Their Metabolites in Salmon, Catfish, and. Action Method : Collaborative Study. WENDY C. ANDERSEN FDA - Animal Drugs Research Center, CO

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1 Determination of Triphenylmethane Dyes and Their Metabolites in Salmon, Catfish, and Shrimp by LC-MS/MS using AOAC First Action Method : Collaborative Study WENDY C. ANDERSEN FDA - Animal Drugs Research Center, CO MARILYN J. SCHNEIDER USDA - Agricultural Research Service, Eastern Regional Research Center, PA

2 AOAC SMPR Quantitative Methods for Drug Residues in Shrimp, Tilapia, Catfish and Salmon Approved by the AOAC Stakeholders Panel on Seafood Intended Use: Laboratory use by qualified analysts for analysis of tissues with Maximum Levels (ML) < 1 mg/kg g(pp (ppm) Applicability: Shrimp, Tilapia, Catfish, and Salmon.

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4 Study Design SMPR Stakeholders - method applicable to shrimp, tilapia, catfish and salmon First Action method validation for trout matrix Matrix effects for shrimp, tilapia, salmon, pangasius (n=6 ) studied in complete ANSES work (Hurtaud-Pessel, Coeudor, Verdon J. Chromatogr. B, 2011) Test method at FDA Denver Validate method for shrimp, tilapia, catfish, and salmon; test stability Obtain incurred samples from FDA-CVM; incurred shrimp not available

5 Study Design 1997 AOAC Official Methods Program Manual 14 laboratories reporting valid data 5 analytes (MG, LMG, CV, LCV, and BG) 3 matrices (shrimp, catfish, and salmon) 5 concentration levels blinded samples in duplicate Level of interest is 1.0 g/kg g (ppb) Blank Spikes 0.42, 0.90, and 1.75 g/kg Incurred Designed to also meet requirements of an FDA level four chemical method validation

6 Timeline Sept 2013 AOAC Vet Drugs Subgroup Study began Nov Initial study design; incurred tissue Dec/Jan Test method and stability studies January Invite participants (14 labs), finalize study design and protocol February Prepare materials (homogenize matrix, standard solutions, blinded test portions, labels, etc) 3 weeks to optimize equipment and practice Feb 25 Ship study materials to 14 labs March 19 Labs complete analysis (3 analysis days per lab) April 4 Final data submitted by labs

7 May 5 weeks to evaluate data: AB Sciex 5500 QTrap (5), AB Sciex 4000 QTrap (2), AB Sciex 3000, Agilent 6490 (2), Agilent 6460, Thermo TSQ Vantage, Thermo Quantum Discovery Max, Waters Micromass Quattro Micro API, Waters Acuity TQD (1), Waters Quattro Premiere XE June - 4 weeks for statistical analysis: John Phillips, statistician at USDA/ARS/ERRC July Aug - additional data analysis: Residue identification (product ion ratios, RT), detection limits (MDL, LOQ, CC, CC method screening, calibration methods August finalize manuscript: JAOACInt(submitted J. Int. Aug 28)

8 Results All14lb labs completed ltdthe Study Std Extracted matrix curves with internal standard correction, n = 51 samples per lab Method performed very well: Compound % Recovery % RSD r Malachite Green Leucomalachite Green Crystal Violet Leucocrystal Violet Brilliant Green HorRat generally <1 Recommend AOAC for Final Action

9 US State Labs Mark Crosswhite and Walter Hammack Florida Dept. of Agriculture & Consumer Services Christopher K. Lam and Mary Cumming Tennessee Department of Agriculture Mike Filigenzi and Robyn Noonan CA Animal Health and Food Safety Lab, UC Davis US Federal Labs Alan R. Lightfield and Steven J. Lehotay USDA-ARS Eastern Regional Research Center, PA Sara Lupton USDA-ARS Biosciences Research Laboratory, Fargo, ND Robert Burger FDA/ORA Denver Laboratory Yanxuan Tina Cai FDA/ORA Northeast Regional Laboratory Andrew Fong, John Kibbey, and Chris A. Baker FDA/ORA Arkansas Regional Laboratory Haejung An FDA/ORA Pacific Regional Laboratory Southwest International Labs Dominique Hurtaud-Pessel, Pierrick Couëdor and Eric Verdon ANSES, Laboratory of Fougères, Fougères, France Jason Smith and Ross Potter Canadian Food Inspection Agency, Nova Scotia, Canada Andrew Harris and Linda Lissemore University of Guelph, Laboratory Services, Guelph, Ontario, Canada Pi Private Labs Lb Sarah R. Ruiz and Cheryl D. Stephenson Eurofins Central Analytical Laboratories, LA Anna Hawkins, Shristi Prakash and Michael Conway OMIC USA Inc., Portland OR