Biochemical Markers. Antibodies & recombinant proteins. Biochemical messengers Tumour markers Cardiovascular Immunology Metabolic Cerebral FABPs

Transcription

1 Biochemical Markers Antibodies & recombinant proteins Biochemical messengers Tumour markers Cardiovascular Immunology Metabolic Cerebral FABPs

2 Randox Life Sciences is recognised as a primary manufacturer of high quality, cost-effective human recombinant proteins, polyclonal and monoclonal antibodies, which can be used for a wide variety of research and development applications. Randox Life Sciences products are manufactured at our ISO certified manufacturing plant in the United Kingdom using state of the art facilities. Our product range includes over 200 antibodies and over 100 human recombinant proteins, including both classical and novel markers. The quality systems within Randox Life Sciences are monitored regularly both internally and externally enabling us to provide reliable and consistent products of the highest quality. Biochemical Markers Over the last 40 years biochemistry has become exceedingly successful at explaining living processes that now almost all areas of the life sciences from botany to medicine are engaged in biochemical research. Today the main focus for many researchers and developers is in understanding how biological molecules give rise to the processes that occur within living cells, which in turn relates greatly to the study and understanding of whole organisms. The increased advancements in health, medicine and diagnostics over the past century can be significantly attributed to advances resulting from biochemical and clinical research. Randox Life Sciences aims to further accelerate this scientific discovery by developing and marketing beneficial and unique products in the forefront of life science. With a large investment in research and development along with over 200 R&D scientists our portfolio is constantly expanding to provide you with an increased choice of high quality cost effective reagents. Our products cover research areas such as Cancer, Cardiovascular, Immunology, Neuroscience, Metabolic and Biochemical Messengers and includes a growing range of non-primary antibody products such as Human Recombinant Proteins, corresponding HRP labelled Conjugates and ScFv s.

3 Antibodies Our range of antibodies offers the following benefits: Excellent sensitivity High concentration Excellent specificity to target compound Detailed cross reactivity profiles Available for bulk purchase Stable when stored at -20 o C Excellent reproducibility between lot numbers. General polygonal specification Host Animal: Sheep Format: Ig fraction prepared by caprylic acid and ammonium sulphate precipitation ph: 7.2 Buffer: 20mM Phosphate, 150mM Sodium Chloride Preservative: 0.09% Sodium Azide Storage: All antibodies can be stored at 2-8 C or frozen at -20 C General monoclonal specification Host Animal: Sheep Format: Purified high affinity monoclonal antibody ph: 7.2 Buffer: PBS Preservative: 0.09% Sodium Azide Method of Production: In vitro cell culture using standard DMEM media supplemented with foetal bovine serum of a USDA approved origin Method of Purification: Protein A Affinity Chromatography

4 Immunology Adhesion Molecules E-Selectin L Selectin L-Selectin (Monoclonal) ICAM-1 ICAM-1 (Monoclonal) VCAM-1 VCAM-1 (Monoclonal) Cytokines Eotaxin GM-CSF GM-CSF (Monoclonal) Interferon Gamma MCP-1 PDGF-A PDGF-B TNF-α TNF-β Visfatin Interleukins IL-1α IL-1β IL-1Ra IL-10 IL12p35 IL12p40 IL-13 IL-15 Chemokines Fractalkine Inducible Protein 10 (IP-10) MIP-1a Rantes Inflammatory TREM-1 IL-2 IL-3 IL-4 IL-6 IL-7 IL-8 Chemokines Fractalkine Inducible Protein 10 (IP-10) MIP-1a Rantes All Randox polyclonal biochemical markers are available as affinity purified Cardiovascular Apolipoprotein E4 (Monoclonal) Carbonic Anhydrase (CA III) (Muscle) Cardiac Troponin 1 (ctni) (Monoclonal) Cardiac Troponin 1 (Capture) (Monoclonal) Cardiac Troponin 1 (Detector 1) (Monoclonal) Cardiac Troponin 1 (Detector 2) (Monoclonal) FABP (Muscle and Heart) Human Transferrin ICAM-1 ICAM-1 (Monoclonal) LP-PLA2 Myoglobin (Human) Procalcitonin Tissue Factor 4

