ULTIMATE PRE-PACKED PREPARATIVE COLUMN GUARANTEED! NEW PHASES! THE FOR HPLC AND SFC. Axia PREP LC columns offer:
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1 THE ULTIMATE PRE-PACKED PREPARATIVE CLUMN FR HPLC AND SFC GUARANTEED! Axia PREP LC columns offer: Increased Performance Groundbreaking Lifetimes ptimized Loadability Increased Reproducibility NEW PHASES!
2 The Ultimate PREP Column The Axia Advantage Available in over 4 unique achiral and chiral selectivities, Axia advanced preparative column packings and column hardware designs offer several advantages. Unlike traditional column packing methods, the Axia packing method offers increased sorbent bed density for increased performance and eliminates media bed collapse as a source of premature column failure in preparative HPLC/SFC columns. If Axia packed columns do not provide at least an equivalent separation as compared to a competing preparative column of the same particle size, same phase, and dimensions, return the product with comparative data within 45 days for a FULL REFUND. nly applies to. mm ID columns. 7, Inc. All rights reserved. USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
3 Axia Technology...pp. 4-7 Award winning column packing technology Media Selection Chart...pp. 8-9 Quickly find the media for your purification needs Media Selectivity Details...pp. -7 Achiral Chemistries Core-Shell Media NEW Kinetex...pp. -3 Aeris...pp. 4-5 Fully Porous Media Gemini...pp. 6-7 Synergi...pp. 8-9 Luna...pp. - NEW Luna mega...pp. -3 Jupiter...p. 4 Clarity...p. 5 Chiral Chemistries NEW Lux...pp. 6-9 Table of Contents PhenoLogix...pp. 3-3 Screening, ptimization and Scale-up Services SecurityGuard PREP...pp PREP HPLC/SFC Column Protection rdering Information...pp I find Axia columns to be very robust and durable. I often use the prep column for much longer than predicted with reproducible peaks. This saves us a significant amount of money. David Wisnoski GlaxoSmithKline, USA USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 3
4 Axia Technology TRADITINAL PREP PACKING Axia Technology vs. Traditional BD Prep Column Packing Axia Packing Technology Axia packed preparative columns involve a single axial compression step unlike conventional packed preparative columns. The ideal column bed density is custom calculated and automated for each specific media and column size. Computer control of the entire process ensures both proper bed density and column uniformity every time. During the Axia packing process, the packing piston is locked in place, eliminating any decompression and then recompression of the media sorbent, thus maintaining media and column bed integrity. This solves common lifetime and performance problems associated with conventional packing processes for preparative columns. Axia Packing Process Involves: Compression Final Column Media packed under ideal pressure The packing piston head is integrated into the column and locked by the piston retainers, so the pressure is never released. Media is never allowed to relax, eliminating voids and dramatically improving reproducibility, column-to-column. U.S. Patent No. 7, 674, 383 AXIA TECHNLGY Traditional Slurry Packing Traditional slurry packing processes, like the Waters BD (ptimum Bed Density) column packing approach, involve the column being removed from the column packing station once it is packed. Several potential problems with this packing method are: Variability in column performance due to increased number of manual operations required for assembly Potential silica media damage during recompression Level of process control is based on traditional slurry packing technology Conventional Packing Process Involves: Compression Decompression Recompression Final Column Pump Column Media escapes from disconnected column Removal of escaped media Media density disruption and potential media crushing Silica media packed under liquid pressure Violent recompression of silica media Diagram from Waters Corporation U.S. Patent No. 7,399,4 Comparative separations may not be representative of all applications. 4 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
5 Axia packed columns produce uniform media bed with intact particles The highly tuned patented process and hardware eliminates potential decompression ensuring bed stability and optimal packing density. The media found on the inlet frit of the Axia packed column shows no signs of damage unlike the media found on inlet frit of traditionally packed prep columns. Traditional packed preparative columns produce non-uniform media beds with sheared and crushed particles *SEM of Axia inlet frit Intact media at frit surface after packing *SEM of Waters BD inlet frit Axia Technology Decompression and then recompression during packing can damage the media and lead to increased column-to-column variability, flow disturbances, and decreased column lifetimes. *The images are believed to be representative, but individual columns may vary. Crushed media or silica fines at frit surface after packing We are using chromatography media from for GPL/GMP purposes, therefore we audited USA as a manufacturer. From the beginning, we were impressed with and the attitude of their employees. is a unique company in many aspects. Their degree of dedication to customer service, to the organization of the QMS system and last but not least the positive atmosphere in the company is impressive. The outcome of the audit was to our fullest satisfaction. Major Generic Pharma Company, Europe is not affiliated with Waters Corporation. Comparative separations may not be representative of all applications. View an animated packing process comparison at USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 5
6 Axia Technology Axia Technology utperforms Traditional Packing Processes! Because of the constant pressure placed on the integrated packing piston, Axia packed columns possess the dynamic capability of maintaining a consistent, homogeneous media bed. This results in superior column performance no matter which media selectivity you choose. To better understand how much Axia technology improves column performance over traditionally slurry packed preparative columns we scaled-up a 5 μm Lux Cellulose- chiral media analytical column and packed the same media into two different Warfarin Chiral Purification in Normal Phase Mode Analytical mau x. mm I.D. columns. ne column was packed using Axia technology and the other prep column was packed using the traditional slurry packing process. The Axia packing technology had a substantial increase in column efficiency resulting in increased resolution over traditionally packed preparative columns. With increased resolution you are able to increase your sample load enabling you to purify more target compound(s) per purification run. This equates to better throughput and economics. Column: Lux 5 µm Cellulose- Dimensions: 5 x 4.6 mm Mobile Phase: Hexane/Ethanol (75:5) Flow Rate: ml/ min Temperature: Ambient Inj. Volume: µl App ID min Warfarin Standard Packing and Hardware mv Rs =.85 Axia Technology and Hardware mv Rs = % Increase in Resolution App ID App ID min Conditions for both PREP columns: Media: Lux 5 µm Cellulose- Dimensions: 5 x. mm Mobile Phase: Hexane / Ethanol (75:5) min Flow Rate: ml/ min Temperature: Ambient Inj. Volume: ml Column (mm) Analytical 5 x 4.6 Standard 5 x. Axia 5 x. Mass Loaded (mg) 4 4 Resolution * Plates (N) % Increase in Efficiency * Resolution calculated with peak width at baseline and center retention time due to the overloaded peaks being off-scale Tip: For more detailed information on this warfarin application, view application at: 6 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
