Globo H Monoclonal Antibody (VK9), ebioscience Catalog Number Product data sheet

Transcription

1 Website: thermofisher.com/ebioscience Customer Service (US): thermofisher.com/contactus Globo H Monoclonal Antibody (VK9), ebioscience Catalog Number Product data sheet Details Size 100 µg Host/Isotope Mouse / IgG3, kappa Class Monoclonal Type Antibody Clone VK9 Conjugate Unconjugated Form Liquid Concentration 0.5 mg/ml Purification Affinity chromatography Storage buffer PBS, ph 7.2 Contains 0.09% sodium azide Storage Conditions 4 C Species Reactivity Tested species reactivity Published species reactivity Human Tested Applications Dilution * Flow Cytometry (Flow) Immunohistochemistry (Paraffin) (IHC (P)) Western Blot (WB) Published Applications Miscellaneous PubMed (MISC) Flow Cytometry (Flow) Human, Not Applicable Assay-Dependent 5 µg/ml 5 µg/ml See 4 publications below See 1 publications below * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls. Product specific information Description: The VK9 monoclonal antibody recognizes human Globo H, a hexasaccharide formed from the pentasaccharide precursor, SSEA3 (stagespecific embryonic antigen 3). It is a member of a family of antigenic carbohydrates expressed on the surface of cancer cells as glycolipids and possibly glycoproteins. Globo H is overexpressed on many carcinomas, such as breast, colon, ovarian, gastric, pancreatic, lung, and prostate. Expression is observed in more than half of all ductal, lobular, and tubular breast carcinomas, but not in non-epithelial breast tumors. Globo H is also expressed on cancer stem cells and human embryonic stem cells, although expression decreases when cells are differentiated toward both neural progenitor cells and to a lesser extent in endodermal lineage cells. Expression in normal tissues is limited to cells composing secretory borders. Globo H is considered a target for immunotherapy of many epithelial cancers. Applications Reported: This VK9 antibody has been reported for use in flow cytometric analysis, western blotting, immunohistochemical staining of formalinfixed paraffin embedded tissue sections, and microscopy. Applications Tested: This VK9 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using either high or low ph antigen retrieval at less than or equal to 5 ug/ml. The VK9 antibody has also been tested by western blot of reduced MCF-7 cell lysate and can be used at less than or equal to 5 ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. Background/Target Information Globo H is a hexasaccharide formed from the pentasaccharide precursor, SSEA3 (stage-specific embryonic antigen 3). It is a member of a family of antigenic carbohydrates expressed on the surface of cancer cells as glycolipids and possibly glycoproteins. Globo H is overexpressed on many carcinomas, such as breast, colon, ovarian, gastric, pancreatic, lung, and prostate. Expression is observed in more than half of all ductal, lobular, and tubular breast carcinomas, but not in non-epithelial breast tumors. Globo H is also expressed on cancer stem cells and human embryonic stem cells, although expression decreases when cells are differentiated toward both neural progenitor cells and to a lesser extent in endodermal lineage cells. Expression in normal tissues is limited to cells composing secretory borders. Globo H is considered a target for immunotherapy of many epithelial cancers.

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3 Product Images For Globo H Monoclonal Antibody (VK9), ebioscience Globo H Antibody ( ) Immunohistochemistry of formalin-fixed paraffin embedded human breast cancer tissue stained with 5 µg/ml of Mouse IgG3 K Isotype Control Purified (left) or 5 µg/ml of Anti-Human Globo H Purified (right) followed by Anti- Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.nuclei are counterstained with hematoxylin.

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5 PubMed References For Globo H Monoclonal Antibody (VK9), ebioscience 4 Miscellaneous PubMed References Species / Dilution 1 Flow Cytometry References Species / Dilution Summary Stem cells (Dayton, Ohio) (Dec 2011; 29: 1995) "Changes in glycosphingolipid composition during differentiation of human embryonic stem cells to ectodermal or endodermal lineages." Author(s):Liang YJ,Yang BC,Chen JM,Lin YH,Huang CL,Cheng YY,Hsu CY,Khoo KH,Shen CN,Yu J PubMed Article URL: Published figure using Globo H monoclonal antibody (Product # ) in Miscellaneous Proceedings of the National Academy of Sciences of the United States of America (Aug 2008; 105: 11667) "Expression of Globo H and SSEA3 in breast cancer stem cells and the involvement of fucosyl transferases 1 and 2 in Globo H synthesis." Author(s):Chang WW,Lee CH,Lee P,Lin J,Hsu CW,Hung JT,Lin JJ,Yu JC,Shao LE,Yu J,Wong CH,Yu AL PubMed Article URL: Proceedings of the National Academy of Sciences of the United States of America (Aug 2008; 105: 11661) "Glycan microarray of Globo H and related structures for quantitative analysis of breast cancer." Author(s):Wang CC,Huang YL,Ren CT,Lin CW,Hung JT,Yu JC,Yu AL,Wu CY,Wong CH PubMed Article URL: Glycoconjugate journal (Mar 1998; 15: 243) "Characterization of a mouse monoclonal IgG3 antibody to the tumor-associated globo H structure produced by immunization with a synthetic glycoconjugate." Author(s):Kudryashov V,Ragupathi G,Kim IJ,Breimer ME,Danishefsky SJ,Livingston PO,Lloyd KO PubMed Article URL: Summary Published figure using Globo H monoclonal antibody (Product # ) in Miscellaneous Proceedings of the National Academy of Sciences of the United States of America (Aug 2008; 105: 11667) "Expression of Globo H and SSEA3 in breast cancer stem cells and the involvement of fucosyl transferases 1 and 2 in Globo H synthesis." Author(s):Chang WW,Lee CH,Lee P,Lin J,Hsu CW,Hung JT,Lin JJ,Yu JC,Shao LE,Yu J,Wong CH,Yu AL PubMed Article URL: