OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : :31:01 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Contagious bovine pleuropneumonia Address of laboratory: Via Campo Boario Teramo ITALY Tel.: Fax: address: m.scacchia@izs.it Website: Name (including Title) of Head of Laboratory (Responsible Official): Prof Mauro Mattioli, DVM, General Director Name (including Title and Position) of OIE Reference Expert: Massimo Scacchia, DVM, Head of Animal Health Laboratory. Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/No) Total number of test performed last year Indirect diagnostic tests Nationally Internationally CFT c-elisa 0 79 Immuno-blotting 0 19 Direct diagnostic tests Nationally Internationally PCR 2 20 Mmm isolation 2 17 RT-PCR 2 67 PCR-RFLP (identification) 0 67 Immunohistochemistry Microarray 0 3 NGS 0 29 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? NOTE: Currently, there are 22 laboratories that produce Standard Reference Reagents officially recognised by the OIE for 19 2 OIE Reference Laboratory Reports Activities, 2016

3 diseases/pathogens. Please click the following link to the list of OIE-approved International Standard Sera: If the reagent is not listed on this page, it is NOT considered OIE-approved. The next two questions allow you to indicate non-oie-approved diagnostic reagents. Disease Test Available from Contagious bovine pleuropneumonia Dr Massimo Scacchia CESME, Istituto Zooprofilattico Both irradiated and non-irradiated sera are available. Sperimentale dell Abruzzo e del Please contact the Reference Laboratory (Dr Pini) for Molise 'G. Caporale', Via Campo advice on their suitability for different types of Boario, serological test (complement fixation test, indirect or Teramo, Italy competitive enzyme-linked immunosorbent assay) Tel: ( ) Fax: ( ) m.scacchia@izs.it Type of reagent available Related diagnostic test Produced/ Supply imported Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) Name of recipient OIE Member Countries Positive serum CFT Produced TURKEY Positive serum CFT Produced SENEGAL Negative serum CFT Produced TURKEY Negative serum CFT Produced SENEGAL 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? OIE Reference Laboratory Reports Activities,

4 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) No. of recipient OIE Member Countries Region of recipients Mmm Antigen FdC Produced 5 ml 20 ml 2 Africa Americas Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? No 5. Did your laboratory supply vaccines to OIE Member Countries? No ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? No 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? No ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 4 OIE Reference Laboratory Reports Activities, 2016

5 Name of OIE Member Country seeking assistance Date (month) No. samples received for provision of diagnostic support No. samples received for provision of confirmatory diagnoses NIGERIA October 202 sera, 17 strains ANGOLA July 300 sera, 2 strains SENEGAL April 39 strains December 4 strains 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided CBPP gross lesions and scoring of its Training on the job, Central Veterinary Research Institute, Lusaka, Zambia SENEGAL Indirect and Direct CBPP diagnostic tests Training on the job, CBPP OIE Reference Laboratory c/o IZSAM, Teramo, Italy TURKEY Indirect and Direct CBPP diagnostic tests. Disease control strategy. Training on the job, CBPP OIE Reference Laboratory c/o IZSAM, Teramo, Italy KENYA CBPP gross lesions score. Participation to a Workshop organised by GALVmed as OIE expert TURKEY Use of the slaughterhouse as epidemiological monitoring of CBPP;Gross lesions description Classroom activity, Pendik-Turkey ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities,

6 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Mmm Proteins Identification 3 years Isolation, characterization & production of synthetic immunogenic membrane proteins CVRI-Lusaka Studies on Pathogenesis of Mmm 3 years Study of first stage of inflammatory reactions using nasal mucosae, trachea and lung explants. CVRI-Lusaka, KALRO- Nairobi, GALVmed- Edinburgh, Veterinary School-Teramo Studies on Pathogenesis of Mmm 3 years Role of host immune response in the pathogenesis of Contagious Bovine Pleuropneumonia using immunohistochemistry and confocal laser scanning microscopy CVRI-Lusaka, Veterinary School-Teramo Studies on Pathogenesis of Mmm 3 years Study of interaction between Mmm and host cells using OMIC approach CVRI-Lusaka, Veterinary School-Teramo CBPP gross lesions; new score system 2 years Develop a new scoring system concerning CBPP gross lesions CVRI-Lusaka, KALRO- Nairobi, GALVmed- Edinburgh, Veterinary School-Teramo OIE Twinning project 3 years Training on direct, indirect diagnosis and epidemiology of CBPP Pendik Institute TURKEY ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 2 1. Luciani M., Armillotta G., Manna L., Ciarelli A., Di Febo T., Krasteva I., Pini A., Sacchini F., D Angelo A.R. 6 OIE Reference Laboratory Reports Activities, 2016

7 Selection of a monoclonal antibody by ELISA, immunoblotting and Quartz Crystal Microbalance technology for immunohistochemical detection of Mycoplasma mycoides subsp. mycoides. Veterinaria Italiana, doi: /VetIt , available on line: Jasini A. Musa, James O.O. Bale, Haruna M. Kazeem, Nicholas D. Nwankpa, Andrea Di Provvido, Flavio Sacchini, Katiusia Zillia, Anna Abass, Massimo Scacchia, Attilio Pini;Molecular detection of Nigerian field isolates of Mycoplasma mycoides subsp. mycoides as causative agents of Contagious bovine pleuropneumonia International Journal of Veterinary Science and Medicine b) International conferences: 0 c) National conferences: 1 Giovanni Di Teodoro, Anna Rita D Angelo, Andrea Di Provvido, Gianluca Orsini, Gaetano Federico Ronchi, Massimo Scacchia, Giuseppe Marruchella.: Explants from bovine airways: a suitable tool to investigate the early pathogenesis of contagious bovine pleuropneumonia?; LXX SISVET Congress, June 2016, Palermo, Italy. d) Other: (Provide website address or link to appropriate information) 1 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 0 b) Seminars: 1 c) Hands-on training courses: 2 d) Internships (>1 month): 1 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training No. participants from the corresponding country b Turkey 80 c Zambia 12 c Turkey 1 d Senegal 1 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? OIE Reference Laboratory Reports Activities,

8 Quality management system adopted Certificate scan (PDF, JPG, PNG format) ISO https zmail.izs.it_service_home_~ auth=co&loc=en_gb&id=264877&part=2.pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited Complement Fixation test (CFT) Immunoblotting PCR-RFLP Accreditation body ILAC (Accredia) ILAC (Accredia) ILAC (Accredia) 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? National/ International Title of event Co-organiser Date (mm/yy) Location No. Participants International OIE Twinning Project PVCI Pendik, Turkey 2016 Teramo-Italy and Pendik-Turkye Did your laboratory participate in scientific meetings on behalf of the OIE? 8 OIE Reference Laboratory Reports Activities, 2016

9 Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented Use of the slaughterhouse as epidemiological monitoring of CBPP 04/16 Pendik-Turkey Speaker CBPP gross lesions description Workshop on CBPP Research Activities founded by GALmed 06/16 Nairobi-Kenya Speaker 1.CBPP gross lesions description 2.Lesion score system proposal ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant) No. participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. CFT Participant 8 Molecular Diagnosis Participant 4 Organising by Botswana CBPP OIE Ref Lab Organising by Botswana CBPP OIE Ref Lab 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? OIE Reference Laboratory Reports Activities,

10 Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories SADC CBPP Sub-Regional Scientific Network Disease control and standardization of diagnostic methods in SADC Region Botswana CBPP OIE Ref Lab Scientific coordinator ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? No Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) CBPP OIE ad hoc Group OIE Headquarter Group of CBPP experts 25. Additional comments regarding your report: -An OIE Twinning project on CBPP between IZSAM, as Parental Laboratory and Pendik Veterinary Control Institute, Turkey as candidate laboratory, started in 2015.In 2016 the OIE expert dr Scacchia visited Pendik Institute checking the technical activities carried out in Turkey after the training periods made in Teramo.One training period on diagnostic methods applicable to CBPP was carried out in 2016 in Teramo; - The OIE Reference Laboratory is involved in research projects sponsored by GALVmed on Vaccine efficacy and Antibiotic therapy. 10 OIE Reference Laboratory Reports Activities, 2016