7 Unmatched Column Reproducibility The completely automated Axia packing system provides feedback control and infinite tuning of packing density for specific media characteristics such as mechanical strength and porosity. An optimum higher bed density can be consistently reproduced column-to-column. This directly translates into consistent efficiency and peak asymmetry measurements and decreases the column variability seen in traditionally packed preparative columns. Efficiency (N),, 8, Reproducible Column-to- Column Efficiency.8 % RSD.5 7 % Improved Avg. Efficiency Peak Asymmetry.4.3. Reproducible Column-to- Column Peak Asymmetry 9. % RSD 3 % Improved Avg. Peak Shape 5. Density (%) Density Comparison of Packed Beds 9 % Increase in Packing, Density, and More Uniformly Packed Bed Axia Technology 6, , Traditional Slurry Packing Axia Packed Hydraulic Piston Compression Packing Traditional Slurry Packing Axia Packed Hydraulic Piston Compression Packing 53 Traditional Slurry Packing Axia Packed Hydraulic Piston Compression Packing Average Efficiency (N) with Synergi 4 µm Hydro-RP x. mm Average Peak Asymmetry with Gemini 5 µm C8 5 x. mm Axia columns provide me with first rate quality and engineering. Reliability, reproducibility, and durability are provided with all Axia columns that I use. I can literally purify 5 samples per column. The time and cost savings are tremendous. Derrick Miyao Large Biotech Manufacturer, USA We have used Axia prep-hplc columns for several years and they consistently provide excellent separation and reproducibility for a variety of different compounds. Jeremy R. Wolf ABC Laboratories, USA USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 7
8 Media Selection Phase Selection Chart Quickly find the media for your purification needs. yes Basic Compound no Small Molecule Achiral Compound Molecule Size Biomolecule yes Aromatic Compound no Gemini C6-Pheny or Gemini NX-C8 Gemini NX-C8 or Gemini C8 Kinetex Biphenyl or Phenyl-Hexyl Kinetex EV C8 (ph -) or XB-C8 (ph <8) Luna mega PS C8 Luna NH (ph <) yes Aromatic Compound no Kinetex F5, Biphenyl, or Phenyl-Hexyl yes Polar Compound no Synergi Polar-RP Luna Phenyl-Hexyl or PFP() Kinetex F5 or HILIC Luna mega Polar C8 or PS C8 Kinetex EV C8, XB-C8, C8, or C8 Gemini NX-C8 Luna Si(), HILIC, PFP(), or CN Luna C8() or C8() Synergi Polar-RP, Hydro-RP, or Fusion-RP Synergi Max-RP Gemini C8 8 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
9 ligo Molecule Type Peptide/Protein Chiral Compound Lux Cellulose-, Cellulose-, Cellulose-3, Cellulose-4, Amylose-, Amylose-, or i-cellulose-5 Media Selection Clarity ligo-xt, ligo-rp, or ligo-wax <, Da Molecular Weight >, Da Luna NH (ph <) Aeris XB-C8 Jupiter 3 Å C8 or C4 Luna mega Polar C8 or PS C8 Kinetex EV C8, Biphenyl, XB-C8, or C8 Luna C8, Phenyl-Hexyl, C8, C5, or SCX Jupiter Proteo Kinetex st Core-Shell Preparative Column Ever! Pages -3 Luna Proven Purification Performance Pages - Luna mega High Efficiency Polar and Non-Polar Purifications Pages -3 Aeris Core-Shell Peptide Media Pages 4-5 Synergi Unique Chemistries for Complex Mixtures Pages 8-9 Jupiter Increase Loadability for Biomolecule Separations Page 4 Clarity Purification of Synthetic ligonucleotides Page 5 Lux Polysaccharide Supports with Excellent Enantioselectivity Pages 6-9 Gemini High ph Separations Pages 6-7 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 9
10 Kinetex Media First Core-Shell Preparative HPLC/SFC Column Ever! Kinetex Core-Shell Technology produces increased efficiencies over traditional, fully porous columns, yielding remarkable chromatographic resolution, higher peak capacities, and greater sensitivity, so labs can get even more out of their HPLC analyses! The benefits of Kinetex Core-Shell Technology include: Increased efficiencies over traditional fully porous columns Seamless scalability from HPLC/UHPLC to Preparative LC Kinetex 5 µm provides better performance than traditional fully porous 5 and 3 µm materials.3 µm.7 µm.6 µm 5 µm High Column Efficiency Combining 5 μm Kinetex core-shell and Axia technologies can provide the highest separation efficiency of any pre-packed preparative HPLC column. Waters XBridge 5 µm C8 Prep BD Kinetex 5 µm XB-C8 Axia Packed Response - Millivolts x 9. mm N = 77, plates/meter App ID 456 Réponse- Millivolts x. mm N =,6 plates/meter ver 55 % Increase in Efficiency App ID min min Conditions for both columns: Columns: Kinetex 5 μm XB-C8 Axia Packed Waters XBridge 5 μm C8 Prep BD Dimensions: 5 x. mm (Kinetex) 5 x 9 mm (XBridge) Mobile Phase: Water/Acetonitrile (5:5) Injection Volume: μl Flow Rate: 5 ml/min Temperature: Ambient Detection: 54 nm Sample:. Uracil. Acetophenone 3. Toluene 4. Naphthalene Key: Best Suited Very Good Packing Material Kinetex C8 Kinetex XB-C8 Particle Size (µm).3,.7,.6, 5.7,.6, 3.5, 5 Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range.5-8.5*.5-8.5* Kinetex EV C8.7,.6, 5 - Applications Type of Compounds Loading Small Molecules Peptides Proteins Chiral ligonucleotides Acids Polar Hydrophobic Bases Available Surface Area Kinetex C8.7,.6, * Kinetex Phenyl-Hexyl.7,.6, * Kinetex Biphenyl.7,.6, * Kinetex HILIC.7,.6, Kinetex F5.7,.6, 5 9 % *ph stability under gradient conditions. ph stability is.5-. under isocratic conditions. Comparative separations may not be representative of all applications. USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
11 Excellent Loadability! With narrower peak widths than fully porous columns across every sample load, Axia packed Kinetex 5 µm columns give you the capability of increased sample load and higher throughput for vastly improved purification performance and economics. Waters XBridge 5 µm C8 Prep BD mv min Conditions for both columns: Columns: Kinetex 5 μm C8 Axia Packed XBridge 5 µm C8 Prep BD Dimensions: 5 x. mm (Kinetex) 5 x 9 mm (XBridge) Mobile Phase: A: Water with.5 % Formic acid B: Acetonitrile with.5 % Formic acid Gradient: Time (min) 8 % B 5 5 App ID 775 Kinetex 5 µm C8 Axia Packed mv Increased Sensitivity and Excellent Loadability! min Flow Rate: 3 ml/min Temperature: Ambient Detection: 54 nm Sample: mg/ml in DMS. Doxepin (From - 5 mg on-column). Amitriptyline (From - 5 mg on-column) Core-Shell Technology App ID 774 Kinetex Media Kinetex Axia Preparative columns are fantastic! I currently use two Kinetex 5 µm C8 5 x. mm columns in parallel for high throughput purifications (< mg scale), and Kinetex core-shell media delivers significantly improved peak shape and lower back pressure compared to many of the industry. I can also analyze quickly my purified fractions with the same core-shell phase on my analytical UPLC system. Chris DeVore Neurocrine Biosciences, USA Tip: If you would like to see a loading study performed with the combination of Axia Packing, view application at: Comparative separations may not be representative of all applications. USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
12 Kinetex Media Seamless Scalability from HPLC/UHPLC to PREP Kinetex packed with Axia technology makes it the first coreshell sorbent commercially available for small-scale preparative applications. Combine this with the fact that the entire Kinetex mau Waters XBridge 5 µm C8 5 x 4.6 mm Impurity Not Resolved core-shell line is fully scalable from.3 µm to 5 µm, this means that transferring high performance HPLC/UHPLC methods to preparative HPLC and SFC formats is fast and simple. mau Kinetex 5 µm EV C8 5 x 4.6 mm Excellent Resolution App ID 74 App ID min min Analytical to PREP Scalability Conditions for all columns: Columns: Kinetex 5 µm EV C8 XBridge 5 µm C8 Dimensions: 5 x 4.6 mm 5 x. mm (Kinetex AXIA Packed) Mobile Phase: A:. % TFA in Water B:. % TFA in Acetonitrile Gradient: % to 7 % B over minutes Flow Rate:.5 ml/min 3 ml/min (Kinetex AXIA) Temperature: Ambient Detection: 54 nm Sample: Proprietary Pharmaceutical Sample Kinetex 5 µm EV C8 5 x. mm AXIA App ID min My Axia packed column has a great efficiency for the separation of several classes of natural compounds. Due to its low back pressure and therefore high flow work conditions, time for conditioning the columns is sped up greatly! Sylvian Cretton -Europe Comparative separations may not be representative of all applications. Tip: For more information on the power of Kinetex core-shell scalability, view application at: USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
