BioLane HTI 16V Automated In Situ Hybridization and Immunohistochemistry
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1 Automated In Situ Hybridization and Immunohistochemistry
2 Overview History Applications Hardware Accessories Software for Western blot processing
3 History BioLane HTI Developed in 2001 by H&H in cooperation with JOBO as an automated system for (Western) blot staining In situ application officially launched in 2003 with 48-well plates; especially popular in zebra fish community Introduction of individual baskets 2004 Development of the slide tub 2005 June 2008, Intavis and H&H instrument joined together In 2008, development of the new, an advanced versionof theoldbiolanehti Totally installed units about 170, exclusively Western (of these for prionics test ~35)
4 Applications In situ hybridization Immunohistochemistry Western blot processing
5 Hardware Two systems in one
6 Hardware Two systems in one Two independent incubation units heating/cooling shaking System 1 System 2
7 Hardware Two systems in one System 1 System 2 Two independent incubation units heating/cooling shaking Two independent peristaltic pumps for buffer delivery and recovery pump 1 pump 2
8 Hardware Two systems in one air pump 1 air pump 2 pump 1 pump 2 System 1 System 2 Two independent incubation units heating/cooling shaking Two independent peristaltic pumps for buffer delivery and recovery Two independent air pumps for antibody delivery and recovery
9 Hardware Two systems in one air pump 1 air pump 2 cooled positions pump 1 pump 2 System 1 System 2 Two independent incubation units heating/cooling shaking Two independent peristaltic pumps for buffer delivery and recovery Two independent air pumps for antibody delivery and recovery Cooled positions 2x2 positions for 50 ml Falcons or 125ml vials individual tube paths
10 Hardware 16 way valve 16 way valve One 16 way valve for buffer selection 2x 14 positions for buffers 2x 2 positions for waste independent connections for both systems
11 Hardware PDA operated Operated by touch screen PDA running the instrument easy by PDA LAN connection for online monitoring programming via external PC or PC in the network via remote control internal PDA
12 Hardware Configurations Tray plus accessories for application + Medium baskets for ISH/IHC up to 40 samples/unit + Large baskets for ISH/IHC of larger specimens up to 24 samples/unit + plates for ISH/IHC screen up to 48 samples/unit + Slides for washing ISH/IHC of sections up to 25/unit Only trays for Western Blot up to 4 blots/unit, depending on size + 2D incubation unit and trays for 2D-Western, up to two/unit
13 Accessories trays 4 different tray sizes Extra small 12-16ml Small 25-30ml Medium 50-60ml Double medium ml
14 Accessories incubation baskets Extra small: Up to 8 medium Small: Up to 20 medium Medium: Up to 40 medium Extra small: Up to 6 large Small: Up to 12 large Medium: Up to 24 large
15 Accessories medium baskets hybridization Hybridization in vials
16 Accessories large baskets hybridization Hybridization in plate
17 Accessories plate and plate incubation Only for medium tray: 48 medium in plate
18 Accessories slide incubation Only washing Up to 25 slides/ incubation unit
19 Software Structure Self-test Run mode Switch to Edit mode Open method Information Start run/pause run Print : method/log file Switch off system Edit mode Switch to Run mode Open method Information User account administration Config Service
20 Software Self-test
21 Software The Run mode 1. Status line 2. TCP/IP button to connect to a network 3. System information 4. Method navigation arrows 5. Method step numbers 6. Red system (system 1) 7. Blue system (system 2) 8. Combi step 9. Icon bar
22 Software The Edit mode 1. Status line 2. Small icon bar to edit programs 3. Program information 4. Method navigation arrows 5. Method step numbers 6. Inpump step 7. Incubation step 8. Outpump step 9. Icon bar
23 Software Programming Program name Tray category Base tray type
24 Software Steps Step name Inpump step Incubation step Combistep Outpump step Progress bar
25 Software Information Volume information Runtime information
26 Software Online monitoring and remote control System can be directly connected to a PC by a crossed cable or to a LAN network using TCP/IP Via remote control, you can directly operate the system or monitor progress online Reminders can be send by when program finished Protocols can be sent by
27 Applications In situ hybridization Immunohistochemistry Western blot processing
28 Applications Western blot processing Separation of a protein mixture by SDS-gelelectrophoresis Transfer to and immobilization on membrane Processing for detection typically 3x washing primary antibody 3x washing secundary antibody 3x washing Detection Western Blotting Western Blot Processing Can be automated by the
29 Western Blot Processing Why Improves results Independent tube paths for buffers, primary and secondary AB Antibody binding easy to optimize due to temperature control Luffing, no round shaking for max liquid exchange Fluorescent antibodies can be used due to non-transparent lid Increases reproducibility: Time, volume, temperature reproducibly the same, independent from environmental conditions Standardization even for untrained personal Automated GLP reports possible Better comparability due to standardized protocols. Better initial data better quantification results (especially 2D gels) Saves time System can work o/n as well Higher temperature allows shortening of incubation times Can handle and recover antibodies Uninterrupted cold chain Vial does not have to be changed Higher consumption, but can be recovered and reused
30 Western Blot Processing Competition Competitors LI-COR Blot Washer Stovall Blot Washer Freedom Rocker and others Simple tumblers with pumping system Compared to No significant improvement of results Usually no independent tube path for antibody No easy optimization of binding conditions Limited increase in reproducibility Usually no heating/cooling of incubation units Time saving limited Usually no automated application of antibody Unsufficent antibody handling No uninterupted cold chain or recovery Li-COR Blot washer BUT: cheap!
31 Western Blot Processing Potential customers Prionics (BSE testing) Clinical applications, e.g. high safety labs (S3+/L3+) 2D-Western Industrial (GLP-reports) Western blot
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A helping hand for your research Product Manual Configuration-specific Monoclonal Antibody Based Arf6 Activation Assay Kit Catalog Number: 82401 20 assays NewEast Biosciences 1 Table of Content Product