Product Datasheet. mcherry Antibody (1C51) NBP Unit Size: 0.1 ml

Transcription

1 Product Datasheet mcherry Antibody (1C51) NBP Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 1 Publications: 18 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 6/11/2017 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NBP mcherry Antibody (1C51) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer Target Molecular Weight Product Description Host Species Specificity/Sensitivity Immunogen Product Application Details Applications Recommended Dilutions Application Notes 0.1 ml 1 mg/ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Monoclonal 1C51 10mM Sodium Azide IgG2a Protein G purified PBS 28 kda Mouse All Species Does not cross react with GFP. Page 1 of 4 v.20.1 Updated 6/11/2017 This antibody is raised against recombinant full-length mcherry with His tag purified from E. coli. Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry Western Blot 1:2000, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry 1:500 This mcherry (1C51) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 28 kda. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID ). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID ). Use in Immunoprecipitation reported in scientific literature (PMID ).

3 Images Immunocytochemistry/Immunofluorescence: mcherry Antibody (1C51) [NBP ] - shows Hek293 cells transfected with mcherry and visualized in red. The cells were stained with NBP in the green channel, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mcherry and NBP Untransfected Hek293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue). Page 2 of 4 v.20.1 Updated 6/11/2017 Western Blot: mcherry Antibody (1C51) [NBP ] - WB assay of the crude extract of Hek293 cells transfected with pfin-ef1-mcherry vector (lane +) and an equal amount of protein extract from untransfected Hek293 cells (lane -). NBP binds a major band running at about 28kDa corresponding to intact full-length mcherry. The two other bands are clearly processed forms of mcherry as they are not present in nontransfected Hek293 cells.

4 Publications Pan D, Klare K, Petrovic A et al. CDK-regulated dimerization of M18BP1 on a Mis18 hexamer is necessary for CENP- A loading. Elife Jan 6 [PMID: ] (WB, Human) Pan D, Klare K, Petrovic A et al. CDK-regulated dimerization of M18BP1 on a Mis18 hexamer is necessary for CENP- A loading Elife 2017 Jan 6 [PMID: ] (WB, Human) Rebman JK, Kirchoff KE, Walsh GS. Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons. PLoS ONE Oct :00AM [PMID: ] (IHC-P, Zebrafish) Li X, Rydzewski N, Hider A et al. A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation. Nat Cell Biol 2016 Apr [PMID: ] (WB) Cho A, Kato M, Whitwam T et al. An Atypical Tropomyosin in Drosophila with Intermediate Filament-like Properties Cell Rep 2016 Jul 26 [PMID: ] (IHC) Estruch SB, Graham SA, Deriziotis P, Fisher SE. The language-related transcription factor FOXP2 is posttranslationally modified with small ubiquitin-like modifiers. Sci Rep Feb 12 [PMID: ] (WB, Human) Han B, Tiwari A, Kenworthy AK. Tagging strategies strongly affect the fate of overexpressed caveolin-1. Traffic 2015 Apr [PMID: ] Ruas M, Davis LC, Chen CC et al. Expression of Ca2+-permeable two-pore channels rescues NAADP signalling in TPC-deficient cells EMBO J Apr 14 [PMID: ] (ICC/IF, Mouse) Liang Hongqing, Esposito Alessandro, De Siddharth et al. Homeostatic control of polo-like kinase-1 engenders nongenetic heterogeneity in G2 checkpoint fidelity and timing. Nat Commun Jun 04 [PMID: ] (WB) Zhu M, Li X, Tian X, Wu C. Mask loss-of-function rescues mitochondrial impairment and muscle degeneration of Drosophila pink1 and parkin mutants Hum. Mol. Genet Mar 05 [PMID: ] (WB) Details: Mouse anti-mcherry antibody (clone 1C51) used for WB analysis of mcherry-atg8 levels in larval muscles expressing 24B-Gal4-driven UAS-mCherry-ATG8 together with the control RNAi, the mask RNAi or UAS-Mask, at 93 h after egg laying. The antibody was used at 1 : 1000 dilution and the data image shows free mcherry as well as ATG 8 bound mcherry (Figure 6B). Yousef H, Morgenthaler A, Schlesinger C et al. Age-Associated Increase in BMP Signaling inhibits Hippocampal Neurogenesis. Stem Cells Dec 23 [PMID: ] Andrews OE,Cha DJ,Wei C, Patton JG. RNAi-Mediated Gene silencing in Zebrafish Triggered by Convergent Transcription Sci Rep Jun 09 [PMID: ] (WB) Details: mcherry antibody (clone 1C51) used in WB - At 72?hpf, GFP+ embryos were de-yolked and protein lysates were prepared from 30 embryos in lysis buffer. 25?ug of total protein was loaded per lane, Lanes 1 and 2 correspond to UIC embryos from 2 separate clutches, Lane 3 (empty), Lane 4: CT-mCherry injected embryos, Lane 5: CT-mCherry + 2.5ng Dicer MO, Lane 6: non-ct mcherry injected embryos. Alpha-tubulin was detected at 50 kda whereas the band at ~27kDa corresponds to mcherry (Supplemental Figure 1; cropped image in Figure 2C ). More publications at Page 3 of 4 v.20.1 Updated 6/11/2017

5 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NBP HAF007 NB720-B NBP Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] Mouse IgG2a Isotype Control NBP G mcherry Antibody (1C51) [DyLight 488] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: