MagCore Automated Nucleic Acid Extractor Overview

Transcription

1 MagCore Automated Extractor Overview Cost -Effective Cost-Effective MagCore HF16 New Generation MagCore HF16 Plus New! Spectrophotometer Built-in MagCore Super New! Economical and Space Saving MagCore Compact High Capacity Module MagCore HF48 MagCore Automated Extractor MagCore 1-8 Samples 1-16 Samples 1-48 Samples Built-in Spectrophotometer MagCore HF48 MagCore e Super MagCore Core HF1 HF16 UI Touch Screen UV Decontamination MagCore gcore C Compact Barcode Scanner optional optional Thermo Printer optional MagCore HF16Plus Programs Bulit-in (Upgradeable via RS232 ports) MagCore ti 96 Tests MagCore oe N 36 Tests Programs Bulit-in (Upgradeable via USB ports, Plug&Play)

2 MagCore MagCore Selection Guide Genomic DNA Viral s Total RNA MagCore : MagCore Genomic DNA Whole Blood Kit (Speedy installation) MagCore Genomic DNA Whole Blood Kit MagCore Genomic DNA Large Volume Whole Blood Kit (1.2 ml) MagCore Plasma DNA Kit (1.2 ml) MagCore Cultured Cells DNA Kit MagCore Genomic DNA Plant Kit MagCore Genomic DNA Tissue Kit MagCore Genomic DNA FFPE One-Step Kit MagCore Genomic DNA Bacterial Kit MagCore Viral Kit MagCore Viral Kit (Low PCR Inhibition) MagCore Viral Large Volume Kit (2.4 ml) MagCore Viral Large Volume Kit (1.2 ml) MagCore Total RNA Whole Blood Kit MagCore Total RNA Cultured Cells Kit Cat No. Cat No. Cat No. 36 preps 72 preps 96 preps MGB MGB MCC-01 MGP-01 MGT-01 MGF-01 MGF-02 MBB-01 MVN MVN MRN-01 MRC-01 MGB MGB MGB1200 MPD1200 MCC-02 MGP-02 MGT-02 MBB-02 MVN MVN MVN2400 MVN1200 MRN-02 MRC-02 * * * For isolation of total RNA from animal tissues and FFPE samples, the MagCore total RNA Cultured Cells Kit is a relatively suitable choice and functions with a special protocol. In the near future, a more unique and convenient reagent will be developed for the extraction of RNA from animal tissue and FFPE samples.

3 MagCore For extraction of genomic DNA from human whole blood sample by using MagCore System MagCore Genomic DNA Whole Blood Kit is designed for extraction of total DNA (including genomic, mitochondrial and viral DNA) from whole blood, plasma, serum, buffy coat. The pre-filled cartridge contains proteinase K, a chaotropic salt and guanidine hydrochloride for cell lysis and protein degradation. The chaotropic salt helps the strong binding of DNA and cellulose coated magnetic beads. After the removal of contaminants, the high quality DNA is eluted by low salt elution buffer or water. Purified DNA of approximately kb in length is suitable for PCR or other enzymatic reactions MagCore Genomic DNA Whole Blood Kit (Fast installation) For 200 and 400 μl sample volumes. Contents: pre-filled cartridges (including proteinase K), disposable tip & holder sets, sample tubes, elution tubes Shelf life: 6 months MagCore Genomic DNA Whole Blood Kit For 200 and 400 μl sample volumes. Contents: pre-filled cartridges, proteinase K, PK storage buffer, disposable tip & holder sets, sample tubes, elution tubes MagCore Genomic DNA Large Volume Whole Blood Kit (1.2 ml) For 1200 μl sample volumes. Contents: pre-filled cartridge, proteinase K, PK storage buffer, disposable tip & holder sets, sample tubes, elution tubes Features 1. High performance of purified DNA in downstream applications such as qpcr. 2. Higher analytical sensitivity. 3. Pre-filled and sealed buffer cartridges prevent contamination. 4. Linear yields from a wide range of viral titers. 5. Walkaway processing enables efficient workflow. Applications The MagCore Genomic DNA Whole Blood Kit is designed to allow automated processing of multiple sample types in the same run. Sample types include: 1. Fresh and frozen whole blood 2. Buffy coat 3. Body fluids The high quality DNA obtained for many applications, such as: 1. PCR and real time PCR 2. Genotyping or sequencing 3. SNP, STR

