EVALUATION OF THEPRECISIONID SYSTEM FOR TARGETED SEQUENCING OF DNA AND RNA MARKERS FOR HUMAN IDENTITY AND BODY FLUID IDENTIFICATION
|
|
|
- Mitchell Wright
- 6 years ago
- Views:
Transcription
1 EVALUATION OF THEPRECISIONID SYSTEM FOR TARGETED SEQUENCING OF DNA AND RNA MARKERS FOR HUMAN IDENTITY AND BODY FLUID IDENTIFICATION www. tourisminbarcelona.com Jack Ballantyne HIDS, Barcelona, Spain 2016
2 Next Generation Sequencing NGS is set to revolutionize forensic biology Enabling the recovery of more identifying and intelligence information From a wide variety of different single, mixed source and degraded samples It will be several years before NGS totally supplants current CE technology However, labs can TODAY begin to use NGS for certain cases Significant advancements in: Automation of library and template preparation and sequencing Forensic sequencing kits Software solutions for downstream genotyping analysis Ion Chef TM System and Ion S5 TM System DNA analysis for human identification RNA analysis for body fluid identification
3 Evaluation of the EA Precision ID GlobalFiler Mixture ID Panel, Ion S5 System and Ion Chef System
4 Evaluation of the EA Precision ID GlobalFiler Mixture ID Panel, Ion S5 System and Ion ChefSystem
5 Ion Chef System Automates library, template preparation, & chip loading! Library Preparation Template Preparation Running Complete Oh no!!
6 Which Chef would you prefer? Automated ~15 min or less prep time Fewer errors! Manual/Hands-on Hundreds of steps days to complete Error-prone?
7 Manual preparation Library Preparation Template Preparation OT2 templating (overnight) ES - enrichment Manually load chip (with foam?!)
8 Action 1 Action 2 Action 3 Add 8 samples (1ng/sample) to 96-well plate Add Precision ID Panel to Reagents Cartridge Snap 5 cartridge components onto Ion Chef deck Pipette samples* Pipette primers L i b r a r y * Assume use of multichannel pipette 96-well plate with IonCode Barcode Adapters Reagents Cartridge Reagents Cartridge 3 2 Solutions Cartridge Tips cartridge 1 96-well sample plate 5 Enrichment Cartridge ~7.5 hrs Action 1 Action 2 Action 3 Plan Run in Ion Torrent Broweser Add Libraries to Reagents Cartridge Snap cartridge components onto Ion Chef deck T e m p l a t e Pipette libraries Reagents Cartridge Reagents Cartridge Solutions Cartridge 3 2 Tips cartridge 1 Chips 96-well sample plate 5 Enrichment Cartridge Dip trays, centrifuge lids, swinging buckets ~13.5 hrs
9 While the Chef is working. With the Ion Chef System With the Manual Chef Hands-off time while the chef is running Work does not pile up Time for a break! Hands-on time in the lab Work piles up!!! No time for a break!
10 Ion S5 System Load-and-go reagents Straightforward user interface Less than 15 min of hands-on time
11 EARLY ACCESSPRECISIONID GLOBALFILER MIXTUREID PANEL IONCHEF SYSTEM IONS5 SYSTEM CONVERGE TM SOFTWARE
12 EA Precision ID GlobalFiler Mixture ID Panel Type of Target Number of Targets Autosomal STRs 29 Y-STRs 1 Autosomal SNPs 42 Y-SNPs 2 Indels Microhaplotypes 2 (Amel and Y indel) 36 clusters of 2 to 4 SNPs 113 targets in the human genome in a single reaction!! Early Access Ion Chef and Ion S5 reagents Ion 530 Chip Designed to work on challenging samples that perform poorly in CE systems
13 Testing & Evaluation Studies Study # Samples Library quant (pm) Chip loading # Reads Total Bases Concordance % 4.1 M 541 M Sensitivity (250pg, 125pg) Casework samples % 4.0M 520 M % 10.7M 1.22 G 8 in progress Mixture studies to be completed next: Male/female Male/female multiple contributor
14 Data exported directly into CONVERGE software
15 Concordance 8 single source samples 5 male, 3 female Compared to CE GlobalFiler results All GlobalFiler loci concordant with NGS panel!
16 Sensitivity Autosomal STRs Sample S2 Female S3 Male S4 Female S9 Male Input (pg) Total Reads Avg. Reads (Per Locus) , , , , , , , , STRs Min Reads (Locus) 893 D22S D22S D22S D22S D22S D22S D19S D22S1045 Max Reads (Locus) 6637 TH TH TH TPOX 7963 TPOX 9291 TPOX 5665 TH TH01 Total reads = main alleles only (doesn t include stutter)
17 16 Casework Samples Libraries combined to run 16 samples on one Ion 530 Chip 6 blood 5 saliva 2 semen 1 menstrual blood 2 vaginal 9 unique donors - some samples from the same donor Full STR profiles from 15/16 samples Spit on pavement partial profile; low level mixture (a lot of information still obtained!)
