Institute of Biotechnology and Genetic Engineering. Institute of Biotechnology and Genetic Engineering, KPK-AUP, Pakistan.

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1 IJAZ ALI PhD Assistant Professor Institute of Biotechnology and Genetic Engineering Khyber Pakhtunkhwa Agricultural University Peshawar Molecular Genetics Lab. Khyber Pakhtunkhwa, Pakistan Off Mob Fax com 1. RESEARCH INTERESTS Understanding the molecular aspects of Infectious diseases, molecular epidemiology, zoonotic diseases, stem cell biology, promoter analysis and genetic diversity studies. Special areas of interest include a. Infectious diseases. b. Molecular Biology. c. Invivo and Invitro Promoter studies. 2. FACULTY APPOINTMENTS Research Officer 1 National Centre Of Excellence in Molecular Biology, University of the Punjab, Pakistan Assistant Professor 2 Institute of Biotechnology and Genetic Engineering, KPK-AUP, Pakistan Date Assistant Professor 3 Institute of Biotechnology and Genetic Engineering, KPK-AUP, Pakistan. 3. HIGHER EDUCATION Doctor of Philosophy Graduate University of the Chinese Academy of Sciences, IOZ, Beijing, China Master of Philosophy National Centre Of Excellence in Molecular Biology, University of the Punjab, Pakistan Doctor of Veterinary Medicine College of Veterinary Sciences Lahore, Faculty of University of Agriculture Faisal Abad. 4. AWARDS and HONORS Khyber Pakhtunkhwa Provincial Government Scholarship for Graduation (Pak) Higher Education Commission of Pakistan's Scholarship for PhD-abroad (China) 2009 Best presentation prize at FAPUASA seminar in 2009 (Pak) 5. MEMBERSHIP Member of Pakistan Veterinary Council (Pak) 2009-Date Member of the National Curriculum Revision Committee (Pak) 2010 Temporary member of the board of study of the KUST Date Founder of the Society for Awareness, Prevention and Control of Infectious Diseases (SAPCID). 6. BOOKS 1. Farzana Rashid and Dr. Ijaz Ali An optimized expression of recombinant hepcidin using Pichia Pastoris. Publisher: VDM Verlag Dr.Muller Akteingesellshaft & Co.KG. Dudweiler

2 Landster 99, Saarbrucken, Germany ISBN Ijaz Ali and Farzana Rashid. Promoter Analysis in Transgenic models. Being processed for publication with VDM Verlag Dr.Muller Akteingesellshaft & Co.KG. Dudweiler Landster 99, 66123Saarbrucken, Germany 7. ORIGINAL RESEARCH PAPERS 1. Latif ur Rehman1, Ihasn Ullah1, Ijaz Ali1*, Imtiaz Ali Khan2, Aqib Iqbal1, Sanaullah Khan3, Sher Hayat Khan4, Khaleeq Uz Zaman1, Najib ullah Khan1, Zahoor Ahmed Swati1, Anila Tariq Jahangiri. Active hepatitis C infection and HCV genotypes prevalent among the IDUs of Khyber Pakhtunkhwa. Virology Journal 2011, 8: Ijaz Ali, Sanaullah Khan, Sobia Attaullah, Shahid N Khan, Jabbar Khan, Sami Siraj, Aqib Iqbal, Zahoor A Swati and Muhammad Idrees Response to combination therapy of HCV 3a Infected Pakistani patients and the role of NS5A protein. BMC Virology Journal 2011, 8: Ijaz Ali, Lubna Siddique, Iqbal Munir, Latifur rehman, Aqib Iqbal, Farzana Rashid, Zahoor Ahmad Swati Prevalence of HCV among the various risk groups in Khyber Pakhtunkhwa. BMC Virology Journal 8: Ijaz Ali, Muti Ur Rehman, Farzana Rashid, Sanaullah Khan, Aqib Iqbal, Xia Laixin, Naeem ud din Ahmed, A. Zahoor Swati Cis-regulatory elements affecting the Nanos gene promoter in the germline stem cells. Journal of Biotechnology, Elsevier, 145: Sajid Ali, Ijaz Ali, Bashir Ahmed Prevalence of common HCV genotypes among the chronic HCV patients in Khyber Pakhtunkhwa. BMC Virology Journal 8: Ijaz Ali, Farzana Rashid, Muhammad Arshad, Muti-ur-rehman and Atta-ur-rehman Ligation-mediated IR-PCR; a novel and easy way to make RNAi constructs. Journal of Biotechnology, Elsevier.136S (S183-S186). 