Catalogue. GEN-IAL Version 11/14

Transcription

1 Catalogue DNA-Extraction-Kits Page 2-9 PCR-Detection-Kits Page 9-19 Colour Compensation-Kits Page 9 GMO PCR-Kits Page 9-12 Microorganism PCR-Kits Page Animal species PCR-Kits Page 17 Allergen PCR-Kits Page 18 Plant PCR-Kits Page 18 Acrylic-Pipette-Rack Page 19 GEN-IAL Version 11/14

2 DNA-Extraction-Kits First-DNA all-tissue Kit The First-DNA all-tissue Kit is one single system that makes DNA extraction possible from various substrates such as blood, urine, semen, cell culture, tissue embedded in paraffin, hair, bones, stains, plant and animal tissue, mouse tails, food, bacteria, yeast, fungi etc. without the use of toxic substances. High yields of quality DNA can be obtained, the mean purity of the isolated DNA, determined by the A260/A280 ratio, is There is no loss of DNA by columns and the eluted DNA is well suited for PCR, sequencing, RFLP etc. Art. No.: D ( 10 preparations, trial kit ) D ( 50 preparations ) D (100 preparations ) D (500 preparations) L1DNA050 (extra Lysis buffer 1 for 50 preparations) L1DNA100 (extra Lysis buffer 1 for 100 preparations) - 2 -

3 DNA-Extraction-Kits Simplex Easy DNA Kit In 15 minutes DNA from bacteria and yeast The Simplex Easy DNA Kit is an extremely fast and easy DNA-Extraction from bacteria and yeasts. It is also approved for mouse tails and epithelial swaps. DNA is well suited for PCR, sequencing and more. DNA-Extraction in 5 steps 1. Centrifuge sample 2. Remove supernatant 3. Add Simplex Easy reagent 4. Incubate sample at 95 C 5. Centrifuge sample Advantages DNA-extraction in one single vessel No contamination risk No toxic solutions DNA-cleaning is possible Reasonable price Universal applications Low material and instrument costs Art. No.: SE 0010 ( 10 preparations, trial kit ) SE 0100 (100 preparations ) - 3 -

4 DNA-Extraction-Kits Simplex Easy Wine Kit In 40 minutes DNA from bacteria and yeast The Simplex Easy Wine Kit is an extremely fast and easy method for isolation of DNA from bacteria and yeasts out of wine. It is particularly suitable for detection of wine spoilage microorganisms like e.g. Dekkera bruxellensis, Oenococcus oeni or lactobacilli. DNA-Extraction in 6 steps: 1. Centrifuge sample 2. Purify sample by washing (removal of inhibitors) 3. Remove the washing solution after centrifugation 4. Add Simplex Easy Wine reagent 5. Incubate sample at 95 C 6. Centrifuge sample Advantages DNA-extraction in one single reaction vessel No inhibition by washing the sample No contamination risk High efficiency No loss of DNA Fast and easy handling Universal applications Low material and instrument costs Art. No.: SEW 0100 (100 preparations) WS 0100 (100 preparations) - 4 -

5 DNA-Extraction-Kits First-Magnetic Forensic Kit DNA-Extraction from forensic material suitable for automation The First-Magnetic Forensic Kit is developed for automatic genomic DNA-extraction from various forensic materials. DNA is well suited for STR, SNP, sequencing etc. swaps (buccal, mucosa, vagina, etc.) blood stains hair cigarette contact stains ( e.g. from glass, sticky tape, etc.) STR-analysis of cigarette paper (up) and cigarette filter (down) with genres MPX-2LF (SERAC) The method is based on biomagnetic separation of genomic/mitochondrial DNA: After preparing the lysate, the DNA is bound to magnetic beads. The rest of cell material and other contaminants is washed away. The isolated DNA is eluted in TE or H 2 O. The regular volume is 50 µl. Art. No.: MFOR 0010 ( 10 preparations, trial kit ) MFOR 0100 ( 100 preparations ) MFOR 0500 ( 500 preparations ) - 5 -