13 A Broad Spectrum of Column Selectivities Kinetex core-shell columns are available in a wide range of stationary phases, allowing you to optimize your separation for maximum resolution and loadability across HPLC, UHPLC, and preparative HPLC and SFC applications. mau C A B C D C8 E F G H App ID 84 min mau XB-C A B D C Si XB- C8 E F G H App ID 84 min Kinetex Media EV C8 C8 mau 8 mau A B C + D E F G EV C8 H App ID A B C D C8 F E G H App ID min min Biphenyl Phenyl-Hexyl mau mau A B Biphenyl 4 C D E F G H App ID 845 min 6 4 A B C D E Phenyl- Hexyl F G H App ID 847 min Kinetex 5 µm F5 F F F F F F5 NEW Conditions for all columns: Columns: Kinetex 5 µm C8 Kinetex 5 µm XB-C8 Kinetex 5 µm EV C8 Kinetex 5 µm C8 Kinetex 5 µm Biphenyl Kinetex 5 µm Phenyl-Hexyl Dimensions: x 4.6 mm Mobile Phase: A:. % TFA in Water B:. % TFA in Acetonitrile Gradient: Time (min) % B Flow Rate:.5 ml/min Temperature: C Detection: 33 nm Sample:. Chlorogenic Acid thers: Antioxidants from green coffee Tip: For more information on Chlorogenic Acids from Green Coffee by HPLC, view application at: rdering information on page 34 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 3
14 Aeris Media Increased Performance for Peptide Purifications Based on core-shell particle technology, Aeris PEPTIDE media is designed with small pores ( Å), an inert XB-C8 surface chemistry, and multiple particle sizes to meet the selectivity, resolution and loading demands of chemists working with synthetic peptides. The benefits of Aeris PEPTIDE columns include: ptimized media for peptide purifications Multiple particle size options for method development flexibility and peptide impurity analysis Seamless scalability from HPLC/UHPLC to preparative HPLC Si XB- C8 XB-C8 chemistry best suited for resolving peptides Multiple Particle Sizes For Added Versatility PREP UHPLC Analytical.5 µm Solid Core. µm Porous Shell.7 µm.35 µm Porous Shell.9 µm Solid Core.6 µm.6 µm Solid Core 3.6 µm.5 µm Porous Shell 3.8 µm Solid Core 5 µm.6 µm Porous Shell solid core solid core.7--- solid core solid core Key: Best Suited Very Good Packing Material Aeris PEPTIDE Particle Size (µm).7,.6, 3.6, 5 Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range.5-9. Applications Type of Compounds Loading Small Molecules Peptides Proteins Chiral ligonucleotides Acids Polar Hydrophobic Bases Available Surface Area 4 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
15 Develop, Purify, and Analyze Peptide Fractions with ne Media Aeris PEPTIDE is fully scalable in retention and selectivity with its 4 unique particle sizes (.7 µm,.6 µm, 3.6 µm, and 5 µm) for easy transfer from HPLC and UHPLC methods to preparative applications. Aeris PEPTIDE.6 μm XB-C8 mau 8 Bio Core-Shell Technology Analytical method Column: Aeris PEPTIDE.6 µm XB-C8 Dimensions: 5 x 4.6 mm Part No.: F-455-E Injection Volume: µl Flow Rate: ml/ min Sample: Crude peptide mix Aeris Media 6 4 App ID min Aeris PEPTIDE 5 μm XB-C8 79 mv FR FR FR 3 FR 4 App ID 79 Preparative scale-up and fraction collection Column: Aeris PEPTIDE 5 µm XB-C8 Axia Packed Dimensions: 5 x. mm Part No.: F-463-P-AX Injection Volume: ml Flow Rate: ml/ min Sample: Crude peptide mix min Aeris PEPTIDE.6 μm XB-C8 Analytical fraction analysis Column: Aeris PEPTIDE.6 µm XB-C8 Dimensions: 5 x 4.6 mm Part No.: F-455-E Injection Volume: µl Flow Rate: ml/ min Sample: Purified Fractions Fraction 4 Fraction 3 Fraction Fraction = 97.3 % Purity App ID 78 Conditions for all separations (except as noted): Mobile Phase: A:. % TFA in Water B:. % TFA in Acetonitrile Gradient: Linear 85:5 (A/B) to 5:95 (A/B) over minutes Temperature: Ambient Detection: nm Fraction min rdering information on page 34 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 5
16 Gemini Media Setting the Standard for ph Method Development Gemini features a ph stability from -, making it optimal for high alkaline washes and high ph purifications of basic drugs. ptimized parameters include: Innovative surface layer for increased ph stability High-surface area for increased loading Silica smoothness for stable packing beds Bonding density for excellent reproducibility Second-Generation TWIN-NX Technology Gemini NX-C8 TWIN-NX technology uses an improved, patented organo-silica grafting process which incorporates highly stabilizing ethane cross-linking. These organic groups are evenly incorporated into the grafted layers on the silica surface while maintaining a pure silica core. This not only provides resistance to high ph attack, but also maintains the high efficiency and mechanical strength of a silica particle. ph Flexible LC Si CH CH Si Si S CH CH Si CH CH Si Si Si CH CH CH C CH Si Si CH Si Si CH CH CH CHSi Si Si Si CH CH C C CH CH Si Si Si Si CH CH CH CH CH Si Si Si Si CH CH *This bonding technology is also available in Core-Shell media. See Kinetex EV on page. Dramatically improve sample resolution, productivity and performance of any preparative column media with Axia column hardware and packing technology. Axia packed prep columns offer the opportunity for longer lifetime, higher loading and increased throughput. Gemini 5 µm NX-C8 Axia Packed 5 45 Waters XBridge 5 µm C8 Prep BD % increase in resolution App ID App ID min min Conditions for both columns: Column: Gemini 5 µm NX-C8 Waters 5 µm XBridge Dimensions: 5 x. mm (Gemini) 5 x 9 mm (XBridge) Mobile Phase: A: mm Ammonium bicarbonate ph. B: Acetonitrile Gradient: Time (min) % B Flow Rate: 5 ml/min Temperature: C Detection: 68 nm Sample:. Reserpine. Unknown Key: Best Suited Very Good Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range Gemini C8 3, 5, Small Molecules Peptides Proteins Chiral Applications Type of Compounds Loading ligonucleotides Acids Polar Hydrophobic Bases Available Surface Area Gemini C6-Phenyl 3, Gemini NX-C8 3, 5, Comparative separations may not be representative of all applications. 6 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
17 Flexibility in ph Adjustments Allows for Increased Purification Performance Separating basic compounds at higher ph levels produces dramatic changes when compared to low ph conditions. At ph.5, the basic compounds become neutralized and are more hydrophobic. The retention for the uncharged basic compounds increases providing an increase in separation along with superior peak shapes. Gemini NX-C8 with.5 % TFA Low ph.4 mg in 4 µl App ID 7693 Gemini NX-C8 with. % NH 4 H High ph.5 Excellent resolution at high ph.4 mg in 4 µl App ID 7699 Gemini Media min min Column: Gemini NX-C8 5 μm Dimensions: 5 x. mm Mobile Phase: A:.5 % TFA in Water B: Acetonitrile Gradient: 5 % B to 95 % B in 5 min Flow Rate: 3 ml/min Detection: 54 nm Sample:. Diphenhydramine. Propranolol Column: Gemini NX-C8 5 μm Dimensions: 5 x. mm Mobile Phase: A:. % NH 4 H in Water B: Acetonitrile Gradient: 5 % B to 95 % B in 5 min Flow Rate: 3 ml/min Detection: 54 nm Sample:. Diphenhydramine. Propranolol Separation shape improvement provides opportunity for increased loading Gemini NX-C8 with.5 % TFA Gemini NX-C8 with. % NH 4 H Low ph 3 App ID 776 High ph.5 3App ID mg in 4 µl 53 mg in 4 µl min Column: Gemini NX-C8 5 μm Dimensions: 5 x. mm Mobile Phase: A:.5 % TFA in Water B: Acetonitrile Gradient: 5 % B to 95 % B in 5 min Flow Rate: 3 ml/min Detection: 54 nm Sample:. Diphenhydramine. xybutynin 3. Terfenadine min Column: Gemini NX-C8 5 μm Dimensions: 5 x. mm Mobile Phase: A:. % NH 4 H in Water B: Acetonitrile Gradient: 5 % B to 95 % B in 5 min Flow Rate: 3 ml/min Detection: 54 nm Sample:. Diphenhydramine. xybutynin 3. Terfenadine ur Gemini and Luna Axia packed columns are the workhorses in our lab. These columns exhibit outstanding performance for challenging separations while also handling a high workload for standard separations. Longevity has also been excellent with some columns lasting years or more. Dependability is so important in my line of work and these columns never disappoint!! -Major Pharmaceutical Company, USA rdering information on page 34 Tip: If you want longer Gemini NX-C8 Axia packed column lifetimes, view a lifetime study application at: USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 7