7. Sobia Attaullah, Sanaullah Khan, Naseemullah., Sultan Ayaz, Shahid Niaz Khan, Ijaz Ali, Naseruddin Hoti and Sami Siraj Prevalence of HBV and HBV vaccination coverage in health care workers of tertiary hospitals of Peshawar, Pakistan. BMC Virology Journal, 8: Najib ullah, Ijaz Ali, Naeem ud din Ahmed, Aqib Iqbal 1, Iqbal Munir, Latif ur Rehman, Lubna Siddiq, Muti ur Rehman 2, Sajid Ali 4, Zahoor Ahmed Swati 1. Prevalence of active HCV infection among the blood donors of Khyber Pakhtunkwa and FATA region of Pakistan and evaluation of the screening tests for anti-hcv. BMC Virology Journal 2011, 8: Amjad Ali, Habib Ahmad, Ijaz Ali, Sheema Khan, Gulshan Zaidi, and Muhammad Idrees Prevalence of active hepatitis c virus infection in district mansehra Pakistan. BMC Virol Journal, 7: Farzana Rashid, Ijaz Ali, Yuan QP, Xiaoxin S, Fei L Secretory Expression and Purification of a Human Hepcidin in P. pastoris. Biotechnology Bulletin, (9), Sanaullah Khan, Sobia Attaullah, Sultan Ayaz, Shahid Niaz Khan, Sumaira Shahms, Ijaz Ali, Muhammad Bilal and Sami Siraj Molecular Epidemiology of HCV among the health care workers of Khyber Pakhtunkhwa Pakistan. BMC Virology Journal, 8: Shahid Ali, Iqbal Munir, Mushtaq Ahmad, Muhammad Arif, Ijaz Ali, Farhatullah, Sayed Jaffar Abbas, and Zahoor Ahmed Swati Characterization of Brassica napus germplasm based on molecular markers. African Journal of Biotechnology Vol. 10 (16), pp Farzana Rashid, Ijaz Ali and Qipeng Yuan Expression studies of Human anti-microbial peptide Hepcidin. Journal of Biotechnology, Elsevier. 136S (S541-S547). 14. Malik Nawaz Shuja, Wajid Ali, Aqib Iqbal, Ijaz Ali, Mohammad Iqbal, Gulzar Ahmad and Zahoor Ahmad Swati Maize breeding for marginal lands; an integrated approach to decipher response of maize lines to water deficit. African Journal of Biotechnology Vol. 10(18), pp Ihsan Ullah, Aqib Iqbal, Ijaz Ali, Iqbal Munir et al Role of dietary supplementation in the protein content of bovine milk. African Journal of Biotechnology, 10(18), pp Najib ullah khan, Lubna Siddique, Ijaz Ali*, Aqib Iqbal, Iqbal Munir, Farzana Rashid, Sajid Ali, Fazal Raziq, Zahoor Ahmad Swati Prevalence of Hepatitis B in the blood donors

3 of N-W.F.P and FATA regions and the current scenario of HBV Infection in Pakistan. African Journal of Biotechnology. 9 (37): Wisal M. Khan, Iqbal Munir, Farhatullah, M. Arif, Aqib Iqbal, Ijaz Ali, Dawood Ahmad, Mushtaq Ahmad, Afaq Mian, Jehan Bakht, Inamullah and Zahoor A. Swati. Assessment of genetic diversity of Brassica juncea germplasm using randomly amplified polymorphic DNA (RAPD) markers. African Journal of Biotechnology Vol. 10(19), pp Farzana Rashid, Ali I, Sajid I, Elamin S, Yuan QP Recombinant Expression of an Antimicrobial Peptide Hepcidin in Pichia pastoris. Pakistan Journal of Zoology, 41(5), Nazir ud din, Saifullah, Musharraf Khan, Ijaz Ali, Aqib Iqbal Genetic characterization of Verticilium chlamygosporium isolated from Khyber Pakhtunkhwa using RAPD primers. Accepted in Pakistan Journal of Botany. 