6 DNA-Extraction-Kits First-Magnetic Food Kit DNA-Extraction from food, feed and beverages suitable for automation The First-Magnetic Food Kit is developed for genomic DNA-extraction from various materials and is especially approved for very complex and highly processed products. The DNA is well suited for PCR, sequencing, etc. Applications: highly processed products: e.g. starch, lecithin, soy sauce, tomato puree beverage source materials: e.g. concentrates, fruit puree dairy products: e.g. milk and milk products feed: e.g. forage cereals, spent hops (treber), fattening feed The method is based on biomagnetic separation of genomic DNA. After preparing the lysate, the DNA is bound to magnetic beads. The rest of cell material and other contaminants is washed away. The isolated DNA is eluted in TE or H 2 O. The regular volume is 50 µl. Art. No.: FMF 0010 ( 10 preparations, trial kit ) FMF 0100 ( 100 preparations ) - 6 -

7 DNA-Extraction-Kits First-Magnetic Milk Kit DNA-Extraction from milk and milk products suitable for automation The First-Magnetic Milk Kit is developed for genomic DNA-Extraction especially from milk and milk products. The DNA is well suited for PCR, sequencing, etc. Applications: milk, raw milk yoghurt quark cheese, cream cheese pudding The method is based on biomagnetic separation of genomic DNA. After preparing the lysate, the DNA is bound to magnetic beads. The rest of cell material and other contaminants is washed away. The isolated DNA is eluted in TE or H 2 O. The regular volume is 50 µl. Art. No.: FMM 0010 ( 10 preparations, trial kit ) FMM 0100 ( 100 preparations ) - 7 -

8 DNA-Extraction-Kits First-Beer Magnetic DNA Kit microorganism-dna Extraction from beverages suitable for automation The First-Beer Magnetic DNA Kit is developed for bacterial and yeast DNA-Extraction especially from beverages. Applications: beer beer mixing drinks juices wines The method is based on biomagnetic separation of genomic DNA. After preparing the lysate, the DNA is bound to magnetic beads. The rest of cell material and other contaminants is washed away. The isolated DNA is eluted in TE or H 2 O. The regular volume is 50 µl. The DNA extracted with this Kit is suitable for all real-time PCR assays, e.g. First-Beer PCR Kits: P1 Hyb-Probe-Screening or First-Yeast PCR Kits. Art. No.: FBD 0010 ( 10 preparations, trial kit) FBD 0100 (100 preparations) First-Salmonella DNA-Extraction-buffer First-Salmonella DNA-extraction buffer for rapid thermic cell lysis after preenrichment according to 64 LFGB Art. No.: FSD 0100 ( 100 preparations ) - 8 -

9 Further DNA-Extraction-Kits Simplex Easy Spin DNA-Kit DNA-extraction from juices, concentrates and tomato products SES Simplex Easy Legionella DNA-Kit DNA-extraction of Legionella spp. from water and waste water SESL Reference material All-Screen reference material Reference material from soy and corn meal with p35s, Tnos, pat, bar, pfmv, CTP2-CP4EPSPS 500mg RF-6x-mix 500 mg PCR-Detection-Kits Colour Compensation Kits for Realtime PCR LC 2.0 Colour Compensation (LightCycler 2.0) Colour Compensation for FAM/HEX labelled TaqMan Duplex systems CCFH LC 480 Colour Compensation TaqMan (LightCycler 480) Colour Compensation for TaqMan labelled systems CCLCT gencontrol -GMO-Kits All PCR-Kits are available for different realtime PCR machines on request Real-time PCR, qualitative (FAM) First-Plant Plant in general, single copy, PPLANT incl. internal Inhibition-Control First-Plant Plant in general, single copy, PPLANT incl. internal Inhibition-Control CaMV Cauliflower Mosaik Virus RT-CaMV CaMV Cauliflower Mosaic Virus RT-CaMV Duplex Virus Duplex Cauliflower Mosaik Virus and RT-Duplex-Virus Figwort Mosaic Virus (FAM/HEX) Duplex Virus Duplex Cauliflower Mosaik Virus and RT-Duplex-Virus Figwort Mosaic Virus (FAM/HEX) p35s/t-nos Screening CaMVp35S and T-nos RTO-screen p35s/t-nos Screening CaMVp35S and T-nos RTO-screen p35s/t-nos Duplex- Screening CaMVp35S/T-nos -duplex PCR RTO-duplex-screen

10 p35s/t-nos Duplex- Screening CaMVp35S/T-nos -duplex PCR RTO-duplex-screen pat/bar Duplex- Screening pat/bar -duplex PCR RTO-pat/bar duplex pat/bar Duplex- Screening pat/bar -duplex PCR RTO-pat/bar duplex pfmv Screening P34S-FMV-PCR RTO-pFMV pfmv Screening P34S-FMV-PCR RTO-pFMV EPSPS Screening CTP2-CP4-EPSPS -PCR RTO-EPSPS EPSPS Screening CTP2-CP4-EPSPS -PCR RTO-EPSPS bar Screening Bar -PCR RTO-bar bar Screening Bar -PCR RTO-bar P-NOS-nptII Screening P-NOS-nptII -PCR RTO-pnos-nptII P-NOS-nptII Screening P-NOS-nptII -PCR RTO-pnos-nptII Cry1Ab/Ac / t-35s Duplex-Screening Cry1Ab/Ac / CaMV-35S-terminator RTO-Cry-t35Sduplex Cry1Ab/Ac / t-35s Duplex-Screening Cry1Ab/Ac / CaMV-35S-terminator RTO-Cry-t35Sduplex RT-triplex I p35s/t-nos/epsps RT-triplex I p35s/t-nos/epsps RT-triplex II p35s/t-nos/pfmv RT-triplex II p35s/t-nos/pfmv RT-triplex III p35s/t-nos/epsps and internal Control RT-triplex III p35s/t-nos/epsps and internal Control RT-triplex IV p35s/t-nos/pfmv and internal Control RT-triplex IV p35s/t-nos/pfmv and internal Control p35s/t-nos/ctp2-cp4epsps triplex PCR (FAM/HEX/CY5) p35s/t-nos/ctp2-cp4epsps triplex PCR (FAM/HEX/CY5) p35s/t-nos/pfmv triplex PCR (FAM/HEX/CY5) p35s/t-nos/pfmv triplex PCR (FAM/HEX/CY5) p35s/t-nos/epsps triplex PCR plus IC (FAM/HEX/ROX/CY5) p35s/t-nos/epsps triplex PCR plus IC (FAM/HEX/ROX/CY5) p35s/t-nos/pfmv triplex PCR plus IC (FAM/HEX/ROX/CY5) p35s/t-nos/pfmv triplex PCR plus IC (FAM/HEX/ROX/CY5) RT-Triplex I RT-Triplex I RT-Triplex II RT-Triplex II RT-Triplex III RT-Triplex III RT-Triplex IV RT-Triplex IV RR-Soya GTS (RoundupReady )-soya RT-RR RR-Soya GTS (RoundupReady )-soya RT-RR RR2-Soya MON89788 (RoundupReady2 )-soya RT-RR RR2-Soya MON89788 (Roundup Ready2 )-soya RT-RR A Soya A (LibertyLink )-soya RT-A2704-Soya A Soya A (LibertyLink )-soya RT-A2704-Soya A Soya A (LibertyLink )-soya RT-A5547-Soya-25 25

11 A Soya A (LibertyLink )-soya RT-A5547-Soya Triplex-Soya I Triplex PCR A / A / RT-Trip-soyI A2704/A5547/DP DP (FAM/HEX/CY5) Triplex-Soya I A2704/A5547/DP Triplex PCR A / A / DP (FAM/HEX/CY5) RT-Trip-soyI Triplex-Soya II Triplex PCR DP /BPS-CV127-9 RT-Trip-soyII DP305423/CV127/MON87701 / MON87701 (FAM/HEX/CY5) Triplex-Soya II Triplex PCR DP /BPS-CV127-9 RT-Trip-soyII DP305423/CV127/MON87701 / MON87701 (FAM/HEX/CY5) EH Potato EH (Amflora )-potato RT-Amflora EH Potato EH (Amflora )-potato RT-Amflora RT73-Canola RT73 (RoundupReady )-canola RT-RT RT73-Canola RT73 (RoundupReady )-canola RT-RT Triplex-Canola I Triplex PCR Ms8 / T45 / Rf3 RT-Trip-canola Ms8/T45/Rf3 (FAM/HEX/CY5) Triplex-Canola I Triplex PCR Ms8 / T45 / Rf3 RT-Trip-canola Ms8/T45/Rf3 (FAM/HEX/CY5) MON810-Maize MON810 (YieldGuard )-maize RT-Mon MON810-Maize MON810 (YieldGuard )-maize RT-Mon Bt176-Maize Bt 176 (Maximizer )-maize RT-Bt Bt176-Maize Bt 176 (Maximizer )-maize RT-Bt Bt11-Maize Bt 11-maize RT-Bt Bt11-Maize Bt 11-maize RT-Bt T25-Maize T25-maize RT-T T25-Maize T25-maize RT-T MON88017-Maize MON88017 (Rootworm )-maize RT-MON MON88017-Maize MON88017 (Rootworm )-maize RT-MON GA21-Maize GA21 (RoundupReady )-maize RT-GA GA21-Maize GA21 (RoundupReady )-maize RT-GA TC1507-Maize TC1507 (Herculex )-maize RT-TC TC1507-Maize TC1507 (Herculex )-maize RT-TC NK603-Maize NK603 (RoundupReady )-maize RT-NK NK603-Maize NK603 (RoundupReady )-maize RT-NK MIR604-Maize MIR604-maize RT-MIR MIR604-Maize MIR604-maize RT-MIR