18 Blood Car Back Seat 1 week! Full profiles: STR, SNP, MH
19 Vaginal swab 37 o C 2 years! Full profiles: STR, SNP, MH
20 Chewing gum (~4-5 days old) Full profiles: STR, SNP, MH New STR New STR New STR New STR
21 Spit on pavement GlobalFiler results D3S1358 TPOX 16, 17 identified in both systems NGS additional 16 (second donor?) NGS 8 allele not detected with GlobalFiler result
22 Extra Discrimination Sequence Level Hetero-homozygous (as Rob calls it) Compound heterozygous (as the rest of the World calls it)
23 Extra Discrimination Sequence Level 19 stutter (from 20 allele) different sequence than main 19 allele
24 Additional Identity Information -SNPs
25 Additional information -MH MH low level 2 person mixture ( spit on pavement) Will be especially used in mixture studies to help in determining # of donors!
26 TARGETED RNA EXPRESSION FOR BODY FLUID IDENTIFICATION ION CHEF SYSTEM ION S5 SYSTEM Collaboration with University of Zurich
27
28 Body Fluid/Tissue Source ID Whole Transcriptomeversus Targeted RNA re-sequencing Digital gene expression (DGE)-counts how many copies of a transcript in a sample Facilitates quantitative approaches Can detect mixtures After cdna formation, same process as gdna Multiplex sequence analysis-multiple genes in multiple samples (and RNA + DNA co-analysis?) Associate a body fluid biomarker with a DNA profile (i.e. the donor)?
29 Methods Ion AmpliSeq TM Kit for Chef DL8 Kit SuperScript TM VILO TM 20ng total RNA Custom Ion AmpliSeq Primer Pool Ion 520/530 Kit-Chef
30 Run Data Ion Chef System-Ion S5 System
31 Specificity By PRM2 Only detected in semen samples Gene By Sample Blood Sample - Blood Specific Genes Strongly Expressed
32 Blood Semen Menstrual Saliva: ~64%, Vaginal: ~63-84%, Skin: ~65% genes removed in new primer pool to improve specificity
33 Autosomal NGS STR profiles concordant with CE methods easily obtained with few minutes hands-on effort using the automated Ion Chef System/Ion S5 System/Precision ID GlobalFiler Mixture ID Panel/Converge Software/1 ngdna from casework type samples apart from 29 astrs, the genotypes of 42 asnpsand 36 MH s also obtained RMP from the asnpsare typically Increased variation at STR loci detected and can be used Hetero-homozygotes Stutter peak distinguished from allele peak when indistinguishable by CE Modular RNA-based body Fluid ID system being developed to be compatible for the automated Ion Chef System and Ion S5 System shows early promise Next step: MIXTURES here we come!
34 Acknowledgements Dr. Erin Hanson (Co-Chef) (who hates having her picture taken) The amazing Thermo Fisher Scientific team! Sheri, Rob, Joe, Matt, Shelly Sharada, Jie, Ravi, Narsi & the SW team! Nicolette, Tanya Body fluid Cordula Haas, Sabrina Ingold (University of Zurich) Funding: NIJ, EUROFORGEN
