Harvard Medical School 20 Staniford Street Boston, MA 02114

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1 PART I: General Information Name: Office Address: Michael Joseph Young Schepens Eye Research Institute Harvard Medical School 20 Staniford Street Boston, MA Work Fax: Place of Birth: USA Education: 1995 Ph.D., Anatomy, University of Cambridge 1989 B.S., Neuroscience, University of Pittsburgh Post-doctoral training: 1995 Post-doctoral Fellow, Institute of Ophthalmology, University College, London, UK Post-doctoral Associate, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA Academic Appointments: Instructor, Department of Ophthalmology, Harvard Medical School Investigator, Schepens Eye Research Institute Assistant Scientist, Schepens Eye Research Institute Assistant Professor, Department of Ophthalmology, Harvard Medical School Associate Scientist, Schepens Eye Research Institute Associate Professor, Department of Ophthalmology, Harvard Medical School Major Administrative Responsibilities: Member, Immunity, Inflammation, and Transplantation Focus Group, Schepens Eye Research Institute Director, Minda degunzburg Research Center for Retinal Transplantation, Schepens Eye Research Institute Foundation Fighting Blindness, Scientific Advisory Board 2004 NIH Study Section, Ad hoc member, SBIR, Aging and Disease, Special Emphasis Panel/Scientific Review Group 2004 ZRG The Glaucoma Foundation, Scientific Advisory Board Member, Neural & Vascular Biology Focus Group, Schepens Eye Research Institute

2 2005- Member, Retinal Laser Injury Focus Group, Schepens Eye Research Institute 2005 NIH Study Section, Ad hoc member, SBIR, Aging and Disease, Special Emphasis Panel/Scientific Review Group 2005/05 ZRG1 BDA-B 2005 NIH Study Section, Ad hoc member, Stem Cells and Development, Special Emphasis Panel/Scientific Review Group 2005/05 NCF (S) 2005 Johnson & Johnson Internal Ventures, Stem Cell Program, Scientific Advisory Board Major Committee Assignments: Schepens Eye Research Institute Internal Communications Committee, Member 1997-present Responsible Conduct in Research Committee, Member Animal Care and Use Committee, Member CORE Grant Committee, Member 2000-present Seminar Committee, Chair Research Planning and Review Committee, Member 2005-present Training Committee, Member Professional Society Involvement: Society for Neuroscience, Member Association for Research in Vision & Ophthalmology, Member Association of Immunologists, Member International Society for Stem Cell Research, Member International Society for Ocular Cell Biology, Member Community Service Related to Professional Work: 2000-present Frequent Speaker for Lions Eye Research Foundation Speaker, SERI Florida Outreach Mission 2005 The Longwood Seminar Series, Harvard Stem Cell Institute, Stem Cells: Hope Beyond the Hype 2008 Speaker, SERI Florida Outreach Mission 2009 Speaker, Foundation Fighting Blindness Editorial Boards: Ad hoc reviewer, Nature, Journal of Neuroscience, Vision Research, Investigative Ophthalmology and Vision Science, Experimental Eye Research, Brain Research, STEM CELLS, Nature Biotechnology, Experimental Neurology Editorial Board, Journal of Ocular Biology, Diseases and Informatics Editorial Board, Investigative Ophthalmology and Vision Science Editorial Board, Progress in Retinal and Eye Research

