STATE OF THE ART ON BIOBANKING INFRASTRUCTURE DEVELOPMENT AT IZSLER: BIOLOGICAL SPECIMEN COLLECTION, QUALITY CONTROL AND MANAGEMENT
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2 ESBB Annual Meeting - Marseille, November th, 2011 STATE OF THE ART ON BIOBANKING INFRASTRUCTURE DEVELOPMENT AT IZSLER: BIOLOGICAL SPECIMEN COLLECTION, QUALITY CONTROL AND MANAGEMENT Maura Ferrari Istituto Zooprofilattico della Lombardia e dell Emilia Romagna Brescia (IZSLER), Italy
3 IZSLER Istituto Zooprofilattico Sperimentale della Lombardia e dell Emilia Romagna Veterinary Government Organization supported by Ministry of Health Guarantees Veterinary Service activities Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
4 Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
5 Where is IZSLER? LOMBARDIA: Brescia, Headquarter 8 Offsite Laboratories EMILIA ROMAGNA: 8 Offsite Laboratories Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
6 IZSLER Activities Animal health Food safety Zootechnic hygiene Animal welfare Several Reference Centres: Italian Ministry of Health, OIE, FAO Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
7 Livestock Population Lombardia Emilia Romagna % of italian population Bovine 1,526, , Equine 43,257 28, Swine 5,040,076 1,275, Ovine 211,825 86, Rabbit 611, , Avian Species 49,296,886 34,513, Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
8 Background Several laboratories involved in different branches Wide availability of biological resources and samples Development of a Biobank Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
9 Outline : Steps 1. Information processing / Records / Documentation 2. List of all the biological samples and resources at IZSLER 3. Selection of resources, materials to be collected, barcoding 4. Development of quality control criteria 5. Development of biobank infrastructure and backup system 6. Quality assurance system (ISO ), standard operating procedures (SOPs) Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
10 Step 1. BioBank Architecture The Architecture is an open system The system can communicate with other systems by applicative cooperation Legacy Image External system (monitoring, other biorepositories,.. ) Multichannel (desktop, internet, mobile,..) Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
11 Biobanking Architecture Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
12 Step 2. Census Algae: 294 Bacteria: 29,031 Chemical substrates: 26 Chlamydia: 18 Cell cultures: 602 Conjugated Ig: 40 Experimental immune sera: 380 Field sera: 382 Fungi: 25 Genomic sequences: 239 Monoclonal antibody: 64 Hybridomas: 6,427 Laboratory animals: 11 Mycoplasmas: 177 Metazoan parasites: 113 Nucleic acids: 24 Protozoa parasites: 63 Recombinant plasmids: 153 Prions: 58 Organs and tissues: 264 Viral pathological specimens: 426 Viruses: 5,271 Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
13 42,683 types of different samples Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
14 Step 3. Selection of the resources / specimens Preparation of forms specific for each biological sample Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
15 Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
16 Step 4. Development of quality control criteria and labelling Working groups for specimen preparation and quality control criteria: characterization typing absence of extraneous agents (viruses, mycoplasma, bacteria) Barcoding labelling Time consuming work with high labour costs Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
17 Barcode video Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
18 Currently Algae: 16 Bacteria: 196,000 Chemical substrates: 26 Chlamydia: 18 Cell cultures: 604 Conjugated Ig: 40 Experimental immune sera: 380 Field sera: 382 Fungi: 25 Genomic sequences: 239 Monoclonal antibody: 64 Hybridomas: 6,427 Laboratory animals: 11 Mycoplasmas: 177 Metazoan parasites: 113 Nucleic acids: 24 Protozoa parasites: 63 Recombinant plasmids: 153 Prions: 58 Organs and tissues: 264 Viral pathological specimens: 3 Viruses: 38 Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
19 Step 5. Identification of Biobank Infrastructures storage rooms + 4 C - 20 C Liquid Nitrogen Room temperature - 80 C Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
20 Step 6. Quality assurance system SOP preparation Biobank certification ISO Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
21 Field samples Reception Laboratory automation sample distribution and labelling Research Biobanking Sample processing: diagnostic services Datebase with traceability of all steps Veterinarians Clinical result transmission Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
22 Objective Construction of a new Italian Animal BioBanking Infrastructure Development of an European Animal BioBanking Infrastructure (ESFRI, BBMRI) Integration of international resources Improvement of scientific cooperation Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
23 Perspectives: Data and Samples Researcher Department Organization National International Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
24 Users Advantages Researchers Clinics involved in diagnostic laboratories Commercial companies Availability of biological resources, with well defined characteristics Biolological resource centres: reference material over time Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
25 Conclusions Bio-specimen resources Basic tool for Scientific Community and priceless Translational studies New perspectives in the future Improvement of Public Health Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
26 Thanks Researchers (our lab) Tina Lombardo Massimo Amadori Riccardo Villa Silvia Dotti IT Team Riccardo Possenti Giorgio Bontempi Stefano Vigliani Ricardo Kligman Roberta Pavesi All colleagues of IZSLER Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
27 Maura Ferrari. IZSLER biobanking ESBB Annual Meeting - Marseille, November 16-19th,
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