35 Thank you for your attention!
36 The Precision ID GlobalFiler Mixture ID Panel is in early access development and not available for purchase. The content provided herein may relate to products that have not been officially released and is subject to change without notice. For Forensic or Paternity Use Only. When used for purposes other than Human Identification the instruments and software modules cited are for Research Use Only. Not for use in diagnostic procedures. Speaker was provided travel and hotel support by Thermo Fisher Scientific for this presentation, but no remuneration
Forensic Applications of CE and MPS
Forensic Biology Polymorphic Enzyme Single Locus PCR RFLP Massively Parallel Sequencing (MPS) Ion Chef System Ion S5 System Ion Chef System Prepare barcoded DNA libraries with Applied Biosystems Precision
Introducing the Applied Biosystems TM Precision ID NGS System for Human Identification
Introducing the Applied Biosystems TM Precision ID NGS System for Human Identification Sheri J. Olson Director, Product Management Casework Human Identification Current challenges for forensic scientists
Expand your forensics workflow with the Precision ID NGS System for human identification
Expand your forensics workflow with the Precision ID NGS System for human identification Integrate NGS into your forensic DNA lab workflow The Applied Biosystems Precision ID NGS System for human identification
Expand your forensics workflow with the Precision ID NGS System for human identification with mtdna, SNP, and STR analysis
Expand your forensics workflow with the Precision ID NGS System for human identification with mtdna, SNP, and STR analysis Integrate NGS into your forensic DNA lab workflow The Applied Biosystems Precision
Exploring of microrna markers for body fluid identification using NGS
Exploring of microrna markers for body fluid identification using NGS Zheng Wang, Yiping Hou Institute of Forensic Medicine Sichuan University, China Barcelona May, 11, 2016 Outline Introduction of Institute
Evaluation of NGS technology for identification, ancestry prediction and phenotypic characters prediction with the Ion PGM System
Evaluation of NGS technology for identification, ancestry prediction and phenotypic characters prediction with the Ion PGM System Who we are? IGNA (Nantes Atlantic Genetic Institute) was created in 2003
Get more information from challenging samples with next-generation sequencing of short tandem repeats
brand bar placeholder APPLICATION NOTE Precision ID NGS System Get more information from challenging samples with next-generation sequencing of short tandem repeats In this application note, we demonstrate:
From Genetics to Genomics European Perspectives for an Integrated Approach to the Use of Genetic Evidence in Criminal Investigations
From Genetics to Genomics European Perspectives for an Integrated Approach to the Use of Genetic Evidence in Criminal Investigations Peter M. Schneider Institute of Legal Medicine University of Cologne
The injection of the 28 and 29 PCR cycle data was performed at 3kV for 10 and 5 seconds.
TECHNICAL NOTE Evaluation of a decreased cycle number (28 cycles) and decreased injection time (5 seconds) when the GlobalFiler PCR Amplification Kit is run on a 3130xl Genetic Analyzer The performance
DNA 101. DNA 101:Kate Philpott and Jennifer Friedman
DNA 101 DNA 101:Kate Philpott and Jennifer Friedman DNA found in cells of your body cell nucleus DNA Found in cells once they leave your body too CONSIDER THE SOURCE Blood* Semen* Hair Bone Saliva* Urine*
Ion S5 and Ion S5 XL Systems
Ion S5 and Ion S5 XL Systems Targeted sequencing has never been simpler Explore the Ion S5 and Ion S5 XL Systems Adopting next-generation sequencing (NGS) in your lab is now simpler than ever The Ion S5
From Forensic Genetics to Genomics Perspectives for an Integrated Approach to the Use of Genetic Evidence in Criminal Investigations
From Forensic Genetics to Genomics Perspectives for an Integrated Approach to the Use of Genetic Evidence in Criminal Investigations Peter M. Schneider Institute of Legal Medicine University of Cologne
Ion S5 and Ion S5 XL Systems
Ion S5 and Ion S5 XL Systems Targeted sequencing has never been simpler Explore the Ion S5 and Ion S5 XL Systems Adopting next-generation sequencing (NGS) in your lab is now simpler than ever The Ion S5
Upgrading the Workflow: Enhancing Information Recovery from Forensic Evidence. The world leader in serving science
Upgrading the Workflow: Enhancing Information Recovery from Forensic Evidence The world leader in serving science The GlobalFiler Kit: Going Further for Casework Powered by 6-Dye Chemistry 2 What is the
Validation of Identity and Ancestry SNP Panels for the Ion PGM