18 Synergi Media Increased Loading with Unique Selectivities Synergi is available in four unique phases, each offering dramatic differences in: Selectivity Retention time Resolution The unique selectivity profiles found within the Synergi product line offer complementary selectivity to the standard C8, C8, or silica phases traditionally employed in preparative HPLC. Full Range Selectivity LC Columns Synergi Polar-RP For Polar and Aromatic Mixtures ( % Aqueous Stable) Synergi Fusion-RP Balanced Non-polar and Polar Performance ( % Aqueous Stable) Ether linkage increases aromaticity of the phenyl group and also provides π-π interactions with conjugated compounds Polar endcapping provides added retention for polar compounds Embedded polar group complements C8 ligand with balanced polar selectivity endcapping ensures sharp peaks Aq Aq Aq Aq Phenyl Ether-Linked C8 Polar Embedded Synergi Hydro-RP Strong Non-polar and Polar Retention ( % Aqueous Stable) Polar endcapping provides added retention for polar compounds Synergi Max-RP Excellent for Basic Compounds at Neutral ph High density ligands and extensive endcapping ensure sharp peaks Aq Aq C8 Polar Endcapped C Endcapped Applications Type of Compounds Loading Key: Best Suited Very Good Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range Small Molecules Peptides Proteins Chiral ligonucleotides Acids Polar Hydrophobic Bases Available Surface Area Synergi Fusion-RP 4, * Synergi Max-RP 4, * Synergi Hydro-RP 4, Synergi Polar-RP 4, *ph stability under gradient conditions. ph stability is.5-. under isocratic conditions. 8 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
19 Selectivity Like No ther ffering a balanced combination of hydrophobic and polar selectivity, Synergi Fusion-RP separates compounds exhibiting moderately polar and hydrophobic characteristics. Hydrophobic basic compounds Longer Polar Retention " Synergi 4 µm Fusion-RP 3 Typical C Conditions for all columns: Columns: Synergi 4 µm Fusion-RP Typical C8 Dimensions: 5 x 4.6 mm Mobile Phase: mm Potassium Phosphate, ph.5 / Acetonitrile (75:5) Flow Rate:. ml/ min Detection: nm Sample:. Maleic acid. Chlorpheniramine 3. Triprolidine 4. Diphenhydramine 4 Improved Peak Shape and Superior Selectivity! Less Hydrophobic Retention 4 App ID 484 min min The slightest variations in compound polarity and aromaticity are exploited by Synergi Polar-RP to achieve the greatest separation between polar and/or aromatic compounds. Increased resolution of polar compounds with Synergi Polar-RP compared to traditional C8 phases Improved Selectivity! Synergi 4 µm Polar-RP Longer Polar Retention Waters 5 µm SymmetryShield RPC8 Waters 5 µm XTerra RP8 Waters 5 µm Symmetry C8 6 6 App ID 57 App ID 56 App ID 55 App ID 485 Synergi Media min Conditions for all columns: Columns: Synergi 4 µm Polar-RP Waters 5 µm SymmetryShield RPC8 Waters 5 µm Symmetry C8 Waters 5 µm XTerra RP8 Dimensions: 5 x 4.6 mm Mobile Phase: mm Potassium phosphate ph 3 / Methanol (5:5) Flow Rate:. ml/ min Detection: 3 nm Temperature: Ambient Injection: μl Sample:. Metaproterenol (.4 μg). Pindolol (.6 μg) 3. Metoprolol (.5 μg) 4. Alprenolol (.3 μg) 5. Propranolol (.4 μg) 6. Ethylparaben (.4 μg) We regularly use RP stationary phases from for our separation problems. Especially Synergi Polar-RP which was found to often show the desired selectivity, distinguishing this phase from other RP phases. CARBGEN AMCIS, Switzerland Comparative separations may not be representative of all applications. rdering information on page 35 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 9
20 Luna Media Media for ne of the World s Leading PREP HPLC Columns Luna high surface area (4 m /g) silica packing materials provide optimized parameters specifically designed for the purification of small molecules and peptides. This media allows high loading with excellent lifetimes. ptimized loading parameters include: Silica smoothness for stable packed beds ptimum pore size/distribution provide outstanding performance High pore volume offers increased surface area Fine tuned bonding density for excellent reproducibility Greater loading capacity with an extended ph range of.5 to.* We routinely use Axia packed columns from for peptide purifications. Among various preparative HPLC columns we have used, the Axia packed Luna columns (5 µm) stand out. We have been very satisfied with the increased loading capacity and excellent performance. Guangcheng Jiang Ferring Research Institute, Inc., USA Applications Type of Compounds Loading Key: Best Suited Very Good Packing Material Luna C8() Luna C8() Particle Size (µm) 3, 5,, -PREP, 5 3, 5,, -PREP, 5 Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range * * Small Molecules Peptides Proteins Chiral ligonucleotides Acids Polar Hydrophobic Bases Available Surface Area Luna C5 5, * Luna Phenyl-Hexyl Luna Silica() 3, 5,, -PREP, 5 3, 5,, -PREP, * Luna CN 3, 5, Luna NH 3, 5, Luna SCX 5, 4-7 Luna HILIC 3, Luna PFP() 5, *ph stability under gradient conditions. ph stability is.5-. under isocratic conditions. USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
21 Simple Scale-Up Axia column technology provides the same high efficiency chromatographic performance for preparative scale columns (., 3, and 5 mm ID) as obtained in 4.6 mm ID analytical columns. This improvement in preparative column performance across Absorbance 4 Analytical 5 x 4.6 mm Sample Load:. mg in µl DMS Flow Rate:.5 ml/min App ID 87 all lengths and internal diameters makes it easier to select the appropriate column size to achieve the desired purity and yield without having to compromise on performance. Absorbance 5 Axia PREP 5 x. mm Sample Load:. mg in µl DMS Flow Rate: 3 ml/min App ID 88 Luna Media min min Absorbance 5 Axia PREP 5 x 3 mm Sample Load: 4. mg in µl DMS Flow Rate: 6 ml/min min Columns: Luna 5 μm C8() Dimensions: As Noted Mobile Phase: A:.5 % TFA in Water B:.5 % TFA in Acetonitrile Gradient: A/B (95:5) to A/B (5:95) in 5 minutes App ID 89 Absorbance 5 Axia PREP 5 x 5 mm Sample Load:.5 mg in µl DMS Flow Rate: 5 ml/min min Flow Rate: As Noted Detection: 54 nm Temperature: Ambient Injection: See chromatograms Sample: Suzuki Reaction Mixture App ID 83 Proven Media for Peptide Purifications An optimal compromise between throughput, recovery, and yield. Perform high loading (.74 g on column) and achieve high purity (>98 %) in a single purification run. Preparative Purification of Bivalirudin ( amino acid peptide also known as Angiomax ) Purification Elution Profile at.5 % Specific Load mau mau min Column: Luna µm-prep C8() Dimensions: 5 x. mm Part No.: G-433-P-AX Mobile Phase: A: mm Ammonium acetate ph 4.7 in Water B: Acetonitrile Gradient: to 5 % B in 4 min; hold at 8 % B for 5 min; reequilibration at % B for min Flow Rate: ml/min Temperature: Ambient Detection: 8 nm Injection Volume: 5 ml Sample Concentration: 7 mg/ml in water Sample: Crude Bivalirudin in Water App ID 8 Purity Confirmation of Combined Fractions Combined fractions min; Recovery 8.5 % with purity 98.5 % Peak No. Time (min) Area Area % Column: Luna 5 μm C8() Dimensions: 5 x 4.6 mm Part No.: G-449-E Mobile Phase: A:. % TFA in Water B:. % TFA in Acetonitrile Gradient: % to 5 % B in 3 min min Flow Rate: ml/min Temperature: 5 C Detection: nm Injection Volume: µl Sample: Combined Fractions 4 App ID 79 rdering information on page 35 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