4 Performance Different anticoagulants testing DNA quality analysis by Spectrophotometer Anticoagulant A 260 / 280 Conc.(ng/μl) 1. EDTA Sodium Citrate Lithium Heparin(4ml) Sodium Fluoride Lithium Heparin(9ml) Agarose gel electrophoresis Kbps 10 M Flexibility in sample volumes Protocols for 200 and 400 μl extraction. MagCore beads have superior binding capacity with DNA of 200 to 400 ul whole blood or buffy coat samples. DNA yields are highly increased. Purified gdna suitable for downstream applications Genomic DNA purified by the MagCore Whole Blood Genomic DNA Kit is ideal for sensitive downstream applications such as PCR, Real-Time PCR and multiple PCR. Users can count on its reliability to routinely operate genomic DNA extraction. Linear increase in DNA yield ul elution 200 ul elution Lane M: RBC 1kb DNA Ladder Marker Lane 1~6: Genomic DNA of same whole blood samples. Agarose gel electrophoresis P M Gel elelectrophoresis ectro esiso so oft target gene ec Cbl-b b( (1.7 (17 1.7Kb Kb)f followed l owedb byp PCR is to check ifan inhibitioneffect effectisinducedbydifferentant induced different ntanticoagulants anticoagulants. Lane M: RBC 1kb DNA Ladder Marker. Lane 1~5: PCR results of target gene Cbl-b (1.7 Kb) Lane P: Positive control. 5 kb 2 kb 1 kb MagCore Specification Cat.No. MGB (36 tests / kit ) / MGB (96 tests / kit ) Cartridge Code : 101 MagCore Genomic DNA Whole Blood Kit (Speedy installation) Running Time: 45 mins for 200μl samples / 56 mins for 400μl samples Cat.No. MGB (36 tests / kit ) / MGB (96 tests / kit ) Cartridge Code : 102 MagCore Genomic DNA Whole Blood Kit (36 tests / kit) Running Time: 45 mins for 200μl samples / 56 mins for 400μl samples Cat.No. MGB1200 (96 tests / kit ) Cartridge Code : 104 MagCore Genomic DNA Large Volume Whole Blood Kit (1.2 ml) Running Time: 76 mins for 1200μl samples It is shown that there is a scalability of DNA isolation from blood samples (20, 50, 80, 100, 150, and 200 μl) by the MagCore HF16 The amount of DNA was determined by A 260 measurement. Consistentyieldandpurity Yield (ug) of 0.4ml Whole Blood Yield (ug) of 0.2ml Whole Blood Purity Genomic DNA was purified from different whole blood samples (0.2 ml and 0.4ml) by MagCore. The isolated genomic DNA was eluted in 100 μl and 200 μl of elution buffer. Consistency and reproducibility of DNA purification Absorbance measurements by NanoDrop N1000. The spectrum shows the same quality DNA can be isolated from five separate whole blood samples using MagCore products

5 MagCore For extraction of free circulating DNA from human plasma or serum by using MagCore system The kit is designed for purification of free circulating DNA from human serum or plasma by performing with the MagCore instrument. The kit contains all required reagents and labware for automated purification based on magnetic-particle technology. Reagents necessary for a complete process are supplied and pre-filled in the cartridges, which can be easily loaded into the MagCore instrument. 105 MagCore Plasma DNA Kit (1.2 ml) For 1200μl sample volumes. Contents: pre-filled cartridges, proteinase K, PK storage buffer, disposable tip & holder sets, sample tubes, elution tubes. Features 1. High performance of purified DNA in downstream applications such as qpcr. 2. Efficient recovery of fragmented DNA 3. Pre-filled and sealed buffer cartridges prevent contamination. 4. No phenol or chloroform extraction 5. Efficient removal of contaminants and inhibitors. Applications The MagCore Plasma DNA Kit efficiently purifies free circulating DNA from volumes 1.2 ml. Sample types include: 1. Human plasma 2. Human serum The high quality DNA obtained is suited for many applications, such as: 1. PCR and real-time PCR 2. Next Generation Sequencing (NGS)

