SOX2 antibody - Embryonic Stem Cell Marker (ab15830) datasheet
|
|
- Melina Ellis
- 6 years ago
- Views:
Transcription
1 -Embryonic Stem Cell Marker (ab15830) 1/8 ページ d Products: Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells SOX2 antibody - Embryonic Stem Cell Marker (ab15830) datasheet Product Name Product type Description Immunogen Reacts with Predicted to react (species key) Tested applications (see key) SOX2 antibody - Embryonic Stem Cell Marker Primary antibodies Rabbit polyclonal to SOX2 - Embryonic Stem Cell Marker Synthetic peptide conjugated to KLH derived from within residues of Human SOX2. (Peptide available as ab15831.) Hu, Ms, Rat, Chk, Ql, Zfsh, Dfsh Pfsh, Shp, Xl (Due to sequence homology) ChIP, ICC/IF, IHC-Fr, IHC-P, WB Abreviews Reviews by Abcam customers (see key) Read about Abreviews (Read all Abreviews) Submit an Abreview and earn up to 220 Abpoints by reviewing this product ChIP 1 Abreview - average rating: ICC/IF 2 Abreviews - average rating: IHC-P 6 Abreviews - average rating: IHC-Fr 1 Abreview - average rating: WB 2 Abreviews - average rating: IHC-P (read Abreview) IHC-P (read Abreview) IHC-P (read Abreview) IHC-P (read Abreview) Application notes (see key) Recommended dilutions ChIP: Use at a concentration of 4 袖 g/ml. ICC/IF: Use at a concentration of 3 袖 g/ml. IHC-P: 1/100-1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. IHC-Fr: Use at an assay dependent dilution. WB: Use at a concentration of 0.3 袖 g/ml. Detects a band of approximately 37 kda (predicted molecular weight: 34 kda). Positive control (see definition) Cellular localization Research areas Relevance Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. ICC: human ES cells (H9 line) WB: mouse or human embryonic stem cell lysate, Mouse Embryonic Germ Ce lysate Nuclear Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells Stem Cells >> Embryonic Stem Cells >> Intracellular Neuroscience >> Growth & Development >> Neurogenesis Nuclear Signaling >> Transcription >> Domain Families >> HMG Box Neuroscience >> Growth & Development >> Signal Transduction Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zippe >> Leucine Zipper Neuroscience >> Neuronal Markers >> Neural Stem Cells SOX 2 is also known as SRY related HMG BOX gene 2. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA
2 -Embryonic Stem Cell Marker (ab15830) 2/8 ページ d helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX1, SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse. These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution. Database links Entrez Gene (Chicken) 6657 (Human) (Mouse) (Zebrafish) SwissProt P48430 (Chicken) P48431 (Human) P48432 (Mouse) P54231 (Sheep) GeneCard Omim GC03P (Human) (Human) Unigene 601 (Chicken) 816 (Human) 4541 (Mouse) Raised in Clonality Isotype Purity Storage buffer Form Concentration Storage instructions Rabbit Polyclonal IgG Immunogen affinity purified Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, ph 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS Liquid Batch dependent within range: mg/ml Aliquot and store at -20 属 C or -80 属 C. Avoid repeated freeze / thaw cycles. At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SOX2 antibody - Embryonic Stem Cell Marker is on this datasheet. But please do contact us if you would like any reassurance! See below for SOX2 antibody - Embryonic Stem Cell Marker images, references, products related to ab15830 and other tools. SOX2 antibody - Embryonic Stem Cell Marker images: Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
3 -Embryonic Stem Cell Marker (ab15830) 3/8 ページ d Sox2-specific antibody, ab15830, stained the nucleus of human ES cells (H9 line). Upon differentiation into neurones ab15830, but not Oct4-specific antibody, stained the nuclei (data not shown). Mouse embryonic fibroblasts and ES cells differentiated into early mesoderm were not stained by ab Dilution used: 5µg/ml. Ludovic Vallier, University of Cambridge - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
4 -Embryonic Stem Cell Marker (ab15830) 4/8 ページ d Lanes 1-2 : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 1 µg/ml Lane 1: 20 µg Mouse embryonic stem cell lysate Lane 1: 20 µg Human embryonic stem cell lysate ab15830 recognised a band of the expected size (34 kda) in human and mouse embryonic stem cell lysates. Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab15830) All lanes : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 0.3 袖 g/ml Lane 1 : Mouse Embryonic Stem Cell lysate Lane 2 : Mouse Embryonic Germ Cell lysate Lane 3 : Mouse Embryonic Fibroblast Lysate Predicted band size : 34kDa Observed band size : 37 kda (why is the actual band size different from the predicted?)