Validation of Identity and Ancestry SNP Panels for the Ion PGM Christopher Phillips, Carla Santos, Maria de la Puente, Manuel Fondevila, Ángel Carracedo, Maviky Lareu Forensic Genetics Unit, University
Investigator Quantiplex HYres Kit
These specifications are for the procurement of a validation study at GBI Division of Forensic Sciences facilities of two commercially available kits used in forensic DNA analysis. The two kits to be validated
PowerSeq 46GY System Eases the transition to MPS for forensic laboratories
PowerSeq 46GY Eases the transition to MPS for forensic laboratories The Power to Solve...from Sample to Analysis Coming Soon! The Power to Solve... from Sample to Analysis The Promega PowerSeq s enable
Genetic Identity. Steve Harris SPASH - Biotechnology
Genetic Identity Steve Harris SPASH - Biotechnology Comparison of Organisms ORGANISM GENES BASE PAIRS Lambda Phage 40 50,000 E.coli 400 5,000,000 Yeast 13,000 15,000,000 Human 20,000 3,000,000,000 (3 billion)
AmpF STR NGM PCR Amplification Kit - Overview
AmpF STR NGM PCR Amplification Kit - Overview The most advanced STR kits optimized for analysis of forensic casework and database samples in Europe Data Quality Worth Sharing! 9/7/2011 Life Technologies
Centre of Forensic Sciences
Centre of Forensic Sciences Technical Information Sheets DNA Information Introduction DNA is the genetic blueprint of life and is packaged into paired structures known as chromosomes. One from each pair
Ion S5 and Ion S5 XL Systems
Ion S5 and Ion S5 XL Systems Targeted sequencing has never been simpler Introducing the Ion S5 and Ion S5 XL systems Now, adopting next-generation sequencing in your lab is simpler than ever. The Ion S5
What is DNA? Deoxyribonucleic Acid The inherited genetic material that makes us what we are
DNA Basic Genetics What is DNA? DNA is Deoxyribonucleic Acid The inherited genetic material that makes us what we are DNA in the Cell Human Genome ~3 billion base pairs of DNA 30,000-35,000 genes Population-each
First Look: Applied Biosystems NGM Detect PCR Amplification Kit
First Look: Applied Biosystems NGM Detect PCR Amplification Kit Lisa Calandro, HID Professional Services Leader Human Identification Business The world leader in serving science Here s what we ve heard
Human Identification Solutions Conference, Mar , Madrid Quantifiler Trio kit and forensic samples management: a matter of degradation
Human Identification Solutions Conference, Mar 4 2015, Madrid Quantifiler Trio kit and forensic samples management: a matter of degradation Stefano Vernarecci Forensic Genetics Laboratory Scientific Police
Procedure for Casework DNA Interpretation
Procedure for Casework DNA Interpretation 1.0 Purpose The purpose of this document is to provide guidelines for the interpretation of autosomal DNA results when amplified with Identifiler Plus. 2.0 Scope
GenPlex HID Training Class I
Rixun Fang GenPlex HID Training Class I Outline of Presentation Introduction GenPlex HID kit Experimental plan Class schedule Forensic SNP Analysis GenPlex HID Training Class I 2 Potential Forensic Applications
GlobalFiler Kit Casework Test Site Results and Other Development Updates
GlobalFiler Kit Casework Test Site Results and Other Development Updates April Orbison Sr. Field Applications Specialist Human Identification 1 Agenda GlobalFiler Kit Update: Recent Casework Test Site
High Cross-Platform Genotyping Concordance of Axiom High-Density Microarrays and Eureka Low-Density Targeted NGS Assays
High Cross-Platform Genotyping Concordance of Axiom High-Density Microarrays and Eureka Low-Density Targeted NGS Assays Ali Pirani and Mohini A Patil ISAG July 2017 The world leader in serving science
DNA Methylation Profiling Using SNaPshot Multiplex
DNA Methylation Profiling Using SNaPshot Multiplex This picture is the property of the Genetic Science Learning Center at the University of Utah How to set up DNA methylation profiling in your lab Introducing
Development and characterization of a high throughput targeted genotypingby-sequencing solution for agricultural genetic applications
Development and characterization of a high throughput targeted genotypingby-sequencing solution for agricultural genetic applications Michelle Swimley 1, Angela Burrell 1, Prasad Siddavatam 1, Chris Willis
Product Catalog 2012 AmplGen Ltd.
Professional solutions for Genetic Human Identification and Molecular Diagnostics Product Catalog 2012 AmplGen Ltd. Riga, Lativa 2 Catalog AmplGen 2012, ver. #02 Dear colleagues, AmplGen is new European
PowerPlex Fusion 6C System
PowerPlex Fusion 6C System Marty Ensenberger, Ph.D. October 8 th 2014 Disclaimer PowerPlex Fusion 6C is still in development. It is possible that small changes (to protocol, etc.) occur between now and
Routine Use of ForenSeq Solution on Casework Samples: Feedback One Year After Lab Implementation