6 Performance High-quality of free DNA from plasma and serum Quality and quantity analysis of the free DNA Bioanalyzer by Agilent Sample ID Sample type Superior quality DNA are available from plasma and serum samples by using MagCore products. 1 Human plasma Target Conc. 35 bp marker (pg/μl) Conc. (pg/μl) bp marker Conc. (pg/μl) 75 2 Human serum High stability of freedna Quality and quantity analysis of the free DNA by Agilent 2100 Bioanalyzer. alyze It isshow shownbythee he evidence e that free DNA of high qualityand and stability is possible to extract from 5 different rentpl plasma samples by using MagCore procudts. Sample ID Sample type Target Conc. (pg/μl) 35 bp marker Conc. (pg/μl) bp marker Conc. (pg/μl) 1 Human plasma Human plasma Human plasma Human plasma Human plasma MagCore Specification Cat.No. MPD1200 (96 tests / kit ) Cartridge Code : 105 MagCore Plasma DNA Kit Running Time: 76 mins for 1200μl samples

7 MagCore For genomic DNA extraction from cultured cells and amniotic fluid by using MagCore MagCore Cultured Cells DNA Kit is designed to extract genomic DNA from up to 5x10 6 cultured cells by performing with the MagCore instrument. The kit contains all required reagents and labware for automated extraction using magnetic-particle technology. Reagents necessary for a complete process are supplied in prefilled cartridges, which can be easily loaded into the MagCore instrument. 110 MagCore Cultured Cells DNA Kit For 200μl sample volumes (up to 5 x10 6 cells) Contents: pre-filled cartridges, proteinase K, PK storage buffer, disposable tip & holder sets, sample tubes, elution tubes Features 1. High performance of purified DNA in downstream applications such as qpcr. 2. Reliable isolation of DNA up to 5 x10 6 cells 3. Pre-filled and sealed buffer cartridges prevent contamination. 4. No phenol or chloroform extraction. 5. Efficient removal of contaminants and inhibitors. Applications The high quality DNA obtained is suited for many applications, such as: 1. PCR and real-time PCR 2. Next Generation Sequencing (NGS)

8 Performance DNA quality analysis by Spectrophotometer DNA of three different cultured cells were extracted by the employment of MagCore HF16 and MagCore Cultured Cells DNA Kit (200μl sample volume). The following data show the yield and purity of DNA of three samples. Sample 1: COS 7 (1.2x10 7 ) Sample 2: Junkat (1x10 6 ) Sample 3: HEK293 (1x10 6 ) Amniotic fluid DNA extration Result DNA quality is checked by the NanoDrop spectrophotometer and agarose gel electrophoresis after DNA purification from amniotic-fluid cells. Cells were harvested from 10~15 ml amniotic fluid samples at pregnancy weeks by centrifugation for 10 minutes at 3000 rpm. Sample ID Conc. (pg/μl) A260 / 280 A260 / 230 Yield (μg) Yield (mg) DNA purity (A260/280) (1) GPT2967P (2) GPT2952P (3) NP679P (4) NP777P (5) MP795P by NanoDrop ND-1000 M Table 1. DNA quality analysis by NanoDrop. Sample ID (1) to (5) are different amniotic fluid samples. It is shown that MagCore HF16 system can purify 1~2 μg DNA from10~15 ml amniotic fluid samples and DNA purity is with the A 260/280 ratio of around 1.8±0.1 M kb MagCore Specification Cat.No. MCC-01 (36 tests / kit ) / MCC-02 (96 tests / kit ) Cartridge Code : 110 MagCore Cultured Cells DNA Kit Running Time: 44 mins for 200μl samples 1% Agarose gel electrophoresis, 110V 5 μl sample loading Figure 1. Genomic DNA isolations ions were run by gel electrophoresis on 1% agarose gel. Lane M: RBC 1kb ladder marker Lane 1: GPT2967P Lane 2: GPT2952P Lane 3: NP679P Lane 4: NP777P Lane 5: NP795P

