catalog 2011 Reagents and Software for Flow Cytometry

Transcription

1 catalog 2011 Reagents and Software for Flow Cytometry

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3 catalog 2011 Introduction... 4 Antibodies (Ab) against Human Antigens New Fluorochrome-Conjugated Antibodies... 6 Index Single color antibodies against Human Antigens... 9 Double Color Combinations Triple Color Combinations Other combinations of antibodies Isotype Controls Reagents Erythrocyte Lysing Solution Quicklysis TM Reagents for Lymphocyte Subset Studies Lymphogram Lymphoclonal TM Reagents for DNA Content and Cell Cycle Studies Cycloscope TM Reagents for Monitoring of Monoclonal Gammopathies Clonalpath TM Clonalpath TM - Plus Reagents for Dendritic Cell Studies Absolute Counting Systems Perfect Count TM LeucoFinder TM Screening Kit for Paroxismal Nocturnal Hemoglobinuria (PNH) Software Flow Cytometry Data Analysis Software INFINICYT TM... 28

4 Introduction 4 Presentation Cytognos is a biotechnology company committed to advancing Flow Cytometry through the design and development of new reagents, software and techniques to be translated into innovative solutions for diagnostic and life science. Cytognos is certified to the quality management standards ISO 9001:2008 and ISO 13485:2003 for the design, development, production and sales of antibodies and kits for flow cytometry In Vitro Diagnostics and Research. IVD products, included in this catalog, have the CE mark in accordance with 98/79/EC Directive by the European Parliament. The CE mark guarantees the highest level of safety and healthy for patients, users and third parties. New products are developed in close cooperation with international universities and innovative companies in order to rapidly expand our experience to your laboratory. Our staff, devoted to the development of new technologies to satisfy customer needs, is pleased to offer our technical support. Cytognos has direct sales and a worldwide network of distributors. Please visit our website for more information. Regulatory Status The CE mark has its origins from the French Conformité Européenne or European Conformity and it is an European brand for certain groups of services or industrial products. It was established by the European Community and it is the testimony by the manufacturer that its product meets the minimum legal and technical requirements on safety matters from the Member States of the European Union. On basis to this legal framework, products offered in this catalog are labeled as: Medical Devices for In Vitro Diagnostic use (marked as CE / IVD For In Vitro Diagnostic Use ) are reagents to be used in vitro for the examination of specimens derived from the human body, for the purpose of providing information concerning a physiological or pathological state. CE/IVD marked products offer to patients, users and others parties, the highest levels of safety and operational qualities assigned by the manufacturer in conformity to Directive 98/79/EC of the European Union. Research Use Only (RUO) are reagents in the laboratory testing phase of development that does not have a recognized clinical utility. These products should not be used for clinical diagnosis, prognosis or therapeutic determinations.

5 Introduction 5 Reagents for use in Flow Cytometry Cytognos provides a variety of cellular analysis products to satisfy Flow Cytometry laboratory needs. We offer complete solutions that combine reagents for instrument standardization, fluorochrome-conjugated antibodies, isotype controls, solutions for sample preparation, and analysis software that will improve the performance of your studies. All our available fluorochrome-conjugated antibodies against human antigens are usually offered as single color but two or more colors combinations can be produced to meet your needs. Abbreviation Name Excitation Laser Line (nm) Excit-Max (nm) Emis-Max (nm) Recommend Band Pass Filter (nm) CF TM Blue CF405 TM Blue OC 515 Orange Cytognos 515 FITC PE PerCP-Cy5.5 PECy5 PECy7 APC APC-Cy7 Fluorescein Isothiocyanate R-Phycoerythrin Peridin chlorophyll protein -Cyanine 5.5 Tandem R-Phycoerythrin -Cyanine 5 Tandem R-Phycoerythrin -Cyanine 7 Allophycocyanine Tandem Allophycocyanine -Cyanine 7 Violet Laser / 405 nm Violet Laser / 405 nm Blue Laser / 488 nm Blue Laser / 488, 532, 561 nm Blue Laser / 488, 532 nm Blue Laser / 488, 532, 561 nm Red Laser / 488, 532, 561 nm Red Laser / 595, 633, 635, 640, 647 nm Red Laser / 595, 633, 635, 640, 647 nm / / /30 496, / LP 496, , / / /60

6 6 Antibodies (Ab) against Human Antigens New Fluorochrome-Conjugated Antibodies The violet laser is becoming a standard component of flow cytometers and is often used for common phenotyping markers in multicolor staining panels. Cytognos SL offers two new fluorochromes for use with the violet laser, which provide maximum resolution of antigenic determinants when used in a multicolor panel. Their narrow emission peaks result in little spectral overlap, which require minimal intra-laser compensation. CF TM Blue is a dye with similar spectral properties to Pacific Blue which emits at 450nm. It is characterized by: Narrow and well separated emission profiles. The relative stain indexes, which reflect the effective brightness of the antibody to differentiate between the positive and negative population, are optimal. As no excitation is detected in a 488nm laser, compensation requirements are minimal. Compatible with the standard filter sets of cytometers equipped with violet lasers. Spectral overlay comparison of CF TM Blue and Pacific Blue ABSORPTION EMISSION Other fluorochromes with similar spectrum. WAVELENGTH (nm) Fluorochrome Excitation Laser (nm) Peak Emission(nm) CF TM Blue 405, Pacific Blue 405, BD Horizon V , efluor ,

7 Antibodies (Ab) 7 Cytognos antibodies conjugated with this fluorochrome. Description Clone Format Reference Use CD3-CF TM Blue GA60 0,5 ml / 100 test CYT-3CFB RUO CD4-CF TM Blue H2P/6 0,5 ml / 100 test CYT-4CFB RUO CD20-CF TM Blue LT20 0,5 ml / 100 test CYT-20CFB RUO CD45-CF TM Blue GA90 0,5 ml / 100 test Coming soon RUO Kappa-CF TM Blue Polyclonal 0,5 ml / 100 test Coming soon RUO Lambda-CF TM Blue Polyclonal 0,5 ml / 100 test Coming soon RUO Peripheral blood sample stained with CYT-4CFB. Orange Cytognos 515 (OC515) is a dye with similar spectral properties to Pacific Orange which emits at 515nm. It is characterized by: Narrow and well separated emission profiles. The relative stain indexes, which reflect the effective brightness of the antibody to differentiate between the positive and negative population, are optimal. As no excitation is detected in a 488nm laser, compensation requirements are minimal. Minimal intra-laser compensation requirements. Absortion and emission Spectra of Orange Cytognos 515. Compatible with the standard filter sets of cytometers equipped with violet lasers.

8 8 Antibodies (Ab) Other fluorochromes with similar spectrum. Fluorochrome Excitation Laser (nm) Peak Emission(nm) Orange Cytognos , Pacific Orange 405, BD Horizon V , AmCyan 405, Krome Orange TM 405, efluor 605NC 405, efluor 650NC 405, Cytognos antibodies conjugated with this fluorochrome. Description Clone Format Reference Use CD45-OC515 GA90 0,5 ml / 100 test CYT-45OC RUO CD3-OC515 GA60 0,5 ml / 100 test Coming Soon RUO CD4-OC515 H2P/6 0,5 ml / 100 test Coming Soon RUO CD138-OC515 B-A38 0,5 ml / 100 test Coming Soon RUO Lambda-OC515 Polyclonal 0,5 ml / 100 test Coming Soon RUO Kappa-OC515 Polyclonal 0,5 ml / 100 test Coming Soon RUO Pathological human bone marrow sample stained with CYT-45OC.

9 Antibodies (Ab) 9 Single Color Antibodies against Human Antigens Description Clone Isotype Format Reference Use CD1a-fitc NA1/34-HLK IgG2a 1ml / 200 test CYT-1AF1 RUO CD2-fitc B-E2 IgG2b 1ml / 200 test CYT-2F5 CE / IVD CD3-CF TM Blue GA60 IgG1 0,5ml / 100 test CYT-3CFB RUO CD3-fitc Cris-7 IgG2a 1ml / 200 test CYT-3F8 CE / IVD CD3-PE 33-2A3 IgG2a 1ml / 200 test CYT-3PE5 CE / IVD CD3-pecy5 UCHT1 IgG1 1ml / 200 test CYT-3C4 CE / IVD CD3-APC 33-2A3 IgG2a 1ml / 200 test CYT-3AP5 RUO CD3-APC GA60 IgG1 1ml / 200 test CYT-3AP6 RUO CD4-CF TM Blue H2P/6 IgG2a 0,5ml / 100 test CYT-4CFB RUO CD4-fitc Edu-2 IgG2a 1ml / 200 test CYT-4F6 CE / IVD CD4-PE Edu-2 IgG2a 1ml / 200 test CYT-4PE9 CE / IVD CD4-PerCP-Cy5.5 SK3 IgG1 0,5ml / 100 test CYT-4C5 RUO CD4-pecy5 13B8.2 IgG1 1ml / 200 test CYT-4C3 CE / IVD CD4-APC HP2/6 IgG2a 1ml / 200 test CYT-4AP3 RUO CD5-fitc MCD5 IgG2b 1ml / 200 test CYT-5F4 CE / IVD CD5-PE MCD5 IgG2b 1ml / 200 test CYT-5PE4 RUO CD5-PerCP-Cy5.5 UCHT2 IgG1 0,5ml / 100 test CYT-5C RUO CD7-fitc B-B7 IgG1 1ml / 200 test CYT-7F5 RUO CD7-pecy5 CD7-6B7 IgG2a 1ml / 200 test CYT-7C4 RUO CD7-APC CD7-6B7 IgG2a 1ml / 100 test CYT-7AP3 RUO CD7-APC HULY-M2 IgG2a 1ml / 200 test CYT-7AP4 RUO CD8-fitc UCH-T4 IgG2a 1ml / 200 test CYT-8F8 CE / IVD CD8-PE 17D8 IgG1 1ml / 200 test CYT-8PE4 CE / IVD CD8-pecy5 B9.11 IgG1 1ml / 200 test CYT-8C2 CE / IVD CD8-APC 17D8 IgG1 1ml / 200 test CYT-8AP RUO CD10-fitc SN5c IgG1 1ml / 200 test CYT-10F1 RUO CD10-PE FR4D11/F3 IgG1 1ml / 100 test CYT-10PE9 RUO CD11c-PE B-ly6 IgG1 1ml / 200 test CYT-11cPE3 CE / IVD