22 Luna Media Luna mega NEW Cutting Edge Fully Porous Silica Particle Luna is one of the most recognized HPLC brands on the market, delivering high efficiency, ruggedness, reproducibility, and dependability for a wide range of analyses. The new Luna mega builds upon this legacy with an innovative yet rugged silica particle architecture, designed and manufactured by based on more than years of applied knowledge, invention, and customer experience. Novel Design and Manufacturing Process Within the novel manufacturing process of Luna mega silica, we implement a proprietary processing technique to gain greater particle inertness, a stronger particle morphology, and more consistent porosity. Micropores Thermal Modified Pore Structure Most importantly, through our proprietary process, we eliminate micropores, further improving column efficiency, inertness, and reproducibility. Consistent Porosity Absence of Micropores 5µm 3 µm.6 µm Applications Type of Compounds Loading NEW NEW Key: Best Suited Very Good Surface Packing Particle Pore Area Carbon ph Material Size (µm) Size (Å) (m /g) Load (%) Range Luna mega Polar C8.6, 3, %.5-8.5* Luna mega PS C8.6, 3, %.5-8.5* *ph stability under gradient conditions. ph stability is.5- under isocratic conditions. Small Molecules Peptides Proteins Chiral ligonucleotides Acids Polar Hydrophobic Bases Available Surface Area USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
23 UHPLC to HPLC to PREP Scalability With direct selectivity scalability from Luna mega.6 µm to 5 µm you can fluidly transfer methods from UHPLC platforms to HPLC and preparative instrumentation. Additionally, you can easily go in reverse and use a Luna mega.6 µm to analyze fractions taken from a Luna mega 5 µm preparative column. Luna mega Phase Selection Polar Polar C8 Polar % aqueous stability and enhanced selectivity/retention for polar analytes without diminishing useful non-polar retention. The C8 ligand provides general hydrophobic interactions while a polar modified particle surface provides enhanced polar compound retention. + PS C8 + MEGA Unique, % aqueous stable mixedmode phase that provides both polar and non-polar retention. The surface contains a positive charged ligand which aids in the retention of acidic compounds through ionic interactions, while the C8 ligand promotes general reversed phase retention. The positively charged surface also improves basic compound peaks shape through ionic repulsion. Luna Media Direct Scalability.6 µm to 5 µm Intensity, cps mau Luna mega.6 μm Polar C8 Luna mega 5 μm Polar C min App ID 3745 Conditions for all columns: Columns: Luna mega.6 μm Polar C8 Luna mega 5 µm Polar C8 Dimension: 5 x. mm Mobile Phase: A: Water with. % Formic Acid B: Acetonitrile with. % Formic Acid Gradient: Time (min) 5 % B 5 95 Flow Rate:.4 ml/min Temperature: 3 C Detection: 54 nm Sample:. Uracil. Pindolol 3. Chlorpheniramine 4. Nortriptyline 5. 3-Methyl-4-nitrobenzoic acid 6. 5-Methyl salicylaldehyde 7. Hexanophenone UHPLC to HPLC to PREP UHPLC Scout Dimension: Flow Rate: App ID min 5 x. mm. ml/min ptimized HPLC mau App ID Dimension: 5 x 4.6 mm Flow Rate:.5 ml/min Preparative Purification mau Dimension: 5 x. mm Flow Rate: 3 ml/min App ID 3757 Conditions for all columns (as noted): Columns: Luna mega 5 μm PS C8 Mobile Phase: A: Water with. % TFA B: Acetonitrile with. % TFA Gradient: Time (min) 5 % B 9 Temperature: C Detection: 54 nm Sample:. Impurity. Proprietary API 3. Impurity Analyze Prep Fractions via UHPLC rdering information on page 35 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 3
24 Jupiter Media Media for Biomolecules The Jupiter HPLC column portfolio, including Jupiter 3 and Jupiter Proteo, offers optimized reversed phase solutions for peptide and protein purification. Identify, purify, and analyze almost any protein with Jupiter columns. Jupiter Proteo 9 Å For separation of proteins and peptides <, MW C bonded onto an ultra-high surface area (475 m /g) silica for increased peak capacity and resolution of peptide separations Direct scale-up from analytical to preparative and bulk materials Resolve Peptides with Similar Hydrophobicity Jupiter Proteo is able to fully resolve peptides that differ in hydrophobicity by one methyl group Jupiter 4 µm Proteo 9 Å Agilent Technologies ZRBAX 5 µm SB-C8 3 Å Alltech Associates, Inc. Vydac 5 µm MS54 3 Å Alltech Associates, Inc. Vydac 5 µm TP54 3 Å min App ID 448 App ID 447 App ID 446 App ID 449 Jupiter 3 Å For separation of proteins >, MW Available with C8 and C4 bonded phases Protein and Peptide LC.5 ph stability for method ruggedness and easy protein removal Direct scale up to preparative and bulk materials Compare PEGylated vs. Native Forms of Proteins Reversed phase separation of PEGylated and native proteins on a Jupiter 3 C4 column. Note the good resolution of multiple PEGylated forms for all proteins tested. mau 8 4 β-lactoglobin A PEGylated β-lactoglobin A Carbonic Anhydrase II PEGylated Carbonic Anhydrase II Insulin PEGylated Insulin 5 min Columns: Jupiter 3 5 μm C4 3 A Dimensions: 5 x 4.6 mm Part No.: F-467-E Mobile Phase: A: % Acetonitrile /. % TFA in Water B: 7 % Acetonitrile / % IPA /.8 % TFA in Water Gradient: A/B (85:5) to A/B (3:7) in 5 min Flow Rate: ml/min Temperature: 45 C Detection: 4 nm Sample: PEGylated and Native Proteins App ID 69 Columns: Jupiter 4 μm Proteo 9 A Agilent Technologies ZRBAX 5 μm SB-C8 3 Å Alltech Associates, Inc. Vydac 5 µm MS54 3 Å Alltech Associates, Inc. Vydac 5 μm TP54 3 Å Dimensions: 5 x 4.6 mm Mobile Phase: A:. % TFA in Water B:.85 % TFA in Acetonitrile Gradient: A/B (95:5) to A/B (55:45) in minutes Flow Rate: ml/min Temperature: C Detection: 4 nm Sample:. NH -Arg-Gly-Gly-Ala-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide. Ac-Arg-Gly-Gly-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide 3. Ac-Arg-Gly-Ala-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide 4. Ac-Arg-Gly-Val-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide 5. Ac-Arg-Gly-Val-Val-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide We purchased the Jupiter 3 C8 3 Å column a few months ago and have been quite impressed with its performance. The Jupiter 3 column provides better separation of the proteins. As for reproducibility, the control profiles have not changed since day one of its use. Major Biotech Company, Europe Key: Best Suited Very Good Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range Jupiter C8 5,, Jupiter C4 5,, Jupiter Proteo 4, Applications Type of Compounds Loading Neutrals Small Molecules Peptides Proteins Chiral ligonucleotides Acids Polar Hydrophobic Bases Available Surface Area Comparative separations may not be representative of all applications. 4 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
25 Purification of Synthetic ligonucleotides Clarity ligo-rp Unique media specifically designed for reversed phase purification of oligonucleotides with balanced hydrophobicity and polar selectivity. The media is based on composite particle TWIN technology that provides improved selectivity and efficiency for oligonucleotides when compared to competing hybrid, polymer, and silica media. RP-HPLC Preparative Purification Easily separate N- failure sequences from target oligo with > 9 % purities Purify oligos up to 6 nt in length Trityl-off purification of DNA, RNA, thioates, and modified/ labeled oligonucleotides 3 μm, 5 μm, μm particles for seamless scaling Preparative nt DNA ligo-rp Purification mau Column: Clarity 3 μm ligo-rp C8 Dimensions: 5 x mm Part No.: B-444-N Mobile Phase: A: 5 mm TEAA ph 7.5/ 5 % Acetonitrile B: Methanol Gradient: % to 6 % B in minutes Flow Rate: 5 ml /min Detection: 6 nm Sample: nt DNA App ID 5947 min Clarity ligo-xt NEW Clarity ligo-xt, C8 columns have been designed to provide rugged high performance for the LC/MS characterization of synthetic DNA and RNA samples, alongside purification of these targeted oligos. With high efficiency levels from the novel core-shell particle design, this new media provides the necessary separation power to accurately resolve closely related oligo sequences. Novel