Tecan detection portfolio: readers, washers and microarray instruments

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1 Tecan detection portfolio: readers, washers and microarray instruments A portfolio of modular systems built around your application needs

2 Tecan Who we are Tecan is a leading global provider of life science laboratory instruments for the biopharmaceuticals, forensics, clinical diagnostics and academic sectors, specializing in the development and production of automation and detection solutions, including microplate readers, microarray products and washers. Founded in Switzerland in 1980, Tecan has manufacturing, research and development sites in both North America and Europe, and maintains a sales and service network in 52 countries. To date, Tecan has distributed approximately 20,000 microplate readers worldwide, and is committed to continuous technological improvements and compliance with the highest global quality standards. Power Washer 384 SLT 210 Reader Spectra 96PW ProfiBlot Columbus Spectra Fluor Plus Ultra Sunrise 2

3 GENios GENios Pro Infinite 200 Infinite M1000 GCM Safire Safire2 HydroFlex NanoQuant Plate Infinite 200 PRO Infinite M1000 PRO LS Reloaded HS 4800 Pro HS 400 Pro Infinite F500 PowerScanner HydroSpeed Infinite F50 Robotic Infinite F50 3

4 Multimode microplate readers Tecan s user-friendly and high-performance multimode reader, designed for today s applications Infinite F500 Filter Excellent sensitivity for homogenous and cell-based assays in a filter-based reader Detection capabilities/modules/options Filter-based absorbance Fluorescence intensity top & bottom TRF Fluorescence polarization with fast filter switching HTRF Luminescence Automated z-focus for all top reading modes to adapt instrument settings to assay parameters Shaking and temperature control for assay optimization 6- to 384-well plates, including standard, half-area and low volume 384-well plates 1536-well optics for all top readings Barcode reading Up to 2 integrated injectors with 3 different syringe sizes Optional Magellan data analysis software i-control and optional Magellan Integratable into Freedom EVO series Filter-based multimode microplate reader tailored to your applications Infinite F200 PRO Filter Detection capabilities/modules/options Filter-based microplate reader Filter slide with patented system for monitoring of filter lifetime Fluorescence intensity (FI) top reading including TRF and TR-FRET/HTRF FI bottom with OR (optimal read) function for sophisticated cell-based measurements Fluorescence polarization for binding studies Dual-color luminescence assays with integrated filters for BRET1 and BRET2 applications Temperature control, improved shaking and optional injectors for cell-based assays 6- to 384-well plates, low volume plates and patent pending NanoQuant Plate i-control reader software and optional Magellan software Patent pending GCM (Gas Control Module) for rigorous environmental control (CO 2 and O 2 ) within the reader chamber 4

5 Infinite M1000 PRO Monochromator Tecan s most flexible monchromator-based microplate reader for drug discovery and assay development Detection capabilities/modules/options Multi-channel absorbance AlphaScreen /AlphaLISA technology Fluorescence intensity top & bottom Fluorescence intensity (FI) top reading including TRF and TR-FRET/HTRF* Excitation, emission, absorbance, luminescence and 3D scans Flexible wavelength selection from UV to NIR Adjustable bandwidth settings (patented) Fast fluorescence polarization Luminescence: attenuation capability of high light levels Z-focusing in all top reading modes Shaking and temperature control for assay optimization Free plate format definition (6 to 1536-well plates) Injectors with variable syringe sizes Integrated stacker for up to 50 microplates Barcode reading left or right i-control and optional Magellan * The Infinite M1000 PRO is HTRF compatible in white plates Infinite M200 PRO Monochromator Monochromator-based multimode microplate reader for excellent performance & flexibility Detection capabilities/modules/options Monochromator-based absorbance microplate reader, including wavelength scanning Fluorescence intensity (FI) top reading including TRF, automated z-adjustment and background corrected z-focusing FI bottom with OR (optimal read) function for sophisticated cell-based measurements Dual-color luminescence assays with integrated filters for BRET1 and BRET2 applications Temperature control, improved shaking and optional injectors for cell-based assays 6- to 384-well plates, low volume plates, cuvettes and patented NanoQuant Plate i-control reader software and optional Magellan software Patent pending GCM (Gas Control Module) for rigorous environmental control (CO 2 and O 2 ) within the reader chamber 5

