B6 Albino Mouse Models
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1 B6 Albino Mouse Models INTRODUCING TWO NEW B6 ALBINO MODELS ON THE C57BL/6NTac BACKGROUND FOR USE AS BLASTOCYST DONORS FOR B6 ES CELLS AND AS AN IMPROVED BACKGROUND FOR IMAGING OF B6 STRAINS: B6 ALBINO A ++ AND B6 ALBINO
2 B6 Albino A ++ C57BL/6NTac-A tm1.1arte Tyr tm1arte TACONIC MODEL NUMBER The B6 Albino A ++ mouse can save you valuable time and resources when generating your next mouse model. Since the B6 Albino A ++ mouse is an ideal blastocyst host for B6-derived ES cells, you can reduce the amount of time, labor, money, and animals used to generate your next mouse model. Chimeras made using the B6 Albino A ++ and B6 ES cells mated to B6 mice yield germline transmission pups you can identify by coat color alone. And G1 pups are identical to the B6 genome at all coat color loci, with no need for backcrossing. Use with ES cells derived from the C57BL/6NTac strain such as JM8 to produce coat color chimeras. The B6 Albino A ++ mouse can also be used with agouti B6 ES cells such as JM8A3. The B6 Albino A ++ mouse was developed by Taconic Biosciences and is available exclusively from Taconic. B6 B6 Albino A ++ contains two mutations, a reversion of the non agouti locus (a) to Agouti (A), plus a point mutation in the tryosinase locus. Inquire about available options such as superovulated females. To learn more, visit taconic.com/11227 Chimeras generated using B6-derived ES cells and blastocysts from the B6 Albino A ++ will be tri-colored. The agouti signaling peptide (produced in blastocyst-derived cells, which appear albino in the chimera) can produce a paracrine signaling effect in some cells derived from the ES cells. ES cell-derived hair patches can thus be either black or agouti. Both black and agouti patches should be counted for determination of chimerism percentages. TO ORDER
3 USE B6 ALBINO A ++ AS BLASTOCYST DONOR FOR BLACK JM8 ES CELLS This permits detection of germline transmission by coat color alone in 100% of pups. In addition G1 germline progeny are identical to C57BL/6NTac at all coat color loci. The B6 Albino A ++ mouse provides superior embryo yields compared to the BALB/c strain as well as more equal embryonic development. Overall, the combined advantages of using the B6 Albino A ++ mouse mean you can use fewer animals, while saving time, labor, and money. You can potentially skip a generation of breeding when mating B6 Albino A ++ chimeras to deleter strains on a B6 background. ES: C57BL/6NTac Coat color genetics: aabbcc HOST: C57BL/6NTac-A tm1.1arte Tyr tm1arte Coat color genetics: AABBcc C57BL/6NTac Coat color genetics: aabbcc NON-GERMLINE Coat color genetics: AaBBCc GERMLINE Coat color genetics: aabbcc 25 All Females Plug Positive Females BALB/cJBomTac B6 Albino A # Embryos / females B6 Albino A ++ provides superior embryo yields compared to the BALB/c strain. B6 Albino A ++ also shows more equal embryonic development, 0 morulae+blasts blasts morulae+blasts blasts with 70% harvested blastocysts at dpc 3.5, compared to 53% in BALB/cJBomTac. DISCUSS YOUR NEEDS
4 USE B6 ALBINO A ++ AS BLASTOCYST DONOR FOR AGOUTI JM8A3 ES CELLS This permits detection of germline transmission by coat color in 50% of pups. Black G1 germline progeny are identical to C57BL/6NTac at all coat color loci. Agouti G1 germline progeny are heterozygous at one coat color locus. ES: C57BL/6NTac Coat color genetics: AaBBCC HOST: C57BL/6NTac-A tm1.1arte Tyr tm1arte Coat color genetics: AABBcc C57BL/6NTac Coat color genetics: aabbcc NON-GERMLINE Coat color genetics: AaBBCc GERMLINE Coat color genetics: aabbcc and AaBBCC TO ORDER
5 B6 Albino C57BL/6NTac-Tyr tm1arte TACONIC MODEL NUMBER The B6 Albino mouse can save you valuable time and resources when generating your next mouse model. Mating chimeras to the B6 Albino provides a cost-effective approach for detecting germline transmission by coat color alone, and for maintaining the C57BL/6NTac substrain background. Use with agouti ES cells derived from the C57BL/6NTac strain such as JM8A3 to produce coat color chimeras. The B6 Albino mouse was developed by Taconic Biosciences, and is available exclusively from Taconic. To learn more, visit taconic.com/11971 DISCUSS YOUR NEEDS
6 USE B6 ALBINO AS BLASTOCYST DONOR FOR AGOUTI JM8A3 ES CELLS This permits detection of germline transmission by coat color in 100% of pups. G1 germline progeny are heterozygous at one or two coat color loci. The B6 Albino mouse is an ideal host for maintaining substrain specificity of ES cells derived from the C57BL/6NTac substrain. It may be used as a blastocyst host for B6 ES cells with an agouti mutation. It is also suitable for use as a genetic background for models in which a B6 background is needed with an albino coat, for example in imaging applications. ES: C57BL/6NTac Coat color genetics: AaBBCC HOST: C57BL/6NTac-Tyr tm1arte Coat color genetics: aabbcc C57BL/6NTac-Tyr tm1arte Coat color genetics: aabbcc NON-GERMLINE Coat color genetics: aabbcc GERMLINE Coat color genetics: aabbcc and AaBBcC TO ORDER