5 -Embryonic Stem Cell Marker (ab15830) 5/8 ページ d Petra Hajkova, University of Cambridge Western blot All lanes : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 1 袖 g/ml Lane 1 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202) Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193) Lane 4 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) with SOX2 peptide (ab15831) at 1 袖 g/ml Lane 5 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202) with SOX2 peptide (ab15831) at 1 袖 g/ml Lane 6 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193) with SOX2 peptide (ab15831) at 1 袖 g/ml Lysates/proteins at 10 袖 l per lane. Observed band size : 42 kda (why is the actual band size different from the predicted?) A band corresponding to Sox2 was detectable in 46C Sox1-expressing Mouse Neural Progenitor Cell Lysate (ab27205), IOUD2 Oct4-expressing Mouse Embryonic Stem Cell Lysate (ab272020) and F9 Mouse Embryonic Carcinoma Cell Lysate (ab27193). This band was detected in the lysate by Western Blotting using anti-sox2 antibody ab The Sox2 bands were specifically blocked using the immunising peptide of ab An Oct4 band was also detectable in the 47C lysate by Western Blotting (data not shown). It has not been possible to find a suitable Sox1-specific antibody able to detect Sox1 in 46C by Western Blotting. However, we are confident that 100% of the cells (excluding feeders) that contributed to the lysate were actively expressing Sox1. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
6 -Embryonic Stem Cell Marker (ab15830) 6/8 ページ d SOX2 antibody - Embryonic Stem Cell Marker (ab15830) on Formalin/PFA-fixed paraffin-embedded adult zebra fish spinal cord sections. Antigen retrieval step: Heat mediated. 1%BSA as blocking agent for 10 mins at RT 属 C. ab15830 used at 1/600 for 2 hours.secondary Antibody: Biotin conjugated goat anti rabbir IgG used at 1/600. Carl Hobbs, KCL, UK Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830) Immunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for SOX2 antibody - Embryonic Stem Cell Marker (ab15830) on Dogfish/Catshark Tissue sections. Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody used at 1/600 incubated for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti IgG. Carl Hobbs, CARD, KCL, London, UK
7 -Embryonic Stem Cell Marker (ab15830) 7/8 ページ d Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830) Immunohistochemistical detection (on formaldehyde/pfa-fixed paraffin-embedded sections) of SOX2 antibody - Embryonic Stem Cell Marker (ab15830) on Quail Tissue sections (brain stem). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins RT. Primary Antibody ab15830 incubated at 1/600 for 2 hours. Secondary Antibody: Biotin labelled goat anti rabbit IgG. This image shows nuclear expression in the ventricular region of the embryonic quail brain stem. Carl Hobbs, CARD, KCL, London, UK Customer reviews (feedback) regarding SOX2 antibody - Embryonic Stem Cell Marker Customer enquiries (FAQ's) regarding SOX2 antibody - Embryonic Stem Cell Marker Protocols for SOX2 antibody - Embryonic Stem Cell Marker Price and availability of products related to SOX2 antibody - Embryonic Stem Cell Marker SOX2 antibody - Embryonic Stem Cell Marker - related products: Products for ChIP ChIP Related Products Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) Slides Products for Western blot Loading controls Lysates Immunizing Peptide for SOX2 antibody - Embryonic Stem Cell Marker ab15831 SOX2 peptide Compatible Secondaries for SOX2 antibody - Embryonic Stem Cell Marker ab6564 Cy5 速 antibody ab6717 FITC antibody ab6720 Biotin antibody ab6721 HRP antibody ab6722 Alkaline Phosphatase antibody
8 -Embryonic Stem Cell Marker (ab15830) 8/8 ページ d ab6793 ab7007 ab7019 ab27234 ab27235 ab27236 ab27237 ab27238 ab27239 ab27240 ab31193 ab60314 ab60315 ab60317 ab60319 Texas Red 速 antibody Phycoerythrin antibody Agarose antibody 10nm Gold antibody 5nm Gold antibody 15nm Gold antibody 20nm Gold antibody 30nm Gold antibody 10nm Gold antibody 5nm Gold antibody Duck polyclonal to Rabbit IgG Chromeo 痰 488 antibody Chromeo 痰 494 antibody Chromeo 痰 546 antibody Chromeo 痰 642 antibody Other SOX2 Antibodies See all SOX2 antibodies (2)... All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" contact us with your comments Abcam plc. All rights reserved. Terms and Conditions Legal Privacy Policy Investor Relations Contact Us
SANTA CRUZ BIOTECHNOLOGY, INC.
TECHNICAL SERVICE GUIDE: Western Blotting 2. What size bands were expected and what size bands were detected? 3. Was the blot blank or was a dark background or non-specific bands seen? 4. Did this same
More informationProduct Datasheet. Acetylcholinesterase/ACHE Antibody (HR2) NB Unit Size: 200uL. Store at -20C. Avoid freeze-thaw cycles.
