ISTA Session at ISSS meeting Alison A Powell Chair Seed Science Working Group

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1 ISTA Session at ISSS meeting Alison A Powell Chair Seed Science Working Group

2 10th Conference of the International Society for Seed Science Seed Science in the 21st Century Costa do Sauípe, State of Bahia, Brazil April, 2011 ISTA / ABRATES Session Francisco Kryzanowski

3 Application of seed science to the evaluation and improvement of seed quality Françoise Corbineau (Université Pierre et Marie Curie, France) Jose Marcio Rocha Faria (Federal University of Lavras, Brazil) Marie-Hélène Wagner (SNES, GEVES, France) Alan Taylor (Cornell University, USA) Stan Matthews (University of Aberdeen, UK)

4 Françoise Corbineau Physiological, biochemical and molecular markers of seed quality Markers of seed quality: Cell deterioration: leakage, ethylene Defense system efficiency: catalase activity, antioxidants The germination process: soluble sugars, cell cycle Related to papers at ISSS meeting Jose Marcio Rocha Faria The use of germinated orthodox seeds in studies on desiccation sensitivity Orthodox seeds as a model for recalcitrant behaviour

5 Marie-Hélène Wagner Knowledge based research towards accurate and rapid testing of seed quality in winter rape Ageing markers: Seed biotinylated proteins Catalase activity Automated germination time course Alan Taylor The interface of seed treatment and coating technologies with seed biology Measuring the water status of coated seeds Aw most consistent Phytotoxicity of seed treatments vermiculite, dry fine peat moss and water at a 1:1:4 ratio Seed coat permeability to systemic compounds Fluorescent tracers

6 Stan Matthews Evaluation of seed quality: from physiology to international standardization Ageing repair hypothesis: basis of vigour differences Evidence: MGT, leakage, ageing tests, cold test etc Related hypothesis to basic physiology International validation of tests within ISTA Positive comments about ISTA / ABRATES session!

7 Other sessions Seed development and maturation: Proteome analysis, functional genomics, NMR imaging of sugars; Arabidopsis, Medicago trunculata Seed dormancy and germination Gene expression, Hormonal and molecular regulation of dormancy Seed and seedling stress tolerance Many omics papers Genome integrity during germination and its repair Non-invasive diagnosis of stress and viability

8 New seed uses and seed biotechnology Modifying expression of thioredoxin in cereals to improve pre-harvest sprouting resistance (Peggy Lemaux,UC Berkeley) Role of gibberellin in determining wheat grain size and quality. (Simon Vaughn, Rothamsted Research, UK) Magnetic Resonance Spectroscopy - Fagus sylvatica (Fabio Gorian) Seed ecology Seed bank persistence; dormancy cycling (Arabidopsis/ molecular ecology); ecological constraints to tropical forest seed analyses Biodiversity and germplasm conservation Seeds and natural capital. The science and economics of conservation (Hugh Pritchard) Sorption/desorption curves in relation to storage potential; Cryopreservation of maize (David Mycock)

9 Brazilian seed networks Amazon: Isolde Ferraz Cerrado Research network: validation of tests for forest species.

10

11 General Comments Work on omics very dominant Complex presentations; rarely related to anything practical Some poor appreciation of basic seed biology / physiology Model species Arabidopsis, Medicago trunculata Significance of seed quality beyond genetic quality not always appreciated Products of innovation presented and marketed no science publications or validation to support them

12 Results reported with potential for development in seed testing Collaboration in place GEVES/UPMC; Aberdeen and Leeds University Collaboration in the future? Potential in basic work not always recognised by ISSS ISTA has expertise and understanding of seed quality that could provide an outlet for basic work Need to work hard at listening to each other

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