3 Award and Honors: 1986 Departmental Award, Biological Sciences, University of Pittsburgh 1989 Dean's Fellowship, School of Medicine, University of Pittsburgh 1992 Chibnall Travel Fellowship, Clare College, University of Cambridge 1995 National Science Foundation/NATO ASI Travel Fellowship 2001 Leslie W. Nesmith Lectureship (Schepens International Society) 2003 New York Eye & Ear Infirmary, Visiting Professor of Ophthalmology 2005 HMS Leadership Development for Physicians and Scientist 2008 Research to Prevent Blindness, Special Research Scholar Award PART II: Research, Teaching, and Clinical Contributions A. Narrative Report of Research My laboratory studies repair of the mature central nervous system, specifically following degeneration that occurs in the retina during disease or injury. We have focused on the use of stem or progenitor cells, which we have isolated from a number of regions of the neuraxis of several different mammalian species. During the last 5 years, work in my lab has established that neural stem or progenitor cells overcome the barrier to morphological integration present in the mature mammalian retina. We have also demonstrated that neural stem cells are an inherently immune privileged tissue, and survive in conventional sites in allogeneic recipients. We have isolated stem cell from the mouse, pig, and human retina, and shown that such cells are capable of photoreceptor differentiation. We have now embarked upon a series of studies in the pig retina, with the goal of establishing functional connectivity between donor retinal stem cells and the mature, diseased host retina. By using large animals such as the domestic pig, we can take advantage of 1) the availability transgenic donor and hosts, so that we can graft GFP + donor cells into RP porcine recipients; 2) the large eye size that allows traditional pars plana vitrectomy and other retinal surgical techniques; and 3) the ability to evaluate the functional impact of the graft with techniques such as multifocal ERG. This approach will allow us to make important steps toward our goal of functional restoration of vision. B. Funding Information (Research) Current: Continuous Five Year Renewal Schepens Eye Research Institute, Principal Investigator, Endowed Directorship, degunzburg Research Center for Retinal Transplantation 07/01/03-06/30/08 Department of Defense, Co-Investigator, Laser Injury and CNS Stem Cells 03/01/02-06/30/09 Richard and Gail Siegal Foundation, Principal Investigator, CNS Stem Cells, Tissue Engineering and Glaucoma

4 06/01/06-06/30/09 Foundation Fighting Blindness, Principal Investigator Stem Cells in Retinal Degeneration 1/01/07-12/31/11 Lincy Foundation/ Discovery Eye Foundation, Principal Investigator, Tissue Engineering for Retinal Degeneration in a Pig Model 06/01/08-05/31/09 Research to Prevent Blindness Special Scholar Award Report of Current Research Activities: -Isolation and characterization of retinal stem cells from mice, pigs, and humans, Principal Investigator -Generation of retinal neurons from embryonic stem cells, Principal Investigator -Transplantation of rodent and pig retinal stem cells to disease models, Principal Investigator -Bioengineering of composite grafts, Co-Principal Investigator -Slow release polymer delivery of bioactive compounds in glaucoma models, Principal Investigator -Matrix metalloproteinases in neuronal plasticity, Principal Investigator D. Report of teaching: 1. Local contributions 2002 Biology of Ophthalmic Disease, Department of Ophthalmology, HMS. Stem cells and retinal implants (with Prof J. Rizzo) 2001 Visiting Professor of Ophthalmology, New York Eye and Ear Infirmary 2004 Biology of Ophthalmic Disease, Department of Ophthalmology, Harvard Medical School 2005 The Longwood Seminar Series, Harvard Stem Cell Institute, Stem Cells: Hope Beyond the Hype 2005 Molecular Bases of Eye Disease, Department of Ophthalmology, HMS Stem cells and retinal repair (with Dr. I. Kim) 2006 Molecular Bases of Eye Disease, Department of Ophthalmology, HMS Retinal Degeneration (with Prof. E. Berson) 2007 Molecular Bases of Eye Disease, Department of Ophthalmology, HMS Retinal Degeneration (with Prof. E. Berson) 2008 Molecular Bases of Eye Disease, Department of