7 POSSIBLE MATING SCHEMES FOR CHIMERAS DERIVED FROM B6 ALBINO A ++ OR B6 ALBINO BLASTOCYSTS WITH JM8 AND JM8A3 ES CELLS ES CELL BLASTOCYST DONOR CHIMERA (G0) MATING PARTNER OFFSPRING (G1) COMMENTS NON-GERMLINE GERMLINE JM8 (Black) C57BL/6NTac- A tm1.1arte Tyr tm1arte Black/Agouti/ Albino C57BL/6NTac Agouti Black No genotyping required.* Black offspring are identical to C57BL/6NTac genome at all coat color loci. JM8A3 (Agouti) C57BL/6NTac- Tyr tm1arte No genotyping required.* Black are het (Cc). Agoutis are het (Aa, Cc). Agouti/Albino C57BL/6NTac- Albino Black or Agouti Tyr tm1arte JM8A3 (Agouti) C57BL/6NTac- A tm1.1arte Tyr tm1arte Agouti/Albino C57BL/6NTac Agouti Black or Agouti No genotyping required for black pups.* Black offspring are identical to C57BL/6NTac genome at all coat color loci. Germline agoutis are het (Aa). JM8A3 (Agouti) C57BL/6NTac- A tm1.1arte Tyr tm1arte No genotyping required.* Agoutis are het (Cc) or double het (Aa, Cc). Agouti/Albino C57BL/6NTac- Albino Agouti A tm1.1arte Tyr tm1arte *No genotyping is required to identify germline pups derived from ES cells; these can be identified by coat color. However, when using ES cells which are heterozygous for a mutation, genotyping is still required to determine which germline pups carry the mutation. KEY: COAT COLOR Albino Black Black/Agouti/Albino Agouti/Albino Agouti RECOMMENDATIONS FOR BLACK JM8 ES CELLS: Use Taconic B6 Albino A ++ (C57BL/6NTac-A tm1.1arte Tyr tm1arte ) as blastocyst donor and mate chimeras to C57BL/6NTac. FOR AGOUTI JM8A3 ES CELLS: Use Taconic B6 Albino (C57BL/6NTac-Tyr tm1arte ) as the blastocyst donor and mate chimeras to Taconic B6 Albino (C57BL/6NTac-Tyr tm1arte ). Or use Taconic B6 Albino A ++ (C57BL/6NTac-A tm1.1arte Tyr tm1arte ) as blastocyst donor and mate chimeras to your choice of C57BL/6NTac or Taconic B6 Albino A ++ (C57BL/6NTac-A tm1.1arte Tyr tm1arte ). FURTHER READING: Zevnik B, Uyttersprot NC, Perez AV, Bothe GW, Kern H, Kauselmann G. (2014) C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. PLoS One. 9(3):e DISCUSS YOUR NEEDS
8 Take Your Research Further ADDITIONAL SERVICES GEMS DESIGN Taconic Biosciences GEMS Design empowers our clients to develop research models specifically suited to the unique needs of their discovery and development studies or therapeutic programs. Gene Inactivation Gene Mutation or Replacement CRISPR Gene Editing Transgene Expression mirna expression Cohort Production Packages HUMANIZATION Taconic offers both genetic and cell and tissue based humanization of mouse models. Humanized mouse models are increasingly being utilized for a variety of research applications. Genetically Humanized Mice Cell and Tissue Humanized Mice GEMS MANAGEMENT Taconic s fully integrated GEMS Management brings innovative models from design to study-ready cohorts with unprecedented speed and transparency. Embryology Rapid Colony Expansion Contract Breeding Surgical Services Tissue Collection Genotyping and Molecular Analysis Microbiome and Germ Free Research Models and Services CHOOSE TACONIC For more than 60 years, Taconic has anticipated the needs of the scientific community to deliver models and services that meet the diverse needs of biomedical and biopharmaceutical researchers. Today that forward thinking and commitment to working collaboratively has resulted in a client-centric environment infused with a knowledge bank that allows you to draw on informed insight about next generation GEMS and humanized mouse models, enabling fast and reliable outcomes in your research. YOUR COLLABORATIVE PARTNER As a full-service biosciences company, Taconic can help you acquire, test, develop, breed, cryopreserve, prepare, and distribute highly relevant research lines worldwide. Whether you require custom genetically engineered, humanized or research-ready models, Taconic s scientists will partner with you to rapidly and efficiently deliver the highest quality models. TALK TO A SCIENTIST Our scientific teams are happy to meet and talk with you about the most efficient way to achieve your study goals. Working in partnership with clients the world over, our scientific teams offer expert advice that can help you speed up your research and reduce your overall costs. TALK TO A REPRESENTATIVE For general information, you can talk to a member of our customer service team. Our customer service team is here to help you make the right decisions and get the models you need fast. Contact us at info@taconic.com VISIT TACONIC.COM For more information on the entire Taconic portfolio of products and services designed to help further your research, visit taconic.com Taconic Biosciences, Inc. All rights reserved. Contents of this publication may not be reproduced in any form without prior permission. US: EU: INFO@TACONIC.COM TACONIC.COM 0915
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