12 MIR162-Maize MIR162-maize RT-MIR MIR162-Maize MIR162-maize RT-MIR GMO-Soy GMO-Corn RR-, RR2-, A , A soya RT-GMSOY and soya-reference (5 x 25 rxn) MON810-, T25-, Bt11-, Bt176-, RT-GMCORN MON88017-, GA21-, TC1507-maize and maize-reference (8 x 25 rxn) PCR-Detection-Kits gencontrol -GMO-Kits All PCR-Kits are available for different realtime PCR machines on request Real-time PCR, quantitative (FAM) p35s/ Soya Quant CaMVp35S Quantification in Soya Qp35S-Soya p35s/ Soya Quant CaMVp35S Quantification in Soya Qp35S-Soya p35s/ Maize Quant CaMVp35S Quantification in Maize Qp35S-Maize p35s/ Maize Quant CaMVp35S Quantification in Maize Qp35S-Maize RR-Soya Quant GTS (RoundupReady ) Soya QRR Quantification in Soya RR-Soya Quant GTS (RoundupReady ) Soya QRR Quantification in Soya RR2-Soya Quant MON89788 (RoundupReady2 ) Soya QRR Quantification in Soy RR2-Soya Quant MON89788 (RoundupReady2 ) Soya QRR Quantification in Soy MON810-Maize Quant MON810-Maize Quantification in Maize QMON MON810-Maize Quant MON810-Maize Quantification in Maize QMON Bt176-Maize Quant Bt176-Maize Quantification in Maize QBt Bt176-Maize Quant Bt176-Maize Quantification in Maize QBt Bt11-Maize Quant Bt11-Maize Quantification in Maize QBt Bt11-Maize Quant Bt11-Maize Quantification in Maize QBt T25-Maize Quant T25-Maize Quantification in Maize QT T25-Maize Quant T25-Maize Quantification in Maize QT

13 PCR-Detection-Kits Microorganisms All PCR-Kits are available for different realtime PCR machines on request Bacteria and yeast screening with differentiation First-Beer Differentiation PCR kit P1 Hyb Probe Screening LC 2.0 (FRET) P1 TaqMan Screening LC 480 (FAM, 610, 660, HEX) ABI 7500 (FAM, Bodipy, CY5, HEX) RotorGene (FAM, ROX, CY5, HEX) Spartan (FAM, ROX) Biorad (FAM, ROX) P1 TaqMan Screening LC 480 (FAM, 610, HEX) ABI 7500 (FAM, Bodipy, HEX) RotorGene (FAM, ROX, HEX) Spartan (FAM, ROX) Biorad (FAM, ROX) P1 TaqMan Screening Spartan (FAM, ROX) Multiplex detection and identification of beer spoilage microorganisms Multiplex PCR detection of beer-spoiling bacteria with yeast/without yeast (LC640, 610, 670, 705) MultiplexTaqMan-PCR detection of beer-spoiling bacteria and yeast MultiplexTaqMan-PCR detection of beer-spoiling bacteria without yeast MultiplexTaqMan-PCR detection of beer-spoiling bacteria,yeast and acetic acid bacteria TPBD PP1H 0050 LC 2.0/ PP1HoH 0050 LC 2.0 PP1T 0050 LC 480 PP1T 0048 ABI 7500 PP1T 0050 RG PP1T 0050 SP PP1ToH 0050 LC 480 PP1ToH 0048 ABI7500 PP1ToH 0050 RG PP1ToH 0050 SP PPA1T 0050 SP 50 Lactobacilli/Pediococci Lightcyler 2.0 Lightcyler 480 ABI 7300 ABI 7500 RotorGene (FAM, ROX) Biorad (FAM, ROX) First-Wine PCR Screening Detection of lactobacilli and 50 pediococci TLP 0050 LC 2.0 TLP 0050 LC 480 TLP 0050 ABI7300 TLP 0050 ABI 7500 TLP 0050 RG TLP 0050 SP Detection of Lactobacilli, TPWS Pediococci, Oenococcus oeni, Acetic acid bacteria, Yeast universal Bacteria, qualitative (FAM/HEX) with Inhibition-Control (HEX/ROX/CY5) Alicyclobacillus spp. TPAB Alicyclobacillus multiplex and TPABM differentiation of A.spp, A.acidoterrestris, A.acidocaldarius (FAM, HEX, CY5) Acetic acid bacteria TPA Biogenic amine producing bacteria BAM

14 Lactobacillus amylolyticus/ reuteri TaqMan -Differentiation TPLAR Lactobacillus brevis, L.brevisimilis TPLB Lactobacillus TaqMan -Differentiation TLBCL 0048 ABI 48 brevis/ lindneri / casei, paracasei (Fam, Hex, Bodipy) Lactobacillus buchneri, parabuchneri TPLBU Lactobacillus casei, paracasei TaqMan --Detection TPLCP Lactobacillus casei, paracasei, rhamnosus, zeae TPLCR Lactobacillus collinoides/brevis FRET-Detection with Differentiation HPLBC (LC 640) Lactobacillus collinoides/brevis/lindneri FRET-Detection with Differentiation HPLBCL (LC 640, 705) Lactobacillus collinoides, paracollinoides TPLC Lactobacillus coryniformis TPLCO Lactobacillus lindneri TPLL Lactobacillus perolens TPLPER Lactobacillus plantarum, parapl., pentosus TPLP Lactobacillus rossiae TPLR Lactococcus lactis SybrGreen -Detection PLLA Megasphaera cerevisiae TPM Oenococcus oeni TPOe Pectinatus cerevisiiphilus TPPC Pectinatus frisingensis TPPF Pectinatus spp. FRET-Detection HPP 0050 TPP Pectinatus spp. / Megasphaera spp. FRET-Detection with Differentiation HPPM (LC610, 705) Pectinatus spp. / Megasphaera spp. TaqMan - Differentiation TPPM Pediococcus acidilactici SybrGreen -Detection PPA Pediococcus damnosus TPPD Pediococcus pentosaceus SybrGreen -Detection PPP Selenomonas lacticifex Zymomonas mobilis FRET-Detection variable FRET-Detection variable HPSEL HPZYM

15 Pathogene Bacteria, qualitative with Inhibition-Control First-Campylobacter jejuni with internal Control First-Campylobacter jejuni Plus kit: Incl. DNA-extraction kit Campylobacter jejuni Complete kit: ready to use PCR, incl. DNA-extraction kit Campylobacter diff. PCR (jejuni, lari and coli differentiated) with internal Control) Campylobacter diff. Complete: ready to use PCR, incl. DNA-extraction kit Campylobacter PCR (jejuni, lari and coli/ FAM) with internal Control (JOE) Campylobacter Plus kit, incl. DNA-extraction kit Campylobacter Complete kit ready to use PCR, incl. DNA-extraction kit First-Enterobacteria spp. PCR Salmonella enterica PCR with internal Control Salmonella Plus kit, incl. DNA-extraction kit Salmonella Complete kit ready to use, incl. DNA-extraction kit First-Legionella Multiplex PCR Kit Listeria spp. and Listeria monocytogenes multiplex PCR with internal Control Listeria spp. Plus kit, Listeria spp. + L. monocyt. incl. DNA-extraction kit Listeria Complete kit, Listeria spp. + L. monocyt. ready to use PCR, incl. DNA-extraction kit Listeria monocytogenes with internal Control Listeria monocytogenes Plus kit, incl. DNA-extraction kit Listeria monocytogenes Complete kit, ready to use, incl. DNA-extraction kit TaqMan - Differentiation (FAM, JOE, ROX, CY5) TaqMan - Differentiation (FAM, JOE, ROX, CY5) (FAM, JOE) (FAM, JOE) (FAM, JOE) CJE SECJE SECJEC CAMPD SECAMPDC CAMP SECAMP SECAMPC ENT PHS FSDPHS FSDPHSC Real-time PCR-Kit for detection and TPLEGM identification of Legionella spp. and Legionella pneumophila (FAM, HEX, CY5) TaqMan -Differentiation LIST (FAM, JOE, CY5) TaqMan -Differentiation SELIST (FAM, JOE, CY5) TaqMan -Differentiation (FAM, JOE, CY5) SELISTC LMONO SELMONO SELMONOC

16 PCR-Detection-Kits Microorganisms All PCR-Kits are available for different realtime PCR machines on request Yeast Screening with differentiation: Hyb Probe Screening LC 2.0 Hyb Probe Screening LC 480 Multiplex FRET-PCR Detection and Differentiation of Saccharomyces spp., Dekkera spp., Pichia anomala for LC 2.0 (LC640, 610, 705) Multiplex FRET-PCR Detection and Differentiation of Saccharomyces spp., Dekkera spp., Pichia anomala for LC 480 (LC640, 610, 705) Yeast, qualitative (Fam) with Inhibition-Control (Hex/Rox) PYHYB 0050 LC PYHYB 0050 LC Candida spp. FRET-Detection HPYC (Variable) Dekkera spp. FRET-Detection HPYD (LC640, 705) Dekkera anomala TPYDA Dekkera bruxellensis, quantitative TPYDB Pichia anomala TPYPA Pichia membranaefaciens TPYPM Saccharomyces diastaticus TPYSD Saccharomyces exiguus FRET-Detection HPYSX (LC640, 705) Saccharomyces pastorianus/bayanus FRET- Detection with HPYPB Differentiation (LC640, 705) Top-fermented yeast TPYOG Bottom-fermented yeast TPYUG Saccharomyces diastaticus / Top-fermented yeast Saccharomyces diastaticus / Bottom-fermented yeast with Differentiation (FAM, ROX/HEX) TaqMan -Differentiation (FAM, ROX/HEX) TSDOG TSDUG

17 PCR-detection-Kits Animal species All PCR-Kits are available for different Realtime PCR machines on request Realtime PCR, qualitative with internal Control (FAM/HEX) First-Cattle Cattle PHCA First-Chicken Chicken PHC First-Donkey Donkey PHDO First-Duck Duck PHD First-Goat Goat PHG First-Horse Horse PHH First-Meat Mammalia and Poultry PHM First-Pig Pig PHP First-Sheep Sheep PHSP First-Turkey Turkey PHT First-duplex Donkey/Horse Duplex PCR donkey and horse PHDOH First-duplex Cattle/Pig Duplex Beef and Pork PHCAP First-duplex Turkey/Chicken Duplex Turkey and Chicken PHTC Quantification is possible by combination of First-Meat as reference system with any other single specific animal-detection-kit

18 Allergens Realtime PCR, qualitative with internal Control (FAM/HEX) First-Almond Almond PALM First-Cashew Cashew PCAS First-Celery Celery PCEL First-Hazelnut Hazelnut PHAZ First-Lupine Lupine PLUP First-Duplex Mustard Mustard white, brown/ black PMUS (FAM/HEX/Cy5) First-Peanut Peanut PPEA First-Soya Soybean PSOY First-Sesame Sesame PSES Plant species All PCR-Kits are available for different Realtime PCR machines on request Realtime PCR, qualitative with internal Control (FAM/HEX) First-Canola Canola PCAN First-Corn Corn PCOR First-Cotton Cotton PCOT First-Flax Flax PFLAX First-Papaya Papaya PPAP First-Potato Potato PPOT First-Rice Rice PRIC First-Wheat Wheat PWHE First-Plant Plant in general, single copy gene PPLANT First-Plant Plant in general, single copy gene PPLANT

19 Equipment Acrylic-Pipette-Rack Ergonomic pipette rack for 4 pipettes. Suitable for commercial pipettes from µl. Art. No.: PIP4-19 -