the terms and conditions approved by the competent authority. 2. An expert committee will scrutinize the applications for follow-up action. Shortlisted agencies/individuals will be called for a presentation regarding their strengths and business proposals. 3. Technical Commercial Committee (TCC) formed for the purpose reserves the right of rejecting any offer without assigning reasons. 4. A pre-commercialization meeting will be held at ABI-CIFA, Bhubaneswar as per request of the applicant in order to appraise regarding this technology, anticipated volume of business and any queries raised by the applicants. The parties should attend the meeting at their own cost. 5. The Agency should have/ will procure all necessary approval from different departments for manufacturing or establishing the said technology. 6. Intellectual Property Right (IPR) will remain with ICAR, New Delhi. 7. Technologies commercialization process is handled by ABI and detailed business plans are available to the potential entrepreneurs for establishment of the technology based business enterprises. 8. Information relating to the commercialization along with technology brief of the eight technologies will be available in the ICAR-CIFA website round the year. 9. The cost of the technology to be paid to ICAR-CIFA will be charged per technology per year basis. 10. The entrepreneurs/farmers/government departments will be encouraged to take technology for 3-7 years period. Minimum period will be necessarily 3 years. 11. All eight technologies will be commercialized in a non-exclusive basis. However exclusivity may be given for a particular zone with predefined terms and conditions.

3 Agri-Business Incubation Center P.O. Kausalyaganga: Bhubaneswar Tel. Nos , FAX: e.mail, Web site: There will be Four zones of exclusivity: Zone-1 (Northern zone): J&K, Punjab, Uttar Pradesh, Uttarakhand, Rajasthan, Delhi, Haryana and Himachal Pradesh Zone-2 (eastern and Northeastern Zone): North East States (8), Odisha, West Bengal and Bihar. Zone-3 (Central and Western Zone); Madhya Pradesh, Chhattisgarh, Jharkhand, Gujarat, Goa and Maharashtra Zone-4 (Southern Zone): Karnataka, Kerala, Tamil Nadu, Andhra Pradesh and Telangana 13. Each interested party would take up any zone with maximum of two zones. 14. In the zone where private person has taken the technology, there shall be a locking period of two years in which no other private parties will be given the technology. Based on the performance of the first party in each zone, the second party will be created only after two years. 15. However, in the same zone the government agencies will be allowed to take the technology irrespective of the presence of private partners. 16. The cost of diagnostics can be reduced by clubbing multiple technologies as recommended in earlier meetings. Hence, the commercial partners will be encouraged to undertake multiple numbers of diagnostic technologies to make diagnostic centers more commercially viable. 17. Applicant may choose induction by ABI-CIFA as Incubatee for a period of two years from Signing of MoU. 18. Along with the technology (like control, protocol, methods etc), the inventor scientist will also extent handholding support for the period of 6 months to establish laboratories and business enterprises. 19. Any dispute, controversy or claim arising out of or in relation to the MoU, or the breach, termination or invalidity thereof, shall be settled amicably by negotiation between the Parties. In case of failure of negotiations an arbitrator will be appointed by Director-General, Indian Council of Agricultural Research (ICAR), New Delhi. The decision of ICAR shall be final and binding. Jurisdiction for settlement of any dispute under this agreement will be Bhubaneswar. Who should apply Interested parties with credible experience, knowledge & technical capability are encouraged to apply.

4 Important Dates:- Agri-Business Incubation Center P.O. Kausalyaganga: Bhubaneswar Tel. Nos , FAX: e.mail, Web site: Submission of EoI- Open round the year Technical presentation/ discussion by innovators:- within 5 days of Submission of EoI Issue of Acceptance letter- 7 days of Submission of EoI Signing MoU After receiving full payment and within 7 days of Issue of Acceptance letter Transfer of Technology including protocols, business plans, training, literature, control etc- 90 days of signing of MoU. For detailed information of design/ technology, please see Technology Leaflet attached. For more information please visit our website: For further enquiries, please contact: The Director ICAR - CENTRAL INSTITUTE OF FRESHWATER AQUACULTURE, (ISO 9001:2008 Certified) Kausalyagnaga, Bhubaneswar , Odisha, India Phone: , Fax: Web : The Principal Investigator, Agribusiness Incubation Center (ABI) ICAR - CENTRAL INSTITUTE OF FRESHWATER AQUACULTURE, (ISO 9001:2008 Certified) Kausalyagnaga, Bhubaneswar , Odisha, India -cifaabi@gmail.com

