Gene Expression Analysis Superior Solutions for any Project

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Gene Expression Analysis Superior Solutions for any Project Find Your Perfect Match ArrayXS Global Array-to-Go Focussed Comprehensive: detect the whole transcriptome reliably Certified: discover

1 Gene Expression Analysis Superior Solutions for any Project Find Your Perfect Match ArrayXS Global Array-to-Go Focussed Comprehensive: detect the whole transcriptome reliably Certified: discover exceptional data quality with validated Agilent microarrays All-rounder: available for numerous species Focussed: concentrate on relevant pathways and genes Flexible: choose between preconfigured and custom Carefree: get statistically analysed data from tissue samples MyArray Custom-made RT-qPCR Total confidence Unlimited: discover the advantages of microarrays for any species Reliable: trust in our experience from + projects Optimised: best performing probes guarantee high data quality Validated: get results with highest precision Fast: express track for data delivery within 3 days Individual: analyse any region or species

CHOOSE YOUR PERFECT MATCH EXPERIENCE THE DIFFERENCE Superior solutions for your project: OakLabs flexible gene expression profiling services guarantee rapid progress for your research.

2 CHOOSE YOUR PERFECT MATCH EXPERIENCE THE DIFFERENCE Superior solutions for your project: OakLabs flexible gene expression profiling services guarantee rapid progress for your research. OakLabs services provide you with high quality gene expression data which stand out from comparable products. Thus, you can make the most out of your research. Flexibility in Every Stage: From Identification of Candidate Genes to Verification of Biomarkers Missing Genes? An Issue of the Past To prove the high quality of our gene expression products we have conducted comparisons with competitor Genome-Wide Analysis of Representative Samples Focus the Analysis on Relevant Pathways in More Samples Verification of a few Genes in Some Samples products. See how they perform using the example of ArrayXS: Frequency ArrayXS Competitor log signal competitor s labelling 4 3 Signal Intensity log signal 3 4 log signal WT labelling Detectable transcripts Figure depicts the log probe signals of ArrayXS (blue) and its competitor (grey) for the same sample and demonstrates clearly that more genes can be measured on ArrayXS. Analysis of non-coding RNAs Obtain differentially expressed gene sets relevant for your study Gain deep insight into biological processes with a broadened experimental setup and sound statistics Rely on your findings due to valid-proof data Whole transcript (WT) labelling allows the detection of transcripts without poly(a) tail in addition to protein coding transcripts. Figure shows a scatter plot from signals of a sample labelled in two ways: WT labelling (x-axis) and competitor s labelling (y-axis). Genes whose activity is equally detected appear close to the diagonal line. The green area depicts transcripts exclusively measured in the WT labelled sample, indicating the gain of information with ArrayXS combined with WT labelling. Performance of each individual probe Based on Your Needs Different probes of the same gene vary strongly in their signal intensities. Each gene on ArrayXS is represented by a probe that performed best in wet lab hybridisations according to several parameters. For a randomly picked gene, Figure 3 illustrates the higher signal obtained with the probe on ArrayXS (green shadowed) compared to the very low signal on the competitor s microarray (red shadowed) The focus of our order was to analyse the transcript abundances of plant-pathogenic fungi during the infection, and to compare wildtype vs. mutant. The quality of the results was excellent and highly reproducible. All our expectations were met and we would truly recommend OakLabs. We offer the perfect solution for each of your gene expression projects no matter if you want to analyse the complete transcriptome (ArrayXS), focus on relevant pathways (Array-to-Go) or validate candidates (RT-qPCR). For the development of a custom array design, our optimised MyArray strategy is the perfect solution. Your with OakLabs Services Prof. Dr. Holger Deising, Institute of Agricultural and Food Sciences, Martin-Luther-University Halle-Wittenberg, Germany Dedicated probe designs and protocols guarantee high quality data of superior sensitivity and precision Fast turnaround time of just two weeks allows you to move forward with your project quickly Our carefree packages include RNA isolation and sound data analysis and allow you to focus on your research Get advice from our experts and rely on the optimal solution for your project Comprehensive data analysis, individually adapted to your needs to unravel the secrets of your study rapidly

3 ArrayXS EXPERIMENTALLY VALIDATED MICROARRAYS Array-to-Go WHERE MICROARRAY S THROUGHPUT MEETS qpcr S SENSITIVITY ArrayXS microarrays provide high quality genome-wide gene expression data and are available for numerous species. Array-to-Go stands for comprehensive analysis of relevant genes of a pathway, a biological process or a disease. Enjoy the Focus Differential gene expression analysis without microarrays has become scarcely imaginable as they are valued for their robustness, reliability and fast data acquisition and analysis. Though there are microarrays available for a variety of different species, many of today s applications require novel arrays to detect the whole transcriptome comprehensively and sensitively. With our ArrayXS microarrays we provide experimentally validated Agilent microarrays that combine up-to-dateness, comprehensiveness and quality to create an outstanding R&D experience for a broad range of model species. Array-to-Go was developed towards highest sensitivity to compete with qpcr. A proprietary lab protocol is perfectly aligned to a proven probe design and Array-to-Go convinces customers worldwide. RT-qPCR experiments tend to become expensive. I was tired of analysing just a subset of relevant genes with qpcr panels. I was overwhelmed by RNA seq data. We ordered the service on ArrayXS Zebrafish to get some insights into the molecular mechanisms of the gene we are working on. We are impressed by the speed and quality of the service and very pleased with the results. For sure we will take advantage of OakLabs service again. I want to focus just on the genes involved in the disease I study. For high sensitivity RT-qPCR I need much more total RNA than available. Dr. Chris Jopling IGF - Institut de Génomique Fonctionelle, Montpellier, France Preconfigured or Custom - Choose your Optimal Compilation Parallel analysis of mrna and non-coding transcripts from total RNA without cutting back Accurate access to low-abundant transcripts and a dynamic range over five orders of magnitude Straightforward data acquisition and analysis for fast interpretation Compatible with our small sample protocol to profile single cells Available for the major model species for delivery or bundled with our -weeks comprehensive service With Array-to-Go you can either choose from currently more than 9 preconfigured compilations or simply have your Array-to-Go custom-tailored for any species with up to, of your genes of interest. Straightforward analysis of the most relevant up to, genes Fully customisable for any species without design fee Comprehensive service package from tissue samples to visualised data Superior sensitivity from just ng total RNA

4 MyArray ARRAYXS FOR ANYBODY RT-qPCR THE CHERRY ON TOP MyArray opens the door for the advantages of ArrayXS based gene expression profiling for any species: reliability, sensitivity, comprehensiveness and rapidity. The gold standard to validate large throughput data. The MyArray Workflow - Development of the XL microarray High density microarrays are used to represent every gene by numerous different probes. - Hybridisation of RNA and DNA samples Total RNA as well as genomic DNA samples are processed and hybridised onto the XL microarrays. 3 - Selection Based on the wet lab data and several parameters, the best probes are selected and represented on ArrayXS. Our Best Seller MyArray Projects Our leveraged RT-qPCR service starts at project consultancy, includes RNA isolation, assay design, validation and optimisation and ends with statistical analysis and a data report. Rely on our MIQE compliant RT-qPCR workflow Get data within weeks or even the day after tomorrow with our express track Standalone service or bundled as a carefree add-on with our large throughput solutions The available tools for expression profiling in my study species were outdated, a lot of genes were missing. Thanks to the MyArray Service I now can reliably profile even non-coding RNAs in addition to mrna. I work with several prokaryotic strains and needed a tool that allows to reliably measure gene expression in all strains of a species. I study host-pathogen interaction where the pathogen makes up a tiny portion of the sample. With RNA seq I needed a huge sequencing effort to get expression data for the pathogen with low sensitivity. Due to the MyArray service I now can measure gene expression in both species simultanously at high sensitivity. Guarantee for high quality gene expression data for any species Perfectly and individually tailored to your needs Flexible pricing for your project s budget My study species is not yet sequenced, nevertheless I now can take advantage of microarray-based gene expression analysis. Excellent consultation due to our experience gained in numerous projects GET EXACTLY THE DATA YOU NEED OakLabs services provide you with extensive data analyses which allow you to extract the needed information quickly. Our analysis portfolio includes: Data normalisations Analysis of significantly differentially expressed genes Outlier analysis Visualisation of relevant pathways Signal distributions Box plots Hierarchical clustering Heat maps Principal component analysis Correlation analysis Volcano plots

5 ORDERING INFORMATION PRODUCT ArrayXS * ** ArrayXS Arabidopsis ArrayXS C. Elegans ArrayXS Drosophila ArrayXS Human ArrayXS Human FFPE ArrayXS Mouse ArrayXS Rat ArrayXS Rice Indica ArrayXS Rice Japonica ArrayXS S. Scrofa ArrayXS Zebrafish ArrayXS Custom Array-to-Go Array-to-Go, preconfigured, S Array-to-Go, preconfigured, M Array-to-Go, preconfigured, L Array-to-Go, preconfigured, XL Array-to-Go, custom, S Array-to-Go, custom, M Array-to-Go, custom, L Array-to-Go, custom, XL MyArray MyArray Platin MyArray Plus MyArray Bacteria Best Choice MyArray Bacteria Plus MyArray Bacteria Tiling MyArray Seq RT-qPCR RT-qPCR TaqMan RT-qPCR SYBR Green NO. OF GENES MIN SAMPLES DETAILS ORDER NO. 3,73 7,9 9,94 9, 33,97,37 4,9 4,,7 9,9,3 up to, genome build TAIR-9 genome build WormBase WS, validated for whole transcript profiling genome build R.4, validated for whole transcript profiling genome build GRCh3, validated for whole transcript profiling genome build GRCh3, probe design and protocol adapted to FFPE samples genome build GRCm39.p, validated for whole transcript profiling genome build Rnor., validated for whole transcript profiling genome build BGI genome build RGAP7 genome build Sscrofa., validated for whole transcript labelling genome build Zv9, release 7, validated for whole transcript profiling outcome of a MyArray project, available for any species XS- XS- XS- XS- XS- XS- XS-3 XS-7i XS-7j XS-9 XS-4 XS- < AG-S-X-X -, AG-M-X-X, -, AG-L-X-X > AG-XL-X-X < AG-S-XX- -, AG-M-XX-, -, AG-L-XX- >, AG-XL-XX- up to, 3 for sequenced species MY--P up to, 3 validated for multiple races or ecotypes MY--X up to, seq. for sequenced prokaryotes MY--A up to, seq. universal design validated for multiple strains MY--B up to 4, seq. expression analysis including intergenic and antisense transcriptional activity MY--C up to, seq. 4 for species with limited sequence information MY--S up to 3 validated probes for most genes in many model species, express track available RT-X-A up to flexible service option, express track available RT-X-B * Your study species does not appear in the list? Contact us, an ArrayXS might be in development. ** All ArrayXS microarrays are in xk format, subarrays/slide, contain spike-in control probes and min. 3 x replicates of biological probes. ArrayXS microarrays are compatible with - and -color hybridisations, best results are obtained in combination with Agilent's reagents and protocols as well as Agilent's SureScan Microarray Scanner. Get Your Individual Offer Today! service@oak-labs.com +49() 33 / OakLabs GmbH Neuendorfstraße B 7 Hennigsdorf Germany

Gene expression microarrays and assays. Because your results can t wait

Gene expression microarrays and assays Because your results can t wait A simple path from data to decision-making The power of expression microarrays Transcriptome-wide analysis can be complex. Matching