Supplementary Figure 1 Sizing and Relaxometry of Derivatized Ferumoxytol. a, Dynamic light scattering shows no change in diameter between the

Size: px

Start display at page:

Download "Supplementary Figure 1 Sizing and Relaxometry of Derivatized Ferumoxytol. a, Dynamic light scattering shows no change in diameter between the"

Britton York
5 years ago
Views:

1 Supplementary Figure 1 Sizing and Relaxometry of Derivatized Ferumoxytol. a, Dynamic light scattering shows no change in diameter between the clinical specimen (Ferumoxytol) after buffer exchange and the derivatized and radiolabeled 89 Zr-Ferumoxytol. A clinical sample of 99m Tc-Sulfur colloid is included for comparison. b, Likewise, there is no detectable change in the relaxivity (R2) of the two species of superparamagnetic iron oxide nanoparticles. Average values ± standard deviation of triplicate measurements.

2 Supplementary Figure 2 Radiolabeled Nanoparticle Instant Thin Layer Chromatography. Here, the independent activity spectra are displayed, along with a, the ITLC spectra from Figure 1b. b, Free 89 Zr in EDTA running buffer. c, 89 Zr-DFO- Feraheme following the labeling reaction but prior to purification demonstrates the highly efficient labeling. d, Post-purification 89Zr-DFO-Feraheme. A timecourse following purification at e, 10 h; f, 24 h; and g, 48 h.

3 Supplementary Figure 3 89 Zr-Ferumoxytol Biodistribution. Biodistribution of injection site, lymph nodes and major organs following dermal paw injection of 89 Zr-DFO-Ferumoxytol. This graph displays the data contained in Figure 2f with a linear axis (n=4-5, ± SEM). This helps to identify the small amount of material that initially leaks from the injection site into the blood upon injection (liver accumulation) and the presence of 89 Zr-DFO that becomes free from the biodegradable nanoparticle (kidney).

4 Supplementary Figure 4 Full Body and Magnified Magnetic Resonance Imaging Without Nanoparticle Contrast. a, Magnified thoracic coronal MR slice of the area containing the axillary draining nodes in a representative mouse without 89 Zr-Ferumoxytol. Without the use of the nanoparticle contrast agents, it is very difficult to distinguish the presence of the nodes from the surrounding soft-tissue. b, Magnified image of the thoracic area in the coronal plane. c, A magnified MR image of a slice through the same area from the axial perspective. All scale bars represent 0.8 cm.

Supplementary Figure 5 SPECT/CT Imaging of Axial LN Drainage with 99m Tc-radiocolloid. a, 3D-rendered SPECT/CT of axillary node uptake from a paw-dermal injection in the mouse.

5 Supplementary Figure 5 SPECT/CT Imaging of Axial LN Drainage with 99m Tc-radiocolloid. a, 3D-rendered SPECT/CT of axillary node uptake from a paw-dermal injection in the mouse. Despite a higher dose (150 µci) and longer duration of imaging (50 min), there is obfuscating background and signal noise in comparison to the PET/CT and PET/MR approaches with 89 Zr-Ferumoxytol. b, A planar SPECT/CT image demonstrates that while the draining node can indeed be localized (arrow), the specious noise (for example outside of the animal and on the contralateral side) is a considerable issue. Scale bar represents 0.8 cm.

Supplementary Figure 6 SPECT/CT Imaging; Alternate Thresholding Alternate representations of the SPECT/CT fusion data for distinction of the injection site and draining node of an animal administered

6 Supplementary Figure 6 SPECT/CT Imaging; Alternate Thresholding Alternate representations of the SPECT/CT fusion data for distinction of the injection site and draining node of an animal administered 99m Tc-SC in the forepaw. a, Three dimensional reconstruction with full dynamic range of the scan (mimum and maximum presented values). Note that as little of the 99m Tc has migrated, it is difficult to distinguish any site other than the injection site. b, Intensity range min-max of 1-4 % of the total count intensity. Here, identification of the draining node is possible, however as the threshold must be decreased in order for visualization, the noise inherent in SPECT data is more pronounced. c, Finally, using a range of % we are again unable to distinguish the draining node. This range removes the issue of spurious noise blocking visualization of lower intensity foci, however, there is insufficient particle movement to highlight the node.

7 Supplementary Figure 7 Full Body Images Showing Anatomic Localization of the Healthy and Diseased Prostate. a and b, Whole body coronal MR slices that intersect the murine prostate (green arrows point to the ventral prostate; VP). The enlarged prostate of the Hi-Myc mice is indicative of the presence of disease. c and d, Consecutive coronal MR slices of a Hi-Myc mouse injected with the multimodal 89 Zr-Ferumoxtyol, with PET information co-registered and alpha-blended atop the MR images. In the first slice (c) we can see the site of injection, noted with an arrow and asterisk (at the site of the anterior prostate, AP) and a draining lymphatic channel. In the subsequent slice (d), we see the draining LN outside of the prostate organ. e, A schematic of the murine prostate is provided for reference (seminal vesicles, SV; lateral prostate, LP). Scale bars are 0.8 cm in length.

8 Supplementary Figure 8 Coronal MR Scan Parameters. A coronal MR slice of an animal injected in the forepaw with 89 Zr-Ferumoxytol showing hypointensity at the draining lymph node. The scan parameters are burned into the image, defining a resolution of 220 µm in-plane, with a thickness of 800 µm. The resolution of MR imaging can be significantly higher, however even at this resolution, the clear distinction of the contrast for identification of the draining node is possible. Scale bar, in centimeters is present at right.

9 Supplementary Figure 9 Alpha Function for Three Dimensional MRI Reconstruction. The representative MR acquisition rendered into a three dimensional volume (Figure 2) is represented with the alpha transfer function shown here. Here, the intensity of the voxels of the image are mapped to a given opacity, enabling visualization throughout the animal.

Supplementary Figure 10 Resected Tissue LYVE-1 Immunofluorescence. Lyve-1 staining of a slide containing slices of the three resected tissues.

10 Supplementary Figure 10 Resected Tissue LYVE-1 Immunofluorescence. Lyve-1 staining of a slide containing slices of the three resected tissues. a, The first draining node from the prostate, b, and the second are positive for lymphatic endothelium. c, The third is apparently connective and adipose tissue. Scale is 500 µm.

Predictable Heating and Positive MRI Contrast from

Predictable Heating and Positive MRI Contrast from a Mesoporous Silica-Coated Iron Oxide Nanoparticle Katie R. Hurley a, Hattie L. Ring ab, Michael Etheridge cd, Jinjin Zhang be, Zhe Gao ad, Qi Shao c,