5 Agri-Business Incubation Center P.O. Kausalyaganga: Bhubaneswar Tel. Nos , FAX: e.mail, Web site: Annexure I Proforma for submitting the Expression of Interest 1. Name of the Technology applied for 2. Name of the firm/organization/company and nationality with all contact details 3. Details of registration as an industry/ legal entity (copy of certificate to be attached) 4. State full names and addresses of persons having direct financial interest (applicable for proprietary and partnership firm) 5. Details of the manufacturing experience, qualifications, technical capabilities and expertise of the party to show that it can handle the following parameters; Production capacity, plan & space available for the proposed production work 7. Financial background with a latest copy of audited annual report attached 8. List of major customers 9. ISO certification, if any 10. Initial technology transfer fee, the party proposes to pay 11. Any other information to substitute technical and financial competence of the party

6 Agri-Business Incubation Center P.O. Kausalyaganga: Bhubaneswar Tel. Nos , FAX: e.mail, Web site: (Separate sheets may be enclosed if the space provided is insufficient; Application with incorrect and incomplete information will not be considered) I/We request that I/We may be considered for granting technical know-how for...(technology). I/We assure that all the information provided by me/us on this form is true to best of my/our knowledge, and on the basis of this information any decision taken by ICAR- Central Institute in Freshwater Aquaculture shall be accepted by me/us. I/We also undertake to furnish any further information required in this connection. Please find enclosed a Demand Draft (non-refundable)/ Bankers Cheque No. dated of Rs1000/- drawn in favor of ICAR UNIT-CIFA, Payable at Bhubaneswar, as application processing fee. 1. Application processing fee: Rs. 1000/- for Indian entities 2. Duly filled EOI application must be enclosed with the Demand draft/ banker s cheque for application processing fee drawn in favour of ''ICAR UNIT-CIFA'', Payable at Bhubaneswar, and must be sent to the Institute's address.. Signature of the Applicant Complete Postal address Technology Leaflets Attached separately

7 Agri-Business Incubation Center P.O. Kausalyaganga: Bhubaneswar Tel. Nos , FAX: e.mail, Web site: EXPRESSION OF INTEREST No. 1/1/2017 COMMERCIALIZATION OF 'EIGHT TECHNOLOGIES THROUGH INDUSTRIES/ ENTREPRENEURS The (CIFA), Kausalyaganga, Bhubaneswar, Odisha, , has developed eight commercialisable technologies. A. Diagnostic KITs I. Spot agglutination Kits for diagnostic of Bacterial gill disease in fish II. Dot Elisa Kits for diagnostic of Bacterial gill disease, columnaris disease in fish B. Immuno diagnostic Aids i. Rohu Catla Hybrid Detection Kit ii. Argulus (A. siamensis & A. japonicas) Detection Kit iii. White Tail Disease Diagnostic Kit iv. Koi Herpes Virus (KHV) Diagnostic Kit v. Spring Viraemia of Carp Diagnostic Kit C. Aquaculture Applications i. FRP Demand Fish Feeder These technologies have received appreciation from different stakeholders in widely tested field trials. To promote localized production/ manufacture to ensure the availability of this technology to the farming community, Expression of Interest (EOI) is invited from firms/ agencies/ entrepreneurs interested in commercial manufacture of this technology. The licensing will be on non-exclusive basis for a period of 3 years, with one-time payment of license fee (as indicated in each technology Leaflet) besides service tax at the prevailing rate is applicable to the license fee. Interested firms/ agencies/ entrepreneurs may also submit a brief profile of their activity, turn-over and a copy of audited financial statement of the last three years, along with a non-refundable demand draft of Rs. 1000/- drawn in favour of ICAR- CIFA, payable at Bhubaneswar towards participation fee. The EOI should be sent in a sealed envelope addressed to The Director, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar , Odisha, India Phone: , FAX: director.cifa@icar.gov.in, Website: superscribing 'EOI for technology commercialization. Principal Investigator, ABI-CIFA

8 Rohu Catla Hybrid Detection Kit Inventor Scientists: P. Jayasankar, M. Mohanty, L. Sahoo, P. Das, B.K. Das, P. Routray and D.K. Varma A PCR based identification kit has been developed which can identify hybrid in just two steps with genomic DNA as starting material. This is a specific kit designed for the identification of Labeo rohita (Rohu), Catla catla (Catla) and their Hybrid in the early life stages. This technology would really be of great use for those producers and buyers for screening of quality seeds and moreover it would be a stepping stone for seed certification programs for both government and private hatcheries. BENEFITS PCR and agarose gel based detection kit Highly specific for rohu catla hybrids in reciprocal crosses Ensure purity of seed Potential for seed certification No sequencing is required Detection can be done easily in an agarose gel Less time consuming process, 4-6 hours This technology will be of great use in the seed certification programme and will also be beneficial to the farmers for quality and pure seed culture. A state or regional level Diagnostic Center can be established by public organization and private entrepreneurs. ICAR-CIFA will provide training, handholding support and control to run this technology. Physical Requirements Financial Requirements Sample Test (in nos.) 2,000 Machinery and Equipment 6.15 Shed & Building 10'x15' Working Capital (in Lakhs) 4.29 Manpower Required Turnover (in Lakhs) Supervisor 01 no. Net Profit (in Lakhs) 3.40 Skilled Worker 01 no. IRR 23% Unskilled Worker 01 no. DSCR 1.17 Technology Cost Rs. 43,112/- per Year

9 FRP Demand Fish Feeder Inventor Scientists : B. C. Mohapatra, B. Sarkar, S. K. Singh, D. Majhi & N. Sarangi FRP demand fish feeder has main components of 10 liter capacity feed hopper and activated mechanism. The feeder is installed in a pond with the activating mechanism extending into the water through an activator rod. The feed drops by gravity onto the adjustable acrylic feed platform positioned below the hopper and above the water level. When fish activates the rod, feed pellets retained on the feed platform slowly drop on to the water surface. Benefits It is easy to construct, low cost and maintenance free. The feeder is suitable for pelleted fed and can be used in any kind of rearing system. The growth rates of fish were estimated 10-15% higher in the demand feeding cisterns / tanks fitted with feeders than the hand feeding tanks. The demand feeders will increase feed consumption in fish by making it available to them continuously. The designed feeder can hold 10 kg feed, which is sufficient to feed 500 kg fish if 2% of their body weight per day. By reducing the feed quantity to the pond, the organic pollution will be less in the feeder fitted pond. This technology will be of great use in reducing labour and also will be beneficial to the farmers by increasing efficiency of feed distribution. A state of the art manufacturing facility will be required by the entrepreneurs. ICAR-CIFA will provide training, handholding support and control to run this technology. Physical Milestone Financial Milestone Feeder Sell(in nos.) 200 Machinery and Equipment 1.25 Shed & Building 10'x15' Working Capital (in Lakhs) Turnover (in Lakhs) Manpower Required Net Profit (in Lakhs) 5.42 Skilled Worker 01 no. IRR 18% Unskilled Worker 01 no. DSCR 1.03 Technology Cost Rs. 60,144/-