5 Cancer Cytokeratin -18 GST-01 (Mutant)(Monoclonal) GST-01 (Wild-type)(Monoclonal) Hepatocyte growth factor A Human Apolopoprotein AI Human Apolopoprotein B Human Epidermal Growth Factor (EGF) Inducible Protein 10 (IP-10) M2Pk Tumour Necrosis Factor Alpha (TNFα) Tumour Necrosis Factor B (TNFβ) VEGF VEGF-R1 Hydroxyindole Acetic Acid Biochemical Messengers Cyclic AMP Cyclin-Dependent Kinase 4 (CDK4) Cytokeratin-18 (CK18) Retinol Binding Protein S100A10 S100A11 S100B Serum Amyloid A (SAA, Human) TSH-β (Clone 1)(Monoclonal) TSH-β (Clone 2)(Monoclonal) TSH-β (Capture-Clone 3)(Monoclonal) TSH-β (Detector-Clone 4)(Monoclonal) Cerebral Apolipoprotein E4 (Monoclonal) GST-01 (Mutant)(Monoclonal) GST-01 (Wild-type)(Monoclonal) Human Apolopoprotein A1 Neuronal Specific Enolase (NSE) NGAL Human Apolopoprotein A1 TSH-β (Clone 1)(Monoclonal) TSH-β (Clone 2)(Monoclonal) TSH-β (Capture-Clone 3)(Monoclonal) TSH-β (Detector-Clone 4)(Monoclonal) Metabolic Cotinine Creatinine Creatinine (Monoclonal) Creatinine Acid Cystatin C Ethylglucuronide Ethylglucuronide (monoclonal) Human Apolopoprotein A1 Human Apolopoprotein B Human C-Reactive Protein Plasminogen activator Inhibitor (PAI-1) Resistin Fatty Acid Binding Proteins Cyclic AMP Cyclin-Dependent Kinase 4 (CDK4) Cytokeratin-18 (CK18) Retinol Binding Protein S100A10 S100A11 S100B Serum Amyloid A (SAA, Human) TSH-β (Clone 1)(Monoclonal) TSH-β (Clone 2)(Monoclonal) TSH-β (Capture-Clone 3)(Monoclonal) TSH-β (Detector-Clone 4)(Monoclonal) 5

6 Human Recombinant Proteins As well as antibodies we can also provide an extensive range of human recombinant proteins which offer the following benefits: Carrier free format presented in phosphate buffered saline or tris in 50% glucerol High purity >95% Western Blot controls available. Different pack sizes available: 10ug, 100ug and 1mg Suitable for long term storage at -20oC Evaluated by SDS-Page, Western Blot Analysis, ELISA Randox provide an extensive range of human recombinant proteins and antibodies specific to a wide range of biochemical markers including: Chemokines Like cytokines, chemokines are chemical messengers involved in the inflammatory response. Chemokines act by sending out signals attracting white blood cells to the site of disease or injury. Chemokines are also involved in many disease states including infectious disease and cancer. Tumour markers Tumour Markers can indicate that a malignant process is present and are used to screen for cancer or monitor the course of disease. Tumour Markers can be found in blood, tissue or urine. Adhesion molecules Adhesion molecules are complex membrane proteins. Adhesion Molecules are grouped into four major families: the selectins, the immunoglobulin (Ig) superfamily, integrins and cadherins. Adhesion molecules are involved in a wide range of physiological processes and can be found in the cell membrane or as soluble forms in circulation. Altered levels of these proteins have been found in pathological states including cardiovascular disease, stroke, cancer, diabetes and many others. Cytokines Cytokines are produced by many different cell types and function as intercellular chemical messengers. Cytokines help control and mediate haematopoiesis as well as immune and inflammatory processes and have been implicated in many disease states including cancer, cardiovascular disease and allergic responses. Proteases Proteases occur naturally in all organisms catalysing the cleavage of peptide bonds in proteins. These enzymes are involved in a multitude of physiological reactions from simple digestion of food proteins to highly-regulated cascades. Proteases present in blood serum play important roles in blood-clotting, as well as lysis of the clots, and the correct action of the immune system. Other proteases are present in leukocytes and play several different roles in metabolic control. 6