core-shell particle technology with rugged ph stability from - 5 µm particles provide extremely low pressure HPLC and Preparative purification solutions Seamless scalability between all three particle sizes (.7 µm,.6 µm and 5 µm) Clarity ligo-wax Clarity ligo-wax is a crosslinked weak anion-exchange media designed for successful ion-exchange purification of synthetic DNA/RNA. ligo-wax is an advantageous combination of purity, capacity, mechanical strength, cost, and efficiency. Excellent efficiency column results in > 9 % purities due to good fractionation of closely eluting compounds High loading capacity due to very high density ligand Increase productivity by running at higher flow rates and pressures Clarity Media We have used the Axia prep columns and have not had problems with them. I have never had to adjust for retention gaps. This speaks directly to the quality of s phases and the quality of their PREP columns. -Major Biotech Company, USA Applications Type of Compounds Loading Neutrals Key: Best Suited Very Good Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range Small Molecules Peptides Proteins Chiral ligonucleotides Acids Polar Hydrophobic Bases Available Surface Area ligo-rp 3, 5, NEW ligo-wax ligo-xt.7,.6, 5 - USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 5
26 Lux Media Complete Chiral Solutions Achieving optimal chiral separation is easier than ever with seven unique Lux polysaccharide stationary phases to screen. Choose a phase, then transfer the method to lab scale, process, pilot, and commercial scale. Lux chiral preparative columns simplify the separation process: Unique and traditional phases that increase the success rate of the chiral screen Mechanically strong media for increased stability Available in multiple particle sizes for direct scale up (3 μm and 5 μm packed columns for screening and small scale purifications; μm and μm bulk media for process scale purifications) Chiral LC Columns Resolve Your Enantiomers with Seven Unique Phases The Lux family of bulk cellulose and amylose chiral selectors provides a variety of complementary selectivities. NEW CI Cellulose CI NH Lux i-cellulose-5 Cellulose tris(3,5-dichlorophenylcarbamate) Guaranteed Alternative to CHIRALPAK IC and IC-3 H 3 C CH 3 NH Cellulose--CNH Lux Cellulose- Cellulose tris(3,5-dimethylphenylcarbamate) Guaranteed Alternative to CHIRALCEL D, D-H, D-3, D- RH, and D-3R CI H 3 C NH Cellulose Lux Cellulose- Cellulose tris(3-chloro-4-methylphenylcarbamate) Guaranteed Alternative to CHIRALCEL Z, Z-H, Z-3, Z- RH, and Z-3R CH 3 CH 3 Cellulose Lux Cellulose-3 Cellulose tris(4-methylbenzoate) Guaranteed Alternative to CHIRALCEL J, J-H, J-3, J- RH, and J-3R CI NH Cellulose Lux Cellulose-4 Cellulose tris(4-chloro-3-methylphenylcarbamate) Guaranteed Alternative to CHIRALCEL X-H, X-3, X-RH, and X-3R H 3 C CH 3 NH Amylose Lux Amylose- Amylose tris(3,5-dimethylphenylcarbamate) Guaranteed Alternative to CHIRALPAK AD, AD-H, AD-3, AD- RH, and AD-3R H 3 C NH Amylose CI Lux Amylose- Amylose tris(5-chloro--methylphenylcarbamate) Guaranteed Alternative to CHIRALPAK AY, AY-H, AY-3, AY-RH, and AY-3R NEW Key: Best Suited Very Good Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range Lux i-cellulose-5 3, 5, Lux Cellulose- 3, 5,,, Consistent particle size distribution so performance is maintained Chiral Applications Type of Chiral Compounds Loading Small Molecules Peptides Proteins Chiral ligonucleotides Acids Polar Hydrophobic Bases Available Surface Area Lux Cellulose- 3, 5,,, Lux Cellulose-3 3, 5,,, Lux Cellulose-4 3, 5,,, Lux Amylose- 3, 5, Lux Amylose- 3, 5, USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
27 Column Screening for ptimal Chiral Resolution Being able to utilize differences in selectivity in each of the seven Lux columns can help develop methods more efficiently by offering broad and complementary chiral recognition abilities. Etozolin Based on a five phase screen under reversed phase conditions, the optimal chiral stationary phase for resolving Etozolin is Lux Cellulose-3. Lux 5 µm Cellulose-3 a =.7 ptimal Resolution App ID 95 In the example below, a simple screen determined which column gave the best separation. Conditions for all columns: Column: As noted Dimension: 5 x 4.6 mm Mobile Phase: Acetonitrile / mm Ammonium bicarbonate with. % Diethylamine (6:4) Flow Rate: ml/min Temperature: Ambient Detection: nm N S N Lux Media min Lux 5 µm Amylose- a = min Lux 5 µm Cellulose- a =.6 App ID 946 App ID 947 Lux 5 µm Cellulose- a =.48 Lux 3 µm Cellulose-4 a = min App ID 95 App ID min min Innovative chiral selector will succeed where others fail Lux 5 µm Cellulose-4 a =.7 4 CHIRALCEL 5 µm D-H ptimal Resolution App ID min Conditions for all columns: Dimensions: 5 x 4.6 mm Mobile Phase:. % Diethylamine in Hexane /. % Diethylamine in Isopropanol (9:) Flow Rate: ml/min Detection: nm Temperature: Ambient H 3 C CHIRALPAK 5 µm AD -H CH 3 Tolperisone N a =. a =. App ID 954 App ID min min Columns used for comparison were manufactured by DAICEL Corporation. is in no way affiliated with DAICEL Corporation. Comparative separations may not be representative of all applications. USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 7
28 Lux Media Load More with an Increase in Column Length Axia column technology allows separations to scale up directly based on column length. With the mm length column a 3 mg/load separation was achieved and an increased sample α =.9 App ID 778 load of 8 mg/load was achieved on the longer 5 mm length column. As expected when increasing the load, the peak width and tailing increased but there was no loss of resolution. Conditions for all columns: Columns: Lux 5 µm Cellulose- Dimensions: as noted Mobile Phase: Methanol / Isopropanol (9:) Flow Rate: as noted Detection: as noted Sample: Dissolved in mobile phase as noted Dimensions: x 4.6 mm Flow Rate: ml/min Detection: nm Sample: 5 µg in µl H N min H Methocarbamol No resolution loss with increased sample load α =.8 App ID 778 Dimensions: x. mm Flow Rate: ml/min Detection: nm and 54 nm Sample: 3 mg in 64 µl min.5x Load Increase α =.8 App ID 778 Dimensions: 5 x. mm Flow Rate: ml/min Detection: nm and 54 nm Sample: 8 mg in 6 µl min Lux Axia preparative columns are wonderful! I regularly use Lux chiral stationary phases Cellulose- and Cellulose-4 and less frequently, the Lux Amylose-. In our community of chiral analysis/ purification scientists, there are some who use the CC4 column instead of the *equivalent* Lux Cellulose-4. n several occasions we ve seen separation and good peak shape on the Lux Cellulose-4 that was completely missing from the CC4. Customer support and delivery times are always within a few days. Julia G. Christie GlaxoSmithKline, USA 8 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
29 Easy SFC Scale-up SFC Purfication of Terfenadine Baseline Separation of Enantiomers UV-VIS 5 5 min Polarimeter Lux Cellulose- offers great peak shape at nm App ID 8865 Dimensions: 5 x 4.6 mm Flow Rate:.5 ml/min Detection: nm Load: 3 µg in µl Analytical and Axia packed columns have been extensively tested on various SFC systems and all column ID s and lengths are SFC compatible. H N Terfenadine H H C(CH 3 ) 3 Lux Media UV-VIS 5x Load Increase verloading study with increased analytical load showing impurities eluting after major enantiomers only detected at 54 nm min Polarimeter App ID 8866 Dimensions: 5 x 4.6 mm Flow Rate:.5 ml/min Detection: 54 nm Load:.5 mg in 5 µl Conditions for all columns: Columns: Lux 5 µm Cellulose- Mobile Phase: Methanol with. % DEA/ Carbon Dioxide (5:75) Column Temperature: 35 C Polarimeter: ALP-PDR-Chiral Sample: Terfenadine with ethanol dissolution solvent 7x Load Increase 7.5 cycles/hr 787 mg/hr High loading capacity media along with stacking injections allow for increased yields and productivity Closer stacked injections can not be used due to the impurities eluting after the major enantiomers App ID 8867 Dimensions: 5 x. mm Flow Rate: 5 ml/min Detection: nm Load: 5 mg in 3.5 ml Tip: For SFC column screening, use Lux 5 x 3. mm ID columns. rdering information on page 35 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 9