10 Argulus (A. siamensis & A. japonicus) Detection Kit Inventor Scientists : P. K. Sahoo & Team In Indian aquaculture, Argulosis caused by Argulus sp., predominantly A. siamensis and A. japonicus, is one of the most devastating and economically important disease of freshwater culture system. A loss to the tune of Rs.300 crore per annum has been estimated due to this disease in India. Rapid detection and identification of this pathogen is of paramount significance for timely implementation of effective counter measures. Argulosis management measures should be species-specific for far-reaching results because both species of Argulus demonstrate differences in infection and pathology patterns. The Argulus Detection Kit utilizes the polymerase chain reaction as the method in species-specific identification of the two important Argulus sp., A. siamensis and A. japonicus. Benifits Ready to use Results comes within 8 hours only Greater accuracy compared to microscopic identification Quality assurance provided by controls DNA fragment of both species This technology will be of great use in detection of Argulus sp. and also will be economically beneficial to the farmers. A state or regional level Diagnostic Center can be established by public organization and/or private entrepreneurs. ICAR-CIFA will provide training, handholding support and control to run this technology. Physical Milestone Financial Milestone Sample Test (in nos.) 2,000 Machinery and Equipment 6.15 Shed & Building 10'x15' Working Capital (in Lakhs) 5.18 Manpower Required Turnover (in Lakhs) Supervisor 01 no. Net Profit (in Lakhs) 3.47 Skilled Worker 01 no. IRR 20% Unskilled Worker 01 no. DSCR 1.10 Technology Cost Rs. 42,948/-

11 White Tail Disease Diagnostic Kit Inventor Scientists: P. K. Sahoo & Team White Tail Disease (WTD) or White Muscle Disease (WMD) has caused mass mortality in larval and early juvenile stages as well as devastating production loss in culture systems of Macrobrachium rosenbergii (scampi) in many countries, particularly India, China, Thailand and Taiwan. Recently, it has been reported from Australia also. Benefits Significantly reduce the risk of crop failure. Conventional diagnostics fails to detect early stages of infection, hence developing a genome based detection system of this M rogenbergii nodavirus, the causative agent of WTD is essential This kit based on nested PCR method for RNA segment II is a sensitive and specific diagnostic tool that can detect carriers and early or latent infections. Detects up to four virus particles. Follows nested PCR principles High sensitivity & specificity of virus detection. The entire procedure can be completed within a day. This technology will be of great use in detection of WTD and also will be economically beneficial to the farmers. A state or regional level Diagnostic Center can be established by public organization and/or private entrepreneurs. ICAR-CIFA will provide training, handholding support and control to run this technology. Physical Milestone Financial Milestone Sample Test (in nos.) 2,000 Machinery and Equipment 6.15 Shed & Building 10'x15' Working Capital (in Lakhs) 5.18 Manpower Required Turnover (in Lakhs) Supervisor 01 no. Net Profit (in Lakhs) 3.47 Skilled Worker 01 no. IRR 20% Unskilled Worker 01 no. DSCR 1.10 Technology Cost Rs. 42,948/-

12 Koi Herpes Virus (KHV) Diagnostic Kit Inventor Scientists : P. K. Sahoo & Team The koi herpes virus is also known as cyprinid herpes virus 3 (CyHV3), belonging to the family Alloherpesviridae and widely distributed in different geographical regions around the globe. The outbreak of the virus causes mass mortality among populations of koi Cyprinus carpio koi and common carp Cyprinus carpio carpio, with clinical signs such as swelling and necrosis of gill filaments, sunken eyes and excessive mucous production leading to discolouration of skin. The ready to use PCR kit contains the components for polymerase chain reaction that allows rapid amplification of KHV DNA using the gene specific primers. The nested pcr kit can detect up to single virus particle in tissues. This kit can be used in disease surveillance, developing control measures and detection of virus in hatcheries and farms. Benefits Ready to use Time saving and cost effective Easy and rapid protocol Can be completed within 7 hours Highly specific and sensitive Nested PCR can detect even up to single purified virus particle The primers can also be used in real time PCR This technology will be of great use in detection of Koi Herpes Virus and also will be economically beneficial to the farmers. A state or regional level Diagnostic Center can be established by public organization and/or private entrepreneurs. ICAR-CIFA will provide training, handholding support and control to run this technology. Physical Milestone Financial Milestone Sample Test (in nos.) 2,000 Machinery and Equipment 6.15 Shed & Building 10'x15' Working Capital (in Lakhs) 5.18 Manpower Required Turnover (in Lakhs) Supervisor 01 no. Net Profit (in Lakhs) 3.47 Skilled Worker 01 no. IRR 20% Unskilled Worker 01 no. DSCR 1.10 Technology Cost Rs. 42,948/-