9 MagCore For extraction of viral DNA/RNA from serum, plasma and cell-free body fluids by using MagCore system The MagCore Viral Kit is for purification of viral DNA and RNA from serum, plasma, cell-free body fluids. MagCore magnetic particle technology provides high-quality DNA/RNA that is suitable for direct use in downstream applications such as amplification or other enzymatic reactions. For a minimum risk of cross-contamination, plastic consumables inside the Kit are DNase/RNase treated and the operation system is designed to individually processes samples at the same time. Multiple protocols are installed on the instrument and optimized for different sample volumes MagCore Viral Kit For 200 and 400 μl sample volumes Contents: pre-filled cartridges, proteinase K, PK storage buffer, carrier RNA, RNase free water, disposable tip & holder sets, sample tubes, elution Tubes MagCore Viral Kit (Low PCR Inhibition) For 200 and 400 μl sample volumes Contents: pre-filled cartridges, proteinase K, PK storage buffer, carrier RNA, RNase free water, disposable tip & holder sets, sample tubes, elution tubes MagCore Viral Large Volume Kit (2.4 ml) For 2400 μl sample volumes Contents: pre-filled cartridges, proteinase K, PK storage buffer, carrier RNA, RNase free water, disposable tip & holder sets, sample tubes, elution tubes (special T-rack required for 5 ml sample tubes) MagCore Viral Large Volume Kit (1.2 ml) For 1200 μl sample volumes Contents: pre-filled cartridges, proteinase K, PK storage buffer, carrier RNA, RNase free water, disposable tip & holder sets, sample tubes, elution tubes Features 1. High performance of purified DNA in downstream applications such as qpcr. 2. Higher sensitivity. 2. Pre-filled and sealed buffer cartridges prevent contamination. 3. Linear yields from a wide range of viral titers. 4. Walkaway processing enables efficient workflow. Applications The MagCore Viral Kit is designed to allow automated processing of multiple sample types in the same run. Sample types include: 1. Cell-free body fluids 2. Plasma and serum 3. CSF 4. Urine

10 Performance HBV detection by Real-Time PCR The isolations of HBV nucleic acid from samples containing different concentrations of HBV were subsequently detected by Real-Time PCR Sensitivity test for HBV detection Lane 1 1x100 HBV serums. Lane 2 1x101 HBV serums. Lane 3 1x102 HBV serums. Lane 4 1x103 HBV serums. Lane N Negative control. Lane M RBC 100 bp DNA Ladder Marker N M bp Sensitivity test for HCV detection Lane 1 5x100 HCV serums. Lane 6 5x105 HCV serums. Lane 2 5x101 HCV serums. Lane P Positive control. Lane 3 5x102 HCV serums. Lane N Negative control. Lane 4 5x103 HCV serums. Lane M RBC 100 bp DNA Ladder Marker. Lane 5 5x104 HCV serums. M P N bp Figure re1. Nested edpcr CRresults tsof HBV atdiff different en concentrations. Viralnucleicacidswer acids werepurifi purified from samples containing different amount of HBV using MagCore Viral Kit. Fg Figure 2. NestedPCR results of HCV atdifferent concentrations. Viral nucleic acids were purified from samples containing i different amount of fhcv using MagCore Viral Kit. MagCore Specification Cat.No. MVN (36 tests / kit ) / MVN (96 tests / kit ) Cartridge Code : 201 MagCore Viral Kit Running Time: 45 mins for 200μl samples / 56 mins for 400μl samples Cat.No. MVN (36 tests / kit ) / MVN (96 tests / kit ) Cartridge Code : 202 MagCore Viral Kit (Low PCR Inhibition) Running Time: 57 mins for 200μl samples / 66 mins for 400μl samples Cat.No. MVN2400 (96 tests / kit ) Cartridge Code : 210 MagCore Viral Large Volume Kit ( 2.4 ml) Running Time: 90 mins for 2400μl samples Cat.No. MVN1200 (96 tests / kit ) Cartridge Code : 211 MagCore Viral Large Volume Kit (1.2 ml) Running Time: 70 mins for 1200μl samples