30 A New Era of Technical Support Services Let Us Do the Work for You PhenoLogix, our in-house application support lab, saves you time and money by screening multiple scout columns and solvent strategies for new purification methods or revalidating your current methods. We work together to make you successful by minimizing your process purification development time and optimizing your purification method. Chiral Screening Normal Phase Reversed Phase Polar rganic SFC Method ptimization Services Fast Turnaround Easy Method Transfer Continued Support Preparative and Process Scale-Up Media Screening Small Scale Purification DAC Packing Assistance 3 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
31 Your Method, ur Scientists Quality Products, Advanced Performance, Complete Support For more information or to begin a project today, please contact your local representative or us at phenologix@phenomenex.com You can also visit us online: ur scientists at American Peptide have taken advantage of s column packing services, application development, and project-specific consultation services for some of our most challenging separations. American Peptide Company, USA USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 3
32 SecurityGuard PREP PREP HPLC/SFC Column Protection SecurityGuard PREP Extends preparative column lifetime by as much as 5 times Protects columns from samples that precipitate out of solution Protects columns from contaminants Stable and leak-free up to 6 ml/min within specified pressure ratings The SecurityGuard PREP system was designed to effectively and inexpensively protect your valuable prep columns from the damaging effects of mobile phase and sample chemical contaminants and particulates, without altering your chromatographic results. Lower Your Cost Per Injection! SecurityGuard isn t only about column protection, it s about lowering your cost per injection! When you increase the number of injections from a single preparative column you re lowering your overall cost per injection. With SecurityGuard PREP, the inexpensive cartridge was ruined while the integrity of the prep column was maintained and its performance restored. Forced Degradation Study Injection : Axia Packed column with SecurityGuard PREP cartridge min Column: Luna µm C8() Axia Packed Dimension: 5 x. mm Part No.: B-453-P-AX Mobile Phase: A:. % TFA in Water B:. % TFA in Water / Acetonitrile (5:75) Gradient: Linear 93:7 (A/B) to % B over 5 minutes Injection Volume: 4 µl Flow Rate: 6 ml/min Temperature: Ambient Detection: 7 nm Sample:. Nadolol. Metoprolol 3. Propranolol Injection 4: Axia Packed column with SecurityGuard PREP cartridge 4 3 Time to change the SecurityGuard PREP cartridge min Injection 4: Axia Packed column after removing SecurityGuard column protection system min riginal column performance maintained by using SecurityGuard PREP SecurityGuard PREP PREP HPLC Column 3 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
33 rdering Information SecurityGuard PREP Semi-Prep Preparative (HPLC or SFC) If your column ID (mm) is: Use cartridges (mm): Material Description ph Stability. x. 5. x. 5. x 3. Cartridges for General Purpose/Pharmaceutical $ 53 / 3 pk $ 39 / ea $ 4 / ea C8 (DS, ctadecyl).5 - AJ-7 AJ-7839 AJ-83 C (Dodecyl).5 - AJ-775 AJ-784 AJ-834 C8 (MS, ctyl).5 - AJ-7 AJ-784 AJ-83 C5 (Pentyl).5 - AJ-737 Silica AJ-73 AJ-79 AJ-83 HILIC (HILIC).5-8 AJ-89 NH (Amino, Aminopropyl).5 - AJ-7364 AJ-86 AJ-839 CN (Cyano, Cyanopropyl) AJ-733 AJ-8 AJ-83 Phenyl (Phenylhexyl).5 - AJ-734 AJ-784 AJ-833 PFP() (Pentafluorophenyl).5-8 AJ-8376 AJ-8377 AJ-8378 SCX (SA, Strong Cation Exchanger) AJ-8595 AJ-8596 SAX (SB, Strong Anion Exchanger) AJ-737 RP- (Reversed Phase - Polymer) - 4 AJ-7368 AJ-8358 Polar-RP (Ether-linked Phenyl).5-7 AJ-776 AJ-7845 AJ-837 Fusion-RP (C8 Polar Embedded).5 - AJ-7558 AJ-7844 AJ-836 AQ C8 (Polar Endcapped C8) AJ-75 AJ-7843 AJ-835 Gemini NX-C8 (C8 TWIN-NX Technology) - AJ-8369 AJ-837 AJ-837 Gemini C8 (C8 TWIN Technology) - AJ-7598 AJ-7846 AJ-838 Gemini C6-Phenyl (C6-Phenyl TWIN Technology) - AJ-956 AJ-957 AJ-958 Luna mega Polar C8 (Polar Function C8).5 - AJ-763 AJ-764 Luna mega PS C8 (Mixed Mode C8).5 - AJ-768 AJ-769 Cartridges for Core-Shell PREP Columns $ 53 / 3 pk $ 39 / ea $ 4 / ea For core-shell media columns, such as Kinetex and Aeris (). EV C8 (DS, ctadecyl) - AJ-936 AJ-934 AJ-935 C8 (DS, ctadecyl) AJ-978 AJ-945 AJ-94 C8 (MS, ctyl) AJ-95 AJ-97 PFP (Pentafluorophenyl).5-9 AJ-946 AJ-999 Phenyl-Hexyl (Phenylhexyl) AJ-947 AJ-96 Biphenyl (Biphenyl) AJ-98 AJ-97 AJ-973 HILIC (HILIC) AJ-977 C8-Peptide (DS, ctadecyl).5-9 AJ-937 AJ-938 AJ-939 F5 (Pentafluorophenyl) AJ-933 AJ-934 AJ-935 Cartridges for Protein and Polypeptide Reversed Phase $ 53 / 3 pk $ 39 / ea $ 4 / ea For use with silica columns for separation of proteins and peptides, such as Jupiter (); Vydac 8TP, 4TP (Alltech Associates, Inc.); SynChropak 3 C8, C4 (Eprogen, Inc.); Nucleosil 3 Å C8, C4 (Macherey-Nagel); Hypersil 3 Å (Thermo Hypersil-Keystone), and other widepore or 3 Å brands. Widepore C8 (DS, ctadecyl).5 - AJ-74 AJ-73 AJ-833 Widepore C5 (Pentyl).5 - AJ-737 Widepore C4 (Butyl).5 - AJ-75 AJ-73 AJ-834 Cartridges for Synthetic DNA / RNA Analysis $ 53 / 3 pk $ 39 / ea $ 4 / ea For use with columns like Clarity () ligo-rp (C8 TWIN Technology) - AJ-836 AJ-8 ligo-wax (WA, Weak Anion Exchanger).5 - AJ-835 AJ-8639 ligo-xt (DS, ctadecyl) - AJ-956 AJ-957 AJ-958 Cartridges for Silica GFC $ 39 / ea (Aqueous SEC) For use with silica GFC columns, such as Yarra and BioSep (); ZRBAX GF-Series (Agilent Technologies); Bio-Sil (Bio-Rad ). GFC AJ-8588 GFC AJ-8589 GFC AJ-859 Cartridges for Chiral $ 53 / 3 pk $ 39 / ea $ 4 / ea For use with chiral columns, such as Lux Cellulose-, -, -3, -4, i-cellulose-5, and Amylose-,- (); CHIRALCEL and CHIRALPAK (DAICEL Corporation) as noted on page 6. Lux i-cellulose-5 Cellulose tris(3,5-dichlorophenylcarbamate) - 9 AJ-8634 AJ-8635 Lux Cellulose- Cellulose tris(3,5-dimethylphenylcarbamate) - 9 AJ-844 AJ-845 AJ-846 Lux Cellulose- Cellulose tris(3-chloro-4-methylphenylcarbamate) - 9 AJ-8399 AJ-84 AJ-84 Lux Cellulose-3 Cellulose tris(4-methylbenzoate) - 9 AJ-863 AJ-864 AJ-865 Lux Cellulose-4 Cellulose tris(4-chloro-3-methylphenylcarbamate) - 9 AJ-868 AJ-869 AJ-863 Lux Amylose- Amylose tris(3,5-dimethylphenylcarbamate) - 9 AJ-9344 AJ-9338 AJ-9339 Lux Amylose- Amylose tris(5-chloro--methylphenylcarbamate) - 9 AJ-847 AJ-8473 AJ-8474 HPLC Guard Cartridge Holders* $ 7 / holder $ 365 /kit $ 45 /kit Reuseable Holder AJ-98 AJ-83 AJ-877 SFC Guard Cartridge Holders* $ 7 / holder $ 48 /kit $ 5 /kit Reuseable Holder AJ-98 AJ-867 AJ-868 * Includes column coupler PREP Preparative LC Column Protection If SecurityGuard PREP cartridge protection system does not perform as well or better than your current guard cartridge system of similar phase and dimensions, return the product with the comparative data within 45 days for a FULL REFUND. SecurityGuard PREP USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 33
