Gene expression and function analysis sample and assay technologies by QIAGEN

Transcription

1 Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN s manual and automated workflow solutions for: Sample collection and disruption RNA stabilization and purification Real-time PCR and RT-PCR and gene expression assays RNAi and gene silencing mirna purification and assays Methylation analysis in epigenetics research GEFPortf0309S1WW Protein sample preparation and assays Making improvements in life possible Sample & Assay Technologies

2 From the Science Archives: Apollo 11 On July 20, 1969, Neil Armstrong and Edwin Buzz Aldrin achieved one of the most significant scientific breakthroughs of the 20th century by becoming the first men to walk on the moon. In 1970, Science published a special issue devoted to this remarkable achievement, filled with analyses of the lunar samples the astronauts brought home and experiments they conducted on the moon. In honor of the 40th anniversary of this historic event, Science is opening its online archives, Science Classic, to make this commemorative issue free with registration. To get your free access visit Science Classic online at: For full access to Science Classic, become a AAAS/Science member at AAAS.org/join or recommend Science Classic to your librarian at

3 GE Healthcare Biacore Biacore systems from inspiration...to publication Highest quality, information-rich interaction data from Biacore systems deepen your understanding of molecular mechanisms and interaction pathways and enable you to add function to structure. Select the perfect solution for your application and draw conclusions with confidence from the company that continues to set the standard for label-free protein interaction analysis. For further information or register to have one of our scientific experts contact you, please visit Biacore T100 unmatched performance Biacore X100 ready to run research system Biacore Flexchip array-based comparative profiling 2009 General Electric Company All rights reserved. GE Healthcare Biacore AB, a General Electric Company.

4 Rats the Gene Knockout You Always Wanted ADD. DELETE. SWAP. EDIT FOR THE BETTER. Sigma and Sigma-Aldrich are registered trademarks belonging to Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P. CompoZr is a trademark of Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P. sigma-aldrich.com

5 EXCEPTIONAL QUALITY Restriction Enzymes from New England Biolabs With 35 years of experience in enzyme technology, New England Biolabs leads the industry in quality, selection and innovation for restriction enzymes. Our expertise in purification coupled with a reliance on recombinant technology results in highly pure enzymes suitable for even the most sensitive applications. Additionally, stringent quality controls ensure that our enzymes deliver optimal performance. For quality you can count on, make NEB restriction enzymes your first choice. Power and purity of NEB Restriction Enzymes /N M Incubation time (minutes) Advantages: Selection More specificities than any other supplier Convenience Optimal activity for over 160 enzymes in a single buffer 4 Quality State-of-the-art production and stringent QCs Innovation HF enzymes engineered for reduced star activity Performance Guaranteed Recombinant HindIII is powerful enough to digest 1 µg of DNA in 5 minutes, but can also be used in overnight digests with no indication of nuclease contamination. Marker (M) is the 1 kb DNA Ladder (NEB #N3232). r Choose recombinant enzymes for a higher quality product with lot-to-lot consistency CLONING & MAPPING DNA AMPLIFICATION & PCR RNA ANALYSIS PROTEIN EXPRESSION & ANALYSIS GENE EXPRESSION & CELLULAR ANALYSIS

6 Introducing Stemedia. Culture Stem Cells Stemedia. Rationally Designed. Affordable. Healthy cell cultures are the bedrock of biological research. And Stemgent knows that the hardest cells to maintain properly are stem cells. Your ability to keep your stem cells healthy, in an efficient and affordable manner, requires media that are consistent, reliable, and completely free of contaminants. That s why we ve introduced a new line of Stemedia : the Stemgent way to culture stem cells. Designed from the ground up with components chosen for stem cell utility. Support long-term culture of stem cells. Enhance every step in cell culture. MORE % Q-PCR Q-PCR levels of gene expression %GAPDH Passage 2, Matrigel OCT NANOG FGF NutriStem XF/FF Competitor A NutriStem XF/FF Competitor A Competitor B Proliferation I. hescs (H1, passage 6) were seeded in 96-well plates (Matrigel coated) in the different media. Media were changed every 24 hours. The number of cells was determined using CyQuant cell proliferation assay kit. II. Evaluation of NutriStem hescs (H9.2) using Matrigel as a matrix. Growth promotion for hescs cultured in NutriStem versus competitor A. Cell counts are reported for days 2, 4, and Cells Fold of Change NutriStem XF/FF Day 2-4 Day 4-7 Competitor A Day 2 Day 4 Day 7 The First Stemedia Product Available Now: NutriStem XF/FF Culture Medium A N Morphology hesc (H1) morphology at passage 5 in NutriStem B 10x Teratoma Formation hescs (H1) cultured with NutriStem for 14 passages were injected subcutaneously into SCID mice. Teratomas were harvested after 8 weeks and processed for histological analysis. Tissue types from all three germ layers could be identified in the sections (A C). (A) ectoderm with neural rosette (arrow marked N); (B) endoderm with columnar epithelium (arrow marked E); (C) mesoderm with cartilage (arrow marked C). E C C 20x The first medium in the line, NutriStem XF/FF Culture Medium, is the only xeno-free human embryonic stem cell culture medium on the market. A proprietary, animal component-free, serum-free medium formulation eliminates risk of animal virus contamination. Low bfgf-formulation reduces cost and allows you to better control your cell culture system. NutriStem XF/FF Medium is a complete, readyto-use formulation (containing Alanyl-glutamine). Supports expansion of hes cells in feeder-free culture conditions (on Matrigel ) or on feeder layers (human foreskin fibroblasts, MEFs). MORE MORE Get to the precise information you need faster. When you see one of these link bars, go to Stemgent.com and add the /xxxx extension. It s a direct link to the info you re looking for. Advertisement

7 With New Media From Stemgent. Stemgent Raises the Bar For Human Embryonic Stem Cell Media. Stemgent is committed to producing a complete system of cell culture products, including media, substrates, cytokines, and enzymes. Our scientific advisory board represents a unique association of some of the most accomplished minds in stem cell science. Our work is not only inspired by our board, but everything we produce must maintain their high standards through every step of production, delivery, and support. All Stemedia are rigorously tested by Stemgent and at board members labs. Batch to batch consistency reduces unnecessary variables in your culture. Better performance and lower cost mean a more efficient laboratory for you. SSEA4 expression: hescs (H1) were cultured in different media for 6 passages. OCT4 expression: hescs (H1) were cultured in different media for 6 passages. Gene Expression Analysis by Flow Cytometry In 20% SR medium, on feeders Competitor A Isotype control NutriStem XF/FF Medium Competitor B Autofluorescence Competitor A NutriStem XF/FF Medium Competitor B In 20% SR medium, on feeders Isotype control MORE Our Mission. DAPI Phase SSEA4 OCT4 Immunostaining hescs (H1) show expected morphology and immuno- fluorescence staining of pluripotency markers A B C D Our purpose is to move stem cell science forward. To that end, two things are required. First we need to work with the world class scientists responsible for the breakthroughs that make stem cell research possible in the first place. Second, we need to make that science available in a virtually failsafe way, so that your work benefits from their work. That means that our product offerings are proven.they work.they do what we say they will do. After all, we re not doing experiments, you are. Come to for more information. Or call (toll-free) or (international). Embryoid Body Formation hescs (H9.2) were cultured with NutriStem using Matrigel matrix for 16 passages and tested in vitro for pluripotency by EB formation. After being transferred to suspension culture using serumsupplemented medium, the cells spontaneously formed EBs containing cells representing embryonic germ layers. Histological sections of 14-day old EBs showed the following cell types: (A) neural rosette (ectoderm), (B) neural rosette stained with anti-tubulin, (C) primitive blood vessels (mesoderm) and (D) megakaryocytes (mesoderm). H&E stain. REPROGRAMMING THE REAGENT BOSTON SAN DIEGO Developed and manufactured by Biological Industries Biological Industries Technion R&D Foundation Co-developed in collaboration with the Technion R&D Foundation 2009 by Stemgent, Inc. Stemgent, Reprogramming the Reagent, the What s Next logotype, Stemedia and NutriStem are trademarks of Stemgent, Inc. Matrigel is a trademark of BD Biosciences. CyQuant is a trademark of Molecular Probes. All rights reserved. Advertisement

8 I know you ll like what we came up with. Get your best GEICO rate on auto insurance as an AAAS member. Visit geico.com for your FREE, no-obligation rate quote. Be sure to select AAAS when asked for your affiliation. New customers report an average savings of $500 when they switch. Go to geico.com, contact your local office, or call Some discounts, coverages, payment plans, and features are not available in all states or in all GEICO companies. Discount amount varies in some states. Discount is not available in all states or in all GEICO companies. One group discount applicable per policy. In New York a premium reduction is available. AAAS is compensated for allowing GEICO to offer this auto insurance program to AAAS members. GEICO auto insurance is not available in Mass. Government Employees Insurance Co. GEICO General Insurance Co. GEICO Indemnity Co. GEICO Casualty Co. These companies are subsidiaries of Berkshire Hathaway Inc. GEICO: Washington, DC GEICO Geckoimage GEICO Advertisement High Performance Antibodies For High Credibility Characterizations. Stemgent Small Molecules. Essential. Science Signaling A new journal from the publishers of Sciencee Recent reports have shown that replacement of transcription factors with small molecules is capable of improving the efficiency and kinetics of ips cell generation. Furthermore, addition of small molecules to the culture media can help generate more homogenous self-renewing ips cell populations. Sheng Ding, Ph.D. Associate Professor of Chemistry, Scripps Research Institute Member, Stemgent Advisory Board The breakthrough work of Stemgent Advisory Board member Dr. Sheng Ding continues to provide new insight into the importance of small molecules in stem cell research. Stemgent now offers 23 small molecule products all proven to have epigenetic effects on ips or ES cells. As our research continues, new products will be added. Work with Stemgent to take full advantage of small molecules in your research. REPROGRAMMING THE REAGENT Science Signaling offers science researchers the most up-to-date resource for groundbreaking research and commentary in the dynamic field of cellular signaling. Contact Information: Toll free International customers, call or sciencesignaling@aaas.org ISSN: Set up a FREE 90-day trial for your institution today! subscriptions/ inst_trialform.dtl BOSTON SAN DIEGO 2009 by Stemgent, Inc. Stemgent, Reprogramming the Reagent and the What s Next logotype are trademarks of Stemgent, Inc. All rights reserved.

9 LIFE SCIENCE TECHNOLOGIES New Products AAAS/Science Business Office Microfluidics Chip Functionality Dolomite now offers the ability to integrate metal electrodes into its range of glass and polymer microfluidic chips. A wide variety of geometries can be achieved, such as interdigitated electrodes, heaters, and temperature sensors. Applications include impedance detection, temperature control in microreactors, and generation of electrical fields for dielectrophoresis. With its excellent chemical-resistant and heat-resistant properties, platinum is most frequently used as an electrode material, but a wide range of metals can be deposited onto the microfluidic glass or polymer chips, including gold, copper, titanium, and chrome. Metal feature size can be as small as 2 µm. Metal layers can also be deposited on the inside surface of the microchannels. Dolomite For information Desktop Nanofabrication The next generation Dip Pen Nanolithography (DPN) system, the DPN 5000, brings greater control and performance to desktop nanofabrication. The DPN 5000 offers versatile nanopatterning capabilities coupled with high-performance atomic force microscopy imaging for immediate characterization of the deposited patterns. System highlights include an ultralow-noise scanner with closed-loop flexure technology to produce accurate and repeatable nanoscale patterns. For subsequent imaging of substrates, a low-coherence laser with a reduced laser spot size ensures high-quality lateral-force imaging. Enhanced lithography software, InkCAD 4.0, includes improved control of tip-based patterning, along with nanoscale mapping and positioning, allowing users to precisely position multiple features, even those created from different materials. NanoInk For information Sperm Freezing Kit The JAX Sperm Cryo Kit provides a reliable and cost-effective method of cryopreserving the sperm of novel mouse models. Researchers can preserve the sperm in their own laboratories, while still enjoying the benefit of having The Jackson Laboratory perform quality control testing and provide long-term storage. The kit contains all the necessary items, including collection dishes, straws and cassettes, freezing apparatus, cryo-protective media, and a detailed instruction manual. It even includes extra reagents and plasticware to perform a practice run. The Jackson Laboratory will perform a quality control test on one sperm sample from each strain to assess fertility and report the results back. The Jackson Laboratory For information Confocal FRAP Imaging The VT-HAWK combines high precision point-scanning fluorescence recovery after photobleaching (FRAP) with multibeam confocal imaging. The instrument s ability to switch between imaging and photo-bleach modes in seconds enables accurate quantitation of fluorescence intensity dynamics in precise, user-defined regions of the image. VT-HAWK supports FRAP, fluorescence loss in photobleaching, photo-activation, and photo-conversion techniques with selectable pinhole sizes for confocal imaging. The instrument s optics deliver optimal laser beam quality at the sample without compromising the performance of either mode. Options include a lasermerge module that houses up to six solid-state lasers. VisiTech International For information +44-(0) Metabolite Database The Symyx Metabolite database, a comprehensive collection of drug metabolism data, is now available to pharmaceutical, agrochemical, and biotechnology researchers via the Symyx Isentris data access, analysis, and decision support system, as well as the web-based DiscoveryGate content platform. The database can accelerate research and development productivity by making it possible for scientists to focus on the most therapeutically promising compounds (those with the right metabolic profile) early in the discovery process. Scientists can use Symyx Metabolite s structure-searchable metabolic schemes, biotransformation explorer, and easy-to-use query and browse tools to explore the fate of parent compounds, their metabolites, and properties by leveraging in-house and Symyx database information. Symyx Technologies For information Electrophoresis Software Spresso is free, open-source software for electrophoresis that can ease experimental design and troubleshooting. The software provides a new tool for designing and optimizing complex electrophoresis problems. The designers say that the software is 75 times faster than current tools thanks to unique numerical algorithms. The speed can mean the difference between waiting overnight to get a result and getting it in a few minutes. Spresso allows scientists to iterate through experimental variations much faster than they could by going into the lab and relying on trial and error. Stanford School of Engineering For information microfluidics.stanford.edu/spresso/2008/09/introducing-spresso. html Electronically submit your new product description or product literature information! Go to for more information. Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier JULY 2009 VOL 325 SCIENCE

10 Submission deadline August 1 It s truly exhilarating to be recognized for your hard work. This prize will motivate young generations of scientists and encourage new discoveries. Dr. Christine Jacobs-Wagner Grand Prize winner 1997 The GE & Science Prize for Young Life Scientists. Rewarding brilliance since Imagine standing on the podium at the Grand Hotel in Stockholm, making your acceptance speech. Imagine joining the ranks of those published in Science magazine and having your essay on your work in molecular biology read by your peers around the world. Imagine taking part in a seminar with the other Prize winners and Nobel Prize laureates and discussing your work with leaders in the field. Imagine what you could do with the US$25,000 prize money. Imagine this brilliant start to your career and where it could lead you. Now stop imagining, and make it a reality. Dr. Christine Jacobs-Wagner did just that. In 1997, she entered in the GE & Science PrizeforYoungLifeScientistsandwonthegrandprize.Sowhynotyou?Ifyouareone ofthosewhowereawardedaph.din2008,thensubmityour1000-wordessayby August1,2009.Yourbrilliantideacouldwellbeonitswaytobuildingabetterreality, for you and for those around you. All the information you need awaits you at: imagination at work For the purpose of this prize, molecular biology is defined as that part of biology which attempts to interpret biological events in terms of the physicochemical properties of molecules in a cell. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th Edition). GE Healthcare Bio-Sciences AB, a General Electric Company. Björkgatan 30, Uppsala, Sweden General Electric Company All rights reserved.

11 Everyday needs. Everyday answers. Easily find your favorite brands of reagents, kits, and benchtop devices at Ambion Dynal Gateway GIBCO Lipofectamine RNA and RNA sample prep Magnetic beads for separation Cloning and protein expression Cell culture Transfection reagents Molecular Probes Novex SuperScript TaqMan TOPO Fluorescent dyes and probes Protein separation and blotting cdna synthesis reagents Real-time PCR PCR cloning For research use only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. TaqMan is a registered trademark of Roche Molecular Systems, Inc. These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen catalog or our website, By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. A