5 Ophthalmology, HMS Ocular Repair (with James Zeiske) Advisees and Trainees: 1997 Serena Shi; Undergraduate, MIT Dept of Brain and Cognitive Sciences 1997 Semi Lee; Undergraduate, MIT Dept of Brain and Cognitive Sciences Simon Whiteley. Postdoctoral fellow, presently with Informed Direct Hiroyuki Mizumoto. Postdoctoral fellow, presently a faculty member, Department of Ophthalmology, Asahiakwa, Japan Keiko Mizumoto. Postdoctoral fellow, presently a faculty member, Department of Ophthalmology, Asahiakwa, Japan Yasuo Kurimoto, Postdoctoral fellow, presently Director of Ophthalmology, Department of Ophthalmology, Kobe City General Hospital, Japan Derek Lauritzen, Postdoctoral fellow, presently a faculty member at Rocky Mountain Eye Institute Tasneem Zahir, Postdoctoral fellow, presently a postdoctoral fellow at the Univ. of Toronto Tat Fong Ng, Investigator, presently in biotech Ali Khoobehi,, Postdoctoral fellow, presently a resident in surgery, NYU School of Medicine Minoru Tomita, Postdoctoral fellow, presently faculty member, University of Osaka Matt Ward, Undergraduate, presently medical student, University of Utah Joanne Yau, Medical student, presently medical student, University of Melbourne, Australia Yiqin Zhang, Postdoctoral fellow, presently Scientist, Novartis Nadia Waheed, Postdoctoral fellow, presently Ass. Prof., Cole Eye Institute Lucia Sobrin, Postdoctoral fellow, presently Instructor, MEEI Conan Young, Visiting Scientist, presently Scientist, Biotech Michael Moore, Postdoctoral Fellow (co-advisee with R. Langer, MIT), Ass. Prof., Tulane Caihui Jiang, Postdoctoral Fellow, Associate Professor, China Xinmei Zhang, Postdoctoral Fellow Ji Yeon Kim, Postdoctoral Fellow Unber Aftab, Postdoctoral Fellow, in residency Budd Tucker, Postdoctoral Fellow Stephen Redenti, Postdoctoral Fellow Sharon Bakalash, Postdoctoral Fellow Markus Tackenburg, Predoctoral Fellow, in medical school Scott Schmidt, Predoctoral Fellow, in medical school Jesse Swift, Predoctoral Fellow. In medical school

6 William Neeley, Postdoctoral Fellow (co-advisee with R. Langer, MIT), in law school 2. Local, Regional, national, and international contributions Invited Presentations and Seminars: Local 1999 Invited Lecture, Human Embryonic Stem Cells Symposium, SERI 1999 Invited Lecture, Harvard Medical School Press Conference 1999 Seminar, HMS Department of Ophthalmology Retreat 2008 Seminar, HMS Department of Ophthalmology Retreat Regional 2000 Seminar, Biogen (Cambridge) 2001 Invited Lecture, New England Eye Center 2004 Invited Lecture, Massachusetts Institute of Technology, Ch. Eng. National 1999 Invited Lecture, ARVO, Stem Cell Symposium 1999 Seminar, Iowa State University 1999 Invited Lecture, Research to Prevent Blindness Press Seminar 2000 Invited Lecture, Foundation Fighting Blindness/Stem Cell Conference 2001 Invited Lecture, ARVO Stem Cell Biology Course 2001 Invited Lecture, Schepens International Society 2001 Invited Lecture, New England Eye Center 2001 Invited Lecture, LSU Eye