7 Human Recombinant Proteins Gamma A Disintegrin and Metalloproteinase Domain 12 S Isoform ADAM 10 BLA-R1 Carbonic Anhydrase III CD40-L C-Reactive Protein Cyclin-Dependant Kinase 4 Cyctatin C Eotaxin E-Selectin FABP (Adipocyte) FABP (Brain) FABP (Myelin) Fractalkine Gamma Hydroxybutyrate CoA Transferase Gluthione S- Transferase Omega A Gluthione S- Transferase Omega D Granulocyte Colony Stimulating Factor Granuclocye Macophage Colony Stimulating Factor Haptoglobin β-chain Hepatocyte Growth Factor-A Hepatocyte Growth Factor-B Inhibin A Inhibin alpha subunit Inhibin beta subunit Integrin-Linked Kinase Intracellular Adhesion Molecule (ICAM-1) Interferon Gamma Interferon Inducible Protein Interleukin-1 Alpha Interleukin-1 Receptor Antagonist Interleukin-10 Interleukin 12p35 Interleukin 12p40 Interleukin-13 Interleukin-15 Interleukin -17 Interleukin-1β Interleukin-2 Interleukin-2 Receptor Alpha Interleukin-3 Interleukin-33 Interleukin-35 Interleukin-4 Interleukin-6 Interleukin-6 Soluble Receptor Interleukin-7 Interleukin-8 Kalikrein 6 Lipoprotein-Associated Phospholipase A2 L-Selectin Macrophage Inflammatory Protein-1α Matrix Metalloproteinase-9 Migration Inhibitory Factor Mitochondrial Glycerol-3-Phosphate Dehydrogenase Monomine Oxidase B Monocyte Chemotactic Protein-1 Myoglobin Neuronal Specific Enolase Neurophil Gelatinase Associated Lipocalin Paroxnonase 1 Paroxonase 2 Penicillin Binding Protein 2X Peroxisom Proliferator-Activated Receptor Gamma Plasminogen Activator Inhibitor 1 Platelet Derived Growth Factor A (PDGF-A) Platelet Derived Growth Factor B (PDGF-B) Pregnancy Associated Plasma Protein-1 Procalcitonin Prolactin Pro-Matrix Metalloprotinase 9 P-Selectin Rantes Resistin Retinol Binding Protein S100 Beta Chain S100A10 S100A11 Soluble Triggering Receptor Expressed on Myeloid Cells -1 Soluble Tumour Necrosis Factor Receptor-1 (stnfr1) Soluble Tumour Necrosis Factor Receptor-2 stnfr2) ST-2 Tissue Factor Tissue Inhibitor of Metalloprotinase 1 Transforming Growth Factor Beta-1 Tropomysin 1 Varient 3 Tumour Necrosis Factor Alpha (TNFα) Tumour Necrosis Factor Beta (TNFβ) Tumour Type M2 Pyruvate Kinase (M2PK) Vascular Cell Adhesion Molecule-1 (VCAM-1) Vascular Cell Adhesion Molecule-1 Fragment Vascular Endothelial Growth Factor (VEGF) Vascular Endothelial Growth Factor Receptor (VEGFR) Visfatin 7

8 Research Applications Elisa Western blot analysis Dot blot analysis Flow cytometry Immunohistochemistry Immunoprecipitation Antibody recognition assay Custom Manufacturing Services Randox Life Sciences provide a broad range of bacterial protein expression services; a complete solution for your research needs which can be tailored to help you overcome your cloning and protein expression needs. Our protein expression services include: Bioinformatic analysis and domain selection Subclone plasmid/construct expression vectors Sequence analysis and verification Bacterial cell transformation and clone preparation Protein expression and purification Optimisation of expression and purification conditions Quality control report Scale up production Protein Characterisation Your project manager at Randox Life Sciences will work with you in developing the best strategy to achieve your protein expression needs whilst using the most cost effective processes available. With extensive experience our scientists have been producing human recombinant proteins that are employed in our own in house diagnostic kits. Order online Randox Life Sciences, 55 Diamond Road, Crumlin, County Antrim, BT29 4QY, United Kingdom t +44 (0) f +44 (0) e lifesciences@randox.com I Information correct at time of print. All Randox products are made in the UK. Randox Laboratories Limited is a company registered within Northern Ireland with company number N.I VAT Registered Number: GB Product availability may vary from country to country. Please contact your local Randox representative for information. LT235 OCT12