Product Datasheet Acetylcholinesterase/ACHE Antibody (HR2) NB300-528 Unit Size: 200uL Store at -20C. Avoid freeze-thaw cycles. Publications: 1 Protocols, Publications, Related Products, Reviews, Research
More informationProduct Datasheet. TGN38 Antibody NBP Unit Size: 0.5 mg
Product Datasheet TGN38 Antibody NBP1-03495 Unit Size: 0.5 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 5 Protocols, Publications, Related Products,
More informationCustom Antibodies Services. GeneCust Europe. GeneCust Europe
GeneCust Europe Laboratoire de Biotechnologie du Luxembourg S.A. 2 route de Remich L-5690 Ellange Luxembourg Tél. : +352 27620411 Fax : +352 27620412 Email : info@genecust.com Web : www.genecust.com Custom
More informationMATF Antigen Submission Details and Standard Project Deliverables
MATF Antigen Submission Details and Standard Project Deliverables What we require when you submit your antigen: Proteins For a recombinant protein target, we require a minimum of 400µg soluble recombinant
More informationGlobo H Monoclonal Antibody (VK9), ebioscience Catalog Number Product data sheet
Website: thermofisher.com/ebioscience Customer Service (US): 1-888-999-1371 thermofisher.com/contactus Globo H Monoclonal Antibody (VK9), ebioscience Catalog Number 14-9700-82 Product data sheet Details
More informationUnderstanding secondary antibodies
Understanding secondary antibodies IgG Fragment antigen binding antibodies and isotypes D2 D2 F(ab ) 2 after pepsin cleavage www.abcam.com/secondary_antibody Antibody structure and F(ab) antibodies The
More informationab TripleStain IHC Kit: M&M&R on human tissue (DAB, Red/AP & DAB/Ni)
ab183287 TripleStain IHC Kit: M&M&R on human tissue (DAB, Red/AP & DAB/Ni) Instructions for Use For the detection of Rabbit and Mouse Primary antibodies on Human tissue or cell samples. This product is
More informationab Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC Kit
ab64264 - Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC Kit Instructions for Use For the detection of a specific antibody bound to an antigen in tissue sections. This product is for research use
More informationmcherry Monoclonal Antibody (16D7) Catalog Number M11217 Product data sheet
Website: thermofisher.com Customer Service (US): 1 800 955 6288 ext. 1 Technical Support (US): 1 800 955 6288 ext. 441 mcherry Monoclonal Antibody (16D7) Catalog Number M11217 Product data sheet Details
More informationab Mouse and Rabbit AP/Fast-Red (ABC) Detection IHC Kit
ab128967 - Mouse and Rabbit AP/Fast-Red (ABC) Detection IHC Kit Instructions for Use For the detection of a specific antibody bound to an antigen in tissue sections. This product is for research use only
More informationAntibody Purification Guide
Guide Innova Biosciences Guide Innova Biosciences Ltd. Babraham Research Campus, Cambridge, UK, CB22 3AT +44 (0)1223 661000 info@innovabiosciences.com Guide 2 Innova Biosciences specializes in easy to
More information1. Cross-linking and cell harvesting
ChIP is a powerful tool that allows the specific matching of proteins or histone modifications to regions of the genome. Chromatin is isolated and antibodies to the antigen of interest are used to determine
More informationab Ran Activation Assay Kit
ab173247 Ran Activation Assay Kit Instructions for Use For the simple and fast measurement of Ran activation. This product is for research use only and is not intended for diagnostic use. Version 1 Last
More informationWesternMAX Alkaline Phosphatase Chemiluminescent Detection Kits
WesternMAX Alkaline Phosphatase Chemiluminescent Detection Kits Code N221-KIT N220-KIT Description WesternMAX Chemiluminescent AP Kit, Anti-Mouse Includes: Alkaline Phosphatase (AP) Conjugated Anti-Mouse
More informationPSC 4-Marker Immunocytochemistry Kit PSC (OCT4, SSEA4) Immunocytochemistry Kit PSC (SOX2, TRA-1-60) Immunocytochemistry Kit
PSC 4-Marker Immunocytochemistry Kit PSC (OCT4, SSEA4) Immunocytochemistry Kit PSC (SOX2, TRA-1-60) Immunocytochemistry Kit Catalog no. A24881, A25526, A25525 Table 1 Contents and storage Kit component
More informationOverview of Solulink Products. June 2011
Overview of Solulink Products June 2011 1 Who we are Established in 2003, Solulink develops, patents, manufactures, and sells consumables to over 1,000 customers in life science, diagnostic, and pharmaceutical
More informationAnti-Piscirickettsia salmonis monoclonal antibody. Product no: P05
Anti-Piscirickettsia salmonis monoclonal antibody Product no: P05 Product Description The monoclonal antibody (Mab) against Piscirickettsia salmonis is specific for this bacterium. The specificity of the
More informationOverview of Immunohistochemistry. (with a focus on wax-embedded sections)
Overview of Immunohistochemistry (with a focus on wax-embedded sections) Overview of Immunohistochemistry (with a focus on wax-embedded sections) Overview of Immunohistochemistry IHC is like cooking. There
More informationCERTIFICATE OF ANALYSIS
5171 Wilfong Road Memphis, TN 38134 Telephone: 901-382-8716 Fax: 901-333-8223 Email: info@meridianlifescience.com www.meridianlifescience.com CERTIFICATE OF ANALYSIS Important Note: Centrifuge before opening
More informationYour Antibody Source. prosci-inc.com. Extensive Antibody Services Broad Antibody Catalog
Your Source prosci-inc.com Extensive Services Broad Catalog Company Overview Established in 1998, ProSci Incorporated is a leading provider of high performance antibodies and custom antibody services.
More informationab Antibody Serum Purification Kit (Protein A) Protocol
ab109209 Antibody Serum Purification Kit (Protein A) Protocol For preparing antibodies for conjugation The components of Ab109209 are fully compatible with our Conjugation kits however they are not compatible
More informationab G alpha i Activation Assay Kit
ab173234 G alpha i Activation Assay Kit Instructions for Use For the simple and fast measurement of G alpha i activation. This product is for research use only and is not intended for diagnostic use. Version
More informationProduct Information. Before you begin. Component A 1 vial of 30 ul vial of 300 ul each Glycerol. Tris
Glowing Products for Science Mix-n-Stain Antibody Labeling Kits Size: 1 labeling per kit Storage: -20 o C Stability: Stable for at least 1 year from date of receipt when stored as recommended. Components:
More informationmcherry Polyclonal Antibody Catalog Number PA Product data sheet
Website: thermofisher.com Customer Service (US): 1 800 955 6288 ext. 1 Technical Support (US): 1 800 955 6288 ext. 441 mcherry Polyclonal Antibody Catalog Number PA5-34974 Product data sheet Details Size
More informationProduct Data Sheet - TRUEMAB
888.267.4436 techsupport@origene.com www.origene.com Name:F13A1 (Factor XIIIa) mouse monoclonal antibody, clone OTI1H2 (formerly 1H2) Product Data Sheet - TRUEMAB Catalog: TA800305 Components: F13A1 (Factor
More informationMouse Monoclonal Antibody Isotyping Reagents
Mouse Monoclonal Antibody Isotyping Reagents Catalog Number: SEK003 Storage Temperature: 2-8 C Fax : +86-10-58628220 Tel : +86-400-890-9989 http://www.sinobiological.com Description Mouse Monoclonal Antibody
More informationmcherry Rat Monoclonal Antibody
mcherry Rat Monoclonal Antibody Catalog no. M11217 Table 1 Contents and storage Material Amount Concentration Storage Stability mcherry Rat Monoclonal Antibody, unconjugated 100 μl 2 mg/ml in 1X PBS, 0.09%
More informationRabMAb advantages A guide to our rabbit monoclonal antibodies
RabMAb advantages A guide to our rabbit monoclonal antibodies Discover more at abcam.com/rabmab Table of Contents About RabMAb technology... 3 RabMAb technology vs traditional mouse monoclonal technology...
More informationCytoGLOW. IKK-α/β. Colorimetric Cell-Based ELISA Kit. Catalog #: CB5358
CytoGLOW IKK-α/β Colorimetric Cell-Based ELISA Kit Catalog #: CB5358 Please read the provided manual entirely prior to use as suggested experimental protocols may have changed. Research Purposes Only.
More informationab K-Ras Activation Assay Kit
Version 1 Last updated 7 July 2016 ab211159 K-Ras Activation Assay Kit For the rapid, sensitive and accurate measurement of active K-Ras in cell or tissue lysates from human, mouse or rat. This product
More informationProduct Datasheet. Panendothelial Cell Antigen Antibody (MECA-32) NB Unit Size: 0.5 mg
Product Datasheet Panendothelial Cell Antigen Antibody (MECA-32) NB100-77668 Unit Size: 0.5 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 14 Protocols,
More informationProduct Datasheet. mcherry Antibody (1C51) NBP Unit Size: 0.1 ml
Product Datasheet mcherry Antibody (1C51) NBP1-96752 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 1 Publications: 18 Protocols, Publications,
More informationProduct Datasheet. SOD2/Mn-SOD Antibody NB Unit Size: 0.05 mg. Store at -20C. Avoid freeze-thaw cycles. Publications: 19
Product Datasheet SOD2/Mn-SOD Antibody NB100-1992 Unit Size: 0.05 mg Store at -20C. Avoid freeze-thaw cycles. Publications: 19 Protocols, Publications, Related Products, Reviews, Research Tools and Images
More informationRat IGF-1 ELISA Kit (rigf-1-elisa)
Rat IGF-1 ELISA Kit (rigf-1-elisa) Cat. No. EK0377 96 Tests in 8 x 12 divisible strips Background Insulin-like growth factor 1 (IGF-1), also known as somatomedin C, is a polypeptide protein hormone similar
More informationImmunohistochemistry. How does it look like? When do we need IHC? When do we need IHC? In clinic: In research:
Introduction How does it look like? Immunohistochemistry Smooth muscle actin Parvalbumin Distrophyn Sandrine Bichet Head of Molecular Histology Platform Signal versus background 06.03.2012 IHC basics Introduction
More informationImmunoprecipitation Protocol
Immunoprecipitation Protocol Immunoprecipitation is a general method to obtain the enrichment of a specific protein from tissue lysate and cell lysate. It can be used to purify a specific protein, to identify
More informationspecial offers from your protein biology resource
special offers from your protein biology resource Pop open your cells, extract your proteins, purify, quantify and express them. Seeking knowledge about proteins with Thermo Scientific Protein Research
More informationAntibodies to Animal Cells and Serum Proteins
ANTIBODIES TO ANIMAL CELLS AND SERUM PROTEINS Antibodies to Animal Cells and Serum Proteins Antibodies to Animal Cellular Antigens Antibodies to Mouse Brain Antibodies to Animal Serum Proteins INTERNATIONAL
More informationSpecies predicted to react based on 100% sequence homology: Chicken, Bovine, Dog.