20. Muti ur Rehman Khan, Ijaz Ali, Pao-Jun Zhang, Yong Zhao, Meifu Feng. Ex vivo expansion of functional human UCB-HSCs/HPCs by co-culture with AFT024- hkirre cells. Accepted in Acta Biochemica et Biophysica Sinica. 21. Muhammad Riaz, Zumir Ahmed Ansari, Fouzia Iqbal, Ijaz Ali, Tauqeer Ahmad and Muhammad Akram Influence of Medium Components and Physical Factors on Vitamin B12 Production by Pseudomonas sp. PCSIR 99. Pak. J. Sci. Ind. Res. 50(6) Rashid Mehmood, Ijaz Ali, Fareeha Zulfiqar, Sheikh Riazuddin Prevalence of mutations in PDE6A gene in families affected with autosomal recessive retinitis pigmentosa. Biologia Pakistan 51: Naeem ud din Ahmad, Ijaz Ali, Sardar Khan, Shokat Ali, Sher Aslam, and Liu Ying Microbial Diversity in a Malachite Green Dye Treating Sequencing Batch Reactor. Under review in Pakistan Journal of Zoology. 24. Rizwan Alam, Aqib Iqbal, Ijaz ali, Arshad Javed, Iqbal Munir, Zahoor Ahmad Swati, Ikhtiar Khan Exogenous Ca2+ and K+ application enhanced membrane stability, chlorophyll content and water relation in Brassica napus seedlings under drought stress. Under Review in Plant Physiology and Biochemistry. 25. Farzana Rashid, Ijaz Ali, Sanaullah Khan, Muhammad Riaz, Qipeng Yuan Mutations within the HCV NS5A and its correlation with treatment response in Hepatitis C virus subtype 3a infected patients. BNPST, Farzana Rashid, Ijaz Ali, Imran Sajid, Qipeng Yuan Pishia pastoris a facilitated expression host for the liver expressed protein. BNPST, CONFERENCES/WORKSHOPS 1. Ijaz Ali, Aqib Iqbal and Zahhor A Swati Transcriptional regulation of drosophila nanos gene. Oral presentation in the 1st National Conference on Biotechnology and Microbiology, Baragali, 3-7 July Aqib Iqbal, R Alam, Ijaz Ali, Iqbal Munir, I Javed, Zahoor A Swati Exogenous Ca2+ and K+ application enhanced membrane stability, chlorophyll content and water relation in Brassica napus seedlings under drought stress. Oral presentation in the 1st National Conference on Biotechnology and Microbiology, Baragali, 3-7 July Najibullah Khan, Ijaz Ali, Aqib Iqbal, Iqbal Munir, Lubna Siddiq, Safira Attache, Latif ur Rehman, Zahoor Ahmed Swati Evaluation of ICT, ELISA and Real-time PCR for the detection of HCV infection. Poster presentation at the 3 rd symposium on building effective coordination between R&D and Industry. KPK. 4. Lubna Siddiq, Ijaz Ali, Aqib Iqbal, Iqbal Munir, Latif ur Rehman, Zahoor Ahmed Swati Evaluation of risk factors for HCV infection in Peshawar. Poster presentation at the 3 rd symposium on building effective coordination between R&D and Industry. KPK. 5. Teka Khan, Waheed ullah, Aqib Iqbal, Iqbal Munir and Ijaz Ali Evaluation of milk as an alternative source of DNA for genetic diversity studies. Poster presentation at the 3 rd symposium on building effective coordination between R&D and Industry. KPK. 6. Farzana Rashid, Ijaz Ali, Imran Sajid, Qipeng Yuan. Pishia pastoris a facilitated expression host for the liver expressed protein. Oral presentation at International symposium on Advanced Bio, Nano and Pharmaceutical Science and Technology (BNPST), May 3-6, BUCT, Beijing, China. 7. Farzana Rashid, Ijaz Ali, Sanaullah Khan, Muhammad Riaz, Qipeng Yuan. Mutations within the HCV NS5A and its correlation with treatment response in Hepatitis C virus subtype 3a infected patients. Oral presentation at International symposium on Advanced