34 rdering Information Achiral Phases Aeris Phase Length ID Part No. Price 5 µm PEPTIDE XB- 5. F-463-P-AX $,999 C8 5. G-463-P-AX 3,56 Kinetex Phase Length ID Part No. Price 5 µm XB-C8 5. B-465-P-AX $, B-465-U-AX 3,355. D-465-P-AX,589 3 D-465-U-AX Inquire 5. F-465-P-AX, F-465-U-AX Inquire 5. G-465-P-AX 3, G-465-U-AX Inquire EV C8 5. B-4633-P-AX, B-4633-U-AX 3,65. D-4633-P-AX,85 3 D-4633-U-AX Inquire 5. F-4633-P-AX 3,5 5 3 F-4633-U-AX Inquire 5. G-4633-P-AX 3, G-4633-U-AX Inquire Biphenyl. D-467-P-AX,589 5 D-467-V-AX Inquire 5. F-467-P-AX, F-467-U-AX Inquire 5. G-467-P-AX 3,59 HILIC. D-466-P-AX, F-466-P-AX, G-466-P-AX 3,59 C8 5. B-46-P-AX, B-46-U-AX 3,355. D-46-P-AX,589 3 D-46-U-AX Inquire 5. F-46-P-AX, F-46-U-AX Inquire 5. G-46-P-AX 3, G-46-U-AX Inquire C8 5. B-468-P-AX, B-468-U-AX 3,355. D-468-P-AX,589 3 D-468-U-AX Inquire 5. F-468-P-AX, F-468-U-AX Inquire 5. G-468-P-AX 3, G-468-U-AX Inquire Phenyl-Hexyl 5. B-463-P-AX, B-463-U-AX 3,355. D-463-P-AX,589 3 D-463-U-AX Inquire 5. F-463-P-AX, F-463-U-AX Inquire 5. G-463-P-AX 3, G-463-U-AX Inquire F5 5 3 B-474-U-AX 3,355 3 D-474-U-AX 4,749 NEW 5. F-474-P-AX, F-474-U-AX Inquire 5. G-474-P-AX 3, F-463-U-AX Inquire Gemini Phase Length ID Part No. Price 5 µm NX-C8 5. B-4454-P-AX $, B-4454-U-AX 3, C-4454-U-AX Inquire. D-4454-P-AX,85 3 D-4454-U-AX Inquire 5. F-4454-P-AX 3,5 5 3 F-4454-U-AX Inquire 5. G-4454-P-AX 3, G-4454-U-AX Inquire C8 5. B-4435-P-AX, B-4435-U-AX 3,375. D-4435-P-AX,69 3 D-4435-U-AX Inquire 5. F-4435-P-AX, F-4435-U-AX Inquire 5. G-4435-P-AX 3, G-4435-U-AX Inquire C6-Phenyl. D-4444-P-AX,69 5. F-4444-P-AX, G-4444-P-AX 3,549 µm NX-C8 5. B-4455-P-AX $,89. D-4455-P-AX,79 3 D-4455-U-AX 4,859 5 D-4455-V-AX Inquire 5. F-4455-P-AX 3, F-4455-U-AX Inquire 5 5 F-4455-V-AX Inquire 5. G-4455-P-AX 3, G-4455-U-AX Inquire 5 5 G-4455-V-AX Inquire C8. D-4436-P-AX,489 3 D-4436-U-AX Inquire 5. F-4436-P-AX, F-4436-U-AX Inquire 5 5 F-4436-V-AX Inquire 5. G-4436-P-AX 3, G-4436-U-AX Inquire 5 5 G-4436-V-AX,85 Jupiter Phase Length ID Part No. Price 4 µm Proteo 5 3 G-4396-U-AX Inquire µm Proteo. D-4397-P-AX $,49 5. G-4397-P-AX 3,7 5 3 G-4397-U-AX Inquire C8 3 Å 5 3 G-455-U-AX Inquire C4 3 Å 5. G-468-P-AX 3,7 For additional sizes not displayed, please contact your technical consultant or local distributor. If Axia packed columns do not provide at least an equivalent separation as compared to a competing preparative column of the same particle size, same phase, and dimensions, return the product with comparative data within 45 days for a FULL REFUND. nly applies to. mm ID columns. 34 USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web:
35 Tip: Luna Phase Length ID Part No. Price 5 µm C8() 5. B-45-P-AX $, B-45-U-AX 3, C-45-P-AX, C-45-U-AX Inquire. D-45-P-AX,69 3 D-45-U-AX Inquire 5. F-45-P-AX, F-45-U-AX Inquire 5. G-45-P-AX 3, G-45-U-AX Inquire C8() 75 3 C-449-U-AX Inquire 3 D-449-U-AX Inquire 5. F-449-P-AX, G-449-P-AX 3,59 CN 5. G-455-P-AX 3,59 Phenyl-Hexyl 5. F-457-P-AX,979 NH 5. F-4378-P-AX, G-4378-P-AX 3,59 HILIC. D-445-P-AX, F-445-P-AX, G-445-P-AX 3, G-445-U-AX Inquire PFP(). D-4448-P-AX,589 3 D-4448-U-AX Inquire 5. F-4448-P-AX, G-4448-P-AX 3, G-4448-U-AX Inquire Silica (). D-474-P-AX, F-474-P-AX, G-474-P-AX 3, G-474-U-AX Inquire µm C8() 5. B-453-P-AX $,75. D-453-P-AX, F-453-P-AX, F-453-U-AX Inquire 5. G-453-P-AX 3, G-453-U-AX Inquire 5 5 G-453-V-AX Inquire C8() 5. G-45-P-AX 3, G-45-V-AX Inquire Silica () 5. G-49-P-AX 3,49 5 µm C8() 5 5 G-473-V-AX Inquire C8() 5 5 G-47-V-AX Inquire Luna mega 5 µm Polar C8. D-4754-P-AX $,69 3 D-4754-U-AX 4,769 NEW 5. F-4754-P-AX, F-4754-U-AX Inquire 5. G-4754-P-AX Inquire 5 3 G-4754-U-AX Inquire 5 5 G-4754-V-AX Inquire PS C8 5. B-4753-P-AX, B-4753-U-AX 3,375. D-4753-P-AX,69 NEW 3 D-4753-U-AX Inquire 5. F-4753-P-AX Inquire 5 3 F-4753-U-AX Inquire 5. G-4753-P-AX Inquire 5 3 G-4753-U-AX Inquire 5 5 G-4753-V-AX Inquire Protect your Axia Prep Columns with SecurityGuard. See page 33 for ordering information Synergi Phase Length ID Part No. Price 4 µm Fusion-RP. D-444-P-AX $, F-444-P-AX, G-444-P-AX 3,469 Hydro-RP 5. B-4375-P-AX, F-4375-P-AX, G-4375-P-AX 3,469 Max-RP 5. F-4337-P-AX, G-4337-P-AX 3,469 Polar-RP 5. B-4336-P-AX,769. D-4336-P-AX,569 3 D-4336-U-AX Inquire 5. F-4336-P-AX, F-4336-U-AX Inquire 5. G-4336-P-AX 3,469 µm Fusion-RP 5. F-445-P-AX $,95 5. G-445-P-AX 3,65 Hydro-RP 5. F-4376-P-AX,95 5. G-4376-P-AX 3,65 Polar-RP 5. G-435-P-AX 3,65 Clarity Phase Length ID Part No. Price 5 µm ligo-rp. D-444-P-AX $,635 3 D-444-U-AX 4, G-444-P-AX 3,65 ligo-xt. D-4745-P-AX $,775 NEW 5. F-4745-P-AX 3, F-4745-U-AX 7, G-4745-P-AX Inquire µm ligo-rp 5. F-4445-P-AX $, F-4445-U-AX Inquire 5. G-4445-P-AX 3,79 ligo-wax 5. G-445-P-AX 3,65 Chiral Phases Lux Phase Length ID Part No. Price 5 µm Amylose- 5. F-473-P-AX Inquire 5. G-473-P-AX Inquire 5 3 G-473-U-AX Inquire 5 5 G-473-V-AX Inquire Amylose- 5. F-447-P-AX Inquire 5. G-447-P-AX Inquire 5 3 G-447-U-AX Inquire Cellulose- 5. F-4459-P-AX Inquire 5. G-4459-P-AX Inquire 5 3 G-4459-U-AX Inquire 5 5 G-4459-V-AX Inquire Cellulose- 5. F-4457-P-AX Inquire 5. G-4457-P-AX Inquire 5 3 G-4457-U-AX Inquire 5 5 G-4457-V-AX Inquire Cellulose-3 5. F-4493-P-AX Inquire 5. G-4493-P-AX Inquire 5 3 G-4493-U-AX Inquire 5 5 G-4493-V-AX Inquire Cellulose-4 5. F-449-P-AX Inquire 5. G-449-P-AX Inquire 5 3 G-449-U-AX Inquire 5 5 G-449-V-AX Inquire i-cellulose-5 5. F-4756-P-AX Inquire NEW 5. G-4756-P-AX Inquire 5 3 G-4756-U-AX Inquire 5 5 G-4756-V-AX Inquire USA Tel: Canada Tel: Puerto Rico Tel: Fax: info@phenomenex.com Web: 35
36 The Ultimate Pre-Packed Preparative HPLC/SFC Column PRSRT STD U.S. PSTAGE PAID TRRANCE, CA PERMIT N. 85 Australia t: +6 () f: +6 () Austria t: +43 ()-39-3 f: +43 ()-39-3 Belgium t: +3 () (French) t: +3 () (Dutch) f: +3 () beinfo@phenomenex.com Canada t: + (8) f: + (3) info@phenomenex.com China t: f: +86 () phen@agela.com Denmark t: f: nordicinfo@phenomenex.com Finland t: +358 () f: nordicinfo@phenomenex.com France t: +33 () 3 9 f: +33 () 3 9 franceinfo@phenomenex.com Germany t: +49 () f: +49 () anfrage@phenomenex.com India t: +9 ()4-3 4 f: +9 ()4-3 4 indiainfo@phenomenex.com Ireland t: +353 () f: eireinfo@phenomenex.com Luxembourg t: +3 ()3-487 f: +3 () nlinfo@phenomenex.com Mexico t: f: tecnicomx@phenomenex.com The Netherlands t: +3 ()3-487 f: +3 () nlinfo@phenomenex.com New Zealand t: +64 () f: +64 () nzinfo@phenomenex.com Norway t: f: nordicinfo@phenomenex.com Puerto Rico t: + (8) 54-HPLC f: + (3) info@phenomenex.com Spain t: f: espinfo@phenomenex.com Sweden t: +46 () f: nordicinfo@phenomenex.com United Kingdom t: +44 () f: +44 () ukinfo@phenomenex.com USA t: + (3) -555 f: + (3) info@phenomenex.com All other countries Corporate ffice USA t: + (3) -555 f: + (3) info@phenomenex.com Italy t: f: italiainfo@phenomenex.com products are available worldwide. For the distributor in your country, contact USA, International Department at international@phenomenex.com BR3997_us Terms and Conditions Subject to Standard Terms and Conditions which may be viewed at Trademarks Aeris, Clarity, Gemini, Kinetex, Luna, and Lux are registered trademarks, and BioSep, ligo-rp, ligo-wax, SecurityGuard, Synergi, TWIN-NX, TWIN Technology, Two-In-ne Technology, and Yarra are trademarks of. Angiomax is a registered trademark of The Medicines Company. Bio-Sil and Bio-RAd are registered trademarks of Bio-Rad Laboratories. NUCLESIL is a registered trademark of Macherey-Nagel. Hypersil is a registered trademark of Thermo Hypersil-Keystone LLC. SynChropak is a registered trademark of Eichrom Technologies, Inc. UPLC, XBridge, Symmetry, XTerra, and Waters are registered trademarks and BD and SymmetryShield are trademarks of Waters Corporation. ZRBAX is a registered trademark of Agilent Technologies, Inc. Vydac is a registered trademark of Alltech Associates, Inc. CHIRALPAK, CHIRALCEL, AD, AD-H, AD-RH, AY, AY-H, D, D-H, D-RH, J, J-H, J-RH, and Z-H are registered trademarks of DAICEL Corporation. Disclaimer Comparative separations may not be representative of all applications. is not affiliated with Waters Corporation, Agilent Technologies, Inc., Alltech Associates, Inc. or DAICEL Corporation. Columns used for comparison studies were manufactured by and purchased from Waters Corp., Agilent Technologies, Inc., Alltech Associates, Inc. and DAICEL Corporation. The opinions stated herein are solely those of the speaker and not necessarily those of any company or organization. Axia column and packing technology is patented by. U.S. Patent No. 7, 674, 383 Gemini and Kinetex EV are patented by. U.S. Patent Nos. 7,563,367 and 8,658,38 and foreign counterparts. 7, Inc. All rights reserved. 4 Madrid Avenue Torrance, CA USA
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