11 MagCore For extraction of plant DNA from plant tissues by using MagCore System MagCore Genomic DNA Plant Kit is designed to extract DNA from plant tissues and cells by performing with the MagCore instrument. The provided Filter Column Set can filtrate hard tissue samples to prevent tissue residues from clogging the pipette syringe. The kit contains all required reagents and labware for automated extraction using magnetic-particle technology. Reagents necessary for a complete process are supplied and pre-filled in cartridges, which can be easily loaded into the MagCore instrument. 301 MagCore Genomic DNA Plant Kit For mg fresh tissues or 5-20mg dried plant tissues Contents: pre-filled cartridges, RNase A, GP1 buffer, GP2 buffer, filter column sets, disposable tip & holder sets, sample tubes, elution tubes Features 1. Lysis buffer treats the large amount of polysaccharide samples for an optimal performance. 2. Pre-filled and sealed buffer cartridges prevent contamination. 3. No phenol or chloroform extraction and alchol precipitation. 4. Efficient removal of contaminants and inhibitors. Applications The MagCore Genomic DNA Plant Kit provides purification of DNA from plant tissue, including: 1. Plant cells 2. Plant tissues The high quality DNA obtained by using the MagCore Genomic DNA Plant Kit is suited for many applications, such as: 1. PCR 2. Real-time PCR 3. Genotyping

12 Performance Yields of nucleic acid purified from various sources by MagCore Genomic DNA Plant Kit Sample ID Position Yield (ng/μl) Citrus nobilislour. Leaf 36.5 Carica papaya Leaf 49.2 Lycopersicon esculentum Leaf 71.9 Ctrullus lanatus Leaf 23.4 Anisogonium esculentum Leaf Trachycarpus fortunei Leaf Mentha piperita Leaf 54.6 Anthoceros punctatus Leaf 38.9 Calix babylonica Leaf 80.7 Prnus campanulata Leaf 35.7 Lxeris chinensis Leaf Capsicum annuum Leaf 13.9 Vigna radiata Seed 7.0 Medicago sativa Leaf 21.0 Vigna angularis Seed 5.8 Agaricus bisporus Whole 14.3 MagCore Specification cation Cat.No. MGP-01 (36 tests / kit ) / MGP-02 (96 tests / kit ) Cartridge Code : 301 MagCore Genomic DNA Plant Kit Running Time: 33 mins for 400μl samples

13 MagCore For extraction of total DNA from formalin-fixed paraffin-embedded (FFPE), tissue and forensic samples by using MagCore System MagCore Genomic DNA FFPE One-Step Kit is designed for purification of total DNA from FFPE tissues by using MagCore instruments. It features the method, One-Step Heating, to melt paraffin and lyse tissue samples at the same time without harmful reagents involved such as xylene. Two protocols are designed and optimized for different sizes of tissues: 2 hrs for small samples/ 16 hrs for large samples. DNA will be extracted fast and economically based on the cellulose coated magnetic bead technology. 401 MagCore Genomic DNA Tissue Kit For tissue and forensic samples (up to 40mg) Contents: pre-filled cartridges, filter column sets, GT buffer, proteinase K, PK storage buffer, disposable tip & holder sets, sample tubes, elution tubes New 405 MagCore Genomic DNA FFPE One-Step Kit For FFPE tissue samples Contents: thermostable cap, SULA oil, pre-filled cartridges, filter column sets, GT buffer, proteinase K, PK storage buffer, disposable tip & holder sets, sample tubes, elution tubes Features 1. MagCore Genomic DNA Tissue Kit -It is suitable for various sample types especially including forensic samples. -High quality DNA ready to use for downstream applications. 2. MagCore Genomic DNA FFPE One-Step Kit -A full package for DNA purified from FFPE samples. It starts from the step of neutralizing paraffin to the final DNA extraction. -SULA oil (deparaffinization solution): no xylene involved -Thermostable cap is specially designed to cover the reaction well for an optimal condition where lysis buffer and the FFPE sample can mix properly during the long processing period. -The process to complete is within approximately 3 hours. Application MagCore Genomic DNA Tissue Kit is specific for the following samples: 1. Swab 2. Animal tissue 3. Stool 4. Feed-soil sample 5. Forensic sample