1 of 5 11/1/2013 10:25 PM Product Pathways - Jak/Stat Pathway Phospho-Stat3 (Tyr705) Antibody #9131 Have you tried your application using our XP monoclonal antibodies? Try products: 9145 PhosphoSitePlus
More informationOne-Step Western TM Kit using TMB
Technical Manual No. 0203 Version 03272008 I Description.. 1 II Kit Contents.. 2 III Applications 3 IV Key Features.. 3 V Storage.. 3 VI One-Step Western TM Protocol. 3 VII Examples. 3 VIII Troubleshooting..
More informationApplication Note AN001
Testing hybridoma supernatants with the Spots On Dots Antibody Screening Kit Application Note AN1 Table of Contents Overview... 2 Figure 1. Screening of hybridomas raised against peptide antigens... 3
More informationIMMUNOPRECIPITATION TROUBLESHOOTING TIPS
IMMUNOPRECIPITATION TROUBLESHOOTING TIPS Creative Diagnostics Abstract Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds
More informationINOS. Colorimetric Cell-Based ELISA Kit. Catalog #: OKAG00807
INOS Colorimetric Cell-Based ELISA Kit Catalog #: OKAG00807 Please read the provided manual entirely prior to use as suggested experimental protocols may have changed. Research Purposes Only. Not Intended
More informationStrep-tag detection in Western blots
Strep-tag detection in Western blots General protocol for the detection of Strep-tag fusion proteins Last date of revision April 2012 Version PR07-0010 www.strep-tag.com For research use only Important
More informationCell & Tissue Staining Kit
Cell & Tissue Staining Kit For the detection of goat, mouse, rabbit, rat, or sheep primary IgG Antibodies Size: 50 Tests HRP-DAB System Goat Kit (Catalog Number CTS008) Mouse Kit (Catalog Number CTS002)
More informationIn-Cell ELISA. Validated antibodies, dyes and kits for the analysis of fixed adherent and suspension cells. Discover more at abcam.
In-Cell ELISA Validated antibodies, dyes and kits for the analysis of fixed adherent and suspension cells. In-Cell ELISA Advantages of In-Cell ELISA Flexible Measure specific protein level or post-translation
More informationUpdated PRODUCT INSERT. HPI Catalog # HP5-XXX
Updated 2017 1 PRODUCT INSERT Hypoxyprobe, Inc. 121 Middlesex Turnpike Burlington, MA 01803 USA www.hypoxyprobe.com Hypoxyprobe Gemini Kit HPI Catalog # HP5-XXX Kit Contents: Solid pimonidazole HCl (Hypoxyprobe
More informationEGFR (Phospho-Ser695)
Assay Biotechnology Company www.assaybiotech.com Tel: 1-877-883-7988 Fax: 1-877-610-9758 EGFR (Phospho-Ser695) Colorimetric Cell-Based ELISA Kit Catalog #: OKAG02090 Please read the provided manual entirely
More informationOptiblot ECL Ultra Detect Kit (1.2pg 2ng)
ab133409 Optiblot ECL Ultra Detect Kit (1.2pg 2ng) Instructions for Use For enhanced chemiluminescent Western blotting. This product is for research use only and is not intended for diagnostic use. 1 Table
More informationab SUMOylation Assay Kit
ab139470 SUMOylation Assay Kit Instructions for Use For the generation and detection of SUMOylated proteins in vitro. This product is for research use only and is not intended for diagnostic use. Version
More informationManufactured by. Zyagen Barnes Canyon Road San Diego, CA 92121, USA
Alkaline Phosphatase Immunohistochemistry Detection kits For detection of mouse, rabbit, goat, rat, sheep, chicken, guinea pig, and human primary antibodies Size: 500 Tests Catalog #: AK-011, Mouse Kit
More informationab Ubiquitylation Assay Kit
ab139467 Ubiquitylation Assay Kit Instructions for Use For the activation of ubiquitin for use in ubiquitylation experiments This product is for research use only and is not intended for diagnostic use.
More informationImmunological Techniques in Research and Clinical Medicine. Philip L. Cohen, M.D. Chief of Rheumatology, LKSOM 10 March 2016
Immunological Techniques in Research and Clinical Medicine Philip L. Cohen, M.D. Chief of Rheumatology, LKSOM 10 March 2016 Antibodies Remarkable Tools for Research and Diagnosis You can make an antibody
More informationHow to run Alpha assay: How to setup an Alpha assay Make your own assay!
How to run Alpha assay: How to setup an Alpha assay Make your own assay! 1 2009 PerkinElmer AlphaLISA kits - recommendations before starting the assay Samples: Phenol red and hemoglobin: choose AlphaLISA
More informationp53 Monoclonal Antibody (PAb 122) Catalog Number MA Product data sheet
Website: thermofisher.com Customer Service (US): 1 800 955 6288 ext. 1 Technical Support (US): 1 800 955 6288 ext. 441 p53 (PAb 122) Catalog Number MA5-12453 Product data sheet Details Size 500 µl Host/Isotope
More informationKinase Reaction and Alkylation Protocol
Kinase Reaction and Alkylation Protocol Protocol for the treatment of substrates prior to detection by Thiophosphate Ester antibodies This product is for research use only and is not intended for diagnostic
More informationab Ubiquitylation Assay Kit (HeLa lysate-based)
ab139471 Ubiquitylation Assay Kit (HeLa lysate-based) Instructions for Use For the generation of ubiquitin-conjugated lysate proteins This product is for research use only and is not intended for diagnostic
More informationCF Dyes Next Generation Fluorescent Dyes Secondary antibody
CF Dyes Next Generation Fluorescent Dyes Secondary antibody OZYME 10 AVENUE AMPÈRE - CS 30268-78053 ST QUENTIN EN YVELINES CEDEX Tél. : 01 34 60 24 24 - Fax : 01 34 60 92 12 - www.ozyme.fr/info CF Dyes
More informationab Optiblot Fluorescent Western Blot Kit
ab133410 Optiblot Fluorescent Western Blot Kit Instructions for Use For quantitative, multi-color fluorescent Western blotting. This product is for research use only and is not intended for diagnostic
More informationA Bridging Immunogenicity Assay Using SPARCL TM Technology
A Bridging Immunogenicity Assay Using SPARCL TM Technology Wenhua F. Xie Lumigen Inc., a Beckman Coulter Company INTRODUCTION Evaluating the immune response in patients is an important aspect associated
More informationAnti-Asian Sea bass (Lates calcarifer) IgM monoclonal antibody labelled with horseradish peroxidase. Product no: C2-HRP
Anti-Asian Sea bass (Lates calcarifer) IgM monoclonal antibody labelled with horseradish peroxidase Product no: C2-HRP Product Description This monoclonal antibody (Mab) reacts with Asian Sea bass (Lates
More informationab Alkaline Phosphatase Conjugation Kit Protocol
ab102850 Alkaline Phosphatase Conjugation Kit Protocol Antibody and protein modification This product is for research use only and is not intended for diagnostic use. Version 2 Last Updated 12 March 2014
More informationphab Amine and Thiol Reactive Dyes for Antibody Internalization Studies Nidhi Nath, Ph.D. Group Leader, Protein Analysis Promega Corporation
phab Amine and Thiol Reactive Dyes for Antibody Internalization Studies Nidhi Nath, Ph.D. Group Leader, Protein Analysis 1 Outline 1. phab Dyes 2. Protocols for conjugating phab Dyes to antibodies 3. Applications:
More informationFlow Cytometry - The Essentials
Flow Cytometry - The Essentials Pocket Guide to Flow Cytometry: 1. Know your Cytometer 2. Understanding Fluorescence and Fluorophores 3. Gating Process 4. Controls 5. Optimization 6. Panel Building 7.