Routine Use of ForenSeq Solution on Casework Samples: Feedback One Year After ab Implementation ille DEII 2018 - Barcelona Paris yon François-Xavier AURENT, Ph.D Head of R&D in Forensic Genetics Institut
Sequencing and PCR. Training: Ion S5 and S5 XL Systems workflow training
Training: Ion S5 and S5 XL Systems workflow training This interactive course focuses on the Ion S5 and Ion S5 XL Systems operation and the Ion AmpliSeq workflow on the Ion Chef System for sequencing. The
GlobalFiler TM Extra Cycle Evaluation Report
GlobalFiler TM Extra Cycle Evaluation Report Table of Content Chapter 1 Summary 1. Introduction... 2 2. Systems, Kits and Conditions... 3 3. Studies... 4 Chapter 2 Minimum Threshold and Background Study
Development of a Global STR Multiplex for Human Identification Analysis. Julio Mulero, Ph. D. R&D Group Human Identification, Life Technologies
Development of a Global STR Multiplex for Human Identification Analysis Julio Mulero, Ph. D. R&D Group Human Identification, Life Technologies Agenda Why a global multiplex? Multiplex Configuration Strategy
Converge Software. A comprehensive forensic analysis platform that integrates answers for you
PRODUCT BULLETIN Human identification Converge Software A comprehensive forensic analysis platform that integrates answers for you Key features and benefits Analyze next-generation sequencing data from
Artifacts Identified Post-Developmental Validation: AmpFLSTR Identifiler Plus PCR Amplification Kit
March 29, 2018 TECHNICAL NOTE Artifacts Identified Post-Developmental Validation: AmpFLSTR Identifiler Plus PCR Amplification Kit The purpose of this document is to assist with data interpretation by providing
NEW TECHNOLOGIES - AN INTRODUCTION
NEW TECHNOLOGIES - AN INTRODUCTION Niels Morling, MD DMSc Professor of Forensic Genetics Chairman & Director of the Department of Forensic Medicine Faculty of Health and Medical Sciences University of
Virginia and the New SWGDAM Guidelines
DNA Mixture Interpretation Workshop Brad Jenkins March 16, 2011 Virginia and the New SWGDAM Guidelines Biology Program Manager, Virginia Department of Forensic Science NIJ Disclaimer This project was supported
Exploring MPS for RNA profiling. Titia Sijen
Exploring MPS for RNA profiling Titia Sijen t.sijen@nfi.minvenj.nl When cells have a specialized function, there must be special molecules: Messenger RNA Micro RNA Epigenetic Proteome biochemical Microbial
Development of a Global STR Multiplex for Human Identification Analysis
Development of a Global STR Multiplex for Human Identification Analysis ISHI 2012 Dennis Wang Sr. Staff Scientist Human Identification Group 1 Agenda Why a Global Multiplex? Multiplex Configuration Strategy
Development of a Global STR Multiplex for Human Identification Analysis
Development of a Global STR Multiplex for Human Identification Analysis Jonathan Tabak Life Technologies 1 Agenda Introduction Multiplex Configuration Strategy Application-Specific Development and Testing
Innovations in quantitative and qualitative forensic assessment of human DNA Streamlining STR & Y STR profiling workflow
Innovations in quantitative and qualitative forensic assessment of human DNA Streamlining STR & Y STR profiling workflow Esperanza Gonzalez, Ana Carrillo, Mª Carmen Periñan, Esther Navajo, and Antonio
Molecular tools for forensic
Molecular tools for forensic body fluid identification Hwan Young Lee, Ph.D. Department of Forensic Medicine, Yonsei University College of Medicine, Seoul, Korea Presentation overview Introduction: Body
GENOTYPING-BY-SEQUENCING USING CUSTOM ION AMPLISEQ TECHNOLOGY AS A TOOL FOR GENOMIC SELECTION IN ATLANTIC SALMON
GENOTYPING-BY-SEQUENCING USING CUSTOM ION AMPLISEQ TECHNOLOGY AS A TOOL FOR GENOMIC SELECTION IN ATLANTIC SALMON Matthew Baranski, Casey Jowdy, Hooman Moghadam, Ashie Norris, Håvard Bakke, Anna Sonesson,
Guidelines and Considerations for Evaluating or Validating Extraction Chemistry
Guidelines and Considerations for Evaluating or Validating Extraction Chemistry Introduction In order to evaluate a DNA extraction method effectively, it is imperative to consider all aspects of chemistry
Validation Studies on DNA Mixtures Using the PowerPlex Fusion 6C System
Promega Webinars eries: Genetic Identity INAEX: Improving NA ata Exchange Validation tudies on NA Mixtures Using the PowerPlex Fusion 6C ystem INTCF-Madrid ERVICIO E BIOLOGIA upported by a grant from the
Surely Better Target Enrichment from Sample to Sequencer
sureselect TARGET ENRICHMENT solutions Surely Better Target Enrichment from Sample to Sequencer Agilent s market leading SureSelect platform provides a complete portfolio of catalog to custom products,
STR Interpretation Guidelines
Introduction: STR Interpretation Guidelines The interpretation of results in casework is necessarily a matter of professional judgment and expertise. Not every situation can or should be covered by a pre-set
King s Research Portal
King s Research Portal DOI: 10.1016/j.fsigen.2015.04.007 Document Version Peer reviewed version Link to publication record in King's Research Portal Citation for published version (APA): Eduardoff, M.,
Development of New Technologies for DNA Analysis of Challenging Forensic Samples
Development of New Technologies for DNA Analysis of Challenging Forensic Samples Sudhir K. Sinha, Ph.D InnoGenomics Technologies, LLC 1441 Canal Street, Suite 307 New Orleans, LA 70112 Commercial interest
QIAcube Pure Efficiency
Matt Kramer QIAcube Pure Efficiency QIAcube Overview What is it all about? Key features and capabilities Application data & summary 2 QIAcube : What Is It All About? 3 How Everything Began The Evolution
Development of an RNA-based screening assay for forensic stain identification
Development of an RNA-based screening assay for forensic stain identification 1 Trisha L. Noreault-Conti, Ph.D. and 1 Eric Buel, Ph.D. 1 Vermont Forensic Laboratory, Department of Public Safety, 103 South
Exploring the Capabilities of Mixture Interpretation Using True Allele Software
24th Congress of the International Society for Forensic Genetics Vienna, Austria O-42 Exploring the Capabilities of Mixture Interpretation Using True Allele Software Michael D. Coble and John M. Butler
Analytical verification methods for the Oncomine Lung cfdna Assay using the Ion S5 XL System
WHITE PAPER Oncomine Lung cfdna Assay and Ion S5 XL System Analytical verification methods for the Oncomine Lung cfdna Assay using the Ion S5 XL System Key highlights Investigate tumor heterogeneity and
Implementation of MPS into a Specialist Casework Laboratory. David Ballard
Implementation of MPS into a Specialist Casework Laboratory David Ballard DNA Evidence to Investigative Insights Berlin, 16 Outline ForenSeq STR Validation SNP Validation Relationship/Identification Specialist
Our motivation for a NGS/MPS SNP panel
Increasing the power in paternity and relationship testing utilizing MPS for the analysis of a large SNP panel Ida Grandell 1, Andreas Tillmar 1,2 1 Department of Forensic Genetics and Forensic Toxicology,
Jennifer D. Churchill, Maiko Takahashi, Christina Strobl, Dixie Peters, Christina Capt, Walther Parson, Bruce Budowle
Validation of a Massively Parallel Sequencing Workflow for Mitochondrial DNA Analysis at UNTHSC Center for Human Identification for Missing Persons and Traditional Casework Analyses Jennifer D. Churchill,
Evaluating Noise and the Implications of Methodology in the Analytical Threshold Designation for Forensic Genetic Analysis
University of North Texas Health Science Center UNTHSC Scholarly Repository Theses and Dissertations 8-1-2015 Evaluating Noise and the Implications of Methodology in the Analytical Threshold Designation
Incorporating SeqStudio Genetic Analyzer and Sanger sequencing into genome editing workflows
Incorporating SeqStudio Genetic Analyzer and Sanger sequencing into genome editing workflows Stephen Jackson, Ph.D 27 May 2017 The world leader in serving science Key Applications for Genome Editing Research
Generating Forensic DNA Profiles
Wright State University CORE Scholar Biological Sciences Faculty Publications Biological Sciences 12-2012 Generating Forensic DNA Profiles Dan E. Krane Wright State University - Main Campus, dan.krane@wright.edu
Titelstijl van model bewerken
Generate Titelstijl van and verify model your bewerken data Solutions for all your genetic analysis needs Sanger Sequencing Microarray technology QuantStudio real-time and digital PCR Ion Torrent NGS systems
STRmix Implementation and Internal Validation Erie County Central Police Services Forensic Laboratory
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 STRmix Implementation and Internal Validation-2015 Erie County Central Police Services Forensic Laboratory
Overview of the Ibis STR Assay
Thomas Hall, Ph.D. Overview of the Ibis STR Assay Outline Ibis STR assay format Primer design Database Assay layout Sample analysis Comparison to tradi/onal STR analysis Measures intrinsic property of
DNA methylation-based body fluid typing
DNA methylation-based body fluid typing Hwan Young Lee Department of Forensic Medicine Yonsei University College of Medicine, Seoul, Korea Forensic Body Fluid Identification Body fluid identification can
Complete protocol in 110 minutes Enzymatic fragmentation without sonication One-step fragmentation/tagging to save time
Molecular Cloning Laboratories Manual Version 1.2 Product name: MCNext UT DNA Sample Prep Kit Cat #: MCUDS-4, MCUDS-24, MCUDS-96 Description: This protocol explains how to prepare up to 96 pooled indexed
NGS Sample QC with the Agilent 2200 TapeStation. Rainer Nitsche Application Engineer Agilent Technologies, Inc.
NGS Sample QC with the Agilent 2200 TapeStation Rainer Nitsche Application Engineer Agilent Technologies, Inc. The Agilent 2100 Bioanalyzer First commercially available Lab-on-a-Chip product Introduced
Implementation of a More Efficient Workflow to Meet New Demands
Implementation of a More Efficient Workflow to Meet New Demands Cajsa Älgenäs, Head of Division, Division of Forensic Genetics, National Board of Forensic Medicin, Sweden RMV Government agency, Ministry
GENOMICS WORKFLOW SOLUTIONS THAT GO WHERE THE SCIENCE LEADS. Genomics Solutions Portfolio
GENOMICS WORKFLOW SOLUTIONS THAT GO WHERE THE SCIENCE LEADS Genomics Solutions Portfolio WORKFLOW SOLUTIONS FROM EXTRACTION TO ANALYSIS Application-based answers for every step of your workflow Scientists
Lab methods: Exome / Genome. Ewart de Bruijn
Lab methods: Exome / Genome 27 06 2013 Ewart de Bruijn Library prep is only a small part of the complete DNA analysis workflow DNA isolation library prep enrichment flowchip prep sequencing bioinformatics
BIOO LIFE SCIENCE PRODUCTS. NEXTflex TM 16S V4 Amplicon-Seq Kit 4 (Illumina Compatible) BIOO Scientific Corp V13.01
BIOO LIFE SCIENCE PRODUCTS NEXTflex TM 16S V4 Amplicon-Seq Kit 4 (Illumina Compatible) Catalog #: 4201-01 (16 reactions) BIOO Scientific Corp. 2013 V13.01 TABLE OF CONTENTS GENERAL INFORMATION... 1 Product
Validation of PowerPlex Y23 PCR Amplification System for Forensic Casework
Validation of PowerPlex Y23 PCR Amplification System for Forensic Casework Jessica Tokarz (B.S.) 1, Sarah Foster (B.S.) 2, Scott Milne (B.S.) 2, Pamela Staton (PhD) 1, Kathleen Press (M.S.F.S.) 3 1 Marshall
Accessible answers. Targeted sequencing: accelerating and amplifying answers for oncology research
Accessible answers Targeted sequencing: accelerating and amplifying answers for oncology research Help advance precision medicine Accelerate results with Ion Torrent NGS Life without cancer. This is our
FORENSIC GENETICS. DNA in the cell FORENSIC GENETICS PERSONAL IDENTIFICATION KINSHIP ANALYSIS FORENSIC GENETICS. Sources of biological evidence
FORENSIC GENETICS FORENSIC GENETICS PERSONAL IDENTIFICATION KINSHIP ANALYSIS FORENSIC GENETICS Establishing human corpse identity Crime cases matching suspect with evidence Paternity testing, even after
Dear Colleague, We look forward to working with you and helping you to reach your study goals. Best Regards, Your Illumina Genotyping Services Team
Dear Colleague, Thank you for using Illumina s SNP genotyping service. We are committed to your success. In addition to the actual genotyping, project success will be determined by SNP selection, DNA preparation,
Product selection guide Ion S5 and Ion S5 XL Systems
Product selection guide Ion S5 and s Cancer genomics research Molecular profiling Ion AmpliSeq Ready-to-Use Panels Ion AmpliSeq Comprehensive Cancer Panel Cat. No. 4477685 Ion AmpliSeq Made-to-Order Panels
Product selection guide Ion GeneStudio S5 Series
Cancer genomics research Molecular profiling Ion AmpliSeq Comprehensive Cancer Panel Cat. No. 4477685 Ion AmpliSeq Made-to-Order Panels (Customize your own or browse redesigned community panels at ampliseq.com)
GENOMICS WORKFLOW SOLUTIONS THAT GO WHERE THE SCIENCE LEADS. Genomics Solutions Portfolio
GENOMICS WORKFLOW SOLUTIONS THAT GO WHERE THE SCIENCE LEADS Genomics Solutions Portfolio WORKFLOW SOLUTIONS FROM EXTRACTION TO ANALYSIS Application-based answers for every step of your workflow Scientists
Reliable. answers, significant savings. Applied Biosystems QuantStudio 5 Real-Time PCR System for Human Identification
Reliable answers, significant savings Applied Biosystems QuantStudio 5 Real-Time PCR System for Human Identification Discover the QuantStudio 5 Real-Time PCR System for Human Identification The modern
Biology 445K Winter 2007 DNA Fingerprinting
Biology 445K Winter 2007 DNA Fingerprinting For Friday 3/9 lab: in your lab notebook write out (in bullet style NOT paragraph style) the steps for BOTH the check cell DNA prep and the hair follicle DNA
TIME IT S PRIME. chemagic TM Prime TM instrument Streamlined Walk-away Automation. For all Human Sample Materials. Primary Sample Handling
IT S PRIME TIME chemagic TM Prime TM instrument Streamlined Walk-away Automation. For all Human Sample Materials. Primary Sample Nucleic Acid Isolation Eluate Quantitation of Extracted Samples Assay Setup
John M. Butler and Peter M. Vallone National Institute of Standards and Technology
DNA Biometrics: i Standards and Technology John M. Butler and Peter M. Vallone National Institute of Standards and Technology NDIA Biometrics Conference (Arlington VA) NDIA Biometrics Conference (Arlington,
SNP GENOTYPING WITH iplex REAGENTS AND THE MASSARRAY SYSTEM
SNP GENOTYPING Accurate, sensitive, flexible MassARRAY System SNP GENOTYPING WITH iplex REAGENTS AND THE MASSARRAY SYSTEM Biomarker validation Routine genetic testing Somatic mutation profiling Up to 400
STUDY OF VNTR HUMAN POLYMORPHISMS BY PCR
STUDY OF VNTR HUMAN POLYMORPHISMS BY PCR Ref. PCR1 1. OBJECTIVE OF THE EXPERIMENT The objective of this experiment is to introduce students to the principles and practice of Polymerase Chain Reaction (PCR)
Male/female DNA mixtures: a challenge for Y-STR analysis
International Congress Series 1239 (2003) 295 299 Male/female DNA mixtures: a challenge for Y-STR analysis Burkhard Berger, Harald Niederstätter, Silvano Köchl, Martin Steinlechner, Walther Parson * Institute
SNP GENOTYPING WITH iplex REAGENTS AND THE MASSARRAY SYSTEM
SNP GENOTYPING Accurate, sensitive, flexible MassARRAY System SNP GENOTYPING WITH iplex REAGENTS AND THE MASSARRAY SYSTEM Biomarker validation Routine genetic testing Somatic mutation profiling Up to 400
Laboratory Validation. Chapter 16
Laboratory Validation Chapter 16 Importance The DNA profile is used to convict or free a suspect It must be perfect! No mistakes like we have in regular laboratories all the time Also DNA evidence must
DNA Analysis Students will learn:
DNA Analysis Students will learn: That DNA is a long-chain polymer found in nucleated cells, which contain genetic information. That DNA can be used to identify or clear potential suspects in crimes. How
Forensic genetics: New frontiers
Forensic genetics: New frontiers Dennis McNevin (CRICOS) #00212K DNA profiles First application of DNA profiling : The Enderby murder case A single locus minisatellite probe was used to analyze the following
Empowering genotyping by sequencing in animal applications - the power of partnering.
Empowering genotyping by sequencing in animal applications - the power of partnering. Round 1: About cats, dogs and cattle Wim van Haeringen, PhD Content Company Introduction Background including market
Technical Focus. Direct PCR Amplification of Buccal Samples on the NUCLEIC-CARD COLOR With the AmpFlSTR Identifiler Direct PCR Amplification Kit
Technical Focus Direct PCR Amplification of Buccal Samples on the COLOR With the AmpFlSTR Identifiler Direct PCR Amplification Kit Forensic News 2013 Alessandro Agostino 1, Annalisa Gervasoni 2, Alice
HID Validation: The 3500 Series Genetic Analyzers
Outline Scope Materials and Reagents Validation Elements Selection of Validation Data Additional Sources of Validation Information User Bulletin 2 Validation Scope Hardware and Reagents HID Validation:
A Rape on a Minor Victim Under POCSO Act 2012 Investigation Through DNA Analysis Technique A Case Study
A Rape on a Minor Victim Under POCSO Act 2012 Investigation Through DNA Analysis Technique A Case Study Rohidas K. Mundhe, Rajendra S. Mali, Sangeeta V. Ghumatkar and Madhukar K. Malve Abstract DNA profiling
STR Profiling Matching Criteria: Establishment and Importance of a Cell Line Database
STR Profiling Matching Criteria: Establishment and Importance of a Cell Line Database Margaret Kline Applied Group Biochemical Science Division Chemical Science and Technology Laboratory National Institute
Reagent Kit Selection Guide
Reagent Kit Selection Guide Introduction The BioChain Nucleic Acid Preparation Technology Introduction BioChain Institute Inc. specializes in developing advanced, efficient and reliable technologies in
Y chromosomal STRs in forensics
Haplotype and mutation analysis for newly suggested Y-STRs in Korean father-son pairs Yu Na Oh, Hwan Young Lee, Eun Young Lee, Eun Hey Kim, Woo Ick Yang, Kyoung-Jin Shin Department of Forensic Medicine
Embrace the Future of Electrophoresis
Embrace the Future of Electrophoresis Analysis of 12 samples in as little as 3 minutes Unattended analysis of up to 96 samples Resolution down to 3 5 bp for fragments
Agrigenomics solutions. Your partner for smarter agrigenomics solving challenges together
Agrigenomics solutions Your partner for smarter agrigenomics solving challenges together Our commitment to you We re dedicated to partnering with you to find the right solution for your needs. Whether
Next Generation Sequencing of HLA: Challenges and Opportunities in the era of Precision Medicine. Dr. Paul Keown, 2016
Next Generation Sequencing of HLA: Challenges and Opportunities in the era of Precision Medicine Dr. Paul Keown, 2016 Statement of Conflict & Collaboration Therapeutics collaborations Novartis, Roche,