6 Absorbance readers Tecan s dedicated absorbance readers for ELISA assays and other basic applications Versatile Sunrise absorbance reader for 96-well plates Sunrise Absorbance reader ELISA, kinetics and agglutination reading Advanced 12-channel optics for fast reading of a plate in only 6 seconds Netbook or touchscreen user interface Selection of eight languages for easy operation Optional: temperature control up to 42 C; gradient filter for wavelength scanning; barcode scanner Advanced curve-fitting & plate-to-plate QC functions IQ / OQ procedure & documentation Designed to meet IVDD 98/79/EC & FDA s 21 CFR part 11* Advanced 3-year warranty Magellan data analysis software * Using Magellan Tracker software Infinite F50 family Absorbance reader Compact ELISA microplate reader with latest LED technology ELISA, kinetic and multilabel measurements Fast 8-channel absorbance microplate reader Long-life LED ensures maintenance-free operation Wizard-based software interface in eight different languages Compact, innovative design that fits into any laboratory Tutorials and example files for getting started easily Full qualitative and quantitative EIA analysis, including all major curve fittings and comprehensive QC function Outstanding reliability proven by unique benchmark value (MTTF**) Together with Magellan Tracker software, designed to meet 98/97/EC IVD-D and FDA 21 CFR Part 11 for use in regulated environments ** mean time to first failure Infinite 200 PRO NanoQuant Filter or Monochromator Upgradeable absorbance plate reader for 2µl sample volumes Basic instrument Filter- or Quad4 Monochromator-based absorbance microplate reader Temperature control Flexibility in plate formats: 6- to 384-well plates, half-area plates, low volume plates, cuvettes and NanoQuant Plate High sensitivity in detection of DNA/RNA and protein quantification Additional labeling efficiency measurements (Monochromator) Simple application oriented i-control software Modules/options Patented NanoQuant Plate Upgradeable with all standard Infinite 200 PRO detection modules 6

7 Microarray products Tecan supports all your microarray detection steps: from hybridization to scanning Automated single- and multi-segment microarray slide hybridization HS 4800 PRO HS 400 PRO The HS 4800 Pro and HS 400 Pro Hybridization Stations represent the very latest technology for automated, reproducible microarray processing on single- and multi-segment slides. Convenient handling of slide, system and software Easily interchangeable single, dual and QuadChamber options No cross-contamination during processing of independent sub-arrays on slide 3-in-1 integrated automation of hybridization, wash and dry Unique ABS (Active Bubble Suppression) system avoids bubble artefacts OSND (on-slide nitrogen drying) avoids external drying step and drying artefacts Protection from ambient light and ozone PowerScanner Top-performance open-format microarray scanner for genomics and proteomics Tecan s PowerScanner is the microarray scanner of choice for high-density DNA microarrays and protein microarrays. Open-format microarray scanner for imaging tissue, protein and nucleic acid microarrays Parallel scanning at 635 nm and 532 nm Image resolution from 2 to 40 µm Broad dynamic range Dynamic autofocus for high scan-field uniformity Compact 48-slide autoloader Intuitive GUI, featuring laser intensity adjustment, auto-gain function and online gain balancing Barcode reader Integrated LaserCheck IQ/OQ function and autocalibration 2 customizable emission filter positions per channel Optional Array-Pro V6.3 automated microarray image analysis PC, Windows Vista 64-bit operating system LS Reloaded The microarray scanner that enables the use of DNA and protein microarrays printed in 96- or 384-well microplates The LS Reloaded is a uniquely versatile microarray scanner, capable of scanning microarrays printed on microplates as well as slides. Up to four lasers and 20 emission filters Two channels for simultaneous scanning with two lasers Three grades of confocal measurements Autofocus with selectable focal plane and capability to scan through glass Autogain for optimization of signal level and broad dynamic range Spatial scan resolution from 6 to 40 μm Adjustable angle of incidence of laser beam for Evanescence Resonance slides and plates LaserCheck assists in verifying and documenting the consistent performance of LS 4-slide adapter for integration with HS 4800 Pro and 400 Pro Hybridization Stations and Connect autoloader Array-Pro Analyzer software offers a powerful solution for image analysis and quantitation. 7

8 Plate washers Plate washers with enhanced capabilities 3-in-1 strip washer for ELISA assays in 96-well format HydroFlex Washer Automated washing of magnetic beads Automated filtration of non-magnetic beads ELISA washing and gentle cell washing enabled by adjustable speed settings for aspiration and dispense, and gentle dropwise dispense mode Liquid Level Detection (LLD) system monitors fill levels in bottles, and warns user when critical level is reached Easy operation via predefined plate settings Designed to meet IVDD 98/79/EC in Elisa configuration and 21 CFR Part 11 via HydroControl software IQ / OQ procedure available Plate washer for cells, beads and ELISAs in 96- and 384-well format HydroSpeed Washer Cell Protection : dropwise dispense speed to avoid cell detachment Anti-Clogging : automated start of rinse and soak of wash head to avoid buffer crystallization Easy X-change wash heads which can be cleaned in an external ultrasonic bath 2 magnets per well for optimized magneticbead recovery Adjustable vacuum level and aspiration position for optimized bead recovery 96 indexing wash head for 96- and 384-well plates, 96 and 384 high speed wash heads 3 optimized magnetic carriers for bead based assays: MBS 384 carrier, smart-2 MBS 96 carrier and MBS 96 carrier Advanced vacuum filtration module for non-magnetic beads Easy-to-use touchscreen interface for full on-board operation or with HydroControl software HydroSpeed can be used with Tecan s Connect stacker for batch processing of 50 plates Designed to meet 21 CFR Part 11 and 98/79/EC IVD-D 8

9 Blot processors Save time and money: reagent saving automated blot processing within closed environment ProfiBlot 48 Blot processor Western blot processor for automated washing and incubation of strip-based assays Western blot processor for automated washing and incubation of strip-based assays Processes up to 48 samples per run in disposable tray within an enclosed environment to minimize user exposure to potentially infectious samples Fast washing of 3 assay strips in parallel for high throughput Color-coded reagent delivery system for up to 7 fluids Reagent saving features reduce dead volumes and running costs Separate collection of toxic and biohazardous waste minimizes waste treatment costs Predefined cleaning protocols for easy maintenance and on-board operation ProfiBlot T48 Blot processor Temperature controlled Southern blot processor and Western blot processor Western blot and southern blot processor for automated washing and incubation of strip-based assays Automated processing of up to 48 samples per run Advanced fluid-based system for active heating and cooling of tray Additional top heating element to avoid evaporation during run Integrated reagent pre-heating and magnetic stirrer system Reagent saving features to minimize dead volumes and running costs Fast washing of 3 assay strips in parallel for high throughput On-board operation and BlotWare software for easy programming Auto-calibration of dispense volumes eliminates tedious manual procedures 9

10 Software What do you expect from Tecan software? i-control Intuitive and workflow-oriented reader control software The Infinite series of Tecan readers (Infinite 200 PRO series, Infinite F500 and Infinite M1000) comes complete with i-control, the easy-to-use microplate reader software. It allows the user to create a workflow for each application by dragging and dropping the processing steps into the sequence required by the assay protocol. The application workflow is then visible to the user and can be saved for future use. Data is easily managed and exported to Windows compatible formats (Excel ). Magellan Get easy access to data analysis in various languages Magellan supports the complete range of Tecan microplate readers, enabling quick and easy measurements of samples. A versatile user interface and powerful data reduction capabilities allow straightforward optimization of sophisticated experimental work, as well as making routine tasks easy. Powerful data analysis software Magellan Standard for research use Magellan Tracker FDA 21 CFR part 11 and 98/79/EC IVD-D compliant: available in 9 different languages MagellanCE allows the on-board operation of the Tecan Sunrise touchscreen reader: available in 9 different languages 10

11 Accessories Tecan s accessories help to make your daily laboratory work more comfortable and your data more reliable Connect Fast, reliable and easy to use batch processing for microplate assays Short and tall cassettes holding 30 and 50 plates respectively Almost any plate type may be used, including all commonly used 96-, 384- and 1536-well plates Optional barcode scanner for either left or right Optional dark cover to protect light-sensitive well contents from laboratory lighting Connect is compact and allows front access to filter slides and plate transport of Infinite 200 readers and Infinite 500 readers, as well as HydroSpeed plate washer GCM (Gas Control Module) for Infinite 200 PRO readers GCM (Gas Control Module) Tecan s new patent pending GCM (Gas Control Module) is a breakthrough for cell-based experiments, offering rigorous environmental control within the detection chamber to minimize inconsistent and unreliable data in cell-based experiments. Simultaneous control of CO 2 (0 10%) & O 2 (0,5 21%) within reader chamber Stabilizes ph and color of media Consistent physiological conditions predictable culture growth Walkaway operation, reducing labor time and enabling higher productivity Lower risk of contamination eliminates tedious transport of cell cultures between incubators and readers for measurement Overnight measurement with complete data sets Fully upgradeable for all Infinite 200 PROs Acoustic & visible warnings avoiding incorrect gas concentrations Altitude correction guarantees precise and stable regulation of gas concentration inside the reader chamber MultiCheck NanoQuant Plate Confidence throughout the whole life cycle Fully automated check of operational performance of all measurement modes Up to 100 instrument parameters checked No sample preparation or pipetting steps necessary Fully automated software routine Tecan s patented NanoQuant Plate: a powerful plate for nucleic acid applications Highly sensitive quartz-based tool for measurement of 16 samples in parallel For 2 µl sample volumes Detection of nucleic acid concentrations, quality control and labeling efficiency Compatible with multichannel pipette for fast loading of samples Patented (EP ) No need for calibration 11

12 R R Overview of all certified applications and regulations Infinite F200 PRO Infinite M200 PRO Infinite F500 Infinite M1000 PRO x x x x T RANSCREENER Red FI validated x x x x T RANSCREENER Far Red FP validated x x LanthaScr ertified d + x x x x x x* Sunrise Infinite F50 HydroFlex HydroSpeed ProfiBlot (T)48 Magellan x x x*** x*** x x** x** x** x**** x**** x** * The Infinite M1000 is HTRF compatible in white plates ** Magellan Tracker software *** ELISA configuration **** HydroControl software Australia Austria Belgium China Denmark France Germany Italy Japan Netherlands Singapore Spain Sweden Switzerland UK USA Other countries Tecan Group Ltd. makes every effort to include accurate and up-to-date information within this publication; however it is possible that omissions or errors might have occurred. Tecan Group Ltd. cannot, therefore, make any representations or warranties, expressed or implied, as to the accuracy or completeness of the information provided in this publication. Changes in this publication can be made at any time without notice. All mentioned trademarks are protected by law. For technical details and detailed procedures of the specifications provided in this document please contact your Tecan representative. This brochure may contain reference to applications and products which are not available in all markets. Please check with your local sales representative. Tecan, Infinite and Freedom EVO are registered trademarks and HydroFlex, HydroSpeed, i-control, Instant Read, Magellan, MultiCheck, LaserCheck, HydroControl, NanoQuant Plate, Anti-Clogging, QuadChamber, Quad4 Monochromators, Premium Quad4 Monochromators, Sunrise, Connect, BlotWare, Cell Protection, HS 400 Pro, HS 4800 Pro, PowerScanner, 96PW, Columbus, LS Reloaded, GENios, Power Washer 384, Safire2 and ProfiBlot are trademarks of Tecan Group Ltd., Männedorf Switzerland. Windows and Excel are registered trademarks of Microsoft Corporation, Redmond, WA, USA. HTRF is a registered trademark of Cisbio international, France. The fluorescence ratio associated with the HTRF readout is a correction method developed by Cisbio international and covered by the US patent 5,527,684 and its foreign equivalents, for which Cisbio international has granted a license to Tecan Group Ltd. Its application is strictly limited to the use of HTRF reagents and technology, excluding any other TR-FRET technologies. Array-Pro Analyzer is a registered trademark of Media Cybernetics, Inc., Silver Spring, MD, USA. Transcreener HTS Assay Platform is a patented technology of BellBrook Labs, LLC USA. Transcreener is a registered trademark of BellBrook Labs, LLC USA. DLReady is a trademark of Promega Corporation, USA. LanthaScreen is a trademarks of Invitrogen Corporation Carlsbad, USA. AlphaScreen and AlphaLISA are registered trademarks by PerkinElmer, USA. 2012, Tecan Trading AG, Switzerland, all rights reserved. For disclaimer and trademarks please visit V2.0, ,