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1 This Conference brought to you by: Linkedin/Groups: Technology Training Corporation
2 Instrument Performance & FBI RDNA Enhancement of CODIS Biometrics: Big Data, Analytics & Biometric Enable Intelligence December 13, 2013 Jennifer Wendel FBI Laboratory
3 Rapid DNA Analysis (Law Enforcement Rapid DNA) 3 Rapid DNA describes the fully automated t (hands free) process of developing an STR profile from a reference sample buccal swab. The swab in profile out process consists of automated extraction, ti amplification, separation, detection ti and allele l calling without human intervention. NO RAPID DNA INSTRUMENTS OR KITS HAVE BEEN APPROVED BY THE FBI FOR SUBMISSION OF SAMPLES TO NDIS/CODIS 2
4 Supreme Court of the United States Decision: Maryland v. King, 133 S. Ct (2013) 5-4 Decision: Collection of DNA from Arrestees is constitutional DNA identification of arrestees is a reasonable search that can be considered part of a routine booking procedure. 3
5 CODIS Architecture of NDIS NDIS SDIS SDIS SDIS LDIS LDIS LDIS Ft. Lauderdale Tallahassee Tampa 4
6 CODIS Integration of Rapid DNA (RDIS) NDIS SDIS SDIS SDIS LDIS LDIS LDIS Ft. Lauderdale Tallahassee Tampa RDIS Rapid DNA Index System (RDIS) 1,000 s LE Agencies involved in R-DNA 5
7 CODIS and Rapid DNA Integration 7 (Vision) The swab in profile out integration of Rapid DNA into CODIS requires the development of the Rapid DNA Index System (RDIS). RDIS consists of a two hour, two step process. The first step involves developing a CODIS DNA profile from an arrestee in under one hour and then searching the National DNA Index System (NDIS) during the second hour. RDIS Integration Phases Phase 1: Rapid Enrollment (Portal) Expand NDIS registration beyond 54 SDIS Phase 2: Rapid Hit Notification - Notification to the booking station and the investigating agency 6
8 Future Rapid DNA Workflow in the Booking Station CODIS SDIS SDIS SDIS SID# LDIS LDIS LDIS National DNA Database Hierarchy 7
9 Rapid DNA - High Level Portal Concept Booking DNA Collection LiveScan 1 Send PII Criminal 2 Identity Verified Receive Message: Collect DNA Biometric Identification Criminal History Record DNA in CODIS SID DNA Profiling 3 Send DNA Profile CODIS 8
10 Rapid DNA Hit Notification CODIS DNA Profile Search Forensic Forensic DNA DNA Notification Contact RDNA Hit Notification Invest. Agency/POC Incident# Offense Code Date Record Entered SID Notify VIA NGI HIT Investigating Agency Back up Notification Forensic DNA Laboratory RDNA Hit Notification Booking Agency Arresting Agency Date Record Entered SID# RDNA Hit Notification Booking Agency Arresting Agency Date Record Entered SID# NGI/CODIS Message Manager 9
11 RDNA Instrument Status IntegenX - RapidHIT September delivery and operation NetBio - ANDE (DNAscan) September delivery and operation Success is defined as concordant CODIS 13 loci results between R-DNA systems and current laboratory bench top genotyping techniques 10
12 Heat Maps for Sample Tracking 80% Correct Loci Calls 37% Correct CODIS Core 13 Loci Each cell represents a comparison between the known genotype and the R-DNA generated genotype Correct Call One of 2 Alleles Present No Alleles Incorrect Alleles Present Drop-In Present Heat map definitions agreed up by NIST, FBI, and DFSC 11
13 13 12
14 13
15 15 14
16 NetBio ANDE/DNAscan Instrument D3S1358 TH01 D21S11 D18S51 Penta_E D5S818 D13S317 D7S820 D16S539 CSF1PO Penta_D AMEL vwa D8S1179 TPOX FGA 15,18 9,9.3 29,29 12,13 10,17 11,11 11,11 8,13 10,11 10,13 9,12 X,X 14,18 12,15 8,11 20,
17 ANDE Sample Observations Drop-in (24.3,27) Drop-out (10,13) Alleles Present but not transferred to.cmf 16
18 18 17
19 18
20 20 19
21 IntegenX RapidHIT 200 Instrument D3S1358 TH01 D21S11 D18S51 Penta_E D5S818 D13S317 D7S820 D16S539 CSF1PO Penta_D AMEL vwa D8S1179 TPOX FGA 15,18 9,9.3 29,29 12,13 10,17 11,11 11,11 8,13 10,11 10,13 9,12 X,X 14,18 12,15 8,11 20,
22 RapidHIT 200 Sample Observations Artifacts Present Drop-in (13,14) Drop-out (8,11) Additional Alleles Present but not transferred to.cmf 21
23 R-DNA Instrument Demonstrations FBI Biometric Steering Committee Demo, Oct 2012: ANDE and RH200 each run twice during meeting 13/20 Samples 65% Success SWGDAM Demo, July 2013: 40 attendee samples each run on ANDE and RH /80 Samples 88% Success 22
24 NIST Interlaboratory Testing Each participant sent a standard sample set for testing Provide participants and sponsor with data and feedback Each participant will receive their specific performance feedback The sponsor (FBI) will get a cumulative report for dissemination Data and results will assist ongoing developmental validation studies and other decisions in the adoption of R-DNA Lab 1 NIST Inter-laboratory Samples (50 Buccal Swabs) Se end out sa amples NIST DoD DFSC FBI Data a returned to NIST Testing R-DNA Instrument(s) NIST Analyzed Results Res sults disse minated Lab 2 Lab 3 Cumulative Report to Sponsor (FBI) 24
25 R-DNA Instrument Status NIST Interlaboratory Study 50 samples (5 replicates of 10 unique individuals) Run on ANDE (4 instruments) and RH200 (3 instruments) Combined R-DNA Results: 309/350 Samples 88% Success 25
26 NETBIO ANDE/DNAscan SYSTEM Event Timeline Sept Delivery 37% CODIS 13 success November 2012 Jan 2013: Instrument idle Jan 2013 April 2013: Instrument returned to NetBio for upgrades April July: Instrument back to FBI 74% CODIS 13 success Average success remained the same August Present: Instrument returned to NetBio for software upgrades 26
27 IntegenX RapidHIT 200 SYSTEM Event Timeline Sept 2012: Delivery 39% Success November 2012 Jan 2013: Instrument idle Feb July 2013: Series of upgrades/pms Average success remained the same ~ 75% Success July 3rd Present: Instrument returned to IntegenX for software and hardware upgrades to include heated lysis 27
28 ANDE (NetBio) Timeline of Testing 100% Success = correct CODIS profile 90% 80% n=12 70% 60% 50% 40% 30% n=29 Instrument and biochips returned to NetBio for upgrades. n=17 System and software upgraded n=18 Software Up pgrade 20% 10% 0% n=14 n=3 NIST/DHS instrument n=93 chips (465 samples) tested from 9/20/2012 to 8/12/
29 RapidHIT 200 (IntegenX) Timeline of Testing 100% Success = correctcodis profile 90% 80% 70% 60% 50% 40% 30% 20% 10% n=12 Instrumen nt Repair n=8 n=32 n=41 Cap pillary needed d to be cleane ed n=23 Instru ment upgrad ded and repla aced n=27 0% NIST/DHS instrument n=143 chips (715 samples) tested from 9/20/2012 to 8/12/
30 R-DNA Instrument Performance 1. Spiral improvement of Prototypes t during first year 2. Allele calls are correct 3. Failed samples due to partial profile Software Drop out Chip/Cartridge failure 4. When the prototype machines work, they work well If sustained, success levels l are high h enough to perform developmental validation studies once hardware and software versions are finalized 30
31 For Additional Information Rapid DNA FAQs b SWGDAM us/lab/codis/codis-and-ndis-fact- sheet
32 Acknowledgements NIST Peter Vallone Erica Butts FBI Lilly Moreno DoD DFSC Brigid O Brien Karen Olson Rick Tontarski Jeff Salyards DHS Chris Miles Jennifer.wendel@ic.fbi.gov 33
33 Thank You Jennifer Wendel Chief, CODIS Unit
34 Vision of R-DNA Integration SWGDAM NDIS Today Industry R & D Laboratory FBI/NIST/LE Laboratory Performance Testing State Offender Concordance Validation SDIS State/Local Laboratory Reference Sample Use Police Booking Stationti Pilots Rapid DNA Task Force- APB
35 Rapid DNA Integration RDIS Rapid DNA Index System SDIS CODIS SDIS SDIS LDIS LDIS LDIS CJIS Advisory Policy Board (APB) Rapid DNA Task Force 1,000 s RDIS RDIS# RDIS#: ORI/RMac Model, Ser#/Oper/Agency Conf#/DRE/SID ISP-CHI/ANDE Pro, /LAM1/ISP / /IL
36 Rapid DNA Program Office Collaboration and Coordination Rapid DNA Task Force (CJIS APB, Identification Services Subcommittee) composed of DNA, criminal history, and identification systems SMEs; plan future workflows and requirements for Rapid DNA implementation Scientific Working Group on DNA Analysis Methods DNA SMEs who develop standards for forensic DNA analysis; will publish standards for validation and operation of Rapid DNA systems Rapid DNA User s s Group Rapid DNA machine users from Federal, State and Local forensic laboratories; share data generated from prototype instruments and perform controlled studies on collaborative sample sets CJIS Biometric Center of Excellence provided initial funding and for procurement of prototype instruments
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