More informationLSBio TM Mouse/Human/Rat CAMK2B / CaMKII Beta Cell-Based ELISA Kit. Catalog No. LS-F1847. User Manual
LSBio TM Mouse/Human/Rat CAMK2B / CaMKII Beta Cell-Based ELISA Kit Catalog No. LS-F1847 User Manual Please Read the Manual Carefully Before Starting your Experiment For research use only. Not approved
More informationAntibody Services from GenScript
Services from GenScript www.genscript.com GenScript USA Inc. 860 Centennial Ave., Piscataway, NJ 08854 USA Toll-Free: 1-877-436-7274 Fax: 1-732-210-0262 1-732-855-5878 Academic Services Pharmaceutical
More informationRayBio Human NF-κB p65 Transcription Factor Activity Assay Kit
RayBio Human NF-κB p65 Transcription Factor Activity Assay Kit Catalog #: TFEH-p65 User Manual Mar 13, 2017 3607 Parkway Lane, Suite 200 Norcross, GA 30092 Tel: 1-888-494-8555 (Toll Free) or 770-729-2992,
More informationPurification Kits. Fast and Convenient PROSEP -A and PROSEP-G Spin Column Kits for Antibody Purification DATA SHEET
 Montage Antibody Purification Kits Fast and Convenient PROSEP -A and PROSEP-G Spin Column Kits for Antibody Purification DATA SHEET Available with immobilized Protein A or Protein G Easy-to-use Antibody
More informationTechnical Note Detection of post-immunoprecipitation proteins by Western blot using the Quick Western Kit IRDye 680RD
Technical Note Detection of post-immunoprecipitation proteins by Western blot using the Quick Western Kit IRDye 680RD Developed for: Aerius, Odyssey Classic, Odyssey CLx and Odyssey Sa Imaging Systems
More informationOptiblot. Western Blot Reagents and Troubleshooting Tips. Discover more at abcam.com/optiblot
432_12_GM Optiblot booklet02_layout 1 28/08/2012 15:14 Page 1 Optiblot Western Blot Reagents and Troubleshooting Tips High quality protein electrophoresis and western blot reagents Our Optiblot range of
More informationOrexin A (HUMAN, MOUSE, RAT, PORCINE, OVINE,
Orexin A (HUMAN, MOUSE, RAT, PORCINE, OVINE, BOVINE) Western Blot Kit Protocol (Catalog #WBK-003-30) PHOENIX PHARMACEUTICALS, INC. TABLE OF CONTENTS 1. Kit Contents...2 2. Storage...2 3. Introduction...3
More informationRabbit (monoclonal) Anti-FAK [py 397 ] Phosphospecific Antibody, Unconjugated
Rabbit (monoclonal) Anti-FAK [py 397 ] Phosphospecific Antibody, Unconjugated PRODUCT ANALYSIS SHEET Catalog Number: Lot Number: Volume: 44-625G (10 mini-blot size) See product label 100 μl Clone Number:
More informationImmunohistochemistry: Basics and Methods
Immunohistochemistry: Basics and Methods Bearbeitet von Igor B Buchwalow, Werner Böcker 1st Edition. 2010. Buch. x, 153 S. Hardcover ISBN 978 3 642 04608 7 Format (B x L): 15,5 x 23,5 cm Gewicht: 445 g
More informationEdU Flow Cytometry Kit. User Manual
User Manual Ordering information: (for detailed kit content see Table 2) EdU Flow Cytometry Kits for 50 assays: Product number EdU Used fluorescent dye BCK-FC488-50 10 mg 6-FAM Azide BCK-FC555-50 10 mg
More informationTECHNICAL BULLETIN. MEK Activity Assay Kit. Product Code CS0490 Storage Temperature 20 C
MEK Activity Assay Kit Product Code CS0490 Storage Temperature 20 C TECHNICAL BULLETIN Product Description The MAP kinase kinases (MAPKK, mitogen-activated protein kinase kinase, also termed MEK) are a
More informationMicroarray Industry Products
Via Nicaragua, 12-14 00040 Pomezia (Roma) Phone: +39 06 91601628 Fax: +39 06 91612477 info@lifelinelab.com www.lifelinelab.com Microarray Industry Products Page 10 NBT / BCPIP Chromogenic phosphatase
More informationElectrophoresis and transfer
Electrophoresis and transfer Electrophoresis Cation = positively charged ion, it moves toward the cathode (-) Anion = negatively charged ion, it moves toward the anode (+) Amphoteric substance = can have
More informationConjugation Kit Protocol
ab102890 HRP Conjugation Kit Protocol Antibody and protein modification This product is for research use only and is not intended for diagnostic use. Version 5 Last Updated 1 July 2016 Table of Contents
More informationDiscovery and Humanization of Novel High Affinity Neutralizing Monoclonal Antibodies to Human IL-17A
Discovery and Humanization of Novel High Affinity Neutralizing Monoclonal Antibodies to Human IL-17A Contacts: Marty Simonetti martysimonetti@gmail.com Kirby Alton kirby.alton@abeomecorp.com Rick Shimkets
More informationPhospho-STAT3 (Ser727) Polyclonal Antibody Catalog Number
Website: thermofisher.com Customer Service (US): 1 800 955 6288 ext. 1 Technical Support (US): 1 800 955 6288 ext. 441 Phospho-STAT3 (Ser727) Polyclonal Antibody Catalog Number 44-384G Product data sheet
More informationNEW Streptavidin-PE Conjugates Streptavidin-HRP Conjugates Streptavidin-AP Conjugates 7 + 8
High Quality Stable Liquid Substrates, Conjugates and Stabilizing Diluents for Immunoassays, Flow Cytometry and Multiplexing Platforms such as Luminex Substrates Page Chemiluminescent HRP for ELISA and
More informationHEK 293 Host Cell Proteins
HEK 293 Host Cell Proteins Immunoenzymetric Assay for the Measurement of HEK 293 Host Cell Proteins Catalog # F650R Intended Use This kit is intended for use in determining the presence host cell protein
More informationab Mouse Antibody Purification Kit Protocol
ab128745 Mouse Antibody Purification Kit Protocol For preparing antibodies for conjugation The components of ab128745 are fully compatible with our Conjugation kits however they are not compatible with
More informationProduct Datasheet. YAP1 Antibody (2F12) H M01. Unit Size: 0.1 mg. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Product Datasheet YAP1 Antibody (2F12) H00010413-M01 Unit Size: 0.1 mg Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Publications: 30 Protocols, Publications, Related Products, Reviews,
More information1. Paraffin section slides can be stored at room temperature for a long time.