Center 2002 Organizer, ARVO meeting on Retinal Rescue, Stem Cell Transplants 2002 Invited Lecture, Bascom Palmer Eye Hospital, University of Miami 2002 Invited Lecture, Foundation Fighting Blindness, Salt Lake City 2002 Invited Lecture, The Glaucoma Foundation Workshop, Chicago, Il 2003 Invited Lecture, University of Missouri, Columbia 2003 Invited Lecture, UCLA Medical Center, Harbor Beach 2003 Invited Lecture, Western Eye Conference, Laguna Beach 2003 Invited Lecture/Co-Organizer, Glaucoma Fnd. Think Tank, Boston 2003 Invited Lecture/Panelist, WAG Conference on Emerging Therapies 2003 Visiting Professor of Ophthalmology, New York Eye and Ear Infirmary 2003 Invited Lecture, McGowan Center for Regenerative Medicine, Pittsburgh 2004 Invited Lecture, The Wilmer Eye Hospital, Johns Hopkins Medical School 2004 Invited Lecture, Unite for Sight conference, NYU 2004 Invited Lecture/Co-Organizer, Glaucoma Fnd. Think Tank, NY, NY 2004 Invited Lecture, UCLA Medical Center, Harbor Beach 2004 Invited Lecture, Cole Eye Institute, Cleveland Clinic 2005 Invited Lecture, Unite for Sight conference, Harvard 2005 Invited Lecture, Swine in Biomedical Research, Chicago 2005 Invited Lecture, American Ophthalmology Society, Sea Island, GA 2005 Invited Lecture, Case Western Reserve University 2005 Invited Lecture, Cole Eye Institute, Cleveland Clinic 2005 Invited Lecture, Vanderbilt University 2005 Invited Lecture, University of Pennsylvania 2006 Invited Lecture, University of Utah 2006 Invited Lecture, University of Louisville 2007 Organizer, Western Eye Conference 2007 Invited Participant, National Institute of Aging workshop, Cell Fate Determination in the Elderly

7 2008 Invited Lecture, Columbia University, Dische Minisymposium 2008 Invited Lecture, Washington Univ., St Louis 2008 Invited Lecture, ARVO Course, Eye Cancer 2009 Invited Lecture, Beckman Initiative for Macular Research, UC Irvine 2009 Invited Lecture, UC Santa Barbara International 2000 Invited Lecture, International Congress on Eye Research 2000 Seminar, Institute of Ophthalmology, London 2000 Seminar, University of Zurich, Brain Research Institute 2001 Seminar, University of Copenhagen Eye Pathology Institute 2001 Seminar, University of Lund Department of Ophthalmology 2002 Organizer, ICER, Stem Cell Biology session 2002 Invited Lecture, German Vitreo-Retinal Society, Marburg, Germany 2002 Invited Lecture, Foundation Fighting Blindness, Lund, Sweden 2003 Invited Lecture, Workshop on Cell Transplantation, Koln 2003 Invited Lecture, University of Amsterdam Eye Institute 2003 Invited Lecture, Euro. Ped. Ophthalmol. Society, Regensburg, Germany 2004 Organizer, ICER, Stem Cell Biology session 2004 Invited Lecture, Vision Down Under conference, Fraser I, Australia 2004 Invited Lecture, Swedish Consulate, Santa Monica, CA, Stem cells 2005 Invited Lecture, Novartis Ophthalmics Lecture, ASRP, Montreal 2005 Invited Lectures, FFB Ireland, Dublin 2006 Invited Lecture, JDRF Conference, University of Oxford 2006 Invited Lecture, ISOCB Conference, University of Cambridge 2006 Invited Lecture, German Ophthalmological Society 2006 Invited Lecture, University of Paris (France) 2007 Organizer, Singapore Asia/Arvo Conference 2008 Invited Lecture, Nippon Medical School, Tokyo 2008 Invited Lectures, World Congress of Ophthalmol, Hong Kong 2008 Invited Lecture, Completense University, Madrid 2009 Invited Lectures, XXXII SIMASP, Sao Paulo, Brazil 2009 Invited Lecture, German Ophthalmological Society, Leipzig 2009 Invited Lecture, Ocular Prosthetics PASI, Argentina 2009 Invited Lecture, Univ. of Dresden, Germany 2009 Invited Lecture, Bali Asia/Pacific Ophthalmology Conference PART III: Bibliography Peer-Reviewed Publications in print or other media 1. Young, M. J., K. Rao and R. D. Lund Integrity of the blood-brain barrier in retinal xenografts is correlated with the immunological status of the host. J. Comp. Neurol. 283: Young, M. J. and R. D. Lund The anatomical substrates subserving the pupillary light reflex in rats: origin of the consensual response. Neurosci. 62: Chan, K. M. C., M. J. Young and R. D. Lund Interactive events subserving the pupillary light reflex in pigmented and albino rats. Euro. J. Neurosci. 7: Sasaki, H., P.J. Coffey, M. P. Villegas-Perez, M. Vidal-Sanz., M.J. Young, R. D. Lund, and Y. Fukuda Light induced EE desynchronization and behavioural arousal in rats with restored retinocollicular projection by peripheral nerve graft. Neurosci. Letters 218: Young, M. J. and R. D. Lund Parameters of transplant-mediated

8 pupilloconstriction in rats with unilateral olivary pretectal lesions. J Comp. Neurol. 388: Young, M. J. and R. D. Lund The retinal ganglion cells that drive the pupilloconstrictor response in rats. Brain Res. 787: Whiteley, S. J. O., M. J. Young, T. M. Litchfield and R. D. Lund Changes in the pupillary light reflex of pigmented Royal College of Surgeons rats with age. Exp. Eye Res. 66: Young, M.J., R.K.K. Lee, S. Jhaveri and R.J. Wurtman Intracellular and cell-surface distribution of amyloid precursor protein in cortical astrocytes. Brain Res. Bull. 50: Wenkel, H., J.W. Streilein, and M.J. Young Systemic immune deviation in the brain that does not depend on the integrity of the blood brain barrier. J. Immunol. 164: Young, M.J., J. Ray, S.J.O. Whiteley, H.J. Klassen, F.H. Gage Integration of transplanted neural progenitor cells into the retina of immature and mature dystrophic rats. Molecular and Cellular Neuroscience 16: Shatos, M. A., K. Mizumoto, H. Mizumoto, Y. Kurimoto, H. Klassen and M. J. Young Multipotent stem cells from the brain and retina of green mice e-biomed: The Journal of Regenerative Medicine. vol. 2: Mizumoto, H., K. Mizumoto, S. J. O. Whiteley, M. Shatos, H.J. Klassen, and M.J. Young Transplantation of human neural progenitor cells to the vitreous cavity of the Royal College of Surgeons rat. Cell Transplantation 10: Klassen, H., S.J.O. Whiteley, M.J. Young, and R.D. Lund Graft location affects functional rescue following RPE cell transplantation in the RCS rat. Experimental Neurology 169(1): Whiteley, S. J. O., H.J. Klassen, P.W Coffey, and M.J. Young Photoreceptor rescue after low-dose intravitreal Il-1ß injection in the RCS rat. Exp. Eye. Res. 73: Anosova, N., Illegens, B-MW, Boisérault, F., Fedoseyeva, E.V., Young, M.J., and G. Benichou. (2001) Antigenicity and immunogenicity of allogeneic retinal transplants. Journal of Clinical Investigation 108: Klassen, H., M. R. Schwartz, A. Bailey, and M. J. Young. (2001) Surface markers expressed by multipotent human and mouse neural progenitor cells include tetraspanins and non-protein epitopes. Neuroscience Letters 312(3): Lu, B., Kwan, T., Kurimoto, Y., Shatos, M., Lund, R.D., and M.J. Young. (2002) Transplantation of neural stem cells from GFP transgenic mice into the eyes of rd mice. Brain Research 943: Ng TF, Osawa H, Hori J, Young M.J., Streilein JW. (2002) Allogeneic neonatal neuronal retina grafts display partial immune privilege in the subcapsular space of the kidney. J. Immunol. 169(10): Van Hoffelen SJ, Young M.J., Shatos MA, Sakaguchi DS. (2003) Incorporation of murine brain progenitor cells into the developing mammalian retina. Invest. Ophthalmol. Vis. Sci. 44(1): Mizumoto, H., K. Mizumoto, M. Shatos, H.J. Klassen, and M.J. Young. (2003) Retinal transplantation of neural progenitor cells derived from the brain of GFP transgenic mice Vision Research. 43: Hori, J, T. F. Ng, M. Shatos, J. W. Streilein, and M. J. Young. (2003) Neural stem cells lack immunogenicity and resist destruction as allografts. Stem Cells. 21: Klassen, H., K. L. Imfeld, J. Ray, M. J. Young, M. A. Berman, and F. H. Gage. (2003) Adult hippocampal progenitor cells are non-immunogenic in mixed co-cultures Vision Research. 43:

9 23. Klassen, H., Imfeld, K., Kirov, I., Gage, F., Young, M.J., Berman, M. (2003) Expression of cytokines by multipotent human neural progenitor cells. Cytokine. 22: Klassen, H., Ng, T.F., Kurimoto, Y., Kirov, I., Shatos, M., Coffey, P. and Young, M. J. (2004) Multipotent retinal progenitors express developmental markers, differentiate into retinal neurons, and preserve light-mediated behavior." Invest Ophthalmol Vis Sci. 45 (11): Sakaguchi, D. S., S. J. Van Hoffelen, E. Theusch, E. Parker, J. Orasky, M. M. Harper, A. Benediktsson, M. J. Young. (2004) Transplantation of neural progenitor cells into the developing retina of the Brazilian opossum: an in vivo system for studying stem/progenitor cell plasticity. Dev Neurosci 26(5-6), Lavik, E. B.,H. Klassen, K. Warfvinge, R. Langer, and M. J. Young. (2005) Fabrication of Degradable Polymer Scaffolds to Direct the Integration and Differentiation of Retinal Progenitors. Biomaterials, 26 (16): Zahir, T., Klassen, H. & Young, M.J. (2005) Effects of ciliary neurotrophic factor on differentiation of late retinal progenitor cells. Stem Cells 23, Warfvinge K, Kiilgaard JF, Lavik E, Scherfig E, Langer R, Klassen H, Young M.J. (2005) Retinal progenitor cell xenografts to the pig retina: morphological integration and cytochemical differentiation. Arch. Ophthalmol, 123: Schwartz, P, Nethercott, H., Kirov, I., Ziaeian, B., Young, M.J. and H. Klassen Expression of neurodevelopmental markers by cultured porcine neural precursor cells. Stem Cells, 23: Tomita, M., Lavik, E., Zahir, T., Klassen, Langer, R., and Young, M.J Retinal progenitor cell transplantation using biodegradable polymers. Stem Cells 23: Burdick, J.A., M. Ward, E. Liang, M.J. Young, R. Langer Stimulation of Neurite Outgrowth by Neurotrophins. Delivered from Degradable Hydrogels. Biomaterials, 27: Ford, MC, JP Bertram, S Hynes, M Michaud, Q Li, M.J. Young, SS Segal, JA Madri & EB Lavik. A macroporous hydrogel for the co-culture of neural progenitor and endothelial cells to form functional vascular networks in vivo. Proc. Nat. Acad. Sci ;103(8) Tomita, M., T. Mori, K. Maruyama, T. Zahir, M. Ward, A. Umezawa, M. J. Young A comparison of neural differentiation and retinal transplantation with bone marrow-derived cells and retinal progenitor cells. Stem Cells, 24(10): Warfvinge, K., J. F. Kiilgaard, H. Klassen, P. Zamiri, E. Scherfig, J. W. Streilein, J. U. Prause, M. J. Young Retinal progenitor cell xenografts to the pig retina: immunological reactions. Cell Transplantation, 15 (7) Zahir, T., M. Tomita, H. Klassen, M. J. Young Sorbitol promotes differentiation of late retinal progenitor cells into Müller glia in vitro. Molecular Vision. 12: Ward, M. S., Khoobehi, A., Lavik, E. B., Langer, R. and Young, M. J Neuroprotection of retinal ganglion cells in DBA/2J mice with GDNFloaded biodegradable microspheres. J Pharm Sci. 96(3): Klassen, H.K, J. F. Kiilgaard, T. Zahir, B. Ziaeian, I. Kirov, E. Scherfig, K. Warfvinge and

