Production of Biofeeds, Biofuels and Biochemicals from CO 2 using Hydrogen Bacteria
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1 5.3 Microbial and Synthetic Approaches to CO 2 Utilization, 2017 BIO World Congress Production of Biofeeds, Biofuels and Biochemicals from CO 2 using Hydrogen Bacteria July 24, 2017 Dr. Hideaki Yukawa CEO, Utilization of Carbon Dioxide Institute Co., Ltd.
2 UCDI s Business Plan Convert CO 2 into high-value products Contribution to food security and global warming problems Copyright 2017 by UCDI All Rights reserved. 1
3 UCDI s Platform: Hydrogen Bacteria Hydrogenophilus thermolutelus Copyright 2017 by UCDI All Rights reserved. 2
4 What are Hydrogen Bacteria? Copyright 2017 by UCDI All Rights reserved. 3
5 NASA: Exploring the possibility of using Hydrogen Bacteria as a protein production for space flight Conclusion: 20L-culture could support one Astronaut Biotech. and Bioengi. vol-8,217(1966) Copyright 2017 by UCDI All Rights reserved. 4
6 History of UCDI s Hydrogen Bacteria Isolation from Nature 1970s: Dr. Kodama s team, Applied Microbiology Lab., University of Tokyo, had tried to isolate many kinds of hydrogen bacteria from nature. Scientific Advisor Dr. Toru Kodama They set up a landmark of hydrogen bacteria s feature for future industrialization. * Rapid growth in a complete inorganic medium Target of doubling time : less than 1 hour * Thermophilic hydrogen bacteria Target of optimum temperature: over 50 C Copyright 2017 by UCDI All Rights reserved. 5
7 History of UCDI s Hydrogen Bacteria Izu Peninsula Tokyo The targeted strain was isolated from a hot spring in Izu Peninsula Screening Result 1976: Dr. Kodama s team got big success The strain s feature Doubling time: 1hour Optimum temperature: 52 C Optimum ph: 7.0 Gram- negative Strictly aerobic Taxonomic position: Pseudomonas hydrogenothermophila Agric. Biol. Chem., 41,685 (1977) By the detailed taxonomic study: Hydrogenophilus thermolutelus Int. J. Syst Bacteriol.,49,783 (1999) Copyright 2017 by UCDI All Rights reserved. 6
![[archive] History of UCDI s Hydrogen Bacteria Japanese News Paper Asahi took up the research project](/docs-images/77/74536751/images/8-1.jpg "in both article and social cartoon(1978) Hydrogen Bacteria is rich nutrition Protein content: 80%")
8 [archive] History of UCDI s Hydrogen Bacteria Japanese News Paper Asahi took up the research project in both article and social cartoon(1978) Hydrogen Bacteria is rich nutrition Protein content: 80% Continuous production process is practicable Feed test using poultry was done successfully Social cartoon Dinner in future Hydrogen Bacteria on the dinner table Copyright 2017 by UCDI All Rights reserved. 7
9 World Population Forecast Now: More than 7 B people inhabit our planet Future: Over 9B or close to 10 B by the year 2050 This sharp increase in global population is the root cause of various issues facing our planet, including environmental pollution and food security. Source: Ministry of Agriculture, Forestry and Fisheries Copyright 2017 by UCDI All Rights reserved. 8
10 Biofeeds: Single Cell Protein High protein-score/ High cell density Hydrogenophilus thermolutelus UCDI strain Hydrogenophilus thermolutelus Before inoculation 10hrs incubation High growthrate: dubling time 1hour High Amino Value Non-GMO Natural protein source Copyright 2017 by UCDI All Rights reserved. 9
11 Biofeeds: Production Process Copyright 2017 by UCDI All Rights reserved. 10
12 Biofeeds: Aquaculture Fields Fishers and aquaculture: Important sources of food and nutrition World per capita fish supply 20kg in 2014 Thanks to Aquaculture Aquaculture provides half of all fish Source: FAO Fish State Copyright 2017 by UCDI All Rights reserved. 11
13 How to keep sustainable aquaculture? Fishmeal Production and Price Fish feed: the biggest cost in the production of aquaculture Fishmeal is main ingredient 50~70%W/W in fish feed Copyright 2017 by UCDI All Rights reserved. 12
14 UCDI s Biofeeds : Business Plan Plant engineering : UCDI collaborating with major engineering companies Market : aquaculture, chicken, pork Supply and Demand (Million ton) Growth rate Chicken % Pork % Source: Policy Research Institute, Ministry of Agriculture, Forestry and Fisheries (PRIMAFF) Copyright 2017 by UCDI All Rights reserved. 13
15 rdna Research Established main generic tools Genetic manipulation both in autotrophic and heterotrophic culture Gene transfer Plasmid vectors Introduction into chromosome by homologous recombination Transformation by electroporation Selection Positive selection by antibiotics resistance kanamycin, hygromycin, etc. Negative selection with sacb gene Reporter gene Beta-galactosidase from Thermus therm ophilus (e.g. for screening of promoters) Counter selection Copyright 2017 by UCDI All Rights reserved. 14
16 rdna Research Set up Industrial host cell for cell-factory Copyright 2017 by UCDI All Rights reserved. 15
17 Biocatalysts: genetically modified strains Business Plan Butanol : Material for Jet Fuel Amino Acids: Feed Additives (Trp, Met. etc.) Amino Acid Organic Acids/Aromatic compounds : Materials for Polymer We are looking for business partners!! Copyright 2017 by UCDI All Rights reserved. 16
18 About Us Established : August 12, 2015 Paid-in Capital : Head Office : Representative : US$1M Minato-ku, Tokyo Dr. Hideaki Yukawa, CEO Research Facility : Campus of University of Tokyo Mission : Contribution to Food Security and Global Warming Problems Copyright 2017 by UCDI All Rights reserved. 17
19 Head Office CEO Dr. Hideaki Yukawa Scientific Advisor Dr. Toru Kodama Special Advisor Dr. Kazuo Nagai Director Ms. Tomoko Yukawa Executive Officer Mr. Hiroshi Maeda Manager Ms. Setsuko Beppu Manager Ms. Sumie Honma Manager Ms. Hikari Miyazaki Manager Ms. Ryoko Senoo Copyright 2017 by UCDI All Rights reserved. 18
20 Research Team Alignment : Applied Microbiology Lab. University of Tokyo Professor, PhD Masaharu Ishii rdna works Fermentation works Senior Researcher Dr. Naoto Ohtani Researcher Dr. Shota Oku Researcher Dr. Kenro Shinagawa Copyright 2017 by UCDI All Rights reserved. 19
21 Thank you Please contact to Copyright 2017 by UCDI All Rights reserved. 20
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