Immunohistochemistry (IHC) Protocols Immunohistochemistry (IHC) Protocol of Paraffin Section 1. Fix dissected tissues with 10% formalin for no less than 48 hours at room temperature. Inadequately fixation
More informationXfect Protein Transfection Reagent
Xfect Protein Transfection Reagent Mammalian Expression Systems Rapid, high-efficiency, low-toxicity protein transfection Transfect a large amount of active protein Virtually no cytotoxicity, unlike lipofection
More informationRayBio Phospho- Stat 3 (Tyr705) ELISA Kit
RayBio Phospho- Stat 3 (Tyr705) ELISA Kit For Measuring Phosphorylated Stat3 (Tyr705) in Human, Mouse and Rat Cell Lysates User Manual (Revised Mar 1, 2012) RayBio Stat3 (Tyr705) ELISA Kit Protocol (Cat#:
More informationHuman Pluripotent Stem Cell Functional Identification Kit
Human Pluripotent Stem Cell Functional Identification Kit Catalog Number SC027B Reagents for the identification of human pluripotent stem cells by in vitro functional differentiation. This package insert
More informationIn-Cell Western Kits I and II
Odyssey and Aerius Infrared Imaging Systems In-Cell Western Assay Kits I and II Published November, 2006. The most recent version of this protocol is posted at http://biosupport.licor.com/protocols.jsp
More informationSegments of the obstructed intestinal loops were fixed in 4% paraformaldehyde
Supplementary text Supplementary materials and methods Histopathological examination Segments of the obstructed intestinal loops were fixed in 4% paraformaldehyde (PFA) and embedded in paraffin wax with
More informationRayBio Phospho- Akt (Ser473) ELISA Kit
RayBio Phospho- Akt (Ser473) ELISA Kit For Measuring Phosphorylated Akt (Ser473) in Human, Mouse and Rat Cell Lysates User Manual (Revised Mar 1, 2012) RayBio Akt (Ser473) ELISA Kit Protocol (Cat#: PEL-Akt-S473-001)
More informationGenScript. Neuroscience Products & Services. Your Innovation Partner in Drug Discovery! Tools for Neurological Disease Research:
Neuroscience Products & Services Reliable, well-selected antibodies are one of the most effective tools in the neuroscience researcher's arsenal with regard to identifying and locating specific proteins.
More informationMyers Lab ChIP-seq Protocol v Modified January 10, 2014
Myers Lab ChIP-seq Protocol V011014 1 Contact information: Dr. Florencia Pauli Behn HudsonAlpha Institute for Biotechnology 601 Genome Way Huntsville, AL 35806 Telephone: 256-327-5229 Email: fpauli@hudsonalpha.org
More informationELISPOT and FLUOROSPOT kits
ELISPOT and FLUOROSPOT kits Interleukins Interferons Granzymes and perforins TNF superfamily ligands and receptors Apoptosis markers And many more... ELISPOT and FLUOROSPOT: a cell-based assay to assess
More informationChinese Hamster Ovary Host Cell Proteins
Chinese Hamster Ovary Host Cell Proteins Immunoenzymetric Assay for the Measurement of Chinese Hamster Ovary Host Cell Proteins Catalog # F015 Intended Use This kit is intended for use in determining the
More informationPARP-1 (cleaved) Human In-Cell ELISA Kit (IR)
ab110215 PARP-1 (cleaved) Human In-Cell ELISA Kit (IR) Instructions for Use For the quantitative measurement of Human PARP-1 (cleaved) concentrations in cultured adherent and suspension cells. This product
More informationAcetyl-p53 (K381) Cell-Based Colorimetric ELISA Kit
Acetyl-p53 (K381) Cell-Based Colorimetric ELISA Kit Catalog No. KA8015C Detection and Quantification of Acetyl-p53 (K381) Protein Concentration in Cell. Research Purposes Only. Not Intended for Diagnostic
More informationMRC5 Host Cell Proteins
MRC5 Host Cell Proteins Immunoenzymetric Assay for the Measurement of MRC5 Host Cell Proteins Catalog # F300 Intended Use This kit is intended for use in determining the presence of MRC5 host cell protein
More informationSupplemental Data. LMO4 Controls the Balance between Excitatory. and Inhibitory Spinal V2 Interneurons
Neuron, Volume 61 Supplemental Data LMO4 Controls the Balance between Excitatory and Inhibitory Spinal V2 Interneurons Kaumudi Joshi, Seunghee Lee, Bora Lee, Jae W. Lee, and Soo-Kyung Lee Supplemental
More information