10 10 Antibodies (Ab) Description Clone Isotype Format Reference Use CD13-fitc 22A5 IgG2a 1ml / 100 test CYT-13F2 RUO CD13-PE WM15 IgG1 1ml / 100 test CYT-13PE3 RUO CD14-fitc 18D11 IgG1 1ml / 200 test CYT-14F4 CE / IVD CD14-PE 47-3D6 IgG1 1ml / 200 test CYT-14PE3 RUO CD14-APC 47-3D6 IgG1 1ml / 200 test CYT-14AP3 RUO CD15-Purified MCS-1 IgG3 1ml / 100 test CYT-15P3 RUO CD15-fitc MCS-1 IgG3 1ml / 100test CYT-15F4 CE / IVD CD16-fitc LD16 IgG2a 1ml / 200 test CYT-16F4 CE / IVD CD16-PE LD16 IgG2a 1ml / 200 test CYT-16PE6 CE / IVD CD16+56-PE LD16 + C5.9 IgG2a+IgG2b 1ml / 100 test CYT-16PE6+56PE CE / IVD CD19-fitc HD37 IgG1 1ml / 200 test CYT-19F4 CE / IVD CD19-PE HD37 IgG1 1ml / 200 test CYT-19PE5 CE / IVD CD19-pecy5 J4.119 IgG1 1ml / 200 test CYT-19C2 CE / IVD CD20-CF TM Blue LT20 IgG2a 0,5ml / 100 test CYT-20CFB RUO CD20-fitc 2H7 IgG2b 1ml / 200 test CYT-20F3 CE / IVD CD20-PE B-ly1 IgG1 1ml / 200 test CYT-20PE5 RUO CD20-APC B-ly1 IgG1 1ml / 200 test CYT-20AP RUO CD22-fitc B-ly8 IgG1 1ml / 200 test CYT-22F3 RUO CD22-PE Mc64-12 IgG1 1ml / 200 test CYT-22PE5 RUO CD23-PE B-G6 IgG1 1ml / 200 test CYT-23PE3 RUO CD24-PE ML5 IgG2a 1ml / 100 test CYT-24PE2 RUO CD33-fitc WM53 IgG1 1ml / 100 test CYT-33F CE / IVD CD33-PE WM53 IgG1 1ml / 200 test CYT-33PE3 RUO CD36-fitc SMO IgM 1ml / 200 test CYT-36F CE / IVD CD38-Purified LD38 IgG1 1ml / 100 test CYT-38P1 RUO CD38-fitc LD38 IgG1 1ml / 200 test CYT-38F RUO CD38-PE T16 IgG1 1ml / 200 test CYT-38PE2 RUO CD41-fitc PM6/248 IgG1 1ml / 200 test CYT-41F RUO CD43-fitc DFT1 IgG1 1ml / 200 test CYT-43F2 RUO CD43-PE DFT1 IgG1 1ml / 200 test CYT-43PE2 RUO CD45-OC515 GA90 IgG2a 0,5ml / 100 test CYT-45OC RUO CD45-fitc EO1 IgG2a 1ml / 200 test CYT-45F5 CE / IVD CD45-PE D3/9 IgG1 1ml / 200 test CYT-45PE4 CE / IVD CD45-pecy5 IMMU19.2 IgG1 1ml / 200 test CYT-45C3 CE / IVD CD45-APC ML2 IgG1 1ml / 200 test CYT-45AP2 RUO

11 Antibodies (Ab) 11 Description Clone Isotype Format Reference Use CD49d-fitc 44H6 IgG1 1ml / 100 test CYT-49dF RUO CD52-PE YTH34.5 IgG2b 1ml / 200 test CYT-52PE RUO CD55-fitc IgG1 1ml / 100 test CYT-55F6 RUO CD55-PE NaM16-4D3 IgG1 1ml / 200 test CYT-55PE3 RUO CD56-fitc C5.9 IgG2b 1ml / 200 test CYT-56F CE / IVD CD56-PE C5.9 IgG2b 1ml / 200 test CYT-56PE CE / IVD CD59-fitc MEM-43 IgG2a 1ml / 200 test CYT-59F6 RUO CD59-PE NaM172-2B5 IgG1 1ml / 200 test CYT-59PE7 RUO CD64-PE 10.1 IgG1 1ml / 200 test CYT-64PE RUO CD65-fitc 88H7 IgM 1ml / 200 test CYT-65F RUO CD66b-fitc 80H3 IgG1 1ml / 200 test CYT-66bF RUO CD79a-fitc SP18 IgG1 1ml / 200 test CYT-79aF3 RUO cycd79a-pe HM57 IgG1 0,5ml / 100 test CYT-79aPE RUO CD103-fitc B-ly7 IgG1 1ml / 200 test CYT-103F2 RUO CD103-PE LF61 IgG1 1ml / 200 test CYT-103PE RUO CD117-fitc 104D2 IgG1 1ml / 100 test CYT-117F4 RUO CD117-PE 104D2 IgG1 1ml / 100 test CYT-117PE2 RUO CD138-Purified B-A38 IgG1 1ml / 100 test CYT-138P4 RUO CD138-fitc B-A38 IgG1 1ml / 200 test CYT-138F4 RUO CD138-PE B-A38 IgG1 1ml / 200 test CYT-138PE4 RUO CD138-pecy5 B-A38 IgG1 1ml / 200 test CYT-138C4 RUO HLADR-fitc 423L IgG2a 1ml / 200 test CYT-DRF RUO HLADR-PE 423L IgG2a 1ml / 200 test CYT-DRPE RUO HLA B27-fitc HLA ABC m3 IgG2a 1ml / 200 test CYT-HLAIB27F RUO HLA B7-PE BB7.1 IgG1 1ml / 200 test CYT-HLAIB7PE RUO Kappa-fitc Polyclonal 0,5ml / 100 test CYT-KAPPF2 CE / IVD Kappa-PE Polyclonal 0,5ml / 100 test CYT-KAPPPE RUO Lambda-fitc Polyclonal 0,5ml / 100 test CYT-LAMBF RUO Lambda-PE Polyclonal 0,5ml / 100 test CYT-LAMBPE RUO cytmpo-fitc 8E6 IgG1 1ml / 100 test CYT-MPOF2 RUO FMC7-fitc FMC7 IgM 1ml / 200 test CYT-FMC7F RUO TdT-fitc Polyclonal 0,5ml / 50 test CYT-TdTF RUO ZAP70-PE 1E7.2 IgG1 1ml / 100 test CYT-ZAP70PE3 RUO Citokeratin 18 - fitc Ks18.04 IgG1 0,250 ml CYT-CK18F RUO cytcrß-apc 8A3 IgG1 0,5ml / 100 test CYT-bF1AP RUO

12 12 Antibodies (Ab) Double Color Combinations Description Clone Format Reference Use CD3-fitc CD4-PE Cris-7 / Edu-2 1ml / 100 test CYT-3F8-4PE9 CE / IVD CD3-fitc CD8-PE Cris-7 / 17D8 1ml / 100 test CYT-3F8-8PE4 CE / IVD CD3-fitc CD16-PE Cris-7 / LD16 1ml / 100 test CYT-3F8-16PE6 CE / IVD CD3-fitc CD16+CD56 PE Cris-7/ LD16 + C5.9 1ml / 100 test CYT-3F8-16PE6+56PE CE / IVD CD3-fitc CD19-PE Cris-7/ HD37 1ml / 100 test CYT-3F8-19PE5 CE / IVD CD3-fitc CD56-PE Cris-7 / C5.9 1ml / 100 test CYT-3F8-56PE CE / IVD CD3-fitc HLADR-PE Cris-7 / 423L 1ml / 100 test CYT-3F8-DRPE RUO CD4-fitc CD8-PE Edu-2 / 17D8 1ml / 100 test CYT-4F6-8PE4 CE / IVD CD4-fitc CD45-PE Edu-2 / D3/9 1ml / 100 test CYT-4F6-45PE4 CE / IVD CD5-fitc CD19-PE MCD5 / HD37 1ml / 100 test CYT-5F4-19PE5 CE / IVD CD8-fitc CD16-PE UCH-T4 / LD16 1ml / 100 test CYT-8F8-16PE6 CE / IVD CD8-fitc CD56-PE UCH-T4 / C5.9 1ml / 100 test CYT-8F8-56PE CE / IVD CD45-fitc CD14PE EO1 / 47-3D6 1ml / 100 test CYT-45F5-14PE3 CE / IVD HLA-B27 fitc HLA-B7 PE HLA ABC m3 / BB7.1 1ml / 100 test CYT-COMB1012 RUO Kappa fitc Lambda-PE Polyclonal 0,5ml / 50 test CYT-KF2-LPE RUO Lambda fitc Kappa-PE Polyclonal 0,5ml / 50 test CYT-LF-KPE RUO Triple Color Combinations Description Clone Format Reference Use CD3-FITC CD4-PE CD8-pecy5 Cris-7 / Edu-2 / B9.11 1,5ml / 100 test CYT-3F8-4PE9-8C2 CE / IVD CD3-fitc CD4-PE CD19-pecy5 Cris-7/ Edu-2 / J ,5ml / 100 test CYT-3F8-4PE9-19C2 CE / IVD CD3-fitc CD4-PE CD45-pecy5 Cris-7/ Edu-2 / IMMU19.2 1,5ml / 100 test CYT-3F8-4PE9-45C3 CE / IVD CD3-fitc CD8-PE CD19-pecy5 Cris-7 / 17D8 / J ,5ml / 100 test CYT-3F8-8PE4-19C2 CE / IVD CD3-fitc CD8-PE CD45-pecy5 Cris-7 / 17D8 / IMMU19.2 1,5ml / 100 test CYT-3F8-8PE4-45C3 CE / IVD CD3-fitc CD16+56-PE CD19-pecy5 Cris-7 / LD16 + C5.9 / J ,5ml / 100 test CYT-3F8-16PE6+56PE-19C2 CE / IVD CD3-fitc CD16+56-PE CD45-pecy5 Cris-7 / LD16 + C5.9 / IMMU19.2 1,5ml / 100 test CYT-3F8-16PE6+56PE-45C3 CE / IVD

13 Antibodies (Ab) 13 Description Clone Format Reference Use CD3-FITC CD16-PE CD19-pecy5 Cris-7 / LD16 / J ,5ml / 100 test CYT-3F8-16PE6-19C2 CE / IVD CD3-fitc CD19-PE CD45-pecy5 Cris-7 / HD37 / IMMU19.2 1,5ml / 100 test CYT-3F8-19PE5-45C3 CE / IVD CD3-fitc CD56-PE CD19-pecy5 Cris-7 / C5.9 / J ,5ml / 100 test CYT-3F8-56PE-19C2 CE / IVD CD4-fitc CD8-PE CD3-pecy5 Edu-2 / 17D8 / UCHT1 1,5ml / 100 test CYT-4F6-8PE4-3C4 CE / IVD CD4-fitc CD8-PE CD45-pecy5 Edu-2 / 17D8 / IMMU19.2 1,5ml / 100 test CYT-4F6-8PE4-45C3 CE / IVD CD8-fitc CD4-PE CD3-pecy5 UCH-T4 / Edu-2 / UCHT1 1,5ml / 100 test CYT-8F8-4PE9-3C4 CE / IVD CD8-fitc CD4-PE CD19-pecy5 UCH-T4 / Edu-2 / J ,5ml / 100 test CYT-8F8-4PE9-19C2 CE / IVD CD8-fitc CD4-PE CD45-pecy5 UCH-T4 / Edu-2 / IMMU19.2 1,5ml / 100 test CYT-8F8-4PE9-45C3 CE / IVD CD10-fitc CD5-PE CD19-pecy5 SN5c / MCD5 / J ,5ml / 100 test CYT-10F1-5PE4-19C2 RUO CD16-fitc CD19-PE CD3-pecy5 LD16 / HD37/ UCHT1 1,5ml / 100 test CYT-16F4-19PE5-3C4 CE / IVD CD56-fitc CD19-PE CD3-pecy5 C5.9 / HD37 / UCHT1 1,5ml / 100 test CYT-56F-19PE5-3C4 CE / IVD FMC7-fitc CD23-PE CD19-pecy5 FMC7 / B-G6 7 J ,5ml / 100 test CYT-FMC7F-23PE3-19C2 RUO Kappa-fitc Lambda-PE CD19-pecy5 Polycl. / Polycl. / J test CYT-KF2-LPE-19C2 RUO Kappa-fitc Lambda-PE CD19-pecy5 Polycl. / Polycl. / J test CYT-KF2-LPE-19C2-50 RUO Lambda-fitc Kappa-PE CD19-pecy5 Polycl. / Polycl. / J test CYT-LF-KPE-19C2 RUO Other Combinations Description Format Reference Use CD8+Lambda-FITC CD56+Kappa-PE 25 tests CYT-SLPC-25 RUO CD8+Lambda-fitc CD56+Kappa-PE 50 tests CYT-SLPC-50 RUO CD8+Lambda-fitc CD56+Kappa-PE CD4-PerCP-Cy tests CYT-SLPC4-25 RUO CD8+Lambda-fitc CD56+Kappa-PE CD4-PerCP-Cy tests CYT-SLPC4-50 RUO

14 14 Antibodies (Ab) Isotype Controls Description Clone Format Reference Use IgG1-fitc MCG1 1 ml / 200 test CYT-IC005F CE / IVD IgG1-PE MCG1 1 ml / 200 test CYT-IC005PE CE / IVD IgG1-pecy5 ZX3 1 ml / 200 test CYT-IC005C CE / IVD IgG1-APC MCG1 1ml / 200 test CYT-IC005AP RUO IgG2a-fitc MCG2a 1 ml / 200 test CYT-IC006F CE / IVD IgG2a-PE MCG2a 1 ml / 200 test CYT-IC006PE CE / IVD IgG2b-fitc MCG2b 1 ml / 200 test CYT-IC007F CE / IVD IgG2b-PE MCG2b 1 ml / 200 test CYT-IC007PE CE / IVD IgG1-fitc IgG1-PE MCG1 / MCG1 1 ml / 100 test CYT-IC5F5PE CE / IVD IgG1-fitc IgG2a-PE MCG1 / MCG2a 1 ml / 100 test CYT-IC5F6PE CE / IVD IgG1-fitc IgG2b-PE MCG1 / MCG2b 1 ml / 100 test CYT-IC5F7PE CE / IVD IgG2a-fitc IgG1-PE MCG2a / MCG1 1 ml / 100 test CYT-IC6F5PE CE / IVD IgG2a-fitc IgG2a-PE MCG2a / MCG2a 1 ml / 100 test CYT-IC6F6PE CE / IVD IgG2a-fitc IgG2b-PE MCG2a / MCG2b 1 ml / 100 test CYT-IC6F7PE CE / IVD IgG2b-fitc IgG1-PE MCG2b / MCG1 1 ml / 100 test CYT-IC7F5PE CE / IVD IgG2b-fitc IgG2a-PE MCG2b / MCG2a 1 ml / 100 test CYT-IC7F6PE CE / IVD IgG2b-fitc IgG2b-PE MCG2b / MCG2b 1 ml / 100 test CYT-IC7F7PE CE / IVD IgG1-fitc IgG1-PE IgG1-pecy5 MCG1 / MCG1 / ZX3 1,5ml / 100 test CYT-IC5F5PE5C CE / IVD IgG1-fitc IgG2a-PE IgG1-pecy5 MCG1 / MCG2a / ZX3 1,5ml / 100 test CYT-IC5F6PE5C CE / IVD IgG1-fitc IgG2b-PE IgG1-pecy5 MCG1 / MCG2b / ZX3 1,5ml / 100 test CYT-IC5F7PE5C CE / IVD IgG2a-fitc IgG1-PE IgG1-pecy5 MCG2a / MCG1 / ZX3 1,5ml / 100 test CYT-IC6F5PE5C CE / IVD IgG2a-fitc IgG2a-PE IgG1-pecy5 MCG2a / MCG2a / ZX3 1,5ml / 100 test CYT-IC6F6PE5C CE / IVD IgG2a-fitc IgG2b-PE IgG1-pecy5 MCG2a / MCG2b / ZX3 1,5ml / 100 test CYT-IC6F7PE5C CE / IVD IgG2b-fitc IgG1-PE IgG1-pecy5 MCG2b / MCG1 / ZX3 1,5ml / 100 test CYT-IC7F5PE5C CE / IVD IgG2b-fitc IgG2a-PE IgG1-pecy5 MCG2b / MCG2a / ZX3 1,5ml / 100 test CYT-IC7F6PE5C CE / IVD IgG2b-fitc IgG2b-PE IgG1-pecy5 MCG2b / MCG2b / ZX3 1,5ml / 100 test CYT-IC7F7PE5C CE / IVD

15 Reagents: Erythrocyte Lysing Solution 15 Quicklysis TM Quicklysis TM is an erythrocyte lyse-non-wash method without fixatives that allows the study of leukocyte subpopulations by flow cytometry. It is used in whole blood or bone marrow samples after immunofluorescence staining of the cells, and requires no subsequent washing-centrifugation steps therefore reducing the processing time and sample handling. The use of Quicklysis TM avoids cell losses associated to the fixation and washing steps, maintaining the relative distribution of the cellular subsets present in the sample. This feature is specially important in absolute counts of CD34+ progenitor cells studies. Name Reference Format Use Quicklysis TM CYT-QL-1 Liquid form ready to use of 250 test CE / IVD

16 16 Reagents for Lymphocyte Subsets Studies CYTOGNOS offers unique, innovative, simple and easy to use antibody combinations, which reduce tube handling and simplify the analysis process. Lymphogram Lymphoclonal TM Lymphogram Lymphogram is a mixture of monoclonal antibodies with fluorescence intensities carefully selected that allows a clear separation of different lymphocyte subpopulations in a single tube. This reagent contains the following antibodies combination in a single vial: CD8 + CD19-FITC CD3 + CD56-PE CD4-PECy5 T-CD8+ CD8+/CD4+ CD4 /CD8 T-CD4+ T Cells NK Cells B Cells CD56/CD8 CD56/CD8+ Name Reference Format Use Lymphogram CYT-C TEST CE / IVD

17 Reagents 17 Lymphoclonal TM Lymphoclonal TM represents a rapid, effective and reliable tool for initial screening of lymphocyte analysis since it determines the levels of a full range of cell subsets and helps to establish the diagnosis of B-lymphocyte clonality in a single tube. This reagent contains the following antibodies combination in a single vial: LYMPHOCLONAL TM Lambda + CD8 - FITC Kappa + CD56 - PE CD4 + CD19 PerCP-Cy5.5 CD3 - APC LYMPHOCLONAL TM COULTER Lambda + CD8 - fitc Kappa + CD56 - PE CD4 + CD19 pecy5 CD3 pecy7 whole sample b lymphocyte subsets t lymphocyte subsets NK cell subsets Name Reference Format Use Lymphoclonal CYT-LC 20 TEST CE / IVD Lymphoclonal -C CYT-LC-C 20 TEST RUO

18 18 Reagents for DNA Content and Cell Cycle Analysis Cycloscope TM is an exclusive product line for flow cytometric cell DNA content measurements in patients with different diseases. The detection of DNA aneuploidies and cell cycle phase study offers relevant clinical information for prognostic evaluation and follow-up of patients with solid tumors or hematological malignancies. It has been demonstrated for some type of diseases that the existence of DNA aneuploidy is characteristic of malignant neoplasms and depending on the type of disease can be associated with better or worse prognosis. A high proliferative rate of tumor cells is usually associated to poor clinical outcome and helps to evaluate disease progression. The main advantage of this product line is that allows the measurement of cell DNA content on tumor cells specifically identified on basis of their phenotype. Cycloscope TM kits include a specific pool of monoclonal antibodies, to increase the ability to identify the neoplastic cells and separate them from other normal cells present in the sample, and a ready to use Propidium Iodide Solution for DNA analysis. This patented double staining method is sensitive and specific, allowing the simultaneous assessment of antigenic stainings and cell DNA in a single tube. Cycloscospe TM MM: Kit focused on the measurement of plasma cell DNA content in Multiple Myeloma (MM) and Monoclonal Gammopathies of Undetermined Significance (MGUS). Cycloscope TM B ALL: Suitable for the study of DNA content on neoplastic B-cell precursors in B-lineage Acute Lymphoblastic Leukemia (B-ALL). Cycloscope TM NHL: Kit focused on the measurements of cell DNA content on neoplastic B cells in B-lineage Non- Hodgkin Lymphoma (B-NHL) and other B-cell Chronic Lymphoproliferative Diseases (B-CLPD). Cycloscope TM SBC: Developed for the screening of bladder cancer in urine samples through the DNA content study of epithelial cells. Cycloscope TM Reagent: Kit containing a Propidium Iodide solution ready to use, and an erythrocyte lysing solution. Name Reference Format Use Cycloscope TM Reagent CYT-CS-R Test RUO DNA labelling Solution: Propidium Iodide solution ready to use, containing Propidium Iodide, permeabilization reagents and RNAse. Name Reference Format Use DNA labelling Solution CYT-PIR Test RUO

19 Reagents 19 Cycloscope TM MM (Multiple Myeloma) It has been described that the number of bone marrow plasma cells in S-phase in patients with MM is the most important independent prognostic factor, allowing the discrimination of two subgroups of patients with different prognosis, even within the same clinical stage. Also the analysis of DNA aneuploidy has been considered to be a powerful tool for the detection of minimal residual disease in these patients. Cycloscope TM MM is a kit that allows the identification of plasma cells and their subsequent specific analysis of DNA measurements in patients with Multiple Myeloma (MM) or Monoclonal Gammopathies of Undetermined Significance (MGUS). This kit includes a mixture of anti human CD38 and CD138 antibodies to identify plasma cells. Cycloscope TM MM includes the following components: Anti human CD38 and CD138 Purified Antibodies. Secondary Antibody FITC conjugated. Erythrocyte Lysing Solution. DNA Labeling Solution ready to use. G0/G1 Normal cells G0/G1 Hyperdiploid cells Name Reference Format Use Cycloscope MM CYT-CS-MM 20 TEST RUO

20 Reagents 20 Cycloscope TM B-ALL (B Acute Lymphoblastic Leukemia) Cycloscope TM B ALL is a kit for the measurement of cell DNA content on neoplastic B-cells precursors in B-lineage acute lymphoblastic leukemia (B-ALL). Detection of hyperdiploid leukemic blast cells has been described as independent prognostic factor strongly associated with favourable clinical and biological features. Therefore, DNA index offers a practical and reproducible way to characterise B-ALL patients contributing to the establishment of risk-directed treatment strategies. In addition, DNA studies may be of great utility for the detection of minimal residual disease and contribute therefore to relapses prediction in these patients. Cycloscope TM B-ALL contains: Anti human CD10+ CD19 + CD20 + CD22 Purified Antibodies. Secondary Antibody FITC conjugated. Erythrocyte Lysing Solution. DNA Labeling Solution ready to use. normal diploid cells Blast cells residual b Cells G0/G1 hyperdiploid G0/G1 diploid Synthesis /G2-M hyperdiploid Name Reference Format Use Cycloscope B-ALL CYT-CS-BALL 20 TEST RUO