10 M. J. Young Progenitor cells from the porcine neural retina express photoreceptor markers after transplantation to the subretinal space of allorecipients. Stem Cells. 25: Zhang, Y, B Tucker, HJ Klassen, MT Perez, and MJ Young CNS progenitor cells promote a permissive environment for neurite outgrowth via an MMP-2 dependent mechanism. J. Neuroscience. 27: Tao, S., C. Young, S. Redenti, Y. Zhang, H. Klassen, T. Desai and MJ Young Survival, migration and differentiation of retinal progenitor cells transplanted on micro-machined poly(methyl methacrylate)scaffolds to the subretinal space. Lab on a Chip. 7: Klassen, H., Schwartz, P, Nethercott, H., Ziaeian, B., Young, M.J, Narfstrom, K Neural precursors isolated from the developing cat brain show retinal integration following transplantation to the retina of the dystrophic cat. Veterinary Ophthalmology. 10: Ng, TF, E Lavik, H Keino, AW Taylor, R Langer, and MJ Young Creating an immune privileged site using retinal progenitor cells and biodegradable polymers. Stem Cells 25: Jiang, C., Moore, M. J., Zhang, X., Klassen, H., Langer, R. and Young, M Intravitreal injections of GDNF-loaded biodegradable microspheres are neuroprotective in a rat model of glaucoma. Mol Vis 13: Neeley, W. L., Redenti, S., Klassen, H., Tao, S., Desai, T., Young, M. J. and Langer, R A microfabricated scaffold for retinal progenitor cell grafting. Biomaterials 29: Dunn-Thomas, T., D. Dobbs, D. Sakaguchi, M.J.Young, V. Honavar, M. H. West Greenlee Proteomic characterization of retinal progenitor cells compared to brain progenitor cells. Stem Cells and Development. 17: Tucker, B., H. Klassen, L. Yang, D.F. Chen, M.J. Young Elevated MMP expression in the MRL mouse retina creates a permissive environment for photoreceptor transplantation. Invest. Ophthalmol. Vis. Sci. 49: Redenti, S, S Tao, J Yang, P Gu, H Klassen, S Saigal, T Desai and M J Young Retinal tissue engineering using mouse retinal progenitor cells and a novel biodegradable, thin-film poly(e-caprolactone) nanowire scaffold. J Ocular Biol Dis Inf. 1: Klassen, H, K Warfvinge, PH Schwartz, JF Kiilgaard, N Shamie, C Jiang, E Scherfig, RS Prather, and MJ Young Isolation of progenitor cells from GFP-transgenic pigs and transplantation to the retina of allorecipients. Cloning and Stem Cells. 10 (3): Ronen, A, S Bakalash, MJ Young, M Schwarz A restrictive role for tolllike receptor 4 (TLR4) in retinal progenitor cell proliferation. J. Cell Biology. 183(3): p Saint-Geniez, M., A.S. Maharaj, T.E. Walshe, B.A. Tucker, E. Sekiyama, T. Kurihara, D.C. Darland, M.J. Young, and P.A. D'Amore, Endogenous VEGF is required for visual function: evidence for a survival role on muller cells and photoreceptors. PLoS ONE, (11): p. e Redenti, S., W.L. Neeley, S. Rompani, S. Saigal, J. Yang, H. Klassen, R. Langer, and

11 M.J.Young, Engineering retinal progenitor cell and scrollable poly(glycerolsebacate) composites for expansion and subretinal transplantation. Biomaterials, (20): p Kyhn, M.V., K. Warfvinge, E. Scherfig, J.F. Kiilgaard, J.U. Prause, H. Klassen, M. Young, and M. la Cour, Acute retinal ischemia caused by controlled low ocular perfusion pressure in a porcine model. Electrophysiological and histological characterisation. Exp Eye Res, (6): p Tucker, B.A., S.M. Redenti, C. Jiang, J.S. Swift, H.J. Klassen, M.E. Smith, G.E. Wnek, and M.J. Young, The use of progenitor cell/biodegradable MMP2-PLGA polymer