14 Performance 2-hr heating protocol works out There are 2-hour heating and 16-hour heating protocols. It is suggested for a better performance that the 2-hour heating program is for small tissues and the 16-hour program is for large tissues. Genomic DNA with high performance The DNA extracted by MagCore Genomic DNA FFPE One-Step Kit is high yield and suitable for downstream PCR and real-time PCR. A. DNA concentration Whether samples were treated with either a 2-hour or 16-hour heating, yields of DNA extracted by MagCore are mostly higher than the yields of DNA extracted by the competitor. C. Purified DNA ideal for the downstream real-time PCR Whether samples experience a 2-hour or 16-hour heating, the CT values of samples processed by MagCore are mostly lower than the CT values of samples processed by the competitor. C T hour hea ng MagCore samples compe tor Q D. Tissue sample sizes It is shown below that different sizes of tissue samples were processed by a 2-hour or 16-hour heating program. C T hour hea ng samples MagCore 405 compe tor Q DNA (ng/ l) 2-hour hea ng MagCore compe tor Q samples B. The downstream PCR 16-hour hea ng MagCore 405 compe tor Q samples PCR products of DNA purified by MagCore and the competitor brand are confirmed by 1.5% agarose gel electrophoresis. DNA (ng/ l) 2-hour heating Sample Size (cm2) *1 0.8* *1 1.5*2 1.5*2 1.5*2 0.5* *1 16-hour heating Sample Size (cm2) *3 2*2 2*2 2*3 2*2 2*3 1.5*1 0.5* * * *1 MagCore Specification Cat.No. MGT-01 (36 tests / kit ) / MGT-02 (96 tests / kit ) Cartridge Code : 401 MagCore Genomic DNA Tissue Kit Running Time: 33 mins for 400μl samples Cat.No. MGF-01 (36 tests / kit ) / MGF-02 (72 tests / kit ) Cartridge Code : 405 MagCore Genomic DNA FFPE One-Step Kit Running Time: 175 mins for samples with 2-hour heating 16 hrs and 52 mins for samples with 16-hour heating DNA yield gained from 10 mg of mice tissue samples and 0.25 cm tail Sample ID Yield (ng/ul) Liver 17.5 Spleen 17.5 Brain 10 Lung 12.5 Kidney 32.5 Tail 7.5

15 MagCore For extraction of genomic DNA from bacteria by using MagCore System MagCore Genomic DNA Bacterial Kit is designed to extract genomic DNA from Gram positive and Gram negative bacteria. The Kit contains all required reagents and labware for automated extraction using magnetic-particle technology. Reagents necessary for a complete process are supplied and prefilled in cartridges, which can be easily loaded into the MagCore instrument. 502 MagCore Genomic DNA Bacterial kit For cultured bacteria samples (up to 2x10 9 cells) Contents: pre-filled cartridges, lysozyme reaction buffer, proteinase K, PK storage buffer, RNase A, disposable tip & holder sets, sample tubes, elution tubes Features 1. High performance of purified DNA in downstream applications such as qpcr. 2. Pre-filled and sealed buffer cartridges prevent contamination. 3. No phenol or chloroform involved. Applications MagCore Genomic DNA Bacterial Kit is designed to allow automated processing of multiple sample types include: 1. Suspension cultures (up to 2x 10 9 cells) 2. Biological fluids 3. Plate cultures 4. Gram-positive bacteria

16 Performance High performance of purified bacterial DNA in real-time PCR Genomic DNA was purified from Helicobacter pylori sample A and dbi in triplicates t by MagCore Genomic DNA Bacterial Kit. Results of genomic DNA extraction from bacteria samples. Fig. A DNA was purified from different volumes of Bacillus Subtilis suspensions (sample 1 to 5: 025, 0.5, 0.75, 1, 2 ml). Fig. B It is shown that DNA was purified from G(-) E. Coli DH5α (lane 1) and G(+) B. subtilis (lane 2). Fig. C Pareto chart shows DNA recovered with consistent yield and purity. A Well Type Target Ct Interpretati on Label D2 Unknow H.pylori Positive Sample A D3 Unknow H.pylori Positive Sample A D4 Negative H.pylori No Ct Negative Negative Ctrl D5 Unknow H.pylori Positive Sample A B E2 Unknow H.pylori Positive Sample B E3 Unknow H.pylori Positive Sample B E4 Unknow H.pylori Positive Sample B Sample B Sample A C Yields ( g) A260/280 MagCore Specification Cat.No. MGT-01 (36 tests / kit ) / MGT-02 (96 tests / kit ) Cartridge Code : 502 MagCore Genomic DNA Bacterial Kit Running Time: 44 mins for 200μl samples