21 Reagents 21 Cycloscope TM NHL (B-Non Hodgkin Lymphoma and other B-CLPD) Cycloscope TM NHL is a kit focused on the measurement of cell DNA content on neoplastic B-cells precursors in B-lineage Non Hodgkin s Lymphoma (B-NHL) and other B-cell Chronic Lymphoprolipherative Disorders (B-CLPD). Previous studies have reported that the percentage of tumoral B-cells in S-phase represents a prognostic factor either in B-NHL or B-CLL. Additionally, it has been observed that in B-NHL patients, DNA-ploidy correlates with histopathologic grade. The kit includes: Anti human CD19 + CD20 + CD22 + CD23 Purified Antibodies. Secondary Antibody FITC conjugated. Erythrocyte Lysing Solution. DNA Labeling Solution ready to use. G 0 /G 1 S+G 2 M diploid CeLls pathological cells Name Reference Format Use Cycloscope TM NHL CYT-CS-NHL 20 TEST RUO

22 Reagents 22 Cycloscope TM SBC (Screening Bladder Cancer) Cycloscope TM SBC is a reagent for the screening of bladder cancer in urine samples, which combines the use of specific markers of epithelial cells (AE1 + AE3) with DNA staining. This double staining method (cytokeratin / DNA) allows the identification of epithelial cells and the comparative analysis of DNA measurements between epithelial and other normal cells present in the sample. The presence of DNA Aneuploidy and/or a high proliferative rate of tumor cells are associated with malignant histology and poor clinical outcome. Cycloscope TM SBC contains: Anti human AE1 and AE3 Purified Antibodies. Secondary Antibody FITC conjugated. DNA Labeling Solution ready to use. A B Select the epithelial cells according to high FL1-FITC fluorescence intensity Gate cells excluding the cellular agregates NORMAL cells Epithelial cells Name Reference Format Use Cycloscope TM SBC CYT-CS-SBC 20 TEST RUO

23 Reagents for Monitoring Monoclonal Gammopathies 23 Clonalpath TM y Clonalpath TM Plus Clonalpath TM is the ideal tool for monitoring of MM and MGUS patients based on the quantification of bone marrow plasma cells. Normal plasma cells typically display strong reactivity for the CD38 antigen, intermediate FSC/SSC pattern, co-express the CD19 and CD45 antigen while they are generally negative for the CD56 antigen. The presence of immunophenotypically normal plasma cells, which is a constant finding in MGUS, is a rare event in MM and when it is present, its frequency is significantly lower (<3%) than that observed in MGUS. Pathological plasma cells can be distinguished from normal plasma cells based in their different immunophenotypic pattern of expression for the antibodies anti CD19, CD56, CD45 and CD38. Normal PC Pathological PC CD19 + (96%) CD56 + (67%) CD / ++ (45%) CD (100%) Pérez-Andrés et al. J Bio Regul Agent 2004; 18: Clonalpath TM (CD38-FITC / CD56-PE / CD19-PECy5) and Clonalpath TM -Plus (CD38-FITC / CD56-PE / CD19-PECy5 / CD45-APC) are two combinations of antibodies useful for the identification and characterization of plasma cells present in the sample. mgus patient pathological plasma cells normal plasma cells Name Reference Format Use Clonalpath CYT-CP TEST RUO Clonalpath -Plus CYT-CP45 50 TEST RUO

24 24 Reagent for Dendritic Cell Studies As a major component of the immune system, dendritic cells (DCs) have a critical role in stimulating a tumor-specific immune response. This stimulation is of great interest for its application in immunological-based therapies, such as antitumor cell vaccination with autologous DCs. Therefore, as immunotherapy using these cells has the potential to become a treatment option, it is important to have a greater understanding of the number and function of DCs and to know the optimal conditions for their collection and administration. Most of the studies reveal the difficulties in defining the distinct DCs populations. Dendritic Cells Exclusion Kit is a CD3+CD19+CD56+CD14 antibody combination conjugated with FITC designed to identify by exclusion DCs. Peripheral blood DCs can be distinguished from other leucocyte subsets by their characteristic lack of staining with this antibody combination and a pattern of FSC / SSC intermediate between lymphocytes and monocytes. The combined use of this reagent with other antibodies such as anti human HLA DR, allows a clear identification of total dendritic cells population. Additionally, it has been reported that the use of this dendritic cells exclusion kit combined with anti-human CD33 antibody, anti human CD123 and/or anti-human CD16 allows the identification of different subpopulations of dendritic cells. Dendritic cells Name Reference Format Use DCE Kit CYT-DENDF 50 TEST RUO

25 Absolute Counting Systems 25 The absolute counting of cell subpopulations by means of flow cytometry can be done using a double platform technique (in which the information provided by the flow cytometer and by a haematological counter are combined), or using a single platform technique for which only the flow cytometer is used. The microbead-based single platform technology has emerged as the method of choice for absolute cell enumeration in different clinical applications due to the accuracy of the results. Perfect-Count Microspheres TM Perfect-Count Microspheres TM is a microbead-based single platform system, which assures the accuracy of absolute count results. Its unique internal quality control system contains two types of beads with densities around the upper and lower densities of peripheral blood cells. Variations of the ratio between beads type A and B warn about problems during sample preparation and/or acquisition which could invalidate the final results. CD34+ Beads A Beads B CD4+ CD8+ CD34 cell counts Beads A and Beads B CD4-CD8 cell counts Name Reference Format Use Perfect-Count Microspheres CYT-PCM test CE/IVD Perfect-Count Microspheres CYT-PCM test CE/IVD

26 26 Reagents LeucoFinder TM LeucoFinder TM is an efficient single-platform method for the enumeration of residual white blood cells (rwbc) in leucoreduced blood products for transfusion. An accurate rwbc absolute count in filtered blood products for transfusion is critical to prevent febrile reactions, micro organism transfer, alloimmunization, platelet refractoriness and immunosuppression. LeucoFinder TM combines the detection of residual leukocytes by DNA labeling of their nucleus with the use of Perfect-Count Microspheres TM as absolute count system that provides a unique method of internal quality control. rwbc Beads B Beads A Beads A Beads B Name Reference Format Use LeucoFinder CYT-LF test CE / IVD

27 Screening Kit for Paroxismal Nocturnal Hemoglobinuria (PNH) 27 Paroxismal Nocturnal Hemoglobinuria (PNH) is an acquired clonal hematopoietic stem-cell disorder related to the occurrence of a somatic mutation in the PIG-A gen, located in the human X chromosome. This genetic alteration results in a partial or total deficiency of all proteins normally linked to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor (GPI-anchored proteins). Flow cytometry provides a direct diagnosis tests in which the lack of GPI anchored proteins is studied on the major cell populations present in blood. The screening kit for PNH includes the following antibody combination: CD16-FITC / CD64-PE / CD45-PerCP-Cy5.5 / CD14-APC. With this antibody combination, it is possible to detect the existence of GPI deficient cells with a 100% of sensitivity. Antibodies anti-cd45 and anti-cd64 allow the identification of the populations to be studied: neutrophils and monocytes. The further evaluation of a possible loss of expression of the CD16 molecule on neutrophils and loss of expression of the CD14 molecule on monocytes allows the identification of the PNH clone in a fast and simple way. monocytes HPN Monocytes Normal Monocytes PNH Neutrophils neutrophils Normal Neutrophils Name Reference Format Use HPN Screening CYT-HPN-1 25 TEST CE / IVD

28 28 Flow Cytometry Data Analysis Infinicyt TM Flow Cytometry Data Analysis Software In our commitment to innovation and development, we continue incorporating new tools to Infinicyt TM, the most advanced software for data integration and multidimensional analysis of flow cytometry files. Digital Flow cytometers are incorporating the availability of increasing the number of parameters that can simultaneously be assessed in individual cells and the speed of analysis to thousands cells per second. However, generation of such amount of complex information also requires appropriate changes in the capabilities of data analysis and data representation. Infinicyt TM is the tool to integrate and manage all this information in a powerful, intuitive and simple way. New software tools for data analysis and data interpretation. Infinicyt TM offers increasingly software tools that provide flow cytometry users the possibility of designing a new strategy for data analysis, adapted to their own set of data files, without loosing the possibility of using conventional analytical approaches in parallel. In addition, it provides an extensive set of complementary tools to help expert-based interpretation of complex multiparameter data sets. The analysis and interpretation of results is now faster, easier and more accurate.

29 Software 29 The merging function, allows you integrating several FCS related files into a single file containing all parameters. The analysis is simplified, it is possible to gate the cell populations of interest simultaneously and visualize their complete immunophenotypic profile in a single diagram. In addition it is possible export the obtained results with the information provided by each file independently or with the global information of the merged file. 5 single files 1 merged file Infinicyt TM incorporates powerful algorithms that allow users a precise and reliable calculation of the markers expression for the populations of interest, based on phenotypically identical cells in which these parameters were measured directly. Information from different aliquots is integrated into the same data file, allowing you to display new information such as data measured in different files or markers conjugated with the same fluorochrome. This data integration tool contributes to simplify and facilitate the management and interpretation of complex information. Manual analysis module provides a wide range of options and advanced tools which makes of Infinicyt TM the most powerful and innovative flow cytometry data analysis software available on the market. Analysis Strategy You can design and save an analysis template and apply it later to other similar files obtaining complete information about graphs, gates, statistics and analysis procedures. Finally, you can ensure that all samples have exactly the same analysis, or you have the flexibility to have sample-specific modifications of certain gates without affecting analysis strategy capabilities.

30 30 Software Automatic population separator diagram (APS) This graphic representation offers an automatic separation of the events, in which the software tries to distinguish as many cell clusters as possible. It is also a useful tool to compare different types of samples and identify the phenotypic differences and similarities between cell populations. GROup 1 GRoup 2 Multiple possibilities of visualizing the data Parameter band diagrams, box-plot graphs, bivariate 3 or multiple dimensional dot plots with colored squares indicating mean values, density contour representations, APS, overlay histograms Only Infinicyt TM offers such a wide variety of possibilities to visualize your data.

31 Software 31 Use reference images in your analysis You do not need to retain in your head high amount of data or images, simply select your reference images and apply them on other files as guide to compare in your analysis. NORMAL pattern PATIENT SAMPLE Results Report Design your own results report with the information really useful: diagrams, statistics, texts, reference images, etc. Or simply save the analyzed file for further revisions. Create your own databases of various types of samples and compare a specific file with them. Results will become more accurate and objective. Other ceatures also available with Infinicyt TM : Export statistics in a format compatible with other data analysis or word processing programs. Edit the diagrams labels. Change the compensation set of acquired files, with the option of saving and applying the new compensation set to other files. Create new functions and virtual parameters. Visit our website where you will find more detailed information and you can download a demo version. Within the support and guarantee of the most valuable Euroflow research groups (

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