4 Bio, Nano and Pharmaceutical Science and Technology (BNPST), May 3-6, BUCT, Beijing, China. 8. Ijaz Ali, Muti ur Rehman, Farzana Rashid, Xia Laixin, Chen Dongsheng, Qipeng Yuan. Cis-regulatory elements affecting the nanos gene promoter in the germline stem cells. Accepted for presentation and publication in ISBBE Ijaz Ali, Farzana Rashid, Qipeng Yuan. Resistance to Interferon based therapy in Hepatitis C virus subtype 3a infected Pakistani patients. ISBBE Ijaz Ali. Educational issues in war-effected Swat. PUTA/FAPUASA seminar on Educational policy. July Organized and conducted workshop on Applications and Techniques of Biotechnology and Genetic Engineering 2009 from July 27 August 01, at the Institute of Biotechnology and Genetic Engineering, AUP. 12. Farzana Rashid, Ijaz Ali and Qipeng Yuan. Expression studies of Human anti- microbial peptide Hepcidin. Oral presentation at IBS-2008 Dalian China. 13. Ijaz Ali, Farzana Rashid, Muhammad Arshad, Muti-ur-rehman and Atta-ur-rehman. Ligation-mediated IR-PCR; a novel and easy way to make RNAi constructs. Poster presentation at IBS-2008 Dalian China. 9. ARTICLES IN DAILY NEWS PAPERS 1. Educational problems in Swat. The frontier post daily, August 22, The dying culture of Pakhtuns. The frontier post daily, November 09, Pakhtuns will survive. The frontier post daily, December 01, 'HEC and the rivals' and other articles on social media. 10. GENBANK SUBMISSIONS Ali, I., Mehmood, R., Ghafoor, A., Khan, S., Mehmood, T., Idrees, M., Riaz, M. and Riazuddin, S Hepatitis C virus Pakistani isolates polyprotein mrna. GenBank accession numbers: 1. DQ DQ DQ DQ DQ DQ RESEARCH PROJECTS 1. Utilizing indigenous wild species from the arid areas of Pakistan to genetically engineer abiotic stress tolerance in crop plants (Co-author). Approved by Pakistan Science Foundation. 2. Research project on Brucellosis is under review with Pakistan Science Foundation. 3. Molecular epidemiology of Echinococcosis among humans and animal species. Under review with Higher Education Commission of Pakistan. 4. Genomic analysis of the Untypeable HCV genotype prevalent in Pakistan. Under review with Higher Education Commission of Pakistan. 5. Two IBGE funded projects on prevalence of HBV and Evaluation of HCV risk factors have been completed. 6. Research project on Transcriptional regulation of genes involved in stem cells maintenance has been completed in the Chinese Academy of Sciences. 7. Development of duplex PCR and RFLP-based genotyping for Avian Adeno Virus, Chicken Anaemia Virus, Mycoplasma species and ILTV has been completed. The project was sponsored by Big Bird and Grand-Parent Laboratories, Pakistan. 12. CURRICULUM DEVELOPMENT As a member of the National Curriculum Revision Committee, I have designed the following courses for B.Sc Hons Biotechnology in Pakistani universities, 1. Health Biotechnology. 2. Genetics-I (practical). 3. Elements of Biotechnology.

5 13. STUDENT'S SUPERVISION RESEARCH 1. Ihsanullah: Determination of active HCV infection and Corelation between Liver enzyme profiles and HCV viral laod among the IDUs in Peshawar (M.Phil thesis completed). 2. Lubna Siddique: Evaluation of risk factors for HCV infection in Peshawar (M.Phil thesis completed) 3. Prevalence of active HCV infection in district Mansehra (M.Phil thesis completed) Hazrat Hussain: Genetic diversity among the peacock breeds of Pakistan (M.Phil Research work completed). 5. Ruqayya: Prevalence of anti-hcv antibodies among the general population of Mardan (M.Phil research, Completed). 6. Karim (Hazara University): Genetic diversity among Balkhi and Rambolet sheep breeds (M.Phil research, ongoing). 7. Shaista: Genetic diversity among the sheep breeds of N-W.F.P using SSR markers (M.Phil research, Completed). 8. Khalid Hussain: Genetic diversity among the Cattle breeds of Khyber Pakhtunkhwa (M.Phil research, ongoing). 9. Teka Khan (Hazara University): Genetic diversity among and between the indigenous and exotic Cattle breeds using milk samples (M.Phil research completed) (Cosupervised) 10. Asim: Evaluation of HCV risk factors in Swat (M.Phil research, Ongoing). (Cosupervising). 11. Shafi ullah (Hazara University): Morphology and genetic analysis of peafowls in KPK. (M.Phil research, Ongoing). (Co-supervising). 12. Najibullah Khan: Response rate of combination therapy in HCV 3a infected patients (M.Phil research completed). 13. Latif ur Rehman: Development of a duplex PCR assay for the detection of Avian Adenovirus and Chicken Anemia Virus prevalent in Pakistan (M.Phil research completed). 14. Khaliq Uz Zaman: Development of a multiplex PCR assay for the detection of Mycoplasma specie (MG & MS) and ILT virus (M.Phil research completed). 15. Najibullah Khan: Prevalence of active HBV infection among the blood donors of NWFP and FATA region and the overall scenario of HBV infection in Pakistan (B.Sc Hons report completed and published). 16. Latifur rehman: Detection of Salmonella Enteritidis in food samples collected from University campuses (BSc Hons report completed). 17. Faiz Ali Khan: Evaluation of water and milk samples for bacterial pathogens (BSc Hons Report completed). 18. Ghazanfar Ilyas: Morphological characterization of Afghan Watani sheep breed and optimization of SSR-PCR for sheep (BSc Hons Report completed). 19. Sadiq shah: Morpholoical characterization of Waziri and Damani sheep breeds and tools for their genetic analysis (BSc Hons Report completed). 20. Rafi ur Rehman: Morphological characterization of Kutta breed and optimization of RAPD- PCR for sheep blood (BSc Hons Report completed). 21. Muhammad Fakhrul Alam: Morphological characterization of Karri sheep breed and optimization of RAPD-PCR for sheep blood (BSc Hons Report completed). 22. Shan Justin (Late): Morphological characterization of Balkhi breed and optimization of SSR- PCR for sheep blood (BSc Hons Report completed). 23. Sanaullah Khan (KUST): Dynamics of HCV infection in Khyber Pakhtunkhwa (PhD research, Ongoing).(Co-supervising) 24. Shahid Niaz Khan (KUST): Molecular epidemiology and risk factors of malaria infection in KPK (PhD research, Ongoing).(Co-supervising) 25. Sumaira Shams (KUST): Epidemiology, molecular detection and and control measures of babesiosis in cattle (PhD research, Ongoing). (Co-supervising). 26. Sajid Ali (KUST): Response to combination therapy and PegEsay in Hepatitis C virus infected patients of Khyber Pakhtunkhwa (PhD research, Ongoing). (Cosupervising).

6 14. COURSES TAUGHT AT IBGE a. Health Biotechnology (B.Sc Hons). b. Animal Biotechnology (B.Sc Hons). c. Genetics-I (M.Phil) d. Metabolism-I (B.Sc Hons). e. Metabolism-II. (B.Sc Hons) f. Biotechnology-I (B.Sc Hons). g. Advanced Molecular Biology (PhD). h. Lab Techniques (Ph.D). 14. FOOTNOTES [1]. Contract appointment. [2]. Adhoc appointment for 7 months followed by Tenure-track appointment under the IPFP program of the Higher Education Commission of Pakistan. [3]. Regular tenure-track appointment. : Supervisor or Co-supervisor of M.Phil/PhD Thesis.

Faculty Resume AQIB IQBAL

Faculty Resume AQIB IQBAL Faculty Resume Name Personal AQIB IQBAL H.No 157, Street 7, Sector J-5, Phase II, Hayatabad, Peshawar Phone: +92-334-9086080 E.mail.: aqib72@aup.edu.pk Experience May 2009 to date, Assistant Professor,