17 MagCore For total RNA extraction from human whole blood by using MagCore System MagCore Total RNA Whole Blood Kit is specially designed for total RNA purification from up to 0.4ml human whole blood by using MagCore System. RBC's DNase is supplied for your option for the recovery of pure RNA without any DNA contaminants. 601 MagCore Total RNA Whole Blood Kit For whole blood samples (up to 0.4ml) Contents: pre-filled cartridges, RBC lysis buffer, RB buffer, disposable tip & holder sets, sample tubes, elution tubes. Features 1. Reliable processing of up to 0.4 ml human whole blood. 2. No phenol or chloroform involved. 3. Pure RNA: A 260/280 ratio between 1.9 to Ready-to-use RNA in downstream applications. 5. DNA removal treatment. Application Purified RNA ready for use in downstream applications such as: 1. RT-PCR 2. Real-time RT-PCR 3. cdna synthesis

18 Performance Total RNA with high performance Comparison of real-time PCR results of mrna extracted by MagCore and Brand Q mrna was isolated from human whole blood by either the MagCore Total RNA Whole Blood Kit or the Brand Q manual whole blood RNA Kit. Nested RT-PCR analysis RNA eluted in a final volume of 60μl was purified from 400μl whole blood of healthy subjects by the MagCore Total RNA Whole Blood Kit. Eluates of different volumes (10μl, 1μl and 0.1μl) were subjected to reverse transcription. KRAS Sample Ct 1 22 Q1 Q2 N Ct M: 100bp DNA ladder N: negative control P: positive control Performance Total RNA was isolated from human whole blood samples by the employment of MagCore instrument and Total RNA Whole Blood Kit (400 μl sample volume). The yield is 1μg RNA/ 400 μl whole blood. Eight replicates were applied to an agarose gel, showing the purified total RNA without signs of degradation. BAC ( -actin) Sample Ct Q1 Q2 N s 18 s Ct MagCore Specification Cat.No. MGT-01 (36 tests / kit ) / MGT-02 (96 tests / kit ) Cartridge Code : 601 MagCore Total RNA Whole Blood Kit Running Time: 42 mins for 200μl samples without DNase treatment 68 mins for 200μl samples with DNase treatment

19 MagCore For total RNA extraction from cultured cells by using MagCore System MagCore Total RNA Cultured Cells Kit is specially designed for total RNA extraction from up to 1x10 6 cultured cells by using MagCore instrument. RBC's DNase is supplied for your option for the recovery of pure RNA without any DNA contaminants. 610 MagCore Total RNA Cultured Cells Kit For Cultured cell samples (up to 1 x106 cells) Contents: pre-filled cartridges, RB buffer, disposable tip & holder sets, sample tubes, elution tubes Features 1. High quality RNA ready-to-use for downstream applications 2. No phenol or chloroform involved. 3. Pure RNA: A 260/280 ratio between 1.9 to DNA removal treatment. Application Purified RNA ready for use in downstream applications such as: 1. RT-PCR 2. Real-time RT-PCR 3. Microarray target preparation 4. Northern blotting 5. NGS analysis

20 Performance Aanalysis of RNA by Agilent Bioanalyzer 2100 Sample name Sample volume (μl) RNA conc. (ng/μl) RNA quantity (μg) OD260/280 Ratio Bioanalyzer chip lane location 28S/18S Ratio # 1 C1 Q 200 μl # 2 C1 200 μl # 3 T1 Q 200 μl # 4 T1 200 μl # 5 H1 Q 200 μl # 6 H1 200 μl Comparison of RNA by Agilent bioanalyzer Cultured cells of three cell lines (C1, T1 and H1) were used for RNA purification using either the MagCore Total RNA Cultured Cells Kit or Brand Q manual whole blood RNA kit. It is shown that the MagCore Total RNA Cultured Cells Kit can extract RNA of various sizes such as mrna, ribosomal RNA even microrna (mirna) and small interfering RNA (sirna). RIN Bioanalyzer electropherogram C1Q Supplier Q C1 Magcore T1Q T1 H1Q H1 MagCore Specification Cat.No. MGT-01 (36 tests / kit ) / MGT-02 (96 tests / kit ) Cartridge Code : 610 MagCore Total RNA Cultured Cells Kit Running Time: 42 mins for 200μl samples without DNase treatment 73 mins for 200μl samples with DNase treatment