Your expert around cell culture. Serum-Free Media
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1 Your expert round cell culture Serum-Free Medi
2 Cell culture goes vegn! Overview pge 3 Serum substitutes pge 4 Medi for lymphocytes pge 5 Medi for stem cell culture pge 6 Other cell specific serum-free medi pge 7
3 OVERVIEW Serum-free cell medi hve incresingly become n importnt but chllenging topic for mny cell products nd cell cultivtion processes. With more thn 16 yers of professionl experience in providing serum-free solutions for customers worldwide, PAN-Biotech hs developed product portfolio of more thn 450 diferent medi products for wide rnge of cell cultures. Figure 1: PAN-Biotech offers brod rnge of serum-free medi products in different ctegories. Customers of PAN-Biotech re not only the users of our products with high-qulity stndrds but lso become prt of our network of brillint experts in stem cell reserch, tissue engineering, immunologicl therpy, toxicology nd mny other. Advntges Free from niml derived components Complince with legl requirements for the mnufcture of medicl products Complince with legl requirements for importing biotechnologicl products Lower risk of contmintion Reproducibility Costly btch testing cn be dispensed Simpliied downstrem processes Defined formultion Figure 2: Qulity specifictions of serum-free cell culture medi. 3
4 SERUM SUBSTITUTES Use Pnexin to develop your own serum-free medi. he serum substitutes (Pnexin) developed by PAN-Biotech cn be used for mny cell lines to replce FBS. Due to selected nd pretested rw mterils Pnexin btches re very homogeneous. herefore the complex btch testing known from FBS cn be omitted with the use of Pnexin. In ddition there is no need to chnge the previously used bsl medium. Pnexin is completely fully deined nd contins no growth fctors, undeined peptones or hydrolystes. Medium Suitble Cells Avilbility SFM PF ADCF HDCF FD Pnexin NTA Wide rnge of dherent cells Pnexin NTS Wide rnge of suspension cells Pnexin NTA Phrm Grde Pnexin NTS Phrm Grde Pnexin BMM Wide rnge of dherent cells Wide rnge of suspension cells Mouse bone mrrow mcrophges SFM: Serum Free Medium PF: Protein Free ADCF: Animl Derived Components Free HDCF: Humn Derived Components Free FD: Fully Deined Figure Efficiency nd growth stimultion of Pnexin NTA in DMEM/F12 compred to FBS in different cell lines 10% FBS 10% Pnexin NTA Cell number fter Dy 7 (x1000 Cells(cm²) L929 CHO HAT-29 HEK293 MDCK 3T3A 4
5 MEDIA FOR LYMPHOCYTES With the immunologicl cell therpy moving to commerciliztion the demnd of sclble production of immune cells lso incresed signiicntly. PAN-Biotech hs developed wide rnge of serum-free medi for the efective in vitro expnsion of immune cell cultures. Medium Suitble Cells Avilbility SFM PF ADCF HDCF FD Pnserin 413 T cells, NKT nd CIK cells Pnserin 413 Phrm Grde T cells, NKT nd CIK cells Pnserin 416 Dendritic cells Pnserin 416 Phrm Grde Dendritic cells Coming soon! Coming soon! Pnserin 701 Lymphocytes Pnserin 411 Myeloid/lymphoid cells And more! SFM: Serum Free Medium PF: Protein Free ADCF: Animl Derived Components Free HDCF: Humn Derived Components Free FD: Fully Deined Pnserin 413 with 5% plsm Pnserin 413 Expnsion folds Dy 0 Dy 3 Dy 7 Dy 9 Dy 11 Dy 14 Figure 4: It shows expnsion folds of Pnserin 413 with 5% plsm in comprison to Pnserin 413 within 14 dys. 5
6 MEDIA FOR STEM CELL CULTURE Stem cell medi free of niml-derived components PAN-Biotech ofers brod rnge of serum-free medi for stem nd progenitor cells in the most importnt ields of reserch nd development. Most of the medi re developed free of niml-derived components to meet the demnd of clinicl pplictions, especilly with respect to regenertive medicine, toxicology nd dignostics. Medium Suitble Cells Avilbility SFM PF ADCF HDCF FD PowerStem HE Humn embryonic stem cells PowerStem ips PowerStem MSC PowerStem HPSC Humn induced pluripotent stem cells Humn mesenchyml stem cells Humn hemtopoetic stem cells PowerStem PEC Humn ngiogenetic stem cells And more! SFM: Serum Free Medium PF: Protein Free ADCF: Animl Derived Components Free HDCF: Humn Derived Components Free FD: Fully Deined JM8 / BlckSix Cells in PowerStem ESPro1 B6N-mES-Cells in PowerStem ESPro2 H9-hES-Cells in PowerStem HE1 hes3-hes-cells in PowerStem HE2 Hemtopoetic stem cells from neontl cord blood in PowerStem HPSC hu-bm-msc in PowerStem MSC hu-outgrowth-epc in PowerStem PEC Figure 5: Microscopicl pictures of different stem cell cultures. 6
7 OTHER CELL SPECIFIC SERUM-FREE MEDIA PAN-Biotech lso produces mny other cell speciic serum-free medi for toxicology, tissue engineering, industril vccine nd ntibody productions. Medium Suitble Cells Avilbility SFM PF ADCF HDCF FD Endopn 300SL Humn endothelil cells Endopn 300SL Phrm Grde Humn endothelil cells Coming soon! Neuropn Neuronl cells Pnserin 293S HEK-293 (suspension) Pnserin 401 Wide rnge of diferent cells Pnserin 801 Humn kertinocytes Pnserin 801 Phrm Grde Humn kertinocytes Coming soon! Pnserin C6000 CHO (suspension) Pnserin H4000 Hybridom (suspension) Pnserin ProVero Vero cells (dherent) Spodopn Insect cells (suspension) And more! SFM: Serum Free Medium PF: Protein Free ADCF: Animl Derived Components Free HDCF: Humn Derived Components Free FD: Fully Deined 7
8 PAN-Biotech GmbH Am Gewerbeprk 13 D Aidenbch GERMANY Phone: +49 (0)8543/ Fx: +49 (0)8543/
Your expert around cell culture. Serum-Free Media
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