13 Spring Viraemia of Carp Diagnostic Kit Inventor Scientists : P. K. Sahoo & Team The spring viraemia of carp (SVC) is a fatal rhabdovirus infection in several carp and ictalurid fish species capable of causing an acute haemorrhagic and contagious viraemia that mainly attack the kidney, gill, spleen and encephalon. The causative pathogen is a single, non segmented RNA virus responsible for huge economic losses in various countries. For easy detection of pathogen, the CIFA developed nested RT-PCR based diagnostic kit is found to be advantageous for rapid and sensitive detection of the virus in fish. This PCR based approach can be used for routine diagnosis of the disease in field and laboratory conditions. This kit can be used in disease surveillance, developing control measures and detection of virus in hatcheries and farms. The primers provided have also been checked in real time PCR to increase the sensitivity of the kit as well as to quantify viral particles in infected tissues. Benefits Ready to use Time saving and cost effective Easy and rapid protocol Can be completed within 7 hours Highly specific and sensitive Nested PCR can detect even up to single purified virus particle The primers can also be used in real time PCR This technology will be of great use in detection of Spring Viremia and also will be economically beneficial to the farmers. A state or regional level Diagnostic Center can be established by public organization and/or private entrepreneurs. ICAR-CIFA will provide training, handholding support and control to run this technology. Physical Milestone Financial Milestone Sample Test (in nos.) 2,000 Machinery and Equipment 6.15 Shed & Building 10'x15' Working Capital (in Lakhs) Manpower Required Turnover (in Lakhs) Supervisor 01 no. Net Profit (in Lakhs) 5.95 Skilled Worker 01 no. IRR 22% Unskilled Worker 01 no. DSCR 1.15 Technology Cost Rs. 61,784/-

14 Dot-ELISA Kit Inventor Scientist : P. Swain Dot-ELISA kit is used for diagnosis of Edwardsiellosis, Aeromoniasis and Bacterial gill disease of carps. Benefits It detects the bacterial antigens of Aeromonas hydrophila, Edwardsiella tarda, Pseudomonas fluorescens and Flavobacterium branchiophilum in infected tissues upto 72 hours of death and antibodies in serum for disease diagnosis and routine health monitoring of fish stock. It is suitable for farm site and laboratory use as well as health certification by diagnostic laboratories. This technology will be of great use in detection of bacterial antigens and will be beneficial to the farmers for quality and pure fish culture. A state or regional level Diagnostic Center can be established by public organization and/or private entrepreneurs. ICAR-CIFA will provide training, handholding support and control to run this technology. Physical Milestone Financial Milestone Kit Sell (in nos.) 1000 Machinery and Equipment 2.30 Shed & Building 10'x15' Working Capital (in Lakhs) 1.55 Turnover (in Lakhs) Manpower Required Net Profit (in Lakhs) 5.88 Skilled Worker 01 no. IRR 152% Unskilled Worker 01 no. DSCR 5.50 Technology Cost Rs. 67,500/-

15 Spot Agglutination Kit Inventor Scientist : P. Swain Spot agglutination kit is an on farm quick diagnostic test, which detects the above bacterial diseases of fish such as Aeromoniasis, Edwardsiellosis, Pseudomoniasis and Bacterial gill disease in few seconds. It may be used by carp hatcheries and fish farmers to screen these specific diseases. Benefits Fast method for disease detection and samples can be tested within an hour. Easy to be used by common fish farmers. Very cheap, to be used by an ordinary fish farmers. Suitable for farm level diagnosis. Can be procured by any common fish farmer This technology will be of great use in the seed certification program and also will be beneficial to the farmers for quality and pure seed culture. A state or regional level Diagnostic Center can be established by public organization and private entrepreneurs. ICAR- CIFA will provide training, handholding support and control to run this technology. Physical Milestone Financial Milestone Kit Sell (in nos.) 2000 Machinery and Equipment 2.50 Shed & Building 10'x15' Working Capital (in Lakhs) 1.39 Turnover (in Lakhs) 9.64 Manpower Required Net Profit (in Lakhs) 5.48 Skilled Worker 01 no. IRR 140% Unskilled Worker 01 no. DSCR 5.08 Technology Cost Rs. 63,600/-

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