constructs to enhance cellular integration and retinal repopulation. Biomaterials, (1) p Tackenberg, M.A., B.A. Tucker, J.S. Swift, C. Jiang, S. Redenti, K.P. Greenberg, J.G. Flannery, A. Reichenbach, and M.J. Young, Muller cell activation, proliferation and migration following laser injury. Mol Vis, : p Schmitt, S.G., U. Aftab, C. Jiang, S. Redenti, H. Klassen, E. Miljan, J. Sinden, and M.J. Young, Molecular characterization of human retinal progenitor cells. Invest Ophthalmol Vis Sci, Redenti, S., W.L. Neeley, S. Rompani, S. Saigal, J. Yang, H. Klassen, R. Langer, and M.J. Young, Engineering retinal progenitor cell and scrollable poly(glycerol-sebacate) composites for expansion and subretinal transplantation. Biomaterials, (20): p Aftab, U., C. Jiang, B. Tucker, J.Y. Kim, H. Klassen, E. Miljan, J. Sinden, and M. Young, Growth kinetics and transplantation of human retinal progenitor cells. Exp Eye Res, (3): p Kyhn, M.V., H. Klassen, U.E. Johansson, K. Warfvinge, E. Lavik, J.F. Kiilgaard, J.U. Prause, E.Scherfig, M. Young, and M. la Cour, Delayed administration of glial cell line-derived neurotrophic factor (GDNF) protects retinal ganglion cells in a pig model of acute retinal ischemia. Exp Eye Res, Gu, P., J. Yang, J. Wang, M.J. Young, and H. Klassen, Sequential changes in the gene expression profile of murine retinal progenitor cells during the induction of differentiation. Mol Vis, : p Non-peer reviewed scientific or medical publications/materials in print or other media 1. Lund, R. D., H. J. Klassen and M. J. Young Retinal transplantation and the pupillary light reflex. The Autonomic Nervous System, v13: "The Innervation of the Eye" G. Birnstock and A. Sillito, eds. 2. Caballero, S., Sengupta, N., Crafoord, S., Lund, R., Kruse, F. E., Young, M.J., Grant, M. B The many possible roles of stem cells in age-related macular degeneration. Graefes Arch Clin Exp Ophthalmol 242(1), Klassen, H., Sakaguchi, D., Young, M.J Stem cells and retinal repair. Progress in Retinal and Eye Research. 23: Sakaguchi, D.S., S.J Van Hoffelen., S.D. Grozdanic, Y.H. Kwon, R.H. Kardon, and M.J. Young, Neural progenitor cell transplants into the developing and

12 mature central nervous system. Ann N Y Acad Sci, : p Young, M.J., Borrás, T., Walter, M., and Ritch, R. Tissue Bioengineering: Potential Applications to Glaucoma, Arch. Ophthalmol, in press. 6. Klassen, H., Young, M.J., Ritch, R., Richards, J., Borras, T., Levin, L. Retinal stem cell research. In Vitreoretinal Surgical Techniques, 2nd edition, Peyman, Meffert, and Conway, eds., in press. 7. Yau, J., Klassen, H., Zahir, T., and Young, M.J Stem cells and retinal transplantation. In Retinal Degenerations: Biology, Diagnostics, and Therapeutics, Tombran-Tink, J. and Barnstable, C., pp Young, M.J. Stem cells in the mammalian eye: a new tool for retinal repair Acta Pathologica, Microbiologica et Immunologica Scandinavica. 113: Young, M. J., H. J. Klassen Polymeric delivery of neuroprotective compounds to the vitreous cavity: a potential treatment for glaucoma. Arch Soc Esp Oftalmol 81(8), Ng, T.F., Klassen, H.,Hori, J., and M.J. Young. Retinal Transplantation. (2007) in Immune Response and the Eye, 2nd Edition. Edited by JY Niederkorn (Dallas TX) and H Kaplan (Louisville, KY). A volume in the series Chemical Immunology and Allergy. Karger AG (Basel) 92: Hori, J., Ng, T. F., Shatos, M., Klassen, H., Streilein, J. W. and Young, M. J. (2007). Neural progenitor cells lack immunogenicity and resist destruction as allografts Ocul Immunol Inflamm 15: