DR. SIDRA REHMAN. Molecular Biology, Ph.D.
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1 PERSONAL BIODATA DR. SIDRA REHMAN Molecular Biology, Ph.D. Marital Status: Married CURRENT STATUS Assistant Professor: Aug - To date (TTS) Department of Biosciences COMSATS Institute of Information Technology, Islamabad Campus, Pakistan HIGHPOINTS OF EDUCATION Goal oriented Researcher with molecular biology, biotechnology, molecular virology, cancer genomics, organic chemistry, natural products research background and education. I would like to use acquired teaching and research experience with the aim towards professional and research growth of the institute. PREVIOUS JOB EXPERIENCE Assistant Professor: Aug July (TTS) (Biotechnology Program) Department of Environmental Sciences COMSATS Institute of Information Technology, Abbottabad Campus, Pakistan Assistant Professor: July 2014-June 2015 (Contract) Allama Iqbal Medical College and Jinnah Hospital Complex, Lahore, Pakistan Optimization of in vitro assay for analysis of anti-proliferative potential of plants extracts against Estrogen dependent Breast Cancers Research Associate: January 2014-June 2014 Allama Iqbal Medical College and Jinnah Hospital Complex, Lahore, Pakistan. Purification of anti-hcv compound from Medicinal Plants 1
2 Development of in vitro assay for analysis of anti-proliferative potential of plants extracts against Estrogen dependent Breast Cancers Research Responsibilities: Conducted research on extraction and purification of medicinal plants using column chromatography, TLC and HPLC. Studied the effect of medicinal plants and their purified fractions against HCV genes as well as whole virus. Worked on screening of medicinal plants against Estrogen dependent Breast Cancer. Involvement in lab management. Engrossment in resolving troubleshooting for optimizing research experiments. Contribution in maintenance of standard operational procedures. Provide guidance to students in conducting experiments. TEACHING EXPERIENCE Teaching Courses (BS): General Virology Molecular Genetics Health Biotechnology Principles of Virology Teaching Course (PhD): Cell and Tissue Culture Marine Biotechnology ADMINISTRATIVE RESPONSIBILITIES Current Administrative Duties: Member of Graduate Committee Previous Administrative Duties: Departmental Operating Officer (DOO) of Biotechnology Program (01y01m20d) Member of department admission committee Class Advisor Class Advisor Member of Department Academic Review Committee (DARC) Member of Campus Academic Review Committee (CARC) RESEARCH STUDENTS SUPERVISED 2
3 03 BS Students (Completed) 01 MS Student (Completed) # Student Names Thesis Titles BS/MS/ PhD 1. Mubashar Khan Molecular and Mechanistic Studies of Selected Medicinal Plants for Hepatitis C Virus Treatment by Targeting its Core Gene 2. Bibi Maryam MAX Expression Analysis of Human Biopsy Samples of Prostate Cancer (PCa) 3. Zain Asif Alvi WEE1 Expression Analysis of Human Biopsy Samples of Prostate Cancer 4. Hamza Khan Comparison of IL-8 expression pattern among peg- Interferon and Sofosbuvir treated hepatitis C patients Supervisor / Co- Supervisor Status: Registered / Completed MS Supervisor Completed BS Supervisor Completed BS Supervisor Completed BS Supervisor Completed EDUCATION Mphil Leading to PhD (Molecular Biology) National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan. Bioassay Guided Screening and Purification of Indigenous Plants against HCV NS3 Protease. M.Sc (Chemistry) Institute of Chemistry, University of the Punjab, Lahore, Pakistan. Synthesis and Characterization of Schiff Bases derived from Aromatic Aldehydes 3
4 B.Sc (Botany, Zoology, Chemistry) University of the Punjab, Lahore, Pakistan. F.Sc (Pre-Medical) Higher Secondary School Certificate Examination, FBISE, Islamabad, Pakistan Matric (Science) Secondary School Certificate Examination, FBISE, Islamabad, Pakistan INTERNATIONAL PUBLICATIONS (Impact Factor: ) 1. Waqar Ahmad, Bushra Ijaz, Khadija Shabbiri, Fayyaz Ahmed, Sidra Rehman. Oxidative toxicity in diabetes and Alzheimer's disease: mechanisms behind ROS/ RNS generation. Journal of Biomedical Science. 24: 76 (). IF: Sidra Rehman*, Bushra Ijaz, Nighat Fatima, Syed Aun Muhammad and Sheikh Riazuddin. Therapeutic potential of Taraxacum officinale against HCV NS5B polymerase: In-vitro and In silico study. Biomedicine & Pharmacotherapy 83: (2016) IF: Muhammad Dawood, Nighat Fatima, Amara Mumtaz, Sidra Rehman, Irum Shazadi, Qaisar Mahmood and Syed Aun Muhammad. Molecular Docking Studies of Sesquiterpenoids against Helicobacter pylori Peptide Deformylase. BJPR 10(3):1-7 (2016) ISI 4. Sidra Rehman, Usman A Ashfaq, Sana Riaz, Tariq Javed and Sheikh Riazuddin. Antiviral activity of Acacia nilotica against Hepatitis C Virus in liver infected cells.virol J 8(1):220 (2011) IF: Sidra Rehman, Usman A Ashfaq and Tariq Javed. Antiviral drugs against Hepatitis C Virus. Genet Vaccines Ther 9(1):11 (2011) (ISI) 6. Usman A Ashfaq, Tariq Javed, Sidra Rehman, Zafar Nawaz and Sheikh Riazuddin. Inhibition of HCV 3a core gene through Silymarin and its fractions. Virol J 8 (1): 153 (2011) IF: Usman A Ashfaq, Tariq Javed, Sidra Rehman, Zafar Nawaz and Sheikh Riazuddin. Lysosomotropic agents as HCV entry inhibitors. Virol J 8 (1): 163 (2011) IF: Tariq Javed, Usman A ashfaq, Sana Riaz, Sidra Rehman. In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus. Virol J 8(1): 26 (2011). IF:
5 9. Usman A Ashfaq, Muhammad Ansar, Muhammad Tahir Sarwar, Tariq Javed, Sidra Rehman and Sheikh Riazuddin. Post-transcriptional inhibition of Hepatitis C Virus replication through small interference RNA. Virol J 8 (1):112 (2011). IF: Usman A Ashfaq, Tariq Javed, Sidra Rehman, Zafar Nawaz and Sheikh Riazuddin. An overview of HCV Molecular biology, replication and immune responses. Virol J 8 (1): 161 (2011). IF: Muhammad Ansar, Usman A Ashfaq, Imran Shahid, Muhammad T Sarwar, Tariq Javed, Sidra Rehman, Sajida Hassan and Sheikh Riazuddin. Inhibition of full length Hepatitis C Virus particles of 1a genotype through small interference RNA. Virol J 8 (1): 203 (2011). IF: Accepted Articles: Nil SCIENTIFIC SYMPOSIA AND CONFERENCES ATTENDED14 th th -28 th August 2016: 13 th Biennial Conference on Recent Advances & Challenges in Molecular Biology, Biochemistry and Biotechnology at COMSATS Institute of Information Technology, Abbottabad rd 25 th August 2015: 2 nd International Conference on Drug development Natural and Synthetic at COMSATS institute of Information Technology, Abbottabad th April 2015: Workshop on An Introduction to Next Generation Sequencing Platform & Its Application in Agricultural Sciences at Institute of Agricultural Sciences, University of the Punjab, Lahore th - 22 nd August 2013: Three days International Conference on Applications of Molecular Biology in Medicine & Agriculture conducted by Department of Biochemistry, Quaid-i- Azam University, Islamabad th March 2012: One day symposium on Applications of Biotechnology in health and Agriculture held at Department of Bioinformatics & Biotechnology, Government College University, Faisalabad th November 2011: One day workshop on young researchers skill development held at National University of Science and Technology (NUST), Islamabad th -28 th March 2009: National Bioforum 2008 organized and attended symposia in Bio Regarding Molecular Biological Research, sirna technology as therapeutic tool and a workshop on regulation of use of GM crops. National Centre of Excellence in Molecular Biology (NCEMB), Lahore, Pakistan. PRESENTATIONS DELIVERED IN CONFERENCES4 th 5
6 23 rd 25 th August 2015: Oral presentation was delivered on Estrogen Dependent Breast Cancers in 2 nd International Conference on Drug development Natural and Synthetic at COMSATS Institute of Information Technology, Abbottabad. RESEARCH PROJECTS GRANTS 1. HEC-Startup Grant titled, Molecular and mechanistic studies of selected medicinal plants for the treatment of Hepatitis C virus by inhibiting core gene expression of genotype 3a. An amount of 0.5 million, Project # 830 has been granted. (Completed, Duration: 28 April April ). MOLECULAR BIOLOGY EXPERTISE Cell Culturing (Mammalian cell culture) and related operations such as passaging cultures and cryopreservation. Toxicological and proliferation assays. In-vitro Transfection and infection assays. RNA Extraction, Nano drop RNA quantification. cdna synthesis, PCR amplification, Electrophoresis. Real-time PCR Protein purification, SDS-PAGE, Western Blotting. Molecular Cloning Techniques. Mini & Maxi Prep for plasmid purification. Regulation of Gene Expression. OTHER RESEARCH TECHNIQUES Chemistry related Techniques e.g. Compound analysis, titrations, Chromatography (TLC, Column Chromatography, HPLC). Microbiology Techniques (Disinfections and sterilization, Lab safety, Specimen collection and processing, Smear examination, Cultures and isolation of microbes, Biochemical examination, Cell culturing etc). BIOINFORMATICS SKILLS Graphics Coral Draw, Chem Draw, Adobe Photoshop Statistical Analysis SPSS, Graph pad prism, Microsoft excel Genome Browser NCBI, UCSC, Proteome Browser PDB, Swiss Port Sequence Analysis Blast, Clustal W Primer Designing Primer 3 COURSES STUDIED IN M.Phil AND PhD 6
7 Molecular Biology. Biotechnology. Recombinant DNA techniques, Gene Expression and Regulation. Bioinformatics (DNA & protein software), Biostatistics and SPSS. Molecular Virology, Cell biology. Plant molecular biology. Medical genetics, stem cells. Genetically Modified Organisms (GMOs), Real-time PCR. IT ENVIRONMENT PROFICIENCY Operating system Windows 7 Windows 98, XP Web Tools HTML Microsoft Front page Databases Microsoft Access Multimedia Adobe Photoshop Adobe Flash Others: Microsoft Office 2007/2010(Word, Excel, PowerPoint) Windows Software Installation & Troubleshooting. PC Security Management CERTIFICATES Two merit certificates and scholarship from POF board and two Qirat competition certificates. Merit HEC Indigenous Scholarship for PhD OTHER INTERESTS Books Reading and Cooking Internet Browsing Painting and interior decoration 7
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