Flow Cytometry 2014 July 14-18

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1 July 14th Monday 8:00-12:00 LECTURES 8:00-8:15 REFRESHMENTS SPONSORED BY BECKMAN COULTER 8:15-8:30 Check-in & Welcome Dr. Judy Kjelstrom, UCD Biotech Program & Bridget McLaughlin, UCD 8:30-9:00 Introduction to Flow Cytometry 9:00-10:00 Inside the Cytometer: Fluidics, Optics and Electronics Jonathan Van Dyke, Technical Manager, UCD 10:00-10:30 Understanding Flow Cytometry Data 10:30-10:45 BREAK SPONSORED BY BECKMAN COULTER 10:45-11:15 Fluorescence 11:15-12:00 Reagents, Antibodies, and Fluorescent Probes 12:00-1:00 LUNCH 1:00-5:00 LECTURES 1:00-2:00 Compensation 2:00-2:45 Data Analysis and Gating Bridget McLaughlin, Technical Director, Flow Cytometry Core 2:45-3:15 BREAK 3:15-4:00 Controls 4:00-5:00 Applications

2 July 15th Tuesday 9:00-10:00 Characterization and Experimental Setup 10:00-10:30 Intracellular Detection 10:30-10:45 BREAK SPONSORED BY EMD MILLIPORE 10:45-11:45 Statistics 11:45-1:00 LUNCH 1:00-3:00 MoFlo Lab Lab Group # LSRII Lab Lab Group # Amnis Lab Lab Group # Cytek Lab Lab Group # :00-3:15 BREAK SPONSORED BY STRATEDIGM 3301F LSRII Lab Lab Group # MoFlo Lab Lab Group # Cytek Lab Lab Group # Amnis Lab Lab Group #4 2433

3 July 16th Wednesday 9:00-10:00 Troubleshooting 10:00-10:15 BREAK SPONSORED BY BECKMAN COULTER 10:15-11:15 Allied Technologies 11:15-12:00 Principles and Applications of Amnis Imaging Flow Cytometry Dr. David Basiji, CEO Amnis Corp. 12:00-1:00 LUNCH LUNCH SPONSORED BY BIORAD 1:00-5:00 Amnis Lab Lab Group # Cytek Lab Lab Group # MoFlo Lab Lab Group # Cytek Lab Lab Group # :00-3:15 BREAK SPONSORED BY ebioscience 3301F Cytek Lab Lab Group # FlowJo Lab Lab Group # Cytek Lab Lab Group # MoFlo Lab Lab Group #4 3425

4 July 17th Thursday 9:00-10:00 Multicolor Panel Design 10:00-10:15 BREAK SPONSORED BY FLUIDIGM 10:15-11:00 Practical Panel Design Bridget McLaughlin, Technical Director, & Jonathan Van Dyke, Technical Manager, UCD 11:00-12:00 Proliferation Assays Jolene Bradford, MLS(ASCP) CM, CCy Associate Director R&D, Thermo Fisher Scientific 12:00-1:00 LUNCH LUNCH SPONSORED BY TREE STAR 1:00-3:00 FlowJo Lab Lab Group # Fluidigm Lab Lab Group # Attune Lab Lab Group # BD Lab Lab Group # :00-3:15 BREAK SPONSORED BY THERMO FISHER SCIENTIFIC 3301F FlowJo Lab Lab Group # Attune Lab Lab Group # BioRad Lab Lab Group # BD Lab Lab Group #4 3425

5 July 18th Friday 8:30-12:00 LECTURES 8:30-9:30 Advanced Data Analysis 9:30-9:45 Excyte Online Resources 9:45-10:00 BREAK 10:00-11:00 Flow Cytometry Instruments and Applications Dr. Martin Bigos, Shared FACS Facility, Stanford University 11:00-12:00 TBA Dr. Holden Maeker, Director Human Immune Monitoring Center, Stanford School of Medicine 12:00-1:00 LUNCH LUNCH SPONSORED BY BD 1:00-3:00 Fluidigm Lab Lab Group # BioRad Lab Lab Group # BD Lab Lab Group # Attune Lab Lab Group # :00-3:15 BREAK 3301F Attune Lab Lab Group # BD Lab Lab Group # Fluidigm Lab Lab Group # BioRad Lab Lab Group # FINAL REMARKS & CERTIFICATES Dr. Judy Kjelstrom (UCD Biotech Program) 5: F & Bridget McLaughlin (UCD )

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