Microsized Bioreactors for Medical Purposes. Technologies Advancements for Health Rome, Felix Schütze

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1 Microsized Bioreactors for Medical Purposes Technologies Advancements for Health Rome, Felix Schütze

2 Motivation Development of alternatives for animal experiments in research Animal experiments should be avoided, reduced or improved - EU Parliament 2010 In 2014 approximately 2.7 million animals were used for scientific investigations

3 Motivation Personalized medicine animal testings for suitability of pharmaceutical agents

4 Bioreactor of University of Applied Sciences Wildau Microbioreactor for Cultivation of ALN Manufacturing of the Prototype via micro milling machine Individual production during the design study cost-effective manufacture by injection molding PhD Andrea Böhme, Tor Vergata 2016 Feasibilty Study for injection Molding

5 Bioreactor of University of Applied Sciences Wildau Microbioreactor for Cultivation of ALN Manufacturing of the Prototype via micro milling machine Individual production during the design study cost-effective manufacture by injection molding PhD Andrea Böhme, Tor Vergata 2016 Feasibilty Study for injection Molding

6 Micro Injection Molding for Micro Bioreactors New Approach

7 Bioreactor for Injection Molding

8 Bioreactor for Injection Molding

9 Bioreactor for Injection Molding

10 Injection molding inlay

11 Injection molding tool

12 Micro Injection Molding

13 Fluid simulation for the molding process Parameters: closing force Injection temperature injection pressure

14 Microscopic analysis Microscopic image of the reactor chamber at 100x magnification

15 3d-Scans of the microbioreactor Overlay of the CAD-File with the 3D-Scan

16 Joining of the Microbioreactors Production process of microbioreactors Injection molded parts are joined together

17 Functional testing Leak test with cyanobacteria Functional reactor Cells are located within the reactor chamber after flushing

18 Control and regulation system for temperature Aluminiumheat spreader PowerMOSFET NTC- Thermalsensor LABView

19 Experimental setup Proof of functionality and stability of the system Simultaneous tests in cultivational system and CO2-Incubator Test period72h

20 Functional testings with L929 Fibroblasts Cultivational System Conventional System Live cell stain Evaluation under the microscop

21 Glucosis Monitoring Enzymfree detection of glucosis Micro-Electromechanical-systems (MEMS) size 1,3 x 0,36 mm Thesis Liebscher, Tor Vergata

22 ph measurement PhD Rogge, Tor Vergata 2017

23 Thank you for the attention

24 List of Publications Schütze, F.; Böhme, A.; Foitzik, A.H. Manufacturing of a miniaturized biorector for cell culture, ISBN , International Scientific Conference New Ways in Manufacturing Technologies, Schütze, F.; Schneider, S.; Liebcher, T.; Böhme, A.; Foitzik, A.H Miniaturisierter bench top Kultivator mit integrierter Online-Analytik, ISBN , 12. Chemnitzer Fachtagung Mikrosystemtechnik Schütze, Felix; Böhme, Andrea; Santo Loredana; Quadrini, Fabrizio; Foitzik Andreas H. Micro seals from Rubber Hybrid Systems; 13. Chemnitzer Fachtagung Mikrosystemtechnik 25./ Schütze, Felix; Böhme, Andrea; Radke, Lars; Sauer, Sabine; Santo Loredana; Quadrini, Fabrizio; Foitzik Andreas H.; Miniaturised bioreactor system for cultivating in Life Sciences; Technische Systeme für die Lebenswissenschaften; Felix Schütze, Nelson Matuschek, Steffen Zinn, Andrea Böhme, Loredana Santo, Andreas H. Foitzik; Simulation, Manufacturing and Evaluation of Injection Molded Microbioreactor; AMA Conference, Nuremberg, 2017 Felix Schütze, Nelson Matuschek, Steffen Zinn, Loredana Santo, Andreas H.Foitzik; CFD-based optimization of a micro injection molding process; NWK, Mittweida, 2017