Dongsheng Biotech Co., Ltd. was established in 2005 in Guangzhou China.

Transcription

1 Introduction Dongsheng Biotech Co., Ltd. was established in 2005 in Guangzhou China. We focus on R&D and produce fundamental molecular biology reagents. In the past 5 years, Dongsheng Biotech has successfully grown up from a start-up to a professional organization. Our products have worldwide sales and establish an excellent reputation. We were ISO 9001:2008 certificated by BSI. We are also authorized import and export manufacture in China. With professional R&D group and strict control of the production process, we now have customers all over the world. Our mission is to provide professional and high quality products for life science research. To make your research more professional and more accurate, we are always making our best efforts.

2 Contents DNA Laddes & Markers 5 1 PCR, qpcr & RT-PCR 20 2 DNA Purification Kits 38 3 RNA Purification Kits 46 4 Silica Spin Column 50 Ordering Information For technical and sales inquiries: Dongsheng Biotech Co., Ltd Tel: , Fax: order@dongshengbio.com Website:

3 Make Your Research More Professional More Accurate Dongsheng Biotech provides wide range of molecular biology products for life science research. Product Line: 1. DNA Electrophoresis Products 2. PCR Products 3. DNA Purification Products 4, RNA Purification Products 5. Other Associative Reagents. Key Products : Taq DNA Polymerases PCR Mix DNA Ladders PCR & Gel Extraction Kits TRAzol Additionally, all product provides custom synthesis.

4 Dongsheng Biotech Product Application area Serve three major customer groups: (1) Laboratory reagent kits producers, a smaller group of customers (<50 major customers worldwide) who purchase substantial quantities of nucleotide monomers as raw material for specialized laboratory kits for use in research laboratories. (2) Diagnostic kits producers who use our nucleotide monomers as diagnostic agents in hospitals, blood collection agencies and pathology laboratories primarily for amplification. (3) Research laboratories in universities, industries, governments and hospitals & medical schools.

5 DNA Ladders & Markers Product List 1 DNA Ladders & Markers Product name Cat # Range 10bp ladder 20bp ladder low ladder 50bp ladder 50bp ladder plus 100bp ladder 100 bp ladder plus 1kb ladder 1kb ladder plus 200 bp ladder 500bp ladder?dna/hind Marker 1 Marker 2 Marker 3 Marker 4 DS 2000 DS 5000 DS DS x Swift DNA Electrophoresis Buffe 50xTAE Buffer 10xTBE Gel Loading Dye(6x) Gel Loading Dye, Blue(6x) Gel Loading Dye, Three-colour (6x) Gel Loading Dye, Orange(6x) Gel Loading Dye, SDS (6x) DSView Nucleic Acid Stain Agarose M1011 M1012 M1021 M1022 M1031 M1032 M1041 M1042 M1051 M1052 M1061 M1062 M1071 M1072 M1181 M1182 M1191 M1192 M1151 M1152 M1251 M1252 M1201 M1202 M1081 M1082 M1091 M1092 M1121 M1122 M1231 M1232 M1101 M1102 M1111 M1112 M1221 M1222 M1161 M1162 M9011 M9021 M9031 M9041 M9051 M9061 M9071 M9081 M7011 N ml 500ml 500ml 500g bp bp bp bp 50-1,000bp 100-1,500bp 100-3,000bp ,000bp ,000bp 200-4,000bp 500-5,000bp ,130bp bp 100-1,200bp 200-4,500bp ,000bp 100-2,000bp 100-5,000bp ,000bp ,000bp 5 Tel:

6 1. DNA Ladders & Markers Fax:

7 DNA Markers and Ladders 1 DNA Ladders & Markers PCR and plasmid digestion by restriction enzymes are used for the production of DNA fragments for our ladders/markers. These products are stable during prolonged incubations at room temperature and multiple freeze-thaw cycles. Distinguishing features: Bright, sharp bands for highly accurate sizing Precise DNA concentration, quantitative High resolution, low-background Stable and uniform with different batches Storage Stable for 3 months at RT, 2 years -20 Guideline for DNA Electrophoresis 1. Electrophoresis gel 1.1 Agarose gel Table 1. Different kinds of Agarose for different size of DNA range. Agarose (%) Standard agarose High EEO agarose Low melt agarose Low melt low EEO agarose 1.2 SDS-PAGE gel SDS-PAGE gel (Polyacrylamide gel) is for separation of small size DNA range ( bp) Table 2. Different concentration of SDS-PAGE gel for different size of DNA range SDS-PAGE gel (%) Effective DNA range 100 bp~1 kb 80 bp~5 7 Tel:

8 DNA Ladders & Markers 2. Gel electrophoresis system 2.1 Electrophoresis buffer Table 3.Guideline for electrophoresis buffer Type Swift DNA elecctrophoresis buffer Feature High speed Application High voltage and quick electrophoresis Small amount but needs recovery DNA 1 TAE Buffer TBE Buffer 2.2 Electrophoresis condition Low buffer capability High speed for ds linear DNA High buffer capability Good DNA separation, but low recovery rate Recommend for genomic DNA Macromolecular super helical DNA DNA or RNA above 1500 bp DNA mobility in electrophoresis is effective by DNA size and structure, electrophoresis voltage, etc. Recommend for DNA or RNA below 1500 bp Table 4. Guideline for voltage and buffers for agarose gel electrophoresis DNA Laders & Markers DNA size 1 kb 1~12 kb 12 kb High throughput electrophoresis (~10kb) Voltage 5~10 V/cm 4~10V/cm 1~3 V/cm ~23 V/cm Buffer TEB or Swift DNA electrophoresis buffer TAE or TBE or Swift DNA electrophoresis buffer TAE or Swift DNA electrophoresis buffer Swift DNA electrophoresis buffer 3. Guideline for DNA Ladders choice order@dongshengbio.com Fax:

9 10 bp ladder Cat: M µg, M1012-5x50µg 1 DNA Ladders & Markers 10bp ladder M1011/M % SDS-PAGE bp ? 200? 190? 180? 170? 160? 150? 1? 130? 120? 110? 100? 90? bp ladder is ideal for determining the size of double-stranded DNA from 80 to 300bp base pairs. The ladder consists of 18 linear double-stranded fragments. The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 100bp and 200bp are ng. The 100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use. Concentration: 168ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 100ng/ l, Other bands: ng/ l Recommended Electrophoresis Condition 8cm 5% Agarose Gel, 0.5x TBE, 5V/cm, 1h 20cm 10% SDS-PAGE Gel, 1x TBE, 8V/cm, 3h Contents(bp) 80, 90, 100, 110, 120, 130, 1, 150, 160,170,180, 190, 200, 220, 2,260, 280, 300 Storage: 3 months at room temperature, 2 years at bp ladder Cat. # M µg, M1022-5x50µg 10% SDS-PAGE bp 300? 280? 260? 2? 220? 200? 180? 160? 1? 120? 100? 80? µl/lane, 8 cm gel 0.5 TBE, 5 V/cm, 1h 20bp ladder is ideal for determining the size of double-stranded DNA from 60 to 300bp base pairs. The ladder consists of 13 linear double-stranded fragments. The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 100bp and 200bp are ng. The 100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use. Concentration: 128ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 100ng/ l, Other bands: ng/ l Recommended Electrophoresis Condition 8cm 5% Agarose Gel, 0.5x TBE, 5V/cm, 1h 20cm 10% SDS-PAGE Gel, 1x TBE, 8V/cm, 3h Contents(bp) 60, 80, 100, 120, 1, 160, 180, 200, 220, 2,260, 280, 300 Storage: 3 months at room temperature, 2 years at Tel:

10 DNA Ladders & Markers 50 bp ladder Cat: M µg, M1042-5x50µg 3% agarose 3% agarose 50bp ladder is ideal for determining the size of double-stranded DNA from 50 to 500bp base pairs. The ladder consists of 8 linear double-stranded fragments. The 250bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 250bp is ng. The 250bp is 100ng. This ladder is pre-mixed with loading dye and is ready to use. Concentration: 76ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 100ng/ l, Other bands: ng/ l Recommended Electrophoresis Condition 8cm 3% Agarose Gel, 0.5x TBE, 5V/cm, 1h Contents(bp) 50, 100, 150, 200, 250, 300, 0,500 Storage: 3 months at room temperature, 2 years at bp ladder plus Cat. # M µg, M1052-5x50µg 50bp ladder plus is ideal for determining the size of double-stranded DNA from 50 to 1000bp base pairs. The ladder consists of 13 linear double-stranded fragments. The 250bp and 500bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 250bp and 500bp are ng. The 250bp and 500bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use. Concentration: 128ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 100ng/ l, Other bands: ng/ l Recommended Electrophoresis Condition 8cm 3% Agarose Gel, 0.5x TBE, 5V/cm, 1h Contents(bp) 50, 100, 150, 200, 250,300, 0, 500, 600, 700,800, 900, DNA Laders & Markers Storage: 3 months at room temperature, 2 years at order@dongshengbio.com Fax:

11 100 bp ladder Cat: M µg, M1062-5x50µg 1 DNA Ladders & Markers 1.7% agarose 100bp ladder is ideal for determining the size of double-stranded DNA from 100 to 1500bp base pairs. The ladder consists of 11 linear double-stranded fragments. The 500bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 500bp is ng. The 500bp is 100ng. This ladder is pre-mixed with loading dye and is ready to use. Concentration: 100ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 100ng/ l, Other bands: ng/ l Recommended Electrophoresis Condition 8cm 1.7% Agarose Gel, 0.5x TBE, 5V/cm, 1h Contents(bp) 100, 200, 300,0, 500, 600,700,800,900,1000,1500 Storage: 3 months at room temperature, 2 years at bp ladder plus Cat. # M µg, M1072-5x50µg 1.7% agarose 100bp ladder plus is ideal for determining the size of double-stranded DNA from 100 to 3000bp base pairs. The ladder consists of 14 linear double-stranded fragments. The 500bp and 1200bp fragment are present at increa sed intensity to allow easy identification. Al l fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 500bp and 1200bp are ng. The 500bp and 1200bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use. Concentration: 136ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 100ng/ l, Other bands: ng/ l Recommended Electrophoresis Condition 8cm 1.7% Agarose Gel, 0.5x TBE, 5V/cm, 1h Contents(bp) 100, 200, 300, 0,500,600,700,800,900,1000, 1200, 1500,2000,3000 Storage: 3 months at room temperature, 2 years at Tel:

12 DNA Ladders & Markers 1 kb ladder Cat: M µg, M1182-5x50µg 1% agarose 1kb ladder is ideal for determining the size of double-stranded DNA from 500 to 10000bp base pairs. The ladder consists of 10 linear double-stranded fragments. The 2000bp and 5000bp fragments are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 2000bp and 5000bp are ng. The 2000bp and 50 00bp ar e 10 0ng. This ladder is pr e-mixed wi th loading dy e an d is ready to us e. Concentration: 104ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 100ng/ l, Other bands: ng/ l Recommended Electrophoresis Condition 8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min Contents(bp) 500, 1000, 1500, 2000, 3000, 00, 5000, 6000, 8000, Storage: 3 months at room temperature, 2 years at DNA Laders & Markers 1kb ladder plus Cat. # M µg, M1192-5x50µg 1kb ladder plus is ideal for determining the size of double-stranded DNA from 100 to 10000bp base pairs. The ladder consists of 15 linear double-stranded fragments. The 500bp and 3000bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 500bp and 3000bp are ng. The 500bp and 3000bp fragment are 100ng. Thi s ladder is pre-mixed with loading dye and is ready to use. 1% agarose Concentration: 144ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 100ng/ l, Other bands: ng/ l Recommended Electrophoresis Condition 8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min Contents(bp) 100, 200, 300,0, 500,700,1000,1500,2000, 3000, 00, 5000,6000, 8000, Storage: 3 months at room temperature, 2 years at order@dongshengbio.com Fax:

13 Low ladder Cat: M µg, M1032-5x50µg 1 DNA Ladders & Markers 1% agarose 1% agarose 3% agarose 200bp ladder Low ladder is ideal for det ermining the size of doubl e-stranded DNA from 25bp to 700bp base pai rs. The ladder cons ists of 10 linear doubl e-stranded fragm ent s. The 100bp and 300bp fragm ent s are presen t at incr ease d intensi ty to allow easy ident ificat ion. All fragm ent s are preci sel y quant ified and mixed dur ing the produc tion. For 5 l loadi ng, all fragm ent s exc ept 100bp and 300bp are ng. The 100bp and 300bp are 100ng. This ladder is pre-mixed with loading dye and is ready to use. Concentration: 104ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 100ng/ l, Other bands: ng/ l Recommended Electrophoresis Condition 8cm 3% Agarose Gel, 0.5x TBE, 5V/cm, 1h Contents(bp) 25, 50,75, 100, 150,200, 300,0,500,700 Storage: 3 months at room temperature, 2 years at bp ladder is ideal for determining the size of double-stranded DNA from 200 to 00bp base pairs. The ladder consists of 12 linear double-stranded fragments. The 1000bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 1000bp is ng. The 1000bp fragment is 100ng. Thi s ladder is pre-mixed with loading dye and is ready to use. Concentration: 108ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 100ng/ l, Other bands: ng/ l Recommended Electrophoresis Condition 8cm 1% Agarose Gel, 1x TAE, 7V/cm, min Contents(bp) Cat. # M µg, M1152-5x50µg 200, 0, 600,800,1000,1200,10,1600,1800,2000,2200,00 Storage: 3 months at room temperature, 2 years at Tel:

14 DNA Ladders & Markers 500bp ladder Cat: M µg, M1252-5x50µg 0.7% agarose 1% agarose 500bp ladder is ideal for determining the size of double-stranded DNA from 500 to 5000bp base pairs. The ladder consists of 10 linear double-stranded fragments. The 2000bp fragme nt is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 loading, all fragments except 2000bp are ng. The 2000bp is 100ng. This ladder is pre-mixed with loading dye and is ready to use. Concentration: 92ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 100ng/ l, Other bands: ng/ l Recommended Electrophoresis Condition 8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min Contents(bp) Lambda DNA/Hind III 500,1000, 1500, 2000, 2500, 3000,3500,00, 4500, 5000, Storage: 3 months at room temperature, 2 years at -20. The Hind III digest of lambda DNA yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis. Lambda DNA/Hind III is pre-mixed with loading dye and is ready to use. Recommended Loading : 2-5 l/lane Cat. # M µg, M1202-5x50µg Recommended Electrophoresis Condition 8cm 0.7% Agarose Gel, 1x TAE, 7V/cm, 45min l 1 DNA Laders & Markers Contents(bp) 125, 564, 2027,2322,4361,6557,9461,23130 Storage: 3 months at room temperature, 2 years at order@dongshengbio.com Fax:

15 Marker 1 Cat: M µg, M1082-5x50µg 1 DNA Ladders & Markers 3% agarose Mar ker 1 is ideal for determining the size of double-stranded DNA from 100 to 600bp base pairs. The ladder consists of 6 linear double-stranded fragmen ts. The 0bp fragmen t is present at increased intensity to allow easy identification. All fragmen ts are precisely quantified and mixed during the production. For 5 l loading, all fragmen ts except 0bp are 50ng. The 0bp is 125ng. This ladder is pre-mixed with loading dye and is ready to use. Concentration: 76ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l Recommended Electrophoresis Condition 8cm 3% Agarose Gel, 0.5x TBE, 7V/cm, min Contents(bp) 100, 200, 300, 0, 500, 600 Storage: 3 months at room temperature, 2 years at -20. Marker 2 Cat. # M µg, M1092-5x50µg Marker 2 is ideal for determining the size of double-stranded DNA from 100 to 1200bp base pairs. The ladder consists of 6 linear double-stranded fragments. The 700bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 700bp are 50ng. The 700bp is 125ng. Thi s ladder is pre-mixed with loading dye and is ready to use. Concentration: 75ng/ l 1.7% agarose Recommended Loading : 2-5 l/lane Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l Recommended Electrophoresis Condition 8cm 1.7% Agarose Gel, 0.5x TBE, 5V/cm, 1h Contents(bp) 100, 300, 500, 600, 700, 900,1200 Storage: 3 months at room temperature, 2 years at Tel:

16 DNA Ladders & Markers Marker 3 Cat: M µg, M1122-5x50µg 1% agarose Marker 3 is ideal for determining the size of double-stranded DNA from 200 to 4500bp base pairs. The ladder consists of 7 linear double-stranded fragments. The 1200bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 1200bp are 75ng. The 1200bp is 150ng. This ladder is pre-mixed with loading dye and is ready to use. Concentration: 85ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 150ng/ l, Other bands: 75ng/ l Recommended Electrophoresis Condition 8cm 1% Agarose Gel, 1x TAE, 7V/cm, min Contents(bp) 200, 500, 800, 1200, 2000, 3000, 4500 Storage: 3 months at room temperature, 2 years at DNA Laders & Markers Marker 4 Cat. # M µg, M1232-5x50µg Marker 4 is ideal for det ermining the size of doubl e-stranded DNA from 500 to 15000bp base pai rs. The ladder cons ists of 6 linear doubl e-stranded fragm ent s. The 2500bp fragm ent is present at incr eased intensi ty to allow easy ident ificat ion. All fragm ent s are preci sel y quant ified and mixed dur ing the product ion. For 5 l loadi ng, all fragm ent s exc ept 2500bp are 50ng. The 2500bp is 125ng. Thi s ladder is pre-mixed with loading dye and is ready to use. Concentration: 75ng/ l Recommended Loading : 2-5 l/lane 1% agarose Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l Recommended Electrophoresis Condition 8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min Contents(bp) 500, 1200, 2500, 5000, 8000,15000 Storage: 3 months at room temperature, 2 years at order@dongshengbio.com Fax:

17 DS 2000 Cat: M µg, M1102-5x50µg 1 DNA Ladders & Markers 1.7% agarose DS 2000 is ideal for determining the size of double-stranded DNA from 100 to 2000bp base pairs. The ladder consists of 6 linear double-stranded fragmen ts. The 750bp fragmen t is present at increased intensity to allow easy identification. All fragmen ts are precisely quantified and mixed during the production. For 5 l loading, all fragmen ts except 750bp are 75ng. The 750bp is 150ng. This ladder is pre-mixed with loading dye and is ready to use. Concentration: 105ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 150ng/ l, Other bands: 75ng/ l Recommended Electrophoresis Condition 8cm 1.7% Agarose Gel, 0.5x TBE, 7V/cm, min Contents(bp) 100, 200, 500, 750, 1000, 2000 Storage: 3 months at room temperature, 2 years at -20. DS 5000 Cat: M µg, M1112-5x50µg DS 5000 is ideal for determining the size of double-stranded DNA from 100 to 5000bp base pairs. The ladder consists of 9 linear double-stranded fragments. The 1000bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 1000bp are 75ng. The 1500bp is 150ng. Thi s ladder is pre-mixed with loading dye and is ready to use. Concentration: 150ng/ l 1% agarose Recommended Loading : 2-5 l/lane Concentration: Typical bands: 150ng/ l, Other bands: 75ng/ l Recommended Electrophoresis Condition 8cm 1% Agarose Gel, 1x TAE, 7V/cm, min Contents(bp) 100, 250, 500, 750, 1000, 1500, 2000, 3000, 5000 Storage: 3 months at room temperature, 2 years at Tel:

18 DNA Ladders & Markers DS Cat: M µg, M1222-5x50µg 1% agarose DS is ideal for det ermining the size of doubl e-stranded DNA from 250 to 10000bp base pai rs. The ladder cons ists of 7 linear doubl e-stranded fragm ent s. The 2000bp fragm ent is presen t at incr ease d intensi ty to allow easy ident ificat ion. All fragm ent s are preci sel y quant ified and mixed dur ing the produc tion. For 5 l loadi ng, all fragm ent s exc ept 2000bp are 50ng. The 2000bp is 125ng. Thi s ladder is pre-mixed with loading dye and is ready to use. Concentration: 85ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l Recommended Electrophoresis Condition 8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min Contents(bp) 250, 500, 1000, 2000, 00,7000, Storage: 3 months at room temperature, 2 years at DNA Laders & Markers DS Cat: M µg, M1162-5x50µg DS is ideal for determining the size of double-stranded DNA from 250 to 10000bp base pairs. The ladder consists of 7 linear double-stranded fragments. The 2500bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 2500bp are 50ng. The 2500bp is 125ng. Thi s ladder is pre-mixed with loading dye and is ready to use. 1% agarose Concentration: 85ng/ l Recommended Loading : 2-5 l/lane Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l Recommended Electrophoresis Condition 8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min Contents(bp) 250, 1000, 2500, 5000, 7500, 10000, Storage: 3 months at room temperature, 2 years at order@dongshengbio.com Fax:

19 DNA Ladder/Marker Customized Service 1 DS is a collection of 30 highly-purified individual DNA fragments ranging from 10-15,000 bp. DNA Ladders & Markers DNA Fragnent Cat # Amount 10 bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M bp M8301 We offer exceptionally pure DNA fragments that are free of any truncated or degraded molecules. 0.1 Storage Buffer (TE Buffer) 10 mm Tris-HCl (ph 7.6) with 1 mm EDTA Quality Control Tested on an Agilent 2100 bioanalyzer and by appropriate gel electrophoresis. DNA concentration is determined by spectrophotometer. The absence of nucleases confirmed by appropriate tests.. Storage 2 years at -20 Custom DNA Ladders Service Bulk orders can be customized to fit your needs: Individual DNA fragments or mixtures. Custom ladders of any combination and concentration. Reference bands in a custom-made DNA ladder. Flexible formulations. Ready-to-use versions premixed with any loading dye. From microgram to gram quantities Tel:

20 PCR 1 PCR PCR PCR PCR PCR DNA Taq DNA HS Taq DNA Pfu DNA Taq Plus DNA Long Taq DNA FS Taq DNA PCR Mix PCR Mix HS Taq Mix Pfu Mix Plus Mix Long Taq Mix SYBR Green qpcr Mix Power SYBR Green PCR Master Mix Rox FS Mix FS Mix Direct for Blood FS Mix Direct for Tissue PCR Kit PCR Kit HS Kit Pfu Kit Long Taq Kit FS Kit PCR, qpcr & RT-PCR 5 PCR bp PCR bp PCR PCR PCR PCR dntps mm MgCl PCR Enhancer PCR Buffer PCR PCR

21 PCR Products PCR (polymerase chain reaction) is a technique that is central to molecular biology research. It is used in applications such as cloning, gene ex- pression analysis, genotyping, sequencing, and mutant genes. 2 PCR, qpcr & RT-PCR Dongsheng Biotech offers different series of products to help you achi- eve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long DNA amplification. We provi de four ser ies of PCR product s for different uses: PCR Polymerase Series PCR Mix Series PCR Kit Series qpcr Mix Series RT-PCR Ser ies 21 Tel:

22 PCR, qpcr & RT-PCR Propertie s of PCR enzymes Enzyme Feature Amplification size Fidelity Sensivity PCR Product ends Application Taq 5' 3' Activity '-dA General PCR/RT-PCR DNA Mark/DNA Sequence TA Clone HS Taq 5' 3'Activity '-dA General PCR/RT-PCR DNA Mark/DNA Sequence FS Taq Pfu Taq Plus LongTaq 5' 3' Activity Fast-3 times of Taq 5'? 3' Activity 5'-3' Exonclease activity 5'? 3' Activity 5'-3' Exonclease activity High fidelity-4 times of Taq 5' 3' Activity 5'-3' Exonclease activity High fidelity-4 times of Taq '-dA Blunt end '-dA/ Blunt end 3'-dA/ Blunt end TA Clone General PCR/RT-PCR DNA Mark/DNA Sequence TA Clone High fidelity PCR Blunt end vector cloning RT-PCR/cDNA clone and express/snp GC rich template Complicated template High repeat sequences GC rich template Long template Sequencing 2 PCR, qpcr & RT-PCR Fusion pfu 5'? 3' Activity 5'-3' Exonclease activity Blunt end High fidelity PCR Long fragment PCR More and more convenient More and more refind funtion Cored by genetic engineering technology Based by Taq DNA Polymerase order@dongshengbio.com Fax:

23 PCR, qpcr & RT-PCR PCR Products Product Name Cat # Taq DNA Polymerase P1011 P1012* P1013 P1014* 500U 500U 1,000U 1,000U Robust Taq DNA Polymerase for routine use incl.10x PCR Buffer, 6xloading buffer, Mg2+ plus or free. Supply with 5U/ µl and 2.5U/ µl packages. 2 HS Taq DNA Polymerase P1015 P1081 P1082 P1083 P ,000U 250U 500U 1,000U 18,000U High specificity, incl. 10x HS PCR buffer, 6x loading buffer, Mg2+ plus or free. Supply with 5U/ µl and 2.5U/ µl two packages. PCR, qpcr & RT-PR Pfu DNA Polymerase Taq Plus DNA Polymerase Long Taq DNA Polymerase P1021 P1022* P1023 P1024* P1031 P1032* P1033 P1034* P1061 P1062* P1063 P1064* 250U 250U 1,000U 1,000U 250U 250U 1,000U 1,000U 250U 250U 1,000U 1,000U High fidelity, incl.10x pfu buffer, 6xloading buffer, Mg2+ plus or free. Supply with 5U/ µl and 2.5U/ µl two packages. Mix with Taq Polymerase and Pfu Polymerase, long amplification and high fidelity. Incl. 10xTaq Plus buffer, Mg2+ plus or free. Supply with 5U/ µl and 2.5U/ µl two packages. For long PCR, incl. 10xLong Taq buffer, 6xloading buffer, Mg2+ plus or free. Supply with 5U/ µl and 2.5U /µl two packages. FS Taq DNA Polymerase P1071 P1072* P U 250U 1,000U Fast, incl. 10xFS PCR buffer, 6xloading buffer, Mg2+ plus or free. Supply with 5U/ µl and 2.5U/ µl two packages. P1074* 1,000U Taq Mix (2x) P2011 Pre mix with Taq DNA Polymerase, dntps, P2011 Mg2+ and Reaction buffer at optional concentrations HS Mix (2x) P2081 P2082 Pre mix with HS Taq DNA Polymerase, dntps, Mg2+ and Reaction buffer at optional concentrations Pfu Mix (2x) P2021 P2022. Pre mix with Pfu DNA Polymerase, dntps, Mg2+ and Reaction buffer at optional concentrations. Plus Mix (2x) P2031 P2031 Pre mix with Taq Plus DNA Polymerase, dntps, Mg2+ and Reaction buffer at optional concentrations. Long Mix (2x) FS Mix (2x) FS Taq Mix Direct for Blood (2x) FS Taq Mix Direct for Tissue (2x) P2061 P2062 P3011 P3012 P3021 P3022 P3031 P3032 5x1m Pre mix with Long Taq DNA Polymerase, dntps, Mg2+ and Reaction buffer at optional concentrations. Pre mix with FS Taq DNA Polymerase, dntps, Mg2+ and Reaction buffer at optional concentrations. Pre mix with FS Taq DNA Polymerase, dntps, Mg2+ and Reaction buffer at optional concentrations. Contains FS Taq Mix T(2x) and sample preparation solution (Extraction solution and Neutralization solution 23 Tel:

24 PCR, qpcr & RT-PCR Product Name Cat # PCR Kit HS Kit Pfu Kit Long Taq Kit FS Kit dntp Mix (2.5mM) dntp Mix (10mM) datp (100mM) dttp (100mM) dctp (100mM) dgtp (100mM) 10xPCR buffer MgCl (25mM) 2 Nuclease-free Water PCR Enhancer PCR Sample Preparation Solution qpcr Products P3012 P3082 P3022 P3062 P3072 P9011 P9012 P9013 P9071 P9081 P9091 P9101 P5011 P5011a P5011b P9031 P9021 P9022 P9023 P9041 P9051 P order@dongshengbio.com Fax: ml 0.5ml 0.5ml 0.5ml 0.5ml 4x1.25ml 4x1.25ml 6x 4x1.25ml 100ml 500ml 500ul 50 preps 250 preps Contains 250U Taq DNA Polymerase and CR Reaction Mix (2x) Contains 250U HS Taq DNA Polymerase and HS Reaction Mix (2x) Contains 250U Pfu DNA Polymerase and Pfu Reaction Mix (2x) Contains 250U FS Taq DNA Polymerase and FS Reaction Mix (2x) Contains 250U Long Taq DNA Polymerase and Long Reaction Mix (2x) With 15mM Mg2+ Without 15mM Mg2+ With Mg2+ concentration set To prepare blood or tissue sample for fast PCR Product Name Cat # SYBR Green qpcr Mix SYBR Green qpcr Mix (Rox + ) RT-PCR Products Product Name Cat # M-MLV (recombinant) RT-PCR RNasin (RNase inhibitor) Oligo d(t) Primer 15 Random Primer (6mer) DEPC-treated Water RNA Stabilization Solution P2091 P2092 P2091a P2092a P2091b P2092b R1041 R1042 R1011 R1012 R2011 R2021 R2031 R2041 R2042 R2071 R U 10000U 20 rxns 100 rxns 1000U 20µl 20µl 30ml 100ml 20ml 100ml Mix reaction (2x) for real-time PCR With low Rox+ With high Rox PCR, qpcr & RT-PCRs

25 Taq DNA Polymerase -routine Taq 2 Code P U P1012* 500U P U P1014* 1000U P ,000U * means supplied with dntps. Taq DNA Polymerase is a themostable recombinant DNA Polymerase derived from Thermos aquaticus. It is a robust Taq for routine PCR use. Its molecular weight is 94 kda. Taq DNA Polymerase can amplify DNA target up to 5kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75 ). It has 5'? 3' exonuclease activity but lacks 3'? 5 exonuclease activity that results in a 3'-dA overhangs PCR product. Contents: Taq DNA Polymerase(5U/ l), 10xPCR Buffer, 6xLoading buffer, dntps*(p1012, P1014) PCR, qpcr & RT-PCR Code P1081 P1082 P1083 P U 500U 1000U 18,000U HS Taq DNA Polymerase -high specificity HS Taq DNA Polymer ase is a thermos table recombi nant DNA Polymer ase derived from Thermos aquaticus. It contents the different reaction buffer that is designed to provide highly specific ampl ification of nucleic acid templ ates by PCR. The elongation velocity is 0.9~1.2kb/min (70~75 ).It is heat-stable and will synthesize DNA at elevated tempe ratures from single-stranded templ ates in the presence of a primer. Contents: HS Taq DNA Polymerase(5U/ l), 10xHS PCR Buffer, 6xLoading buffer Pfu DNA Polymerase -high fidelity Code P U P1022* 250U P U P1024* 1000U * means supplied with dntps. Pfu DNA Polymerase has superior thermostability and proofreading properties compared to other thermostable polymerase. It can amplify DNA target up to 2kb (simple template). Its fidelity can reach 10 time of Taq DNA Polymerase. The elongation velocity is 0.2~0.4kb/min (70~75 ). It results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors.pru DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis. Contents: Pfu DNA Polymerase(2.5U/ l), 10xPfu Buffer, 6xLoading buffer, dntps* (P1022, P1024) 25 Tel:

26 PCR, qpcr & RT-PCR Taq Plus DNA Polymerase -high fidelity, high efficiency Code P1031 P1032* P1033 P1034* 250U 250U 1000U 1000U Taq Plus DNA Polymerase is a mixture of Taq and Pfu polymerase, blends the processivity of taq with the high fidelity of pfu. The two enzymes act synergistically during PCR to generate more accurate, longer and greater yield PCR products. It can amplify DNA target up to 20kb (simple template). The error rate -5 is 7.5x10 per nucleotide per cycle. * means supplied with dntps. Contents: Taq Plus DNA Polymerase(2.5U/ l), 10xPCR Buffer, 6xLoading buffer, dntps*(p1032, P1034) 2 Code P1061 P1062* P1063 P U 250U 1000U 1000U Long Taq DNA Polymerase Contents: Long Taq DNA Polymerase(5U/ l), 10x Long PCR Buffer I, 10x Long PCR Buffer II PCR Enhancer, 6xLoading buffer -for complex templates Long Taq DNA Polyme rase, a comb ination of Taq and Pfu, is a special formu lation desigen for amplifying large fragment. This specially formulated Long Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying comp lex temp lates, such as GC-rich temp lates. It will result in 3 -da overhangs PCR products, which can be used In TA clone. PCR, qpcr & RT-PCR FS Taq DNA Polymerase -fast Taq Code P U P1072* 250U P U P1074* 1000U * means supplied with dntps. FS Taq DNA Polymerase, developed by Dongsheng Biotech, is the latst generation Taq-based DNA Polymerase. It processes high amplification efficiency as Taq polymerase does, and fast elongation ability as KOD polymerase does,can be used as a variety of PCR. The elongation rate of FS Taq is 2 times higher than regular Taq. It will save the amplification time by half. Contents: FS Taq DNA Polymerase(5U/ l), 10xFS PCR Buffer, 6xLoading buffer order@dongshengbio.com Fax:

27 Taq Mix (2x) -routine PCR 2 Code P2011 P2012 Taq Mix (2x) is a premixed, ready-to-use solution containing Taq DNA Polym- 2+ erase, dntps, Mg and Reaction Buffer at optional concentrations for efficient PCR amplification. To prepare the final PCR, only primers and template DNA are added. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The mix retains all features of Taq DNA Polymerase. Contents: 2xTaq Mix, Nuclease-free Water PCR, qpcr & RT-PCR Code P2081 P2082 HS Mix (2x) -high specificity HS Mix (2x) is a premixed, ready-to-use solution containing HS HS Taq DNA 2+ Polymerase, dntps, Mg and Reaction Buffer at optional concentrations for efficient PCR amplification. To prepare the final PCR, only primers and template DNA are added. Mix provides increased sensitivity, specificity, and yield, while allowing assembly of reactions at RT. This mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It is convenient and also save time. Contents: 2xHS Mix, Nuclease-free Water Pfu Mix (2x) -high fidelity Code P2021 P2022 Pfu Mix (2x) is a premixed, ready-to-use solution containing Pfu Taq DNA 2+ Polymerase, dntps, Mg and Reaction Buffer at optional concentrations for efficient PCR amplification. To prepare the final PCR, only primers and template DNA are added. Pfu Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding intersifier and optimizer. Pfu Taq is superior for technique that requires high fidelity DNA synthesis. Contents: 2x Pfu Mix, Nuclease-free Water 27 Tel:

28 PCR, qpcr & RT-PCR Plus Mix (2x) -high fidelity, high efficiency Code P2031 P2032 Plus Mix (2x) is a premixed, ready-to-use solution containing Taq Plus DNA 2+ Polymerase, dntps, Mg and Reaction Buffer at optional concentrations for efficient PCR amplification. To prepare the final PCR, only primers and template DNA are added. Plus Mis contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It contains both Contents: 2xPlus Mix, Nuclease-free Water 2 Long Taq Mix (2x) Code P2061 P2062 -for complex templates Long Taq Mix (2x) is a premixed, ready-to-use solution containing Long Taq DNA 2+ Polymerase, dntps, Mg and Reaction Buffer at optional concentrations for efficient PCR amplification. To prepare the final PCR, only primers and template DNA are added. Plus Mis contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes higher sensitivity by adding enhancer. It is good for complex templates, such as GC-rich templates. PCR, qpcr & RT-PCR Contents: 2xLong Taq Mix, Nuclease-free Water FS Mix (2x) -fast PCR Code P2071 P2072 FS risk of pipetting errors, miscalculation and contamination. The elongation rate of. FS Mix (2x) is a premixed, ready-to-use solution containing FS Taq DNA 2+ Polymerase, dntps, Mg and Reaction Buffer at optional concentrations for efficient PCR amplification. To prepare the final PCR, only primers and template DNA are added.this mix contributes to highly reproducible PCR by reducing the Taq is 2 times higher than regular Taq Contents: 2x FS Mix, Nuclease-free Water order@dongshengbio.com Fax:

29 FS Mix Direct for Blood (2x) 2 Code P2071a P2072a whole or diluted blood 2x FS Mix B FS Mix Direct for Blood (2x) is a premixed, ready-to-use solution containing FS Taq DNA Polymerase, dntps, and all other PCR components, except DNA template and primers. This mix is specific for whole blood amplification. It contribute to fast, specific, sensitive and reproducible PCR by reducing the fisk of contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. This product cut off the process of complicated and waste time DNA extraction. PCR, qpcr & RT-PCR primers water Contents: 2x FS Mix B, Nuclease-free Water FS Mix Direct for Tissue (2x) Code P2071b P2072b Sample preparation prepared sample FS Mix Direct for Tissue (2x) is a premixed, ready-to-use solution containing FS Taq DNA Polymerase, dntps, and all other PCR components, except DNA template and primers. This mix is specific for tissue direct amplification. It contribute to fast, specific, sensitive and reproducible PCR by reducing the fisk of contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. This product cut off the process of complicated and waste time DNA extraction. 2x FS Mix T Contents: 2x FS Nuclease-free Water Mix T, Extraction Solution, Neutralization Solution primers water 29 Tel:

30 PCR, qpcr & RT-PCR PCR Kit -routine PCR Code P3012 The PCR Kit contains a recombinant, high purified Taq DNA Polymerase, and a premixed, ready-to-use 2xPCR Reaction Mix to perform 200 PCR reactions. Code P xPCR Reaction Mix is a premixed solution containing dntps, Mg and PCR buffer at optional concentrations for efficient PCR amplification. PCR amplification sensitivity is controllable because the amount of Taq DNA Polymeras is flexible. Contents: 2xPCR Reaction Mix, Taq DNA Polymerase(2.5U/ l) HS Kit -high specificity HS Kit contains a HS Taq DNA Polymerase, and a premixed, ready-to-use 2x HS Reaction Mix to perform 200 PCR reaction. 2x HS HS 2+ Reaction Mix is a premixed solution containing dntps, Mg and buffer at optional concentrations for efficient PCR amplification. PCR amplification sensitivity is controllable because the amount of Polymerase is flexible. HS Taq DNA 2 PCR, qpcr & RT-PCR Contents: 2xHS Reaction Mix, HS Taq DNA Polymerase (2.5U/ l) Pfu Kit -high fidelity Code P3022 Pfu Kit contains a Pfu DNA Polymerase, and a premixed, ready-to-use 2xPfu Reaction Mix to perform 200 PCR reactions. 2+ 2xPfu Reaction Mix is a premixed solution containing dntps, Mg and Pfu buffer at optional concentrations for efficient PCR amplification. PCR amplification sensitivity is controllable because the amount of Pfu DNA Polymeras is flexible. Contents: 2xPfu Reaction Mix, Pfu DNA Polymerase(2.5U/ l) order@dongshengbio.com Fax:

31 Long Taq Kit -for complex templates Code P3062 Long Taq Kit contains a Long Taq DNA Polymerase, PCR Enhancer, and a premixed, ready-to-use 2xPCR Reaction Mix to perform 200 PCR reactions. 2 PCR, qpcr & RT-PCR Code P xLong Reaction Mix is a premixed solution containing dntps, Mg and Long PCR buffer at optional concentrations for efficient PCR amplification. PCR amplification sensitivity is controllable because the amount of Lont Taq DNA Polymeras. is flexible Contents: 2xLong Reaction Mix, PCR Enhancer, Taq DNA Polymerase(2.5U/ l) FS Kit -fast PCR FS Kit contains a FS Taq DNA Polymerase, and a premixed, ready-to-use 2x FS Reaction Mix to perform 200 PCR reaction. 2x FS FS 2+ Reaction Mix is a premixed solution containing dntps, Mg and buffer at optional concentrations for efficient PCR amplification. PCR amplification sensitivity is controllable because the amount of Polymerase is flexible. FS Taq DNA Contents: 2xFS Reaction Mix, FS Taq DNA Polymerase (2.5U/ l) dntps Product Name Code dntp Mix (2.5mM) dntp Mix (10mM) dntp Mix (10mM) dntp Set (100mM) datp (100mM) dttp (100mM) dctp (100mM) dgtp (100mM) dntps (10mM, RNase-free) P9011 P9012 P9013 P9014 P9071 P9081 P9091 P9101 R ml 4x0.25ml 0.5ml 0.5ml 0.5ml 0.5ml 0.5ml dntp Mix is a aqueous solutions at PH 7.0 containing datp, dttp, dctp, dgtp each at a final concentration of 2.5mM (or 10mM). dntps (10mM, RNase-free) is ideal for RT- PCR reaction Tel:

32 PCR, qpcr & RT-PCR PCR Buffer Product Name Code 2+ 10xPCR Buffer (with 15mM Mg ) P xPCR Buffer (without Mg ) 10xPCR Buffer 2+ (with Mg concentration set) 25mM MgCl2 P5011a P5011b P9031 4x1.25ml 4x1.25ml 6x1.25ml 4x1.25ml 10xPCR Buffer is recommended for all PCR applications with Taq DNA Polymerase (both recombinant or native) 2 Nuclease-free Water Product Name Nuclease-free Water PCR Enhancer Product Name PCR Enhancer Code P9021 P9022 P9023 Code P ml 500ml 0.5ml Nuclease-free Water is deionized and 0.22 m membrane-filtered. It is suitable for all molecular biology applications. PCR Enhancer is used in conjunction with DNA polymerase to optimized PCR of pro- PCR, qpcr & RT-PCR blemetic and/or GC-rich templates. The system offers higher specificity, broader magnesium concentration, broader annealing temperature and improved thermostable DNA polymerase. PCR Sample Preparation Solution Product Name PCR Sample Preparation Solution Code P pres P preps Storage: 2 years at room temperature Contents: Extraction Solution Neutralization Solution PCR Samp le Preparation Solution is widely used for sample preparation before PCR reaction. It can be used for samp les like tissue, hair, buccal swab, culture cells, serum, DNA virus. order@dongshengbio.com Fax:

33 Real Time qpcr Master Mix 2 Keep Features: High Specificity eliminate non specific and formation of primer dimers High Sensitivity detects low copy number targets Universal suitable for most real time thermal cyclers Product SYBR Green qpcr Mix SYBR Green qpcr Mix (High Rox+) SYBR Green qpcr Mix (Low Rox+) Probe qpcr Mix Probe qpcr Mix (High Rox+) Probe qpcr Mix (Low Rox+) PCR, qpcr & RT-PCR Plasmid DNA 10ng, 1ng, 0.1ng, 0.01ng, 0.001, ng ul in BIO RAD CFX96 Dongsheng Company A Company A Dongsheng Plasmid DNA 1ng, 0.1ng, 0.01ng, 0.001ng/ul in BIO-RAD CFX96 Instrument guide table Machine Rox Reference Dye 33 Tel:

34 PCR, qpcr & RT-PCR SYBR Green qpcr Mix -realtime PCR Code P2091 P2092 SYBR Green qpcr Mix (2x) is designed for high-performance, high-throughput, real-time PCR. The kit contains a Hot Start Taq DNA Polymerase engineered a process of molecular evolution. This results in a unique enzyme, specifically designed for qpcr using SYBR Green I dye chemistry. Recommended PCR Reaction ( 20µl reaction system) SYBR Green qpcr Mix (2x) is a convenient premix of the components (except primers, template, and water) necessary to perform real-time polymerase chain reaction (PCR) using SYBR Green I dye with enhanced sensitivity and specificity. The SYBR Green dye binds to double-strand (ds) DNA, thus providing a fluoresent signal that reflects the amount of dsdna product generated during PCR. Contents: 2xSYBR Green qpcr Mix, Nuclease-free Water Storage: Protect from light, 2 years at -20. Components Volume Final concentration 2xSYBR Green qpcr Mix 10 µl 1X Forward primer (10µM) 0.4 µl 0.2 µm Reverse primer (10 µm) 0.4 µl 0.2 µm Template DNA variable 100ng Nuclease-free, Water to 20 µl - 2 PCR, qpcr & RT-PCR Initial Denaturation 94 3 min Recommend PCR Cycling System Denature Anneal Extend* sec 15 sec 20 sec cycles Melting Curve Analysis Dongsheng cat #P9021/P9022 work better for 3 step cycling protocol. We don t recommend to use 2 step cycling protocol with this product. Amplification Melt Curve Above are with Dongsheng s SYBR Green qpcr Mix in STRATAGENE Mx3005. Sample: HBV Serum, Objective band: 210bp. Ampification condition: 95C 20s in the first cycle, 95C 10s, 55C 20s and 72C 30s in the less cycles. order@dongshengbio.com Fax:

35 + SYBR Green qpcr Mix (Rox ) -high accurate real-time PCR 2 PCR, qpcr & RT-PCR Code P2091a P2092a P2091b P2092b Recommended PCR Reaction ( 20µl reaction system) SYBR Green qpcr Mix + (Rox ) (2x) is designed for high-performance, highthroughput, real-time PCR. The kit contains a Hot Start Taq DNA Polymerase engineered a process of molecular evolution. This results in a unique enzyme, specifically designed for qpcr using SYBR Green I dye chemistry. It also contains rox reference dye, which is ideal for using in amplification and detection of DNA in qpcr on real-time instruments (e.g. ABI) that supports normalization with rox reference dye. Contents: + #P2091a/P2092a: 2xSYBR Green qpcr Mix (Low Rox ), Nuclease-free Water #P2091b/P2092b: 2xSYBR Green + qpcr Mix (High Rox ), Nuclease-free Water Storage: Protect from light, 2 years at -20. Components + 2xSYBR Green qpcr Mix (Rox ) Forward primer (10µM) Reverse primer (10 µm) Template DNA Nuclease-free, Water Volume 10 µl 0.4 µl 0.4 µl variable to 20 µl Final concentration 1X 0.2 µm 0.2 µm 100ng - Initial Denaturation 94 3 min Recommend PCR Cycling System Denature Anneal Extend* sec 15 sec 20 sec cycles Melting Curve Analysis Dongsheng cat #P9021a/P9022a/P9021b/P9022b work better for 3 step cycling protocol. We don t recommend to use 2 step cycling protocol with this product. Amplification Melt Curve Above are with Dongsheng s SYBR Green qpcr Mix (High Rox in STRATAGENE Mx Tel:

36 Reverse Transcriptase PCR, qpcr & RT-PCR M-MLV (recombinant) Cat.# R U, R U Contents M-MLV (200U/ µl) 5xfirst-strand buffer (5x) R µl 100 µl R µl 200 µl R1041can be used for 25 reactions, R1042 can be used for 50 reactions (20 µl PCR system). Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand. This enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cdna up to 5kb. 2 RT-PCR Kit Contents M-MLV (200U/ µl) RNasin (U/µl) Oligo d(t) 15 (50µM) Random primer (50µM) 5xfirst-strand buffer (5x) RNase-free ddh2o dntps (10mM each) R µl 12µl 20µl 20µl 80µl 50µl R µl 60µl 100µl 100µl 0µl 250µl Cat.# R rxns, R rxns RT-PCR Kit is optimized to synthesize first-strand cdna from purified poly (A)+ or total RNA. The kit Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthsize cdna at a temperature range of 42-55, providing increased specificity, higher yields of cdna, and more full-length product than other reverse transcriptased. PCR, qpcr & RT-PCR Rnasin (RNase inhibitor) Cat.# R U Contents RNasin (U/µl) R µl Rnase is a ribonuclease inhibitor purified form human placenta. RNase recuces the activity of RNase A-type enzymes in a variety of organisms. It is free of endodeoxyribonucleases, exodeoxyribonucleases, ribonucleases and phosphatases. order@dongshengbio.com Fax: