Experimental field in Slovenia: Ajdovščina. Fighting algae can be a tiring task. Draining environment. Where this happens in a

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1 The treatment of (fish) ponds on an environmentally friendly manner with the usage of the LG Sonic. Experimental field in Slovenia: Ajdovščina Aquaculture, or aqua farming, is the poisoning or suffocating them. cultivation of aquatic organisms, such as fish or shrimps, in a controlled Fighting algae can be a tiring task. Draining environment. Where this happens in a the water container and filling it with fresh closed water container, algae may occur water only helps for a short period and that pollute the water and may damage might even cause more severe algal the organisms grown. Many of the problems by shifting the algal population to traditional methods to fight algae are faster growing algae. This is caused by either insufficient, cumbersome, having disturbed the previous water environmentally unfriendly, or all of ecology system present. As algae multiply these. at very high rates your clear water can LG Sonic uses ultrasound to control literally turn green overnight. As the algae algal growth efficiently without these are so small that they can pass right negative side effects. through a pond filter, or will be accumulating so intensively that they Green water algae are tiny, single celled (especially filamentous algae) will cause organisms that cause the pond to resemble blockage of your filter, additional methods 'pea soup'. This is the result of a vast are needed. Known algaecides fight algae, population of algae feeding off excess of but will harm the fish and aquatic plants in nutrients (especially phosphorus and the container. The newest ultrasound nitrogen) in your aquaculture container. It techniques provided by LG Sound will help becomes impossible to see deeper then you establish a healthier, cleaner about 2cm from the water surface. environment in your fish pond. In addition, if there is no aeration available and/or there are too much poisonous algae (cyanobacteria or blue green algae) in the water, the health of your fish or other aquatic populations will suffer. Therefore the green water algae can seriously damage the aquatic organisms by LG SOUND

2 The LG Sonic Installation: Experimental ponds. LG SOUND

3 Results part 1: The algae Reference pond LG Sonic treated pond Algae are suspended in the water. Algae are at the bottom of the pond LG SOUND

4 Results part 1: The algae 180,00 160,00 140,00 120,00 O2 (%) 100,00 80,00 A-EXP Poo B-REF Pool O2 % Monitoring 60,00 40,00 20,00 0,00 9:00 12:30 16:00 19:30 23:00 2:30 6:00 9:30 13:00 16:30 20:00 23:30 3:00 6:30 10:00 13:30 17:00 20: :30 Time 16,00 14,00 12,00 10,00 O2 (mg/l) 8,00 A-EXP Poo B-REF Pool O2 mg/l Monitoring 6,00 4,00 2,00 0,00 9:00 12:30 16:00 19:30 23:00 2:30 6:00 9:30 13:00 16:30 20:00 23:30 3:00 6:30 10:00 13:30 17:00 20: :30 Time 25,00 20,00 Temperature 15,00 10,00 5,00 0,00 9:00 12:30 16:00 19:30 23:00 2:30 6:00 9:30 13:00 16:30 20:00 23:30 3:00 6:30 10:00 13:30 17:00 20: :30 A-EXP Poo B-REF Pool Temperature was stable during the test Time LG SOUND

5 Results part 1: The algae Chlororphyll a measurements: LG Sonic treatment free chl. a [mg/m 3 ] bound chl. a [mg/m 3 ] no treatment free chl. a [mg/m 3 ] bound chl. a [mg/m 3 ] ,0 4,5 817, ,0 10,6 4,4 1069, ,9 7,8 16,3 1401, ,0 12,5 1013, ,0 82,4 18,3 210, Bound chlorophyll a in LG Sonic treated pond Bound chlorophyll a in untreated pond Levels of bound chlorophyll a in both ponds Free chlorophyll a in LG Sonic treated pond Free chlorophyll a in untreated pond Levels of free chlorophyll a in both ponds LG SOUND

6 Part 3: Fish monitoring 2007 Basin A LG Sonic Basin B Reference 15th May 29th Oct. 15th May 29th Oct. Weight/fish (kg)þ Total weight (kg)þ Mass growth (%)þ Fish in the LG Sonic treated tank could grow to a higher biomass and grow faster. LG SOUND

7 Part 3: Fish monitoring LG Sonic treated pond: Algae have formed a sediment on the bottom of the pond. Biofilm and algal layers where easy to remove. Untreated pond: Pond lining was covered with biofilm which was hard to remove even after scrubbing the lining. The untreated pond (right) contains a thick layer of biofilm even after cleaning. The LG Sonic treated pond could be cleaned easily. LG SOUND

8 Conclusion: A difference could be seen within 1 week of treatment. The fish in the LG Sonic treated LG Sonic treatment. Algae in the pond are also a little bit heavier and the LG Sonic treated pond ware at the bottom oxygen levels in the LG Sonic treated pond of the fish pond. At the other hand, the water is slightly higher then in the reference pond. that has not been treated is very green. Some Phd.'s in fishery biologist tested the Biofilm. behaviour of the fish (strain: Cyprinus c. Regarding fouling and biofilm formation on carpio Linaeus 1758) in both ponds. surfaces of the two ponds: When cleaning the ponds, the surface of the The results: no significant changes in the pond in which LG Sonic has been used, is fish behaviour were detected. The oxygen much cleaner then the reference pond. level in the pond with LG Sonic treatment The pond in which LG Sonic has been used was slightly higher then the one without is much easier to clean than the reference LG Sonic treatment. pond. Biofilm formation in the LG Sonic pond Algae. seems to be much lower then in the The algae in the pond with the LG Sonic reference pond. The original colour of the device were at the bottom of the pond. They material (black) that has been used to cover were weakened and the water became the bottom of the pond can be seen clearly clear. The algae in the LG Sonic treated in the LG Sonic treated pond. pond die slowly (many were still alive but At the other hand, the colour of the lining in weakened at the bottom of the pond). the referenc pond is now greyish. Chlorophyll molecules are good protected The original colour (thus, black) in the within their cellular body and thus are still reference pond could not be recovered with able to do photosynthesis. Because of the the cleaning method which has been used. relatively shallow (about 0,80 m) and very clear water they will still get enough sunlight. This experiment has been performed in collaboration with: In this way they provide themselves with - University of Portsmouth enough nutritients they synthesize, this might help in their own nourishing, thus - University of catania causing them to die slowly. But, because the algae formed a sediment on the bottom of - Universität für Bodenkultur Wien the pond, they are much easier to be (Boku) removed. The fish in the LG Sonic treated pond can detect their food much easier because of the clear water. Certain stress, the fish normally encounter, in green water fishponds is not available in the pond with LG Sonic LG SOUND Gerrit van der Veenstraat CD Leiden The Netherlands Tel: +31(0) Fax:+31(0) info@lgsonic.com LG SOUND